pycom09g12290

Early nodulin-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0016703
Physical Location & Seq
Forward (+)
10739210 .. 10740229
1020 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 549 bp
ATGTCTTCTTTCTTGTTTCTCTTCCATGCTTCGCTGACGGTCTTCTTGGCCGTCACAAATTGTCCAGTTGAAGCTGCAAAGGAGTTCCGAGTGGGTGATGATTTGGGATGGAGGCAACCCGGCTTGAACGACACGGATGTGTACTCTCAATGGGCTGCCGGGAAGAGATTTCATGTCGGAGATTCTCTTTTTCTTGAGGTTGACAAATGGGATTATTACCACTGCAACACAAGCAATCCCATCATCGACTTTGATAACGGCAAGAGCATCATGAAGCTCGATAGGCCTGGCCCTTTCTATTTCATCAGCGGAGCTCCTGATCATTGCAAGAACGGGCAGAGACTTTTTGTCGAGGTGATTGATCTTCCCCCGATCGTGCAGTCTCCTCCACCCTTTGCCGCCCCACCTGAACAATATTTGCCTGACGACTCTCCTGCACCTGCACCCTCGCCAAGCTTAGGAATTGCCATTTTGATTACTCCTTGGTCGCTTGTTATGCCTCTCATCATCACACTTTTGGCTTTAGTAGCGCGCTCCTCAGCACTCTAG

Protein Analysis

183

Amino Acids

20.07

Weight (kDa)

4.87

Isoelectric Point (pI)

