pycom08g02520

S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0013169
Physical Location & Seq
Forward (+)
1852548 .. 1855575
3028 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g02520.6

Sequence Viewer

Length: 1926 bp
ATGTCCCTCTCCTCCACCCCTGCGCGGCTCACTCTCCTCGCCCTCCTCTCCGCCACCACAGTCTACTGCTTCTACAAATCCCGCCGCCTCCGACGCCTCAAGCTCTCCCTAAACCCTAACTCTAGCTCCTCGCCTCGCAGAGGCAAGCTCTTCTTCGTCTCCCAAACCGGCACCTCCGAAGCTCTAGCTCGTCGCCTGCACGATTTTCTGACGTCGAACGGCGTCGCTTTCGACCTCGTCGACCCCAACAACTACGAGCCCGAGGACCTGCCGAAGGAAACCCTAGTTCTGATTGTCGCTTCGACGTGGGATGACGGGAAGCCGCCTCCGAACGCCAGATTCTTCTCCAATTGGCTCGCCGAGAGTGCGGAGGACTTCAGGGTGGGATCGCTGTTGCTCTCCAATTGCAAATTTGCCGTCTTTGGCGTCGGGAGCCGAGCCTACGGCGCTACGTTCAATGCGGTGGGGAAGGACTTGGCTAGGAGGATGAGGGCGTTGGGCGCTTCTGAGATGTTTCCGATTTGGGAAGGTGACGTGGACGGTGGTGATTTGGACGAGGTGTTTGATGCTTGGAGTGGGAAGGTGGTGAGGTTTCTGAAGGGTGGTGCGGCGGAAAATGGCGGGGTTTTGGGTAATGGGGTTGGCGATGAATGTGAAGCTGAGAGCTTTGATGGGTCGGACGAGGAGGAGGAGAACGACGAGGATGGTGGGGTTGTTGACCTTGAGGATATTGCAGGTAAAGGACCTTCAAGAAGGAATCCAGTGGCGGTGACGAAGACTAATGGGACAGTGAATGGTAAAAAAGAGATGGTCACTCCGGTGATTCGGGCTAGCTTAACGAAGCAGGGGTATAAAATCATTGGTTCACATAGCGGCGTCAAAATTTGTAGATGGACAAAGTCGCAACTCCGAGGCCGTGGAGGTTGCTACAAGCACTCGTTTTATGGCATAGAAAGTCACAGGTGCATGGAGACAACCCCTAGTTTGGCATGTGCCAACAAATGTGTTTTTTGTTGGAGACATCACACAAATCCTGTCGGAAAGAGTTGGCAGTGGAAGATGGATGATCCGTTAGAAATTGTTAACTCTGCCATAGACCAGCACATGAAAATGATTAAACAAATGAAAGGAGTTCCTGGTGTTACATTGGAGCGATTAACAGAGGGTCTCTCTCCCAGGCATTGCGCCTTATCTCTTGTTGGTGAACCTATCATGTATCCTGAGATTAATGCACTCGTAGATGAGCTGCATCGAAGGCGGATTTCTACTTTTCTAGTCACAAATGCTCAGTTTCCTGAAAAGATCAAAATGCTGAACCCCATTACGCAGTTATATGTAAGTGTGGATGCTGCTACTAAAGAAAGTTTGAAGGCAATTGACAGGCCACTTTTTGGGGATTTTTGGGAGCGATTCATTGATTCCTTGACTTCTCTCAAGGACAAACAGCAACGAACTGTTTATCGCCTTACTCTGGTGAAAGGCTGGAATACAGAAGATATTGATGCTTATTATAACATTTTTAGCCTTGGTAGTCCTGATTTTGTTGAAATCAAGGGAGTTACATACTGTGGAACGTCTGCTACATCAAAGTTGACAATGGAGAATGTGCCCTGGCATGCCGATGTTAAAGCATTTTCAGAGGCCTTAGCTTTGAAAAGTAACGGGGACTATGAAGTTGCATGTGAGCATGTCCACTCTTGTTGTGTCCTCCTGGCTAAAACTAGCAAGTTCAAGATCAACGGCCAGTGGTTCACGTGGATTGACTATGAAAAGTTTCACGATCTGGTGGCTTCTGGAAAACCTTTTGACAGTAAGGATTACATGGCTCCAACACCTTCATGGGCCGTGTATGGGGCAGATGAAGGTGGATTTGATCCGTACCAGTCTCGATACAAGAAAGAGCGCCATCACAGACCAAACCAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Flavodoxin_1 Radical_SAM Wyosine_form

