pycom09g04630

Histidine kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0003045
Physical Location & Seq
Reverse (-)
3412281 .. 3416955
4675 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2775 bp
ATGTGCGATGAACGAGCCCGGATGTTGCAGGATCAGTTTAATGTGAGCCTGAATCATGTTCATGCTTTAGCTATTCTGGTCTCCACCTTTCACCATGGGAAACAACCTACGGCAATTGATCAGAAAACATTTGGTGAATATACAGAGCGAACAGCTTTTGAGAGACCCCTTACGAGTGGTGTTGCTTATGCTTTGAAAGTTACTCATGCCGAGAGGGAACAATTTGAGAAGGAACATGGATGGATAATAAAAAAAATGGAAACTGAGGACCAGAATCTAGTCCAAGATTTTATTACAGAAAGCTTGGCCCCTGCGCCTGTTCAAGATGAATATGCACCCGTGATATTTTCTCAAGAAACTGTCTCCCACATTGTCTCTATTGACATGATGTCCGGAAAGGATGACCGTGAGAACATCTTGCGAGCAAGGGCAACTGGAAAGGGAGTATTGACCTCCCCGTTTAAGCTATTGAAATCCAATCACCTGGGAGTTGTACTCACATTCGCCGTCTATAATGCTGATTTGCCTCCAGATTCCACACCAGAGCAACGAATTCAAGCTACTGTGGGGTATCTAGGTGCATCATACGATGTACCGTCACTGGTGGAGAAGCTCTTGCACCAACTTGCCAGCAAGCAAACAATTGTTGTGAATGTTTATGATACAACTAATTCATCTGCTCCAATCAACATGTATGGTACTGATGTTATTGATACTGGCCTGTTGCACATTAGCAATCTGGATTTTGGGGATCCACATTGTTCACGCAAACATGAAATGCACTGCAGGTTTAAGCACAAACCTCCGTTTCCTTTGACAGCAGCAAGCGCATCAATAGGAGTCCTAGTCATTACCTTCCTAGTTAGTCATATTTTTCATGCGGCCATAAGCCGAATAGCTAAAGTGGAGGCAGACTATCGTGAGATGATGGAACTCAAAGTTCGTGCAGAAGCTGCAGATGTGGCCAAATCTCAGTTCCTTGCAACTGTTTCCCATGAGATCAGGACACCAATGAATGGTGTTTTAGGCATGCTGCAAATGCTGATGGACACCGATCTAGGTCCAAACCAACAGGACTACGCGGAGACTGCTCATGCTAGCGGGAGAGAACTGATATCGCTGATAAATGAGGTTCTTGATCAGGCCAAAATCGAATCAGGCAGGCTGGAACTTGAAACTGTTCCTTTTGATCTGCGGTCAGTGTTTGATAATGTTCTATCACTTAACTCAGGAAAATCCAATGAGAAAGGGATCGAGTTGGCTGCTTATGTCTCCAATATGGTACCTGAAGTTGTCATCGGTGATCCTGGACGCTTTCGGCAGATAATCACAAATCTTGTTGGGAATTCAATTAAGTTCACCCATGACAAAGGACACATATTTGTCTCGGTGCATCTGGTGGATGAAGTGAGGGCCCCACCTGATCTTATGGATGAAGTTCTAAGACAAGGCTTGAACTTAGTTGGAGATGTCTCAAACAAAACTTACAATACATTGAGTGGCTTCCCTGTTGTCAATAGATGGAAAAGCTGGGAAGGTTTTGAATCAATACGCAGCACAACACTGGAGAAATCTGATATGATCAAATTACTTGTAACAGTTGAGGATACAGGTGTAGGAATTCCTCTAGATGCACAAAATCGCATCTTCACACCTTTTATGCAGGCTGATAGCTCCACTTCTAGAACTTATGGTGGTACTGGAATAGGATTGAGCATTAGCAAACGTCTGGTAGATCTCATGAGTGGGGAGATAGGGTTTGTAAGTGAACCTGGCACTGGAAGTACCTTTTCATTTGTTGGAACTTTTGGGAAGGGAGAATTAAGTTCCCTGGAGACAAAGCGGCACCAGTATGAACCAGCTGTCTCAGAGTATCAAGGACTGAGAGCATTGGTAATAGATAATAAAACCATCCGAACTGAGGTCACGAAATACCATATGCGGAGATTGGGAATATCTGTGGATACAGCGTCCTGTCTGGAATCAGCGTGCTCTTATTTATCTAGTGCTGGCAACACCAGTCTTTCCACACCGTTGGCCATGGTTCTTATTGACAAAGATGTTTGGGATAAAGAAACTGGTATTATATTGCATCAGTCACTTAAAGAGCACAGAAAAAACAACAACGTGGAATCGCTTATAAACCTTCCGAAGATACTTCTCGTGGCTACTTCCATCACTCCTGTTGAACGCAATGTAGTAACATCTTTAGGTCTTGTAGATAAAGTATTGACGAAGCCACTTCGGTTAAGTGTCTTAATTGCTTGCTTTCAAGAAGCCCTTGGAAGCCGTAAGAAGAGGCTTCTAAATGCAAACAAGCCAACTCTTGGAAAGTTACTAAGAGAGAAGAGAATTTTGGTGGTGGATGACAATGGAGTGAACAGAAGAGTGGCAGAAGGTGCTTTAAAGAAGTATGGAGCAATTGTCACCTGTGTGGAGAGTGGAAAGGCTGCTTTGTTGAAGCTTAAGCCACCCCACAACTTTGATGCTTGCTTTATGGATCTCCAGATGCCAGAAATGGATGGGTTTGAAGCAACTCGGATCATCCGTGGTATTGAAAGTGAGGTAAATGAGAAAATTGCAGCTGGGGAAGCATCAGTTGAAATGTTTGGCAATGTCGTAAATTGGCACACGCCAATATTGGCTATGACGGCTGATGTGATTCAGGCTTCAAATGAAGAGTGCATAAAATGTGGGATGGATGGTTATGTGTCAAAGCCATTTGAAGAAGACCAGCTGTATTCAGCAGTGGCACGTTTCTTTGAGTCTGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001101 GO:0003674 GO:0003824 GO:0004672 GO:0004673 GO:0004888 GO:0005488 GO:0005515 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006970 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009266 GO:0009409 GO:0009414 GO:0009415 GO:0009628 GO:0009636 GO:0009651 GO:0009719 GO:0009725 GO:0009735 GO:0009736 GO:0009737 GO:0009755 GO:0009884 GO:0009888 GO:0009894 GO:0009909 GO:0009987 GO:0010029 GO:0010033 GO:0010035 GO:0010087 GO:0010271 GO:0010959 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0016775 GO:0018106 GO:0018193 GO:0018202 GO:0019222 GO:0019538 GO:0019899 GO:0019900 GO:0019901 GO:0023052 GO:0031323 GO:0031329 GO:0032502 GO:0032870 GO:0032879 GO:0033554 GO:0033993 GO:0034756 GO:0034757 GO:0036211 GO:0038023 GO:0040007 GO:0042221 GO:0042802 GO:0043170 GO:0043269 GO:0043271 GO:0043412 GO:0043424 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0048509 GO:0048519 GO:0048580 GO:0048831 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051049 GO:0051051 GO:0051171 GO:0051193 GO:0051239 GO:0051716 GO:0060089 GO:0065007 GO:0070417 GO:0070887 GO:0071215 GO:0071229 GO:0071310 GO:0071368 GO:0071396 GO:0071495 GO:0071704 GO:0071944 GO:0080117 GO:0080190 GO:0090056 GO:0097305 GO:0097306 GO:0140096 GO:1900140 GO:1901401 GO:1901404 GO:1901564 GO:1901700 GO:1901701 GO:2000026 GO:2000241
