Rorug06G0300100

S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0013169
Physical Location & Seq
Reverse (-)
45209466 .. 45211551
2086 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0300100.1

Sequence Viewer

Length: 1509 bp
ATGAAAGCGGAATTTGAAATGGTTCATGGGACTGTGAATATGATCCAACCCCATGAAACTAATTGGATTAACAATTCGACCTCCGTTAATCCATTCCCAAAACCTGTAAGCGATCTCTCTCAGTGGGTAGAGCACATAACGAAGCAACTCATCGATGACTTGCCGGAAACAGCCACCACCACGGCAGACACTTTGCACACTGCAGCAACATCCGGGGATCAGTACTTCATCCCCTCGTCACATTCCGGAGAGTTTAGTCCGAGAAAAGCCCCCAGGAAAAACTACGGCGACGACACAAGTGCTGATCGTGCAGATTACGACTTGCAGTGGGGAAATGAGCTGCAACTCCATGCAGAAAACAACAGGAGAGCTAGAGGGTTGAGTAAGCTTGACCAGCATGGCTTAACACTAATTACTCTTCTGTTCGAATGTGCGGTTGCAATCTCCGTGGAGAACCCCGGCGAGGCACATCAGATGCTACTTGAGCTCACCCAGATGGCCTCGCCGTATGGGCCATCCTGCGCGGAGCGCGTGGTGGCATATTTTGCCAAGGCCATGTCGAGTAGGGTTTTGAACTCGTGGCTAGGCATTTGCTCTCCTTTGGTCAACTACAAAAGCATTCATGGTGCTTTTCAAGTCTTTAACACTATCTCGCCTTTCATCAAGTTCGCTCACTTCACTTCCAACCAGGCCATCCTCGAAGCCTTTCACCGCCGTGACAGAGTGCACATTATCGACCTCGATATCATGCAGGGTTTGCAATGGCCAGCTCTTTTTCACATCCTCGCCACACGCATGGAAGGGCCTCCACACGTACGAATGACCGGTTTCGGCGCCTCATCTGAGGTATTGCTTGAGACCGGAAAGCAACTTTCAAATTTTGCCAGGCGACTGGGCTTGTCATTTGAGTTTCATCCAATCACGAGGAAATTCGGTGACCACCTAGACACTTCAATGGTGCAAGTGCGGAGGGGAGAGACTGTAGCAGTGCATTGGCTACAGCACTCACTCTACGATGCAACAGGACCCGACTGGAGAACAATGAAACTCATCGAAGAACTATCCCCGAGAATCGTCACATTGGTGGAGCAAGACATGTCGCACAATGGGGGCTCGTTTTTGGACCGTTTTGTCGGGTCTTTGCACTACTATTCAACTATGTTTGATTCTCTTGAAGCCTATTTGCCCAGTGATAACCCTAACAGGCATTTAGTTGAACACAGCTTGATGTATAGGGAAATCAACAACATATTGGCAGTAGGCGGGCCAGCAAGGAGCGGAGAAGACAAGTTTAAGCATTGGAGGAGTGAGCTCGGGGCTAGCAATAGCTTCATGCAAGTAGGTATGAGTGGAAATTCGATGGCTCAAGCGCAGTTGATATTGAACATGTTTCCTCCTAGTAATGGTTATAACCTTGTGCAAGGAGATGGGACGATTAGGCTTGGGTGGAAGGAAACTAGTTTGTATGTTGCCTCAGCATGGACTTGTTCTCATGCTTCATCAAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Flavodoxin_1 Radical_SAM Wyosine_form

Protein Analysis

502

Amino Acids

56.34

Weight (kDa)

6.17

Isoelectric Point (pI)

39.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GRAS PF03514 138 - 495 1.4e-111 GRAS domain family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013169)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G75200
fragaria_vesca FvH4_2g39810 FvH4_2g39810
