Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr8

Orthogroup ID

OG0008732

Physical Location & Seq

Genomic Coordinates Forward (+)

3635330 .. 3640508

Gene Structure 5179 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2031 bp

ATGTCTGAGGCGGTGGACGACGAGAGCGTGCGGAAGTTCGTGACGGCTTATTTGAAGAAGAAAGGCTTCAAGCAGGCGGAGAACGCTTTTCAAGAAGAACTAAACAAGAATACCAACAGCTCTTCGTCCCCGATCTCTTTCAATTCCCAATTGGACCCCGACGTCGCCAAGCACATCCTCTCCTTCTCCCAGTATGAGGAAGGTCCAGCACAGTATCAAGATGGATATGCCAAGTTGAGATCATGGACTTACACTTCACTAGATTTGTACAGGCATGAGTTGCTTCGCGTTCTCTATCCTGTATTTATTCATTGTTTCATGGATCTGGTTGCTAAAGGTCATATACAAGACGCTCGGACATTTTTCAATAGCTTCCGTGAAGACCATGAAATGATGCACCTACGAGATCTACAGAAGTTGGAAGGCGTTCTTTCACCCTCTCATTTGGAGGAGATGGAATTTGCTCATTCTCTTAGGCAGAGCAAAGTCAACATAAAGATATGCCAGTACTCGTATGAGCTTCTGCTGCAGTTTCTACACAAGTCACAATCCACCACAGTGCTTGGGATTATCAATGAGCATATTAACTTCCAAGTTTCTCCTGGACAACCCAGCTCAATTTCCGAAGATGCTGAGGCTGTAACACTTACCGGAAGTAGCCTGGATTCAGCTAATCAGATAAACCAGAAGGAAATACATTGGGGGTTGCTCGAAGATTCCTTTGAAGAACGCTTGGAAAAGGCTGGGGCCTTAGACTCTGAAAAAGCAGAAGGAGAAAGCAAAGAAGGGGATGGGGATGAGAATAAGAAAAAATCAAGTGAAGGAGCTAAACAAGGGTCTTCAAACAAAAAGTTGAAAAAGGACAAGGCTTCTGGTGCAACGGCGAAAACTGCACGCCCTGAGGCAACCCCTGTAACTGCAGTACCAAGAGTCAAACCAGAGCTTACTCTGCCAGTAATTCCCACAGAGGTTGAACAGTCAATCTTTGAAGACTTGAGAAACCGTGTACAATTGAGTAGTGCTGCACTGCCATCTGTCAGCTTTTATACATTCATCAACACACACAATGGTTTAAACTGTTCGTCTATATCCCATGATGGATCCATGGTTGCTGGTGGATTTTCAGACTCGTCGCTGAAGGTATGGGATATGGCAAAGATTGGGCAACAAGGCCTTGATTCAGCTTTGCAGGGTGAGAATGGTACCACCACTTCAAGTGAACAAGTTTTGTCAAATGGTGGAAAAAAACCATATACCTTGTTTCAGGGTCATTCGGGGCCAGTTTATTCAGCTACATTCAATCCCCTTGGGGATTTTATACTTTCCTCTTCGGCGGACTCAACTGTTCGGTTGTGGAGCACAAAATTAAATGCAAATCTTGTTTGCTACAAGGGTCATAATTACCCAGTATGGGATGTTCAGTTTAGCCCTGTCGGTCATTATTTTGCCAGTGCTTCACACGATCGAACAGCAAGGATTTGGTCTATGGACAGAATACAGCCTTTGAGAATAATGGCTGGGCACTTATCTGATGTTGATTGTGTGCAATGGCATGCCAACTGCAACTACGTTGCCACTGGCTCTAGTGATAAAACAGTTAGATTGTGGGACGTACAAACTGGAGAGTGTGTCCGAATATTTATTGGTCACAGGAGTATGGTTTTGTCTCTGGCAATGTCACCTGACGGTCGCTACATGGCATCTGGTGATGAAGACGGCACAATCATGATGTGGGACCTTTCAACTGGGCGCTGTGTTACACCTTTGACGGGTCACACCTCATGCGTATGGACACTTGATTTCAGTGGTGAGGGTTCGCTTCTTGCTTCTGGGTCTGCCGATTGTACTGTAAAACTATGGGACGTAACTGCAAGTACAAAGTTGCCAAGAACTGAAGAAAACAAAAGTGGAAATACCAGCAGACTCCGATCATTGAGGACTTTGCCGACCAAGTCTACTCCCGTCTACTCGTTACGGTTTTCTCGAAGAAACCTTCTATTTGCTTCTGGGGTTCTTTCAAAAACTGTATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000123 GO:0000228 GO:0000428 GO:0000785 GO:0000790 GO:0001067 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005667 GO:0005669 GO:0005694 GO:0005856 GO:0006139 GO:0006351 GO:0006352 GO:0006355 GO:0006366 GO:0006367 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009889 GO:0009987 GO:0010467 GO:0010468 GO:0010556 GO:0015629 GO:0016070 GO:0016591 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0030880 GO:0031248 GO:0031323 GO:0031326 GO:0031974 GO:0031981 GO:0032774 GO:0032991 GO:0033276 GO:0034641 GO:0034645 GO:0034654 GO:0042802 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044212 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044451 GO:0044454 GO:0044464 GO:0044798 GO:0046483 GO:0046983 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0055029 GO:0060255 GO:0061695 GO:0065007 GO:0070013 GO:0070461 GO:0071704 GO:0080090 GO:0090304 GO:0090575 GO:0097159 GO:0097659 GO:0140110 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1902493 GO:1902494 GO:1903506 GO:1990234 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

