Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr8

Orthogroup ID

OG0003447

Physical Location & Seq

Genomic Coordinates Forward (+)

20228103 .. 20235077

Gene Structure 6975 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2610 bp

ATGGAGTTTTGGAAGTTTAGTCCCCACAAGAATCTTGATCATCGGTTTCAGCAACCAGTAGCAGCTGCAGAAGCTGCCTCTTACAACATGAAGAAGGTCTGCAGCTGGAATAATCATACGCATAAGCAGCACCAGCAGCAGCTGCAGCAGCATAAAAATAGTCATGAAAAAGTGAACCCTTCGTCGTCGTCGTCTTCGTCGGAGCTTGATTTAAGAAACATTTCAAAGCTTATCCTTCCTCCCTTGGGTGCTTCGAGTTACAACCAAACTCCTATCCAGTCAAATGGATGGATCGTCTCGCCCATGGATTCAAGATACAGGTGTTGGGAAACATTCATGGTGGTTCTGGTGGCATACTCGGCGTGGGTTTACCCTTTTGAGGTTGCATTTCTTAACACGTCCAAAAGCAGGCCACTATACATTGCAGACAACGTCGTCGATCTATTTTTTGCCGCAGACATTATCTTGACATTCTTCGTCGCATATGTTGATCCAAGAACTCAGTTACTTGTTCATGAATCTAAAAAAATCGCCATGAGGTACCTTTCGACTTGGTTTTTGATGGATTTGGCATCAACACTGCCATTTGAGGCACTTTTTTTAGCACTGGGAAAAAACAAATTGAGTGCCTCTTACTCTCTCTTGGGATTGCTCAGATTCTGGCGACTAAGACGGGCAAAGCAACTCTTCACAAGACTAGAGAAGGATATCAGGTTTAGCTATTTTTGGGTCAGATGTGCAAGGCTTTTATCTGTGACAGTGTTTTCAGTACACTGTGCTGGATGCCTGTACTACCTGCTAGCAGATCGATATCCACACCGAGGGAAGACGTGGATTGGAACTGTGTTTCCAAATTTCAGAGAAACATGCCTCTGGATCCGATACATCTCAGCCGTATACTGGTCTATGACCACCATGAGCACCGTCGGCTACGGCGATCTCCACGCAGTTAACACCGTCGAAATGATCTTCATTATTTTCTACATGCTCTTCAACCTTGGCCTCACTGCTTACTTGATTGGCAACATGACCAACTTGGTTGTTGAAGGCACTCGTCGTACCATGGAATTCAGAAGTAGCATTGAAGCAGCATCAAGTTTTGTGTGCCGGAATCGGTTGCCTCAACGGCTGAGGGACCAAATATTGGCATATATTTGCTTGAGATTTAAGGCGGAGACCTTGAACCAGCAACAGATGCTTGAACAGTTTCCCAAATCCATTTGCAAAAGCATATGCCAACATCTGTTTTTACCAATTGTGGAAAAAGTGTATCTTTTCAAAGGTGTCTCAAGGGAAACACTTCTGCATCTGGTTGTGAATATGAAGGCAGAGTATACACCTCCCAGAGAAGATGTTCTGCTGCAAAACGAAGCGCCGGAGGATGTTTATATCATTGTTTCTGGAGAAGTGGAAATAATTGATTGTGAGATGGAGAGAGAACGAGTTGTTGGGACTTTGCAATCTGGAGACATGTTTGGAGAAGTTATTGCATTTTGTGGTAGAGAAGTTGGACCTCAAAGCTTTACGTACCGAACCAAAACACTGTCACAGCTCTTGAGAATAAAAACCACTGCCCTAGTAGAAGCAATGCAGACCAAACCACATGACAAAGAACAAATGGTTAAAAACTTCAACCAGCATCACAAAAAGCTTAGGGATCTGAGAGTCGGAGAAATACTGGTTGAGAGTGGAGAGGACGAAGGTGGTGACCCAAAGGCGGCATTCAACGTGTTGACTGCTGCCAGCAAAGGCGATGCTACTCTTCTTGATGAGCTTCTCAAGGCAAAGTTGGATCCCGATATTGGAGACTTCAAGGGAAGAACTCCCCTGCACATAGCAGCATCAAAAGGCCACGACGAATGTGTTTTGGTACTCCTGAAGTATGCTTGTAACGTACATGTAAGAGACACAAATGGTAACACAGCGTTATGGGATGCCATTGCATCTAAGCACCATTCCATATTCCAAAGTCTCTACTATTGCGCTGCCATATCTGATCCATACACCGCTGGCGATCTTCTATGCACTGCCGCCAAGAGAAAGGATCTAACCGTAATGGAGGAACTCTTGAAGCAGGGATTAAATGTCGACTCAAAGAATCATCTTGGGATAACAGCCGTACAAATAGCCATGGAAGAAAACAATGCAGACATGGTAAATTTGCTAGTGATGAATGGTGCAGATGTTACCAATGCACAGAACTGTTTATTTCCTTCACAAAGTTTGACCGAAATGCTACAAAAACGAGAGGTTGGACACCGGATTACAGTTCCAGACAGTACAATAAATGAAGTGCCTTTGATAAAGAATGGAGGAGAGCGTAATTCTGGGAGAGAATCCTATGGAGTAAATTGTCCAAGAGTGAGCATATACAGAGGCCATCCTATGGTTAGGAAAACAACATGTTGCAGGGAAGCTGGTAGAGTAATTAGATTGCCTCGTTCCCTAGAGGAACTCCGAAACATTGCAGGTGTAAAGTTTGGATTTGATTCAGGAAATACATTGGTAACAGATGAAGGAGGAGCGGAAATTGACTCAATAGAAGTGATACGAGATAATGACAAACTTTTCCTGGTTGAACTTGAAGACCCAAAGTCTGTCAAACCCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0005215 GO:0005216 GO:0005244 GO:0005249 GO:0005261 GO:0005267 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0005886 GO:0005887 GO:0005911 GO:0006810 GO:0006811 GO:0006812 GO:0006813 GO:0008150 GO:0008324 GO:0009506 GO:0009719 GO:0009725 GO:0009737 GO:0009987 GO:0010033 GO:0012505 GO:0015075 GO:0015077 GO:0015079 GO:0015267 GO:0015318 GO:0015672 GO:0016020 GO:0016021 GO:0022803 GO:0022832 GO:0022836 GO:0022838 GO:0022839 GO:0022843 GO:0022857 GO:0022890 GO:0030001 GO:0030054 GO:0031224 GO:0031226 GO:0031984 GO:0033993 GO:0034220 GO:0042175 GO:0042221 GO:0042391 GO:0042802 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044459 GO:0044464 GO:0046873 GO:0050896 GO:0051179 GO:0051234 GO:0055044 GO:0055085 GO:0065007 GO:0065008 GO:0071804 GO:0071805 GO:0071944 GO:0097305 GO:0098655 GO:0098660 GO:0098662 GO:0098827 GO:1901700