50.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu_bind_like PF02298 46 - 113 9.3e-16 Plastocyanin-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0016703)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G79800
fragaria_vesca FvH4_6g32140
malus_domestica MD09G1206100.v1.1
prunus_persica Prupe.3G061700_v2.0.a1 Prupe.3G062100_v2.0.a1
pyrus_communis pycom09g12290
rosa_laevigata RLG00000019843
rosa_roxburghii Rroxscaffold_2G00105130
rosa_rugosa Rorug02G0360500
rosa_samantha Rh2AG411200 Rh2BG421400 Rh2CG397300 Rh2DG430900
rosa_wichuraiana Rw2G033680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 448
Acc36I ACCTGC 1 cut(s) 448
AccII CGCG 1 cut(s) 532
AciI CCGC 2 cut(s) 309, 399
AcoI YGGCCR 1 cut(s) 48
AfaI GTAC 1 cut(s) 143
AfiI CCNNNNNNNGG 1 cut(s) 458
AgsI TTSAA 2 cut(s) 71, 127
AhdI GACNNNNNGTC 1 cut(s) 347
AjnI CCWGG 1 cut(s) 286
AleI CACNNNNGTG 1 cut(s) 137
AluBI AGCT 4 cut(s) 74, 277, 314, 456
AluI AGCT 4 cut(s) 74, 277, 314, 456
Alw21I GWGCWC 1 cut(s) 316
Alw26I GTCTC 2 cut(s) 334, 387
AoxI GGCC 3 cut(s) 48, 284, 289
ApeKI GCWGC 2 cut(s) 74, 155
AspLEI GCGC 2 cut(s) 532, 534
AspS9I GGNCC 1 cut(s) 290
AsuC2I CCSGG 2 cut(s) 120, 160
AsuHPI GGTGA 2 cut(s) 107, 367
BanII GRGCYC 1 cut(s) 316
BbsI GAAGAC 1 cut(s) 34
Bbv12I GWGCWC 1 cut(s) 316
BbvCI CCTCAGC 1 cut(s) 538
BbvI GCAGC 2 cut(s) 61, 142
BccI CCATC 2 cut(s) 102, 248
BceAI ACGGC 2 cut(s) 35, 274
BcgI CGANNNNNNTGC 2 cut(s) 416, 450
BciT130I CCWGG 1 cut(s) 288
BclI TGATCA 1 cut(s) 319
BcnI CCSGG 2 cut(s) 120, 160
BcoDI GTCTC 2 cut(s) 334, 387
BfaI CTAG 1 cut(s) 547
BfuAI ACCTGC 1 cut(s) 448
BisI GCNGC 3 cut(s) 75, 156, 399
BlsI GCNGC 3 cut(s) 76, 157, 400
Bme1390I CCNGG 3 cut(s) 120, 160, 288
BmeRI GACNNNNNGTC 1 cut(s) 347
BmgT120I GGNCC 1 cut(s) 290
BmrFI CCNGG 3 cut(s) 120, 160, 288
BmsI GCATC 1 cut(s) 276
BpiI GAAGAC 1 cut(s) 34
Bpu10I CCTNAGC 2 cut(s) 457, 538
BpuEI CTTGAG 1 cut(s) 215
BpuMI CCSGG 2 cut(s) 120, 160
BsaJI CCNNGG 1 cut(s) 482
Bsc4I CCNNNNNNNGG 1 cut(s) 458
Bse1I ACTGG 1 cut(s) 65
Bse3DI GCAATG 1 cut(s) 322
BseBI CCWGG 1 cut(s) 288
BseDI CCNNGG 1 cut(s) 482
BseGI GGATG 2 cut(s) 113, 142
BseLI CCNNNNNNNGG 1 cut(s) 458
BseMI GCAATG 1 cut(s) 322
BseNI ACTGG 1 cut(s) 65
BsePI GCGCGC 1 cut(s) 530
BseRI GAGGAG 2 cut(s) 375, 526
BseXI GCAGC 2 cut(s) 61, 142
BsgI GTGCAG 3 cut(s) 398, 420, 426
Bsh1236I CGCG 1 cut(s) 532
Bsh1285I CGRYCG 1 cut(s) 375
BshFI GGCC 3 cut(s) 50, 286, 291
BsiEI CGRYCG 1 cut(s) 375
BsiHKAI GWGCWC 1 cut(s) 316
BsiSI CCGG 2 cut(s) 120, 159
BslI CCNNNNNNNGG 1 cut(s) 458
BsmAI GTCTC 2 cut(s) 334, 387
BsnI GGCC 3 cut(s) 50, 286, 291
