RchiOBHm_Chr6g0301031

S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0013169
Physical Location & Seq
Reverse (-)
61604025 .. 61609077
5053 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ27034

Sequence Viewer

Length: 1947 bp
ATGTCCACATCCTCAGCCCGAGCACGGCTGGCTCTCATAGCCCTCCTCTCCGCCACCACGTTCTACTGTTTCTACAAATCTCGCCGCCTCAAGCAACTCTCCCTAAACCCTAACCCTAGCTCCTCTCCGCCTCGCAAAGGCAAGCTCTTCTTCATTTCCCAAACCGGCACCTCCAAAGCCCTAGCTCAGCGTCTTCTAAAACTTCTAGCCTCCAATGGCCTCGCCTTCGAGCTCGTCGACCCTAGCAGCTACGAGCCAGAGGACCTCCCCAAGGAAACCCTAGTCCTGATCGTCGCTTCCACCTGGGACGATGGACAACCGCCTCCGAGCGCCAAGTTCTTCACCAATTGGCTCGCGGAGAGCGCCGAGGACTTCAGAGTGGGGTCCATGCTGCTCTCGCAGTGCAGATTCTCGGTGTTCGGCGTCGGAAGCCGAGCCTACGGTGCGTCGTTCAATGCGGTGGGGAGGGACTTGTCGAAGTGGATGAGGGCGTTGGGCGCCGCCGAGATATTGCCGGTTTGGGAAGGTGACGTGGACGACGGGGATATAGACCAGGTCTTTGAGGCTTGGAGTGGTAAGCTGATGAGGTTTTTGAAGGCCGGTGTGGCCGAAAACGGTGTCGCTTTGGGTAATGGTGTTGGTGTTGATGCTGAAGGTGAAGCTGAGTATTCTGACGTGTCAGATTATGATGAGGAGGAGGAGGAGGAGGAGACTGTGGTTGAGTCTGGGGTTGTTGATCTTGAGGACATTGCAGGTAAAGCACCTTCAAGGGGGACGGTGACGGTGGCTAAGACTAAGACCAATGGGATAGTGAATGGTAAAAGAGATATGGTCACTCCGGTGATTCGTGCCAGCTTAGAGAAGCAGGGGTACAAAATTATTGGTTCGCATAGTGGTGTCAAAATTTGTAGATGGACAAAGTCACAGCTTAGAGGCCGAGGTGGTTGCTATAAACACTCGTTTTATGGCATAGAAAGTCATAGGTGCATGGAGGCAACCCCTAGTTTGGCATGTGCCAATAAATGTGTTTTCTGTTGGAGACATCATACAAACCCTGTTGGAAAGAGTTGGCAGTGGAAGATGGACGATCCATTAGAGATTGTGAAAACTGCCATAGACCTGCACAGTAAAATGATTAAGCAAATGAAAGGAGTTCCTGGAGTTACATTGGAGCGATTAACAGAGGGTCTCTCTCCCAGGCATTGTGCCTTGTCACTTGTTGGTGAACCTATTATGTATCCAGAGATTAATACACTAGTAGATGAGCTGCATTGTAGACGGATTTCTACTTTTCTAGTGACAAATGCTCAATTTCCTGAAAAAATTAGAATGCTGAAACCTATTACACAGTTATATGTAAGTGTGGATGCTGCAACTAAAGAGAGTTTGAAGGCAATTGACAGGCCACTTTTTGGAGATTTTTGGGAGCGATTTATTGATTCCTTGAAAGCTCTCAAGGAAAAACAGCAGCGAACTGTTTATCGCTTAACTTTGGTCAAGGGATGGAATACAGAAGAAATTGAGGCTTATTATAACATCTTTAGCATTGGTAACCCCGATTTTGTTGAAATCAAAGGAGTTACATATTGTGGATCGTCTGCTACCTCAAAATTGACGATGGAGAATGTGCCCTGGCATGCTGATGTTAAAGCTTTTTCAGAGGCCTTGGCTTTGAGAAGTAATGGCGAGTATGAGGTCGCATGTGAGCATGTCCACTCATGTTGTGTCCTCCTGGCAAAAACTAAAAAGTTCAAGATCAATGGGCAGTGGTTCACATGGATAGACTATGAAAAGTTTCATGATCTGGTGGCTTCTGGAAAACCCTTTGACAGTAATGATTATATGGCTGCCACACCTTCATGGGCTGTTTATGGGGCAGTTGAAGGTGGATTTGATCCCTATCAATCTCGATATAGGAAGGAGCGACATCACAGATCAAACCAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Flavodoxin_1 Radical_SAM Wyosine_form