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Ank_2 Ion_trans KHA cNMP_binding

Protein Analysis

870

Amino Acids

98.69

Weight (kDa)

8.23

Isoelectric Point (pI)

34.67

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Ion_trans	PF00520	106 - 354	8.7e-44	Ion transport protein
Ion_trans_2	PF07885	280 - 348	5.4e-13	Ion channel
cNMP_binding	PF00027	447 - 532	6.4e-12	Cyclic nucleotide-binding domain
Ank_2	PF12796	579 - 660	2.3e-14	Ankyrin repeats (3 copies)
Ank_5	PF13857	592 - 643	5.1e-10	Ankyrin repeats (many copies)
Ank	PF00023	606 - 636	1.3e-07	Ankyrin repeat
KHA	PF11834	787 - 858	1.2e-21	KHA, dimerisation domain of potassium ion channel

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0003447)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G22200 AT4G22200
fragaria_vesca	FvH4_5g28980 FvH4_5g28980 FvH4_5g28980 FvH4_5g28980 FvH4_5g28980 FvH4_5g28980 FvH4_5g28980 FvH4_5g28980 FvH4_5g28980 FvH4_5g28980 FvH4_5g28980 FvH4_5g28980 FvH4_5g28980 FvH4_5g28980
malus_domestica	MD08G1236000.v1.1
prunus_persica	Prupe.1G572200_v2.0.a1 Prupe.1G572200_v2.0.a1 Prupe.1G572200_v2.0.a1
pyrus_communis	pycom08g20420
rosa_chinensis	RchiOBHm_Chr7g0221301 RchiOBHm_Chr7g0222541
rosa_laevigata	RLG00000002163
rosa_multiflora	Rmu_co8307231.1_g000001 Rmu_sc0000324.1_g000021
rosa_roxburghii	Rroxscaffold_3G00238430
rosa_rugosa	Rorug07G0202000
rosa_samantha	Rh6BG194600 Rh7AG341200 Rh7AG352200 Rh7BG332200 Rh7CG359100 Rh7CG369500 Rh7DG339700
rosa_wichuraiana	Rw7G029020