Bsp1286I GDGCHC 1 cut(s) 316
Bsp143I GATC 3 cut(s) 319, 361, 372
BspACI CCGC 2 cut(s) 309, 399
BspANI GGCC 3 cut(s) 50, 286, 291
BspFNI CGCG 1 cut(s) 532
BspHI TCATGA 1 cut(s) 270
BspMI ACCTGC 1 cut(s) 448
BsrDI GCAATG 1 cut(s) 322
BsrI ACTGG 1 cut(s) 65
BssECI CCNNGG 1 cut(s) 482
BssHII GCGCGC 1 cut(s) 530
BssMI GATC 3 cut(s) 319, 361, 372
BssT1I CCWWGG 1 cut(s) 482
Bst2UI CCWGG 1 cut(s) 288
Bst4CI ACNGT 1 cut(s) 40
Bst6I CTCTTC 2 cut(s) 26, 158
BstC8I GCNNGC 1 cut(s) 532
BstDEI CTNAG 2 cut(s) 457, 538
BstF5I GGATG 2 cut(s) 113, 142
BstFNI CGCG 1 cut(s) 532
BstHHI GCGC 2 cut(s) 532, 534
BstKTI GATC 3 cut(s) 322, 364, 375
BstMAI GTCTC 2 cut(s) 334, 387
BstMBI GATC 3 cut(s) 319, 361, 372
BstMCI CGRYCG 1 cut(s) 375
BstMWI GCNNNNNNNGC 4 cut(s) 231, 283, 496, 527
BstNI CCWGG 1 cut(s) 288
BstSCI CCNGG 3 cut(s) 118, 158, 286
BstUI CGCG 1 cut(s) 532
BstV1I GCAGC 2 cut(s) 61, 142
BstV2I GAAGAC 1 cut(s) 34
BsuRI GGCC 3 cut(s) 50, 286, 291
BtsCI GGATG 2 cut(s) 113, 142
BtsI GCAGTG 1 cut(s) 220
BtsIMutI CAGTG 1 cut(s) 220
BveI ACCTGC 1 cut(s) 448
Cac8I GCNNGC 1 cut(s) 532
CciI TCATGA 1 cut(s) 270
CfoI GCGC 2 cut(s) 532, 534
Cfr13I GGNCC 1 cut(s) 290
Csp6I GTAC 1 cut(s) 142
CviAII CATG 3 cut(s) 26, 173, 271
CviQI GTAC 1 cut(s) 142
DdeI CTNAG 2 cut(s) 457, 538
DpnI GATC 3 cut(s) 321, 363, 374
DpnII GATC 3 cut(s) 319, 361, 372
DriI GACNNNNNGTC 1 cut(s) 347
EaeI YGGCCR 1 cut(s) 48
Eam1104I CTCTTC 2 cut(s) 26, 158
Eam1105I GACNNNNNGTC 1 cut(s) 347
EarI CTCTTC 2 cut(s) 26, 158
Ecl136II GAGCTC 1 cut(s) 314
Eco130I CCWWGG 1 cut(s) 482
Eco147I AGGCCT 1 cut(s) 286
Eco24I GRGCYC 1 cut(s) 316
Eco53kI GAGCTC 1 cut(s) 314
EcoICRI GAGCTC 1 cut(s) 314
EcoRII CCWGG 1 cut(s) 286
EcoT14I CCWWGG 1 cut(s) 482
EcoT38I GRGCYC 1 cut(s) 316
ErhI CCWWGG 1 cut(s) 482
FaeI CATG 3 cut(s) 29, 176, 274
FaiI YATR 4 cut(s) 27, 174, 272, 497
FatI CATG 3 cut(s) 25, 172, 270
FbaI TGATCA 1 cut(s) 319
Fnu4HI GCNGC 3 cut(s) 75, 156, 399
FokI GGATG 2 cut(s) 120, 149
FriOI GRGCYC 1 cut(s) 316
Fsp4HI GCNGC 3 cut(s) 75, 156, 399
FspBI CTAG 1 cut(s) 547
GlaI GCGC 2 cut(s) 531, 533
GluI GCNGC 3 cut(s) 75, 156, 399
HaeIII GGCC 3 cut(s) 50, 286, 291
HapII CCGG 2 cut(s) 120, 159
HhaI GCGC 2 cut(s) 532, 534
Hin1II CATG 3 cut(s) 29, 176, 274
Hin6I GCGC 2 cut(s) 530, 532
HinP1I GCGC 2 cut(s) 530, 532
HincII GTYRAC 1 cut(s) 202
HindII GTYRAC 1 cut(s) 202
HindIII AAGCTT 1 cut(s) 454
HinfI GANTC 2 cut(s) 182, 428
HpaII CCGG 2 cut(s) 120, 159
HphI GGTGA 2 cut(s) 107, 367
Hpy166II GTNNAC 2 cut(s) 142, 202
Hpy188I TCNGA 2 cut(s) 89, 179
Hpy188III TCNNGA 3 cut(s) 194, 271, 317
