pycom08g12880

ACT domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0011257
Physical Location & Seq
Forward (+)
11453898 .. 11456310
2413 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1575 bp
ATGTGCAGCATGAAGAAAGTTTGTTGGCCTTACTTCGATCCTGACTTTGATAATCTCCCAGAGAGGATATATGGCCCTGCTTGTCAAGTAAGCATCGACAACGAAGGGATGGAAGATTGCACAGTAGTAAAGGTGGATAGCGTGAACAAGCAAGGGCTTCTCCTGGAAGTGGTGCAAGTTTTGACAGACATGAACCTCACCATCACTAAAAGTTACATTTCTTCTGATGCTGGATGGTTCATGGATGGTATGAACATATCAAACTTCATCACCCATCAGCCCCGTAAATTAATTTCTCAATATATATTAGAAACCCCCTGTATAATTTTTTTTAATTTGTTAATCACTATCTTAGTTATGATCACTAATTTTGTGCACGCTTTTGCAGTTTTTCATGTGAAAGATGAACATGGCAACAAAGTTACAGACCGGAATGTCCTTAACTATATTCAACAGGCCATAGTTACAGCCACAGGCCCCCCAGACTTGGCCAAGTCCTATGCCAACAACCACCCTATTTTATTCGAACCCGAAAACCCTATTGAACACACCAGTGAAAATCCTAGCGAGCACACTACCATTGAAATGACCGGTACAGACCGGCCCGGTCTGTTTTCGGAGATCTCCGCTGCCCTGGCGGATCTCCAATGCAACATAGTAGAAGCGCATGCATGGAGCCACAATGCCCGCCTGGCATGTGTTGCTCAAATATCTGATAAATCAACCGACACCCCAATCGACGACCCCCGACGCATTGCCACCATAGAAGACCACCTCATCACCGTCCTCCGTGCCACCACAGCCCTCAGCCCGTTTGGGAAGGAACCCAATTGTCAACAAGAAGTGAAGACCTCTGGGCTTATGGGAGGAGGAGATAGTAATCATAATATTCACCAAGGCACCATGAGTACTAATGTGGACCGTAGGTTGCACCAGCTCATGCTTTCTGTGAGGGACTTTGATGGGCCTCGCTTGGGGCCTGCAAGCTCCCCAAGGACGCCCTTGGGGTTGGATAGTGAGGAGGGGAGTCGGAAGCCAGTGGTTTGGATTGAGAGTTGTAAGGAAAAAGGGTATTCAATGGTGACTATCGAGTGCAAGGATCGGCCGAGGCTCATGTTTGATACTGTGTGCACCCTCACTGACATGCAATATGTAATTTTCCATGCTTCTGCTAGTGCCCAAGAAGGTTATTCCTTTCAGGGTGTTCGGGTAGAACTATGCGCAGACAATAGGATAGGGTTGCTCTCGGATATAACTAGGGTTCTACGGGAGAATGGACTTGCGGTTGTTCGAGCAGATGTAGCAACGCAAGGAGAAAAGGCCGTAAACGCCTTCTACGTCAGGGATATTTCAGGCAAAGAAGTAGTAGACATGGATTTGATCGAGTCGATGAAGAGAGAATTAGTGGGTGAAAATCCAATAGAATTTCAAGTCAAGAATGACACATGGATGAGACCGAACTCACTTGAAAAAGGGTCGTCCCCTCTCTCTAATTTTGGAGACATGCTGAAATCTCAGATAGAGCGATTTTCGTACAAGTTTGTCAAGACCAAGCAGGAGGAATTGAACCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ACT ACT_6

Protein Analysis

525

Amino Acids

58.66

Weight (kDa)

5.22

Isoelectric Point (pI)

40.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ACT PF01842 192 - 227 3.4e-06 ACT domain
ACT_6 PF13740 194 - 238 1.1e-06 ACT domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011257)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G25320 AT5G25320 AT5G25320
