pycom08g14200

WAT1-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0013852
Physical Location & Seq
Reverse (-)
13263304 .. 13265642
2339 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1005 bp
ATGGGAGATGATGAGCAGAACAACTCAGGTGGGAGGTTTCGTGTGATGATGAAGAAGCTGAAACCTTATTTGCTGGTGGTGAGCTTGCAATTTGGGAGTGCAGGGATGTACATAATCAGCATGGCCACTCTCAATCATGGCATGAATCGATATGTTCTCATCGTCTATCGCAATGCTACTGCAGCACTTGTCCTCGCTCCTTTTGCCTTGGTTCTTGAAAGGGTCGAAACTGTAAAAATCAAAGAGGTGCGAAGTCAAGCCAAGGTGATAGGAACCCTAGTAACCTTTGCTGGTGCTTTGCTGATGACACTGTACAAAGGCCCTGTCATTGACCTCCTGTGGTCGCACAAAACCAGCAGCCACCATGAAAGTAGCAGCACCACTATAACCTCCAACCAACACTGGATAGCCGGGACACTCTTTATCCTCCTGGGTAGTGTTGCCTGGTCCTGTTTCTACGTATTGCAATCTATAACAGTGAAGAAATACCCAGCAAACATCTCACTTTCCTGCTTTATATGCTTGGCGGGAGCGTTGCAAGGCTCAGTTGTAGCATTCACTGTAGAGCGTTGCCCTAGTGCATGGGCGGTTGGATGGGATTCAAGGCTCCTTGCACCTCTCTACACGGGACTAATTAGCTCTGGGATTACATATTACGTGCAAGGCTTGGTTATGAAGACTAGAGGCCCGGTTTTTGTAACGGCTTTTAACCCTCTCTGCATGATTATCGTGTCCATTCTGGGTTCCATTATTCTAGCTGAGAAACTTCACCTTGGCAGTATCATTGGAGGCATGGTCATCGCATTTGGCCTTTATTCTGTGGTGTGGGGTAAAAGCAAGGATTATGGCACTAACCCAGCTGTGCAATCCAATACTACTGTGAAGGATGAAGCTCAGGAGTTACCAATTATTAATGCAAACGCTATCAGTGGTGCAAAATTGGTGATTGATAAAAATAGCATCGACCAGCCATCCCAAATGCAATTAAAGACTGTCCAAATATAA

Protein Analysis

335

Amino Acids

36.26

Weight (kDa)

9.35

Isoelectric Point (pI)

31.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EamA PF00892 138 - 275 5.1e-09 EamA-like transporter family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013852)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G08290 AT4G08290
fragaria_vesca FvH4_2g26690 FvH4_2g26690
prunus_persica Prupe.1G500600_v2.0.a1
pyrus_communis pycom08g14200
rosa_chinensis RchiOBHm_Chr6g0295531
rosa_laevigata RLG00000011793
rosa_multiflora Rmu_sc0005000.1_g000013
rosa_roxburghii Rroxscaffold_7G00172390
rosa_rugosa Rorug06G0251700
rosa_samantha Rh2AG083600 Rh6AG364900 Rh6BG373300 Rh6CG379500 Rh6DG366000
rosa_wichuraiana Rw6G031870

