pycom08g13260

hydroxyproline O-arabinosyltransferase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0013842
Physical Location & Seq
Reverse (-)
11874875 .. 11876818
1944 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 831 bp
ATGTACTACTGGTTCAAGAAGTTTCAGAACGAACCCGGCTCCGAGATGGGCGGGTTCACTAGGATCTTGCACAATGGGAAGCCGGACAAGTACATGGATGAAATCCCTACTTTTATTGCTCAGCCTTTGCCTTCTGGAATGGATCGGGGTTATATTGTTCTCAACCGACCATGGGCATTTGTGCAATGGCTTCAGCAAGCAGACATCAAAGAAGATTACATACTGATGTCAGAGCCCGATCATGTAATTGTCAAGCCAATACCGAACTTGTCTACTGATGGGCTTGGAGCTGCATTTCCTTTCTTCTACATTGAACCGAAAAAGTACGAGTCAGTCCTCCGGAAATACTTCCCTGAGGACAAGGGACCAATAACTAAAATTGATCCAATTGGAAATTCTCCTGTCATTGTTGGGAAGGAATCTCTCAAGAAAATTGCTCCCACGTGGATGAATGTTTCTTTGGCAATGAAGAAGGATCCTGAGACAGATAAAGCTTTTGGTTGGGTTCTTGAAATGTATGCTTATGCTGTTGCATCTGCTCACCATGGGGTTGGTAATATCTTATACAAGGACTTCATGCTTCAGCCTCCGTGGGATAAAAAAATTGGCAAGAAGTTCATAATTCATTACACTTATGGATGTGACTATAATATGAAGGGTGAACTAACATATGGGAAAATCGGAGAATGGAGATTTGATAAAAGGTCTTATGATACTGTCGCACCGCCTAGAAACCTTACTATGCCTCCACATGGTGTTCCAGAAAGCGTGGTGACCCTGGTGAAAATGGTGAATGAAGCCACAGCAAACATTCCAAAGTGGGGGGAATAG

Protein Analysis

277

Amino Acids

31.64

Weight (kDa)

8.33

Isoelectric Point (pI)

45.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HPAT PF23452 1 - 274 1.9e-118 Hydroxyproline O-arabinosyltransferase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013842)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G25265
fragaria_vesca FvH4_2g28340
malus_domestica MD08G1153200.v1.1 MD15G1130300.v1.1
prunus_persica Prupe.1G485800_v2.0.a1
pyrus_communis pycom08g13260 pycom15g11750
rosa_chinensis RchiOBHm_Chr6g0297261
rosa_laevigata RLG00000011616
rosa_multiflora Rmu_sc0004535.1_g000002 Rmu_sc0007115.1_g000001
rosa_roxburghii Rroxscaffold_7G00170700
rosa_rugosa Rorug06G0270100
rosa_samantha Rh6BG389600 Rh6CG394800 Rh6DG381500
rosa_wichuraiana Rw6G033240