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
CHASE HATPase_c HisKA Response_reg

Protein Analysis

925

Amino Acids

102.03

Weight (kDa)

5.84

Isoelectric Point (pI)

36.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CHASE PF03924 38 - 232 2.1e-36 CHASE domain
HisKA PF00512 322 - 387 5.5e-20 His Kinase A (phospho-acceptor) domain
HATPase_c PF02518 528 - 599 5.6e-18 Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase
Receiver_CRE1 PF24896 623 - 762 4.1e-34 AHK4/CRE1/WOL first receiver domain
Response_reg PF00072 785 - 917 3.7e-18 Response regulator receiver domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2141
Acc36I ACCTGC 1 cut(s) 777
Acc65I GGTACC 1 cut(s) 1282
AccB1I GGYRCC 2 cut(s) 1282, 1845
AccB7I CCANNNNNTGG 2 cut(s) 1016, 2327
AccII CGCG 1 cut(s) 1082
AccIII TCCGGA 1 cut(s) 392
AciI CCGC 6 cut(s) 881, 1082, 1101, 1195, 1843, 1942
AclWI GGATC 7 cut(s) 39, 746, 759, 1259, 1298, 2508, 2549
AcoI YGGCCR 3 cut(s) 882, 963, 2037
AcsI RAATTY 4 cut(s) 552, 1345, 1620, 2352
AcuI CTGAAG 1 cut(s) 1308
AfaI GTAC 6 cut(s) 495, 594, 700, 1284, 1699, 1786
AfiI CCNNNNNNNGG 4 cut(s) 1016, 1778, 1921, 2327
AflII CTTAAG 1 cut(s) 2465
AflIII ACRYGT 1 cut(s) 690
AhdI GACNNNNNGTC 1 cut(s) 388
AjnI CCWGG 4 cut(s) 483, 1306, 1771, 1830
AjuI GAANNNNNNNTTGG 4 cut(s) 959, 991, 2339, 2371
AleI CACNNNNGTG 1 cut(s) 2432
Alw21I GWGCWC 2 cut(s) 1994, 2112
AlwI GGATC 7 cut(s) 39, 746, 759, 1259, 1298, 2508, 2549
AlwNI CAGNNNCTG 1 cut(s) 953
Aor13HI TCCGGA 1 cut(s) 392
AoxI GGCC 7 cut(s) 306, 718, 882, 963, 1143, 1413, 2037
ApaI GGGCCC 1 cut(s) 1417
ApeKI GCWGC 7 cut(s) 821, 953, 1033, 1262, 1554, 2450, 2582
ApoI RAATTY 4 cut(s) 552, 1345, 1620, 2352
Asp700I GAANNNNTTC 1 cut(s) 1179
Asp718I GGTACC 1 cut(s) 1282
AspLEI GCGC 2 cut(s) 316, 830
AspS9I GGNCC 5 cut(s) 268, 307, 1061, 1413, 1414
AsuC2I CCSGG 1 cut(s) 19
AsuHPI GGTGA 6 cut(s) 83, 146, 473, 1313, 1351, 2419
AsuNHI GCTAGC 1 cut(s) 1097
AvaII GGWCC 2 cut(s) 268, 1061
BaeGI GKGCMC 1 cut(s) 1417
BaeI ACNNNNGTAYC 4 cut(s) 690, 723, 1599, 1632
BalI TGGCCA 2 cut(s) 965, 2039
BamHI GGATCC 1 cut(s) 751
BanI GGYRCC 2 cut(s) 1282, 1845
BanII GRGCYC 2 cut(s) 19, 1417
BauI CACGAG 1 cut(s) 2162
BbsI GAAGAC 1 cut(s) 2736