ko03022 ko05168 map03022 map05168

Pfam Domains

Protein Families

TFIID_NTD2 WD40

Protein Analysis

677

Amino Acids

74.82

Weight (kDa)

6.18

Isoelectric Point (pI)

47.24

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
TFIID_NTD2	PF04494	67 - 195	2e-45	WD40 associated region in TFIID subunit, NTD2 domain
Beta-prop_WDR5	PF25175	351 - 475	1e-11	WDR5 beta-propeller domain
Beta-prop_WDR3_1st	PF25173	352 - 538	6e-25	WDR3 first beta-propeller domain
Beta-prop_CAF1B_HIR1	PF24105	356 - 497	5.5e-10	CAF1B/HIR1 beta-propeller domain
WD40_Gbeta	PF25391	401 - 498	4.3e-07	G protein beta WD-40 repeat protein
WD40_CDC20-Fz	PF24807	402 - 544	1.6e-15	CDC20/Fizzy WD40 domain
Beta-prop_THOC3	PF25174	409 - 496	1.7e-15	THOC3 beta-propeller domain
Beta-prop_WDR36-Utp21_1st	PF25171	414 - 620	9.4e-13	WDR36/Utp21 first beta-propeller
WD40_Prp19	PF24814	414 - 580	3.1e-35	Prp19 WD40 domain
WD40	PF00400	416 - 453	7.6e-09	WD domain, G-beta repeat
WD40_WDHD1_1st	PF24817	416 - 455	4.5e-06	WDHD1 first WD40 domain
Beta-prop_TEP1_2nd	PF25047	416 - 580	4.5e-16	TEP-1 second beta-propeller
Beta-prop_WDR3_2nd	PF25172	417 - 537	9.6e-18	WDR3 second beta-propeller domain
Beta-prop_WDR36-Utp21_2nd	PF25168	420 - 489	3.4e-07	WDR36/Utp21 second beta-propeller domain
EIF3I	PF24805	420 - 498	1.6e-10	EIF3I
Beta-prop_IFT122_1st	PF23381	421 - 564	2.8e-07	IFT122 first beta-propeller
Beta-prop_EML_2	PF23414	426 - 525	4.1e-07	Echinoderm microtubule-associated protein second beta-propeller
WDR55	PF24796	428 - 584	1e-14	WDR55
WD40	PF00400	462 - 495	1e-08	WD domain, G-beta repeat
Beta-prop_WDR5	PF25175	464 - 627	2.8e-48	WDR5 beta-propeller domain
Beta-prop_THOC3	PF25174	466 - 545	7.7e-20	THOC3 beta-propeller domain
WDR90_beta-prop_4th	PF23342	470 - 593	5.4e-09	WDR90, 4th beta-propeller
WD40_MABP1-WDR62_2nd	PF24782	470 - 578	3.3e-14	MABP1/WDR62 second WD40 domain
WD40_CDC20-Fz	PF24807	473 - 629	1.5e-26	CDC20/Fizzy WD40 domain
Beta-prop_WDR19_1st	PF23389	478 - 627	2.2e-09	WDR19 first beta-propeller
Beta-prop_WDR90_POC16_2nd	PF23393	482 - 584	8.6e-13	WDR90/POC16, second beta-propeller
Beta-prop_WDR36-Utp21_2nd	PF25168	488 - 569	3.9e-10	WDR36/Utp21 second beta-propeller domain
Beta-prop_THOC3	PF25174	490 - 593	2.3e-24	THOC3 beta-propeller domain
WD40	PF00400	500 - 537	1.1e-09	WD domain, G-beta repeat
Beta-prop_CAF1B_HIR1	PF24105	502 - 586	1.7e-10	CAF1B/HIR1 beta-propeller domain
WD40_Gbeta	PF25391	503 - 628	8.4e-21	G protein beta WD-40 repeat protein
WD40_WDHD1_1st	PF24817	510 - 587	2.6e-15	WDHD1 first WD40 domain
Beta-prop_IFT140_1st	PF23383	510 - 580	2.4e-07	IFT140 first beta-propeller
Beta-prop_EML_2	PF23414	518 - 628	3.9e-16	Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_SCAP	PF24017	519 - 628	6.8e-08	SCAP Beta-propeller
Beta-prop_WDR3_1st	PF25173	532 - 634	3e-27	WDR3 first beta-propeller domain
Beta-prop_WDR3_2nd	PF25172	533 - 676	3.4e-15	WDR3 second beta-propeller domain
WD40	PF00400	541 - 579	6.7e-11	WD domain, G-beta repeat
Beta-prop_TEP1_2nd	PF25047	544 - 630	3.2e-09	TEP-1 second beta-propeller
Beta-prop_THOC3	PF25174	549 - 633	6.9e-20	THOC3 beta-propeller domain
Beta-prop_DCAF4	PF23761	550 - 626	5.6e-06	DDB1- and CUL4-associated factor 4 beta-propeller domain
Beta-prop_EML	PF23409	552 - 626	1.6e-06	Echinoderm microtubule-associated protein first beta-propeller
WD40_MABP1-WDR62_2nd	PF24782	554 - 640	2.5e-09	MABP1/WDR62 second WD40 domain
WD40_Prp19	PF24814	561 - 629	1.3e-14	Prp19 WD40 domain
Beta-prop_CAF1B_HIR1	PF24105	566 - 673	5.3e-07	CAF1B/HIR1 beta-propeller domain
Beta-prop_WDR36-Utp21_2nd	PF25168	571 - 626	2.2e-06	WDR36/Utp21 second beta-propeller domain
WD40	PF00400	583 - 621	3.5e-11	WD domain, G-beta repeat
EIF3I	PF24805	589 - 634	1e-05	EIF3I

