Rh6AG414000

S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0013169
Physical Location & Seq
Reverse (-)
60953130 .. 60957618
4489 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG414000.1

Sequence Viewer

Length: 1380 bp
ATGTCCACATCCTCAGCCCGAGCACGGCTGGCTCTCATAGCCCTCCTCTCCGCCACCACGTTCTACTGTTTCTACAAATCTCGCCGCCTCAAGCAACTCCCCCTAAACCCTAACCCTAGCTCCTCTCCGCCTCGCAAAGGCAAGCTCTTCTTCATTTCCCAAACCGGAACCTCCAAAGCCCTAGCTCAGCGTCTTCTAAAACTTCTAGCCTCCAATGGCCTCGCCTTCGAGCTCGTCGACCCTAGCAGCTACGAGCCAGAGGACCTCCCCAAGGAGACCCTAGTCCTGATCGTCGCTTCCACCTGGGACGATGGACAACCGCCTTCTGGCGCCAAGTTCTTCACCAATTGGCTCGCGGAGAGCGCCGAGGACTTCAGAGTGGGGTCCATGCTGCTCTCGCAGTGCAGATTCTCGGTGTTCGGCGTCGGAAGCCGAGCCTACGGTGCGTCGTTCAATGCGGTGGGGAGGGACTTGTCGAAGTGGATGAGGGCCTTGGGCGCCGCCGAGATATTGCCGGTTTGGGAAGGTGACGTGGACGACGGGGATATAGACCAGGTCTTTGAGGCTTGGAGTGGTAAGCTGATGAGGTTTTTGAAGGCCGGTGTGGCCGAAAACGGTGTCGCTTTGGGTAATGGTGTTGATGCTGATGCTGAAGATGAAGCTGAGTATTCTGACGTGTCAGATTATGATGAGGAGGAGGAGGAGACTGTGGTTGAGTCTGGGGTTGTTGATCTTGAGGACATTGCAGGTAAAGCACCTTCAAGGGGGACGGTGACGGTGGCTAAGACTAAGACCAATGGGATAGTGAATGGTAAAAGAGATATGGTCACTCCGGTGATTCGTGCCAGCTTAGAGAAGCAGGGGTACAAAATTATTGGTTCGCATAGTGGTGTCAAAATTTGTAGATGGACAAAGTCACAGCTTAGAGGCCGAGGTGGTTGCTATAAACACTCGTTTTATGGCATAGAAAGTCATAGGTGCATGGAGGCAACCCCTAGTTTGGCATGTGCCAATAAATGTGTTTTCTGTTGGAGACATCATACAAACCCTGTTGGAAAGAGTTGGCAGTGGAAGATGGACGATCCATTAGAGATTGTGAAAACTGCCATAGACCTGCACAGTAAAATGATTAAGCAAATGAAAGGAGTTCCTGGAGTTACATTGGAGCGATTAACAGAGGGTCTCTCTCCCAGGCATTGTGCCTTGTCACTTGTTGGTGAACCTATTATGTATCCAGAGATTAATACACTGGTTGATGAGCTGCATCGTAGACAGATTTCTACTTTTCTAGTGACAAATGCTCAATTTCCTGAAAAAATTAGAATGCTGAAACCTATTACACAGGTTTGTATGTTTCATCACAATACGCTTGCACTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Flavodoxin_1 Radical_SAM Wyosine_form

Protein Analysis

459

Amino Acids

50.67

Weight (kDa)

7.12

Isoelectric Point (pI)