Bbv12I GWGCWC 2 cut(s) 1994, 2112
BbvI GCAGC 7 cut(s) 833, 940, 1020, 1249, 1566, 2437, 2594
BccI CCATC 9 cut(s) 234, 922, 1039, 1515, 1919, 2183, 2516, 2692, 2696
BceAI ACGGC 4 cut(s) 126, 491, 2274, 2667
BcgI CGANNNNNNTGC 2 cut(s) 1244, 1278
BciT130I CCWGG 4 cut(s) 485, 1308, 1773, 1832
BciVI GTATCC 2 cut(s) 1600, 1957
BclI TGATCA 3 cut(s) 118, 1138, 1581
BcnI CCSGG 1 cut(s) 19
BfaI CTAG 9 cut(s) 278, 575, 845, 860, 1058, 1098, 1628, 1683, 2004
BfmI CTRYAG 2 cut(s) 784, 954
BfrI CTTAAG 1 cut(s) 2465
BfuAI ACCTGC 1 cut(s) 777
BfuI GTATCC 2 cut(s) 1600, 1957
BglII AGATCT 1 cut(s) 1735
BisI GCNGC 9 cut(s) 822, 882, 954, 1034, 1263, 1555, 1844, 2451, 2583
BlsI GCNGC 9 cut(s) 823, 883, 955, 1035, 1264, 1556, 1845, 2452, 2584
Bme1390I CCNGG 5 cut(s) 19, 485, 1308, 1773, 1832
Bme18I GGWCC 2 cut(s) 268, 1061
BmeRI GACNNNNNGTC 1 cut(s) 388
BmgT120I GGNCC 5 cut(s) 268, 307, 1061, 1413, 1414
BmiI GGNNCC 6 cut(s) 309, 753, 1284, 1415, 1416, 1847
BmrFI CCNGG 5 cut(s) 19, 485, 1308, 1773, 1832
BmsI GCATC 9 cut(s) 590, 839, 1402, 1621, 1653, 2101, 2476, 2499, 2603
BmtI GCTAGC 1 cut(s) 1101
BpiI GAAGAC 1 cut(s) 2736
BplI GAGNNNNNCTC 2 cut(s) 480, 512
BpmI CTGGAG 4 cut(s) 513, 1586, 1853, 2489
BpuEI CTTGAG 1 cut(s) 336
BpuMI CCSGG 1 cut(s) 19
BsaI GGTCTC 2 cut(s) 85, 157
BsaJI CCNNGG 6 cut(s) 94, 484, 1830, 2040, 2281, 2548
BsaWI WCCGGW 1 cut(s) 392
BsaXI ACNNNNNCTCC 6 cut(s) 831, 861, 1742, 1772, 2409, 2439
Bsc4I CCNNNNNNNGG 4 cut(s) 1016, 1778, 1921, 2327
Bse1I ACTGG 9 cut(s) 439, 606, 721, 1569, 1705, 1783, 1849, 2019, 2083
Bse3DI GCAATG 2 cut(s) 2200, 2620
BseAI TCCGGA 1 cut(s) 392
BseBI CCWGG 4 cut(s) 485, 1308, 1773, 1832
BseDI CCNNGG 6 cut(s) 94, 484, 1830, 2040, 2281, 2548
BseLI CCNNNNNNNGG 4 cut(s) 1016, 1778, 1921, 2327
BseMI GCAATG 2 cut(s) 2200, 2620
BseMII CTCAG 6 cut(s) 255, 986, 1242, 1874, 1881, 1911
BseNI ACTGG 9 cut(s) 439, 606, 721, 1569, 1705, 1783, 1849, 2019, 2083
BseSI GKGCMC 1 cut(s) 1417
BseXI GCAGC 7 cut(s) 833, 940, 1020, 1249, 1566, 2437, 2594
BseYI CCCAGC 2 cut(s) 1530, 2585
BsgI GTGCAG 1 cut(s) 966
Bsh1236I CGCG 1 cut(s) 1082
BshFI GGCC 7 cut(s) 308, 720, 884, 965, 1145, 1415, 2039
BshNI GGYRCC 2 cut(s) 1282, 1845
BsiHKAI GWGCWC 2 cut(s) 1994, 2112