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

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Genomic Context

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Orthologous Genes (Group: OG0008732)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G25150 AT5G25150
fragaria_vesca	FvH4_2g41020 FvH4_2g41020 FvH4_2g41020 FvH4_2g41020 FvH4_2g41020
malus_domestica	MD00G1143300.v1.1 MD15G1039100.v1.1
prunus_persica	Prupe.1G392800_v2.0.a1 Prupe.1G392800_v2.0.a1
pyrus_communis	pycom08g04780
rosa_chinensis	RchiOBHm_Chr6g0299541
rosa_laevigata	RLG00000011417
rosa_multiflora	Rmu_sc0004064.1_g000008
rosa_roxburghii	Rroxscaffold_7G00168570
rosa_rugosa	Rorug06G0289700 Rorug06G0289800 Rorug06G0289900 Rorug06G0289900
rosa_samantha	Rh6AG401100 Rh6DG402200
rosa_wichuraiana	Rw6G035100

Restriction Enzyme Sites

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Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	161
AatII	GACGTC	1 cut(s)	165
Acc65I	GGTACC	1 cut(s)	1202
AccB1I	GGYRCC	1 cut(s)	1202
AccI	GTMKAC	2 cut(s)	1955, 1965
AccII	CGCG	1 cut(s)	288
AciI	CCGC	4 cut(s)	11, 31, 77, 1334
AclWI	GGATC	3 cut(s)	330, 1095, 1108
AcsI	RAATTY	1 cut(s)	458
AcuI	CTGAAG	2 cut(s)	1157, 1914
AcyI	GRCGYC	1 cut(s)	162
AfaI	GTAC	8 cut(s)	269, 509, 924, 1008, 1204, 1614, 1846, 1876
AfiI	CCNNNNNNNGG	3 cut(s)	196, 1411, 1769
AjnI	CCWGG	2 cut(s)	601, 660
AleI	CACNNNNGTG	1 cut(s)	557
Alw21I	GWGCWC	1 cut(s)	1361
Alw26I	GTCTC	1 cut(s)	1671
AlwI	GGATC	3 cut(s)	330, 1095, 1108
AoxI	GGCC	3 cut(s)	747, 1171, 1277
ApeKI	GCWGC	2 cut(s)	526, 1022
ApoI	RAATTY	1 cut(s)	458
ArsI	GACNNNNNNTTYG	2 cut(s)	34, 66
Asp700I	GAANNNNTTC	2 cut(s)	65, 426
Asp718I	GGTACC	1 cut(s)	1202
AspLEI	GCGC	1 cut(s)	1752
AspS9I	GGNCC	5 cut(s)	154, 203, 747, 1277, 1735
AsuHPI	GGTGA	5 cut(s)	426, 1205, 1671, 1718, 1820
AvaII	GGWCC	3 cut(s)	154, 203, 1735
AxyI	CCTNAGG	1 cut(s)	900
BaeGI	GKGCMC	1 cut(s)	1524
BaeI	ACNNNNGTAYC	2 cut(s)	906, 939
BamHI	GGATCC	1 cut(s)	1100
BanI	GGYRCC	1 cut(s)	1202
BarI	GAAGNNNNNNTAC	2 cut(s)	1195, 1227
BbsI	GAAGAC	4 cut(s)	387, 831, 996, 1719