Protein Analysis

642

Amino Acids

71.47

Weight (kDa)

6.95

Isoelectric Point (pI)

37.5

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013169)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G75200
fragaria_vesca FvH4_2g39810 FvH4_2g39810
malus_domestica MD08G1031900.v1.1 MD15G1027800.v1.1
prunus_persica Prupe.1G379500_v2.0.a1 Prupe.1G379500_v2.0.a1
pyrus_communis pycom08g02520 pycom15g02380
rosa_chinensis RchiOBHm_Chr6g0301031
rosa_laevigata RLG00000011283
rosa_multiflora Rmu_co8483439.1_g000001
rosa_roxburghii Rroxscaffold_7G00167240
rosa_rugosa Rorug06G0300100
rosa_samantha Rh6AG414000 Rh6BG421000 Rh6CG428600 Rh6DG414000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1512
AatII GACGTC 1 cut(s) 215
Acc36I ACCTGC 2 cut(s) 276, 725
AccB1I GGYRCC 1 cut(s) 170
AccB7I CCANNNNNTGG 1 cut(s) 1391
AccI GTMKAC 2 cut(s) 63, 240
AccII CGCG 1 cut(s) 25
AclWI GGATC 3 cut(s) 394, 1061, 1868
AcoI YGGCCR 1 cut(s) 1741
AcsI RAATTY 2 cut(s) 410, 882
AcuI CTGAAG 2 cut(s) 361, 617
AcvI CACGTG 1 cut(s) 1755
AcyI GRCGYC 5 cut(s) 94, 212, 222, 426, 876
AfaI GTAC 1 cut(s) 1880
AfiI CCNNNNNNNGG 7 cut(s) 24, 140, 274, 985, 1391, 1471, 1851
AgsI TTSAA 6 cut(s) 457, 750, 1369, 1547, 1654, 1732
AjiI CACGTC 2 cut(s) 306, 535
AjnI CCWGG 4 cut(s) 1135, 1175, 1610, 1710
AleI CACNNNNGTG 1 cut(s) 818
Alw26I GTCTC 5 cut(s) 163, 965, 1012, 1172, 1890
AlwI GGATC 3 cut(s) 394, 1061, 1868
Ama87I CYCGRG 1 cut(s) 260
AoxI GGCC 5 cut(s) 913, 1382, 1641, 1741, 1842
ApeKI GCWGC 2 cut(s) 1246, 1349
ApoI RAATTY 2 cut(s) 410, 882
ArsI GACNNNNNNTTYG 4 cut(s) 402, 434, 545, 577
AseI ATTAAT 1 cut(s) 1227
Asp700I GAANNNNTTC 1 cut(s) 1773
AspLEI GCGC 5 cut(s) 25, 449, 503, 1187, 1905
AspS9I GGNCC 3 cut(s) 265, 743, 1842
AsuHPI GGTGA 7 cut(s) 542, 557, 598, 781, 832, 1214, 1486
AsuNHI GCTAGC 1 cut(s) 830
AvaI CYCGRG 1 cut(s) 260
AvaII GGWCC 2 cut(s) 265, 743
BaeGI GKGCMC 1 cut(s) 1611
BanI GGYRCC 1 cut(s) 170
BanII GRGCYC 1 cut(s) 261
BarI GAAGNNNNNNTAC 2 cut(s) 730, 762
BbrPI CACGTG 1 cut(s) 1755
BbsI GAAGAC 1 cut(s) 782
BbvI GCAGC 2 cut(s) 1233, 1336
BccI CCATC 6 cut(s) 665, 698, 802, 885, 1054, 1914
BceAI ACGGC 6 cut(s) 235, 401, 460, 900, 1756, 1829
BciT130I CCWGG 4 cut(s) 1137, 1177, 1612, 1712
BciVI GTATCC 1 cut(s) 1227
BcoDI GTCTC 5 cut(s) 163, 965, 1012, 1172, 1890