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 527, 587
AcoI YGGCCR 1 cut(s) 123
AfaI GTAC 2 cut(s) 110, 314
AgsI TTSAA 2 cut(s) 218, 603
AjnI CCWGG 2 cut(s) 429, 443
AluBI AGCT 6 cut(s) 58, 84, 639, 758, 860, 893
AluI AGCT 6 cut(s) 58, 84, 639, 758, 860, 893
AoxI GGCC 4 cut(s) 123, 319, 685, 808
ApeKI GCWGC 3 cut(s) 182, 357, 375
ArsI GACNNNNNNTTYG 2 cut(s) 309, 341
AseI ATTAAT 1 cut(s) 912
AspS9I GGNCC 3 cut(s) 320, 447, 686
AsuC2I CCSGG 2 cut(s) 412, 689
AsuHPI GGTGA 4 cut(s) 91, 277, 761, 955
AvaII GGWCC 1 cut(s) 447
BalI TGGCCA 1 cut(s) 125
BbsI GAAGAC 1 cut(s) 683
BbvI GCAGC 3 cut(s) 194, 369, 387
BccI CCATC 2 cut(s) 588, 979
BceAI ACGGC 1 cut(s) 717
BcgI CGANNNNNNTGC 4 cut(s) 709, 743, 781, 815
BciT130I CCWGG 2 cut(s) 431, 445
BcnI CCSGG 2 cut(s) 412, 689
BfaI CTAG 4 cut(s) 278, 576, 681, 755
BfmI CTRYAG 2 cut(s) 180, 561
BisI GCNGC 3 cut(s) 183, 358, 376
BlsI GCNGC 3 cut(s) 184, 359, 377
Bme1390I CCNGG 4 cut(s) 412, 431, 445, 689
Bme18I GGWCC 1 cut(s) 447
BmgT120I GGNCC 3 cut(s) 320, 447, 686
BmiI GGNNCC 3 cut(s) 274, 608, 745
BmrFI CCNGG 4 cut(s) 412, 431, 445, 689
BmsI GCATC 1 cut(s) 969
BpiI GAAGAC 1 cut(s) 683
Bpu10I CCTNAGC 1 cut(s) 894
BpuMI CCSGG 2 cut(s) 412, 689
Bsa29I ATCGAT 1 cut(s) 148
BsaAI YACGTR 2 cut(s) 460, 658
BsaJI CCNNGG 4 cut(s) 207, 261, 430, 772
BsaXI ACNNNNNCTCC 4 cut(s) 25, 55, 780, 810
Bse1I ACTGG 1 cut(s) 407
Bse3DI GCAATG 1 cut(s) 178
BseBI CCWGG 2 cut(s) 431, 445
BseCI ATCGAT 1 cut(s) 148
BseDI CCNNGG 4 cut(s) 207, 261, 430, 772
BseGI GGATG 4 cut(s) 111, 599, 892, 971
BseMI GCAATG 1 cut(s) 178
BseMII CTCAG 4 cut(s) 39, 558, 750, 908
BseNI ACTGG 1 cut(s) 407
BseXI GCAGC 3 cut(s) 194, 369, 387
BseYI CCCAGC 2 cut(s) 490, 856
BsgI GTGCAG 1 cut(s) 120
BshFI GGCC 4 cut(s) 125, 321, 687, 810
BshVI ATCGAT 1 cut(s) 148
BsiSI CCGG 2 cut(s) 411, 689
BslFI GGGAC 2 cut(s) 427, 642
BsmFI GGGAC 2 cut(s) 427, 642
BsmI GAATGC 1 cut(s) 554
BsnI GGCC 4 cut(s) 125, 321, 687, 810
Bsp1407I TGTACA 2 cut(s) 108, 312
BspACI CCGC 2 cut(s) 527, 587
BspANI GGCC 4 cut(s) 125, 321, 687, 810
BspCNI CTCAG 4 cut(s) 38, 557, 751, 907
BspDI ATCGAT 1 cut(s) 148
BspLI GGNNCC 3 cut(s) 274, 608, 745
BspMAI CTGCAG 1 cut(s) 184
BsrDI GCAATG 1 cut(s) 178
BsrGI TGTACA 2 cut(s) 108, 312