Bbv12I	GWGCWC	1 cut(s)	1361
BbvCI	CCTCAGC	1 cut(s)	633
BbvI	GCAGC	2 cut(s)	513, 1009
BccI	CCATC	5 cut(s)	215, 448, 785, 1039, 1091
BceAI	ACGGC	3 cut(s)	60, 897, 1732
BciT130I	CCWGG	2 cut(s)	603, 662
BcoDI	GTCTC	1 cut(s)	1671
BfaI	CTAG	2 cut(s)	260, 1584
BfmI	CTRYAG	3 cut(s)	410, 527, 918
BfoI	RGCGCY	1 cut(s)	1753
BglII	AGATCT	1 cut(s)	406
BisI	GCNGC	2 cut(s)	527, 1023
BlsI	GCNGC	2 cut(s)	528, 1024
BmcAI	AGTACT	1 cut(s)	509
Bme1390I	CCNGG	2 cut(s)	603, 662
Bme18I	GGWCC	3 cut(s)	154, 203, 1735
BmgT120I	GGNCC	5 cut(s)	154, 203, 747, 1277, 1735
BmiI	GGNNCC	6 cut(s)	156, 748, 1102, 1204, 1278, 1736
BmrFI	CCNGG	2 cut(s)	603, 662
BmrI	ACTGGG	3 cut(s)	184, 1400, 1755
BmsI	GCATC	3 cut(s)	384, 619, 1709
BmuI	ACTGGG	3 cut(s)	184, 1400, 1755
BpiI	GAAGAC	4 cut(s)	387, 831, 996, 1719
BpmI	CTGGAG	1 cut(s)	1641
Bpu10I	CCTNAGC	1 cut(s)	633
BpuEI	CTTGAG	1 cut(s)	1015
BsaHI	GRCGYC	1 cut(s)	162
BsaJI	CCNNGG	2 cut(s)	1104, 1306
BsaWI	WCCGGW	1 cut(s)	650
BsaXI	ACNNNNNCTCC	4 cut(s)	164, 194, 1614, 1644
Bsc4I	CCNNNNNNNGG	3 cut(s)	196, 1411, 1769
Bse1I	ACTGG	9 cut(s)	190, 505, 953, 1280, 1406, 1449, 1582, 1624, 1750
Bse21I	CCTNAGG	1 cut(s)	900
Bse3DI	GCAATG	2 cut(s)	1553, 1680
BseBI	CCWGG	2 cut(s)	603, 662
BseDI	CCNNGG	2 cut(s)	1104, 1306
BseGI	GGATG	4 cut(s)	174, 796, 802, 1420
BseLI	CCNNNNNNNGG	3 cut(s)	196, 1411, 1769
BseMI	GCAATG	2 cut(s)	1553, 1680
BseMII	CTCAG	2 cut(s)	624, 891
BseNI	ACTGG	9 cut(s)	190, 505, 953, 1280, 1406, 1449, 1582, 1624, 1750
BseRI	GAGGAG	1 cut(s)	464
BseSI	GKGCMC	1 cut(s)	1524
BseXI	GCAGC	2 cut(s)	513, 1009
BseYI	CCCAGC	3 cut(s)	611, 743, 1517
BsgI	GTGCAG	2 cut(s)	876, 1008
Bsh1236I	CGCG	1 cut(s)	288
Bsh1285I	CGRYCG	2 cut(s)	1465, 1690
BshFI	GGCC	3 cut(s)	749, 1173, 1279
BshNI	GGYRCC	1 cut(s)	1202
BsiEI	CGRYCG	2 cut(s)	1465, 1690
BsiHKAI	GWGCWC	1 cut(s)	1361
BsiSI	CCGG	1 cut(s)	651
BslFI	GGGAC	4 cut(s)	112, 1622, 1748, 1874
BslI	CCNNNNNNNGG	3 cut(s)	196, 1411, 1769
BsmAI	GTCTC	1 cut(s)	1671
BsmFI	GGGAC	4 cut(s)	112, 1622, 1748, 1874
BsnI	GGCC	3 cut(s)	749, 1173, 1279
Bsp1286I	GDGCHC	2 cut(s)	1361, 1524
Bsp1407I	TGTACA	2 cut(s)	267, 1006