malus_domestica MD08G1031900.v1.1 MD15G1027800.v1.1
prunus_persica Prupe.1G379500_v2.0.a1 Prupe.1G379500_v2.0.a1
pyrus_communis pycom08g02520 pycom15g02380
rosa_chinensis RchiOBHm_Chr6g0301031
rosa_laevigata RLG00000011283
rosa_multiflora Rmu_co8483439.1_g000001
rosa_roxburghii Rroxscaffold_7G00167240
rosa_rugosa Rorug06G0300100
rosa_samantha Rh6AG414000 Rh6BG421000 Rh6CG428600 Rh6DG414000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1410
AasI GACNNNNNNGTC 1 cut(s) 1130
AccB1I GGYRCC 1 cut(s) 833
AccBSI CCGCTC 1 cut(s) 1278
AccII CGCG 2 cut(s) 524, 531
AccIII TCCGGA 1 cut(s) 245
AciI CCGC 7 cut(s) 8, 434, 524, 712, 967, 1263, 1278
AclWI GGATC 2 cut(s) 37, 225
AcoI YGGCCR 1 cut(s) 764
AcsI RAATTY 4 cut(s) 11, 877, 929, 1354
AcyI GRCGYC 1 cut(s) 834
AfaI GTAC 2 cut(s) 224, 816
AfiI CCNNNNNNNGG 3 cut(s) 463, 1403, 1479
AflIII ACRYGT 3 cut(s) 811, 1095, 1386
AgeI ACCGGT 1 cut(s) 824
AgsI TTSAA 9 cut(s) 17, 574, 635, 876, 954, 1155, 1175, 1217, 1384
AhlI ACTAGT 1 cut(s) 1457
AjnI CCWGG 3 cut(s) 272, 687, 884
AleI CACNNNNGTG 2 cut(s) 714, 1082
AluBI AGCT 8 cut(s) 340, 371, 388, 487, 770, 1224, 1312, 1329
AluI AGCT 8 cut(s) 340, 371, 388, 487, 770, 1224, 1312, 1329
Alw21I GWGCWC 4 cut(s) 135, 489, 729, 1314
Alw26I GTCTC 2 cut(s) 851, 971
Alw44I GTGCAC 1 cut(s) 725
AlwI GGATC 2 cut(s) 37, 225
Ama87I CYCGRG 2 cut(s) 1066, 1313
Aor13HI TCCGGA 1 cut(s) 245
AoxI GGCC 7 cut(s) 498, 512, 552, 690, 764, 803, 1265
ApaLI GTGCAC 1 cut(s) 725
ApeKI GCWGC 2 cut(s) 203, 340
ApoI RAATTY 4 cut(s) 11, 877, 929, 1354
AsiGI ACCGGT 1 cut(s) 824
Asp700I GAANNNNTTC 2 cut(s) 21, 705
AspLEI GCGC 4 cut(s) 524, 531, 836, 1372
AspS9I GGNCC 5 cut(s) 512, 803, 1025, 1123, 1265
AsuC2I CCSGG 2 cut(s) 214, 459
AsuHPI GGTGA 3 cut(s) 481, 701, 947
AsuII TTCGAA 1 cut(s) 426
AsuNHI GCTAGC 1 cut(s) 1319
AvaI CYCGRG 2 cut(s) 1066, 1313
AvaII GGWCC 2 cut(s) 1025, 1123
BaeGI GKGCMC 1 cut(s) 729
BalI TGGCCA 1 cut(s) 766
BanI GGYRCC 1 cut(s) 833
BanII GRGCYC 3 cut(s) 489, 1115, 1314
BauI CACGAG 2 cut(s) 577, 922
BbsI GAAGAC 1 cut(s) 1290
Bbv12I GWGCWC 4 cut(s) 135, 489, 729, 1314
BbvCI CCTCAGC 1 cut(s) 1474
BbvI GCAGC 2 cut(s) 215, 327
BccI CCATC 5 cut(s) 490, 523, 701, 1354, 1421