BfaI CTAG 8 cut(s) 123, 185, 284, 480, 831, 981, 1274, 1722
BfoI RGCGCY 3 cut(s) 450, 504, 1906
BfuAI ACCTGC 2 cut(s) 276, 725
BfuI GTATCC 1 cut(s) 1227
BisI GCNGC 7 cut(s) 26, 85, 323, 609, 874, 1247, 1350
BlsI GCNGC 7 cut(s) 27, 86, 324, 610, 875, 1248, 1351
Bme1390I CCNGG 4 cut(s) 1137, 1177, 1612, 1712
Bme18I GGWCC 2 cut(s) 265, 743
BmeT110I CYCGRG 1 cut(s) 260
BmgBI CACGTC 2 cut(s) 306, 535
BmgT120I GGNCC 3 cut(s) 265, 743, 1842
BmiI GGNNCC 3 cut(s) 172, 434, 1827
BmrFI CCNGG 4 cut(s) 1137, 1177, 1612, 1712
BmsI GCATC 4 cut(s) 556, 1258, 1336, 1492
BmtI GCTAGC 1 cut(s) 834
BpiI GAAGAC 1 cut(s) 782
BplI GAGNNNNNCTC 4 cut(s) 132, 164, 1154, 1186
Bpu10I CCTNAGC 1 cut(s) 1645
BpuEI CTTGAG 3 cut(s) 83, 743, 1418
BsaAI YACGTR 1 cut(s) 1755
BsaHI GRCGYC 5 cut(s) 94, 212, 222, 426, 876
BsaI GGTCTC 1 cut(s) 1172
BsaJI CCNNGG 6 cut(s) 261, 910, 916, 1175, 1525, 1610
BsaWI WCCGGW 1 cut(s) 817
BsaXI ACNNNNNCTCC 2 cut(s) 110, 140
Bsc4I CCNNNNNNNGG 7 cut(s) 24, 140, 274, 985, 1391, 1471, 1851
Bse118I RCCGGY 1 cut(s) 167
Bse1I ACTGG 4 cut(s) 761, 1744, 1882, 1921
Bse3DI GCAATG 1 cut(s) 1180
BseBI CCWGG 4 cut(s) 1137, 1177, 1612, 1712
BseDI CCNNGG 6 cut(s) 261, 910, 916, 1175, 1525, 1610
BseGI GGATG 5 cut(s) 316, 492, 709, 1069, 1351
BseLI CCNNNNNNNGG 7 cut(s) 24, 140, 274, 985, 1391, 1471, 1851
BseMI GCAATG 1 cut(s) 1180
BseMII CTCAG 4 cut(s) 498, 651, 1212, 1301
BseNI ACTGG 4 cut(s) 761, 1744, 1882, 1921
BseRI GAGGAG 6 cut(s) 26, 35, 118, 698, 701, 704
BseSI GKGCMC 1 cut(s) 1611
BseXI GCAGC 2 cut(s) 1233, 1336
BsgI GTGCAG 1 cut(s) 182
Bsh1236I CGCG 1 cut(s) 25
BshFI GGCC 5 cut(s) 915, 1384, 1643, 1743, 1844
BshNI GGYRCC 1 cut(s) 170
BsiHKCI CYCGRG 1 cut(s) 260
BsiSI CCGG 2 cut(s) 168, 818
BslFI GGGAC 2 cut(s) 799, 1679
BslI CCNNNNNNNGG 7 cut(s) 24, 140, 274, 985, 1391, 1471, 1851
BsmAI GTCTC 5 cut(s) 163, 965, 1012, 1172, 1890
BsmBI CGTCTC 1 cut(s) 163
BsmFI GGGAC 2 cut(s) 799, 1679
BsnI GGCC 5 cut(s) 915, 1384, 1643, 1743, 1844
Bso31I GGTCTC 1 cut(s) 1172
BsoBI CYCGRG 1 cut(s) 260
Bsp1286I GDGCHC 2 cut(s) 261, 1611
Bsp143I GATC 6 cut(s) 386, 1066, 1302, 1734, 1780, 1873
BspANI GGCC 5 cut(s) 915, 1384, 1643, 1743, 1844
BspCNI CTCAG 4 cut(s) 499, 652, 1213, 1300
BspFNI CGCG 1 cut(s) 25
BspLI GGNNCC 3 cut(s) 172, 434, 1827
