pycom08g19860

Plant protein of unknown function (DUF639)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0003186
Physical Location & Seq
Forward (+)
19812820 .. 19820359
7540 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2490 bp
ATGCTGTCCAAAATCTCAGTTACCCAGTTGAAAACATCACCTGCTGCACCCGTTTCTGGATTTTCATGGCGTGGGAATCGTCCTAGGCTTGGATATTGTGCCGGGAATTCTTCAGAGCGTAACAAGTTGCGGTTCAGGATTGTGGGGCTGTCTCTCGGGGATAGGTGGAAGCTCAATGACTTTGATGCAAATGCAGTACAAGAAAAGTTGAATTCATGGCTGGTAAAGACGCAAAGTTTTTTAAATGAAGTGGCGTCCACGTCTCCATTGGCAAGAACTGATCAGACTAAAAAGCCTGTCACTAGAAATGCTTCAGAGACACAAGATATGGAGGGCATATTTATGGCAGAACAGACAATTAATAGCAGAACACCAAATGGAATTCTCTCTTTAGCTGCTGTTGTATCTATAGAGCAATTTAGCAGGATGAATGGATTGACAGGGCAGAAGATGCAGAAAATATTTAAAGCACTTGTTCCTGAGTCTACATACAATGATGCTCGCAATTTGGTAGAATATTGCTGCTTCAGGTTTCTGTCAAGGGATAATTCTGATATTCATCCTTCCCTCAAGGAACCTTCATTCCAAAGACTTATATTTATAACTATGCTTGCATGGGAGAATCCTTATCGAGAGGATCTTGCTAATGGTTCGGAGAAGGCTTCCTTTCAGAGAAAGCTTGTCAGAGAAGAGGCTTTTGTTCGAATGGCTCCTGCTATTTCTGGTGTGGCTGATAGGTCAACAGCGAATAATCTATTTAAGGCCCTTGCCGGTGATGAACAGGGCATCTCTCTGAGCACATGGTTGACTTATGTTCATGAACTTCTCAAGATTCACGAAGGAAGAAAATCATACCAGACTCGACAATGTTCCAACCTTTCTGAGGAGAGAATTTTATGCATTGGTTCCAGCAGGAAGCGGCCTGTCCTAAAATGGGAGAACAATATGGCATGGCCAGGAAAAGTTACATTAACTGATAAAGCAATTTATTTTGAGGCCGTTGGCCTTGCTGGGCAAAAGGATTCTATAAGATTGGATCTTACAAAACAAGGATTACAGATTAAGAAAGCAAAAGTTGGACCTTTTGGTTCTGGTCTCTTCGACTCTGCCGTCTCTATATCATATGGTCCCAAATCAGAAACATGGGTGCTTGAATTTGTGGACTTAGGTGGAGAAATGAGGCGAGACGTGTGGCATGCTTTTATTAGCGAAATTAATGCTGTACACAAGTTTATCAGTGATTATGGACCGGAGGAAGATGATGAATCCATATTTCATGTTTATGGTGCTCACAAAGGGAAGGAAAGAGCTATGGCCACTGCCATTAATAGTATAGCCAGACTACAGGCTCTTCAATTTATGGGGAAGTTGTTGGATGATCCCACCAAACTTGTTCAGTTTACATATTTACAGTATGCACCATTTGGTGAGGTAGTTTCTCAGACTCTAGCAGTAAATTATTGGGGTGGACCATTGATATCAAAGTATTTAGAGACGGACTACCAGCCAGCTCAAGGAGTGAGGGCTTCTAATGAAATGATTGAAAGCAGCAGCAATCATGTGTTTGACATAGATGGCAGTGTTTACTTGCACAAATGGAAGAGATCTCCATGTTGGGCTTCAAGTGCTTCTGTTACCTTTTGGAAGAACACTTCAACAAGACAGGGGCTAGTTTTAAGTAAAAATCTTGTTGTTGCCGATGTGTCCCTTGTTGAAAGGGCAACAAGAATATGCAAACAAAAATGGCAGGAAGCTGAAACAACTCAAGCCACAATTGATGCTGCAACGCTTAAGGGAATACCAAGTAACATTGATCTATTCAAGGAACTTCTGTTTCCTCTGACCATAACTGTGAGAAATTTCGAAAAACTTAGGCGCTGGGAGGAGCCATATTCGACCATTTCTTTTCTTGCCTTTGCCTATACAGTCATCTTCAGGAATTTGCTGTCATATGTATTCCCAACGGCATTGATAATTTTGGCAGCTGTCATGCTGACATTGAAGGGGCTAAAGGAGCAAGGCCGCCTTGGACGATCATTTGGAAAAATTACTATCCGTGACCAGCCTCCTTCAAATACCATTGAGAAAATTATAGCCGTAAAAGATGCCATGCACGATGTGGAAAGTTATTTACAGAATCTGAATGTCACTCTTCTGAAAATACATACAGTTTTCCTTTCTGGTCAGCCTCAGATAACGACTGAGGTTGCGCTGGTGTTGTTATCTTCTGCAACCATTCTCCTCATTTTCCCCTTCAAATATGTTCTTGCCTTTTTTATCTTTGATCTGTTCACACGGGAGCTTGAGTTCAGGAGGGAAATGGTTAAAAGGTTTATGAAACTCTTGAAGGAGCGTTGGGACAGAGTGCCTGCAGCCCCTGTAGTTGTATTGCCTTTTGGAAGTGAGGAGCCAGCACCAAAACCGAATACAAAGGAAAGCAAGGACCCAGAAAAGTCAGAAAGAAGCCTTGGCAGCAGCAGTTCTGTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF639

Protein Analysis

830

Amino Acids

93.45

Weight (kDa)

9.24

Isoelectric Point (pI)

41.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF639 PF04842 568 - 799 5.3e-84 Plant protein of unknown function (DUF639)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 602
AarI CACCTGC 1 cut(s) 49
AasI GACNNNNNNGTC 1 cut(s) 1109
Acc36I ACCTGC 1 cut(s) 49
AccI GTMKAC 1 cut(s) 485
AciI CCGC 3 cut(s) 130, 919, 2023
AclWI GGATC 3 cut(s) 645, 1044, 1373
AcoI YGGCCR 2 cut(s) 953, 1314
AcsI RAATTY 7 cut(s) 106, 211, 381, 891, 1154, 1858, 1939
AcuI CTGAAG 4 cut(s) 96, 297, 511, 1918
AcyI GRCGYC 1 cut(s) 254
AdeI CACNNNGTG 1 cut(s) 2119
AfaI GTAC 2 cut(s) 198, 1224
AfiI CCNNNNNNNGG 5 cut(s) 56, 89, 883, 934, 1514
AflII CTTAAG 1 cut(s) 1790
AflIII ACRYGT 1 cut(s) 1188
AjiI CACGTC 2 cut(s) 261, 1189
AjnI CCWGG 1 cut(s) 955
AjuI GAANNNNNNNTTGG 2 cut(s) 2451, 2483
AloI GAACNNNNNNTCC 4 cut(s) 567, 599, 2291, 2323
AluBI AGCT 8 cut(s) 172, 395, 679, 1310, 1511, 1754, 1985, 2302
AluI AGCT 8 cut(s) 172, 395, 679, 1310, 1511, 1754, 1985, 2302
Alw21I GWGCWC 2 cut(s) 800, 1291
Alw26I GTCTC 7 cut(s) 156, 267, 311, 1100, 1117, 1179, 1487
AlwI GGATC 3 cut(s) 645, 1044, 1373
AlwNI CAGNNNCTG 2 cut(s) 2140, 2378
Ama87I CYCGRG 1 cut(s) 155
AoxI GGCC 7 cut(s) 762, 920, 953, 996, 1003, 1314, 2020
ApoI RAATTY 7 cut(s) 106, 211, 381, 891, 1154, 1858, 1939
AseI ATTAAT 3 cut(s) 360, 1215, 1326
Asp700I GAANNNNTTC 1 cut(s) 310
AspA2I CCTAGG 1 cut(s) 83
AspLEI GCGC 2 cut(s) 1878, 2212
AspS9I GGNCC 6 cut(s) 763, 1079, 1127, 1247, 1469, 2443
AsuC2I CCSGG 1 cut(s) 103
AsuHPI GGTGA 3 cut(s) 30, 785, 1439
AsuII TTCGAA 2 cut(s) 703, 1863