BceAI ACGGC 4 cut(s) 198, 301, 490, 699
BcgI CGANNNNNNTGC 2 cut(s) 281, 315
BciT130I CCWGG 3 cut(s) 274, 689, 886
BcnI CCSGG 2 cut(s) 214, 459
BcoDI GTCTC 2 cut(s) 851, 971
BcuI ACTAGT 1 cut(s) 1457
BfaI CTAG 6 cut(s) 372, 584, 944, 1320, 1398, 1458
BfmI CTRYAG 3 cut(s) 201, 981, 998
BfoI RGCGCY 1 cut(s) 837
BglI GCCNNNNNGGC 1 cut(s) 511
BisI GCNGC 2 cut(s) 204, 341
BlsI GCNGC 2 cut(s) 205, 342
BmcAI AGTACT 1 cut(s) 224
Bme1390I CCNGG 5 cut(s) 214, 274, 459, 689, 886
Bme18I GGWCC 2 cut(s) 1025, 1123
BmeT110I CYCGRG 2 cut(s) 1066, 1313
BmgT120I GGNCC 5 cut(s) 512, 803, 1025, 1123, 1265
BmiI GGNNCC 2 cut(s) 835, 1027
BmrFI CCNGG 5 cut(s) 214, 274, 459, 689, 886
BmrI ACTGGG 2 cut(s) 902, 1182
BmsI GCATC 2 cut(s) 465, 1006
BmtI GCTAGC 1 cut(s) 1323
BmuI ACTGGG 2 cut(s) 902, 1182
BpiI GAAGAC 1 cut(s) 1290
BpmI CTGGAG 1 cut(s) 1054
Bpu10I CCTNAGC 1 cut(s) 1474
Bpu14I TTCGAA 1 cut(s) 426
BpuEI CTTGAG 3 cut(s) 503, 875, 1350
BpuMI CCSGG 2 cut(s) 214, 459
Bsa29I ATCGAT 1 cut(s) 153
BsaAI YACGTR 1 cut(s) 814
BsaHI GRCGYC 1 cut(s) 834
BsaI GGTCTC 1 cut(s) 851
BsaJI CCNNGG 6 cut(s) 180, 213, 272, 447, 457, 549
BsaWI WCCGGW 3 cut(s) 245, 824, 860
BsaXI ACNNNNNCTCC 2 cut(s) 966, 996
Bsc4I CCNNNNNNNGG 3 cut(s) 463, 1403, 1479
Bse118I RCCGGY 1 cut(s) 824
Bse1I ACTGG 3 cut(s) 897, 1037, 1188
Bse3DI GCAATG 1 cut(s) 767
BseAI TCCGGA 1 cut(s) 245
BseBI CCWGG 3 cut(s) 274, 689, 886
BseCI ATCGAT 1 cut(s) 153
BseDI CCNNGG 6 cut(s) 180, 213, 272, 447, 457, 549
BseGI GGATG 6 cut(s) 209, 228, 515, 693, 780, 913
BseLI CCNNNNNNNGG 3 cut(s) 463, 1403, 1479
BseMI GCAATG 1 cut(s) 767
BseMII CTCAG 3 cut(s) 134, 834, 1488
BseNI ACTGG 3 cut(s) 897, 1037, 1188
BseRI GAGGAG 1 cut(s) 1318
BseSI GKGCMC 1 cut(s) 729
BseXI GCAGC 2 cut(s) 215, 327
BsgI GTGCAG 1 cut(s) 330
Bsh1236I CGCG 2 cut(s) 524, 531
BshFI GGCC 7 cut(s) 500, 514, 554, 692, 766, 805, 1267
BshNI GGYRCC 1 cut(s) 833
BshTI ACCGGT 1 cut(s) 824
BshVI ATCGAT 1 cut(s) 153
BsiHKAI GWGCWC 4 cut(s) 135, 489, 729, 1314
BsiHKCI CYCGRG 2 cut(s) 1066, 1313
BsiSI CCGG 6 cut(s) 164, 213, 246, 459, 825, 861
BsiWI CGTACG 1 cut(s) 814
BslFI GGGAC 2 cut(s) 43, 1444
BslI CCNNNNNNNGG 3 cut(s) 463, 1403, 1479
BsmAI GTCTC 2 cut(s) 851, 971
