pycom09g16330

Dihydroorotase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0010110
Physical Location & Seq
Forward (+)
16576185 .. 16580274
4090 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1134 bp
ATGATAAAGAGTTGGGTTTTGCCTTGCAAGGCGTTGAAGTTTCCCTCATCAAATTTCGAAGGATCCAACAAGTTTCGGTGCAGAGGAGCCAAGATGGAGCTCACTATAACGCGCCCTGATGATTGGCACCTTCACCTTCGTGATGGTGAACTTCTGCAGGCTGTAGTCCCGCACAGTGCAAGTCATTTTGGAAGAGCAATTGTGATGCCTAATCTGAAACCTCCCATCACCACCACGGCTGCAGCTGTGGCTTATCGAGAATCCATCTTGAAAGCAATGCCTGCCGGTAGTGACTTCACACCACTTATGACCCTTTATTTGACTGATACCACAGAACCTAACGAGATCAAGCTCGCAAGAAGAAGTGGGGTTGTTTATGCTGTGAAGTTGTACCCTGCTGGTGCCACAACAAACTCTCAAGATGGGGTTACAGATCTCTTTGGAAGATGCCTACCTGTCCTTGAGGAGATGGCTGAACAGAATATACCTCTGCTGGTTCATGGGGAGGTTACAGATCCTGATATTGATGTATTTGATCGTGAAGGGGTCTTCATTGACACTGTTTTACGACCATTAATCCAAAGGCTTCCACAGCTAAAGGTCGTGATGGAGCATATCACTACCATGGATGCAGTTAAGTTTGTAGAGTCTTGTGAAGAAGGTTCTGTAGCTGCAACTGTTACTCCACAGCATCTTCTTCTAAATAGAAACTCTCTTTTCCAAGGAGGATTGCAGCCTCATAATTACTGTTTACCTGTACTCAAGAGAGAAATCCACAGACAGGCAATTGTGTCTGCTGTTACTAGTGGAAGTAAAAGATTTTTCATTGGAACTGATAGTGCTCCTCATGGGAGACGTAGAAAAGAAAGTTCTTGTGGATGTGCTGGTATATACAGTGCCCCTGTTGCTTTATCATTATACGCCAAGGTCTTTGAAGAGGCAGGTGCTATTGACAAGCTAGAAGCCTTTTTAAGCTTCAATGGCCCAGACTTCTATGGGCTTCCTCGAAACACGTCAAAGATAAAGCTGATCAAGAATTCATTCAACGTACCGGAGTGCTTCTCATTTCCGTTTGGAGACATTGTTCCGATGTTTGCTGGAGAAACACTTGAATGGCAGTCATCCATCAACTGA

Protein Analysis

378

Amino Acids

41.64

Weight (kDa)

6.8

Isoelectric Point (pI)

46.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Amidohydro_1 PF01979 41 - 342 2.2e-26 Amidohydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0010110)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G22930 AT4G22930
fragaria_vesca FvH4_6g26410 FvH4_6g26410 FvH4_6g26410 FvH4_6g26410
malus_domestica MD09G1233100.v1.1
prunus_persica Prupe.3G131200_v2.0.a1
pyrus_communis pycom09g16330
rosa_chinensis RchiOBHm_Chr2g0127911
rosa_laevigata RLG00000018930
rosa_multiflora Rmu_ssc0000093.1_g000008
rosa_roxburghii Rroxscaffold_2G00116850
rosa_rugosa Rorug02G0270300 Rorug02G0270300 Rorug02G0270400
rosa_samantha Rh2AG322800 Rh2BG331700 Rh2CG310800 Rh2DG350300
rosa_wichuraiana Rw2G026180