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	2459
AasI	GACNNNNNNGTC	1 cut(s)	434
Acc36I	ACCTGC	2 cut(s)	804, 2459
Acc65I	GGTACC	1 cut(s)	540
AccB1I	GGYRCC	1 cut(s)	540
AccB7I	CCANNNNNTGG	2 cut(s)	1144, 2386
AccBSI	CCGCTC	1 cut(s)	2525
AccI	GTMKAC	3 cut(s)	897, 1334, 2088
AciI	CCGC	6 cut(s)	453, 1172, 1718, 2007, 2031, 2525
AclWI	GGATC	9 cut(s)	299, 485, 871, 884, 1665, 1787, 1800, 1991, 2052
AcsI	RAATTY	3 cut(s)	853, 1067, 2158
AcuI	CTGAAG	1 cut(s)	1898
AfaI	GTAC	9 cut(s)	542, 771, 791, 1060, 1529, 1872, 1896, 2121, 2281
AfiI	CCNNNNNNNGG	9 cut(s)	245, 379, 408, 821, 900, 1144, 1717, 1802, 2386
AflIII	ACRYGT	5 cut(s)	396, 1470, 1728, 1897, 2402
AhdI	GACNNNNNGTC	1 cut(s)	2593
AjiI	CACGTC	2 cut(s)	399, 831
AjnI	CCWGG	1 cut(s)	2571
AjuI	GAANNNNNNNTTGG	2 cut(s)	1431, 1463
AloI	GAACNNNNNNTCC	2 cut(s)	832, 864
Alw21I	GWGCWC	1 cut(s)	923
Alw26I	GTCTC	7 cut(s)	301, 1169, 1291, 1461, 1800, 1899, 1976
AlwI	GGATC	9 cut(s)	299, 485, 871, 884, 1665, 1787, 1800, 1991, 2052
AlwNI	CAGNNNCTG	3 cut(s)	74, 142, 660
AoxI	GGCC	4 cut(s)	410, 1000, 1849, 2377
ApoI	RAATTY	3 cut(s)	853, 1067, 2158
ArsI	GACNNNNNNTTYG	2 cut(s)	541, 573
Asp700I	GAANNNNTTC	4 cut(s)	220, 1206, 1299, 1353
Asp718I	GGTACC	1 cut(s)	540
AspLEI	GCGC	2 cut(s)	1375, 1985
AspS9I	GGNCC	2 cut(s)	1135, 1511
AsuHPI	GGTGA	1 cut(s)	1718
AsuNHI	GCTAGC	1 cut(s)	799
AvaII	GGWCC	2 cut(s)	1135, 1511
BaeI	ACNNNNGTAYC	2 cut(s)	307, 340
BamHI	GGATCC	2 cut(s)	876, 1792
BanI	GGYRCC	1 cut(s)	540
BbsI	GAAGAC	3 cut(s)	186, 833, 2592
Bbv12I	GWGCWC	1 cut(s)	923
BbvCI	CCTCAGC	1 cut(s)	1130
BccI	CCATC	4 cut(s)	282, 556, 1423, 2388
BceAI	ACGGC	4 cut(s)	878, 949, 1142, 2102
BciT130I	CCWGG	1 cut(s)	2573
BclI	TGATCA	1 cut(s)	37
BcoDI	GTCTC	7 cut(s)	301, 1169, 1291, 1461, 1800, 1899, 1976
BfaI	CTAG	5 cut(s)	698, 800, 1577, 2165, 2447
BfmI	CTRYAG	3 cut(s)	66, 100, 143
BfoI	RGCGCY	1 cut(s)	1376
BfuAI	ACCTGC	2 cut(s)	804, 2459
BglI	GCCNNNNNGGC	1 cut(s)	1126
Bme1390I	CCNGG	1 cut(s)	2573
Bme18I	GGWCC	2 cut(s)	1135, 1511
BmeRI	GACNNNNNGTC	1 cut(s)	2593
BmgBI	CACGTC	2 cut(s)	399, 831
BmgT120I	GGNCC	2 cut(s)	1135, 1511
BmiI	GGNNCC	4 cut(s)	542, 878, 1136, 1794
BmrFI	CCNGG	1 cut(s)	2573
BmrI	ACTGGG	1 cut(s)	617
BmtI	GCTAGC	1 cut(s)	803
BmuI	ACTGGG	1 cut(s)	617
BpiI	GAAGAC	3 cut(s)	186, 833, 2592
BpmI	CTGGAG	2 cut(s)	1422, 1485
Bpu10I	CCTNAGC	2 cut(s)	1130, 1652
