Rw6G041010

BRI1 kinase inhibitor 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0012335
Physical Location & Seq
Forward (+)
64721646 .. 64723323
1678 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G041010.1

Sequence Viewer

Length: 945 bp
ATGGACACCCATCTGCTTGAAAAGACCAGAGAAAAAGCTGCAGGCAACAACATGCTTCAAGAAAAGCCACAGTCACCGGTTACAGCCTCACCACACTCAGCTTCCTCTTCTCCTTCTCATGAATTCTCCTTCACAGTCTCTCTTCATCACTCTTCTTCCACATCAATCCCTGATCATAGTAAGAACAACAACAAAGCCAAAACTCCGCCTTCTTTCGCTATTGACTTGTCTCCTGCAGATGACATTTTCTTCCATGGCCATTTGCTTCCTTTGCAGTTCCTCTCTCACCTTCCTATCTCTCCTCGCTCCTCTACTAACTCCTTAGATAGCTTCACTCTTCCCATTAGAGACTTAGTAGACGATCAAGAACCAACCAAGGAAAGCAACAACTGTAGCACCAGCATTGGGAGCAACAACATCATCAACAAGAAGAAGAGCAACAGCAGCAGCAATATCCAAGAGAGCAAGAGCCGCAATGGCGAGACGAGGGGGAGAACCAAGTCCAAGTCCTTTTCGATTTTCGGGTTACCAAAAAGGCGAAAAGATTGCGAAGAGAGGGAGGACAAGGAGGGGAAGCACAAGAGGAAGATGAGGTTTGATGTGAGTAATATACTAAAGAGGTATGTGAGGATGGTCAGGCCCTTATTGTTCTTTAGAGGAAGAAAAGAGAATGTCCATTTTAGTAGGCAGCCTTACTCGTTTTCGGGTAATTTGAGCCCCAGAAATAAGCATCACTTGAGAGGAAGGAGAGGAGAGTTCTCAGCACCAGCATCAATGAGGACATCTCCAACAAATAGTGGGCTTCTCCTAGCAACTTCAGGAGCAACTACTCCTACTAATACCAGTGATAGTACCATGGAAGAGTTGCAAGCAGCAATTCAAGCCGCAATTGCTCATTGCAAGAATTCCATTGCCACAGAAGAGAAGATTAAATGCGTAGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
No domains found.

Protein Analysis

314

Amino Acids

34.97

Weight (kDa)

9.8

Isoelectric Point (pI)

56.38

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012335)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G42750
fragaria_vesca FvH4_2g34560
malus_domestica MD08G1102600.v1.1 MD15G1084100.v1.1
prunus_persica Prupe.1G437500_v2.0.a1
pyrus_communis pycom08g08410 pycom15g07930
rosa_chinensis RchiOBHm_Chr6g0307601 RchiOBHm_Chr6g0307761
rosa_laevigata RLG00000010702
rosa_multiflora Rmu_co8485591.1_g000001 Rmu_sc0000031.1_g000029
rosa_roxburghii Rroxscaffold_7G00160910
rosa_rugosa Rorug06G0360600
rosa_samantha Rh6AG469700 Rh6BG480200 Rh6CG485900 Rh6DG472400
rosa_wichuraiana Rw6G041010

