pycom08g19820
WRKY Family

WRKY transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0011207
Physical Location & Seq
Forward (+)
19780067 .. 19782454
2388 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1710 bp
ATGGGGAATGGAGACAAGGAGAAAGACTTGGATGTTGATCTCTCACTCAAAATTGATGCCAAAGAAGAAAAACAAGAAGAAGGTGTAGGCGGCGAGGCAGAAAAAGAAGAAAAAGATGCTTATCGCGTCAACGATGGCGTTGAAGCAGCAGCAGCAGAAGAAAAGGAGGATGAGCAAGGACCAGGAGTACAGAATAAGAGGCGAAAAACAACAGAAACCGGGGCCGGTGAAGTGGAAGACGATGATGTCTCAGTAGTCGAAACATCGGTGCAGGAGAATAATATGAAGACGGATCAGGAGTTAAGCGTTTTACAAATGGAGATGAGCCGTATGAAAGAAGAAAACAAGGCTTTGAGGAAGGTCGTAGAGCAAACCAAGAAAGATTATTATGATCTTCAAATGAAATTTACGGTTCTCCATCAAAGTAGCCAAAACAAGGATCCTCAAACCTTTCTATCACTTGCTGGTAACGATCCCAACGCAGTTCAAGGACCCAGGAAGATTACCAAGATGTCAGATATTCGCCATAGGTCTCCACTGTCACCGAAACGTGATGATGAGAGTCAACTAGGGTTGTCACTGAGGCTGACCAGCTCTGCTACTACTGCCAGTCAATTAAGGGGAAGAGAAGAAGAAGAAGATCATGATGATAAAGAAGAGATCAATAACAAGGAAGGATTGACGAGGACTACTAGTCTTACATCATCGTCAGTGCAACTGCAAAACAAGTTACAGCGGCCGGAATTGGCCGGAACTACAAGCCACGGTGGCTCCCAACCCAATCGAAAAGCTAGGGTTTCGGTTAGATCACGATGTGAATCGGCCGCAATAAATGATGGGTGCAGTTGGAGGAAATATGGGCAGAAAATCTCAAAAGGAAACCCTTGCCCACGAGCCTATTATCGTTGCACAGTAGTCGCGGGATGCCCAGTCAGAAAACAGGTCCAAAGATGCCAAGAGGACATGTCCATACTTATAACAACTTATGAAGGAACACACAACCATCCTCTTCCAGTTGGTGCGACGGCCATGGGTGCAACAGCCATGGCTTCAACAGCTGCTGCTGCAGCTTCCTTCATGTTACTAGATTCAAGTAATCTTCATTTAGCAGATGGCACACCCTCTACTTACACCCAAGCATCCCTCCCTTACCATCATATGATGAACATGAACCCTTCATCTCATCCCTCGTCAAACTTGCGAAGCGTCGATCCTCATGACCCTTCCAAAGGACTTGTTCTTGACCTCACAAACAACTTCTATGACACCATGGGTGGCAGCTCGTCATCTGTACCACCAGGTTTTAGTACTAATTGGTTGATGAGCAGCACTAGCAGCAGATCTGCAAATTACCCTAGCAGTACTAGTCTCACCAACTGTCTTTTGGCTAACGGCAGTAGATCAACCAGAGTGGCGCTAGGGGACGATCAGAAAGGTAACTGGAGATCAGGGGAAGAAAATATGTCCATGGCTGAAAATGTAAGTGCCATTGCTTCAGATCCCAAATTTAGGGTTGCTGTTGCAGCTGCTATCACATCCCTTATTAACAAAGACAATCATACAACAACTAGTCATCCTGCGCCGAATGCTATTGCCCCAAGAGATCATGGAGACAACAACGGTAGTTCATCTACTGGCAACCTTTGGGCTATTGATGAACATTATCTCGCATCCAACGGTAAGCTCATTCGCAAAAACACCTCAGAATAA

Protein Analysis

570

Amino Acids

61.97

Weight (kDa)

5.76

Isoelectric Point (pI)