35.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Flavodoxin_1 PF00258 49 - 190 5.6e-27 Flavodoxin
Radical_SAM PF04055 334 - 447 7.4e-06 Radical SAM superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013169)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G75200
fragaria_vesca FvH4_2g39810 FvH4_2g39810
malus_domestica MD08G1031900.v1.1 MD15G1027800.v1.1
prunus_persica Prupe.1G379500_v2.0.a1 Prupe.1G379500_v2.0.a1
pyrus_communis pycom08g02520 pycom15g02380
rosa_chinensis RchiOBHm_Chr6g0301031
rosa_laevigata RLG00000011283
rosa_multiflora Rmu_co8483439.1_g000001
rosa_roxburghii Rroxscaffold_7G00167240
rosa_rugosa Rorug06G0300100
rosa_samantha Rh6AG414000 Rh6BG421000 Rh6CG428600 Rh6DG414000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 737, 1122
AccB1I GGYRCC 2 cut(s) 329, 497
AccI GTMKAC 2 cut(s) 237, 1270
AccII CGCG 1 cut(s) 356
AciI CCGC 7 cut(s) 51, 85, 128, 320, 356, 458, 501
AclWI GGATC 1 cut(s) 1076
AcoI YGGCCR 1 cut(s) 606
AcsI RAATTY 1 cut(s) 897
AcuI CTGAAG 2 cut(s) 358, 672
AcyI GRCGYC 3 cut(s) 330, 423, 498
AfaI GTAC 1 cut(s) 866
AfiI CCNNNNNNNGG 6 cut(s) 24, 137, 271, 326, 764, 1000
AflIII ACRYGT 1 cut(s) 675
AgsI TTSAA 3 cut(s) 454, 595, 762
AjiI CACGTC 2 cut(s) 532, 676
AjnI CCWGG 4 cut(s) 302, 552, 1150, 1190
AleI CACNNNNGTG 1 cut(s) 833
Alw21I GWGCWC 2 cut(s) 25, 234
Alw26I GTCTC 4 cut(s) 269, 698, 1027, 1187
AlwI GGATC 1 cut(s) 1076
Ama87I CYCGRG 1 cut(s) 18
AoxI GGCC 5 cut(s) 217, 489, 597, 606, 928
ApeKI GCWGC 3 cut(s) 246, 391, 1261
ApoI RAATTY 1 cut(s) 897
ArsI GACNNNNNNTTYG 4 cut(s) 542, 574, 603, 635
AseI ATTAAT 1 cut(s) 1242
AspLEI GCGC 3 cut(s) 332, 365, 500
AspS9I GGNCC 3 cut(s) 262, 384, 489
AsuHPI GGTGA 5 cut(s) 334, 539, 784, 847, 1229
AvaI CYCGRG 1 cut(s) 18
AvaII GGWCC 2 cut(s) 262, 384
BanI GGYRCC 2 cut(s) 329, 497
BanII GRGCYC 1 cut(s) 234
BarI GAAGNNNNNNTAC 4 cut(s) 742, 774, 848, 880
BbsI GAAGAC 1 cut(s) 185
Bbv12I GWGCWC 2 cut(s) 25, 234
BbvCI CCTCAGC 1 cut(s) 13
BbvI GCAGC 3 cut(s) 258, 378, 1248
BccI CCATC 3 cut(s) 305, 900, 1069
BceAI ACGGC 1 cut(s) 41
BcgI CGANNNNNNTGC 2 cut(s) 921, 955
BciT130I CCWGG 4 cut(s) 304, 554, 1152, 1192
BciVI GTATCC 1 cut(s) 1242
BcoDI GTCTC 4 cut(s) 269, 698, 1027, 1187
BfaI CTAG 7 cut(s) 117, 182, 206, 243, 281, 996, 1289
BfoI RGCGCY 3 cut(s) 333, 366, 501
BfuAI ACCTGC 2 cut(s) 737, 1122
BfuI GTATCC 1 cut(s) 1242
BglI GCCNNNNNGGC 1 cut(s) 605
BisI GCNGC 5 cut(s) 85, 247, 392, 501, 1262
BlpI GCTNAGC 1 cut(s) 186
BlsI GCNGC 5 cut(s) 86, 248, 393, 502, 1263