AvaI CYCGRG 1 cut(s) 155
AvaII GGWCC 5 cut(s) 1079, 1127, 1247, 1469, 2443
AvrII CCTAGG 1 cut(s) 83
BalI TGGCCA 2 cut(s) 955, 1316
Bbv12I GWGCWC 2 cut(s) 800, 1291
BbvI GCAGC 9 cut(s) 31, 382, 509, 1560, 1563, 1768, 1994, 2384, 2484
BccI CCATC 1 cut(s) 1568
BceAI ACGGC 4 cut(s) 983, 1094, 1980, 2081
BcgI CGANNNNNNTGC 2 cut(s) 1199, 1233
BciT130I CCWGG 1 cut(s) 957
BclI TGATCA 1 cut(s) 280
BcnI CCSGG 1 cut(s) 103
BcoDI GTCTC 7 cut(s) 156, 267, 311, 1100, 1117, 1179, 1487
BfaI CTAG 4 cut(s) 84, 303, 1448, 1670
BfmI CTRYAG 4 cut(s) 408, 1343, 2370, 2379
BfoI RGCGCY 1 cut(s) 1879
BfrI CTTAAG 1 cut(s) 1790
BfuAI ACCTGC 1 cut(s) 49
BglII AGATCT 1 cut(s) 1604
BlnI CCTAGG 1 cut(s) 83
Bme1390I CCNGG 2 cut(s) 103, 957
Bme18I GGWCC 5 cut(s) 1079, 1127, 1247, 1469, 2443
BmeT110I CYCGRG 1 cut(s) 155
BmgBI CACGTC 2 cut(s) 261, 1189
BmgT120I GGNCC 6 cut(s) 763, 1079, 1127, 1247, 1469, 2443
BmiI GGNNCC 7 cut(s) 576, 711, 907, 1129, 1887, 2409, 2445
BmrFI CCNGG 2 cut(s) 103, 957
BmrI ACTGGG 1 cut(s) 19
BmsI GCATC 6 cut(s) 175, 441, 487, 795, 1768, 2095
BmuI ACTGGG 1 cut(s) 19
Bpu14I TTCGAA 2 cut(s) 703, 1863
BpuEI CTTGAG 5 cut(s) 554, 812, 1497, 1749, 2324
BpuMI CCSGG 1 cut(s) 103
BsaBI GATNNNNATC 1 cut(s) 558
BsaHI GRCGYC 1 cut(s) 254
BsaI GGTCTC 1 cut(s) 1100
BsaJI CCNNGG 3 cut(s) 83, 2026, 2467
BsaWI WCCGGW 1 cut(s) 1249
BsaXI ACNNNNNCTCC 2 cut(s) 2291, 2321
Bsc4I CCNNNNNNNGG 5 cut(s) 56, 89, 883, 934, 1514
Bse118I RCCGGY 1 cut(s) 770
Bse1I ACTGG 1 cut(s) 25
Bse8I GATNNNNATC 1 cut(s) 558
BseBI CCWGG 1 cut(s) 957
BseDI CCNNGG 3 cut(s) 83, 2026, 2467
BseGI GGATG 3 cut(s) 432, 559, 1381
BseJI GATNNNNATC 1 cut(s) 558
BseLI CCNNNNNNNGG 5 cut(s) 56, 89, 883, 934, 1514
BseMII CTCAG 7 cut(s) 30, 471, 785, 873, 1454, 2193, 2204
BseNI ACTGG 1 cut(s) 25
BseRI GAGGAG 4 cut(s) 899, 1898, 2231, 2420
BseXI GCAGC 9 cut(s) 31, 382, 509, 1560, 1563, 1768, 1994, 2384, 2484
BseYI CCCAGC 2 cut(s) 1010, 1878
BsgI GTGCAG 1 cut(s) 30
BshFI GGCC 7 cut(s) 764, 922, 955, 998, 1005, 1316, 2022
BsiHKAI GWGCWC 2 cut(s) 800, 1291
BsiHKCI CYCGRG 1 cut(s) 155
BsiSI CCGG 3 cut(s) 102, 771, 1250
BslFI GGGAC 3 cut(s) 1113, 1690, 2372
BslI CCNNNNNNNGG 5 cut(s) 56, 89, 883, 934, 1514
BsmAI GTCTC 7 cut(s) 156, 267, 311, 1100, 1117, 1179, 1487