BsiSI CCGG 2 cut(s) 19, 393
BslI CCNNNNNNNGG 4 cut(s) 1016, 1778, 1921, 2327
BsnI GGCC 7 cut(s) 308, 720, 884, 965, 1145, 1415, 2039
Bso31I GGTCTC 2 cut(s) 85, 157
Bsp120I GGGCCC 1 cut(s) 1413
Bsp1286I GDGCHC 4 cut(s) 19, 1417, 1994, 2112
Bsp13I TCCGGA 1 cut(s) 392
Bsp19I CCATGG 2 cut(s) 94, 2040
BspACI CCGC 6 cut(s) 881, 1082, 1101, 1195, 1843, 1942
BspANI GGCC 7 cut(s) 308, 720, 884, 965, 1145, 1415, 2039
BspCNI CTCAG 6 cut(s) 256, 985, 1241, 1875, 1880, 1912
BspEI TCCGGA 1 cut(s) 392
BspFNI CGCG 1 cut(s) 1082
BspHI TCATGA 1 cut(s) 1740
BspLI GGNNCC 6 cut(s) 309, 753, 1284, 1415, 1416, 1847
BspMAI CTGCAG 2 cut(s) 788, 958
BspMI ACCTGC 1 cut(s) 777
BspOI GCTAGC 1 cut(s) 1101
BspPI GGATC 7 cut(s) 39, 746, 759, 1259, 1298, 2508, 2549
BspT107I GGYRCC 2 cut(s) 1282, 1845
BspTI CTTAAG 1 cut(s) 2465
BspTNI GGTCTC 2 cut(s) 85, 157
BsrDI GCAATG 2 cut(s) 2200, 2620
BsrI ACTGG 9 cut(s) 439, 606, 721, 1569, 1705, 1783, 1849, 2019, 2083
BssECI CCNNGG 6 cut(s) 94, 484, 1830, 2040, 2281, 2548
BssSI CACGAG 1 cut(s) 2162
BssT1I CCWWGG 3 cut(s) 94, 2040, 2281
Bst2BI CACGAG 1 cut(s) 2162
Bst2UI CCWGG 4 cut(s) 485, 1308, 1773, 1832
Bst4CI ACNGT 8 cut(s) 361, 407, 565, 597, 988, 1180, 1600, 2034
Bst6I CTCTTC 4 cut(s) 2291, 2342, 2380, 2673
BstAFI CTTAAG 1 cut(s) 2465
BstAPI GCANNNNNTGC 2 cut(s) 953, 2399
BstDEI CTNAG 9 cut(s) 264, 972, 1228, 1442, 1459, 1867, 1883, 1920, 2339
BstDSI CCRYGG 3 cut(s) 94, 2040, 2548
BstFNI CGCG 1 cut(s) 1082
BstHHI GCGC 2 cut(s) 316, 830
BstMWI GCNNNNNNNGC 8 cut(s) 827, 953, 962, 1039, 1088, 2399, 2591, 2651
BstNI CCWGG 4 cut(s) 485, 1308, 1773, 1832
BstNSI RCATGY 2 cut(s) 694, 1033
BstSCI CCNGG 5 cut(s) 17, 483, 1306, 1771, 1830
BstSFI CTRYAG 2 cut(s) 784, 954
BstSLI GKGCMC 1 cut(s) 1417
BstUI CGCG 1 cut(s) 1082
BstV1I GCAGC 7 cut(s) 833, 940, 1020, 1249, 1566, 2437, 2594
BstV2I GAAGAC 1 cut(s) 2736
BstX2I RGATCY 3 cut(s) 751, 1735, 2500
BstXI CCANNNNNNTGG 2 cut(s) 484, 2035
BstYI RGATCY 3 cut(s) 751, 1735, 2500
BsuI GTATCC 2 cut(s) 1600, 1957
BsuRI GGCC 7 cut(s) 308, 720, 884, 965, 1145, 1415, 2039
BtgI CCRYGG 3 cut(s) 94, 2040, 2548
BtgZI GCGATG 1 cut(s) 21
BtsI GCAGTG 2 cut(s) 781, 2754
BtsIMutI CAGTG 6 cut(s) 599, 781, 1206, 1562, 1776, 2754