Bme1390I CCNGG 4 cut(s) 304, 554, 1152, 1192
Bme18I GGWCC 2 cut(s) 262, 384
BmeT110I CYCGRG 1 cut(s) 18
BmgBI CACGTC 2 cut(s) 532, 676
BmgT120I GGNCC 3 cut(s) 262, 384, 489
BmiI GGNNCC 4 cut(s) 169, 331, 385, 499
BmrFI CCNGG 4 cut(s) 304, 554, 1152, 1192
BmsI GCATC 3 cut(s) 631, 637, 1273
BoxI GACNNNNGTC 1 cut(s) 281
BpiI GAAGAC 1 cut(s) 185
BplI GAGNNNNNCTC 2 cut(s) 1169, 1201
BpmI CTGGAG 1 cut(s) 1173
Bpu10I CCTNAGC 1 cut(s) 13
Bpu1102I GCTNAGC 1 cut(s) 186
BpuEI CTTGAG 2 cut(s) 74, 755
BsaHI GRCGYC 3 cut(s) 330, 423, 498
BsaI GGTCTC 2 cut(s) 269, 1187
BsaJI CCNNGG 6 cut(s) 270, 303, 366, 492, 931, 1190
BsaWI WCCGGW 2 cut(s) 164, 832
BsaXI ACNNNNNCTCC 2 cut(s) 104, 134
Bsc4I CCNNNNNNNGG 6 cut(s) 24, 137, 271, 326, 764, 1000
Bse118I RCCGGY 2 cut(s) 514, 599
Bse1I ACTGG 1 cut(s) 1254
Bse3DI GCAATG 1 cut(s) 741
BseBI CCWGG 4 cut(s) 304, 554, 1152, 1192
BseDI CCNNGG 6 cut(s) 270, 303, 366, 492, 931, 1190
BseGI GGATG 2 cut(s) 8, 489
BseLI CCNNNNNNNGG 6 cut(s) 24, 137, 271, 326, 764, 1000
BseMI GCAATG 1 cut(s) 741
BseMII CTCAG 3 cut(s) 27, 200, 654
BseNI ACTGG 1 cut(s) 1254
BseRI GAGGAG 6 cut(s) 35, 112, 707, 710, 713, 716
BseXI GCAGC 3 cut(s) 258, 378, 1248
BsgI GTGCAG 2 cut(s) 424, 1100
Bsh1236I CGCG 1 cut(s) 356
BshFI GGCC 5 cut(s) 219, 491, 599, 608, 930
BshNI GGYRCC 2 cut(s) 329, 497
BsiHKAI GWGCWC 2 cut(s) 25, 234
BsiHKCI CYCGRG 1 cut(s) 18
BsiSI CCGG 4 cut(s) 165, 515, 600, 833
BslFI GGGAC 3 cut(s) 320, 482, 781
BslI CCNNNNNNNGG 6 cut(s) 24, 137, 271, 326, 764, 1000
BsmAI GTCTC 4 cut(s) 269, 698, 1027, 1187
BsmFI GGGAC 3 cut(s) 320, 482, 781
BsmI GAATGC 1 cut(s) 1329
BsnI GGCC 5 cut(s) 219, 491, 599, 608, 930
Bso31I GGTCTC 2 cut(s) 269, 1187
BsoBI CYCGRG 1 cut(s) 18
Bsp1286I GDGCHC 2 cut(s) 25, 234
Bsp143I GATC 3 cut(s) 288, 730, 1081
Bsp1720I GCTNAGC 1 cut(s) 186
BspACI CCGC 7 cut(s) 51, 85, 128, 320, 356, 458, 501
BspANI GGCC 5 cut(s) 219, 491, 599, 608, 930
BspCNI CTCAG 3 cut(s) 26, 199, 655
BspFNI CGCG 1 cut(s) 356
BspLI GGNNCC 4 cut(s) 169, 331, 385, 499
BspMI ACCTGC 2 cut(s) 737, 1122
BspPI GGATC 1 cut(s) 1076
BspQI GCTCTTC 1 cut(s) 152
BspT107I GGYRCC 2 cut(s) 329, 497
BspTNI GGTCTC 2 cut(s) 269, 1187
BsrDI GCAATG 1 cut(s) 741
BsrFI RCCGGY 2 cut(s) 514, 599
BsrI ACTGG 1 cut(s) 1254
BssAI RCCGGY 2 cut(s) 514, 599
BssECI CCNNGG 6 cut(s) 270, 303, 366, 492, 931, 1190
BssMI GATC 3 cut(s) 288, 730, 1081
BssNI GRCGYC 3 cut(s) 330, 423, 498
BssT1I CCWWGG 2 cut(s) 270, 492
Bst2UI CCWGG 4 cut(s) 304, 554, 1152, 1192
Bst4CI ACNGT 7 cut(s) 68, 443, 617, 709, 772, 778, 1121
