pycom15g02380

S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0013169
Physical Location & Seq
Forward (+)
1388872 .. 1391829
2958 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g02380.1

Sequence Viewer

Length: 1935 bp
ATGTTCCTCTCCTCCGCTCCTGCGCGGCTCACTCTCCTCGCCATCCTCTCCGCCACCACTGTCTACTGTTTCTACAAATCCCGCCGCCTCCGACGCCTCAAGCTCTCCCTAAACCCTAACCCTGGCTCTTCGCCTCGCAGAGGCAAGCTCTTCTTCGTCTCCCAAACCGGAACCTCCGAAGCCCTAGCTCGTCGCCTGCTCGATTTTCTGAAATTAAAAGGCGTCGCTTTCGACCTCGTCGACCCCAACAACTACGAGCCGGAAGACCTCCCGAAGGAAACCCTAGTTCTGATCGTCGCTTCGACTTGGGATGACGGGAAGCCGCCTCCGAACGCCAAGTTCTTCTCCGATTGGCTCGCCGAGAGCGCGGAGGACTTCAGGGTGGGATCGCTGCTGCTCTCCAATTGCAAATTCGCCGTCTTCGGCGTTGGGAGCCGAGCCTACGGCGCTACGTTCAATGCGGTGGGGAAGGACTTGGCGAGGAGGATGAGGGCGTTGGGCGCTTCTGAGATGTTTCCGATTTGGGAAGGTGACGTGGACGGTGGTGATTTAGACGAGGTGTTTGATGCTTGGAGTGGGAAGGTGGTGAGGTTTCTGAAGGGTGGTGCGGCGGAAAATGGCGGGGTTTTGGGTAATGGGGTTAGTGATGAATGTGAAGCTGAGAGCTTTGATGTTTCAGACGAGGAAGATGAGGAGGAGGAGGACGGTGGGGAGTCAGGTGTTGTTGATCTTGAGGATATTGCAGGTAAAGGGCCTTCAAGAAGGAACCCAGTGGCGGCGACGAAGACGAACGGGACAGTAAATGGTAAAAAAGAGATGGTCACTCCGGTGATTCGAGCTAGCTTAACGAAGCAGGGGTATAAAATTATTGGTTCTCATAGCGGTGTCAAAATTTGTAGATGGACAAAGTCGCAGCTTCGAGGCCGTGGAGGTTGCTACAAACACTCGTTTTATGGCATAGAAAGTCACAGGTGCATGGAGACAACCCCTAGTCTGGCATGTGCCAACAAATGTGTTTTTTGTTGGAGACATCACACAAATCCTGTCGGAAAGAGTTGGCAGTGGAAGATGGATGATCCGTTAGAGATTGTTAATTCTGCCATAGACCAGCACACGAAAATGATCAAGCAAATGAAAGGAGTTCCTGGTGTTACATTGGAGCGATTAACAGAGGGTCTCTCTCCCAGGCATTGTGCCTTATCACTTGTTGGTGAACCCATCATGTATCCTGAGATTAATGCACTCATAGATGAGCTGCATCGAAGACGGATTTCTACTTTTCTAGTAACAAATGCTCAGTTTCCTGAAAAGATCAAAATGCTGAACCCTATCACGCAGTTATATGTAAGTGTAGATGCTGCAACTAAAGAGAGTTTGAAGGCGATTGATAGGCCACTTTTTGGGGATTTTTGGGAGCGATTCATTGATTCCTTGACTTCTCTCAAGGACAAACAGCAACGAACTGTTTATCGCCTAACTCTGGTGAAGGGCTGGAATACAGAAGATATCGATGCTTATTATAACATTTTCAGCCTTGGCAGTCCTGATTTTGTTGAAATCAAGGGAGTTACATATTGTGGAACGTCTGCTACATCAAAGTTGACAATGGAGAATGTGCCCTGGCATGCTGATGTTAAAGCATTTTCAGAGGCCTTAGCTTTGAAAAGTAACAGGGAGTATGAAGTCGCATGTGAGCATGTCCACTCGTGCTGTGTCCTCCTGGCAAAAACTAGCAAGTTCAAGATTGATGGCCAGTGGTTCACGTGGATTGACTATGAAAAGTTTCACGATCTGGTGGCTTCTGGAAAACATTTTGACAGTAAGGATTACATGGCTCCAACACCTTCATGGGCCGTTTATGGGGCAGAGGAAGGCGGTTTTGATCCGTATCAGTCTCGATACAAGAAGGAGCGCCATCACAGAACAAACCAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Flavodoxin_1 Radical_SAM Wyosine_form