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 357
AciI CCGC 3 cut(s) 206, 472, 885
AcoI YGGCCR 1 cut(s) 256
AcsI RAATTY 2 cut(s) 122, 904
AcuI CTGAAG 1 cut(s) 801
AfaI GTAC 1 cut(s) 853
AfiI CCNNNNNNNGG 1 cut(s) 405
AgeI ACCGGT 1 cut(s) 76
AgsI TTSAA 3 cut(s) 20, 59, 881
AluBI AGCT 3 cut(s) 38, 101, 330
AluI AGCT 3 cut(s) 38, 101, 330
Alw26I GTCTC 4 cut(s) 142, 234, 342, 476
AoxI GGCC 2 cut(s) 256, 638
ApeKI GCWGC 5 cut(s) 38, 444, 447, 688, 872
ApoI RAATTY 2 cut(s) 122, 904
AsiGI ACCGGT 1 cut(s) 76
AspS9I GGNCC 1 cut(s) 639
AsuHPI GGTGA 3 cut(s) 66, 81, 278
BalI TGGCCA 1 cut(s) 258
BanII GRGCYC 1 cut(s) 719
BbvI GCAGC 5 cut(s) 25, 456, 459, 700, 884
BccI CCATC 2 cut(s) 18, 625
BcgI CGANNNNNNTGC 2 cut(s) 528, 562
BclI TGATCA 1 cut(s) 172
BcoDI GTCTC 4 cut(s) 142, 234, 342, 476
BfaI CTAG 1 cut(s) 809
BfmI CTRYAG 3 cut(s) 39, 234, 391
BglI GCCNNNNNGGC 1 cut(s) 477
BisI GCNGC 7 cut(s) 39, 445, 448, 472, 689, 873, 885
BlsI GCNGC 7 cut(s) 40, 446, 449, 473, 690, 874, 886
BmgT120I GGNCC 1 cut(s) 639
BmsI GCATC 2 cut(s) 739, 779
BplI GAGNNNNNCTC 2 cut(s) 769, 801
BpuEI CTTGAG 1 cut(s) 757
BsaJI CCNNGG 3 cut(s) 253, 375, 855
BsaWI WCCGGW 1 cut(s) 76
Bsc4I CCNNNNNNNGG 1 cut(s) 405
Bse118I RCCGGY 1 cut(s) 76
Bse1I ACTGG 1 cut(s) 843
Bse3DI GCAATG 3 cut(s) 481, 895, 909
BseDI CCNNGG 3 cut(s) 253, 375, 855
BseGI GGATG 1 cut(s) 636
BseLI CCNNNNNNNGG 1 cut(s) 405
BseMI GCAATG 3 cut(s) 481, 895, 909
BseMII CTCAG 2 cut(s) 111, 774
BseNI ACTGG 1 cut(s) 843
BseRI GAGGAG 3 cut(s) 291, 298, 765
BseXI GCAGC 5 cut(s) 25, 456, 459, 700, 884
BshFI GGCC 2 cut(s) 258, 640
BshTI ACCGGT 1 cut(s) 76
BsiSI CCGG 1 cut(s) 77
BslI CCNNNNNNNGG 1 cut(s) 405
BsmAI GTCTC 4 cut(s) 142, 234, 342, 476
BsmBI CGTCTC 1 cut(s) 476
BsnI GGCC 2 cut(s) 258, 640
Bsp1286I GDGCHC 1 cut(s) 719
Bsp143I GATC 2 cut(s) 172, 361
Bsp19I CCATGG 2 cut(s) 253, 855
BspACI CCGC 3 cut(s) 206, 472, 885
BspANI GGCC 2 cut(s) 258, 640
BspCNI CTCAG 2 cut(s) 110, 773
BspHI TCATGA 1 cut(s) 118
BspMAI CTGCAG 2 cut(s) 43, 238
BspQI GCTCTTC 1 cut(s) 428
BsrDI GCAATG 3 cut(s) 481, 895, 909
BsrFI RCCGGY 1 cut(s) 76
BsrI ACTGG 1 cut(s) 843
BssAI RCCGGY 1 cut(s) 76
BssECI CCNNGG 3 cut(s) 253, 375, 855
BssMI GATC 2 cut(s) 172, 361
BssT1I CCWWGG 3 cut(s) 253, 375, 855