Bst6I CTCTTC 1 cut(s) 152
BstACI GRCGYC 3 cut(s) 330, 423, 498
BstC8I GCNNGC 5 cut(s) 30, 143, 354, 847, 1371
BstDEI CTNAG 7 cut(s) 13, 186, 663, 783, 789, 850, 923
BstENI CCTNNNNNAGG 2 cut(s) 135, 269
BstF5I GGATG 2 cut(s) 8, 489
BstFNI CGCG 1 cut(s) 356
BstH2I RGCGCY 3 cut(s) 333, 366, 501
BstHHI GCGC 3 cut(s) 332, 365, 500
BstKTI GATC 3 cut(s) 291, 733, 1084
BstMAI GTCTC 4 cut(s) 269, 698, 1027, 1187
BstMBI GATC 3 cut(s) 288, 730, 1081
BstMWI GCNNNNNNNGC 9 cut(s) 29, 38, 362, 397, 429, 443, 497, 605, 752
BstNI CCWGG 4 cut(s) 304, 554, 1152, 1192
BstNSI RCATGY 1 cut(s) 1008
BstPAI GACNNNNGTC 1 cut(s) 281
BstSCI CCNGG 4 cut(s) 302, 552, 1150, 1190
BstUI CGCG 1 cut(s) 356
BstV1I GCAGC 3 cut(s) 258, 378, 1248
BstV2I GAAGAC 1 cut(s) 185
BsuI GTATCC 1 cut(s) 1242
BsuRI GGCC 5 cut(s) 219, 491, 599, 608, 930
BtrI CACGTC 2 cut(s) 532, 676
BtsCI GGATG 2 cut(s) 8, 489
BtsI GCAGTG 2 cut(s) 407, 1073
BtsIMutI CAGTG 3 cut(s) 407, 1073, 1247
BveI ACCTGC 2 cut(s) 737, 1122
Cac8I GCNNGC 5 cut(s) 30, 143, 354, 847, 1371
CfoI GCGC 3 cut(s) 332, 365, 500
Cfr10I RCCGGY 2 cut(s) 514, 599
Cfr13I GGNCC 3 cut(s) 262, 384, 489
CseI GACGC 3 cut(s) 179, 412, 435
CsiI ACCWGGT 1 cut(s) 552
Csp6I GTAC 1 cut(s) 865
CviAII CATG 3 cut(s) 388, 982, 1005
CviQI GTAC 1 cut(s) 865
DdeI CTNAG 7 cut(s) 13, 186, 663, 783, 789, 850, 923
DinI GGCGCC 2 cut(s) 331, 499
DpnI GATC 3 cut(s) 290, 732, 1083
DpnII GATC 3 cut(s) 288, 730, 1081
EaeI YGGCCR 1 cut(s) 606
Eam1104I CTCTTC 1 cut(s) 152
EarI CTCTTC 1 cut(s) 152
EciI GGCGGA 2 cut(s) 40, 117
Ecl136II GAGCTC 1 cut(s) 232
Eco130I CCWWGG 2 cut(s) 270, 492
Eco24I GRGCYC 1 cut(s) 234
Eco31I GGTCTC 2 cut(s) 269, 1187
Eco47I GGWCC 2 cut(s) 262, 384
Eco53kI GAGCTC 1 cut(s) 232
Eco57I CTGAAG 2 cut(s) 358, 672
Eco88I CYCGRG 1 cut(s) 18
EcoICRI GAGCTC 1 cut(s) 232
EcoNI CCTNNNNNAGG 2 cut(s) 135, 269
EcoO109I RGGNCCY 2 cut(s) 262, 489
EcoRII CCWGG 4 cut(s) 302, 552, 1150, 1190
EcoT14I CCWWGG 2 cut(s) 270, 492
EcoT38I GRGCYC 1 cut(s) 234
EgeI GGCGCC 2 cut(s) 331, 499
EheI GGCGCC 2 cut(s) 331, 499
ErhI CCWWGG 2 cut(s) 270, 492
FaeI CATG 3 cut(s) 391, 985, 1008
FalI AAGNNNNNCTT 2 cut(s) 134, 166
FaqI GGGAC 3 cut(s) 320, 482, 781
FatI CATG 3 cut(s) 387, 981, 1004
FblI GTMKAC 2 cut(s) 237, 1270
Fnu4HI GCNGC 5 cut(s) 85, 247, 392, 501, 1262
FokI GGATG 1 cut(s) 496
FriOI GRGCYC 1 cut(s) 234
Fsp4HI GCNGC 5 cut(s) 85, 247, 392, 501, 1262
FspBI CTAG 7 cut(s) 117, 182, 206, 243, 281, 996, 1289
GlaI GCGC 3 cut(s) 331, 364, 499
GluI GCNGC 5 cut(s) 85, 247, 392, 501, 1262