Protein Analysis

645

Amino Acids

71.99

Weight (kDa)

6.75

Isoelectric Point (pI)

38.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Flavodoxin_1 PF00258 50 - 191 3.1e-28 Flavodoxin
Radical_SAM PF04055 331 - 506 1.1e-14 Radical SAM superfamily
Wyosine_form PF08608 513 - 576 2.7e-23 Wyosine base formation
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013169)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G75200
fragaria_vesca FvH4_2g39810 FvH4_2g39810
malus_domestica MD08G1031900.v1.1 MD15G1027800.v1.1
prunus_persica Prupe.1G379500_v2.0.a1 Prupe.1G379500_v2.0.a1
pyrus_communis pycom08g02520 pycom15g02380
rosa_chinensis RchiOBHm_Chr6g0301031
rosa_laevigata RLG00000011283
rosa_multiflora Rmu_co8483439.1_g000001
rosa_roxburghii Rroxscaffold_7G00167240
rosa_rugosa Rorug06G0300100
rosa_samantha Rh6AG414000 Rh6BG421000 Rh6CG428600 Rh6DG414000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1521
Acc36I ACCTGC 1 cut(s) 734
AccB7I CCANNNNNTGG 1 cut(s) 1400
AccBSI CCGCTC 1 cut(s) 17
AccI GTMKAC 2 cut(s) 63, 240
AccII CGCG 2 cut(s) 25, 368
AclWI GGATC 3 cut(s) 394, 1070, 1877
AcoI YGGCCR 1 cut(s) 1750
AcsI RAATTY 2 cut(s) 410, 891
AcuI CTGAAG 2 cut(s) 361, 617
AcvI CACGTG 1 cut(s) 1764
AcyI GRCGYC 2 cut(s) 94, 222
AfiI CCNNNNNNNGG 8 cut(s) 122, 140, 274, 775, 994, 1400, 1480, 1860
AgsI TTSAA 6 cut(s) 457, 759, 1378, 1556, 1663, 1741
AjiI CACGTC 1 cut(s) 535
AjnI CCWGG 5 cut(s) 121, 1144, 1184, 1619, 1719
AjuI GAANNNNNNNTTGG 2 cut(s) 395, 427
AleI CACNNNNGTG 1 cut(s) 827
Alw26I GTCTC 5 cut(s) 163, 974, 1021, 1181, 1899
AlwI GGATC 3 cut(s) 394, 1070, 1877
AoxI GGCC 6 cut(s) 752, 922, 1391, 1650, 1750, 1851
ApeKI GCWGC 5 cut(s) 391, 394, 913, 1255, 1358
ApoI RAATTY 2 cut(s) 410, 891
ArsI GACNNNNNNTTYG 4 cut(s) 402, 434, 545, 577
AseI ATTAAT 1 cut(s) 1236
Asp700I GAANNNNTTC 1 cut(s) 1782
AspLEI GCGC 5 cut(s) 25, 368, 449, 503, 1914
AspS9I GGNCC 2 cut(s) 752, 1851
AsuHPI GGTGA 6 cut(s) 542, 557, 598, 841, 1223, 1495
AsuNHI GCTAGC 1 cut(s) 839
BaeGI GKGCMC 1 cut(s) 1620
BalI TGGCCA 1 cut(s) 1752
BarI GAAGNNNNNNTAC 2 cut(s) 739, 771
BauI CACGAG 1 cut(s) 1705
BbrPI CACGTG 1 cut(s) 1764