51.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WRKY PF03106 278 - 336 8e-25 WRKY DNA -binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011207)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G68150
fragaria_vesca FvH4_5g29820 FvH4_5g29820 FvH4_5g29820
malus_domestica MD08G1227200.v1.1 MD15G1419600.v1.1
prunus_persica Prupe.1G564300_v2.0.a1
pyrus_communis pycom08g19820
rosa_chinensis RchiOBHm_Chr7g0223361
rosa_laevigata RLG00000001986
rosa_multiflora Rmu_sc0005124.1_g000024 Rmu_sc0009107.1_g000007
rosa_roxburghii Rroxscaffold_3G00235870
rosa_rugosa Rorug07G0216200 Rorug07G0216300
rosa_samantha Rh7AG360000 Rh7BG349800 Rh7CG378400 Rh7DG355200
rosa_wichuraiana Rw7G030590

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 977
AasI GACNNNNNNGTC 1 cut(s) 245
AccII CGCG 2 cut(s) 126, 920
AciI CCGC 4 cut(s) 90, 736, 825, 920
AclWI GGATC 6 cut(s) 300, 434, 447, 467, 1205, 1493
AcoI YGGCCR 4 cut(s) 737, 747, 822, 1026
AcsI RAATTY 2 cut(s) 404, 1505
AcuI CTGAAG 1 cut(s) 1479
AdeI CACNNNGTG 1 cut(s) 815
AfaI GTAC 4 cut(s) 189, 1293, 1309, 1363
AfiI CCNNNNNNNGG 3 cut(s) 436, 1229, 1509
AflIII ACRYGT 1 cut(s) 963
AgsI TTSAA 5 cut(s) 143, 398, 488, 1053, 1092
AhdI GACNNNNNGTC 1 cut(s) 693
AhlI ACTAGT 3 cut(s) 692, 1364, 1568
AjnI CCWGG 3 cut(s) 181, 494, 1297
AluBI AGCT 7 cut(s) 594, 791, 1058, 1070, 1281, 1526, 1684
AluI AGCT 7 cut(s) 594, 791, 1058, 1070, 1281, 1526, 1684
Alw26I GTCTC 5 cut(s) 6, 253, 537, 1373, 1605
AlwI GGATC 6 cut(s) 300, 434, 447, 467, 1205, 1493
AlwNI CAGNNNCTG 1 cut(s) 1061
AoxI GGCC 5 cut(s) 222, 737, 747, 822, 1026
ApoI RAATTY 2 cut(s) 404, 1505
AspLEI GCGC 2 cut(s) 1417, 1582
AspS9I GGNCC 4 cut(s) 179, 222, 491, 943
AsuC2I CCSGG 1 cut(s) 220
AsuHPI GGTGA 3 cut(s) 239, 534, 1363
AvaII GGWCC 3 cut(s) 179, 491, 943
BamHI GGATCC 1 cut(s) 439
BauI CACGAG 1 cut(s) 891
BbsI GAAGAC 2 cut(s) 243, 293
BccI CCATC 6 cut(s) 128, 426, 830, 1011, 1106, 1161
BceAI ACGGC 3 cut(s) 312, 1041, 1408
BcgI CGANNNNNNTGC 2 cut(s) 898, 932
BciT130I CCWGG 3 cut(s) 183, 496, 1299
BcnI CCSGG 1 cut(s) 220
BcoDI GTCTC 5 cut(s) 6, 253, 537, 1373, 1605
BcuI ACTAGT 3 cut(s) 692, 1364, 1568
BfaI CTAG 9 cut(s) 569, 693, 792, 1085, 1332, 1356, 1365, 1418, 1569
BfmI CTRYAG 1 cut(s) 1065