Protein Analysis

648

Amino Acids

72.32

Weight (kDa)

6.92

Isoelectric Point (pI)

35.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Flavodoxin_1 PF00258 49 - 190 2.1e-26 Flavodoxin
Radical_SAM PF04055 336 - 511 3.3e-16 Radical SAM superfamily
Wyosine_form PF08608 517 - 580 9.3e-24 Wyosine base formation
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013169)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G75200
fragaria_vesca FvH4_2g39810 FvH4_2g39810
malus_domestica MD08G1031900.v1.1 MD15G1027800.v1.1
prunus_persica Prupe.1G379500_v2.0.a1 Prupe.1G379500_v2.0.a1
pyrus_communis pycom08g02520 pycom15g02380
rosa_chinensis RchiOBHm_Chr6g0301031
rosa_laevigata RLG00000011283
rosa_multiflora Rmu_co8483439.1_g000001
rosa_roxburghii Rroxscaffold_7G00167240
rosa_rugosa Rorug06G0300100
rosa_samantha Rh6AG414000 Rh6BG421000 Rh6CG428600 Rh6DG414000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1533
Acc36I ACCTGC 2 cut(s) 743, 1128
AccB1I GGYRCC 2 cut(s) 167, 497
AccB7I CCANNNNNTGG 1 cut(s) 1412
AccI GTMKAC 2 cut(s) 237, 1276
AccII CGCG 1 cut(s) 356
AciI CCGC 7 cut(s) 51, 85, 128, 320, 356, 458, 501
AclWI GGATC 3 cut(s) 1082, 1600, 1889
AcoI YGGCCR 1 cut(s) 606
AcsI RAATTY 1 cut(s) 903
AcuI CTGAAG 2 cut(s) 358, 672
AcyI GRCGYC 2 cut(s) 423, 498
AfaI GTAC 1 cut(s) 872
AfiI CCNNNNNNNGG 6 cut(s) 24, 137, 271, 770, 1006, 1412
AflIII ACRYGT 1 cut(s) 675
AgsI TTSAA 8 cut(s) 454, 595, 768, 1390, 1447, 1568, 1753, 1883
AhlI ACTAGT 1 cut(s) 1255
AjiI CACGTC 2 cut(s) 532, 676
AjnI CCWGG 6 cut(s) 302, 552, 1156, 1196, 1631, 1731
AleI CACNNNNGTG 1 cut(s) 839
Alw21I GWGCWC 2 cut(s) 25, 234
Alw26I GTCTC 3 cut(s) 704, 1033, 1193
AlwI GGATC 3 cut(s) 1082, 1600, 1889
Ama87I CYCGRG 1 cut(s) 18
AoxI GGCC 6 cut(s) 217, 597, 606, 934, 1403, 1662
ApeKI GCWGC 6 cut(s) 246, 391, 1267, 1370, 1468, 1847
ApoI RAATTY 1 cut(s) 903
ArsI GACNNNNNNTTYG 4 cut(s) 542, 574, 603, 635