fragaria_vesca FvH4_2g28840 FvH4_2g28840 FvH4_2g28840 FvH4_2g28840 FvH4_2g28840
malus_domestica MD08G1156600.v1.1 MD15G1127200.v1.1
prunus_persica Prupe.1G482900_v2.0.a1
pyrus_communis pycom08g12880
rosa_chinensis RchiOBHm_Chr6g0297601
rosa_laevigata RLG00000011578
rosa_rugosa Rorug06G0274200
rosa_samantha Rh6AG385000 Rh6BG393100 Rh6CG398900 Rh6DG385500
rosa_wichuraiana Rw6G033540

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 434
Acc16I TGCGCA 1 cut(s) 1222
AccB1I GGYRCC 1 cut(s) 899
AccI GTMKAC 1 cut(s) 1368
AciI CCGC 4 cut(s) 627, 638, 688, 1283
AclWI GGATC 3 cut(s) 32, 648, 1107
AcoI YGGCCR 2 cut(s) 489, 1103
AcsI RAATTY 1 cut(s) 1424
AcyI GRCGYC 1 cut(s) 998
AfaI GTAC 3 cut(s) 595, 910, 1535
AfiI CCNNNNNNNGG 3 cut(s) 169, 487, 974
AgeI ACCGGT 1 cut(s) 590
AgsI TTSAA 7 cut(s) 452, 545, 584, 1077, 1430, 1469, 1567
AjnI CCWGG 3 cut(s) 162, 633, 690
AleI CACNNNNGTG 1 cut(s) 552
AluBI AGCT 2 cut(s) 937, 987
AluI AGCT 2 cut(s) 937, 987
Alw21I GWGCWC 3 cut(s) 378, 573, 1133
Alw26I GTCTC 2 cut(s) 1447, 1494
Alw44I GTGCAC 2 cut(s) 374, 1129
AlwI GGATC 3 cut(s) 32, 648, 1107
ApaLI GTGCAC 2 cut(s) 374, 1129
ApeKI GCWGC 2 cut(s) 6, 629
ApoI RAATTY 1 cut(s) 1424
AseI ATTAAT 1 cut(s) 290
AsiGI ACCGGT 1 cut(s) 590
AspLEI GCGC 2 cut(s) 667, 1223
AspS9I GGNCC 6 cut(s) 74, 476, 603, 919, 965, 977
AsuC2I CCSGG 1 cut(s) 606
AsuHPI GGTGA 6 cut(s) 190, 262, 772, 884, 1093, 1421
AsuII TTCGAA 1 cut(s) 525
AvaII GGWCC 1 cut(s) 919
BaeGI GKGCMC 3 cut(s) 378, 1133, 1180
BalI TGGCCA 1 cut(s) 491
BanI GGYRCC 1 cut(s) 899
BbsI GAAGAC 2 cut(s) 774, 854
Bbv12I GWGCWC 3 cut(s) 378, 573, 1133
BbvCI CCTCAGC 1 cut(s) 806
BbvI GCAGC 2 cut(s) 18, 616
BccI CCATC 6 cut(s) 103, 209, 228, 239, 282, 956
BceAI ACGGC 1 cut(s) 1307
BciT130I CCWGG 3 cut(s) 164, 635, 692
BclI TGATCA 1 cut(s) 360
BcnI CCSGG 1 cut(s) 606
BcoDI GTCTC 2 cut(s) 1447, 1494
BfaI CTAG 3 cut(s) 564, 1173, 1257
BglI GCCNNNNNGGC 1 cut(s) 692
BglII AGATCT 1 cut(s) 621
BisI GCNGC 2 cut(s) 7, 630
BlsI GCNGC 2 cut(s) 8, 631
BmcAI AGTACT 1 cut(s) 910
Bme1390I CCNGG 4 cut(s) 164, 606, 635, 692
Bme18I GGWCC 1 cut(s) 919