BfoI RGCGCY 1 cut(s) 1418
BglI GCCNNNNNGGC 1 cut(s) 768
BglII AGATCT 1 cut(s) 1340
BmcAI AGTACT 2 cut(s) 1309, 1363
Bme1390I CCNGG 4 cut(s) 183, 220, 496, 1299
Bme18I GGWCC 3 cut(s) 179, 491, 943
BmeRI GACNNNNNGTC 1 cut(s) 693
BmgT120I GGNCC 4 cut(s) 179, 222, 491, 943
BmiI GGNNCC 4 cut(s) 223, 441, 493, 772
BmrFI CCNGG 4 cut(s) 183, 220, 496, 1299
BmrI ACTGGG 1 cut(s) 923
BmsI GCATC 6 cut(s) 46, 106, 914, 941, 1148, 1679
BmuI ACTGGG 1 cut(s) 923
BpiI GAAGAC 2 cut(s) 243, 293
BpmI CTGGAG 1 cut(s) 1462
BpuMI CCSGG 1 cut(s) 220
BsaBI GATNNNNATC 3 cut(s) 36, 120, 817
BsaI GGTCTC 1 cut(s) 537
BsaJI CCNNGG 7 cut(s) 219, 494, 763, 1029, 1044, 1269, 1467
BsaXI ACNNNNNCTCC 2 cut(s) 755, 785
Bsc4I CCNNNNNNNGG 3 cut(s) 436, 1229, 1509
Bse118I RCCGGY 1 cut(s) 224
Bse1I ACTGG 5 cut(s) 609, 929, 1013, 1445, 1639
Bse3DI GCAATG 1 cut(s) 1488
Bse8I GATNNNNATC 3 cut(s) 36, 120, 817
BseBI CCWGG 3 cut(s) 183, 496, 1299
BseDI CCNNGG 7 cut(s) 219, 494, 763, 1029, 1044, 1269, 1467
BseGI GGATG 9 cut(s) 37, 175, 929, 1003, 1139, 1183, 1535, 1573, 1670
BseJI GATNNNNATC 3 cut(s) 36, 120, 817
BseLI CCNNNNNNNGG 3 cut(s) 436, 1229, 1509
BseMI GCAATG 1 cut(s) 1488
BseMII CTCAG 2 cut(s) 264, 572
BseNI ACTGG 5 cut(s) 609, 929, 1013, 1445, 1639
BseX3I CGGCCG 2 cut(s) 737, 822
BsgI GTGCAG 2 cut(s) 290, 862
Bsh1236I CGCG 2 cut(s) 126, 920
Bsh1285I CGRYCG 2 cut(s) 740, 825
BshFI GGCC 5 cut(s) 224, 739, 749, 824, 1028
BsiEI CGRYCG 2 cut(s) 740, 825
BsiSI CCGG 4 cut(s) 219, 225, 740, 750
BslFI GGGAC 1 cut(s) 1436
BslI CCNNNNNNNGG 3 cut(s) 436, 1229, 1509
BsmAI GTCTC 5 cut(s) 6, 253, 537, 1373, 1605
BsmFI GGGAC 1 cut(s) 1436
BsmI GAATGC 1 cut(s) 1591
BsnI GGCC 5 cut(s) 224, 739, 749, 824, 1028
Bso31I GGTCTC 1 cut(s) 537
Bsp19I CCATGG 4 cut(s) 1029, 1044, 1269, 1467
BspACI CCGC 4 cut(s) 90, 736, 825, 920
BspANI GGCC 5 cut(s) 224, 739, 749, 824, 1028
BspCNI CTCAG 2 cut(s) 263, 573
BspFNI CGCG 2 cut(s) 126, 920
BspHI TCATGA 2 cut(s) 643, 1216
BspLI GGNNCC 4 cut(s) 223, 441, 493, 772
BspMAI CTGCAG 1 cut(s) 1069
BspPI GGATC 6 cut(s) 300, 434, 447, 467, 1205, 1493
BspTNI GGTCTC 1 cut(s) 537
BsrDI GCAATG 1 cut(s) 1488
BsrFI RCCGGY 1 cut(s) 224