BbsI GAAGAC 4 cut(s) 270, 412, 791, 1270
BbvI GCAGC 5 cut(s) 378, 381, 925, 1242, 1345
BccI CCATC 7 cut(s) 50, 811, 894, 1063, 1226, 1742, 1923
BceAI ACGGC 4 cut(s) 401, 460, 909, 1838
BciT130I CCWGG 5 cut(s) 123, 1146, 1186, 1621, 1721
BciVI GTATCC 1 cut(s) 1236
BclI TGATCA 1 cut(s) 1122
BcoDI GTCTC 5 cut(s) 163, 974, 1021, 1181, 1899
BfaI CTAG 6 cut(s) 185, 284, 840, 990, 1283, 1731
BfoI RGCGCY 3 cut(s) 450, 504, 1915
BfuAI ACCTGC 1 cut(s) 734
BfuI GTATCC 1 cut(s) 1236
Bme1390I CCNGG 5 cut(s) 123, 1146, 1186, 1621, 1721
BmgBI CACGTC 1 cut(s) 535
BmgT120I GGNCC 2 cut(s) 752, 1851
BmiI GGNNCC 4 cut(s) 172, 434, 767, 1836
BmrFI CCNGG 5 cut(s) 123, 1146, 1186, 1621, 1721
BmrI ACTGGG 1 cut(s) 764
BmsI GCATC 4 cut(s) 556, 1267, 1345, 1501
BmtI GCTAGC 1 cut(s) 843
BmuI ACTGGG 1 cut(s) 764
BpiI GAAGAC 4 cut(s) 270, 412, 791, 1270
BplI GAGNNNNNCTC 4 cut(s) 132, 164, 1163, 1195
Bpu10I CCTNAGC 1 cut(s) 1654
BpuEI CTTGAG 3 cut(s) 83, 752, 1427
Bsa29I ATCGAT 1 cut(s) 1509
BsaAI YACGTR 1 cut(s) 1764
BsaBI GATNNNNATC 1 cut(s) 1887
BsaHI GRCGYC 2 cut(s) 94, 222
BsaI GGTCTC 1 cut(s) 1181
BsaJI CCNNGG 5 cut(s) 121, 925, 1184, 1534, 1619
BsaWI WCCGGW 2 cut(s) 167, 826
Bsc4I CCNNNNNNNGG 8 cut(s) 122, 140, 274, 775, 994, 1400, 1480, 1860
Bse1I ACTGG 3 cut(s) 770, 1753, 1930
Bse8I GATNNNNATC 1 cut(s) 1887
BseBI CCWGG 5 cut(s) 123, 1146, 1186, 1621, 1721
BseCI ATCGAT 1 cut(s) 1509
BseDI CCNNGG 5 cut(s) 121, 925, 1184, 1534, 1619
BseGI GGATG 4 cut(s) 42, 316, 492, 1078
BseJI GATNNNNATC 1 cut(s) 1887
BseLI CCNNNNNNNGG 8 cut(s) 122, 140, 274, 775, 994, 1400, 1480, 1860
BseMII CTCAG 4 cut(s) 498, 651, 1221, 1310
BseNI ACTGG 3 cut(s) 770, 1753, 1930
BseRI GAGGAG 5 cut(s) 26, 496, 707, 710, 713
BseSI GKGCMC 1 cut(s) 1620
BseXI GCAGC 5 cut(s) 378, 381, 925, 1242, 1345
Bsh1236I CGCG 2 cut(s) 25, 368
BshFI GGCC 6 cut(s) 754, 924, 1393, 1652, 1752, 1853
BshVI ATCGAT 1 cut(s) 1509
BsiSI CCGG 3 cut(s) 168, 260, 827
BslFI GGGAC 1 cut(s) 808
BslI CCNNNNNNNGG 8 cut(s) 122, 140, 274, 775, 994, 1400, 1480, 1860
BsmAI GTCTC 5 cut(s) 163, 974, 1021, 1181, 1899
BsmBI CGTCTC 1 cut(s) 163
BsmFI GGGAC 1 cut(s) 808
BsnI GGCC 6 cut(s) 754, 924, 1393, 1652, 1752, 1853
Bso31I GGTCTC 1 cut(s) 1181
