pycom09g06080

pectinesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0012811
Physical Location & Seq
Forward (+)
4497425 .. 4498945
1521 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1050 bp
ATGAGTCTCAAAACCACTTTCCTAACATATTTTTTTGCCATTTTTGCAATTTTATTGGCATCTCGAAATCTCATAAGTATTCCAAAATCGTCATCCCTCAAATACTTTGTCAACCTTAGTGATCTCACCACCTCATCCTCAGTGCCCAATATTATTTCTCTTCTCATCCCACAAAACATATTTGCTAGGCATCACAAAAAACATGAGGATGGGGGAAAAATAGGATCCATTTGTGATGGATTTCCACGTGGTTTTCCTCCTCCAGACACCAACACAACCTCCTACCTTTGTGTTGATCGAAATGGGTGTTGCAATTTCACATTGGTTCAAGCAGCTGTTGATGCAGTTCCAACTCCTAGCCCGAAGAGAACAATAATTTGGATTAATAAGGGCATTTACTATCAGCTTCAGTTTTTTTTCACAGTTTTGGGTGAAAAAAAGCCAAAAAACAAGAAGCTGCAAAACCCAGCTTTGAAAAATCGGTTTTTTTTCACATCTGTTTTACATAAAAGTTTACCAAATAGTATAATACTGTTTTTTTTCTTTTCAAAAGCACTTTTACAAAAAAGTTTACCAAACACTCTGCTGCTTTATTTCACAGCCGCTTATTCTCACAGCACAGCAGAATCAAAAGTCACGGTTCCAAAAACCAAACCAAATATAACATTTCAAGGACAAGGGTATGAAACGACCGCAATATCATGGAACGACACAGCCAATTCATCGCATGGCACCTTTTACAGTGGCTCAGTTCAAGTTTTTGCTGACAACTTTGTTGCTAAGAACATAAGCTTCATGAATGTGGCTCCAATTCCGAAGCCTGGAGATGTGGGAGCTCAAGCTGTAGCGATCAGAATATCAGGAGACCAAGCAGCATTCTTGGGTTGTGGGTTCTTTGGAGCTCAAGACACCCTTCACGATGATAGGGGCCGCCATTACTTTAAGGATTGCTATATCCAGGGCTCCATTGATTTCATCTTTGGCAATGGAAGGTCATTGTATGAGGTAATCCTCCTCATTTCTGATATGGAATTTTGTTTAATCCTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Pectinesterase

Protein Analysis

350

Amino Acids

38.77

Weight (kDa)

9.27

Isoelectric Point (pI)