AseI ATTAAT 1 cut(s) 1248
Asp700I GAANNNNTTC 1 cut(s) 1794
AspLEI GCGC 3 cut(s) 332, 365, 500
AspS9I GGNCC 2 cut(s) 262, 384
AsuHPI GGTGA 6 cut(s) 334, 539, 668, 790, 853, 1235
AvaI CYCGRG 1 cut(s) 18
AvaII GGWCC 2 cut(s) 262, 384
BaeGI GKGCMC 1 cut(s) 1632
BanI GGYRCC 2 cut(s) 167, 497
BanII GRGCYC 1 cut(s) 234
BarI GAAGNNNNNNTAC 4 cut(s) 748, 780, 854, 886
BbsI GAAGAC 1 cut(s) 185
Bbv12I GWGCWC 2 cut(s) 25, 234
BbvCI CCTCAGC 1 cut(s) 13
BbvI GCAGC 6 cut(s) 258, 378, 1254, 1357, 1480, 1834
BccI CCATC 5 cut(s) 305, 906, 1075, 1497, 1612
BceAI ACGGC 1 cut(s) 41
BcgI CGANNNNNNTGC 2 cut(s) 927, 961
BciT130I CCWGG 6 cut(s) 304, 554, 1158, 1198, 1633, 1733
BciVI GTATCC 1 cut(s) 1248
BcoDI GTCTC 3 cut(s) 704, 1033, 1193
BcuI ACTAGT 1 cut(s) 1255
BfaI CTAG 8 cut(s) 117, 182, 206, 243, 281, 1002, 1256, 1295
BfoI RGCGCY 3 cut(s) 333, 366, 501
BfuAI ACCTGC 2 cut(s) 743, 1128
BfuI GTATCC 1 cut(s) 1248
BglI GCCNNNNNGGC 1 cut(s) 605
BisI GCNGC 8 cut(s) 85, 247, 392, 501, 1268, 1371, 1469, 1848
BlpI GCTNAGC 1 cut(s) 186
BlsI GCNGC 8 cut(s) 86, 248, 393, 502, 1269, 1372, 1470, 1849
Bme1390I CCNGG 6 cut(s) 304, 554, 1158, 1198, 1633, 1733
Bme18I GGWCC 2 cut(s) 262, 384
BmeT110I CYCGRG 1 cut(s) 18
BmgBI CACGTC 2 cut(s) 532, 676
BmgT120I GGNCC 2 cut(s) 262, 384
BmiI GGNNCC 3 cut(s) 169, 385, 499
BmrFI CCNGG 6 cut(s) 304, 554, 1158, 1198, 1633, 1733
BmsI GCATC 2 cut(s) 637, 1357
BpiI GAAGAC 1 cut(s) 185
BplI GAGNNNNNCTC 2 cut(s) 1175, 1207
BpmI CTGGAG 1 cut(s) 1179
Bpu10I CCTNAGC 1 cut(s) 13
Bpu1102I GCTNAGC 1 cut(s) 186
BpuEI CTTGAG 3 cut(s) 74, 761, 1439
BsaBI GATNNNNATC 1 cut(s) 1899
BsaHI GRCGYC 2 cut(s) 423, 498
BsaI GGTCTC 1 cut(s) 1193
BsaJI CCNNGG 7 cut(s) 270, 303, 366, 937, 1196, 1631, 1665
BsaWI WCCGGW 1 cut(s) 838
BsaXI ACNNNNNCTCC 2 cut(s) 104, 134
Bsc4I CCNNNNNNNGG 6 cut(s) 24, 137, 271, 770, 1006, 1412
Bse118I RCCGGY 3 cut(s) 164, 514, 599
Bse1I ACTGG 1 cut(s) 1942
Bse3DI GCAATG 1 cut(s) 747
Bse8I GATNNNNATC 1 cut(s) 1899
BseBI CCWGG 6 cut(s) 304, 554, 1158, 1198, 1633, 1733
BseDI CCNNGG 7 cut(s) 270, 303, 366, 937, 1196, 1631, 1665
BseGI GGATG 4 cut(s) 8, 489, 1372, 1508