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 272
AccIII TCCGGA 1 cut(s) 339
AciI CCGC 2 cut(s) 51, 725
AclWI GGATC 5 cut(s) 71, 150, 377, 470, 483
AcsI RAATTY 1 cut(s) 394
AcuI CTGAAG 2 cut(s) 176, 566
AcvI CACGTG 1 cut(s) 444
AdeI CACNNNGTG 1 cut(s) 755
AfaI GTAC 3 cut(s) 5, 92, 326
AfiI CCNNNNNNNGG 3 cut(s) 172, 752, 821
AgsI TTSAA 3 cut(s) 16, 314, 512
AjnI CCWGG 1 cut(s) 777
AjuI GAANNNNNNNTTGG 4 cut(s) 379, 411, 443, 475
AluBI AGCT 2 cut(s) 290, 494
AluI AGCT 2 cut(s) 290, 494
Alw26I GTCTC 1 cut(s) 476
AlwI GGATC 5 cut(s) 71, 150, 377, 470, 483
Aor13HI TCCGGA 1 cut(s) 339
ApeKI GCWGC 1 cut(s) 290
ApoI RAATTY 1 cut(s) 394
Asp700I GAANNNNTTC 1 cut(s) 347
AspS9I GGNCC 1 cut(s) 365
AsuC2I CCSGG 1 cut(s) 36
AsuHPI GGTGA 5 cut(s) 533, 671, 784, 793, 802
AvaII GGWCC 1 cut(s) 365
AxyI CCTNAGG 1 cut(s) 354
BamHI GGATCC 1 cut(s) 475
BanII GRGCYC 1 cut(s) 237
BarI GAAGNNNNNNTAC 2 cut(s) 204, 236
BbrPI CACGTG 1 cut(s) 444
BbvI GCAGC 1 cut(s) 277
BccI CCATC 2 cut(s) 40, 272
BciT130I CCWGG 1 cut(s) 779
BcnI CCSGG 1 cut(s) 36
BcoDI GTCTC 1 cut(s) 476
BfaI CTAG 2 cut(s) 60, 729
BisI GCNGC 1 cut(s) 291
BlpI GCTNAGC 1 cut(s) 120
BlsI GCNGC 1 cut(s) 292
Bme1390I CCNGG 2 cut(s) 36, 779
Bme18I GGWCC 1 cut(s) 365
BmgT120I GGNCC 1 cut(s) 365
BmiI GGNNCC 3 cut(s) 40, 366, 477
BmrFI CCNGG 2 cut(s) 36, 779
BmsI GCATC 1 cut(s) 542
Bpu1102I GCTNAGC 1 cut(s) 120
BpuEI CTTGAG 1 cut(s) 410
BpuMI CCSGG 1 cut(s) 36
BsaAI YACGTR 1 cut(s) 444
BsaJI CCNNGG 4 cut(s) 170, 544, 590, 777
BsaWI WCCGGW 1 cut(s) 339
BsaXI ACNNNNNCTCC 2 cut(s) 730, 760
Bsc4I CCNNNNNNNGG 3 cut(s) 172, 752, 821
Bse1I ACTGG 1 cut(s) 14
Bse21I CCTNAGG 1 cut(s) 354
Bse3DI GCAATG 2 cut(s) 191, 471
BseAI TCCGGA 1 cut(s) 339
BseBI CCWGG 1 cut(s) 779
BseDI CCNNGG 4 cut(s) 170, 544, 590, 777
BseGI GGATG 3 cut(s) 103, 453, 644
BseLI CCNNNNNNNGG 3 cut(s) 172, 752, 821
BseMI GCAATG 2 cut(s) 191, 471
BseMII CTCAG 3 cut(s) 134, 345, 471
BseNI ACTGG 1 cut(s) 14
BseXI GCAGC 1 cut(s) 277
BsiSI CCGG 3 cut(s) 36, 83, 340
BslFI GGGAC 1 cut(s) 378
BslI CCNNNNNNNGG 3 cut(s) 172, 752, 821
BsmAI GTCTC 1 cut(s) 476
BsmFI GGGAC 1 cut(s) 378
Bsp1286I GDGCHC 1 cut(s) 237
Bsp13I TCCGGA 1 cut(s) 339
Bsp143I GATC 5 cut(s) 63, 142, 238, 382, 475
Bsp1720I GCTNAGC 1 cut(s) 120
Bsp19I CCATGG 2 cut(s) 170, 544
BspACI CCGC 2 cut(s) 51, 725
BspCNI CTCAG 3 cut(s) 133, 346, 472
BspEI TCCGGA 1 cut(s) 339
BspLI GGNNCC 3 cut(s) 40, 366, 477
BspPI GGATC 5 cut(s) 71, 150, 377, 470, 483
BsrDI GCAATG 2 cut(s) 191, 471
BsrI ACTGG 1 cut(s) 14