BpuEI	CTTGAG	4 cut(s)	1180, 1273, 1576, 1763
Bsa29I	ATCGAT	1 cut(s)	808
BsaAI	YACGTR	1 cut(s)	1527
BsaBI	GATNNNNATC	1 cut(s)	810
BsaI	GGTCTC	1 cut(s)	1169
BsaJI	CCNNGG	6 cut(s)	243, 303, 820, 997, 1062, 2130
BsaWI	WCCGGW	1 cut(s)	2259
Bsc4I	CCNNNNNNNGG	9 cut(s)	245, 379, 408, 821, 900, 1144, 1717, 1802, 2386
Bse1I	ACTGG	5 cut(s)	56, 277, 612, 905, 1683
Bse3DI	GCAATG	4 cut(s)	420, 1593, 1938, 2463
Bse8I	GATNNNNATC	1 cut(s)	810
BseBI	CCWGG	1 cut(s)	2573
BseCI	ATCGAT	1 cut(s)	808
BseDI	CCNNGG	6 cut(s)	243, 303, 820, 997, 1062, 2130
BseGI	GGATG	5 cut(s)	293, 788, 1387, 1939, 2380
BseJI	GATNNNNATC	1 cut(s)	810
BseLI	CCNNNNNNNGG	9 cut(s)	245, 379, 408, 821, 900, 1144, 1717, 1802, 2386
BseMI	GCAATG	4 cut(s)	420, 1593, 1938, 2463
BseMII	CTCAG	5 cut(s)	515, 667, 903, 1121, 1652
BseNI	ACTGG	5 cut(s)	56, 277, 612, 905, 1683
BseRI	GAGGAG	2 cut(s)	2328, 2535
BsgI	GTGCAG	2 cut(s)	1814, 2199
BshFI	GGCC	4 cut(s)	412, 1002, 1851, 2379
BshNI	GGYRCC	1 cut(s)	540
BshVI	ATCGAT	1 cut(s)	808
BsiHKAI	GWGCWC	1 cut(s)	923
BsiSI	CCGG	3 cut(s)	1108, 1376, 2260
BslFI	GGGAC	3 cut(s)	6, 1148, 1465
BslI	CCNNNNNNNGG	9 cut(s)	245, 379, 408, 821, 900, 1144, 1717, 1802, 2386
BsmAI	GTCTC	7 cut(s)	301, 1169, 1291, 1461, 1800, 1899, 1976
BsmBI	CGTCTC	1 cut(s)	301
BsmFI	GGGAC	3 cut(s)	6, 1148, 1465
BsmI	GAATGC	1 cut(s)	1721
BsnI	GGCC	4 cut(s)	412, 1002, 1851, 2379
Bso31I	GGTCTC	1 cut(s)	1169
Bsp1286I	GDGCHC	1 cut(s)	923
Bsp19I	CCATGG	3 cut(s)	303, 1062, 2130
BspACI	CCGC	6 cut(s)	453, 1172, 1718, 2007, 2031, 2525
BspANI	GGCC	4 cut(s)	412, 1002, 1851, 2379
BspCNI	CTCAG	5 cut(s)	514, 666, 902, 1122, 1653
BspDI	ATCGAT	1 cut(s)	808
BspHI	TCATGA	2 cut(s)	163, 514
BspLI	GGNNCC	4 cut(s)	542, 878, 1136, 1794
BspMAI	CTGCAG	3 cut(s)	70, 104, 147
BspMI	ACCTGC	2 cut(s)	804, 2459
BspOI	GCTAGC	1 cut(s)	803
BspPI	GGATC	9 cut(s)	299, 485, 871, 884, 1665, 1787, 1800, 1991, 2052
BspQI	GCTCTTC	1 cut(s)	995
BspT107I	GGYRCC	1 cut(s)	540
BspTNI	GGTCTC	1 cut(s)	1169
BsrBI	CCGCTC	1 cut(s)	2525
BsrDI	GCAATG	4 cut(s)	420, 1593, 1938, 2463
BsrI	ACTGG	5 cut(s)	56, 277, 612, 905, 1683
BssECI	CCNNGG	6 cut(s)	243, 303, 820, 997, 1062, 2130
BssNAI	GTATAC	2 cut(s)	898, 1335
BssT1I	CCWWGG	5 cut(s)	243, 303, 997, 1062, 2130
Bst1107I	GTATAC	2 cut(s)	898, 1335
Bst2UI	CCWGG	1 cut(s)	2573
Bst6I	CTCTTC	3 cut(s)	692, 995, 1767
BstAPI	GCANNNNNTGC	3 cut(s)	74, 142, 1195