BseJI GATNNNNATC 1 cut(s) 1899
BseLI CCNNNNNNNGG 6 cut(s) 24, 137, 271, 770, 1006, 1412
BseMI GCAATG 1 cut(s) 747
BseMII CTCAG 3 cut(s) 27, 200, 654
BseNI ACTGG 1 cut(s) 1942
BseRI GAGGAG 8 cut(s) 35, 112, 707, 710, 713, 716, 719, 722
BseSI GKGCMC 1 cut(s) 1632
BseXI GCAGC 6 cut(s) 258, 378, 1254, 1357, 1480, 1834
BsgI GTGCAG 2 cut(s) 424, 1106
Bsh1236I CGCG 1 cut(s) 356
BshFI GGCC 6 cut(s) 219, 599, 608, 936, 1405, 1664
BshNI GGYRCC 2 cut(s) 167, 497
BsiHKAI GWGCWC 2 cut(s) 25, 234
BsiHKCI CYCGRG 1 cut(s) 18
BsiSI CCGG 4 cut(s) 165, 515, 600, 839
BslFI GGGAC 3 cut(s) 320, 482, 787
BslI CCNNNNNNNGG 6 cut(s) 24, 137, 271, 770, 1006, 1412
BsmAI GTCTC 3 cut(s) 704, 1033, 1193
BsmFI GGGAC 3 cut(s) 320, 482, 787
BsmI GAATGC 1 cut(s) 1335
BsnI GGCC 6 cut(s) 219, 599, 608, 936, 1405, 1664
Bso31I GGTCTC 1 cut(s) 1193
BsoBI CYCGRG 1 cut(s) 18
Bsp1286I GDGCHC 3 cut(s) 25, 234, 1632
Bsp143I GATC 8 cut(s) 288, 736, 1087, 1592, 1755, 1801, 1894, 1934
Bsp1720I GCTNAGC 1 cut(s) 186
BspACI CCGC 7 cut(s) 51, 85, 128, 320, 356, 458, 501
BspANI GGCC 6 cut(s) 219, 599, 608, 936, 1405, 1664
BspCNI CTCAG 3 cut(s) 26, 199, 655
BspFNI CGCG 1 cut(s) 356
BspHI TCATGA 1 cut(s) 1798
BspLI GGNNCC 3 cut(s) 169, 385, 499
BspMI ACCTGC 2 cut(s) 743, 1128
BspPI GGATC 3 cut(s) 1082, 1600, 1889
BspQI GCTCTTC 1 cut(s) 152
BspT107I GGYRCC 2 cut(s) 167, 497
BspTNI GGTCTC 1 cut(s) 1193
BsrDI GCAATG 1 cut(s) 747
BsrFI RCCGGY 3 cut(s) 164, 514, 599
BsrI ACTGG 1 cut(s) 1942
BssAI RCCGGY 3 cut(s) 164, 514, 599
BssECI CCNNGG 7 cut(s) 270, 303, 366, 937, 1196, 1631, 1665
BssMI GATC 8 cut(s) 288, 736, 1087, 1592, 1755, 1801, 1894, 1934
BssNI GRCGYC 2 cut(s) 423, 498
BssT1I CCWWGG 2 cut(s) 270, 1665
Bst2UI CCWGG 6 cut(s) 304, 554, 1158, 1198, 1633, 1733
Bst6I CTCTTC 1 cut(s) 152
BstACI GRCGYC 2 cut(s) 423, 498
BstC8I GCNNGC 5 cut(s) 30, 143, 354, 853, 1638
BstDEI CTNAG 7 cut(s) 13, 186, 663, 789, 795, 856, 929
BstEII GGTNACC 1 cut(s) 1550
BstENI CCTNNNNNAGG 2 cut(s) 135, 269
BstF5I GGATG 4 cut(s) 8, 489, 1372, 1508
BstFNI CGCG 1 cut(s) 356
BstH2I RGCGCY 3 cut(s) 333, 366, 501