BveI ACCTGC 1 cut(s) 777
CaiI CAGNNNCTG 1 cut(s) 953
CciI TCATGA 1 cut(s) 1740
CfoI GCGC 2 cut(s) 316, 830
Cfr13I GGNCC 5 cut(s) 268, 307, 1061, 1413, 1414
CseI GACGC 2 cut(s) 1320, 1959
Csp6I GTAC 6 cut(s) 494, 593, 699, 1283, 1698, 1785
CviQI GTAC 6 cut(s) 494, 593, 699, 1283, 1698, 1785
DdeI CTNAG 9 cut(s) 264, 972, 1228, 1442, 1459, 1867, 1883, 1920, 2339
DraI TTTAAA 1 cut(s) 2406
DriI GACNNNNNGTC 1 cut(s) 388
EaeI YGGCCR 3 cut(s) 882, 963, 2037
Eam1104I CTCTTC 4 cut(s) 2291, 2342, 2380, 2673
Eam1105I GACNNNNNGTC 1 cut(s) 388
EarI CTCTTC 4 cut(s) 2291, 2342, 2380, 2673
Eco130I CCWWGG 3 cut(s) 94, 2040, 2281
Eco24I GRGCYC 2 cut(s) 19, 1417
Eco31I GGTCTC 2 cut(s) 85, 157
Eco32I GATATC 1 cut(s) 1116
Eco47I GGWCC 2 cut(s) 268, 1061
Eco57I CTGAAG 1 cut(s) 1308
EcoO109I RGGNCCY 2 cut(s) 1413, 1414
EcoRI GAATTC 3 cut(s) 552, 1345, 1620
EcoRII CCWGG 4 cut(s) 483, 1306, 1771, 1830
EcoRV GATATC 1 cut(s) 1116
EcoT14I CCWWGG 3 cut(s) 94, 2040, 2281
EcoT38I GRGCYC 2 cut(s) 19, 1417
ErhI CCWWGG 3 cut(s) 94, 2040, 2281
FalI AAGNNNNNCTT 2 cut(s) 2265, 2297
FauI CCCGC 1 cut(s) 1094
FauNDI CATATG 1 cut(s) 1938
FbaI TGATCA 3 cut(s) 118, 1138, 1581
Fnu4HI GCNGC 9 cut(s) 822, 882, 954, 1034, 1263, 1555, 1844, 2451, 2583
FriOI GRGCYC 2 cut(s) 19, 1417
Fsp4HI GCNGC 9 cut(s) 822, 882, 954, 1034, 1263, 1555, 1844, 2451, 2583
FspBI CTAG 9 cut(s) 278, 575, 845, 860, 1058, 1098, 1628, 1683, 2004
GlaI GCGC 2 cut(s) 315, 829
GluI GCNGC 9 cut(s) 822, 882, 954, 1034, 1263, 1555, 1844, 2451, 2583
GsaI CCCAGC 2 cut(s) 1534, 2589
GsuI CTGGAG 4 cut(s) 513, 1586, 1853, 2489
HaeIII GGCC 7 cut(s) 308, 720, 884, 965, 1145, 1415, 2039
HapII CCGG 2 cut(s) 19, 393
HgaI GACGC 2 cut(s) 1320, 1959
HhaI GCGC 2 cut(s) 316, 830
Hin6I GCGC 2 cut(s) 314, 828
HinP1I GCGC 2 cut(s) 314, 828
HindIII AAGCTT 2 cut(s) 301, 2462
HpaII CCGG 2 cut(s) 19, 393
HphI GGTGA 6 cut(s) 83, 146, 473, 1313, 1351, 2419
Hpy166II GTNNAC 4 cut(s) 764, 1359, 1769, 2380
Hpy188I TCNGA 6 cut(s) 123, 1576, 1870, 1916, 2151, 2541
Hpy8I GTNNAC 4 cut(s) 764, 1359, 1769, 2380
HpyAV CCTTC 6 cut(s) 223, 865, 1529, 1807, 2156, 2390
HpyCH4III ACNGT 8 cut(s) 361, 407, 565, 597, 988, 1180, 1600, 2034
HpyCH4IV ACGT 3 cut(s) 1726, 2127, 2755
