pycom08g08410

BRI1 kinase inhibitor 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0012335
Physical Location & Seq
Reverse (-)
6739284 .. 6740264
981 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g08410.1

Sequence Viewer

Length: 981 bp
ATGGCCACCCATCAGCTGGAAAAAACCAGAGAAATTGCTCTAGACACCCTCAGCAGGCATGAAGAAGAAAGGTTAAAACAGTTACCAAAAACTGCCCCTGCCTCACCACCTTCATCTTCCTCCTCCCCCTCCCATGAATTCTCCTTCACAATCTCTCTCCACACTTCTTCTTCTTCCACGTCAAACCCTAATCACCAATCCAAATCCAAAACCAGCACCACCCCACCTTCTTTCGCCATTGATTTGTCCCCCGCAGATGAAATATTCTTCCACGGCCACCTCCTCCCCCTCCAGTTTCTCTCCCATCTCCCCGTCTCTCCTCGCTCCTCCACCAACTCCCTCGACAGCTTCACCCTCCCCCTCAAAGAGCTGGTAGAAGATCAAAAACCAACCAGAAAAACCGCCACAAACAATGCCGAAAACTGCATTCGAAACCGAAACATCCTAAACAAGAAGAACAACAGCTGCAGCAGCATCGACAAGAGAATCCCACGTCAGGGAAGCAAACACGAGACCAGGGGAAGAACAAAGTCCAAGTCCTTTTCGATTTTCGGGCTTCCTAGATGGCGAAAATATTGTGAAAATAGCGAAAAGGAGGAGAACAACAAGGAGATGAAGAACAAGAGGAAGGTAAGGTTTGATGTGAGTCACATACTGAAGAGGTACGTGAGAATGGTGAGGCCGCTGTTGTTGTTTAGAGGGAGGAGAGAGAAGGGGCAGTTCCGGCGGCAGCCCTATTCGTTTTCGGGGAATTTGAGCATGTTAAACAAACATGAGTTGAGAGGAAGGATGGGACAGTTTTCGGCGCCGGCGTCAATGAGGACATCTCCGACGAATAGTGGGCATCTTGTTGCAACTGCAGCTACTAATGTTCCTTGTTCTTCAAGTGACAGCACCATGGAAGAGTTGCAGGCTGCAATTCAAGCTGCAATTGCTCATTGCAAGAACTCCATTGCCACAGAAGAGAAGCCCAAATGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
No domains found.

Protein Analysis

327

Amino Acids

36.72

Weight (kDa)

10.04

Isoelectric Point (pI)

60.72

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012335)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G42750
fragaria_vesca FvH4_2g34560
malus_domestica MD08G1102600.v1.1 MD15G1084100.v1.1
prunus_persica Prupe.1G437500_v2.0.a1
pyrus_communis pycom08g08410 pycom15g07930
rosa_chinensis RchiOBHm_Chr6g0307601 RchiOBHm_Chr6g0307761
rosa_laevigata RLG00000010702
rosa_multiflora Rmu_co8485591.1_g000001 Rmu_sc0000031.1_g000029
rosa_roxburghii Rroxscaffold_7G00160910
rosa_rugosa Rorug06G0360600
rosa_samantha Rh6AG469700 Rh6BG480200 Rh6CG485900 Rh6DG472400
rosa_wichuraiana Rw6G041010