BstHHI GCGC 3 cut(s) 332, 365, 500
BstKTI GATC 8 cut(s) 291, 739, 1090, 1595, 1758, 1804, 1897, 1937
BstMAI GTCTC 3 cut(s) 704, 1033, 1193
BstMBI GATC 8 cut(s) 288, 736, 1087, 1592, 1755, 1801, 1894, 1934
BstMWI GCNNNNNNNGC 9 cut(s) 29, 38, 362, 397, 429, 443, 497, 605, 758
BstNI CCWGG 6 cut(s) 304, 554, 1158, 1198, 1633, 1733
BstNSI RCATGY 4 cut(s) 1014, 1640, 1704, 1712
BstPI GGTNACC 1 cut(s) 1550
BstSCI CCNGG 6 cut(s) 302, 552, 1156, 1196, 1631, 1731
BstSLI GKGCMC 1 cut(s) 1632
BstUI CGCG 1 cut(s) 356
BstV1I GCAGC 6 cut(s) 258, 378, 1254, 1357, 1480, 1834
BstV2I GAAGAC 1 cut(s) 185
BsuI GTATCC 1 cut(s) 1248
BsuRI GGCC 6 cut(s) 219, 599, 608, 936, 1405, 1664
BtrI CACGTC 2 cut(s) 532, 676
BtsCI GGATG 4 cut(s) 8, 489, 1372, 1508
BtsI GCAGTG 3 cut(s) 407, 1079, 1772
BtsIMutI CAGTG 3 cut(s) 407, 1079, 1772
BveI ACCTGC 2 cut(s) 743, 1128
Cac8I GCNNGC 5 cut(s) 30, 143, 354, 853, 1638
CciI TCATGA 1 cut(s) 1798
CfoI GCGC 3 cut(s) 332, 365, 500
Cfr10I RCCGGY 3 cut(s) 164, 514, 599
Cfr13I GGNCC 2 cut(s) 262, 384
CseI GACGC 3 cut(s) 179, 412, 435
CsiI ACCWGGT 1 cut(s) 552
Csp6I GTAC 1 cut(s) 871
CspCI CAANNNNNGTGG 2 cut(s) 1703, 1738
CviQI GTAC 1 cut(s) 871
DdeI CTNAG 7 cut(s) 13, 186, 663, 789, 795, 856, 929
DinI GGCGCC 1 cut(s) 499
DpnI GATC 8 cut(s) 290, 738, 1089, 1594, 1757, 1803, 1896, 1936
DpnII GATC 8 cut(s) 288, 736, 1087, 1592, 1755, 1801, 1894, 1934
EaeI YGGCCR 1 cut(s) 606
Eam1104I CTCTTC 1 cut(s) 152
EarI CTCTTC 1 cut(s) 152
EciI GGCGGA 2 cut(s) 40, 117
Ecl136II GAGCTC 1 cut(s) 232
Eco130I CCWWGG 2 cut(s) 270, 1665
Eco147I AGGCCT 1 cut(s) 1664
Eco24I GRGCYC 1 cut(s) 234
Eco31I GGTCTC 1 cut(s) 1193
Eco47I GGWCC 2 cut(s) 262, 384
Eco53kI GAGCTC 1 cut(s) 232
Eco57I CTGAAG 2 cut(s) 358, 672
Eco88I CYCGRG 1 cut(s) 18
Eco91I GGTNACC 1 cut(s) 1550
EcoICRI GAGCTC 1 cut(s) 232
EcoNI CCTNNNNNAGG 2 cut(s) 135, 269
EcoO109I RGGNCCY 1 cut(s) 262
EcoO65I GGTNACC 1 cut(s) 1550
EcoRII CCWGG 6 cut(s) 302, 552, 1156, 1196, 1631, 1731
EcoT14I CCWWGG 2 cut(s) 270, 1665
EcoT38I GRGCYC 1 cut(s) 234
EgeI GGCGCC 1 cut(s) 499
EheI GGCGCC 1 cut(s) 499
ErhI CCWWGG 2 cut(s) 270, 1665