33.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pectinesterase PF01095 209 - 334 2.8e-29 Pectinesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes (Group: OG0012811)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G05310
fragaria_vesca FvH4_6g39490
malus_domestica MD09G1139900.v1.1 MD17G1129300.v1.1
prunus_persica Prupe.3G185700_v2.0.a1 Prupe.3G185700_v2.0.a1
pyrus_communis pycom09g06080 pycom17g12170
rosa_chinensis RchiOBHm_Chr2g0153521
rosa_laevigata RLG00000020705
rosa_multiflora Rmu_sc0003976.1_g000011
rosa_roxburghii Rroxscaffold_2G00094960
rosa_rugosa Rorug02G0438800
rosa_samantha Rh2AG502700 Rh2BG513500 Rh2CG488500 Rh2DG525200
rosa_wichuraiana Rw2G041280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 731
AciI CCGC 3 cut(s) 603, 693, 931
AclWI GGATC 2 cut(s) 219, 232
AcsI RAATTY 1 cut(s) 1031
AcuI CTGAAG 1 cut(s) 392
AcvI CACGTG 1 cut(s) 248
AfiI CCNNNNNNNGG 1 cut(s) 821
AgsI TTSAA 5 cut(s) 329, 475, 549, 671, 755
AjnI CCWGG 2 cut(s) 820, 957
AjuI GAANNNNNNNTTGG 2 cut(s) 361, 393
AluBI AGCT 8 cut(s) 335, 406, 457, 470, 792, 836, 842, 902
AluI AGCT 8 cut(s) 335, 406, 457, 470, 792, 836, 842, 902
Alw21I GWGCWC 2 cut(s) 838, 904
Alw26I GTCTC 2 cut(s) 11, 858
AlwI GGATC 2 cut(s) 219, 232
AoxI GGCC 1 cut(s) 928
ApeKI GCWGC 4 cut(s) 332, 457, 586, 872
ApoI RAATTY 1 cut(s) 1031
ArsI GACNNNNNNTTYG 2 cut(s) 93, 125
AseI ATTAAT 1 cut(s) 384
AspS9I GGNCC 1 cut(s) 928
AsuHPI GGTGA 2 cut(s) 118, 443
BaeGI GKGCMC 1 cut(s) 147
BamHI GGATCC 1 cut(s) 224
BanI GGYRCC 1 cut(s) 731
BanII GRGCYC 3 cut(s) 838, 904, 965
BbrPI CACGTG 1 cut(s) 248
Bbv12I GWGCWC 2 cut(s) 838, 904
BbvI GCAGC 4 cut(s) 344, 444, 573, 884
BccI CCATC 2 cut(s) 203, 230
BciT130I CCWGG 2 cut(s) 822, 959
BcoDI GTCTC 2 cut(s) 11, 858
BfaI CTAG 2 cut(s) 186, 357
BfmI CTRYAG 1 cut(s) 843
BisI GCNGC 6 cut(s) 333, 458, 587, 603, 873, 931
BlsI GCNGC 6 cut(s) 334, 459, 588, 604, 874, 932
Bme1390I CCNGG 2 cut(s) 822, 959
BmgT120I GGNCC 1 cut(s) 928
BmiI GGNNCC 6 cut(s) 226, 642, 733, 807, 929, 964
BmrFI CCNGG 2 cut(s) 822, 959
BmsI GCATC 3 cut(s) 68, 199, 331
BpmI CTGGAG 2 cut(s) 246, 843
BpuEI CTTGAG 2 cut(s) 822, 888
BsaAI YACGTR 1 cut(s) 248
BsaI GGTCTC 1 cut(s) 858
BsaJI CCNNGG 1 cut(s) 958
BsaXI ACNNNNNCTCC 2 cut(s) 263, 293
Bsc4I CCNNNNNNNGG 1 cut(s) 821
Bse3DI GCAATG 1 cut(s) 991
BseBI CCWGG 2 cut(s) 822, 959
BseDI CCNNGG 1 cut(s) 958
BseGI GGATG 4 cut(s) 92, 134, 165, 214
BseLI CCNNNNNNNGG 1 cut(s) 821
BseMI GCAATG 1 cut(s) 991
BseMII CTCAG 2 cut(s) 153, 762
BseRI GAGGAG 2 cut(s) 249, 1004
BseSI GKGCMC 1 cut(s) 147
BseXI GCAGC 4 cut(s) 344, 444, 573, 884
BseYI CCCAGC 1 cut(s) 466
Bsh1285I CGRYCG 1 cut(s) 693
BshFI GGCC 1 cut(s) 930
BshNI GGYRCC 1 cut(s) 731
BsiEI CGRYCG 1 cut(s) 693
BsiHKAI GWGCWC 2 cut(s) 838, 904
BslI CCNNNNNNNGG 1 cut(s) 821
BsmAI GTCTC 2 cut(s) 11, 858