BspMI ACCTGC 2 cut(s) 276, 725
BspOI GCTAGC 1 cut(s) 834
BspPI GGATC 3 cut(s) 394, 1061, 1868
BspQI GCTCTTC 1 cut(s) 155
BspT107I GGYRCC 1 cut(s) 170
BspTNI GGTCTC 1 cut(s) 1172
BsrDI GCAATG 1 cut(s) 1180
BsrFI RCCGGY 1 cut(s) 167
BsrI ACTGG 4 cut(s) 761, 1744, 1882, 1921
BssAI RCCGGY 1 cut(s) 167
BssECI CCNNGG 6 cut(s) 261, 910, 916, 1175, 1525, 1610
BssMI GATC 6 cut(s) 386, 1066, 1302, 1734, 1780, 1873
BssNI GRCGYC 5 cut(s) 94, 212, 222, 426, 876
BssT1I CCWWGG 1 cut(s) 1525
Bst2UI CCWGG 4 cut(s) 1137, 1177, 1612, 1712
Bst4CI ACNGT 6 cut(s) 61, 542, 790, 1456, 1568, 1811
Bst6I CTCTTC 1 cut(s) 155
BstACI GRCGYC 5 cut(s) 94, 212, 222, 426, 876
BstBAI YACGTR 1 cut(s) 1755
BstC8I GCNNGC 5 cut(s) 146, 197, 357, 832, 1617
BstDEI CTNAG 5 cut(s) 507, 660, 1221, 1287, 1645
BstDSI CCRYGG 1 cut(s) 916
BstENI CCTNNNNNAGG 2 cut(s) 138, 272
BstF5I GGATG 5 cut(s) 316, 492, 709, 1069, 1351
BstFNI CGCG 1 cut(s) 25
BstH2I RGCGCY 3 cut(s) 450, 504, 1906
BstHHI GCGC 5 cut(s) 25, 449, 503, 1187, 1905
BstKTI GATC 6 cut(s) 389, 1069, 1305, 1737, 1783, 1876
BstMAI GTCTC 5 cut(s) 163, 965, 1012, 1172, 1890
BstMBI GATC 6 cut(s) 386, 1066, 1302, 1734, 1780, 1873
BstMWI GCNNNNNNNGC 6 cut(s) 93, 365, 432, 446, 500, 1255
BstNI CCWGG 4 cut(s) 1137, 1177, 1612, 1712
BstNSI RCATGY 4 cut(s) 993, 1619, 1683, 1691
BstSCI CCNGG 4 cut(s) 1135, 1175, 1610, 1710
BstSLI GKGCMC 1 cut(s) 1611
BstUI CGCG 1 cut(s) 25
BstV1I GCAGC 2 cut(s) 1233, 1336
BstV2I GAAGAC 1 cut(s) 782
BsuI GTATCC 1 cut(s) 1227
BsuRI GGCC 5 cut(s) 915, 1384, 1643, 1743, 1844
BtgI CCRYGG 1 cut(s) 916
BtgZI GCGATG 1 cut(s) 660
BtrI CACGTC 2 cut(s) 306, 535
BtsCI GGATG 5 cut(s) 316, 492, 709, 1069, 1351
BtsI GCAGTG 1 cut(s) 1058
BtsIMutI CAGTG 4 cut(s) 768, 795, 1058, 1751
BveI ACCTGC 2 cut(s) 276, 725
Cac8I GCNNGC 5 cut(s) 146, 197, 357, 832, 1617
CfoI GCGC 5 cut(s) 25, 449, 503, 1187, 1905
Cfr10I RCCGGY 1 cut(s) 167
Cfr13I GGNCC 3 cut(s) 265, 743, 1842
CseI GACGC 4 cut(s) 102, 211, 415, 865
Csp6I GTAC 1 cut(s) 1879
CspCI CAANNNNNGTGG 2 cut(s) 1682, 1717
CviAII CATG 9 cut(s) 967, 990, 1105, 1213, 1616, 1680, 1688, 1822, 1839
CviQI GTAC 1 cut(s) 1879
DdeI CTNAG 5 cut(s) 507, 660, 1221, 1287, 1645
DpnI GATC 6 cut(s) 388, 1068, 1304, 1736, 1782, 1875