FalI AAGNNNNNCTT 2 cut(s) 134, 166
FaqI GGGAC 3 cut(s) 320, 482, 787
FblI GTMKAC 2 cut(s) 237, 1276
Fnu4HI GCNGC 8 cut(s) 85, 247, 392, 501, 1268, 1371, 1469, 1848
FokI GGATG 3 cut(s) 496, 1379, 1515
FriOI GRGCYC 1 cut(s) 234
Fsp4HI GCNGC 8 cut(s) 85, 247, 392, 501, 1268, 1371, 1469, 1848
FspBI CTAG 8 cut(s) 117, 182, 206, 243, 281, 1002, 1256, 1295
GlaI GCGC 3 cut(s) 331, 364, 499
GluI GCNGC 8 cut(s) 85, 247, 392, 501, 1268, 1371, 1469, 1848
GsuI CTGGAG 1 cut(s) 1179
HaeII RGCGCY 3 cut(s) 333, 366, 501
HaeIII GGCC 6 cut(s) 219, 599, 608, 936, 1405, 1664
HapII CCGG 4 cut(s) 165, 515, 600, 839
HgaI GACGC 3 cut(s) 179, 412, 435
HhaI GCGC 3 cut(s) 332, 365, 500
Hin1I GRCGYC 2 cut(s) 423, 498
Hin6I GCGC 3 cut(s) 330, 363, 498
HinP1I GCGC 3 cut(s) 330, 363, 498
HincII GTYRAC 1 cut(s) 238
HindII GTYRAC 1 cut(s) 238
HindIII AAGCTT 1 cut(s) 1650
HinfI GANTC 4 cut(s) 408, 722, 844, 1439
HpaII CCGG 4 cut(s) 165, 515, 600, 839
HphI GGTGA 6 cut(s) 334, 539, 668, 790, 853, 1235
Hpy166II GTNNAC 7 cut(s) 6, 238, 535, 1226, 1277, 1714, 1773
Hpy188I TCNGA 6 cut(s) 327, 377, 428, 673, 682, 1660
Hpy188III TCNNGA 8 cut(s) 286, 740, 1241, 1316, 1753, 1799, 1815, 1908
Hpy8I GTNNAC 7 cut(s) 6, 238, 535, 1226, 1277, 1714, 1773
Hpy99I CGWCG 5 cut(s) 239, 296, 428, 451, 542
HpyAV CCTTC 9 cut(s) 235, 518, 589, 647, 774, 1384, 1866, 1877, 1912
HpyCH4IV ACGT 3 cut(s) 59, 531, 675
HpyCH4V TGCA 6 cut(s) 405, 752, 987, 1123, 1270, 1373
HpyF10VI GCNNNNNNNGC 9 cut(s) 29, 38, 362, 397, 429, 443, 497, 605, 758
HpyF3I CTNAG 7 cut(s) 13, 186, 663, 789, 795, 856, 929
HpySE526I ACGT 3 cut(s) 59, 531, 675
Hsp92I GRCGYC 2 cut(s) 423, 498
HspAI GCGC 3 cut(s) 330, 363, 498
KasI GGCGCC 1 cut(s) 497
Kzo9I GATC 8 cut(s) 288, 736, 1087, 1592, 1755, 1801, 1894, 1934
LguI GCTCTTC 1 cut(s) 152
LmnI GCTCC 4 cut(s) 125, 1171, 1426, 1921
Lsp1109I GCAGC 6 cut(s) 258, 378, 1254, 1357, 1480, 1834
LweI GCATC 2 cut(s) 637, 1357
MabI ACCWGGT 1 cut(s) 552
MaeI CTAG 8 cut(s) 117, 182, 206, 243, 281, 1002, 1256, 1295
MaeII ACGT 3 cut(s) 59, 531, 675
MaeIII GTNAC 9 cut(s) 527, 778, 832, 921, 1162, 1212, 1297, 1550, 1579