BmgT120I GGNCC 6 cut(s) 74, 476, 603, 919, 965, 977
BmiI GGNNCC 5 cut(s) 478, 677, 825, 901, 978
BmrFI CCNGG 4 cut(s) 164, 606, 635, 692
BmsI GCATC 2 cut(s) 102, 217
BpiI GAAGAC 2 cut(s) 774, 854
Bpu10I CCTNAGC 1 cut(s) 806
Bpu14I TTCGAA 1 cut(s) 525
BpuMI CCSGG 1 cut(s) 606
BsaBI GATNNNNATC 1 cut(s) 879
BsaHI GRCGYC 1 cut(s) 998
BsaI GGTCTC 1 cut(s) 1447
BsaJI CCNNGG 5 cut(s) 633, 895, 992, 1002, 1106
BsaWI WCCGGW 2 cut(s) 429, 590
Bsc4I CCNNNNNNNGG 3 cut(s) 169, 487, 974
Bse118I RCCGGY 2 cut(s) 590, 600
Bse1I ACTGG 2 cut(s) 552, 1037
Bse3DI GCAATG 1 cut(s) 753
Bse8I GATNNNNATC 1 cut(s) 879
BseBI CCWGG 3 cut(s) 164, 635, 692
BseDI CCNNGG 5 cut(s) 633, 895, 992, 1002, 1106
BseGI GGATG 4 cut(s) 114, 239, 250, 1455
BseJI GATNNNNATC 1 cut(s) 879
BseLI CCNNNNNNNGG 3 cut(s) 169, 487, 974
BseMI GCAATG 1 cut(s) 753
BseMII CTCAG 2 cut(s) 820, 1529
BseNI ACTGG 2 cut(s) 552, 1037
BseRI GAGGAG 3 cut(s) 882, 885, 1034
BseSI GKGCMC 3 cut(s) 378, 1133, 1180
BseX3I CGGCCG 1 cut(s) 1103
BseXI GCAGC 2 cut(s) 18, 616
BsgI GTGCAG 1 cut(s) 25
Bsh1285I CGRYCG 1 cut(s) 1106
BshNI GGYRCC 1 cut(s) 899
BshTI ACCGGT 1 cut(s) 590
BsiEI CGRYCG 1 cut(s) 1106
BsiHKAI GWGCWC 3 cut(s) 378, 573, 1133
BsiSI CCGG 4 cut(s) 430, 591, 601, 606
BslFI GGGAC 2 cut(s) 968, 1465
BslI CCNNNNNNNGG 3 cut(s) 169, 487, 974
BsmAI GTCTC 2 cut(s) 1447, 1494
BsmFI GGGAC 2 cut(s) 968, 1465
Bso31I GGTCTC 1 cut(s) 1447
Bsp119I TTCGAA 1 cut(s) 525
Bsp1286I GDGCHC 4 cut(s) 378, 573, 1133, 1180
Bsp143I GATC 6 cut(s) 37, 360, 621, 640, 1099, 1380
BspACI CCGC 4 cut(s) 627, 638, 688, 1283
BspCNI CTCAG 2 cut(s) 819, 1528
BspLI GGNNCC 5 cut(s) 478, 677, 825, 901, 978
BspPI GGATC 3 cut(s) 32, 648, 1107
BspT104I TTCGAA 1 cut(s) 525
BspT107I GGYRCC 1 cut(s) 899
BspTNI GGTCTC 1 cut(s) 1447
BsrDI GCAATG 1 cut(s) 753
BsrFI RCCGGY 2 cut(s) 590, 600
BsrI ACTGG 2 cut(s) 552, 1037
BssAI RCCGGY 2 cut(s) 590, 600
BssECI CCNNGG 5 cut(s) 633, 895, 992, 1002, 1106
BssMI GATC 6 cut(s) 37, 360, 621, 640, 1099, 1380
BssNI GRCGYC 1 cut(s) 998
BssT1I CCWWGG 3 cut(s) 895, 992, 1002
Bst2UI CCWGG 3 cut(s) 164, 635, 692
Bst4CI ACNGT 4 cut(s) 124, 784, 923, 1126
Bst6I CTCTTC 1 cut(s) 1388
BstACI GRCGYC 1 cut(s) 998