Bsp143I	GATC	7 cut(s)	132, 239, 322, 406, 1100, 1462, 1928
Bsp19I	CCATGG	1 cut(s)	1104
BspACI	CCGC	4 cut(s)	11, 31, 77, 1334
BspANI	GGCC	3 cut(s)	749, 1173, 1279
BspCNI	CTCAG	2 cut(s)	625, 892
BspFNI	CGCG	1 cut(s)	288
BspHI	TCATGA	1 cut(s)	1725
BspLI	GGNNCC	6 cut(s)	156, 748, 1102, 1204, 1278, 1736
BspMAI	CTGCAG	2 cut(s)	531, 922
BspPI	GGATC	3 cut(s)	330, 1095, 1108
BspQI	GCTCTTC	1 cut(s)	127
BspT107I	GGYRCC	1 cut(s)	1202
BsrDI	GCAATG	2 cut(s)	1553, 1680
BsrGI	TGTACA	2 cut(s)	267, 1006
BsrI	ACTGG	9 cut(s)	190, 505, 953, 1280, 1406, 1449, 1582, 1624, 1750
BssECI	CCNNGG	2 cut(s)	1104, 1306
BssMI	GATC	7 cut(s)	132, 239, 322, 406, 1100, 1462, 1928
BssNI	GRCGYC	1 cut(s)	162
BssT1I	CCWWGG	2 cut(s)	1104, 1306
Bst2UI	CCWGG	2 cut(s)	603, 662
Bst6I	CTCTTC	2 cut(s)	127, 1333
BstACI	GRCGYC	1 cut(s)	162
BstAPI	GCANNNNNTGC	1 cut(s)	280
BstAUI	TGTACA	2 cut(s)	267, 1006
BstC8I	GCNNGC	4 cut(s)	29, 75, 895, 1554
BstDEI	CTNAG	5 cut(s)	6, 473, 633, 751, 900
BstDSI	CCRYGG	1 cut(s)	1104
BstF5I	GGATG	4 cut(s)	174, 796, 802, 1420
BstFNI	CGCG	1 cut(s)	288
BstH2I	RGCGCY	1 cut(s)	1753
BstHHI	GCGC	1 cut(s)	1752
BstKTI	GATC	7 cut(s)	135, 242, 325, 409, 1103, 1465, 1931
BstMAI	GTCTC	1 cut(s)	1671
BstMBI	GATC	7 cut(s)	132, 239, 322, 406, 1100, 1462, 1928
BstMCI	CGRYCG	2 cut(s)	1465, 1690
BstMWI	GCNNNNNNNGC	6 cut(s)	83, 280, 526, 875, 890, 949
BstNI	CCWGG	2 cut(s)	603, 662
BstNSI	RCATGY	1 cut(s)	1556
BstSCI	CCNGG	2 cut(s)	601, 660
BstSFI	CTRYAG	3 cut(s)	410, 527, 918
BstSLI	GKGCMC	1 cut(s)	1524
BstUI	CGCG	1 cut(s)	288
BstV1I	GCAGC	2 cut(s)	513, 1009
BstV2I	GAAGAC	4 cut(s)	387, 831, 996, 1719
BstX2I	RGATCY	3 cut(s)	322, 406, 1100
BstYI	RGATCY	3 cut(s)	322, 406, 1100
Bsu36I	CCTNAGG	1 cut(s)	900
BsuRI	GGCC	3 cut(s)	749, 1173, 1279
BtgI	CCRYGG	1 cut(s)	1104
BtsCI	GGATG	4 cut(s)	174, 796, 802, 1420
BtsI	GCAGTG	1 cut(s)	1025
BtsIMutI	CAGTG	5 cut(s)	564, 1025, 1456, 1575, 1810
Cac8I	GCNNGC	4 cut(s)	29, 75, 895, 1554
CciI	TCATGA	1 cut(s)	1725
CfoI	GCGC	1 cut(s)	1752
Cfr13I	GGNCC	5 cut(s)	154, 203, 747, 1277, 1735
CseI	GACGC	1 cut(s)	359
