pycom08g21620

Bacterial trigger factor protein (TF)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0011553
Physical Location & Seq
Reverse (-)
21059955 .. 21062347
2393 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 768 bp
ATGTCTAGACAAACAGAGACTCTAGTTGCAACCCGCATTTTACAACACCAAAGCGGCATGGCGATGGCGTCAATGTCTTTGACGATGAACACCACCACGACGATGACGACATCAAAAGTATCTGGATTCCAGCCCCTTTGGCTTCCCAAACGGCATTTTTCTGTTTCTAATCCTGTCAGAAGATACTGCGGTTCCCTTCATCCATCATGCCAATACGCTTCACAGAATCCCAATCTTACGGTTGAGAATCCTAATAACTCTGGCCACGCTCGTTTATCCAGCCCGATATATGCAGCTGCTGGTTCAGGTTTAGAGGCATCAATTGGAGATGCACAAGGCAATCTGATAACCTTGAAAACAGCAAAGATTGTTGTAGAGTCCCAAGACGACAATAAGATACAGGTTAGAGTAGACTTGACTGGAGACGACACACAAAAAGTTTTTGATAGGGTTTTGACAAATTTGGCACGCACAGCACCACCAGTTCCTGGATTTCGCAGACAAAAAGGAGGAAAAACATCCAAGGTCCCGAAAAGCTTTCTGTTGGATATACTTGGTAAGGAGCGTGTCACCAAGTTTGTCATACAAGAAATAATAAACTCTACTATGGCTGATTATGTAAACAAGGAAAACTTGACTGTGAAGGAAAACAAAGTCAGCACCACTCAAACAGCTGAGGAGCTCAAACTGTTGTTCACTCCAGGAAATGAATTTGGATTCAATGCTACACTCGAGCTCGAAACTTCAGAATTGGAGACGCCAAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Trigger_N

Protein Analysis

256

Amino Acids

27.96

Weight (kDa)

9.04

Isoelectric Point (pI)