Bsp1286I GDGCHC 1 cut(s) 1620
Bsp143I GATC 8 cut(s) 291, 386, 727, 1075, 1122, 1311, 1789, 1882
BspANI GGCC 6 cut(s) 754, 924, 1393, 1652, 1752, 1853
BspCNI CTCAG 4 cut(s) 499, 652, 1222, 1309
BspDI ATCGAT 1 cut(s) 1509
BspFNI CGCG 2 cut(s) 25, 368
BspLI GGNNCC 4 cut(s) 172, 434, 767, 1836
BspMI ACCTGC 1 cut(s) 734
BspOI GCTAGC 1 cut(s) 843
BspPI GGATC 3 cut(s) 394, 1070, 1877
BspQI GCTCTTC 2 cut(s) 133, 155
BspTNI GGTCTC 1 cut(s) 1181
BsrBI CCGCTC 1 cut(s) 17
BsrI ACTGG 3 cut(s) 770, 1753, 1930
BssECI CCNNGG 5 cut(s) 121, 925, 1184, 1534, 1619
BssMI GATC 8 cut(s) 291, 386, 727, 1075, 1122, 1311, 1789, 1882
BssNI GRCGYC 2 cut(s) 94, 222
BssSI CACGAG 1 cut(s) 1705
BssT1I CCWWGG 1 cut(s) 1534
Bst2BI CACGAG 1 cut(s) 1705
Bst2UI CCWGG 5 cut(s) 123, 1146, 1186, 1621, 1721
Bst4CI ACNGT 7 cut(s) 61, 68, 542, 707, 799, 1465, 1820
Bst6I CTCTTC 2 cut(s) 133, 155
BstACI GRCGYC 2 cut(s) 94, 222
BstBAI YACGTR 1 cut(s) 1764
BstC8I GCNNGC 5 cut(s) 146, 197, 357, 841, 1626
BstDEI CTNAG 5 cut(s) 507, 660, 1230, 1296, 1654
BstDSI CCRYGG 1 cut(s) 925
BstENI CCTNNNNNAGG 2 cut(s) 138, 272
BstF5I GGATG 4 cut(s) 42, 316, 492, 1078
BstFNI CGCG 2 cut(s) 25, 368
BstH2I RGCGCY 3 cut(s) 450, 504, 1915
BstHHI GCGC 5 cut(s) 25, 368, 449, 503, 1914
BstKTI GATC 8 cut(s) 294, 389, 730, 1078, 1125, 1314, 1792, 1885
BstMAI GTCTC 5 cut(s) 163, 974, 1021, 1181, 1899
BstMBI GATC 8 cut(s) 291, 386, 727, 1075, 1122, 1311, 1789, 1882
BstMWI GCNNNNNNNGC 5 cut(s) 93, 365, 432, 446, 500
BstNI CCWGG 5 cut(s) 123, 1146, 1186, 1621, 1721
BstNSI RCATGY 4 cut(s) 1002, 1628, 1692, 1700
BstSCI CCNGG 5 cut(s) 121, 1144, 1184, 1619, 1719
BstSLI GKGCMC 1 cut(s) 1620
BstUI CGCG 2 cut(s) 25, 368
BstV1I GCAGC 5 cut(s) 378, 381, 925, 1242, 1345
BstV2I GAAGAC 4 cut(s) 270, 412, 791, 1270
Bsu15I ATCGAT 1 cut(s) 1509
BsuI GTATCC 1 cut(s) 1236
BsuRI GGCC 6 cut(s) 754, 924, 1393, 1652, 1752, 1853
BsuTUI ATCGAT 1 cut(s) 1509
BtgI CCRYGG 1 cut(s) 925
BtrI CACGTC 1 cut(s) 535
BtsCI GGATG 4 cut(s) 42, 316, 492, 1078
BtsI GCAGTG 1 cut(s) 1067
BtsIMutI CAGTG 4 cut(s) 57, 777, 1067, 1760
BveI ACCTGC 1 cut(s) 734
Cac8I GCNNGC 5 cut(s) 146, 197, 357, 841, 1626
CfoI GCGC 5 cut(s) 25, 368, 449, 503, 1914
Cfr13I GGNCC 2 cut(s) 752, 1851