BsmBI CGTCTC 4 cut(s) 267, 1117, 1179, 1487
BsmFI GGGAC 3 cut(s) 1113, 1690, 2372
BsnI GGCC 7 cut(s) 764, 922, 955, 998, 1005, 1316, 2022
Bso31I GGTCTC 1 cut(s) 1100
BsoBI CYCGRG 1 cut(s) 155
Bsp119I TTCGAA 2 cut(s) 703, 1863
Bsp1286I GDGCHC 2 cut(s) 800, 1291
Bsp1407I TGTACA 1 cut(s) 1222
Bsp143I GATC 8 cut(s) 280, 637, 1036, 1378, 1604, 1813, 2033, 2284
BspACI CCGC 3 cut(s) 130, 919, 2023
BspANI GGCC 7 cut(s) 764, 922, 955, 998, 1005, 1316, 2022
BspCNI CTCAG 7 cut(s) 29, 472, 786, 874, 1453, 2194, 2203
BspHI TCATGA 1 cut(s) 817
BspLI GGNNCC 7 cut(s) 576, 711, 907, 1129, 1887, 2409, 2445
BspMAI CTGCAG 1 cut(s) 2374
BspMI ACCTGC 1 cut(s) 49
BspPI GGATC 3 cut(s) 645, 1044, 1373
BspQI GCTCTTC 1 cut(s) 1356
BspT104I TTCGAA 2 cut(s) 703, 1863
BspTI CTTAAG 1 cut(s) 1790
BspTNI GGTCTC 1 cut(s) 1100
BsrFI RCCGGY 1 cut(s) 770
BsrGI TGTACA 1 cut(s) 1222
BsrI ACTGG 1 cut(s) 25
BssAI RCCGGY 1 cut(s) 770
BssECI CCNNGG 3 cut(s) 83, 2026, 2467
BssMI GATC 8 cut(s) 280, 637, 1036, 1378, 1604, 1813, 2033, 2284
BssNI GRCGYC 1 cut(s) 254
BssT1I CCWWGG 3 cut(s) 83, 2026, 2467
Bst2UI CCWGG 1 cut(s) 957
Bst4CI ACNGT 4 cut(s) 1413, 1852, 1927, 2170
Bst6I CTCTTC 5 cut(s) 684, 1103, 1356, 1595, 2157
BstACI GRCGYC 1 cut(s) 254
BstAFI CTTAAG 1 cut(s) 1790
BstAPI GCANNNNNTGC 1 cut(s) 451
BstAUI TGTACA 1 cut(s) 1222
BstBI TTCGAA 2 cut(s) 703, 1863
BstC8I GCNNGC 6 cut(s) 502, 612, 1197, 1509, 2370, 2412
BstDEI CTNAG 9 cut(s) 16, 480, 794, 882, 1165, 1440, 1871, 2190, 2202
BstENI CCTNNNNNAGG 1 cut(s) 881
BstF5I GGATG 3 cut(s) 432, 559, 1381
BstH2I RGCGCY 1 cut(s) 1879
BstHHI GCGC 2 cut(s) 1878, 2212
BstKTI GATC 8 cut(s) 283, 640, 1039, 1381, 1607, 1816, 2036, 2287
BstMAI GTCTC 7 cut(s) 156, 267, 311, 1100, 1117, 1179, 1487
BstMBI GATC 8 cut(s) 280, 637, 1036, 1378, 1604, 1813, 2033, 2284
BstMWI GCNNNNNNNGC 4 cut(s) 451, 1625, 2014, 2472
BstNI CCWGG 1 cut(s) 957
BstNSI RCATGY 1 cut(s) 1199
BstSCI CCNGG 2 cut(s) 101, 955
BstSFI CTRYAG 4 cut(s) 408, 1343, 2370, 2379
BstV1I GCAGC 9 cut(s) 31, 382, 509, 1560, 1563, 1768, 1994, 2384, 2484
BstX2I RGATCY 3 cut(s) 637, 1036, 1604
BstYI RGATCY 3 cut(s) 637, 1036, 1604
BsuRI GGCC 7 cut(s) 764, 922, 955, 998, 1005, 1316, 2022
BtrI CACGTC 2 cut(s) 261, 1189