Csp6I	GTAC	8 cut(s)	268, 508, 923, 1007, 1203, 1613, 1845, 1875
CspCI	CAANNNNNGTGG	4 cut(s)	544, 579, 952, 987
CviQI	GTAC	8 cut(s)	268, 508, 923, 1007, 1203, 1613, 1845, 1875
DdeI	CTNAG	5 cut(s)	6, 473, 633, 751, 900
DpnI	GATC	7 cut(s)	134, 241, 324, 408, 1102, 1464, 1930
DpnII	GATC	7 cut(s)	132, 239, 322, 406, 1100, 1462, 1928
DraI	TTTAAA	1 cut(s)	1074
DrdI	GACNNNNNNGTC	1 cut(s)	161
DseDI	GACNNNNNNGTC	1 cut(s)	161
Eam1104I	CTCTTC	2 cut(s)	127, 1333
EarI	CTCTTC	2 cut(s)	127, 1333
EciI	GGCGGA	2 cut(s)	92, 1349
Eco130I	CCWWGG	2 cut(s)	1104, 1306
Eco147I	AGGCCT	1 cut(s)	1173
Eco47I	GGWCC	3 cut(s)	154, 203, 1735
Eco57I	CTGAAG	2 cut(s)	1157, 1914
Eco81I	CCTNAGG	1 cut(s)	900
EcoO109I	RGGNCCY	2 cut(s)	747, 1735
EcoRII	CCWGG	2 cut(s)	601, 660
EcoT14I	CCWWGG	2 cut(s)	1104, 1306
ErhI	CCWWGG	2 cut(s)	1104, 1306
FalI	AAGNNNNNCTT	4 cut(s)	50, 82, 414, 446
FaqI	GGGAC	4 cut(s)	112, 1622, 1748, 1874
FblI	GTMKAC	2 cut(s)	1955, 1965
Fnu4HI	GCNGC	2 cut(s)	527, 1023
FokI	GGATG	4 cut(s)	161, 803, 809, 1427
Fsp4HI	GCNGC	2 cut(s)	527, 1023
FspBI	CTAG	2 cut(s)	260, 1584
GlaI	GCGC	1 cut(s)	1751
GluI	GCNGC	2 cut(s)	527, 1023
GsaI	CCCAGC	3 cut(s)	615, 747, 1521
GsuI	CTGGAG	1 cut(s)	1641
HaeII	RGCGCY	1 cut(s)	1753
HaeIII	GGCC	3 cut(s)	749, 1173, 1279
HapII	CCGG	1 cut(s)	651
HgaI	GACGC	1 cut(s)	359
HhaI	GCGC	1 cut(s)	1752
Hin1I	GRCGYC	1 cut(s)	162
Hin6I	GCGC	1 cut(s)	1750
HinP1I	GCGC	1 cut(s)	1750
HincII	GTYRAC	1 cut(s)	490
HindII	GTYRAC	1 cut(s)	490
HinfI	GANTC	8 cut(s)	665, 716, 755, 930, 1127, 1178, 1337, 1923
HpaII	CCGG	1 cut(s)	651
HphI	GGTGA	5 cut(s)	426, 1205, 1671, 1718, 1820
Hpy166II	GTNNAC	6 cut(s)	16, 490, 1007, 1220, 1956, 1966
Hpy188I	TCNGA	9 cut(s)	7, 357, 625, 678, 760, 1126, 1531, 1634, 1928
Hpy188III	TCNNGA	5 cut(s)	40, 92, 218, 1726, 1983
Hpy8I	GTNNAC	6 cut(s)	16, 490, 1007, 1220, 1956, 1966
Hpy99I	CGWCG	4 cut(s)	23, 164, 167, 1135
HpyAV	CCTTC	9 cut(s)	193, 194, 416, 682, 764, 779, 815, 1132, 2003
HpyCH4IV	ACGT	4 cut(s)	162, 1569, 1611, 1863
HpyF10VI	GCNNNNNNNGC	6 cut(s)	83, 280, 526, 875, 890, 949
HpyF3I	CTNAG	5 cut(s)	6, 473, 633, 751, 900