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 805
AccB7I CCANNNNNTGG 1 cut(s) 16
AciI CCGC 4 cut(s) 252, 402, 683, 727
AcoI YGGCCR 2 cut(s) 3, 274
AcsI RAATTY 2 cut(s) 137, 751
AcuI CTGAAG 1 cut(s) 677
AcyI GRCGYC 2 cut(s) 806, 812
AfaI GTAC 1 cut(s) 665
AfiI CCNNNNNNNGG 4 cut(s) 16, 54, 496, 497
AgsI TTSAA 2 cut(s) 885, 923
AjiI CACGTC 2 cut(s) 180, 494
AjnI CCWGG 1 cut(s) 515
AluBI AGCT 6 cut(s) 16, 348, 370, 465, 863, 926
AluI AGCT 6 cut(s) 16, 348, 370, 465, 863, 926
Alw26I GTCTC 2 cut(s) 319, 506
AoxI GGCC 3 cut(s) 3, 274, 680
ApeKI GCWGC 7 cut(s) 465, 468, 471, 730, 860, 914, 926
ApoI RAATTY 2 cut(s) 137, 751
ArsI GACNNNNNNTTYG 2 cut(s) 521, 553
AspLEI GCGC 1 cut(s) 808
AsuHPI GGTGA 4 cut(s) 96, 185, 343, 688
AsuII TTCGAA 1 cut(s) 430
BalI TGGCCA 1 cut(s) 5
BanI GGYRCC 1 cut(s) 805
BauI CACGAG 1 cut(s) 509
BbvCI CCTCAGC 1 cut(s) 50
BbvI GCAGC 7 cut(s) 452, 480, 483, 742, 872, 901, 913
BccI CCATC 4 cut(s) 18, 312, 558, 784
BceAI ACGGC 1 cut(s) 289
BcgI CGANNNNNNTGC 2 cut(s) 457, 491
BciT130I CCWGG 1 cut(s) 517
BcoDI GTCTC 2 cut(s) 319, 506
BfaI CTAG 2 cut(s) 41, 561
BfmI CTRYAG 2 cut(s) 466, 858
BfoI RGCGCY 1 cut(s) 809
BisI GCNGC 9 cut(s) 466, 469, 472, 683, 728, 731, 861, 915, 927
BlsI GCNGC 9 cut(s) 467, 470, 473, 684, 729, 732, 862, 916, 928
Bme1390I CCNGG 1 cut(s) 517
BmgBI CACGTC 2 cut(s) 180, 494
BmiI GGNNCC 1 cut(s) 807
BmrFI CCNGG 1 cut(s) 517
BmsI GCATC 2 cut(s) 483, 853
BplI GAGNNNNNCTC 2 cut(s) 811, 843
BpmI CTGGAG 1 cut(s) 275
Bpu10I CCTNAGC 1 cut(s) 50
Bpu14I TTCGAA 1 cut(s) 430
BsaAI YACGTR 1 cut(s) 667
BsaHI GRCGYC 2 cut(s) 806, 812
BsaI GGTCTC 1 cut(s) 506
BsaJI CCNNGG 3 cut(s) 271, 516, 897
Bsc4I CCNNNNNNNGG 4 cut(s) 16, 54, 496, 497
Bse118I RCCGGY 1 cut(s) 808
Bse1I ACTGG 1 cut(s) 292
Bse3DI GCAATG 2 cut(s) 937, 951
BseBI CCWGG 1 cut(s) 517
BseDI CCNNGG 3 cut(s) 271, 516, 897
BseGI GGATG 2 cut(s) 441, 795
BseLI CCNNNNNNNGG 4 cut(s) 16, 54, 496, 497
BseMI GCAATG 2 cut(s) 937, 951
BseMII CTCAG 1 cut(s) 64
BseNI ACTGG 1 cut(s) 292
BseRI GAGGAG 6 cut(s) 112, 272, 309, 316, 611, 718
BseXI GCAGC 7 cut(s) 452, 480, 483, 742, 872, 901, 913
BshFI GGCC 3 cut(s) 5, 276, 682
BshNI GGYRCC 1 cut(s) 805