BsmFI GGGAC 2 cut(s) 43, 1444
BsmI GAATGC 1 cut(s) 618
BsnI GGCC 7 cut(s) 500, 514, 554, 692, 766, 805, 1267
Bso31I GGTCTC 1 cut(s) 851
BsoBI CYCGRG 2 cut(s) 1066, 1313
Bsp119I TTCGAA 1 cut(s) 426
Bsp1286I GDGCHC 5 cut(s) 135, 489, 729, 1115, 1314
Bsp13I TCCGGA 1 cut(s) 245
Bsp143I GATC 4 cut(s) 42, 112, 217, 304
BspACI CCGC 7 cut(s) 8, 434, 524, 712, 967, 1263, 1278
BspANI GGCC 7 cut(s) 500, 514, 554, 692, 766, 805, 1267
BspCNI CTCAG 3 cut(s) 133, 835, 1487
BspDI ATCGAT 1 cut(s) 153
BspEI TCCGGA 1 cut(s) 245
BspFNI CGCG 2 cut(s) 524, 531
BspLI GGNNCC 2 cut(s) 835, 1027
BspMAI CTGCAG 1 cut(s) 205
BspOI GCTAGC 1 cut(s) 1323
BspPI GGATC 2 cut(s) 37, 225
BspT104I TTCGAA 1 cut(s) 426
BspT107I GGYRCC 1 cut(s) 833
BspTNI GGTCTC 1 cut(s) 851
BsrBI CCGCTC 1 cut(s) 1278
BsrDI GCAATG 1 cut(s) 767
BsrFI RCCGGY 1 cut(s) 824
BsrI ACTGG 3 cut(s) 897, 1037, 1188
BssAI RCCGGY 1 cut(s) 824
BssECI CCNNGG 6 cut(s) 180, 213, 272, 447, 457, 549
BssMI GATC 4 cut(s) 42, 112, 217, 304
BssNI GRCGYC 1 cut(s) 834
BssSI CACGAG 2 cut(s) 577, 922
BssT1I CCWWGG 1 cut(s) 549
Bst2BI CACGAG 2 cut(s) 577, 922
Bst2UI CCWGG 3 cut(s) 274, 689, 886
Bst4CI ACNGT 3 cut(s) 34, 982, 1127
Bst6I CTCTTC 1 cut(s) 423
BstACI GRCGYC 1 cut(s) 834
BstAPI GCANNNNNTGC 2 cut(s) 545, 757
BstBAI YACGTR 1 cut(s) 814
BstBI TTCGAA 1 cut(s) 426
BstC8I GCNNGC 4 cut(s) 768, 1265, 1269, 1321
BstDEI CTNAG 3 cut(s) 120, 843, 1474
BstDSI CCRYGG 2 cut(s) 180, 447
BstEII GGTNACC 1 cut(s) 935
BstF5I GGATG 6 cut(s) 209, 228, 515, 693, 780, 913
BstFNI CGCG 2 cut(s) 524, 531
BstH2I RGCGCY 1 cut(s) 837
BstHHI GCGC 4 cut(s) 524, 531, 836, 1372
BstKTI GATC 4 cut(s) 45, 115, 220, 307
BstMAI GTCTC 2 cut(s) 851, 971
BstMBI GATC 4 cut(s) 42, 112, 217, 304
BstMWI GCNNNNNNNGC 7 cut(s) 308, 394, 484, 511, 528, 545, 757
BstNI CCWGG 3 cut(s) 274, 689, 886
BstNSI RCATGY 2 cut(s) 1099, 1390
BstPI GGTNACC 1 cut(s) 935
BstSCI CCNGG 5 cut(s) 212, 272, 457, 687, 884
BstSFI CTRYAG 3 cut(s) 201, 981, 998
BstSLI GKGCMC 1 cut(s) 729
BstUI CGCG 2 cut(s) 524, 531
BstV1I GCAGC 2 cut(s) 215, 327
BstV2I GAAGAC 1 cut(s) 1290
BstXI CCANNNNNNTGG 2 cut(s) 796, 892
Bsu15I ATCGAT 1 cut(s) 153
BsuRI GGCC 7 cut(s) 500, 514, 554, 692, 766, 805, 1267