HpySE526I	ACGT	4 cut(s)	162, 1569, 1611, 1863
Hsp92I	GRCGYC	1 cut(s)	162
HspAI	GCGC	1 cut(s)	1750
KpnI	GGTACC	1 cut(s)	1206
Kzo9I	GATC	7 cut(s)	132, 239, 322, 406, 1100, 1462, 1928
LguI	GCTCTTC	1 cut(s)	127
LmnI	GCTCC	2 cut(s)	824, 1356
Lsp1109I	GCAGC	2 cut(s)	513, 1009
LweI	GCATC	3 cut(s)	384, 619, 1709
MaeI	CTAG	2 cut(s)	260, 1584
MaeII	ACGT	4 cut(s)	162, 1569, 1611, 1863
MalI	GATC	7 cut(s)	134, 241, 324, 408, 1102, 1464, 1930
MboI	GATC	7 cut(s)	132, 239, 322, 406, 1100, 1462, 1928
MfeI	CAATTG	2 cut(s)	149, 1010
MflI	RGATCY	3 cut(s)	322, 406, 1100
MhlI	GDGCHC	2 cut(s)	1361, 1524
MluCI	AATT	8 cut(s)	142, 149, 458, 618, 957, 1010, 1364, 1399
MlyI	GAGTC	5 cut(s)	749, 939, 1121, 1331, 1917
MmeI	TCCRAC	1 cut(s)	399
MroXI	GAANNNNTTC	2 cut(s)	65, 426
MseI	TTAA	3 cut(s)	585, 1073, 1367
MslI	CAYNNNNRTG	3 cut(s)	557, 1724, 1786
MspI	CCGG	1 cut(s)	651
MspR9I	CCNGG	2 cut(s)	603, 662
MssI	GTTTAAAC	1 cut(s)	1074
MunI	CAATTG	2 cut(s)	149, 1010
MvaI	CCWGG	2 cut(s)	603, 662
MvnI	CGCG	1 cut(s)	288
MwoI	GCNNNNNNNGC	6 cut(s)	83, 280, 526, 875, 890, 949
NcoI	CCATGG	1 cut(s)	1104
NdeII	GATC	7 cut(s)	132, 239, 322, 406, 1100, 1462, 1928
NlaIV	GGNNCC	6 cut(s)	156, 748, 1102, 1204, 1278, 1736
NmuCI	GTSAC	5 cut(s)	40, 543, 1646, 1677, 1772
NspI	RCATGY	1 cut(s)	1556
OliI	CACNNNNGTG	1 cut(s)	557
PaeI	GCATGC	1 cut(s)	1556
PagI	TCATGA	1 cut(s)	1725
PceI	AGGCCT	1 cut(s)	1173
PciSI	GCTCTTC	1 cut(s)	127
PcsI	WCGNNNNNNNCGW	2 cut(s)	24, 1981
PdmI	GAANNNNTTC	2 cut(s)	65, 426
PfeI	GAWTC	3 cut(s)	665, 716, 1178
PflFI	GACNNNGTC	1 cut(s)	1951
PfoI	TCCNGGA	1 cut(s)	601
PkrI	GCNGC	2 cut(s)	528, 1024
Ple19I	CGATCG	1 cut(s)	1465
PleI	GAGTC	5 cut(s)	749, 938, 1121, 1331, 1917
PmeI	GTTTAAAC	1 cut(s)	1074
PpsI	GAGTC	5 cut(s)	749, 938, 1121, 1331, 1917
PpuMI	RGGWCCY	1 cut(s)	1735
Psp5II	RGGWCCY	1 cut(s)	1735
Psp6I	CCWGG	2 cut(s)	601, 660
PspFI	CCCAGC	3 cut(s)	611, 743, 1517
PspGI	CCWGG	2 cut(s)	601, 660
PspN4I	GGNNCC	6 cut(s)	156, 748, 1102, 1204, 1278, 1736
PspPI	GGNCC	5 cut(s)	154, 203, 747, 1277, 1735
PspPPI	RGGWCCY	1 cut(s)	1735
PsrI	GAACNNNNNNTAC	2 cut(s)	1401, 1433
PstI	CTGCAG	2 cut(s)	531, 922