BsrI ACTGG 5 cut(s) 609, 929, 1013, 1445, 1639
BssAI RCCGGY 1 cut(s) 224
BssECI CCNNGG 7 cut(s) 219, 494, 763, 1029, 1044, 1269, 1467
BssSI CACGAG 1 cut(s) 891
BssT1I CCWWGG 4 cut(s) 1029, 1044, 1269, 1467
Bst2BI CACGAG 1 cut(s) 891
Bst2UI CCWGG 3 cut(s) 183, 496, 1299
Bst4CI ACNGT 7 cut(s) 412, 540, 767, 913, 1379, 1622, 1679
Bst6I CTCTTC 3 cut(s) 619, 651, 1014
BstDEI CTNAG 3 cut(s) 250, 581, 1702
BstDSI CCRYGG 5 cut(s) 763, 1029, 1044, 1269, 1467
BstENI CCTNNNNNAGG 1 cut(s) 1227
BstF5I GGATG 9 cut(s) 37, 175, 929, 1003, 1139, 1183, 1535, 1573, 1670
BstFNI CGCG 2 cut(s) 126, 920
BstH2I RGCGCY 1 cut(s) 1418
BstHHI GCGC 2 cut(s) 1417, 1582
BstMAI GTCTC 5 cut(s) 6, 253, 537, 1373, 1605
BstMCI CGRYCG 2 cut(s) 740, 825
BstNI CCWGG 3 cut(s) 183, 496, 1299
BstNSI RCATGY 1 cut(s) 967
BstSCI CCNGG 4 cut(s) 181, 218, 494, 1297
BstSFI CTRYAG 1 cut(s) 1065
BstUI CGCG 2 cut(s) 126, 920
BstV2I GAAGAC 2 cut(s) 243, 293
BstX2I RGATCY 3 cut(s) 439, 1340, 1498
BstYI RGATCY 3 cut(s) 439, 1340, 1498
BstZI CGGCCG 2 cut(s) 737, 822
BsuRI GGCC 5 cut(s) 224, 739, 749, 824, 1028
BtgI CCRYGG 5 cut(s) 763, 1029, 1044, 1269, 1467
BtsCI GGATG 9 cut(s) 37, 175, 929, 1003, 1139, 1183, 1535, 1573, 1670
BtsIMutI CAGTG 3 cut(s) 536, 578, 717
CaiI CAGNNNCTG 1 cut(s) 1061
CciI TCATGA 2 cut(s) 643, 1216
CfoI GCGC 2 cut(s) 1417, 1582
Cfr10I RCCGGY 1 cut(s) 224
Cfr13I GGNCC 4 cut(s) 179, 222, 491, 943
CseI GACGC 2 cut(s) 115, 1195
CsiI ACCWGGT 1 cut(s) 1297
Csp6I GTAC 4 cut(s) 188, 1292, 1308, 1362
CspCI CAANNNNNGTGG 2 cut(s) 1392, 1427
CviQI GTAC 4 cut(s) 188, 1292, 1308, 1362
DdeI CTNAG 3 cut(s) 250, 581, 1702
DraIII CACNNNGTG 1 cut(s) 815
DrdI GACNNNNNNGTC 1 cut(s) 245
DriI GACNNNNNGTC 1 cut(s) 693
DseDI GACNNNNNNGTC 1 cut(s) 245
EaeI YGGCCR 4 cut(s) 737, 747, 822, 1026
EagI CGGCCG 2 cut(s) 737, 822
Eam1104I CTCTTC 3 cut(s) 619, 651, 1014
Eam1105I GACNNNNNGTC 1 cut(s) 693
EarI CTCTTC 3 cut(s) 619, 651, 1014
EclXI CGGCCG 2 cut(s) 737, 822
Eco130I CCWWGG 4 cut(s) 1029, 1044, 1269, 1467
Eco31I GGTCTC 1 cut(s) 537
Eco47I GGWCC 3 cut(s) 179, 491, 943
Eco52I CGGCCG 2 cut(s) 737, 822
Eco57I CTGAAG 1 cut(s) 1479
EcoNI CCTNNNNNAGG 1 cut(s) 1227