HpyF10VI GCNNNNNNNGC 8 cut(s) 827, 953, 962, 1039, 1088, 2399, 2591, 2651
HpyF3I CTNAG 9 cut(s) 264, 972, 1228, 1442, 1459, 1867, 1883, 1920, 2339
HpySE526I ACGT 3 cut(s) 1726, 2127, 2755
HspAI GCGC 2 cut(s) 314, 828
Kpn2I TCCGGA 1 cut(s) 392
KpnI GGTACC 1 cut(s) 1286
Ksp22I TGATCA 3 cut(s) 118, 1138, 1581
LmnI GCTCC 3 cut(s) 685, 1679, 2417
Lsp1109I GCAGC 7 cut(s) 833, 940, 1020, 1249, 1566, 2437, 2594
LweI GCATC 9 cut(s) 590, 839, 1402, 1621, 1653, 2101, 2476, 2499, 2603
MaeI CTAG 9 cut(s) 278, 575, 845, 860, 1058, 1098, 1628, 1683, 2004
MaeII ACGT 3 cut(s) 1726, 2127, 2755
MaeIII GTNAC 8 cut(s) 199, 597, 1594, 1924, 2097, 2200, 2334, 2425
MboII GAAGA 8 cut(s) 1639, 2164, 2308, 2359, 2397, 2690, 2738, 2741
MfeI CAATTG 3 cut(s) 114, 642, 2421
MflI RGATCY 3 cut(s) 751, 1735, 2500
MhlI GDGCHC 4 cut(s) 19, 1417, 1994, 2112
MlsI TGGCCA 2 cut(s) 965, 2039
MluNI TGGCCA 2 cut(s) 965, 2039
MlyI GAGTC 2 cut(s) 849, 2774
MmeI TCCRAC 2 cut(s) 1444, 1780
Mox20I TGGCCA 2 cut(s) 965, 2039
MroI TCCGGA 1 cut(s) 392
MroXI GAANNNNTTC 1 cut(s) 1179
MscI TGGCCA 2 cut(s) 965, 2039
MslI CAYNNNNRTG 3 cut(s) 60, 1851, 2432
Msp20I TGGCCA 2 cut(s) 965, 2039
MspA1I CMGCKG 3 cut(s) 1862, 2585, 2737
MspCI CTTAAG 1 cut(s) 2465
MspI CCGG 2 cut(s) 19, 393
MspR9I CCNGG 5 cut(s) 19, 485, 1308, 1773, 1832
MunI CAATTG 3 cut(s) 114, 642, 2421
MvaI CCWGG 4 cut(s) 485, 1308, 1773, 1832
MvnI CGCG 1 cut(s) 1082
MwoI GCNNNNNNNGC 8 cut(s) 827, 953, 962, 1039, 1088, 2399, 2591, 2651
NciI CCSGG 1 cut(s) 19
NcoI CCATGG 2 cut(s) 94, 2040
NdeI CATATG 1 cut(s) 1938
NheI GCTAGC 1 cut(s) 1097
NlaIV GGNNCC 6 cut(s) 309, 753, 1284, 1415, 1416, 1847
NmeAIII GCCGAG 1 cut(s) 235
NmuCI GTSAC 4 cut(s) 597, 1924, 2097, 2425
NspI RCATGY 2 cut(s) 694, 1033
OliI CACNNNNGTG 1 cut(s) 2432
PaeI GCATGC 1 cut(s) 1033
PagI TCATGA 1 cut(s) 1740
PciI ACATGT 1 cut(s) 690
PcsI WCGNNNNNNNCGW 1 cut(s) 2545
PdmI GAANNNNTTC 1 cut(s) 1179
PfeI GAWTC 8 cut(s) 52, 274, 533, 1154, 1544, 1982, 2132, 2662
PflMI CCANNNNNTGG 2 cut(s) 1016, 2327
PfoI TCCNGGA 1 cut(s) 1306
PkrI GCNGC 9 cut(s) 823, 883, 955, 1035, 1264, 1556, 1845, 2452, 2584
PleI GAGTC 2 cut(s) 848, 2773
PpsI GAGTC 2 cut(s) 848, 2773
PscI ACATGT 1 cut(s) 690