Hpy8I GTNNAC 2 cut(s) 142, 202
HpyCH4III ACNGT 1 cut(s) 40
HpyCH4V TGCA 6 cut(s) 77, 225, 327, 379, 437, 443
HpyF10VI GCNNNNNNNGC 4 cut(s) 231, 283, 496, 527
HpyF3I CTNAG 2 cut(s) 457, 538
Hsp92II CATG 3 cut(s) 29, 176, 274
HspAI GCGC 2 cut(s) 530, 532
Ksp22I TGATCA 1 cut(s) 319
Kzo9I GATC 3 cut(s) 319, 361, 372
LmnI GCTCC 3 cut(s) 311, 319, 539
Lsp1109I GCAGC 2 cut(s) 61, 142
LweI GCATC 1 cut(s) 276
MaeI CTAG 1 cut(s) 547
MaeIII GTNAC 1 cut(s) 52
MalI GATC 3 cut(s) 321, 363, 374
MboI GATC 3 cut(s) 319, 361, 372
MboII GAAGA 4 cut(s) 13, 34, 175, 356
MhlI GDGCHC 1 cut(s) 316
MluCI AATT 2 cut(s) 58, 462
MlyI GAGTC 1 cut(s) 422
MmeI TCCRAC 1 cut(s) 157
MnlI CCTC 7 cut(s) 105, 190, 346, 396, 457, 510, 547
MslI CAYNNNNRTG 1 cut(s) 137
MspA1I CMGCKG 1 cut(s) 309
MspI CCGG 2 cut(s) 120, 159
MspR9I CCNGG 3 cut(s) 120, 160, 288
MvaI CCWGG 1 cut(s) 288
MvnI CGCG 1 cut(s) 532
MwoI GCNNNNNNNGC 4 cut(s) 231, 283, 496, 527
NciI CCSGG 2 cut(s) 120, 160
NdeII GATC 3 cut(s) 319, 361, 372
NlaIII CATG 3 cut(s) 29, 176, 274
NmuCI GTSAC 1 cut(s) 52
OliI CACNNNNGTG 1 cut(s) 137
PagI TCATGA 1 cut(s) 270
PaqCI CACCTGC 1 cut(s) 448
PauI GCGCGC 1 cut(s) 530
PceI AGGCCT 1 cut(s) 286
PfeI GAWTC 1 cut(s) 182
PkrI GCNGC 3 cut(s) 76, 157, 400
Ple19I CGATCG 1 cut(s) 375
PleI GAGTC 1 cut(s) 422
PpsI GAGTC 1 cut(s) 422
Psp124BI GAGCTC 1 cut(s) 316
Psp6I CCWGG 1 cut(s) 286
PspGI CCWGG 1 cut(s) 286
PspPI GGNCC 1 cut(s) 290
PteI GCGCGC 1 cut(s) 530
PvuI CGATCG 1 cut(s) 375
RsaI GTAC 1 cut(s) 143
RsaNI GTAC 1 cut(s) 142
RseI CAYNNNNRTG 1 cut(s) 137
SacI GAGCTC 1 cut(s) 316
SatI GCNGC 3 cut(s) 75, 156, 399
Sau3AI GATC 3 cut(s) 319, 361, 372
Sau96I GGNCC 1 cut(s) 290
SchI GAGTC 1 cut(s) 422
ScrFI CCNGG 3 cut(s) 120, 160, 288
SduI GDGCHC 1 cut(s) 316
SetI ASST 8 cut(s) 76, 201, 279, 316, 357, 409, 442, 458
SfaNI GCATC 1 cut(s) 276
SmiMI CAYNNNNRTG 1 cut(s) 137
SmlI CTYRAG 1 cut(s) 194
SmoI CTYRAG 1 cut(s) 194
Sse9I AATT 2 cut(s) 58, 462
SseBI AGGCCT 1 cut(s) 286
SsiI CCGC 2 cut(s) 309, 399
SspI AATATT 1 cut(s) 416
SspMI CTAG 1 cut(s) 547
SstI GAGCTC 1 cut(s) 316
StuI AGGCCT 1 cut(s) 286
StyD4I CCNGG 3 cut(s) 118, 158, 286
StyI CCWWGG 1 cut(s) 482
TaaI ACNGT 1 cut(s) 40
TaqI TCGA 3 cut(s) 246, 279, 351
TasI AATT 2 cut(s) 58, 462
TatI WGTACW 1 cut(s) 141
TauI GCSGC 1 cut(s) 401
TfiI GAWTC 1 cut(s) 182
TscAI CASTG 1 cut(s) 227
TseFI GTSAC 1 cut(s) 52
TseI GCWGC 2 cut(s) 74, 155
Tsp45I GTSAC 1 cut(s) 52
TspDTI ATGAA 3 cut(s) 161, 287, 292
TspGWI ACGGA 1 cut(s) 149
TspRI CASTG 1 cut(s) 227
XspI CTAG 1 cut(s) 547
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.