BsrI ACTGG 1 cut(s) 407
BssECI CCNNGG 4 cut(s) 207, 261, 430, 772
BssT1I CCWWGG 3 cut(s) 207, 261, 772
Bst2UI CCWGG 2 cut(s) 431, 445
Bst4CI ACNGT 6 cut(s) 232, 312, 478, 562, 880, 994
BstAUI TGTACA 2 cut(s) 108, 312
BstBAI YACGTR 2 cut(s) 460, 658
BstC8I GCNNGC 1 cut(s) 86
BstDEI CTNAG 4 cut(s) 25, 544, 759, 894
BstF5I GGATG 4 cut(s) 111, 599, 892, 971
BstMWI GCNNNNNNNGC 3 cut(s) 182, 203, 519
BstNI CCWGG 2 cut(s) 431, 445
BstSCI CCNGG 4 cut(s) 410, 429, 443, 687
BstSFI CTRYAG 2 cut(s) 180, 561
BstSNI TACGTA 1 cut(s) 460
BstV1I GCAGC 3 cut(s) 194, 369, 387
BstV2I GAAGAC 1 cut(s) 683
Bsu15I ATCGAT 1 cut(s) 148
BsuRI GGCC 4 cut(s) 125, 321, 687, 810
BsuTUI ATCGAT 1 cut(s) 148
BtgZI GCGATG 1 cut(s) 784
BtsCI GGATG 4 cut(s) 111, 599, 892, 971
BtsIMutI CAGTG 5 cut(s) 308, 400, 483, 558, 934
Cac8I GCNNGC 1 cut(s) 86
Cfr13I GGNCC 3 cut(s) 320, 447, 686
ClaI ATCGAT 1 cut(s) 148
Csp6I GTAC 2 cut(s) 109, 313
CspCI CAANNNNNGTGG 2 cut(s) 10, 45
CviAII CATG 7 cut(s) 121, 137, 142, 365, 582, 721, 793
CviQI GTAC 2 cut(s) 109, 313
DdeI CTNAG 4 cut(s) 25, 544, 759, 894
EaeI YGGCCR 1 cut(s) 123
Eco105I TACGTA 1 cut(s) 460
Eco130I CCWWGG 3 cut(s) 207, 261, 772
Eco47I GGWCC 1 cut(s) 447
EcoO109I RGGNCCY 1 cut(s) 320
EcoRII CCWGG 2 cut(s) 429, 443
EcoT14I CCWWGG 3 cut(s) 207, 261, 772
ErhI CCWWGG 3 cut(s) 207, 261, 772
FaeI CATG 7 cut(s) 124, 140, 145, 368, 585, 724, 796
FaqI GGGAC 2 cut(s) 427, 642
FatI CATG 7 cut(s) 120, 136, 141, 364, 581, 720, 792
FauI CCCGC 1 cut(s) 520
Fnu4HI GCNGC 3 cut(s) 183, 358, 376
FokI GGATG 4 cut(s) 118, 606, 899, 958
Fsp4HI GCNGC 3 cut(s) 183, 358, 376
FspBI CTAG 4 cut(s) 278, 576, 681, 755
GluI GCNGC 3 cut(s) 183, 358, 376
GsaI CCCAGC 2 cut(s) 494, 860
HaeIII GGCC 4 cut(s) 125, 321, 687, 810
HapII CCGG 2 cut(s) 411, 689
Hin1II CATG 7 cut(s) 124, 140, 145, 368, 585, 724, 796
HinfI GANTC 2 cut(s) 145, 599
HpaII CCGG 2 cut(s) 411, 689
HphI GGTGA 4 cut(s) 91, 277, 761, 955
Hpy188III TCNNGA 2 cut(s) 215, 896
HpyAV CCTTC 1 cut(s) 877
HpyCH4III ACNGT 6 cut(s) 232, 312, 478, 562, 880, 994
HpyCH4IV ACGT 2 cut(s) 459, 657
HpyF10VI GCNNNNNNNGC 3 cut(s) 182, 203, 519
HpyF3I CTNAG 4 cut(s) 25, 544, 759, 894
HpySE526I ACGT 2 cut(s) 459, 657
Hsp92II CATG 7 cut(s) 124, 140, 145, 368, 585, 724, 796