BsiSI CCGG 2 cut(s) 724, 809
BslFI GGGAC 2 cut(s) 232, 807
BslI CCNNNNNNNGG 4 cut(s) 16, 54, 496, 497
BsmAI GTCTC 2 cut(s) 319, 506
BsmBI CGTCTC 1 cut(s) 319
BsmFI GGGAC 2 cut(s) 232, 807
BsmI GAATGC 1 cut(s) 426
BsnI GGCC 3 cut(s) 5, 276, 682
Bso31I GGTCTC 1 cut(s) 506
Bsp119I TTCGAA 1 cut(s) 430
Bsp143I GATC 1 cut(s) 379
Bsp19I CCATGG 1 cut(s) 897
BspACI CCGC 4 cut(s) 252, 402, 683, 727
BspANI GGCC 3 cut(s) 5, 276, 682
BspCNI CTCAG 1 cut(s) 63
BspLI GGNNCC 1 cut(s) 807
BspMAI CTGCAG 2 cut(s) 470, 862
BspT104I TTCGAA 1 cut(s) 430
BspT107I GGYRCC 1 cut(s) 805
BspTNI GGTCTC 1 cut(s) 506
BsrDI GCAATG 2 cut(s) 937, 951
BsrFI RCCGGY 1 cut(s) 808
BsrI ACTGG 1 cut(s) 292
BssAI RCCGGY 1 cut(s) 808
BssECI CCNNGG 3 cut(s) 271, 516, 897
BssMI GATC 1 cut(s) 379
BssNI GRCGYC 2 cut(s) 806, 812
BssSI CACGAG 1 cut(s) 509
BssT1I CCWWGG 1 cut(s) 897
Bst2BI CACGAG 1 cut(s) 509
Bst2UI CCWGG 1 cut(s) 517
Bst4CI ACNGT 2 cut(s) 81, 798
Bst6I CTCTTC 3 cut(s) 653, 897, 957
BstACI GRCGYC 2 cut(s) 806, 812
BstBAI YACGTR 1 cut(s) 667
BstBI TTCGAA 1 cut(s) 430
BstC8I GCNNGC 3 cut(s) 56, 810, 912
BstDEI CTNAG 1 cut(s) 50
BstDSI CCRYGG 2 cut(s) 271, 897
BstF5I GGATG 2 cut(s) 441, 795
BstH2I RGCGCY 1 cut(s) 809
BstHHI GCGC 1 cut(s) 808
BstKTI GATC 1 cut(s) 382
BstMAI GTCTC 2 cut(s) 319, 506
BstMBI GATC 1 cut(s) 379
BstMWI GCNNNNNNNGC 5 cut(s) 471, 724, 860, 923, 932
BstNI CCWGG 1 cut(s) 517
BstNSI RCATGY 1 cut(s) 763
BstSCI CCNGG 1 cut(s) 515
BstSFI CTRYAG 2 cut(s) 466, 858
BstV1I GCAGC 7 cut(s) 452, 480, 483, 742, 872, 901, 913
BsuRI GGCC 3 cut(s) 5, 276, 682
BtgI CCRYGG 2 cut(s) 271, 897
BtrI CACGTC 2 cut(s) 180, 494
BtsCI GGATG 2 cut(s) 441, 795
Cac8I GCNNGC 3 cut(s) 56, 810, 912
CfoI GCGC 1 cut(s) 808
Cfr10I RCCGGY 1 cut(s) 808
CseI GACGC 1 cut(s) 801
Csp6I GTAC 1 cut(s) 664
CviAII CATG 5 cut(s) 59, 134, 760, 773, 898
CviQI GTAC 1 cut(s) 664
DdeI CTNAG 1 cut(s) 50
DinI GGCGCC 1 cut(s) 807
DpnI GATC 1 cut(s) 381
DpnII GATC 1 cut(s) 379
EaeI YGGCCR 2 cut(s) 3, 274
Eam1104I CTCTTC 3 cut(s) 653, 897, 957
EarI CTCTTC 3 cut(s) 653, 897, 957
Eco130I CCWWGG 1 cut(s) 897
Eco31I GGTCTC 1 cut(s) 506
Eco57I CTGAAG 1 cut(s) 677
EcoRI GAATTC 1 cut(s) 137
EcoRII CCWGG 1 cut(s) 515