42.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Trigger_N PF05697 122 - 246 4e-12 Bacterial trigger factor protein (TF)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 488
AccI GTMKAC 1 cut(s) 411
AciI CCGC 3 cut(s) 34, 54, 189
AcoI YGGCCR 1 cut(s) 262
AcsI RAATTY 2 cut(s) 460, 710
AcuI CTGAAG 1 cut(s) 729
AcyI GRCGYC 2 cut(s) 68, 758
AfiI CCNNNNNNNGG 1 cut(s) 488
AgsI TTSAA 2 cut(s) 355, 721
AjnI CCWGG 2 cut(s) 487, 700
AluBI AGCT 6 cut(s) 296, 537, 674, 682, 736, 765
AluI AGCT 6 cut(s) 296, 537, 674, 682, 736, 765
Alw21I GWGCWC 2 cut(s) 684, 738
Alw26I GTCTC 3 cut(s) 11, 417, 749
AlwNI CAGNNNCTG 2 cut(s) 299, 488
Ama87I CYCGRG 1 cut(s) 731
AoxI GGCC 1 cut(s) 262
ApeKI GCWGC 2 cut(s) 293, 296
ApoI RAATTY 2 cut(s) 460, 710
AspS9I GGNCC 1 cut(s) 526
AsuHPI GGTGA 1 cut(s) 562
AvaI CYCGRG 1 cut(s) 731
AvaII GGWCC 1 cut(s) 526
BaeI ACNNNNGTAYC 2 cut(s) 175, 208
BalI TGGCCA 1 cut(s) 264
BanII GRGCYC 2 cut(s) 684, 738
Bbv12I GWGCWC 2 cut(s) 684, 738
BbvCI CCTCAGC 1 cut(s) 675
BbvI GCAGC 2 cut(s) 283, 305
BccI CCATC 2 cut(s) 58, 211
BceAI ACGGC 1 cut(s) 167
BciT130I CCWGG 2 cut(s) 489, 702
BcoDI GTCTC 3 cut(s) 11, 417, 749
BfaI CTAG 2 cut(s) 6, 23
BglI GCCNNNNNGGC 1 cut(s) 139
BisI GCNGC 3 cut(s) 55, 294, 297
BlsI GCNGC 3 cut(s) 56, 295, 298
Bme1390I CCNGG 2 cut(s) 489, 702
Bme18I GGWCC 1 cut(s) 526
BmeT110I CYCGRG 1 cut(s) 731
BmgT120I GGNCC 1 cut(s) 526
BmiI GGNNCC 2 cut(s) 193, 528
BmrFI CCNGG 2 cut(s) 489, 702
BmsI GCATC 2 cut(s) 319, 326
BpmI CTGGAG 2 cut(s) 441, 684
Bpu10I CCTNAGC 1 cut(s) 675
BsaHI GRCGYC 2 cut(s) 68, 758
BsaJI CCNNGG 1 cut(s) 522
Bsc4I CCNNNNNNNGG 1 cut(s) 488
Bse1I ACTGG 2 cut(s) 424, 482
BseBI CCWGG 2 cut(s) 489, 702
BseDI CCNNGG 1 cut(s) 522
BseGI GGATG 2 cut(s) 199, 518
BseLI CCNNNNNNNGG 1 cut(s) 488
BseMII CTCAG 1 cut(s) 666
BseNI ACTGG 2 cut(s) 424, 482
BseRI GAGGAG 1 cut(s) 692
BseXI GCAGC 2 cut(s) 283, 305
BshFI GGCC 1 cut(s) 264
BsiHKAI GWGCWC 2 cut(s) 684, 738
BsiHKCI CYCGRG 1 cut(s) 731
BslFI GGGAC 2 cut(s) 364, 512
BslI CCNNNNNNNGG 1 cut(s) 488
BsmAI GTCTC 3 cut(s) 11, 417, 749
BsmBI CGTCTC 2 cut(s) 417, 749
BsmFI GGGAC 2 cut(s) 364, 512
BsnI GGCC 1 cut(s) 264
BsoBI CYCGRG 1 cut(s) 731
Bsp1286I GDGCHC 2 cut(s) 684, 738
BspACI CCGC 3 cut(s) 34, 54, 189
BspANI GGCC 1 cut(s) 264
BspCNI CTCAG 1 cut(s) 667
BspLI GGNNCC 2 cut(s) 193, 528
BsrI ACTGG 2 cut(s) 424, 482
BssECI CCNNGG 1 cut(s) 522
BssNI GRCGYC 2 cut(s) 68, 758
BssT1I CCWWGG 1 cut(s) 522
Bst2UI CCWGG 2 cut(s) 489, 702
Bst4CI ACNGT 3 cut(s) 241, 640, 690
BstACI GRCGYC 2 cut(s) 68, 758
BstC8I GCNNGC 1 cut(s) 469
BstDEI CTNAG 1 cut(s) 675
BstF5I GGATG 2 cut(s) 199, 518
BstMAI GTCTC 3 cut(s) 11, 417, 749
BstMWI GCNNNNNNNGC 2 cut(s) 139, 473
BstNI CCWGG 2 cut(s) 489, 702
BstSCI CCNGG 2 cut(s) 487, 700
BstV1I GCAGC 2 cut(s) 283, 305
BsuRI GGCC 1 cut(s) 264
BtgZI GCGATG 1 cut(s) 77
BtsCI GGATG 2 cut(s) 199, 518
Cac8I GCNNGC 1 cut(s) 469
CaiI CAGNNNCTG 2 cut(s) 299, 488
Cfr13I GGNCC 1 cut(s) 526
CseI GACGC 1 cut(s) 57
CviAII CATG 2 cut(s) 58, 207
DdeI CTNAG 1 cut(s) 675
EaeI YGGCCR 1 cut(s) 262
Ecl136II GAGCTC 2 cut(s) 682, 736
Eco130I CCWWGG 1 cut(s) 522
Eco24I GRGCYC 2 cut(s) 684, 738
Eco47I GGWCC 1 cut(s) 526
Eco53kI GAGCTC 2 cut(s) 682, 736
Eco57I CTGAAG 1 cut(s) 729
Eco88I CYCGRG 1 cut(s) 731
EcoICRI GAGCTC 2 cut(s) 682, 736
EcoO109I RGGNCCY 1 cut(s) 526
EcoRII CCWGG 2 cut(s) 487, 700
EcoT14I CCWWGG 1 cut(s) 522
EcoT38I GRGCYC 2 cut(s) 684, 738
ErhI CCWWGG 1 cut(s) 522
Esp3I CGTCTC 2 cut(s) 417, 749