BstAPI GCANNNNNTGC 1 cut(s) 701
BstBI TTCGAA 1 cut(s) 525
BstC8I GCNNGC 6 cut(s) 378, 569, 669, 688, 981, 985
BstDEI CTNAG 3 cut(s) 352, 806, 1515
BstF5I GGATG 4 cut(s) 114, 239, 250, 1455
BstHHI GCGC 2 cut(s) 667, 1223
BstKTI GATC 6 cut(s) 40, 363, 624, 643, 1102, 1383
BstMAI GTCTC 2 cut(s) 1447, 1494
BstMBI GATC 6 cut(s) 37, 360, 621, 640, 1099, 1380
BstMCI CGRYCG 1 cut(s) 1106
BstMWI GCNNNNNNNGC 6 cut(s) 635, 692, 701, 800, 1301, 1328
BstNI CCWGG 3 cut(s) 164, 635, 692
BstNSI RCATGY 4 cut(s) 671, 699, 1147, 1507
BstSCI CCNGG 4 cut(s) 162, 604, 633, 690
BstSLI GKGCMC 3 cut(s) 378, 1133, 1180
BstV1I GCAGC 2 cut(s) 18, 616
BstV2I GAAGAC 2 cut(s) 774, 854
BstX2I RGATCY 2 cut(s) 621, 640
BstXI CCANNNNNNTGG 1 cut(s) 1044
BstYI RGATCY 2 cut(s) 621, 640
BstZI CGGCCG 1 cut(s) 1103
BtsCI GGATG 4 cut(s) 114, 239, 250, 1455
BtsIMutI CAGTG 3 cut(s) 559, 1044, 1137
Cac8I GCNNGC 6 cut(s) 378, 569, 669, 688, 981, 985
CfoI GCGC 2 cut(s) 667, 1223
Cfr10I RCCGGY 2 cut(s) 590, 600
Cfr13I GGNCC 6 cut(s) 74, 476, 603, 919, 965, 977
CseI GACGC 2 cut(s) 759, 1006
Csp6I GTAC 3 cut(s) 594, 909, 1534
CspAI ACCGGT 1 cut(s) 590
CviQI GTAC 3 cut(s) 594, 909, 1534
DdeI CTNAG 3 cut(s) 352, 806, 1515
DpnI GATC 6 cut(s) 39, 362, 623, 642, 1101, 1382
DpnII GATC 6 cut(s) 37, 360, 621, 640, 1099, 1380
DrdI GACNNNNNNGTC 1 cut(s) 434
DseDI GACNNNNNNGTC 1 cut(s) 434
EaeI YGGCCR 2 cut(s) 489, 1103
EagI CGGCCG 1 cut(s) 1103
Eam1104I CTCTTC 1 cut(s) 1388
EarI CTCTTC 1 cut(s) 1388
EciI GGCGGA 1 cut(s) 653
EclXI CGGCCG 1 cut(s) 1103
Eco130I CCWWGG 3 cut(s) 895, 992, 1002
Eco31I GGTCTC 1 cut(s) 1447
Eco47I GGWCC 1 cut(s) 919
Eco52I CGGCCG 1 cut(s) 1103
EcoO109I RGGNCCY 2 cut(s) 476, 977
EcoRII CCWGG 3 cut(s) 162, 633, 690
EcoT14I CCWWGG 3 cut(s) 895, 992, 1002
EcoT22I ATGCAT 1 cut(s) 673
ErhI CCWWGG 3 cut(s) 895, 992, 1002
FaqI GGGAC 2 cut(s) 968, 1465
FauI CCCGC 1 cut(s) 695
FbaI TGATCA 1 cut(s) 360
FblI GTMKAC 1 cut(s) 1368
Fnu4HI GCNGC 2 cut(s) 7, 630
FokI GGATG 4 cut(s) 121, 246, 257, 1462
Fsp4HI GCNGC 2 cut(s) 7, 630
FspBI CTAG 3 cut(s) 564, 1173, 1257
FspI TGCGCA 1 cut(s) 1222
GlaI GCGC 2 cut(s) 666, 1222
GluI GCNGC 2 cut(s) 7, 630
