pycom08g05060

Protein REVERSION-TO-ETHYLENE SENSITIVITY1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0004008
Physical Location & Seq
Reverse (-)
3880046 .. 3880933
888 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 567 bp
ATGCGGCACTTTAGATTTCGTGGGATGGAGATGAAAGAAGCGTATGACATCGAGCATTTGACCTCTACGCAGAATATTGAGCATGAGCTGTGGCCTCTCACTGAAATTGATCCGAAGAAGGCAAAGTTTCCCTGCTGTATAGTTTGGACTCCGCTTCCGGTAGTCTCCTGGTTGGCACCATTCATCGGACATGTTGGCATTTGCAGGGAGGATGGAGCTATTTTAGATTTCTCTGGTTCCAATTTTGTGAATGTTGATGACTTTGCATTTGGTGCCGTAGCCAGATATCTCCAAATTGACCGAAAACAGTGTTGTTTTCCCGCAAATCTGGGTAGCCACATTTGCAAGCATGGTTACAAGCATTCAGAGTTTGGGACTGCATTAACATGGGATGATGCCTTGCAGTCAAGTTCGCGGTACTTTGAACATAAAACCTACAACCTCTTCACTTGCAACTGCCATTCATTTGTAGCCAACTGTCTCAATCGGCTCTGCTATGGCGGATCAATGAGTTGGAACATCGATCAACGTGGCAGGCTTAGTACTGCTCAAGGGGCATTGGGTTGA

Protein Analysis

189

Amino Acids

21.39

Weight (kDa)

6.73

Isoelectric Point (pI)