FaeI CATG 2 cut(s) 61, 210
FaiI YATR 8 cut(s) 59, 208, 289, 291, 551, 584, 608, 618
FalI AAGNNNNNCTT 2 cut(s) 617, 649
FaqI GGGAC 2 cut(s) 364, 512
FatI CATG 2 cut(s) 57, 206
FauI CCCGC 1 cut(s) 41
FblI GTMKAC 1 cut(s) 411
Fnu4HI GCNGC 3 cut(s) 55, 294, 297
FokI GGATG 2 cut(s) 186, 505
FriOI GRGCYC 2 cut(s) 684, 738
Fsp4HI GCNGC 3 cut(s) 55, 294, 297
FspBI CTAG 2 cut(s) 6, 23
GluI GCNGC 3 cut(s) 55, 294, 297
GsuI CTGGAG 2 cut(s) 441, 684
HaeIII GGCC 1 cut(s) 264
HgaI GACGC 1 cut(s) 57
Hin1I GRCGYC 2 cut(s) 68, 758
Hin1II CATG 2 cut(s) 61, 210
HindIII AAGCTT 1 cut(s) 535
HinfI GANTC 6 cut(s) 19, 126, 226, 247, 377, 717
HphI GGTGA 1 cut(s) 562
Hpy166II GTNNAC 3 cut(s) 412, 622, 696
Hpy188I TCNGA 3 cut(s) 179, 345, 748
Hpy188III TCNNGA 3 cut(s) 6, 123, 529
Hpy8I GTNNAC 3 cut(s) 412, 622, 696
Hpy99I CGWCG 1 cut(s) 103
HpyAV CCTTC 2 cut(s) 206, 637
HpyCH4III ACNGT 3 cut(s) 241, 640, 690
HpyCH4V TGCA 3 cut(s) 29, 293, 332
HpyF10VI GCNNNNNNNGC 2 cut(s) 139, 473
HpyF3I CTNAG 1 cut(s) 675
Hsp92I GRCGYC 2 cut(s) 68, 758
Hsp92II CATG 2 cut(s) 61, 210
LmnI GCTCC 2 cut(s) 562, 679
Lsp1109I GCAGC 2 cut(s) 283, 305
LweI GCATC 2 cut(s) 319, 326
MaeI CTAG 2 cut(s) 6, 23
MaeIII GTNAC 1 cut(s) 568
MboII GAAGA 1 cut(s) 192
MfeI CAATTG 1 cut(s) 321
MhlI GDGCHC 2 cut(s) 684, 738
MlsI TGGCCA 1 cut(s) 264
MluCI AATT 4 cut(s) 321, 460, 710, 749
MluNI TGGCCA 1 cut(s) 264
MlyI GAGTC 2 cut(s) 13, 386
MmeI TCCRAC 1 cut(s) 525
MnlI CCTC 3 cut(s) 307, 503, 670
Mox20I TGGCCA 1 cut(s) 264
MscI TGGCCA 1 cut(s) 264
MslI CAYNNNNRTG 2 cut(s) 62, 101
Msp20I TGGCCA 1 cut(s) 264
MspA1I CMGCKG 2 cut(s) 296, 674
MspR9I CCNGG 2 cut(s) 489, 702
MunI CAATTG 1 cut(s) 321
MvaI CCWGG 2 cut(s) 489, 702
MwoI GCNNNNNNNGC 2 cut(s) 139, 473
NlaIII CATG 2 cut(s) 61, 210
NlaIV GGNNCC 2 cut(s) 193, 528
NmuCI GTSAC 1 cut(s) 568
PaeR7I CTCGAG 1 cut(s) 731
PcsI WCGNNNNNNNCGW 1 cut(s) 104
PfeI GAWTC 4 cut(s) 126, 226, 247, 717
PflMI CCANNNNNTGG 1 cut(s) 488
PfoI TCCNGGA 2 cut(s) 487, 700
PkrI GCNGC 3 cut(s) 56, 295, 298
PleI GAGTC 2 cut(s) 13, 385
PpsI GAGTC 2 cut(s) 13, 385
PpuMI RGGWCCY 1 cut(s) 526
Psp124BI GAGCTC 2 cut(s) 684, 738
Psp5II RGGWCCY 1 cut(s) 526
Psp6I CCWGG 2 cut(s) 487, 700
PspGI CCWGG 2 cut(s) 487, 700
PspN4I GGNNCC 2 cut(s) 193, 528
PspPI GGNCC 1 cut(s) 526
PspPPI RGGWCCY 1 cut(s) 526
PspXI VCTCGAGB 1 cut(s) 731
PstNI CAGNNNCTG 2 cut(s) 299, 488
PvuII CAGCTG 2 cut(s) 296, 674
RseI CAYNNNNRTG 2 cut(s) 62, 101
SacI GAGCTC 2 cut(s) 684, 738
SatI GCNGC 3 cut(s) 55, 294, 297
Sau96I GGNCC 1 cut(s) 526
SchI GAGTC 2 cut(s) 13, 386
ScrFI CCNGG 2 cut(s) 489, 702
SduI GDGCHC 2 cut(s) 684, 738
SfaNI GCATC 2 cut(s) 319, 326
Sfr274I CTCGAG 1 cut(s) 731
SinI GGWCC 1 cut(s) 526
SlaI CTCGAG 1 cut(s) 731
SmiMI CAYNNNNRTG 2 cut(s) 62, 101
SmlI CTYRAG 1 cut(s) 731
SmoI CTYRAG 1 cut(s) 731
Sse9I AATT 4 cut(s) 321, 460, 710, 749
SsiI CCGC 3 cut(s) 34, 54, 189
SspMI CTAG 2 cut(s) 6, 23
SstI GAGCTC 2 cut(s) 684, 738
StyD4I CCNGG 2 cut(s) 487, 700
StyI CCWWGG 1 cut(s) 522
TaaI ACNGT 3 cut(s) 241, 640, 690
TaqI TCGA 2 cut(s) 732, 738
TasI AATT 4 cut(s) 321, 460, 710, 749
TauI GCSGC 1 cut(s) 57
TfiI GAWTC 4 cut(s) 126, 226, 247, 717
TseFI GTSAC 1 cut(s) 568
TseI GCWGC 2 cut(s) 293, 296
Tsp45I GTSAC 1 cut(s) 568
TspDTI ATGAA 3 cut(s) 101, 188, 723
Van91I CCANNNNNTGG 1 cut(s) 488
VpaK11BI GGWCC 1 cut(s) 526
XapI RAATTY 2 cut(s) 460, 710
XbaI TCTAGA 1 cut(s) 5
XhoI CTCGAG 1 cut(s) 731
XmiI GTMKAC 1 cut(s) 411
XspI CTAG 2 cut(s) 6, 23
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.