EcoO109I RGGNCCY 1 cut(s) 491
EcoRII CCWGG 3 cut(s) 181, 494, 1297
EcoT14I CCWWGG 4 cut(s) 1029, 1044, 1269, 1467
ErhI CCWWGG 4 cut(s) 1029, 1044, 1269, 1467
FaqI GGGAC 1 cut(s) 1436
FauI CCCGC 1 cut(s) 913
FauNDI CATATG 1 cut(s) 1158
FokI GGATG 9 cut(s) 44, 182, 936, 990, 1126, 1170, 1522, 1560, 1657
FspBI CTAG 9 cut(s) 569, 693, 792, 1085, 1332, 1356, 1365, 1418, 1569
GlaI GCGC 2 cut(s) 1416, 1581
GsuI CTGGAG 1 cut(s) 1462
HaeII RGCGCY 1 cut(s) 1418
HaeIII GGCC 5 cut(s) 224, 739, 749, 824, 1028
HapII CCGG 4 cut(s) 219, 225, 740, 750
HgaI GACGC 2 cut(s) 115, 1195
HhaI GCGC 2 cut(s) 1417, 1582
Hin6I GCGC 2 cut(s) 1415, 1580
HinP1I GCGC 2 cut(s) 1415, 1580
HincII GTYRAC 2 cut(s) 130, 566
HindII GTYRAC 2 cut(s) 130, 566
HinfI GANTC 3 cut(s) 562, 818, 1088
HpaII CCGG 4 cut(s) 219, 225, 740, 750
HphI GGTGA 3 cut(s) 239, 534, 1363
Hpy166II GTNNAC 2 cut(s) 130, 566
Hpy188I TCNGA 5 cut(s) 517, 935, 1431, 1498, 1705
Hpy188III TCNNGA 5 cut(s) 296, 644, 810, 1217, 1241
Hpy8I GTNNAC 2 cut(s) 130, 566
Hpy99I CGWCG 2 cut(s) 1027, 1211
HpyAV CCTTC 7 cut(s) 74, 352, 668, 983, 1084, 1185, 1233
HpyCH4III ACNGT 7 cut(s) 412, 540, 767, 913, 1379, 1622, 1679
HpyCH4IV ACGT 1 cut(s) 550
HpyCH4V TGCA 9 cut(s) 271, 715, 721, 843, 909, 1037, 1067, 1346, 1523
HpyF3I CTNAG 3 cut(s) 250, 581, 1702
HpySE526I ACGT 1 cut(s) 550
HspAI GCGC 2 cut(s) 1415, 1580
LmnI GCTCC 1 cut(s) 776
LweI GCATC 6 cut(s) 46, 106, 914, 941, 1148, 1679
MabI ACCWGGT 1 cut(s) 1297
MaeI CTAG 9 cut(s) 569, 693, 792, 1085, 1332, 1356, 1365, 1418, 1569
MaeII ACGT 1 cut(s) 550
MaeIII GTNAC 6 cut(s) 467, 540, 576, 729, 1080, 1436
MflI RGATCY 3 cut(s) 439, 1340, 1498
MluCI AATT 7 cut(s) 51, 404, 614, 743, 1312, 1348, 1505
MlyI GAGTC 1 cut(s) 571
MmeI TCCRAC 2 cut(s) 827, 1698
MseI TTAA 3 cut(s) 302, 617, 1545
MspA1I CMGCKG 3 cut(s) 736, 1058, 1526
MspI CCGG 4 cut(s) 219, 225, 740, 750
MspR9I CCNGG 4 cut(s) 183, 220, 496, 1299
Mva1269I GAATGC 1 cut(s) 1591
MvaI CCWGG 3 cut(s) 183, 496, 1299
MvnI CGCG 2 cut(s) 126, 920
NciI CCSGG 1 cut(s) 220
NcoI CCATGG 4 cut(s) 1029, 1044, 1269, 1467
NdeI CATATG 1 cut(s) 1158
NlaIV GGNNCC 4 cut(s) 223, 441, 493, 772