Bst4CI ACNGT 3 cut(s) 72, 136, 392
Bst6I CTCTTC 8 cut(s) 112, 147, 157, 342, 428, 546, 855, 915
BstC8I GCNNGC 2 cut(s) 43, 870
BstDEI CTNAG 4 cut(s) 97, 322, 352, 760
BstDSI CCRYGG 2 cut(s) 253, 855
BstEII GGTNACC 1 cut(s) 525
BstF5I GGATG 1 cut(s) 636
BstKTI GATC 2 cut(s) 175, 364
BstMAI GTCTC 4 cut(s) 142, 234, 342, 476
BstMBI GATC 2 cut(s) 172, 361
BstMWI GCNNNNNNNGC 7 cut(s) 271, 408, 444, 471, 477, 881, 890
BstNSI RCATGY 1 cut(s) 55
BstPI GGTNACC 1 cut(s) 525
BstSFI CTRYAG 3 cut(s) 39, 234, 391
BstV1I GCAGC 5 cut(s) 25, 456, 459, 700, 884
BsuRI GGCC 2 cut(s) 258, 640
BtgI CCRYGG 2 cut(s) 253, 855
BtsCI GGATG 1 cut(s) 636
BtsIMutI CAGTG 1 cut(s) 850
Cac8I GCNNGC 2 cut(s) 43, 870
CciI TCATGA 1 cut(s) 118
Cfr10I RCCGGY 1 cut(s) 76
Cfr13I GGNCC 1 cut(s) 639
Csp6I GTAC 1 cut(s) 852
CspAI ACCGGT 1 cut(s) 76
CviAII CATG 4 cut(s) 52, 119, 254, 856
CviQI GTAC 1 cut(s) 852
DdeI CTNAG 4 cut(s) 97, 322, 352, 760
DpnI GATC 2 cut(s) 174, 363
DpnII GATC 2 cut(s) 172, 361
EaeI YGGCCR 1 cut(s) 256
Eam1104I CTCTTC 8 cut(s) 112, 147, 157, 342, 428, 546, 855, 915
EarI CTCTTC 8 cut(s) 112, 147, 157, 342, 428, 546, 855, 915
EciI GGCGGA 1 cut(s) 195
Eco130I CCWWGG 3 cut(s) 253, 375, 855
Eco24I GRGCYC 1 cut(s) 719
Eco57I CTGAAG 1 cut(s) 801
Eco91I GGTNACC 1 cut(s) 525
EcoO109I RGGNCCY 1 cut(s) 639
EcoO65I GGTNACC 1 cut(s) 525
EcoRI GAATTC 2 cut(s) 122, 904
EcoT14I CCWWGG 3 cut(s) 253, 375, 855
EcoT38I GRGCYC 1 cut(s) 719
ErhI CCWWGG 3 cut(s) 253, 375, 855
Esp3I CGTCTC 1 cut(s) 476
FaeI CATG 4 cut(s) 55, 122, 257, 859
FaiI YATR 7 cut(s) 53, 120, 177, 255, 611, 624, 857
FalI AAGNNNNNCTT 2 cut(s) 719, 751
FatI CATG 4 cut(s) 51, 118, 253, 855
FbaI TGATCA 1 cut(s) 172
FblI GTMKAC 1 cut(s) 357
Fnu4HI GCNGC 7 cut(s) 39, 445, 448, 472, 689, 873, 885
FokI GGATG 1 cut(s) 643
FriOI GRGCYC 1 cut(s) 719
Fsp4HI GCNGC 7 cut(s) 39, 445, 448, 472, 689, 873, 885
FspBI CTAG 1 cut(s) 809
GluI GCNGC 7 cut(s) 39, 445, 448, 472, 689, 873, 885
HaeIII GGCC 2 cut(s) 258, 640
HapII CCGG 1 cut(s) 77
Hin1II CATG 4 cut(s) 55, 122, 257, 859
HpaII CCGG 1 cut(s) 77
HphI GGTGA 3 cut(s) 66, 81, 278
Hpy166II GTNNAC 1 cut(s) 358
Hpy188III TCNNGA 4 cut(s) 59, 119, 365, 819
Hpy8I GTNNAC 1 cut(s) 358
HpyAV CCTTC 5 cut(s) 123, 139, 219, 299, 738
HpyCH4III ACNGT 3 cut(s) 72, 136, 392