HapII CCGG 4 cut(s) 430, 591, 601, 606
HgaI GACGC 2 cut(s) 759, 1006
HhaI GCGC 2 cut(s) 667, 1223
Hin1I GRCGYC 1 cut(s) 998
Hin6I GCGC 2 cut(s) 665, 1221
HinP1I GCGC 2 cut(s) 665, 1221
HincII GTYRAC 1 cut(s) 836
HindII GTYRAC 1 cut(s) 836
HinfI GANTC 2 cut(s) 1027, 1385
HpaII CCGG 4 cut(s) 430, 591, 601, 606
HphI GGTGA 6 cut(s) 190, 262, 772, 884, 1093, 1421
Hpy166II GTNNAC 7 cut(s) 145, 376, 836, 919, 1131, 1327, 1369
Hpy188I TCNGA 6 cut(s) 226, 619, 715, 1032, 1249, 1518
Hpy188III TCNNGA 3 cut(s) 41, 1435, 1546
Hpy8I GTNNAC 7 cut(s) 145, 376, 836, 919, 1131, 1327, 1369
Hpy99I CGWCG 2 cut(s) 743, 753
HpyAV CCTTC 4 cut(s) 98, 814, 1178, 1342
HpyCH4III ACNGT 4 cut(s) 124, 784, 923, 1126
HpyCH4IV ACGT 1 cut(s) 1338
HpyF10VI GCNNNNNNNGC 6 cut(s) 635, 692, 701, 800, 1301, 1328
HpyF3I CTNAG 3 cut(s) 352, 806, 1515
HpySE526I ACGT 1 cut(s) 1338
Hsp92I GRCGYC 1 cut(s) 998
HspAI GCGC 2 cut(s) 665, 1221
Ksp22I TGATCA 1 cut(s) 360
Kzo9I GATC 6 cut(s) 37, 360, 621, 640, 1099, 1380
LmnI GCTCC 2 cut(s) 675, 992
Lsp1109I GCAGC 2 cut(s) 18, 616
LweI GCATC 2 cut(s) 102, 217
MaeI CTAG 3 cut(s) 564, 1173, 1257
MaeII ACGT 1 cut(s) 1338
MaeIII GTNAC 4 cut(s) 212, 421, 463, 1081
MalI GATC 6 cut(s) 39, 362, 623, 642, 1101, 1382
MboI GATC 6 cut(s) 37, 360, 621, 640, 1099, 1380
MboII GAAGA 6 cut(s) 25, 125, 213, 779, 859, 1405
MfeI CAATTG 1 cut(s) 829
MflI RGATCY 2 cut(s) 621, 640
MhlI GDGCHC 4 cut(s) 378, 573, 1133, 1180
MlsI TGGCCA 1 cut(s) 491
MluNI TGGCCA 1 cut(s) 491
MlyI GAGTC 2 cut(s) 1036, 1394
MmeI TCCRAC 2 cut(s) 990, 1010
Mox20I TGGCCA 1 cut(s) 491
Mph1103I ATGCAT 1 cut(s) 673
MscI TGGCCA 1 cut(s) 491
MseI TTAA 4 cut(s) 290, 333, 341, 441
MslI CAYNNNNRTG 4 cut(s) 552, 584, 1142, 1448
Msp20I TGGCCA 1 cut(s) 491
MspA1I CMGCKG 1 cut(s) 629
MspI CCGG 4 cut(s) 430, 591, 601, 606
MspR9I CCNGG 4 cut(s) 164, 606, 635, 692
MunI CAATTG 1 cut(s) 829
MvaI CCWGG 3 cut(s) 164, 635, 692
MwoI GCNNNNNNNGC 6 cut(s) 635, 692, 701, 800, 1301, 1328
NciI CCSGG 1 cut(s) 606
NdeII GATC 6 cut(s) 37, 360, 621, 640, 1099, 1380
NlaIV GGNNCC 5 cut(s) 478, 677, 825, 901, 978
NmeAIII GCCGAG 1 cut(s) 1131
NmuCI GTSAC 1 cut(s) 1081
NsbI TGCGCA 1 cut(s) 1222
NsiI ATGCAT 1 cut(s) 673