DpnII GATC 6 cut(s) 386, 1066, 1302, 1734, 1780, 1873
EaeI YGGCCR 1 cut(s) 1741
Eam1104I CTCTTC 1 cut(s) 155
EarI CTCTTC 1 cut(s) 155
EciI GGCGGA 3 cut(s) 40, 626, 1273
Eco130I CCWWGG 1 cut(s) 1525
Eco147I AGGCCT 1 cut(s) 1643
Eco24I GRGCYC 1 cut(s) 261
Eco31I GGTCTC 1 cut(s) 1172
Eco47I GGWCC 2 cut(s) 265, 743
Eco57I CTGAAG 2 cut(s) 361, 617
Eco72I CACGTG 1 cut(s) 1755
Eco88I CYCGRG 1 cut(s) 260
EcoNI CCTNNNNNAGG 2 cut(s) 138, 272
EcoO109I RGGNCCY 2 cut(s) 265, 743
EcoRII CCWGG 4 cut(s) 1135, 1175, 1610, 1710
EcoT14I CCWWGG 1 cut(s) 1525
EcoT38I GRGCYC 1 cut(s) 261
ErhI CCWWGG 1 cut(s) 1525
Esp3I CGTCTC 1 cut(s) 163
FaeI CATG 9 cut(s) 970, 993, 1108, 1216, 1619, 1683, 1691, 1825, 1842
FalI AAGNNNNNCTT 2 cut(s) 137, 169
FaqI GGGAC 2 cut(s) 799, 1679
FatI CATG 9 cut(s) 966, 989, 1104, 1212, 1615, 1679, 1687, 1821, 1838
FauI CCCGC 2 cut(s) 89, 614
FblI GTMKAC 2 cut(s) 63, 240
Fnu4HI GCNGC 7 cut(s) 26, 85, 323, 609, 874, 1247, 1350
FokI GGATG 5 cut(s) 323, 499, 716, 1076, 1358
FriOI GRGCYC 1 cut(s) 261
Fsp4HI GCNGC 7 cut(s) 26, 85, 323, 609, 874, 1247, 1350
FspBI CTAG 8 cut(s) 123, 185, 284, 480, 831, 981, 1274, 1722
GlaI GCGC 5 cut(s) 24, 448, 502, 1186, 1904
GluI GCNGC 7 cut(s) 26, 85, 323, 609, 874, 1247, 1350
HaeII RGCGCY 3 cut(s) 450, 504, 1906
HaeIII GGCC 5 cut(s) 915, 1384, 1643, 1743, 1844
HapII CCGG 2 cut(s) 168, 818
HgaI GACGC 4 cut(s) 102, 211, 415, 865
HhaI GCGC 5 cut(s) 25, 449, 503, 1187, 1905
Hin1I GRCGYC 5 cut(s) 94, 212, 222, 426, 876
Hin1II CATG 9 cut(s) 970, 993, 1108, 1216, 1619, 1683, 1691, 1825, 1842
Hin6I GCGC 5 cut(s) 23, 447, 501, 1185, 1903
HinP1I GCGC 5 cut(s) 23, 447, 501, 1185, 1903
HincII GTYRAC 4 cut(s) 241, 718, 1084, 1593
HindII GTYRAC 4 cut(s) 241, 718, 1084, 1593
HinfI GANTC 5 cut(s) 339, 757, 823, 1410, 1418
HpaI GTTAAC 1 cut(s) 1084
HpaII CCGG 2 cut(s) 168, 818
HphI GGTGA 7 cut(s) 542, 557, 598, 781, 832, 1214, 1486
Hpy188III TCNNGA 9 cut(s) 430, 750, 1220, 1295, 1535, 1732, 1778, 1794, 1887
Hpy99I CGWCG 8 cut(s) 96, 195, 217, 227, 242, 307, 431, 701
HpyCH4III ACNGT 6 cut(s) 61, 542, 790, 1456, 1568, 1811
HpyCH4IV ACGT 6 cut(s) 212, 305, 452, 534, 1574, 1754
HpyCH4V TGCA 7 cut(s) 199, 408, 734, 966, 1232, 1249, 1679
HpyF10VI GCNNNNNNNGC 6 cut(s) 93, 365, 432, 446, 500, 1255
HpyF3I CTNAG 5 cut(s) 507, 660, 1221, 1287, 1645