41.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RTE1 PF05608 37 - 174 4.2e-70 RTE1-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 175, 272
AccII CGCG 1 cut(s) 415
AciI CCGC 5 cut(s) 4, 152, 321, 415, 501
AclWI GGATC 2 cut(s) 104, 511
AfaI GTAC 2 cut(s) 419, 544
AfiI CCNNNNNNNGG 1 cut(s) 185
AflIII ACRYGT 1 cut(s) 190
AgsI TTSAA 1 cut(s) 425
AjnI CCWGG 1 cut(s) 167
AluBI AGCT 2 cut(s) 88, 218
AluI AGCT 2 cut(s) 88, 218
Alw26I GTCTC 2 cut(s) 169, 485
AlwI GGATC 2 cut(s) 104, 511
AoxI GGCC 1 cut(s) 92
ArsI GACNNNNNNTTYG 2 cut(s) 251, 283
BanI GGYRCC 2 cut(s) 175, 272
BccI CCATC 2 cut(s) 19, 206
BceAI ACGGC 1 cut(s) 260
BciT130I CCWGG 1 cut(s) 169
BcoDI GTCTC 2 cut(s) 169, 485
BisI GCNGC 1 cut(s) 5
BlsI GCNGC 1 cut(s) 6
BmcAI AGTACT 1 cut(s) 544
Bme1390I CCNGG 1 cut(s) 169
BmiI GGNNCC 3 cut(s) 177, 238, 274
BmrFI CCNGG 1 cut(s) 169
BmsI GCATC 1 cut(s) 385
BpuEI CTTGAG 1 cut(s) 534
Bsa29I ATCGAT 1 cut(s) 522
BsaWI WCCGGW 1 cut(s) 157
Bsc4I CCNNNNNNNGG 1 cut(s) 185
BseBI CCWGG 1 cut(s) 169
BseCI ATCGAT 1 cut(s) 522
BseGI GGATG 3 cut(s) 30, 217, 397
BseLI CCNNNNNNNGG 1 cut(s) 185
Bsh1236I CGCG 1 cut(s) 415
BshFI GGCC 1 cut(s) 94
BshNI GGYRCC 2 cut(s) 175, 272
BshVI ATCGAT 1 cut(s) 522
BsiSI CCGG 1 cut(s) 158
BslFI GGGAC 1 cut(s) 388
BslI CCNNNNNNNGG 1 cut(s) 185
BsmAI GTCTC 2 cut(s) 169, 485
BsmFI GGGAC 1 cut(s) 388
BsmI GAATGC 1 cut(s) 361
BsnI GGCC 1 cut(s) 94
Bsp143I GATC 3 cut(s) 109, 503, 523
BspACI CCGC 5 cut(s) 4, 152, 321, 415, 501
BspANI GGCC 1 cut(s) 94
BspDI ATCGAT 1 cut(s) 522
BspFNI CGCG 1 cut(s) 415
BspLI GGNNCC 3 cut(s) 177, 238, 274
BspPI GGATC 2 cut(s) 104, 511
BspT107I GGYRCC 2 cut(s) 175, 272
BssMI GATC 3 cut(s) 109, 503, 523
Bst2UI CCWGG 1 cut(s) 169
Bst4CI ACNGT 2 cut(s) 309, 479
Bst6I CTCTTC 1 cut(s) 449
BstAPI GCANNNNNTGC 1 cut(s) 272
BstC8I GCNNGC 2 cut(s) 347, 536
BstDEI CTNAG 1 cut(s) 539
BstF5I GGATG 3 cut(s) 30, 217, 397
BstFNI CGCG 1 cut(s) 415
BstKTI GATC 3 cut(s) 112, 506, 526
BstMAI GTCTC 2 cut(s) 169, 485
BstMBI GATC 3 cut(s) 109, 503, 523
BstMWI GCNNNNNNNGC 3 cut(s) 272, 342, 554
BstNI CCWGG 1 cut(s) 169
BstNSI RCATGY 1 cut(s) 194
BstSCI CCNGG 1 cut(s) 167
BstUI CGCG 1 cut(s) 415
Bsu15I ATCGAT 1 cut(s) 522
BsuRI GGCC 1 cut(s) 94
BsuTUI ATCGAT 1 cut(s) 522
BtsCI GGATG 3 cut(s) 30, 217, 397
BtsIMutI CAGTG 2 cut(s) 99, 314
Cac8I GCNNGC 2 cut(s) 347, 536
ClaI ATCGAT 1 cut(s) 522
Csp6I GTAC 2 cut(s) 418, 543
CviAII CATG 4 cut(s) 83, 191, 350, 387
CviJI RGCY 8 cut(s) 88, 94, 218, 281, 336, 473, 490, 538
CviKI_1 RGCY 8 cut(s) 88, 94, 218, 281, 336, 473, 490, 538
CviQI GTAC 2 cut(s) 418, 543
DdeI CTNAG 1 cut(s) 539
DpnI GATC 3 cut(s) 111, 505, 525
DpnII GATC 3 cut(s) 109, 503, 523
Eam1104I CTCTTC 1 cut(s) 449
EarI CTCTTC 1 cut(s) 449
EciI GGCGGA 1 cut(s) 516