BstBAI	YACGTR	1 cut(s)	1527
BstC8I	GCNNGC	4 cut(s)	410, 801, 1744, 2011
BstDEI	CTNAG	8 cut(s)	501, 653, 668, 889, 1130, 1652, 1661, 1947
BstDSI	CCRYGG	3 cut(s)	303, 1062, 2130
BstEII	GGTNACC	1 cut(s)	1706
BstF5I	GGATG	5 cut(s)	293, 788, 1387, 1939, 2380
BstH2I	RGCGCY	1 cut(s)	1376
BstHHI	GCGC	2 cut(s)	1375, 1985
BstMAI	GTCTC	7 cut(s)	301, 1169, 1291, 1461, 1800, 1899, 1976
BstNI	CCWGG	1 cut(s)	2573
BstNSI	RCATGY	5 cut(s)	870, 988, 1474, 1901, 2406
BstPI	GGTNACC	1 cut(s)	1706
BstSCI	CCNGG	1 cut(s)	2571
BstSFI	CTRYAG	3 cut(s)	66, 100, 143
BstSNI	TACGTA	1 cut(s)	1527
BstV2I	GAAGAC	3 cut(s)	186, 833, 2592
BstX2I	RGATCY	4 cut(s)	876, 1657, 1792, 2044
BstXI	CCANNNNNNTGG	1 cut(s)	284
BstYI	RGATCY	4 cut(s)	876, 1657, 1792, 2044
BstZ17I	GTATAC	2 cut(s)	898, 1335
Bsu15I	ATCGAT	1 cut(s)	808
BsuRI	GGCC	4 cut(s)	412, 1002, 1851, 2379
BsuTUI	ATCGAT	1 cut(s)	808
BtgI	CCRYGG	3 cut(s)	303, 1062, 2130
BtgZI	GCGATG	1 cut(s)	1767
BtrI	CACGTC	2 cut(s)	399, 831
BtsCI	GGATG	5 cut(s)	293, 788, 1387, 1939, 2380
BtsI	GCAGTG	4 cut(s)	578, 1005, 1569, 2025
BtsIMutI	CAGTG	8 cut(s)	578, 605, 765, 772, 1005, 1541, 1569, 2025
BveI	ACCTGC	2 cut(s)	804, 2459
Cac8I	GCNNGC	4 cut(s)	410, 801, 1744, 2011
CaiI	CAGNNNCTG	3 cut(s)	74, 142, 660
CciI	TCATGA	2 cut(s)	163, 514
CfoI	GCGC	2 cut(s)	1375, 1985
Cfr13I	GGNCC	2 cut(s)	1135, 1511
ClaI	ATCGAT	1 cut(s)	808
Csp6I	GTAC	9 cut(s)	541, 770, 790, 1059, 1528, 1871, 1895, 2120, 2280
CviQI	GTAC	9 cut(s)	541, 770, 790, 1059, 1528, 1871, 1895, 2120, 2280
DdeI	CTNAG	8 cut(s)	501, 653, 668, 889, 1130, 1652, 1661, 1947
DrdI	GACNNNNNNGTC	1 cut(s)	434
DriI	GACNNNNNGTC	1 cut(s)	2593
DseDI	GACNNNNNNGTC	1 cut(s)	434
Eam1104I	CTCTTC	3 cut(s)	692, 995, 1767
Eam1105I	GACNNNNNGTC	1 cut(s)	2593
EarI	CTCTTC	3 cut(s)	692, 995, 1767
EciI	GGCGGA	1 cut(s)	1187
Eco105I	TACGTA	1 cut(s)	1527
Eco130I	CCWWGG	5 cut(s)	243, 303, 997, 1062, 2130
Eco31I	GGTCTC	1 cut(s)	1169
Eco32I	GATATC	2 cut(s)	709, 812
Eco47I	GGWCC	2 cut(s)	1135, 1511
Eco57I	CTGAAG	1 cut(s)	1898
Eco91I	GGTNACC	1 cut(s)	1706
EcoO65I	GGTNACC	1 cut(s)	1706
EcoRI	GAATTC	1 cut(s)	1067
EcoRII	CCWGG	1 cut(s)	2571
EcoRV	GATATC	2 cut(s)	709, 812
EcoT14I	CCWWGG	5 cut(s)	243, 303, 997, 1062, 2130
ErhI	CCWWGG	5 cut(s)	243, 303, 997, 1062, 2130
Esp3I	CGTCTC	1 cut(s)	301
FalI	AAGNNNNNCTT	4 cut(s)	671, 703, 1257, 1289
FaqI	GGGAC	3 cut(s)	6, 1148, 1465