GsuI CTGGAG 1 cut(s) 1173
HaeII RGCGCY 3 cut(s) 333, 366, 501
HaeIII GGCC 5 cut(s) 219, 491, 599, 608, 930
HapII CCGG 4 cut(s) 165, 515, 600, 833
HgaI GACGC 3 cut(s) 179, 412, 435
HhaI GCGC 3 cut(s) 332, 365, 500
Hin1I GRCGYC 3 cut(s) 330, 423, 498
Hin1II CATG 3 cut(s) 391, 985, 1008
Hin6I GCGC 3 cut(s) 330, 363, 498
HinP1I GCGC 3 cut(s) 330, 363, 498
HincII GTYRAC 1 cut(s) 238
HindII GTYRAC 1 cut(s) 238
HinfI GANTC 3 cut(s) 408, 716, 838
HpaII CCGG 4 cut(s) 165, 515, 600, 833
HphI GGTGA 5 cut(s) 334, 539, 784, 847, 1229
Hpy166II GTNNAC 5 cut(s) 6, 238, 535, 1220, 1271
Hpy188I TCNGA 4 cut(s) 377, 428, 673, 682
Hpy188III TCNNGA 4 cut(s) 286, 734, 1235, 1310
Hpy8I GTNNAC 5 cut(s) 6, 238, 535, 1220, 1271
Hpy99I CGWCG 5 cut(s) 239, 296, 428, 451, 542
HpyAV CCTTC 5 cut(s) 235, 333, 518, 589, 768
HpyCH4III ACNGT 7 cut(s) 68, 443, 617, 709, 772, 778, 1121
HpyCH4IV ACGT 3 cut(s) 59, 531, 675
HpyCH4V TGCA 6 cut(s) 405, 746, 981, 1117, 1264, 1373
HpyF10VI GCNNNNNNNGC 9 cut(s) 29, 38, 362, 397, 429, 443, 497, 605, 752
HpyF3I CTNAG 7 cut(s) 13, 186, 663, 783, 789, 850, 923
HpySE526I ACGT 3 cut(s) 59, 531, 675
Hsp92I GRCGYC 3 cut(s) 330, 423, 498
Hsp92II CATG 3 cut(s) 391, 985, 1008
HspAI GCGC 3 cut(s) 330, 363, 498
KasI GGCGCC 2 cut(s) 329, 497
Kzo9I GATC 3 cut(s) 288, 730, 1081
LguI GCTCTTC 1 cut(s) 152
LmnI GCTCC 2 cut(s) 125, 1165
Lsp1109I GCAGC 3 cut(s) 258, 378, 1248
LweI GCATC 3 cut(s) 631, 637, 1273
MabI ACCWGGT 1 cut(s) 552
MaeI CTAG 7 cut(s) 117, 182, 206, 243, 281, 996, 1289
MaeII ACGT 3 cut(s) 59, 531, 675
MaeIII GTNAC 7 cut(s) 527, 772, 826, 915, 1156, 1206, 1291
MalI GATC 3 cut(s) 290, 732, 1083
MboI GATC 3 cut(s) 288, 730, 1081
MboII GAAGA 6 cut(s) 139, 142, 185, 331, 665, 1084
MfeI CAATTG 1 cut(s) 346
MhlI GDGCHC 2 cut(s) 25, 234
MluCI AATT 5 cut(s) 346, 870, 897, 1304, 1317
Mly113I GGCGCC 2 cut(s) 330, 498
MlyI GAGTC 1 cut(s) 725
MmeI TCCRAC 3 cut(s) 406, 1010, 1033
MseI TTAA 4 cut(s) 1131, 1172, 1242, 1378
MslI CAYNNNNRTG 2 cut(s) 833, 888
MspI CCGG 4 cut(s) 165, 515, 600, 833
MspR9I CCNGG 4 cut(s) 304, 554, 1152, 1192
MunI CAATTG 1 cut(s) 346
Mva1269I GAATGC 1 cut(s) 1329
MvaI CCWGG 4 cut(s) 304, 554, 1152, 1192
MvnI CGCG 1 cut(s) 356
MwoI GCNNNNNNNGC 9 cut(s) 29, 38, 362, 397, 429, 443, 497, 605, 752
NarI GGCGCC 2 cut(s) 330, 498
NdeII GATC 3 cut(s) 288, 730, 1081
NlaIII CATG 3 cut(s) 391, 985, 1008
NlaIV GGNNCC 4 cut(s) 169, 331, 385, 499
NmeAIII GCCGAG 4 cut(s) 391, 458, 529, 956
NmuCI GTSAC 6 cut(s) 527, 772, 826, 915, 1206, 1291
NspI RCATGY 1 cut(s) 1008