HpySE526I ACGT 6 cut(s) 212, 305, 452, 534, 1574, 1754
Hsp92I GRCGYC 5 cut(s) 94, 212, 222, 426, 876
Hsp92II CATG 9 cut(s) 970, 993, 1108, 1216, 1619, 1683, 1691, 1825, 1842
HspAI GCGC 5 cut(s) 23, 447, 501, 1185, 1903
KspAI GTTAAC 1 cut(s) 1084
Kzo9I GATC 6 cut(s) 386, 1066, 1302, 1734, 1780, 1873
LguI GCTCTTC 1 cut(s) 155
LmnI GCTCC 5 cut(s) 131, 432, 1150, 1405, 1831
Lsp1109I GCAGC 2 cut(s) 1233, 1336
LweI GCATC 4 cut(s) 556, 1258, 1336, 1492
MaeI CTAG 8 cut(s) 123, 185, 284, 480, 831, 981, 1274, 1722
MaeII ACGT 6 cut(s) 212, 305, 452, 534, 1574, 1754
MaeIII GTNAC 8 cut(s) 530, 769, 811, 956, 1141, 1276, 1558, 1658
MalI GATC 6 cut(s) 388, 1068, 1304, 1736, 1782, 1875
MboI GATC 6 cut(s) 386, 1066, 1302, 1734, 1780, 1873
MboII GAAGA 6 cut(s) 142, 145, 334, 787, 1069, 1505
MfeI CAATTG 3 cut(s) 349, 403, 1374
MhlI GDGCHC 2 cut(s) 261, 1611
MluCI AATT 6 cut(s) 349, 403, 410, 882, 1077, 1374
MmeI TCCRAC 5 cut(s) 115, 657, 995, 1018, 1853
MroXI GAANNNNTTC 1 cut(s) 1773
MseI TTAA 6 cut(s) 836, 1083, 1116, 1157, 1227, 1626
MslI CAYNNNNRTG 4 cut(s) 818, 1109, 1620, 1837
MspI CCGG 2 cut(s) 168, 818
MspR9I CCNGG 4 cut(s) 1137, 1177, 1612, 1712
MunI CAATTG 3 cut(s) 349, 403, 1374
MvaI CCWGG 4 cut(s) 1137, 1177, 1612, 1712
MvnI CGCG 1 cut(s) 25
MwoI GCNNNNNNNGC 6 cut(s) 93, 365, 432, 446, 500, 1255
NdeII GATC 6 cut(s) 386, 1066, 1302, 1734, 1780, 1873
NheI GCTAGC 1 cut(s) 830
NlaIII CATG 9 cut(s) 970, 993, 1108, 1216, 1619, 1683, 1691, 1825, 1842
NlaIV GGNNCC 3 cut(s) 172, 434, 1827
NmeAIII GCCGAG 2 cut(s) 385, 461
NmuCI GTSAC 5 cut(s) 530, 769, 811, 956, 1276
NspI RCATGY 4 cut(s) 993, 1619, 1683, 1691
OliI CACNNNNGTG 1 cut(s) 818
PaeI GCATGC 1 cut(s) 1619
PceI AGGCCT 1 cut(s) 1643
PciSI GCTCTTC 1 cut(s) 155
PcsI WCGNNNNNNNCGW 1 cut(s) 237
PdmI GAANNNNTTC 1 cut(s) 1773
PfeI GAWTC 5 cut(s) 339, 757, 823, 1410, 1418
PflFI GACNNNGTC 2 cut(s) 236, 898
PflMI CCANNNNNTGG 1 cut(s) 1391
PkrI GCNGC 7 cut(s) 27, 86, 324, 610, 875, 1248, 1351
PmaCI CACGTG 1 cut(s) 1755
PmlI CACGTG 1 cut(s) 1755
Ppu21I YACGTR 1 cut(s) 1755
PpuMI RGGWCCY 2 cut(s) 265, 743
PshBI ATTAAT 1 cut(s) 1227
PsiI TTATAA 1 cut(s) 1512
Psp5II RGGWCCY 2 cut(s) 265, 743
Psp6I CCWGG 4 cut(s) 1135, 1175, 1610, 1710
PspCI CACGTG 1 cut(s) 1755