Eco32I GATATC 1 cut(s) 287
EcoRII CCWGG 1 cut(s) 167
EcoRV GATATC 1 cut(s) 287
FaeI CATG 4 cut(s) 86, 194, 353, 390
FaiI YATR 8 cut(s) 45, 84, 140, 192, 351, 388, 429, 498
FaqI GGGAC 1 cut(s) 388
FatI CATG 4 cut(s) 82, 190, 349, 386
FauI CCCGC 1 cut(s) 328
Fnu4HI GCNGC 1 cut(s) 5
FokI GGATG 3 cut(s) 37, 224, 404
Fsp4HI GCNGC 1 cut(s) 5
GluI GCNGC 1 cut(s) 5
HaeIII GGCC 1 cut(s) 94
HapII CCGG 1 cut(s) 158
Hin1II CATG 4 cut(s) 86, 194, 353, 390
HinfI GANTC 1 cut(s) 148
HpaII CCGG 1 cut(s) 158
Hpy188I TCNGA 3 cut(s) 114, 188, 367
HpyAV CCTTC 1 cut(s) 112
HpyCH4III ACNGT 2 cut(s) 309, 479
HpyCH4IV ACGT 1 cut(s) 529
HpyCH4V TGCA 6 cut(s) 204, 266, 345, 380, 403, 453
HpyF10VI GCNNNNNNNGC 3 cut(s) 272, 342, 554
HpyF3I CTNAG 1 cut(s) 539
HpySE526I ACGT 1 cut(s) 529
Hsp92II CATG 4 cut(s) 86, 194, 353, 390
Kzo9I GATC 3 cut(s) 109, 503, 523
LmnI GCTCC 1 cut(s) 215
LpnPI CCDG 9 cut(s) 145, 154, 171, 181, 190, 219, 295, 314, 520
LweI GCATC 1 cut(s) 385
MaeII ACGT 1 cut(s) 529
MaeIII GTNAC 1 cut(s) 353
MalI GATC 3 cut(s) 111, 505, 525
MboI GATC 3 cut(s) 109, 503, 523
MboII GAAGA 2 cut(s) 127, 436
MluCI AATT 3 cut(s) 105, 241, 294
MlyI GAGTC 1 cut(s) 142
MmeI TCCRAC 1 cut(s) 494
MnlI CCTC 4 cut(s) 73, 105, 202, 452
MseI TTAA 1 cut(s) 383
MslI CAYNNNNRTG 1 cut(s) 385
MspI CCGG 1 cut(s) 158
MspR9I CCNGG 1 cut(s) 169
Mva1269I GAATGC 1 cut(s) 361
MvaI CCWGG 1 cut(s) 169
MvnI CGCG 1 cut(s) 415
MwoI GCNNNNNNNGC 3 cut(s) 272, 342, 554
NdeII GATC 3 cut(s) 109, 503, 523
NlaIII CATG 4 cut(s) 86, 194, 353, 390
NlaIV GGNNCC 3 cut(s) 177, 238, 274
NspI RCATGY 1 cut(s) 194
PciI ACATGT 1 cut(s) 190
PctI GAATGC 1 cut(s) 361
PkrI GCNGC 1 cut(s) 6
PleI GAGTC 1 cut(s) 142
PpsI GAGTC 1 cut(s) 142
PscI ACATGT 1 cut(s) 190
Psp6I CCWGG 1 cut(s) 167
PspGI CCWGG 1 cut(s) 167
PspN4I GGNNCC 3 cut(s) 177, 238, 274
RsaI GTAC 2 cut(s) 419, 544
RsaNI GTAC 2 cut(s) 418, 543
RseI CAYNNNNRTG 1 cut(s) 385
SaqAI TTAA 1 cut(s) 383
SatI GCNGC 1 cut(s) 5
Sau3AI GATC 3 cut(s) 109, 503, 523
ScaI AGTACT 1 cut(s) 544
SchI GAGTC 1 cut(s) 142
ScrFI CCNGG 1 cut(s) 169
SetI ASST 6 cut(s) 65, 90, 220, 437, 444, 532
SfaNI GCATC 1 cut(s) 385
SmiMI CAYNNNNRTG 1 cut(s) 385
SmlI CTYRAG 1 cut(s) 549
SmoI CTYRAG 1 cut(s) 549
Sse9I AATT 3 cut(s) 105, 241, 294
SsiI CCGC 5 cut(s) 4, 152, 321, 415, 501
SspI AATATT 1 cut(s) 76
StyD4I CCNGG 1 cut(s) 167
TaaI ACNGT 2 cut(s) 309, 479
TaiI ACGT 1 cut(s) 532
TaqI TCGA 2 cut(s) 51, 522
TaqII GACCGA 1 cut(s) 315
TasI AATT 3 cut(s) 105, 241, 294
TatI WGTACW 1 cut(s) 542
TauI GCSGC 1 cut(s) 7
Tru1I TTAA 1 cut(s) 383
Tru9I TTAA 1 cut(s) 383
TscAI CASTG 2 cut(s) 106, 314
TspDTI ATGAA 3 cut(s) 47, 172, 453
TspRI CASTG 2 cut(s) 106, 314
XceI RCATGY 1 cut(s) 194
ZrmI AGTACT 1 cut(s) 544
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.