OliI CACNNNNGTG 1 cut(s) 833
PciSI GCTCTTC 1 cut(s) 152
PcsI WCGNNNNNNNCGW 1 cut(s) 234
PctI GAATGC 1 cut(s) 1329
PfeI GAWTC 2 cut(s) 408, 838
PflFI GACNNNGTC 2 cut(s) 554, 913
PfoI TCCNGGA 1 cut(s) 1150
PkrI GCNGC 5 cut(s) 86, 248, 393, 502, 1263
PleI GAGTC 1 cut(s) 724
PluTI GGCGCC 2 cut(s) 333, 501
PpsI GAGTC 1 cut(s) 724
PpuMI RGGWCCY 1 cut(s) 262
PshAI GACNNNNGTC 1 cut(s) 281
PshBI ATTAAT 1 cut(s) 1242
Psp124BI GAGCTC 1 cut(s) 234
Psp5II RGGWCCY 1 cut(s) 262
Psp6I CCWGG 4 cut(s) 302, 552, 1150, 1190
PspGI CCWGG 4 cut(s) 302, 552, 1150, 1190
PspN4I GGNNCC 4 cut(s) 169, 331, 385, 499
PspPI GGNCC 3 cut(s) 262, 384, 489
PspPPI RGGWCCY 1 cut(s) 262
PsyI GACNNNGTC 2 cut(s) 554, 913
RsaI GTAC 1 cut(s) 866
RsaNI GTAC 1 cut(s) 865
RseI CAYNNNNRTG 2 cut(s) 833, 888
SacI GAGCTC 1 cut(s) 234
SalI GTCGAC 1 cut(s) 236
SapI GCTCTTC 1 cut(s) 152
SaqAI TTAA 4 cut(s) 1131, 1172, 1242, 1378
SatI GCNGC 5 cut(s) 85, 247, 392, 501, 1262
Sau3AI GATC 3 cut(s) 288, 730, 1081
Sau96I GGNCC 3 cut(s) 262, 384, 489
SchI GAGTC 1 cut(s) 725
ScrFI CCNGG 4 cut(s) 304, 554, 1152, 1192
SduI GDGCHC 2 cut(s) 25, 234
SexAI ACCWGGT 1 cut(s) 552
SfaNI GCATC 3 cut(s) 631, 637, 1273
SfiI GGCCNNNNNGGCC 1 cut(s) 605
SfoI GGCGCC 2 cut(s) 331, 499
SinI GGWCC 2 cut(s) 262, 384
SmiMI CAYNNNNRTG 2 cut(s) 833, 888
SmlI CTYRAG 2 cut(s) 89, 734
SmoI CTYRAG 2 cut(s) 89, 734
Sse9I AATT 5 cut(s) 346, 870, 897, 1304, 1317
SsiI CCGC 7 cut(s) 51, 85, 128, 320, 356, 458, 501
SspDI GGCGCC 2 cut(s) 329, 497
SspMI CTAG 7 cut(s) 117, 182, 206, 243, 281, 996, 1289
SstI GAGCTC 1 cut(s) 234
StyD4I CCNGG 4 cut(s) 302, 552, 1150, 1190
StyI CCWWGG 2 cut(s) 270, 492
TaaI ACNGT 7 cut(s) 68, 443, 617, 709, 772, 778, 1121
TaiI ACGT 3 cut(s) 62, 534, 678
TaqI TCGA 3 cut(s) 228, 237, 476
TasI AATT 5 cut(s) 346, 870, 897, 1304, 1317
TauI GCSGC 2 cut(s) 87, 503
TfiI GAWTC 2 cut(s) 408, 838
Tru1I TTAA 4 cut(s) 1131, 1172, 1242, 1378
Tru9I TTAA 4 cut(s) 1131, 1172, 1242, 1378
TscAI CASTG 3 cut(s) 407, 1073, 1254
TseFI GTSAC 6 cut(s) 527, 772, 826, 915, 1206, 1291
TseI GCWGC 3 cut(s) 246, 391, 1261
Tsp45I GTSAC 6 cut(s) 527, 772, 826, 915, 1206, 1291
TspDTI ATGAA 4 cut(s) 142, 672, 1154, 1346
TspRI CASTG 3 cut(s) 407, 1073, 1254
Tth111I GACNNNGTC 2 cut(s) 554, 913
VpaK11BI GGWCC 2 cut(s) 262, 384
VspI ATTAAT 1 cut(s) 1242
XagI CCTNNNNNAGG 2 cut(s) 135, 269
XapI RAATTY 1 cut(s) 897
XceI RCATGY 1 cut(s) 1008
XmiI GTMKAC 2 cut(s) 237, 1270
XspI CTAG 7 cut(s) 117, 182, 206, 243, 281, 996, 1289
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.