BtsCI GGATG 3 cut(s) 432, 559, 1381
BtsI GCAGTG 2 cut(s) 1317, 1585
BtsIMutI CAGTG 3 cut(s) 1243, 1317, 1585
BveI ACCTGC 1 cut(s) 49
Cac8I GCNNGC 6 cut(s) 502, 612, 1197, 1509, 2370, 2412
CaiI CAGNNNCTG 2 cut(s) 2140, 2378
CciI TCATGA 1 cut(s) 817
CfoI GCGC 2 cut(s) 1878, 2212
Cfr10I RCCGGY 1 cut(s) 770
Cfr13I GGNCC 6 cut(s) 763, 1079, 1127, 1247, 1469, 2443
CseI GACGC 2 cut(s) 238, 243
Csp6I GTAC 2 cut(s) 197, 1223
CspCI CAANNNNNGTGG 2 cut(s) 1372, 1407
CviQI GTAC 2 cut(s) 197, 1223
DdeI CTNAG 9 cut(s) 16, 480, 794, 882, 1165, 1440, 1871, 2190, 2202
DpnI GATC 8 cut(s) 282, 639, 1038, 1380, 1606, 1815, 2035, 2286
DpnII GATC 8 cut(s) 280, 637, 1036, 1378, 1604, 1813, 2033, 2284
DraI TTTAAA 2 cut(s) 243, 466
DraIII CACNNNGTG 1 cut(s) 2119
DrdI GACNNNNNNGTC 1 cut(s) 1109
DseDI GACNNNNNNGTC 1 cut(s) 1109
EaeI YGGCCR 2 cut(s) 953, 1314
Eam1104I CTCTTC 5 cut(s) 684, 1103, 1356, 1595, 2157
EarI CTCTTC 5 cut(s) 684, 1103, 1356, 1595, 2157
Eco130I CCWWGG 3 cut(s) 83, 2026, 2467
Eco31I GGTCTC 1 cut(s) 1100
Eco32I GATATC 1 cut(s) 1479
Eco47I GGWCC 5 cut(s) 1079, 1127, 1247, 1469, 2443
Eco57I CTGAAG 4 cut(s) 96, 297, 511, 1918
Eco88I CYCGRG 1 cut(s) 155
EcoNI CCTNNNNNAGG 1 cut(s) 881
EcoO109I RGGNCCY 2 cut(s) 763, 2443
EcoRI GAATTC 3 cut(s) 106, 211, 381
EcoRII CCWGG 1 cut(s) 955
EcoRV GATATC 1 cut(s) 1479
EcoT14I CCWWGG 3 cut(s) 83, 2026, 2467
EcoT22I ATGCAT 1 cut(s) 902
ErhI CCWWGG 3 cut(s) 83, 2026, 2467
Esp3I CGTCTC 4 cut(s) 267, 1117, 1179, 1487
FalI AAGNNNNNCTT 4 cut(s) 650, 682, 2010, 2042
FaqI GGGAC 3 cut(s) 1113, 1690, 2372
FauNDI CATATG 2 cut(s) 1123, 1951
FbaI TGATCA 1 cut(s) 280
FblI GTMKAC 1 cut(s) 485
FokI GGATG 3 cut(s) 439, 546, 1388
FspBI CTAG 4 cut(s) 84, 303, 1448, 1670
GlaI GCGC 2 cut(s) 1877, 2211
GsaI CCCAGC 2 cut(s) 1014, 1882
HaeII RGCGCY 1 cut(s) 1879
HaeIII GGCC 7 cut(s) 764, 922, 955, 998, 1005, 1316, 2022
HapII CCGG 3 cut(s) 102, 771, 1250
HgaI GACGC 2 cut(s) 238, 243
HhaI GCGC 2 cut(s) 1878, 2212
Hin1I GRCGYC 1 cut(s) 254
Hin6I GCGC 2 cut(s) 1876, 2210
HinP1I GCGC 2 cut(s) 1876, 2210
HincII GTYRAC 2 cut(s) 741, 807
HindII GTYRAC 2 cut(s) 741, 807
HindIII AAGCTT 1 cut(s) 677
HpaII CCGG 3 cut(s) 102, 771, 1250
HphI GGTGA 3 cut(s) 30, 785, 1439