MalI GATC 8 cut(s) 290, 738, 1089, 1594, 1757, 1803, 1896, 1936
MboI GATC 8 cut(s) 288, 736, 1087, 1592, 1755, 1801, 1894, 1934
MboII GAAGA 6 cut(s) 139, 142, 185, 331, 1090, 1526
MfeI CAATTG 2 cut(s) 346, 1395
MhlI GDGCHC 3 cut(s) 25, 234, 1632
MluCI AATT 8 cut(s) 346, 876, 903, 1310, 1323, 1395, 1518, 1610
Mly113I GGCGCC 1 cut(s) 498
MlyI GAGTC 1 cut(s) 731
MmeI TCCRAC 3 cut(s) 406, 1016, 1039
MroXI GAANNNNTTC 1 cut(s) 1794
MseI TTAA 5 cut(s) 1137, 1178, 1248, 1487, 1647
MslI CAYNNNNRTG 4 cut(s) 839, 894, 1641, 1858
MspI CCGG 4 cut(s) 165, 515, 600, 839
MspR9I CCNGG 6 cut(s) 304, 554, 1158, 1198, 1633, 1733
MunI CAATTG 2 cut(s) 346, 1395
Mva1269I GAATGC 1 cut(s) 1335
MvaI CCWGG 6 cut(s) 304, 554, 1158, 1198, 1633, 1733
MvnI CGCG 1 cut(s) 356
MwoI GCNNNNNNNGC 9 cut(s) 29, 38, 362, 397, 429, 443, 497, 605, 758
NarI GGCGCC 1 cut(s) 498
NdeII GATC 8 cut(s) 288, 736, 1087, 1592, 1755, 1801, 1894, 1934
NlaIV GGNNCC 3 cut(s) 169, 385, 499
NmeAIII GCCGAG 4 cut(s) 391, 458, 529, 962
NmuCI GTSAC 6 cut(s) 527, 778, 832, 921, 1212, 1297
NspI RCATGY 4 cut(s) 1014, 1640, 1704, 1712
OliI CACNNNNGTG 1 cut(s) 839
PaeI GCATGC 1 cut(s) 1640
PagI TCATGA 1 cut(s) 1798
PceI AGGCCT 1 cut(s) 1664
PciSI GCTCTTC 1 cut(s) 152
PcsI WCGNNNNNNNCGW 1 cut(s) 234
PctI GAATGC 1 cut(s) 1335
PdmI GAANNNNTTC 1 cut(s) 1794
PfeI GAWTC 3 cut(s) 408, 844, 1439
PflFI GACNNNGTC 2 cut(s) 554, 919
PflMI CCANNNNNTGG 1 cut(s) 1412
PfoI TCCNGGA 1 cut(s) 1156
PkrI GCNGC 8 cut(s) 86, 248, 393, 502, 1269, 1372, 1470, 1849
PleI GAGTC 1 cut(s) 730
PluTI GGCGCC 1 cut(s) 501
PpsI GAGTC 1 cut(s) 730
PpuMI RGGWCCY 1 cut(s) 262
PshBI ATTAAT 1 cut(s) 1248
PsiI TTATAA 1 cut(s) 1533
Psp124BI GAGCTC 1 cut(s) 234
Psp5II RGGWCCY 1 cut(s) 262
Psp6I CCWGG 6 cut(s) 302, 552, 1156, 1196, 1631, 1731
PspEI GGTNACC 1 cut(s) 1550
PspGI CCWGG 6 cut(s) 302, 552, 1156, 1196, 1631, 1731
PspN4I GGNNCC 3 cut(s) 169, 385, 499
PspPI GGNCC 2 cut(s) 262, 384
PspPPI RGGWCCY 1 cut(s) 262
PsyI GACNNNGTC 2 cut(s) 554, 919
RsaI GTAC 1 cut(s) 872
RsaNI GTAC 1 cut(s) 871
RseI CAYNNNNRTG 4 cut(s) 839, 894, 1641, 1858