ClaI ATCGAT 1 cut(s) 1509
CseI GACGC 2 cut(s) 102, 211
CviAII CATG 8 cut(s) 976, 999, 1222, 1625, 1689, 1697, 1831, 1848
DdeI CTNAG 5 cut(s) 507, 660, 1230, 1296, 1654
DpnI GATC 8 cut(s) 293, 388, 729, 1077, 1124, 1313, 1791, 1884
DpnII GATC 8 cut(s) 291, 386, 727, 1075, 1122, 1311, 1789, 1882
EaeI YGGCCR 1 cut(s) 1750
Eam1104I CTCTTC 2 cut(s) 133, 155
EarI CTCTTC 2 cut(s) 133, 155
EciI GGCGGA 2 cut(s) 40, 626
Eco130I CCWWGG 1 cut(s) 1534
Eco147I AGGCCT 1 cut(s) 1652
Eco31I GGTCTC 1 cut(s) 1181
Eco32I GATATC 1 cut(s) 1507
Eco57I CTGAAG 2 cut(s) 361, 617
Eco72I CACGTG 1 cut(s) 1764
EcoNI CCTNNNNNAGG 2 cut(s) 138, 272
EcoO109I RGGNCCY 1 cut(s) 752
EcoRII CCWGG 5 cut(s) 121, 1144, 1184, 1619, 1719
EcoRV GATATC 1 cut(s) 1507
EcoT14I CCWWGG 1 cut(s) 1534
ErhI CCWWGG 1 cut(s) 1534
Esp3I CGTCTC 1 cut(s) 163
FaeI CATG 8 cut(s) 979, 1002, 1225, 1628, 1692, 1700, 1834, 1851
FalI AAGNNNNNCTT 2 cut(s) 137, 169
FaqI GGGAC 1 cut(s) 808
FatI CATG 8 cut(s) 975, 998, 1221, 1624, 1688, 1696, 1830, 1847
FauI CCCGC 2 cut(s) 89, 614
FbaI TGATCA 1 cut(s) 1122
FblI GTMKAC 2 cut(s) 63, 240
FokI GGATG 4 cut(s) 29, 323, 499, 1085
FspBI CTAG 6 cut(s) 185, 284, 840, 990, 1283, 1731
GlaI GCGC 5 cut(s) 24, 367, 448, 502, 1913
HaeII RGCGCY 3 cut(s) 450, 504, 1915
HaeIII GGCC 6 cut(s) 754, 924, 1393, 1652, 1752, 1853
HapII CCGG 3 cut(s) 168, 260, 827
HgaI GACGC 2 cut(s) 102, 211
HhaI GCGC 5 cut(s) 25, 368, 449, 503, 1914
Hin1I GRCGYC 2 cut(s) 94, 222
Hin1II CATG 8 cut(s) 979, 1002, 1225, 1628, 1692, 1700, 1834, 1851
Hin6I GCGC 5 cut(s) 23, 366, 447, 501, 1912
HinP1I GCGC 5 cut(s) 23, 366, 447, 501, 1912
HincII GTYRAC 2 cut(s) 241, 1602
HindII GTYRAC 2 cut(s) 241, 1602
HinfI GANTC 4 cut(s) 713, 832, 1419, 1427
HpaII CCGG 3 cut(s) 168, 260, 827
HphI GGTGA 6 cut(s) 542, 557, 598, 841, 1223, 1495
Hpy166II GTNNAC 7 cut(s) 64, 241, 538, 1214, 1602, 1702, 1761
Hpy8I GTNNAC 7 cut(s) 64, 241, 538, 1214, 1602, 1702, 1761
Hpy99I CGWCG 6 cut(s) 96, 195, 227, 242, 299, 784
HpyCH4III ACNGT 7 cut(s) 61, 68, 542, 707, 799, 1465, 1820
HpyCH4IV ACGT 4 cut(s) 452, 534, 1583, 1763
HpyCH4V TGCA 6 cut(s) 408, 743, 975, 1241, 1258, 1361
HpyF10VI GCNNNNNNNGC 5 cut(s) 93, 365, 432, 446, 500