HpyAV CCTTC 9 cut(s) 573, 588, 652, 833, 1294, 1996, 2079, 2263, 2341
HpyCH4III ACNGT 4 cut(s) 1413, 1852, 1927, 2170
HpyCH4IV ACGT 2 cut(s) 260, 1188
HpyF10VI GCNNNNNNNGC 4 cut(s) 451, 1625, 2014, 2472
HpyF3I CTNAG 9 cut(s) 16, 480, 794, 882, 1165, 1440, 1871, 2190, 2202
HpySE526I ACGT 2 cut(s) 260, 1188
Hsp92I GRCGYC 1 cut(s) 254
HspAI GCGC 2 cut(s) 1876, 2210
Ksp22I TGATCA 1 cut(s) 280
Kzo9I GATC 8 cut(s) 280, 637, 1036, 1378, 1604, 1813, 2033, 2284
LguI GCTCTTC 1 cut(s) 1356
LmnI GCTCC 6 cut(s) 715, 1885, 2014, 2299, 2350, 2407
Lsp1109I GCAGC 9 cut(s) 31, 382, 509, 1560, 1563, 1768, 1994, 2384, 2484
LweI GCATC 6 cut(s) 175, 441, 487, 795, 1768, 2095
MaeI CTAG 4 cut(s) 84, 303, 1448, 1670
MaeII ACGT 2 cut(s) 260, 1188
MaeIII GTNAC 8 cut(s) 19, 119, 298, 964, 1633, 1805, 2057, 2146
MalI GATC 8 cut(s) 282, 639, 1038, 1380, 1606, 1815, 2035, 2286
MboI GATC 8 cut(s) 280, 637, 1036, 1378, 1604, 1813, 2033, 2284
MfeI CAATTG 1 cut(s) 1773
MflI RGATCY 3 cut(s) 637, 1036, 1604
MhlI GDGCHC 2 cut(s) 800, 1291
MlsI TGGCCA 2 cut(s) 955, 1316
MluNI TGGCCA 2 cut(s) 955, 1316
MlyI GAGTC 4 cut(s) 491, 853, 1097, 1438
MmeI TCCRAC 3 cut(s) 897, 1057, 1353
Mox20I TGGCCA 2 cut(s) 955, 1316
Mph1103I ATGCAT 1 cut(s) 902
MroXI GAANNNNTTC 1 cut(s) 310
MscI TGGCCA 2 cut(s) 955, 1316
MslI CAYNNNNRTG 3 cut(s) 341, 1281, 1850
Msp20I TGGCCA 2 cut(s) 955, 1316
MspA1I CMGCKG 1 cut(s) 1985
MspCI CTTAAG 1 cut(s) 1790
MspI CCGG 3 cut(s) 102, 771, 1250
MspR9I CCNGG 2 cut(s) 103, 957
MunI CAATTG 1 cut(s) 1773
MvaI CCWGG 1 cut(s) 957
MwoI GCNNNNNNNGC 4 cut(s) 451, 1625, 2014, 2472
NciI CCSGG 1 cut(s) 103
NdeI CATATG 2 cut(s) 1123, 1951
NdeII GATC 8 cut(s) 280, 637, 1036, 1378, 1604, 1813, 2033, 2284
NlaIV GGNNCC 7 cut(s) 576, 711, 907, 1129, 1887, 2409, 2445
NmuCI GTSAC 3 cut(s) 298, 2057, 2146
NsiI ATGCAT 1 cut(s) 902
NspI RCATGY 1 cut(s) 1199
NspV TTCGAA 2 cut(s) 703, 1863
PaeI GCATGC 1 cut(s) 1199
PagI TCATGA 1 cut(s) 817
PaqCI CACCTGC 1 cut(s) 49
PciSI GCTCTTC 1 cut(s) 1356
PcsI WCGNNNNNNNCGW 1 cut(s) 1107
PdmI GAANNNNTTC 1 cut(s) 310
PfeI GAWTC 6 cut(s) 76, 622, 832, 1022, 1265, 2137
PleI GAGTC 4 cut(s) 490, 853, 1097, 1438
PpsI GAGTC 4 cut(s) 490, 853, 1097, 1438
PpuMI RGGWCCY 1 cut(s) 2443