BsuTUI ATCGAT 1 cut(s) 153
BtgI CCRYGG 2 cut(s) 180, 447
BtsCI GGATG 6 cut(s) 209, 228, 515, 693, 780, 913
BtsI GCAGTG 3 cut(s) 198, 332, 993
BtsIMutI CAGTG 5 cut(s) 128, 198, 332, 993, 1195
Cac8I GCNNGC 4 cut(s) 768, 1265, 1269, 1321
CfoI GCGC 4 cut(s) 524, 531, 836, 1372
Cfr10I RCCGGY 1 cut(s) 824
Cfr13I GGNCC 5 cut(s) 512, 803, 1025, 1123, 1265
ClaI ATCGAT 1 cut(s) 153
Csp6I GTAC 2 cut(s) 223, 815
CspAI ACCGGT 1 cut(s) 824
CspCI CAANNNNNGTGG 2 cut(s) 429, 464
CviQI GTAC 2 cut(s) 223, 815
DdeI CTNAG 3 cut(s) 120, 843, 1474
DinI GGCGCC 1 cut(s) 835
DpnI GATC 4 cut(s) 44, 114, 219, 306
DpnII GATC 4 cut(s) 42, 112, 217, 304
DrdI GACNNNNNNGTC 1 cut(s) 1130
DseDI GACNNNNNNGTC 1 cut(s) 1130
EaeI YGGCCR 1 cut(s) 764
Eam1104I CTCTTC 1 cut(s) 423
EarI CTCTTC 1 cut(s) 423
Ecl136II GAGCTC 2 cut(s) 487, 1312
Eco130I CCWWGG 1 cut(s) 549
Eco24I GRGCYC 3 cut(s) 489, 1115, 1314
Eco31I GGTCTC 1 cut(s) 851
Eco32I GATATC 1 cut(s) 745
Eco47I GGWCC 2 cut(s) 1025, 1123
Eco53kI GAGCTC 2 cut(s) 487, 1312
Eco88I CYCGRG 2 cut(s) 1066, 1313
Eco91I GGTNACC 1 cut(s) 935
EcoICRI GAGCTC 2 cut(s) 487, 1312
EcoO109I RGGNCCY 2 cut(s) 803, 1025
EcoO65I GGTNACC 1 cut(s) 935
EcoRII CCWGG 3 cut(s) 272, 687, 884
EcoRV GATATC 1 cut(s) 745
EcoT14I CCWWGG 1 cut(s) 549
EcoT38I GRGCYC 3 cut(s) 489, 1115, 1314
EgeI GGCGCC 1 cut(s) 835
EheI GGCGCC 1 cut(s) 835
ErhI CCWWGG 1 cut(s) 549
FaqI GGGAC 2 cut(s) 43, 1444
FauI CCCGC 1 cut(s) 1256
Fnu4HI GCNGC 2 cut(s) 204, 341
FokI GGATG 6 cut(s) 196, 215, 502, 680, 767, 900
FriOI GRGCYC 3 cut(s) 489, 1115, 1314
Fsp4HI GCNGC 2 cut(s) 204, 341
FspBI CTAG 6 cut(s) 372, 584, 944, 1320, 1398, 1458
GlaI GCGC 4 cut(s) 523, 530, 835, 1371
GluI GCNGC 2 cut(s) 204, 341
GsuI CTGGAG 1 cut(s) 1054
HaeII RGCGCY 1 cut(s) 837
HaeIII GGCC 7 cut(s) 500, 514, 554, 692, 766, 805, 1267
HapII CCGG 6 cut(s) 164, 213, 246, 459, 825, 861
HhaI GCGC 4 cut(s) 524, 531, 836, 1372
Hin1I GRCGYC 1 cut(s) 834
Hin6I GCGC 4 cut(s) 522, 529, 834, 1370
HinP1I GCGC 4 cut(s) 522, 529, 834, 1370
HincII GTYRAC 1 cut(s) 607
HindII GTYRAC 1 cut(s) 607
HindIII AAGCTT 1 cut(s) 386
HinfI GANTC 2 cut(s) 1071, 1166
HpaII CCGG 6 cut(s) 164, 213, 246, 459, 825, 861
HphI GGTGA 3 cut(s) 481, 701, 947