PsiI TTATAA 1 cut(s) 2141
Psp6I CCWGG 4 cut(s) 483, 1306, 1771, 1830
PspFI CCCAGC 2 cut(s) 1530, 2585
PspGI CCWGG 4 cut(s) 483, 1306, 1771, 1830
PspN4I GGNNCC 6 cut(s) 309, 753, 1284, 1415, 1416, 1847
PspOMI GGGCCC 1 cut(s) 1413
PspPI GGNCC 5 cut(s) 268, 307, 1061, 1413, 1414
PstI CTGCAG 2 cut(s) 788, 958
PstNI CAGNNNCTG 1 cut(s) 953
PsuI RGATCY 3 cut(s) 751, 1735, 2500
PvuII CAGCTG 3 cut(s) 1862, 2585, 2737
RsaI GTAC 6 cut(s) 495, 594, 700, 1284, 1699, 1786
RsaNI GTAC 6 cut(s) 494, 593, 699, 1283, 1698, 1785
RseI CAYNNNNRTG 3 cut(s) 60, 1851, 2432
SatI GCNGC 9 cut(s) 822, 882, 954, 1034, 1263, 1555, 1844, 2451, 2583
Sau96I GGNCC 5 cut(s) 268, 307, 1061, 1413, 1414
SchI GAGTC 2 cut(s) 849, 2774
ScrFI CCNGG 5 cut(s) 19, 485, 1308, 1773, 1832
SduI GDGCHC 4 cut(s) 19, 1417, 1994, 2112
SfaNI GCATC 9 cut(s) 590, 839, 1402, 1621, 1653, 2101, 2476, 2499, 2603
SfcI CTRYAG 2 cut(s) 784, 954
SinI GGWCC 2 cut(s) 268, 1061
SmiMI CAYNNNNRTG 3 cut(s) 60, 1851, 2432
SmlI CTYRAG 2 cut(s) 351, 2465
SmoI CTYRAG 2 cut(s) 351, 2465
SphI GCATGC 1 cut(s) 1033
SsiI CCGC 6 cut(s) 881, 1082, 1101, 1195, 1843, 1942
SspI AATATT 1 cut(s) 2640
SspMI CTAG 9 cut(s) 278, 575, 845, 860, 1058, 1098, 1628, 1683, 2004
StyD4I CCNGG 5 cut(s) 17, 483, 1306, 1771, 1830
StyI CCWWGG 3 cut(s) 94, 2040, 2281
TaaI ACNGT 8 cut(s) 361, 407, 565, 597, 988, 1180, 1600, 2034
TaiI ACGT 3 cut(s) 1729, 2130, 2758
TaqI TCGA 2 cut(s) 1152, 1254
TatI WGTACW 1 cut(s) 493
TauI GCSGC 2 cut(s) 884, 1846
TfiI GAWTC 8 cut(s) 52, 274, 533, 1154, 1544, 1982, 2132, 2662
TscAI CASTG 6 cut(s) 606, 788, 1206, 1569, 1783, 2754
TseFI GTSAC 4 cut(s) 597, 1924, 2097, 2425
TseI GCWGC 7 cut(s) 821, 953, 1033, 1262, 1554, 2450, 2582
Tsp45I GTSAC 4 cut(s) 597, 1924, 2097, 2425
TspGWI ACGGA 2 cut(s) 795, 2537
TspRI CASTG 6 cut(s) 606, 788, 1206, 1569, 1783, 2754
Van91I CCANNNNNTGG 2 cut(s) 1016, 2327
Vha464I CTTAAG 1 cut(s) 2465
VpaK11BI GGWCC 2 cut(s) 268, 1061
XapI RAATTY 4 cut(s) 552, 1345, 1620, 2352
XbaI TCTAGA 2 cut(s) 1627, 1682
XceI RCATGY 2 cut(s) 694, 1033
XmnI GAANNNNTTC 1 cut(s) 1179
XspI CTAG 9 cut(s) 278, 575, 845, 860, 1058, 1098, 1628, 1683, 2004
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.