EcoT14I CCWWGG 1 cut(s) 897
EgeI GGCGCC 1 cut(s) 807
EheI GGCGCC 1 cut(s) 807
ErhI CCWWGG 1 cut(s) 897
Esp3I CGTCTC 1 cut(s) 319
FaeI CATG 5 cut(s) 62, 137, 763, 776, 901
FaiI YATR 6 cut(s) 60, 135, 653, 761, 774, 899
FaqI GGGAC 2 cut(s) 232, 807
FatI CATG 5 cut(s) 58, 133, 759, 772, 897
FauI CCCGC 1 cut(s) 259
Fnu4HI GCNGC 9 cut(s) 466, 469, 472, 683, 728, 731, 861, 915, 927
FokI GGATG 2 cut(s) 428, 802
Fsp4HI GCNGC 9 cut(s) 466, 469, 472, 683, 728, 731, 861, 915, 927
FspBI CTAG 2 cut(s) 41, 561
GlaI GCGC 1 cut(s) 807
GluI GCNGC 9 cut(s) 466, 469, 472, 683, 728, 731, 861, 915, 927
GsuI CTGGAG 1 cut(s) 275
HaeII RGCGCY 1 cut(s) 809
HaeIII GGCC 3 cut(s) 5, 276, 682
HapII CCGG 2 cut(s) 724, 809
HgaI GACGC 1 cut(s) 801
HhaI GCGC 1 cut(s) 808
Hin1I GRCGYC 2 cut(s) 806, 812
Hin1II CATG 5 cut(s) 62, 137, 763, 776, 901
Hin6I GCGC 1 cut(s) 806
HinP1I GCGC 1 cut(s) 806
HinfI GANTC 2 cut(s) 486, 646
HpaII CCGG 2 cut(s) 724, 809
HphI GGTGA 4 cut(s) 96, 185, 343, 688
Hpy188I TCNGA 1 cut(s) 831
Hpy188III TCNNGA 1 cut(s) 41
Hpy99I CGWCG 1 cut(s) 835
HpyAV CCTTC 6 cut(s) 120, 154, 237, 622, 706, 780
HpyCH4III ACNGT 2 cut(s) 81, 798
HpyCH4IV ACGT 3 cut(s) 179, 493, 666
HpyCH4V TGCA 8 cut(s) 426, 468, 854, 860, 910, 917, 929, 942
HpyF10VI GCNNNNNNNGC 5 cut(s) 471, 724, 860, 923, 932
HpyF3I CTNAG 1 cut(s) 50
HpySE526I ACGT 3 cut(s) 179, 493, 666
Hsp92I GRCGYC 2 cut(s) 806, 812
Hsp92II CATG 5 cut(s) 62, 137, 763, 776, 901
HspAI GCGC 1 cut(s) 806
KasI GGCGCC 1 cut(s) 805
KroI GCCGGC 1 cut(s) 808
KroNI GCCGGC 1 cut(s) 810
Kzo9I GATC 1 cut(s) 379
LmnI GCTCC 1 cut(s) 329
Lsp1109I GCAGC 7 cut(s) 452, 480, 483, 742, 872, 901, 913
LweI GCATC 2 cut(s) 483, 853
MaeI CTAG 2 cut(s) 41, 561
MaeII ACGT 3 cut(s) 179, 493, 666
MaeIII GTNAC 3 cut(s) 81, 647, 887
MalI GATC 1 cut(s) 381
MboI GATC 1 cut(s) 379
MfeI CAATTG 1 cut(s) 930
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 5 cut(s) 33, 137, 751, 918, 930
MluNI TGGCCA 1 cut(s) 5
Mly113I GGCGCC 1 cut(s) 806
MlyI GAGTC 1 cut(s) 655
MmeI TCCRAC 1 cut(s) 854
Mox20I TGGCCA 1 cut(s) 5
MreI CGCCGGCG 1 cut(s) 808
MroNI GCCGGC 1 cut(s) 808
MscI TGGCCA 1 cut(s) 5
MseI TTAA 2 cut(s) 74, 764