BsmI GAATGC 1 cut(s) 875
BsnI GGCC 1 cut(s) 930
Bso31I GGTCTC 1 cut(s) 858
Bsp1286I GDGCHC 4 cut(s) 147, 838, 904, 965
Bsp143I GATC 4 cut(s) 121, 224, 295, 849
BspACI CCGC 3 cut(s) 603, 693, 931
BspANI GGCC 1 cut(s) 930
BspCNI CTCAG 2 cut(s) 152, 761
BspHI TCATGA 1 cut(s) 795
BspLI GGNNCC 6 cut(s) 226, 642, 733, 807, 929, 964
BspPI GGATC 2 cut(s) 219, 232
BspT107I GGYRCC 1 cut(s) 731
BspTNI GGTCTC 1 cut(s) 858
BsrDI GCAATG 1 cut(s) 991
BssECI CCNNGG 1 cut(s) 958
BssMI GATC 4 cut(s) 121, 224, 295, 849
Bst2UI CCWGG 2 cut(s) 822, 959
Bst4CI ACNGT 4 cut(s) 424, 534, 640, 743
Bst6I CTCTTC 2 cut(s) 165, 359
BstBAI YACGTR 1 cut(s) 248
BstDEI CTNAG 4 cut(s) 116, 139, 748, 780
BstF5I GGATG 4 cut(s) 92, 134, 165, 214
BstKTI GATC 4 cut(s) 124, 227, 298, 852
BstMAI GTCTC 2 cut(s) 11, 858
BstMBI GATC 4 cut(s) 121, 224, 295, 849
BstMCI CGRYCG 1 cut(s) 693
BstMWI GCNNNNNNNGC 2 cut(s) 44, 341
BstNI CCWGG 2 cut(s) 822, 959
BstSCI CCNGG 2 cut(s) 820, 957
BstSFI CTRYAG 1 cut(s) 843
BstSLI GKGCMC 1 cut(s) 147
BstV1I GCAGC 4 cut(s) 344, 444, 573, 884
BstX2I RGATCY 1 cut(s) 224
BstYI RGATCY 1 cut(s) 224
BsuRI GGCC 1 cut(s) 930
BtgZI GCGATG 1 cut(s) 708
BtsCI GGATG 4 cut(s) 92, 134, 165, 214
BtsIMutI CAGTG 2 cut(s) 147, 748
CciI TCATGA 1 cut(s) 795
Cfr13I GGNCC 1 cut(s) 928
CviAII CATG 4 cut(s) 203, 702, 728, 796
DdeI CTNAG 4 cut(s) 116, 139, 748, 780
DpnI GATC 4 cut(s) 123, 226, 297, 851
DpnII GATC 4 cut(s) 121, 224, 295, 849
Eam1104I CTCTTC 2 cut(s) 165, 359
EarI CTCTTC 2 cut(s) 165, 359
Ecl136II GAGCTC 2 cut(s) 836, 902
Eco24I GRGCYC 3 cut(s) 838, 904, 965
Eco31I GGTCTC 1 cut(s) 858
Eco53kI GAGCTC 2 cut(s) 836, 902
Eco57I CTGAAG 1 cut(s) 392
Eco72I CACGTG 1 cut(s) 248
EcoICRI GAGCTC 2 cut(s) 836, 902
EcoRII CCWGG 2 cut(s) 820, 957
EcoT38I GRGCYC 3 cut(s) 838, 904, 965
FaeI CATG 4 cut(s) 206, 705, 731, 799
FalI AAGNNNNNCTT 2 cut(s) 897, 929
FatI CATG 4 cut(s) 202, 701, 727, 795
Fnu4HI GCNGC 6 cut(s) 333, 458, 587, 603, 873, 931
FokI GGATG 4 cut(s) 79, 121, 152, 221
FriOI GRGCYC 3 cut(s) 838, 904, 965
Fsp4HI GCNGC 6 cut(s) 333, 458, 587, 603, 873, 931
FspBI CTAG 2 cut(s) 186, 357
GluI GCNGC 6 cut(s) 333, 458, 587, 603, 873, 931
GsaI CCCAGC 1 cut(s) 470
GsuI CTGGAG 2 cut(s) 246, 843
HaeIII GGCC 1 cut(s) 930
Hin1II CATG 4 cut(s) 206, 705, 731, 799
HincII GTYRAC 1 cut(s) 112
HindII GTYRAC 1 cut(s) 112
HindIII AAGCTT 1 cut(s) 790
HinfI GANTC 2 cut(s) 4, 626
HphI GGTGA 2 cut(s) 118, 443
Hpy166II GTNNAC 3 cut(s) 112, 515, 572
Hpy188I TCNGA 3 cut(s) 816, 854, 1024
Hpy188III TCNNGA 6 cut(s) 63, 263, 796, 861, 905, 917
Hpy8I GTNNAC 3 cut(s) 112, 515, 572
HpyAV CCTTC 2 cut(s) 923, 984
HpyCH4III ACNGT 4 cut(s) 424, 534, 640, 743
HpyCH4IV ACGT 1 cut(s) 247
HpyCH4V TGCA 4 cut(s) 47, 312, 344, 460
HpyF10VI GCNNNNNNNGC 2 cut(s) 44, 341
HpyF3I CTNAG 4 cut(s) 116, 139, 748, 780