HpyF3I CTNAG 5 cut(s) 507, 660, 1230, 1296, 1654
HpySE526I ACGT 4 cut(s) 452, 534, 1583, 1763
Hsp92I GRCGYC 2 cut(s) 94, 222
Hsp92II CATG 8 cut(s) 979, 1002, 1225, 1628, 1692, 1700, 1834, 1851
HspAI GCGC 5 cut(s) 23, 366, 447, 501, 1912
Ksp22I TGATCA 1 cut(s) 1122
Kzo9I GATC 8 cut(s) 291, 386, 727, 1075, 1122, 1311, 1789, 1882
LguI GCTCTTC 2 cut(s) 133, 155
LmnI GCTCC 6 cut(s) 22, 432, 1159, 1414, 1840, 1909
Lsp1109I GCAGC 5 cut(s) 378, 381, 925, 1242, 1345
LweI GCATC 4 cut(s) 556, 1267, 1345, 1501
MaeI CTAG 6 cut(s) 185, 284, 840, 990, 1283, 1731
MaeII ACGT 4 cut(s) 452, 534, 1583, 1763
MaeIII GTNAC 7 cut(s) 530, 820, 965, 1150, 1285, 1567, 1667
MalI GATC 8 cut(s) 293, 388, 729, 1077, 1124, 1313, 1791, 1884
MbiI CCGCTC 1 cut(s) 17
MboI GATC 8 cut(s) 291, 386, 727, 1075, 1122, 1311, 1789, 1882
MfeI CAATTG 1 cut(s) 403
MhlI GDGCHC 1 cut(s) 1620
MlsI TGGCCA 1 cut(s) 1752
MluCI AATT 6 cut(s) 212, 403, 410, 864, 891, 1093
MluNI TGGCCA 1 cut(s) 1752
MlyI GAGTC 1 cut(s) 722
MmeI TCCRAC 4 cut(s) 115, 1004, 1027, 1862
Mox20I TGGCCA 1 cut(s) 1752
MroXI GAANNNNTTC 1 cut(s) 1782
MscI TGGCCA 1 cut(s) 1752
MseI TTAA 6 cut(s) 215, 845, 1092, 1166, 1236, 1635
MslI CAYNNNNRTG 5 cut(s) 827, 882, 1118, 1629, 1846
Msp20I TGGCCA 1 cut(s) 1752
MspI CCGG 3 cut(s) 168, 260, 827
MspR9I CCNGG 5 cut(s) 123, 1146, 1186, 1621, 1721
MunI CAATTG 1 cut(s) 403
MvaI CCWGG 5 cut(s) 123, 1146, 1186, 1621, 1721
MvnI CGCG 2 cut(s) 25, 368
MwoI GCNNNNNNNGC 5 cut(s) 93, 365, 432, 446, 500
NdeII GATC 8 cut(s) 291, 386, 727, 1075, 1122, 1311, 1789, 1882
NheI GCTAGC 1 cut(s) 839
NlaIII CATG 8 cut(s) 979, 1002, 1225, 1628, 1692, 1700, 1834, 1851
NlaIV GGNNCC 4 cut(s) 172, 434, 767, 1836
NmeAIII GCCGAG 2 cut(s) 385, 461
NmuCI GTSAC 3 cut(s) 530, 820, 965
NspI RCATGY 4 cut(s) 1002, 1628, 1692, 1700
OliI CACNNNNGTG 1 cut(s) 827
PaeI GCATGC 1 cut(s) 1628
PceI AGGCCT 1 cut(s) 1652
PciSI GCTCTTC 2 cut(s) 133, 155
PcsI WCGNNNNNNNCGW 1 cut(s) 237
PdmI GAANNNNTTC 1 cut(s) 1782
PfeI GAWTC 3 cut(s) 832, 1419, 1427
PflFI GACNNNGTC 2 cut(s) 236, 907
PflMI CCANNNNNTGG 1 cut(s) 1400
PleI GAGTC 1 cut(s) 721
PmaCI CACGTG 1 cut(s) 1764
PmlI CACGTG 1 cut(s) 1764
PpsI GAGTC 1 cut(s) 721
Ppu21I YACGTR 1 cut(s) 1764