NmuCI GTSAC 2 cut(s) 540, 576
NspI RCATGY 1 cut(s) 967
PagI TCATGA 2 cut(s) 643, 1216
PciI ACATGT 1 cut(s) 963
PctI GAATGC 1 cut(s) 1591
PfeI GAWTC 2 cut(s) 818, 1088
PleI GAGTC 1 cut(s) 570
PpsI GAGTC 1 cut(s) 570
PpuMI RGGWCCY 1 cut(s) 491
PscI ACATGT 1 cut(s) 963
PsiI TTATAA 1 cut(s) 977
Psp5II RGGWCCY 1 cut(s) 491
Psp6I CCWGG 3 cut(s) 181, 494, 1297
PspGI CCWGG 3 cut(s) 181, 494, 1297
PspN4I GGNNCC 4 cut(s) 223, 441, 493, 772
PspPI GGNCC 4 cut(s) 179, 222, 491, 943
PspPPI RGGWCCY 1 cut(s) 491
PstI CTGCAG 1 cut(s) 1069
PstNI CAGNNNCTG 1 cut(s) 1061
PsuI RGATCY 3 cut(s) 439, 1340, 1498
PvuII CAGCTG 2 cut(s) 1058, 1526
RsaI GTAC 4 cut(s) 189, 1293, 1309, 1363
RsaNI GTAC 4 cut(s) 188, 1292, 1308, 1362
SaqAI TTAA 3 cut(s) 302, 617, 1545
Sau96I GGNCC 4 cut(s) 179, 222, 491, 943
ScaI AGTACT 2 cut(s) 1309, 1363
SchI GAGTC 1 cut(s) 571
ScrFI CCNGG 4 cut(s) 183, 220, 496, 1299
SexAI ACCWGGT 1 cut(s) 1297
SfaNI GCATC 6 cut(s) 46, 106, 914, 941, 1148, 1679
SfcI CTRYAG 1 cut(s) 1065
SinI GGWCC 3 cut(s) 179, 491, 943
SpeI ACTAGT 3 cut(s) 692, 1364, 1568
Sse9I AATT 7 cut(s) 51, 404, 614, 743, 1312, 1348, 1505
SsiI CCGC 4 cut(s) 90, 736, 825, 920
SspMI CTAG 9 cut(s) 569, 693, 792, 1085, 1332, 1356, 1365, 1418, 1569
StyD4I CCNGG 4 cut(s) 181, 218, 494, 1297
StyI CCWWGG 4 cut(s) 1029, 1044, 1269, 1467
TaaI ACNGT 7 cut(s) 412, 540, 767, 913, 1379, 1622, 1679
TaiI ACGT 1 cut(s) 553
TaqI TCGA 3 cut(s) 258, 784, 1209
TasI AATT 7 cut(s) 51, 404, 614, 743, 1312, 1348, 1505
TatI WGTACW 3 cut(s) 187, 1307, 1361
TauI GCSGC 3 cut(s) 93, 739, 827
TfiI GAWTC 2 cut(s) 818, 1088
Tru1I TTAA 3 cut(s) 302, 617, 1545
Tru9I TTAA 3 cut(s) 302, 617, 1545
TscAI CASTG 3 cut(s) 543, 585, 717
TseFI GTSAC 2 cut(s) 540, 576
Tsp45I GTSAC 2 cut(s) 540, 576
TspGWI ACGGA 1 cut(s) 305
TspRI CASTG 3 cut(s) 543, 585, 717
VpaK11BI GGWCC 3 cut(s) 179, 491, 943
XagI CCTNNNNNAGG 1 cut(s) 1227
XapI RAATTY 2 cut(s) 404, 1505
XceI RCATGY 1 cut(s) 967
XcmI CCANNNNNNNNNTGG 1 cut(s) 1381
XspI CTAG 9 cut(s) 569, 693, 792, 1085, 1332, 1356, 1365, 1418, 1569
ZrmI AGTACT 2 cut(s) 1309, 1363
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.