HpySE526I ACGT 1 cut(s) 247
Hsp92II CATG 4 cut(s) 206, 705, 731, 799
Kzo9I GATC 4 cut(s) 121, 224, 295, 849
LmnI GCTCC 4 cut(s) 811, 833, 899, 968
LpnPI CCDG 7 cut(s) 276, 480, 807, 834, 846, 944, 971
Lsp1109I GCAGC 4 cut(s) 344, 444, 573, 884
LweI GCATC 3 cut(s) 68, 199, 331
MaeI CTAG 2 cut(s) 186, 357
MaeII ACGT 1 cut(s) 247
MaeIII GTNAC 1 cut(s) 634
MalI GATC 4 cut(s) 123, 226, 297, 851
MboI GATC 4 cut(s) 121, 224, 295, 849
MboII GAAGA 2 cut(s) 152, 376
MflI RGATCY 1 cut(s) 224
MhlI GDGCHC 4 cut(s) 147, 838, 904, 965
MluCI AATT 6 cut(s) 48, 313, 375, 718, 810, 1031
MlyI GAGTC 1 cut(s) 13
MmeI TCCRAC 1 cut(s) 374
MseI TTAA 3 cut(s) 384, 942, 1040
MslI CAYNNNNRTG 2 cut(s) 207, 800
MspA1I CMGCKG 1 cut(s) 335
MspR9I CCNGG 2 cut(s) 822, 959
Mva1269I GAATGC 1 cut(s) 875
MvaI CCWGG 2 cut(s) 822, 959
MwoI GCNNNNNNNGC 2 cut(s) 44, 341
NdeII GATC 4 cut(s) 121, 224, 295, 849
NlaIII CATG 4 cut(s) 206, 705, 731, 799
NlaIV GGNNCC 6 cut(s) 226, 642, 733, 807, 929, 964
NmuCI GTSAC 1 cut(s) 634
PagI TCATGA 1 cut(s) 795
PctI GAATGC 1 cut(s) 875
PfeI GAWTC 1 cut(s) 626
PkrI GCNGC 6 cut(s) 334, 459, 588, 604, 874, 932
PleI GAGTC 1 cut(s) 12
PmaCI CACGTG 1 cut(s) 248
PmlI CACGTG 1 cut(s) 248
PpsI GAGTC 1 cut(s) 12
Ppu21I YACGTR 1 cut(s) 248
PshBI ATTAAT 1 cut(s) 384
Psp124BI GAGCTC 2 cut(s) 838, 904
Psp6I CCWGG 2 cut(s) 820, 957
PspCI CACGTG 1 cut(s) 248
PspFI CCCAGC 1 cut(s) 466
PspGI CCWGG 2 cut(s) 820, 957
PspN4I GGNNCC 6 cut(s) 226, 642, 733, 807, 929, 964
PspPI GGNCC 1 cut(s) 928
PsuI RGATCY 1 cut(s) 224
PvuII CAGCTG 1 cut(s) 335
RseI CAYNNNNRTG 2 cut(s) 207, 800
SacI GAGCTC 2 cut(s) 838, 904
SaqAI TTAA 3 cut(s) 384, 942, 1040
SatI GCNGC 6 cut(s) 333, 458, 587, 603, 873, 931
Sau3AI GATC 4 cut(s) 121, 224, 295, 849
Sau96I GGNCC 1 cut(s) 928
SchI GAGTC 1 cut(s) 13
ScrFI CCNGG 2 cut(s) 822, 959
SduI GDGCHC 4 cut(s) 147, 838, 904, 965
SfaNI GCATC 3 cut(s) 68, 199, 331
SfcI CTRYAG 1 cut(s) 843
SmiMI CAYNNNNRTG 2 cut(s) 207, 800
SmlI CTYRAG 2 cut(s) 837, 903
SmoI CTYRAG 2 cut(s) 837, 903
Sse9I AATT 6 cut(s) 48, 313, 375, 718, 810, 1031
SsiI CCGC 3 cut(s) 603, 693, 931
SspI AATATT 1 cut(s) 151
SspMI CTAG 2 cut(s) 186, 357
SstI GAGCTC 2 cut(s) 838, 904
StyD4I CCNGG 2 cut(s) 820, 957
TaaI ACNGT 4 cut(s) 424, 534, 640, 743
TaiI ACGT 1 cut(s) 250
TaqI TCGA 2 cut(s) 64, 298
TasI AATT 6 cut(s) 48, 313, 375, 718, 810, 1031
TauI GCSGC 2 cut(s) 605, 933
TfiI GAWTC 1 cut(s) 626
Tru1I TTAA 3 cut(s) 384, 942, 1040
Tru9I TTAA 3 cut(s) 384, 942, 1040
TscAI CASTG 2 cut(s) 147, 748
TseFI GTSAC 1 cut(s) 634
TseI GCWGC 4 cut(s) 332, 457, 586, 872
Tsp45I GTSAC 1 cut(s) 634
TspDTI ATGAA 5 cut(s) 699, 711, 784, 812, 964
TspRI CASTG 2 cut(s) 147, 748
VspI ATTAAT 1 cut(s) 384
XapI RAATTY 1 cut(s) 1031
XspI CTAG 2 cut(s) 186, 357
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.