PshBI ATTAAT 1 cut(s) 1236
PsiI TTATAA 1 cut(s) 1521
Psp6I CCWGG 5 cut(s) 121, 1144, 1184, 1619, 1719
PspCI CACGTG 1 cut(s) 1764
PspGI CCWGG 5 cut(s) 121, 1144, 1184, 1619, 1719
PspN4I GGNNCC 4 cut(s) 172, 434, 767, 1836
PspPI GGNCC 2 cut(s) 752, 1851
PsrI GAACNNNNNNTAC 2 cut(s) 1573, 1605
PsyI GACNNNGTC 2 cut(s) 236, 907
RseI CAYNNNNRTG 5 cut(s) 827, 882, 1118, 1629, 1846
SalI GTCGAC 1 cut(s) 239
SapI GCTCTTC 2 cut(s) 133, 155
SaqAI TTAA 6 cut(s) 215, 845, 1092, 1166, 1236, 1635
Sau3AI GATC 8 cut(s) 291, 386, 727, 1075, 1122, 1311, 1789, 1882
Sau96I GGNCC 2 cut(s) 752, 1851
SchI GAGTC 1 cut(s) 722
ScrFI CCNGG 5 cut(s) 123, 1146, 1186, 1621, 1721
SduI GDGCHC 1 cut(s) 1620
SfaNI GCATC 4 cut(s) 556, 1267, 1345, 1501
SmiMI CAYNNNNRTG 5 cut(s) 827, 882, 1118, 1629, 1846
SmlI CTYRAG 3 cut(s) 98, 731, 1442
SmoI CTYRAG 3 cut(s) 98, 731, 1442
SphI GCATGC 1 cut(s) 1628
Sse9I AATT 6 cut(s) 212, 403, 410, 864, 891, 1093
SseBI AGGCCT 1 cut(s) 1652
SspMI CTAG 6 cut(s) 185, 284, 840, 990, 1283, 1731
StuI AGGCCT 1 cut(s) 1652
StyD4I CCNGG 5 cut(s) 121, 1144, 1184, 1619, 1719
StyI CCWWGG 1 cut(s) 1534
TaaI ACNGT 7 cut(s) 61, 68, 542, 707, 799, 1465, 1820
TaiI ACGT 4 cut(s) 455, 537, 1586, 1766
TaqI TCGA 9 cut(s) 201, 231, 240, 302, 835, 919, 1261, 1509, 1897
TasI AATT 6 cut(s) 212, 403, 410, 864, 891, 1093
TauI GCSGC 5 cut(s) 28, 87, 325, 611, 779
TfiI GAWTC 3 cut(s) 832, 1419, 1427
Tru1I TTAA 6 cut(s) 215, 845, 1092, 1166, 1236, 1635
Tru9I TTAA 6 cut(s) 215, 845, 1092, 1166, 1236, 1635
TscAI CASTG 4 cut(s) 64, 777, 1067, 1760
TseFI GTSAC 3 cut(s) 530, 820, 965
TseI GCWGC 5 cut(s) 391, 394, 913, 1255, 1358
Tsp45I GTSAC 3 cut(s) 530, 820, 965
TspDTI ATGAA 6 cut(s) 663, 1148, 1411, 1695, 1791, 1836
TspGWI ACGGA 3 cut(s) 1068, 1282, 1875
TspRI CASTG 4 cut(s) 64, 777, 1067, 1760
Tth111I GACNNNGTC 2 cut(s) 236, 907
Van91I CCANNNNNTGG 1 cut(s) 1400
VspI ATTAAT 1 cut(s) 1236
XagI CCTNNNNNAGG 2 cut(s) 138, 272
XapI RAATTY 2 cut(s) 410, 891
XceI RCATGY 4 cut(s) 1002, 1628, 1692, 1700
XcmI CCANNNNNNNNNTGG 1 cut(s) 1845
XmiI GTMKAC 2 cut(s) 63, 240
XmnI GAANNNNTTC 1 cut(s) 1782
XspI CTAG 6 cut(s) 185, 284, 840, 990, 1283, 1731
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.