PspGI CCWGG 4 cut(s) 1135, 1175, 1610, 1710
PspN4I GGNNCC 3 cut(s) 172, 434, 1827
PspPI GGNCC 3 cut(s) 265, 743, 1842
PspPPI RGGWCCY 2 cut(s) 265, 743
PsrI GAACNNNNNNTAC 2 cut(s) 1564, 1596
PsyI GACNNNGTC 2 cut(s) 236, 898
RsaI GTAC 1 cut(s) 1880
RsaNI GTAC 1 cut(s) 1879
RseI CAYNNNNRTG 4 cut(s) 818, 1109, 1620, 1837
SalI GTCGAC 1 cut(s) 239
SapI GCTCTTC 1 cut(s) 155
SaqAI TTAA 6 cut(s) 836, 1083, 1116, 1157, 1227, 1626
SatI GCNGC 7 cut(s) 26, 85, 323, 609, 874, 1247, 1350
Sau3AI GATC 6 cut(s) 386, 1066, 1302, 1734, 1780, 1873
Sau96I GGNCC 3 cut(s) 265, 743, 1842
ScrFI CCNGG 4 cut(s) 1137, 1177, 1612, 1712
SduI GDGCHC 2 cut(s) 261, 1611
SfaNI GCATC 4 cut(s) 556, 1258, 1336, 1492
SinI GGWCC 2 cut(s) 265, 743
SmiMI CAYNNNNRTG 4 cut(s) 818, 1109, 1620, 1837
SmlI CTYRAG 3 cut(s) 98, 722, 1433
SmoI CTYRAG 3 cut(s) 98, 722, 1433
SphI GCATGC 1 cut(s) 1619
Sse9I AATT 6 cut(s) 349, 403, 410, 882, 1077, 1374
SseBI AGGCCT 1 cut(s) 1643
SspMI CTAG 8 cut(s) 123, 185, 284, 480, 831, 981, 1274, 1722
StuI AGGCCT 1 cut(s) 1643
StyD4I CCNGG 4 cut(s) 1135, 1175, 1610, 1710
StyI CCWWGG 1 cut(s) 1525
TaaI ACNGT 6 cut(s) 61, 542, 790, 1456, 1568, 1811
TaiI ACGT 6 cut(s) 215, 308, 455, 537, 1577, 1757
TaqI TCGA 6 cut(s) 215, 231, 240, 302, 1252, 1888
TasI AATT 6 cut(s) 349, 403, 410, 882, 1077, 1374
TauI GCSGC 5 cut(s) 28, 87, 325, 611, 876
TfiI GAWTC 5 cut(s) 339, 757, 823, 1410, 1418
Tru1I TTAA 6 cut(s) 836, 1083, 1116, 1157, 1227, 1626
Tru9I TTAA 6 cut(s) 836, 1083, 1116, 1157, 1227, 1626
TscAI CASTG 4 cut(s) 768, 795, 1058, 1751
TseFI GTSAC 5 cut(s) 530, 769, 811, 956, 1276
TseI GCWGC 2 cut(s) 1246, 1349
Tsp45I GTSAC 5 cut(s) 530, 769, 811, 956, 1276
TspDTI ATGAA 8 cut(s) 663, 1121, 1139, 1402, 1686, 1782, 1827, 1875
TspGWI ACGGA 2 cut(s) 1059, 1866
TspRI CASTG 4 cut(s) 768, 795, 1058, 1751
Tth111I GACNNNGTC 2 cut(s) 236, 898
Van91I CCANNNNNTGG 1 cut(s) 1391
VpaK11BI GGWCC 2 cut(s) 265, 743
VspI ATTAAT 1 cut(s) 1227
XagI CCTNNNNNAGG 2 cut(s) 138, 272
XapI RAATTY 2 cut(s) 410, 882
XceI RCATGY 4 cut(s) 993, 1619, 1683, 1691
XcmI CCANNNNNNNNNTGG 1 cut(s) 1836
XmiI GTMKAC 2 cut(s) 63, 240
XmnI GAANNNNTTC 1 cut(s) 1773
XspI CTAG 8 cut(s) 123, 185, 284, 480, 831, 981, 1274, 1722
ZraI GACGTC 1 cut(s) 213
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.