PsuI	RGATCY	3 cut(s)	322, 406, 1100
PsyI	GACNNNGTC	1 cut(s)	1951
PvuI	CGATCG	1 cut(s)	1465
RsaI	GTAC	8 cut(s)	269, 509, 924, 1008, 1204, 1614, 1846, 1876
RsaNI	GTAC	8 cut(s)	268, 508, 923, 1007, 1203, 1613, 1845, 1875
RseI	CAYNNNNRTG	3 cut(s)	557, 1724, 1786
SapI	GCTCTTC	1 cut(s)	127
SaqAI	TTAA	3 cut(s)	585, 1073, 1367
SatI	GCNGC	2 cut(s)	527, 1023
Sau3AI	GATC	7 cut(s)	132, 239, 322, 406, 1100, 1462, 1928
Sau96I	GGNCC	5 cut(s)	154, 203, 747, 1277, 1735
ScaI	AGTACT	1 cut(s)	509
SchI	GAGTC	5 cut(s)	749, 939, 1121, 1331, 1917
ScrFI	CCNGG	2 cut(s)	603, 662
SduI	GDGCHC	2 cut(s)	1361, 1524
SfaNI	GCATC	3 cut(s)	384, 619, 1709
SfcI	CTRYAG	3 cut(s)	410, 527, 918
SinI	GGWCC	3 cut(s)	154, 203, 1735
SmiMI	CAYNNNNRTG	3 cut(s)	557, 1724, 1786
SmlI	CTYRAG	1 cut(s)	994
SmoI	CTYRAG	1 cut(s)	994
SphI	GCATGC	1 cut(s)	1556
Sse9I	AATT	8 cut(s)	142, 149, 458, 618, 957, 1010, 1364, 1399
SseBI	AGGCCT	1 cut(s)	1173
SsiI	CCGC	4 cut(s)	11, 31, 77, 1334
SspI	AATATT	1 cut(s)	1638
SspMI	CTAG	2 cut(s)	260, 1584
StuI	AGGCCT	1 cut(s)	1173
StyD4I	CCNGG	2 cut(s)	601, 660
StyI	CCWWGG	2 cut(s)	1104, 1306
TaiI	ACGT	4 cut(s)	165, 1572, 1614, 1866
TaqI	TCGA	3 cut(s)	711, 1465, 1984
TaqII	GACCGA	1 cut(s)	1424
TasI	AATT	8 cut(s)	142, 149, 458, 618, 957, 1010, 1364, 1399
TatI	WGTACW	5 cut(s)	267, 507, 1006, 1844, 1874
TfiI	GAWTC	3 cut(s)	665, 716, 1178
Tru1I	TTAA	3 cut(s)	585, 1073, 1367
Tru9I	TTAA	3 cut(s)	585, 1073, 1367
TscAI	CASTG	5 cut(s)	564, 1032, 1456, 1582, 1810
TseFI	GTSAC	5 cut(s)	40, 543, 1646, 1677, 1772
TseI	GCWGC	2 cut(s)	526, 1022
Tsp45I	GTSAC	5 cut(s)	40, 543, 1646, 1677, 1772
TspDTI	ATGAA	5 cut(s)	299, 307, 402, 1042, 1725
TspGWI	ACGGA	1 cut(s)	365
TspRI	CASTG	5 cut(s)	564, 1032, 1456, 1582, 1810
Tth111I	GACNNNGTC	1 cut(s)	1951
VpaK11BI	GGWCC	3 cut(s)	154, 203, 1735
XapI	RAATTY	1 cut(s)	458
XceI	RCATGY	1 cut(s)	1556
XcmI	CCANNNNNNNNNTGG	1 cut(s)	599
XmiI	GTMKAC	2 cut(s)	1955, 1965
XmnI	GAANNNNTTC	2 cut(s)	65, 426
XspI	CTAG	2 cut(s)	260, 1584
ZraI	GACGTC	1 cut(s)	163
ZrmI	AGTACT	1 cut(s)	509

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.