NspI RCATGY 4 cut(s) 671, 699, 1147, 1507
NspV TTCGAA 1 cut(s) 525
OliI CACNNNNGTG 1 cut(s) 552
PaeI GCATGC 1 cut(s) 671
PcsI WCGNNNNNNNCGW 1 cut(s) 1335
PfoI TCCNGGA 1 cut(s) 162
PinAI ACCGGT 1 cut(s) 590
PkrI GCNGC 2 cut(s) 8, 631
PleI GAGTC 2 cut(s) 1035, 1393
PpsI GAGTC 2 cut(s) 1035, 1393
PshBI ATTAAT 1 cut(s) 290
Psp6I CCWGG 3 cut(s) 162, 633, 690
PspGI CCWGG 3 cut(s) 162, 633, 690
PspN4I GGNNCC 5 cut(s) 478, 677, 825, 901, 978
PspPI GGNCC 6 cut(s) 74, 476, 603, 919, 965, 977
PsuI RGATCY 2 cut(s) 621, 640
RsaI GTAC 3 cut(s) 595, 910, 1535
RsaNI GTAC 3 cut(s) 594, 909, 1534
RseI CAYNNNNRTG 4 cut(s) 552, 584, 1142, 1448
SaqAI TTAA 4 cut(s) 290, 333, 341, 441
SatI GCNGC 2 cut(s) 7, 630
Sau3AI GATC 6 cut(s) 37, 360, 621, 640, 1099, 1380
Sau96I GGNCC 6 cut(s) 74, 476, 603, 919, 965, 977
ScaI AGTACT 1 cut(s) 910
SchI GAGTC 2 cut(s) 1036, 1394
ScrFI CCNGG 4 cut(s) 164, 606, 635, 692
SduI GDGCHC 4 cut(s) 378, 573, 1133, 1180
SetI ASST 9 cut(s) 135, 198, 777, 854, 929, 939, 989, 1189, 1341
SfaNI GCATC 2 cut(s) 102, 217
SfuI TTCGAA 1 cut(s) 525
SinI GGWCC 1 cut(s) 919
SmiMI CAYNNNNRTG 4 cut(s) 552, 584, 1142, 1448
SphI GCATGC 1 cut(s) 671
SsiI CCGC 4 cut(s) 627, 638, 688, 1283
SspI AATATT 1 cut(s) 889
SspMI CTAG 3 cut(s) 564, 1173, 1257
StyD4I CCNGG 4 cut(s) 162, 604, 633, 690
StyI CCWWGG 3 cut(s) 895, 992, 1002
TaaI ACNGT 4 cut(s) 124, 784, 923, 1126
TaiI ACGT 1 cut(s) 1341
TaqI TCGA 8 cut(s) 36, 96, 525, 738, 1089, 1291, 1383, 1388
TaqII GACCGA 1 cut(s) 1471
TatI WGTACW 1 cut(s) 908
Tru1I TTAA 4 cut(s) 290, 333, 341, 441
Tru9I TTAA 4 cut(s) 290, 333, 341, 441
TscAI CASTG 3 cut(s) 559, 1044, 1144
TseFI GTSAC 1 cut(s) 1081
TseI GCWGC 2 cut(s) 6, 629
Tsp45I GTSAC 1 cut(s) 1081
TspDTI ATGAA 8 cut(s) 26, 206, 229, 256, 266, 383, 420, 1406
TspGWI ACGGA 1 cut(s) 779
TspRI CASTG 3 cut(s) 559, 1044, 1144
VneI GTGCAC 2 cut(s) 374, 1129
VpaK11BI GGWCC 1 cut(s) 919
VspI ATTAAT 1 cut(s) 290
XapI RAATTY 1 cut(s) 1424
XceI RCATGY 4 cut(s) 671, 699, 1147, 1507
XmiI GTMKAC 1 cut(s) 1368
XspI CTAG 3 cut(s) 564, 1173, 1257
ZrmI AGTACT 1 cut(s) 910
Zsp2I ATGCAT 1 cut(s) 673
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.