SacI GAGCTC 1 cut(s) 234
SalI GTCGAC 1 cut(s) 236
SapI GCTCTTC 1 cut(s) 152
SaqAI TTAA 5 cut(s) 1137, 1178, 1248, 1487, 1647
SatI GCNGC 8 cut(s) 85, 247, 392, 501, 1268, 1371, 1469, 1848
Sau3AI GATC 8 cut(s) 288, 736, 1087, 1592, 1755, 1801, 1894, 1934
Sau96I GGNCC 2 cut(s) 262, 384
SchI GAGTC 1 cut(s) 731
ScrFI CCNGG 6 cut(s) 304, 554, 1158, 1198, 1633, 1733
SduI GDGCHC 3 cut(s) 25, 234, 1632
SexAI ACCWGGT 1 cut(s) 552
SfaNI GCATC 2 cut(s) 637, 1357
SfiI GGCCNNNNNGGCC 1 cut(s) 605
SfoI GGCGCC 1 cut(s) 499
SinI GGWCC 2 cut(s) 262, 384
SmiMI CAYNNNNRTG 4 cut(s) 839, 894, 1641, 1858
SmlI CTYRAG 3 cut(s) 89, 740, 1454
SmoI CTYRAG 3 cut(s) 89, 740, 1454
SpeI ACTAGT 1 cut(s) 1255
SphI GCATGC 1 cut(s) 1640
Sse9I AATT 8 cut(s) 346, 876, 903, 1310, 1323, 1395, 1518, 1610
SseBI AGGCCT 1 cut(s) 1664
SsiI CCGC 7 cut(s) 51, 85, 128, 320, 356, 458, 501
SspDI GGCGCC 1 cut(s) 497
SspMI CTAG 8 cut(s) 117, 182, 206, 243, 281, 1002, 1256, 1295
SstI GAGCTC 1 cut(s) 234
StuI AGGCCT 1 cut(s) 1664
StyD4I CCNGG 6 cut(s) 302, 552, 1156, 1196, 1631, 1731
StyI CCWWGG 2 cut(s) 270, 1665
TaiI ACGT 3 cut(s) 62, 534, 678
TaqI TCGA 4 cut(s) 228, 237, 476, 1909
TasI AATT 8 cut(s) 346, 876, 903, 1310, 1323, 1395, 1518, 1610
TauI GCSGC 2 cut(s) 87, 503
TfiI GAWTC 3 cut(s) 408, 844, 1439
Tru1I TTAA 5 cut(s) 1137, 1178, 1248, 1487, 1647
Tru9I TTAA 5 cut(s) 1137, 1178, 1248, 1487, 1647
TscAI CASTG 3 cut(s) 407, 1079, 1772
TseFI GTSAC 6 cut(s) 527, 778, 832, 921, 1212, 1297
TseI GCWGC 6 cut(s) 246, 391, 1267, 1370, 1468, 1847
Tsp45I GTSAC 6 cut(s) 527, 778, 832, 921, 1212, 1297
TspDTI ATGAA 5 cut(s) 142, 1160, 1787, 1803, 1848
TspGWI ACGGA 1 cut(s) 1294
TspRI CASTG 3 cut(s) 407, 1079, 1772
Tth111I GACNNNGTC 2 cut(s) 554, 919
Van91I CCANNNNNTGG 1 cut(s) 1412
VpaK11BI GGWCC 2 cut(s) 262, 384
VspI ATTAAT 1 cut(s) 1248
XagI CCTNNNNNAGG 2 cut(s) 135, 269
XapI RAATTY 1 cut(s) 903
XceI RCATGY 4 cut(s) 1014, 1640, 1704, 1712
XmiI GTMKAC 2 cut(s) 237, 1276
XmnI GAANNNNTTC 1 cut(s) 1794
XspI CTAG 8 cut(s) 117, 182, 206, 243, 281, 1002, 1256, 1295
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.