HpyCH4V TGCA 5 cut(s) 41, 236, 274, 868, 900
HpyF10VI GCNNNNNNNGC 7 cut(s) 271, 408, 444, 471, 477, 881, 890
HpyF3I CTNAG 4 cut(s) 97, 322, 352, 760
Hsp92II CATG 4 cut(s) 55, 122, 257, 859
Ksp22I TGATCA 1 cut(s) 172
Kzo9I GATC 2 cut(s) 172, 361
LguI GCTCTTC 1 cut(s) 428
LmnI GCTCC 3 cut(s) 311, 408, 821
Lsp1109I GCAGC 5 cut(s) 25, 456, 459, 700, 884
LweI GCATC 2 cut(s) 739, 779
MaeI CTAG 1 cut(s) 809
MaeIII GTNAC 3 cut(s) 72, 79, 525
MalI GATC 2 cut(s) 174, 363
MboI GATC 2 cut(s) 172, 361
MfeI CAATTG 1 cut(s) 888
MhlI GDGCHC 1 cut(s) 719
MlsI TGGCCA 1 cut(s) 258
MluCI AATT 5 cut(s) 122, 709, 876, 888, 904
MluNI TGGCCA 1 cut(s) 258
MmeI TCCRAC 1 cut(s) 812
Mox20I TGGCCA 1 cut(s) 258
MscI TGGCCA 1 cut(s) 258
MseI TTAA 1 cut(s) 930
Msp20I TGGCCA 1 cut(s) 258
MspI CCGG 1 cut(s) 77
MunI CAATTG 1 cut(s) 888
MwoI GCNNNNNNNGC 7 cut(s) 271, 408, 444, 471, 477, 881, 890
NcoI CCATGG 2 cut(s) 253, 855
NdeII GATC 2 cut(s) 172, 361
NlaIII CATG 4 cut(s) 55, 122, 257, 859
NmuCI GTSAC 1 cut(s) 72
NspI RCATGY 1 cut(s) 55
PagI TCATGA 1 cut(s) 118
PciSI GCTCTTC 1 cut(s) 428
PinAI ACCGGT 1 cut(s) 76
PkrI GCNGC 7 cut(s) 40, 446, 449, 473, 690, 874, 886
PspEI GGTNACC 1 cut(s) 525
PspPI GGNCC 1 cut(s) 639
PstI CTGCAG 2 cut(s) 43, 238
RsaI GTAC 1 cut(s) 853
RsaNI GTAC 1 cut(s) 852
SapI GCTCTTC 1 cut(s) 428
SaqAI TTAA 1 cut(s) 930
SatI GCNGC 7 cut(s) 39, 445, 448, 472, 689, 873, 885
Sau3AI GATC 2 cut(s) 172, 361
Sau96I GGNCC 1 cut(s) 639
SduI GDGCHC 1 cut(s) 719
SetI ASST 6 cut(s) 40, 103, 291, 332, 596, 623
SfaNI GCATC 2 cut(s) 739, 779
SfcI CTRYAG 3 cut(s) 39, 234, 391
SmlI CTYRAG 1 cut(s) 736
SmoI CTYRAG 1 cut(s) 736
Sse9I AATT 5 cut(s) 122, 709, 876, 888, 904
SsiI CCGC 3 cut(s) 206, 472, 885
SspMI CTAG 1 cut(s) 809
StyI CCWWGG 3 cut(s) 253, 375, 855
TaaI ACNGT 3 cut(s) 72, 136, 392
TaqI TCGA 1 cut(s) 515
TasI AATT 5 cut(s) 122, 709, 876, 888, 904
TauI GCSGC 2 cut(s) 474, 887
Tru1I TTAA 1 cut(s) 930
Tru9I TTAA 1 cut(s) 930
TscAI CASTG 1 cut(s) 850
TseFI GTSAC 1 cut(s) 72
TseI GCWGC 5 cut(s) 38, 444, 447, 688, 872
Tsp45I GTSAC 1 cut(s) 72
TspDTI ATGAA 2 cut(s) 134, 135
TspRI CASTG 1 cut(s) 850
XapI RAATTY 2 cut(s) 122, 904
XceI RCATGY 1 cut(s) 55
XmiI GTMKAC 1 cut(s) 357
XspI CTAG 1 cut(s) 809
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.