LmnI GCTCC 3 cut(s) 202, 530, 612
Lsp1109I GCAGC 3 cut(s) 194, 369, 387
LweI GCATC 1 cut(s) 969
MaeI CTAG 4 cut(s) 278, 576, 681, 755
MaeII ACGT 2 cut(s) 459, 657
MaeIII GTNAC 3 cut(s) 280, 697, 900
MboII GAAGA 3 cut(s) 64, 493, 688
MlsI TGGCCA 1 cut(s) 125
MluCI AATT 5 cut(s) 89, 633, 906, 938, 983
MluNI TGGCCA 1 cut(s) 125
MmeI TCCRAC 2 cut(s) 417, 571
Mox20I TGGCCA 1 cut(s) 125
MscI TGGCCA 1 cut(s) 125
MseI TTAA 3 cut(s) 708, 912, 986
Msp20I TGGCCA 1 cut(s) 125
MspA1I CMGCKG 1 cut(s) 860
MspI CCGG 2 cut(s) 411, 689
MspR9I CCNGG 4 cut(s) 412, 431, 445, 689
Mva1269I GAATGC 1 cut(s) 554
MvaI CCWGG 2 cut(s) 431, 445
MwoI GCNNNNNNNGC 3 cut(s) 182, 203, 519
NciI CCSGG 2 cut(s) 412, 689
NlaIII CATG 7 cut(s) 124, 140, 145, 368, 585, 724, 796
NlaIV GGNNCC 3 cut(s) 274, 608, 745
PctI GAATGC 1 cut(s) 554
PfeI GAWTC 2 cut(s) 145, 599
PkrI GCNGC 3 cut(s) 184, 359, 377
Ppu21I YACGTR 2 cut(s) 460, 658
PshBI ATTAAT 1 cut(s) 912
Psp6I CCWGG 2 cut(s) 429, 443
PspFI CCCAGC 2 cut(s) 490, 856
PspGI CCWGG 2 cut(s) 429, 443
PspN4I GGNNCC 3 cut(s) 274, 608, 745
PspPI GGNCC 3 cut(s) 320, 447, 686
PstI CTGCAG 1 cut(s) 184
PvuII CAGCTG 1 cut(s) 860
RsaI GTAC 2 cut(s) 110, 314
RsaNI GTAC 2 cut(s) 109, 313
SaqAI TTAA 3 cut(s) 708, 912, 986
SatI GCNGC 3 cut(s) 183, 358, 376
Sau96I GGNCC 3 cut(s) 320, 447, 686
ScrFI CCNGG 4 cut(s) 412, 431, 445, 689
SfaNI GCATC 1 cut(s) 969
SfcI CTRYAG 2 cut(s) 180, 561
SinI GGWCC 1 cut(s) 447
SnaBI TACGTA 1 cut(s) 460
Sse9I AATT 5 cut(s) 89, 633, 906, 938, 983
SsiI CCGC 2 cut(s) 527, 587
SspMI CTAG 4 cut(s) 278, 576, 681, 755
StyD4I CCNGG 4 cut(s) 410, 429, 443, 687
StyI CCWWGG 3 cut(s) 207, 261, 772
TaaI ACNGT 6 cut(s) 232, 312, 478, 562, 880, 994
TaiI ACGT 2 cut(s) 462, 660
TaqI TCGA 3 cut(s) 148, 225, 963
TasI AATT 5 cut(s) 89, 633, 906, 938, 983
TatI WGTACW 2 cut(s) 108, 312
TfiI GAWTC 2 cut(s) 145, 599
Tru1I TTAA 3 cut(s) 708, 912, 986
Tru9I TTAA 3 cut(s) 708, 912, 986
TscAI CASTG 5 cut(s) 315, 407, 483, 565, 934
TseI GCWGC 3 cut(s) 182, 357, 375
TspDTI ATGAA 5 cut(s) 65, 158, 381, 689, 903
TspRI CASTG 5 cut(s) 315, 407, 483, 565, 934
VpaK11BI GGWCC 1 cut(s) 447
VspI ATTAAT 1 cut(s) 912
XspI CTAG 4 cut(s) 278, 576, 681, 755
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.