BssECI CCNNGG 4 cut(s) 170, 544, 590, 777
BssMI GATC 5 cut(s) 63, 142, 238, 382, 475
BssT1I CCWWGG 2 cut(s) 170, 544
Bst2UI CCWGG 1 cut(s) 779
Bst4CI ACNGT 1 cut(s) 718
BstBAI YACGTR 1 cut(s) 444
BstC8I GCNNGC 1 cut(s) 198
BstDEI CTNAG 3 cut(s) 120, 354, 480
BstDSI CCRYGG 3 cut(s) 170, 544, 590
BstEII GGTNACC 1 cut(s) 772
BstF5I GGATG 3 cut(s) 103, 453, 644
BstKTI GATC 5 cut(s) 66, 145, 241, 385, 478
BstMAI GTCTC 1 cut(s) 476
BstMBI GATC 5 cut(s) 63, 142, 238, 382, 475
BstNI CCWGG 1 cut(s) 779
BstPI GGTNACC 1 cut(s) 772
BstSCI CCNGG 2 cut(s) 34, 777
BstV1I GCAGC 1 cut(s) 277
BstX2I RGATCY 2 cut(s) 63, 475
BstXI CCANNNNNNTGG 1 cut(s) 551
BstYI RGATCY 2 cut(s) 63, 475
Bsu36I CCTNAGG 1 cut(s) 354
BtgI CCRYGG 3 cut(s) 170, 544, 590
BtsCI GGATG 3 cut(s) 103, 453, 644
Cac8I GCNNGC 1 cut(s) 198
Cfr13I GGNCC 1 cut(s) 365
Csp6I GTAC 3 cut(s) 4, 91, 325
CviAII CATG 6 cut(s) 94, 171, 242, 545, 577, 752
CviQI GTAC 3 cut(s) 4, 91, 325
DdeI CTNAG 3 cut(s) 120, 354, 480
DpnI GATC 5 cut(s) 65, 144, 240, 384, 477
DpnII GATC 5 cut(s) 63, 142, 238, 382, 475
DraIII CACNNNGTG 1 cut(s) 755
Eco130I CCWWGG 2 cut(s) 170, 544
Eco24I GRGCYC 1 cut(s) 237
Eco47I GGWCC 1 cut(s) 365
Eco57I CTGAAG 2 cut(s) 176, 566
Eco72I CACGTG 1 cut(s) 444
Eco81I CCTNAGG 1 cut(s) 354
Eco91I GGTNACC 1 cut(s) 772
EcoO65I GGTNACC 1 cut(s) 772
EcoRII CCWGG 1 cut(s) 777
EcoT14I CCWWGG 2 cut(s) 170, 544
EcoT38I GRGCYC 1 cut(s) 237
ErhI CCWWGG 2 cut(s) 170, 544
FaeI CATG 6 cut(s) 97, 174, 245, 548, 580, 755
FaqI GGGAC 1 cut(s) 378
FatI CATG 6 cut(s) 93, 170, 241, 544, 576, 751
FauI CCCGC 1 cut(s) 44
FauNDI CATATG 1 cut(s) 670
FblI GTMKAC 1 cut(s) 272
Fnu4HI GCNGC 1 cut(s) 291
FokI GGATG 3 cut(s) 110, 460, 651
FriOI GRGCYC 1 cut(s) 237
Fsp4HI GCNGC 1 cut(s) 291
FspBI CTAG 2 cut(s) 60, 729
GluI GCNGC 1 cut(s) 291
HapII CCGG 3 cut(s) 36, 83, 340
Hin1II CATG 6 cut(s) 97, 174, 245, 548, 580, 755
HindIII AAGCTT 1 cut(s) 492
HinfI GANTC 2 cut(s) 329, 419
HpaII CCGG 3 cut(s) 36, 83, 340
HphI GGTGA 5 cut(s) 533, 671, 784, 793, 802
Hpy166II GTNNAC 3 cut(s) 57, 273, 662
Hpy188I TCNGA 4 cut(s) 27, 43, 232, 683
Hpy188III TCNNGA 7 cut(s) 16, 135, 340, 427, 479, 509, 761
Hpy8I GTNNAC 3 cut(s) 57, 273, 662
HpyAV CCTTC 4 cut(s) 141, 409, 466, 649
HpyCH4III ACNGT 1 cut(s) 718
HpyCH4IV ACGT 1 cut(s) 443
HpyCH4V TGCA 4 cut(s) 70, 184, 293, 533
HpyF3I CTNAG 3 cut(s) 120, 354, 480
HpySE526I ACGT 1 cut(s) 443
Hsp92II CATG 6 cut(s) 97, 174, 245, 548, 580, 755
Kpn2I TCCGGA 1 cut(s) 339
Kzo9I GATC 5 cut(s) 63, 142, 238, 382, 475