PshBI ATTAAT 3 cut(s) 360, 1215, 1326
PsiI TTATAA 1 cut(s) 602
Psp5II RGGWCCY 1 cut(s) 2443
Psp6I CCWGG 1 cut(s) 955
PspFI CCCAGC 2 cut(s) 1010, 1878
PspGI CCWGG 1 cut(s) 955
PspN4I GGNNCC 7 cut(s) 576, 711, 907, 1129, 1887, 2409, 2445
PspPI GGNCC 6 cut(s) 763, 1079, 1127, 1247, 1469, 2443
PspPPI RGGWCCY 1 cut(s) 2443
PstI CTGCAG 1 cut(s) 2374
PstNI CAGNNNCTG 2 cut(s) 2140, 2378
PsuI RGATCY 3 cut(s) 637, 1036, 1604
PvuII CAGCTG 1 cut(s) 1985
RsaI GTAC 2 cut(s) 198, 1224
RsaNI GTAC 2 cut(s) 197, 1223
RseI CAYNNNNRTG 3 cut(s) 341, 1281, 1850
SapI GCTCTTC 1 cut(s) 1356
Sau3AI GATC 8 cut(s) 280, 637, 1036, 1378, 1604, 1813, 2033, 2284
Sau96I GGNCC 6 cut(s) 763, 1079, 1127, 1247, 1469, 2443
SchI GAGTC 4 cut(s) 491, 853, 1097, 1438
ScrFI CCNGG 2 cut(s) 103, 957
SduI GDGCHC 2 cut(s) 800, 1291
SfaNI GCATC 6 cut(s) 175, 441, 487, 795, 1768, 2095
SfcI CTRYAG 4 cut(s) 408, 1343, 2370, 2379
SfuI TTCGAA 2 cut(s) 703, 1863
SinI GGWCC 5 cut(s) 1079, 1127, 1247, 1469, 2443
SmiMI CAYNNNNRTG 3 cut(s) 341, 1281, 1850
SmlI CTYRAG 6 cut(s) 569, 827, 1512, 1764, 1790, 2303
SmoI CTYRAG 6 cut(s) 569, 827, 1512, 1764, 1790, 2303
SphI GCATGC 1 cut(s) 1199
SsiI CCGC 3 cut(s) 130, 919, 2023
SspI AATATT 2 cut(s) 462, 518
SspMI CTAG 4 cut(s) 84, 303, 1448, 1670
StyD4I CCNGG 2 cut(s) 101, 955
StyI CCWWGG 3 cut(s) 83, 2026, 2467
TaaI ACNGT 4 cut(s) 1413, 1852, 1927, 2170
TaiI ACGT 2 cut(s) 263, 1191
TaqI TCGA 6 cut(s) 631, 703, 862, 1101, 1863, 1895
TatI WGTACW 2 cut(s) 196, 1222
TauI GCSGC 2 cut(s) 922, 2025
TfiI GAWTC 6 cut(s) 76, 622, 832, 1022, 1265, 2137
TscAI CASTG 3 cut(s) 1243, 1324, 1585
TseFI GTSAC 3 cut(s) 298, 2057, 2146
Tsp45I GTSAC 3 cut(s) 298, 2057, 2146
TspGWI ACGGA 2 cut(s) 1511, 2045
TspRI CASTG 3 cut(s) 1243, 1324, 1585
Vha464I CTTAAG 1 cut(s) 1790
VpaK11BI GGWCC 5 cut(s) 1079, 1127, 1247, 1469, 2443
VspI ATTAAT 3 cut(s) 360, 1215, 1326
XagI CCTNNNNNAGG 1 cut(s) 881
XapI RAATTY 7 cut(s) 106, 211, 381, 891, 1154, 1858, 1939
XceI RCATGY 1 cut(s) 1199
XcmI CCANNNNNNNNNTGG 2 cut(s) 265, 2116
XmaJI CCTAGG 1 cut(s) 83
XmiI GTMKAC 1 cut(s) 485
XmnI GAANNNNTTC 1 cut(s) 310
XspI CTAG 4 cut(s) 84, 303, 1448, 1670
Zsp2I ATGCAT 1 cut(s) 902
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.