Hpy166II GTNNAC 2 cut(s) 607, 727
Hpy188I TCNGA 3 cut(s) 261, 474, 844
Hpy188III TCNNGA 4 cut(s) 246, 922, 1172, 1503
Hpy8I GTNNAC 2 cut(s) 607, 727
Hpy99I CGWCG 1 cut(s) 293
HpyAV CCTTC 2 cut(s) 794, 1444
HpyCH4III ACNGT 3 cut(s) 34, 982, 1127
HpyCH4IV ACGT 1 cut(s) 813
HpyF10VI GCNNNNNNNGC 7 cut(s) 308, 394, 484, 511, 528, 545, 757
HpyF3I CTNAG 3 cut(s) 120, 843, 1474
HpySE526I ACGT 1 cut(s) 813
Hsp92I GRCGYC 1 cut(s) 834
HspAI GCGC 4 cut(s) 522, 529, 834, 1370
KasI GGCGCC 1 cut(s) 833
Kpn2I TCCGGA 1 cut(s) 245
Kzo9I GATC 4 cut(s) 42, 112, 217, 304
LmnI GCTCC 3 cut(s) 526, 1087, 1275
Lsp1109I GCAGC 2 cut(s) 215, 327
LweI GCATC 2 cut(s) 465, 1006
MaeI CTAG 6 cut(s) 372, 584, 944, 1320, 1398, 1458
MaeII ACGT 1 cut(s) 813
MaeIII GTNAC 4 cut(s) 237, 716, 935, 1075
MalI GATC 4 cut(s) 44, 114, 219, 306
MbiI CCGCTC 1 cut(s) 1278
MboI GATC 4 cut(s) 42, 112, 217, 304
MboII GAAGA 3 cut(s) 410, 1067, 1295
MhlI GDGCHC 5 cut(s) 135, 489, 729, 1115, 1314
MlsI TGGCCA 1 cut(s) 766
MluCI AATT 7 cut(s) 11, 61, 73, 411, 877, 929, 1354
MluNI TGGCCA 1 cut(s) 766
Mly113I GGCGCC 1 cut(s) 834
MmeI TCCRAC 2 cut(s) 70, 708
Mox20I TGGCCA 1 cut(s) 766
MroI TCCGGA 1 cut(s) 245
MroXI GAANNNNTTC 2 cut(s) 21, 705
MscI TGGCCA 1 cut(s) 766
MseI TTAA 5 cut(s) 69, 87, 404, 642, 1293
MslI CAYNNNNRTG 5 cut(s) 494, 714, 794, 953, 1082
Msp20I TGGCCA 1 cut(s) 766
MspI CCGG 6 cut(s) 164, 213, 246, 459, 825, 861
MspR9I CCNGG 5 cut(s) 214, 274, 459, 689, 886
Mva1269I GAATGC 1 cut(s) 618
MvaI CCWGG 3 cut(s) 274, 689, 886
MvnI CGCG 2 cut(s) 524, 531
MwoI GCNNNNNNNGC 7 cut(s) 308, 394, 484, 511, 528, 545, 757
NarI GGCGCC 1 cut(s) 834
NciI CCSGG 2 cut(s) 214, 459
NdeII GATC 4 cut(s) 42, 112, 217, 304
NheI GCTAGC 1 cut(s) 1319
NlaIV GGNNCC 2 cut(s) 835, 1027
NmuCI GTSAC 4 cut(s) 237, 716, 935, 1075
NspI RCATGY 2 cut(s) 1099, 1390
NspV TTCGAA 1 cut(s) 426
OliI CACNNNNGTG 2 cut(s) 714, 1082
PciI ACATGT 2 cut(s) 1095, 1386
PctI GAATGC 1 cut(s) 618
PdmI GAANNNNTTC 2 cut(s) 21, 705
PfeI GAWTC 2 cut(s) 1071, 1166
Pfl23II CGTACG 1 cut(s) 814
PinAI ACCGGT 1 cut(s) 824
PkrI GCNGC 2 cut(s) 205, 342
PluTI GGCGCC 1 cut(s) 837
Ppu21I YACGTR 1 cut(s) 814
PpuMI RGGWCCY 1 cut(s) 1025
PscI ACATGT 2 cut(s) 1095, 1386