LmnI GCTCC 3 cut(s) 44, 287, 442
Lsp1109I GCAGC 1 cut(s) 277
LweI GCATC 1 cut(s) 542
MaeI CTAG 2 cut(s) 60, 729
MaeII ACGT 1 cut(s) 443
MaeIII GTNAC 2 cut(s) 641, 772
MalI GATC 5 cut(s) 65, 144, 240, 384, 477
MboI GATC 5 cut(s) 63, 142, 238, 382, 475
MboII GAAGA 3 cut(s) 224, 295, 481
MfeI CAATTG 1 cut(s) 387
MflI RGATCY 2 cut(s) 63, 475
MhlI GDGCHC 1 cut(s) 237
MluCI AATT 7 cut(s) 246, 378, 387, 394, 432, 603, 621
MlyI GAGTC 1 cut(s) 338
MnlI CCTC 4 cut(s) 347, 349, 597, 756
MroI TCCGGA 1 cut(s) 339
MroXI GAANNNNTTC 1 cut(s) 347
MslI CAYNNNNRTG 2 cut(s) 224, 446
MspI CCGG 3 cut(s) 36, 83, 340
MspR9I CCNGG 2 cut(s) 36, 779
MunI CAATTG 1 cut(s) 387
MvaI CCWGG 1 cut(s) 779
NciI CCSGG 1 cut(s) 36
NcoI CCATGG 2 cut(s) 170, 544
NdeI CATATG 1 cut(s) 670
NdeII GATC 5 cut(s) 63, 142, 238, 382, 475
NlaIII CATG 6 cut(s) 97, 174, 245, 548, 580, 755
NlaIV GGNNCC 3 cut(s) 40, 366, 477
NmuCI GTSAC 2 cut(s) 641, 772
PdmI GAANNNNTTC 1 cut(s) 347
PfeI GAWTC 1 cut(s) 419
PkrI GCNGC 1 cut(s) 292
PleI GAGTC 1 cut(s) 337
PmaCI CACGTG 1 cut(s) 444
PmlI CACGTG 1 cut(s) 444
PpsI GAGTC 1 cut(s) 337
Ppu21I YACGTR 1 cut(s) 444
Psp6I CCWGG 1 cut(s) 777
PspCI CACGTG 1 cut(s) 444
PspEI GGTNACC 1 cut(s) 772
PspGI CCWGG 1 cut(s) 777
PspN4I GGNNCC 3 cut(s) 40, 366, 477
PspPI GGNCC 1 cut(s) 365
PsrI GAACNNNNNNTAC 1 cut(s) 28
PsuI RGATCY 2 cut(s) 63, 475
RsaI GTAC 3 cut(s) 5, 92, 326
RsaNI GTAC 3 cut(s) 4, 91, 325
RseI CAYNNNNRTG 2 cut(s) 224, 446
SatI GCNGC 1 cut(s) 291
Sau3AI GATC 5 cut(s) 63, 142, 238, 382, 475
Sau96I GGNCC 1 cut(s) 365
SchI GAGTC 1 cut(s) 338
ScrFI CCNGG 2 cut(s) 36, 779
SduI GDGCHC 1 cut(s) 237
SetI ASST 5 cut(s) 292, 446, 496, 707, 738
SfaNI GCATC 1 cut(s) 542
SinI GGWCC 1 cut(s) 365
SmiMI CAYNNNNRTG 2 cut(s) 224, 446
SmlI CTYRAG 1 cut(s) 425
SmoI CTYRAG 1 cut(s) 425
Sse9I AATT 7 cut(s) 246, 378, 387, 394, 432, 603, 621
SsiI CCGC 2 cut(s) 51, 725
SspMI CTAG 2 cut(s) 60, 729
StyD4I CCNGG 2 cut(s) 34, 777
StyI CCWWGG 2 cut(s) 170, 544
TaaI ACNGT 1 cut(s) 718
TaiI ACGT 1 cut(s) 446
TasI AATT 7 cut(s) 246, 378, 387, 394, 432, 603, 621
TatI WGTACW 2 cut(s) 3, 90
TfiI GAWTC 1 cut(s) 419
TseFI GTSAC 2 cut(s) 641, 772
TseI GCWGC 1 cut(s) 290
Tsp45I GTSAC 2 cut(s) 641, 772
TspDTI ATGAA 8 cut(s) 114, 464, 482, 565, 607, 614, 668, 810
TspGWI ACGGA 1 cut(s) 579
VpaK11BI GGWCC 1 cut(s) 365
XapI RAATTY 1 cut(s) 394
XmiI GTMKAC 1 cut(s) 272
XmnI GAANNNNTTC 1 cut(s) 347
XspI CTAG 2 cut(s) 60, 729
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.