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 3 cut(s) 16, 465, 685
MspI CCGG 2 cut(s) 724, 809
MspR9I CCNGG 1 cut(s) 517
MunI CAATTG 1 cut(s) 930
Mva1269I GAATGC 1 cut(s) 426
MvaI CCWGG 1 cut(s) 517
MwoI GCNNNNNNNGC 5 cut(s) 471, 724, 860, 923, 932
NaeI GCCGGC 1 cut(s) 810
NarI GGCGCC 1 cut(s) 806
NcoI CCATGG 1 cut(s) 897
NdeII GATC 1 cut(s) 379
NgoMIV GCCGGC 1 cut(s) 808
NlaIII CATG 5 cut(s) 62, 137, 763, 776, 901
NlaIV GGNNCC 1 cut(s) 807
NmuCI GTSAC 2 cut(s) 647, 887
NspI RCATGY 1 cut(s) 763
NspV TTCGAA 1 cut(s) 430
PcsI WCGNNNNNNNCGW 1 cut(s) 809
PctI GAATGC 1 cut(s) 426
PdiI GCCGGC 1 cut(s) 810
PfeI GAWTC 1 cut(s) 486
PflMI CCANNNNNTGG 1 cut(s) 16
PkrI GCNGC 9 cut(s) 467, 470, 473, 684, 729, 732, 862, 916, 928
PleI GAGTC 1 cut(s) 654
PluTI GGCGCC 1 cut(s) 809
PpsI GAGTC 1 cut(s) 654
Ppu21I YACGTR 1 cut(s) 667
Psp6I CCWGG 1 cut(s) 515
PspGI CCWGG 1 cut(s) 515
PspN4I GGNNCC 1 cut(s) 807
PstI CTGCAG 2 cut(s) 470, 862
PvuII CAGCTG 2 cut(s) 16, 465
RsaI GTAC 1 cut(s) 665
RsaNI GTAC 1 cut(s) 664
SaqAI TTAA 2 cut(s) 74, 764
SatI GCNGC 9 cut(s) 466, 469, 472, 683, 728, 731, 861, 915, 927
Sau3AI GATC 1 cut(s) 379
SchI GAGTC 1 cut(s) 655
ScrFI CCNGG 1 cut(s) 517
SfaNI GCATC 2 cut(s) 483, 853
SfcI CTRYAG 2 cut(s) 466, 858
SfoI GGCGCC 1 cut(s) 807
SfuI TTCGAA 1 cut(s) 430
SgrAI CRCCGGYG 1 cut(s) 808
Sse9I AATT 5 cut(s) 33, 137, 751, 918, 930
SsiI CCGC 4 cut(s) 252, 402, 683, 727
SspDI GGCGCC 1 cut(s) 805
SspI AATATT 2 cut(s) 264, 575
SspMI CTAG 2 cut(s) 41, 561
StyD4I CCNGG 1 cut(s) 515
StyI CCWWGG 1 cut(s) 897
TaaI ACNGT 2 cut(s) 81, 798
TaiI ACGT 3 cut(s) 182, 496, 669
TaqI TCGA 4 cut(s) 342, 430, 477, 545
TasI AATT 5 cut(s) 33, 137, 751, 918, 930
TauI GCSGC 2 cut(s) 685, 730
TfiI GAWTC 1 cut(s) 486
Tru1I TTAA 2 cut(s) 74, 764
Tru9I TTAA 2 cut(s) 74, 764
TseFI GTSAC 2 cut(s) 647, 887
TseI GCWGC 7 cut(s) 465, 468, 471, 730, 860, 914, 926
Tsp45I GTSAC 2 cut(s) 647, 887
TspDTI ATGAA 5 cut(s) 75, 102, 150, 273, 629
Van91I CCANNNNNTGG 1 cut(s) 16
XapI RAATTY 2 cut(s) 137, 751
XbaI TCTAGA 1 cut(s) 40
XceI RCATGY 1 cut(s) 763
XcmI CCANNNNNNNNNTGG 1 cut(s) 13
XspI CTAG 2 cut(s) 41, 561
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.