FauNDI	CATATG	2 cut(s)	484, 1232
FbaI	TGATCA	1 cut(s)	37
FblI	GTMKAC	3 cut(s)	897, 1334, 2088
FokI	GGATG	5 cut(s)	300, 795, 1394, 1946, 2367
FspBI	CTAG	5 cut(s)	698, 800, 1577, 2165, 2447
GlaI	GCGC	2 cut(s)	1374, 1984
GsuI	CTGGAG	2 cut(s)	1422, 1485
HaeII	RGCGCY	1 cut(s)	1376
HaeIII	GGCC	4 cut(s)	412, 1002, 1851, 2379
HapII	CCGG	3 cut(s)	1108, 1376, 2260
HhaI	GCGC	2 cut(s)	1375, 1985
Hin6I	GCGC	2 cut(s)	1373, 1983
HinP1I	GCGC	2 cut(s)	1373, 1983
HincII	GTYRAC	3 cut(s)	952, 1734, 2089
HindII	GTYRAC	3 cut(s)	952, 1734, 2089
HindIII	AAGCTT	3 cut(s)	227, 1519, 1649
HpaI	GTTAAC	1 cut(s)	952
HpaII	CCGG	3 cut(s)	1108, 1376, 2260
HphI	GGTGA	1 cut(s)	1718
Hpy166II	GTNNAC	8 cut(s)	175, 370, 772, 898, 952, 1335, 1734, 2089
Hpy8I	GTNNAC	8 cut(s)	175, 370, 772, 898, 952, 1335, 1734, 2089
HpyAV	CCTTC	9 cut(s)	88, 189, 245, 697, 1040, 1318, 1694, 2223, 2510
HpyCH4IV	ACGT	6 cut(s)	398, 432, 830, 1526, 1728, 1893
HpyF3I	CTNAG	8 cut(s)	501, 653, 668, 889, 1130, 1652, 1661, 1947
HpySE526I	ACGT	6 cut(s)	398, 432, 830, 1526, 1728, 1893
HspAI	GCGC	2 cut(s)	1373, 1983
KpnI	GGTACC	1 cut(s)	544
Ksp22I	TGATCA	1 cut(s)	37
KspAI	GTTAAC	1 cut(s)	952
LguI	GCTCTTC	1 cut(s)	995
LmnI	GCTCC	2 cut(s)	202, 2522
MaeI	CTAG	5 cut(s)	698, 800, 1577, 2165, 2447
MaeII	ACGT	6 cut(s)	398, 432, 830, 1526, 1728, 1893
MaeIII	GTNAC	9 cut(s)	257, 504, 754, 1545, 1706, 1889, 1916, 2185, 2506
MbiI	CCGCTC	1 cut(s)	2525
MfeI	CAATTG	1 cut(s)	1254
MflI	RGATCY	4 cut(s)	876, 1657, 1792, 2044
MhlI	GDGCHC	1 cut(s)	923
MlyI	GAGTC	3 cut(s)	1674, 2084, 2528
MmeI	TCCRAC	5 cut(s)	180, 1489, 1648, 1770, 2233
MroXI	GAANNNNTTC	4 cut(s)	220, 1206, 1299, 1353
MseI	TTAA	6 cut(s)	212, 393, 951, 1167, 1623, 2081
MspA1I	CMGCKG	4 cut(s)	65, 105, 142, 2009
MspI	CCGG	3 cut(s)	1108, 1376, 2260
MspR9I	CCNGG	1 cut(s)	2573
MunI	CAATTG	1 cut(s)	1254
Mva1269I	GAATGC	1 cut(s)	1721
MvaI	CCWGG	1 cut(s)	2573
NcoI	CCATGG	3 cut(s)	303, 1062, 2130
NdeI	CATATG	2 cut(s)	484, 1232
NheI	GCTAGC	1 cut(s)	799
NlaIV	GGNNCC	4 cut(s)	542, 878, 1136, 1794
NmeAIII	GCCGAG	1 cut(s)	338
NmuCI	GTSAC	3 cut(s)	754, 1545, 1706
NspI	RCATGY	5 cut(s)	870, 988, 1474, 1901, 2406
PagI	TCATGA	2 cut(s)	163, 514
PaqCI	CACCTGC	1 cut(s)	2459
PciI	ACATGT	3 cut(s)	1470, 1897, 2402
PciSI	GCTCTTC	1 cut(s)	995
PcsI	WCGNNNNNNNCGW	3 cut(s)	188, 194, 933
PctI	GAATGC	1 cut(s)	1721