PsiI TTATAA 1 cut(s) 1410
Psp124BI GAGCTC 2 cut(s) 489, 1314
Psp5II RGGWCCY 1 cut(s) 1025
Psp6I CCWGG 3 cut(s) 272, 687, 884
PspEI GGTNACC 1 cut(s) 935
PspGI CCWGG 3 cut(s) 272, 687, 884
PspLI CGTACG 1 cut(s) 814
PspN4I GGNNCC 2 cut(s) 835, 1027
PspPI GGNCC 5 cut(s) 512, 803, 1025, 1123, 1265
PspPPI RGGWCCY 1 cut(s) 1025
PstI CTGCAG 1 cut(s) 205
RsaI GTAC 2 cut(s) 224, 816
RsaNI GTAC 2 cut(s) 223, 815
RseI CAYNNNNRTG 5 cut(s) 494, 714, 794, 953, 1082
SacI GAGCTC 2 cut(s) 489, 1314
SaqAI TTAA 5 cut(s) 69, 87, 404, 642, 1293
SatI GCNGC 2 cut(s) 204, 341
Sau3AI GATC 4 cut(s) 42, 112, 217, 304
Sau96I GGNCC 5 cut(s) 512, 803, 1025, 1123, 1265
ScaI AGTACT 1 cut(s) 224
ScrFI CCNGG 5 cut(s) 214, 274, 459, 689, 886
SduI GDGCHC 5 cut(s) 135, 489, 729, 1115, 1314
SfaNI GCATC 2 cut(s) 465, 1006
SfcI CTRYAG 3 cut(s) 201, 981, 998
SfoI GGCGCC 1 cut(s) 835
SfuI TTCGAA 1 cut(s) 426
SinI GGWCC 2 cut(s) 1025, 1123
SmiMI CAYNNNNRTG 5 cut(s) 494, 714, 794, 953, 1082
SmlI CTYRAG 3 cut(s) 482, 854, 1365
SmoI CTYRAG 3 cut(s) 482, 854, 1365
SpeI ACTAGT 1 cut(s) 1457
Sse9I AATT 7 cut(s) 11, 61, 73, 411, 877, 929, 1354
SsiI CCGC 7 cut(s) 8, 434, 524, 712, 967, 1263, 1278
SspDI GGCGCC 1 cut(s) 833
SspMI CTAG 6 cut(s) 372, 584, 944, 1320, 1398, 1458
SstI GAGCTC 2 cut(s) 489, 1314
StyD4I CCNGG 5 cut(s) 212, 272, 457, 687, 884
StyI CCWWGG 1 cut(s) 549
TaaI ACNGT 3 cut(s) 34, 982, 1127
TaiI ACGT 1 cut(s) 816
TaqI TCGA 9 cut(s) 77, 153, 426, 560, 699, 735, 741, 1053, 1358
TasI AATT 7 cut(s) 11, 61, 73, 411, 877, 929, 1354
TatI WGTACW 1 cut(s) 222
TfiI GAWTC 2 cut(s) 1071, 1166
Tru1I TTAA 5 cut(s) 69, 87, 404, 642, 1293
Tru9I TTAA 5 cut(s) 69, 87, 404, 642, 1293
TscAI CASTG 5 cut(s) 128, 205, 332, 993, 1195
TseFI GTSAC 4 cut(s) 237, 716, 935, 1075
TseI GCWGC 2 cut(s) 203, 340
Tsp45I GTSAC 4 cut(s) 237, 716, 935, 1075
TspGWI ACGGA 2 cut(s) 73, 436
TspRI CASTG 5 cut(s) 128, 205, 332, 993, 1195
VneI GTGCAC 1 cut(s) 725
VpaK11BI GGWCC 2 cut(s) 1025, 1123
XapI RAATTY 4 cut(s) 11, 877, 929, 1354
XceI RCATGY 2 cut(s) 1099, 1390
XmnI GAANNNNTTC 2 cut(s) 21, 705
XspI CTAG 6 cut(s) 372, 584, 944, 1320, 1398, 1458
ZrmI AGTACT 1 cut(s) 224
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.