PdmI	GAANNNNTTC	4 cut(s)	220, 1206, 1299, 1353
PfeI	GAWTC	8 cut(s)	31, 308, 518, 657, 1111, 2098, 2336, 2489
PflFI	GACNNNGTC	1 cut(s)	431
PflMI	CCANNNNNTGG	2 cut(s)	1144, 2386
PleI	GAGTC	3 cut(s)	1673, 2084, 2528
PpsI	GAGTC	3 cut(s)	1673, 2084, 2528
Ppu21I	YACGTR	1 cut(s)	1527
PscI	ACATGT	3 cut(s)	1470, 1897, 2402
Psp6I	CCWGG	1 cut(s)	2571
PspEI	GGTNACC	1 cut(s)	1706
PspGI	CCWGG	1 cut(s)	2571
PspN4I	GGNNCC	4 cut(s)	542, 878, 1136, 1794
PspPI	GGNCC	2 cut(s)	1135, 1511
PstI	CTGCAG	3 cut(s)	70, 104, 147
PstNI	CAGNNNCTG	3 cut(s)	74, 142, 660
PsuI	RGATCY	4 cut(s)	876, 1657, 1792, 2044
PsyI	GACNNNGTC	1 cut(s)	431
PvuII	CAGCTG	3 cut(s)	65, 105, 142
RsaI	GTAC	9 cut(s)	542, 771, 791, 1060, 1529, 1872, 1896, 2121, 2281
RsaNI	GTAC	9 cut(s)	541, 770, 790, 1059, 1528, 1871, 1895, 2120, 2280
SalI	GTCGAC	1 cut(s)	2087
SapI	GCTCTTC	1 cut(s)	995
SaqAI	TTAA	6 cut(s)	212, 393, 951, 1167, 1623, 2081
Sau96I	GGNCC	2 cut(s)	1135, 1511
SchI	GAGTC	3 cut(s)	1674, 2084, 2528
ScrFI	CCNGG	1 cut(s)	2573
SduI	GDGCHC	1 cut(s)	923
SfcI	CTRYAG	3 cut(s)	66, 100, 143
SinI	GGWCC	2 cut(s)	1135, 1511
SmlI	CTYRAG	4 cut(s)	1159, 1288, 1555, 1778
SmoI	CTYRAG	4 cut(s)	1159, 1288, 1555, 1778
SnaBI	TACGTA	1 cut(s)	1527
SsiI	CCGC	6 cut(s)	453, 1172, 1718, 2007, 2031, 2525
SspI	AATATT	1 cut(s)	1143
SspMI	CTAG	5 cut(s)	698, 800, 1577, 2165, 2447
StyD4I	CCNGG	1 cut(s)	2571
StyI	CCWWGG	5 cut(s)	243, 303, 997, 1062, 2130
TaiI	ACGT	6 cut(s)	401, 435, 833, 1529, 1731, 1896
TaqI	TCGA	6 cut(s)	254, 438, 548, 808, 960, 2088
TaqII	GACCGA	1 cut(s)	2243
TatI	WGTACW	3 cut(s)	769, 789, 2279
TauI	GCSGC	3 cut(s)	455, 1721, 2033
TfiI	GAWTC	8 cut(s)	31, 308, 518, 657, 1111, 2098, 2336, 2489
Tru1I	TTAA	6 cut(s)	212, 393, 951, 1167, 1623, 2081
Tru9I	TTAA	6 cut(s)	212, 393, 951, 1167, 1623, 2081
TscAI	CASTG	8 cut(s)	585, 612, 765, 779, 1012, 1548, 1576, 2032
TseFI	GTSAC	3 cut(s)	754, 1545, 1706
Tsp45I	GTSAC	3 cut(s)	754, 1545, 1706
TspRI	CASTG	8 cut(s)	585, 612, 765, 779, 1012, 1548, 1576, 2032
Tth111I	GACNNNGTC	1 cut(s)	431
Van91I	CCANNNNNTGG	2 cut(s)	1144, 2386
VpaK11BI	GGWCC	2 cut(s)	1135, 1511
XapI	RAATTY	3 cut(s)	853, 1067, 2158
XceI	RCATGY	5 cut(s)	870, 988, 1474, 1901, 2406
XmiI	GTMKAC	3 cut(s)	897, 1334, 2088
XmnI	GAANNNNTTC	4 cut(s)	220, 1206, 1299, 1353
XspI	CTAG	5 cut(s)	698, 800, 1577, 2165, 2447

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.