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 932
Acc36I ACCTGC 1 cut(s) 932
AccB1I GGYRCC 2 cut(s) 126, 401
AccII CGCG 1 cut(s) 112
AciI CCGC 1 cut(s) 170
AclWI GGATC 3 cut(s) 57, 70, 509
AcsI RAATTY 2 cut(s) 52, 1036
AfaI GTAC 3 cut(s) 392, 759, 1050
AfiI CCNNNNNNNGG 1 cut(s) 781
AflIII ACRYGT 1 cut(s) 1011
AgsI TTSAA 6 cut(s) 37, 271, 935, 979, 1045, 1112
AhlI ACTAGT 1 cut(s) 803
AjiI CACGTC 1 cut(s) 1014
AleI CACNNNNGTG 1 cut(s) 138
AloI GAACNNNNNNTCC 2 cut(s) 1068, 1100
AluBI AGCT 8 cut(s) 100, 245, 352, 595, 671, 958, 975, 1027
AluI AGCT 8 cut(s) 100, 245, 352, 595, 671, 958, 975, 1027
Alw21I GWGCWC 2 cut(s) 102, 844
Alw26I GTCTC 2 cut(s) 847, 1071
AlwI GGATC 3 cut(s) 57, 70, 509
AlwNI CAGNNNCTG 1 cut(s) 518
AoxI GGCC 1 cut(s) 982
ApeKI GCWGC 4 cut(s) 239, 242, 671, 733
ApoI RAATTY 2 cut(s) 52, 1036
ArsI GACNNNNNNTTYG 2 cut(s) 301, 333
AseI ATTAAT 1 cut(s) 575
Asp700I GAANNNNTTC 1 cut(s) 1040
AspLEI GCGC 1 cut(s) 114
AspS9I GGNCC 1 cut(s) 983
AsuHPI GGTGA 3 cut(s) 125, 158, 220
AsuII TTCGAA 1 cut(s) 57
BaeGI GKGCMC 1 cut(s) 901
BamHI GGATCC 1 cut(s) 62
BanI GGYRCC 2 cut(s) 126, 401
BanII GRGCYC 1 cut(s) 102
BbsI GAAGAC 1 cut(s) 541
Bbv12I GWGCWC 2 cut(s) 102, 844
BbvI GCAGC 4 cut(s) 226, 254, 658, 745
BccI CCATC 8 cut(s) 88, 137, 233, 272, 416, 463, 601, 1133
BceAI ACGGC 1 cut(s) 252
BclI TGATCA 1 cut(s) 1029
BcoDI GTCTC 2 cut(s) 847, 1071
BcuI ACTAGT 1 cut(s) 803
BfaI CTAG 2 cut(s) 804, 959
BfmI CTRYAG 4 cut(s) 155, 162, 240, 666
BfuAI ACCTGC 1 cut(s) 932
BglII AGATCT 1 cut(s) 433
BisI GCNGC 4 cut(s) 240, 243, 672, 734
BlsI GCNGC 4 cut(s) 241, 244, 673, 735
BmgBI CACGTC 1 cut(s) 1014
BmgT120I GGNCC 1 cut(s) 983
BmiI GGNNCC 4 cut(s) 64, 88, 128, 403
BmsI GCATC 4 cut(s) 195, 437, 619, 700
BpiI GAAGAC 1 cut(s) 541
BplI GAGNNNNNCTC 2 cut(s) 1046, 1078
BpmI CTGGAG 1 cut(s) 1119
Bpu14I TTCGAA 1 cut(s) 57
BpuEI CTTGAG 3 cut(s) 402, 482, 746
BsaJI CCNNGG 4 cut(s) 234, 624, 721, 924
BsaWI WCCGGW 1 cut(s) 1051
BsaXI ACNNNNNCTCC 4 cut(s) 667, 697, 1068, 1098
Bsc4I CCNNNNNNNGG 1 cut(s) 781
Bse118I RCCGGY 1 cut(s) 284
Bse3DI GCAATG 1 cut(s) 282
BseDI CCNNGG 4 cut(s) 234, 624, 721, 924
BseGI GGATG 3 cut(s) 634, 884, 1121
BseLI CCNNNNNNNGG 1 cut(s) 781
BseMI GCAATG 1 cut(s) 282
BseRI GAGGAG 3 cut(s) 99, 479, 834
BseSI GKGCMC 1 cut(s) 901
BseXI GCAGC 4 cut(s) 226, 254, 658, 745
BsgI GTGCAG 1 cut(s) 100
Bsh1236I CGCG 1 cut(s) 112
BshFI GGCC 1 cut(s) 984
BshNI GGYRCC 2 cut(s) 126, 401
BsiHKAI GWGCWC 2 cut(s) 102, 844
BsiSI CCGG 2 cut(s) 285, 1052
BslFI GGGAC 1 cut(s) 152
BslI CCNNNNNNNGG 1 cut(s) 781
BsmAI GTCTC 2 cut(s) 847, 1071
BsmBI CGTCTC 1 cut(s) 847
BsmFI GGGAC 1 cut(s) 152
BsnI GGCC 1 cut(s) 984
Bsp119I TTCGAA 1 cut(s) 57
Bsp1286I GDGCHC 3 cut(s) 102, 844, 901
Bsp143I GATC 6 cut(s) 62, 345, 433, 514, 535, 1029
Bsp19I CCATGG 1 cut(s) 624
BspACI CCGC 1 cut(s) 170
BspANI GGCC 1 cut(s) 984
BspFNI CGCG 1 cut(s) 112
BspLI GGNNCC 4 cut(s) 64, 88, 128, 403
BspMAI CTGCAG 2 cut(s) 159, 244
BspMI ACCTGC 1 cut(s) 932
BspPI GGATC 3 cut(s) 57, 70, 509
BspQI GCTCTTC 1 cut(s) 187
BspT104I TTCGAA 1 cut(s) 57
BspT107I GGYRCC 2 cut(s) 126, 401
BsrDI GCAATG 1 cut(s) 282
BsrFI RCCGGY 1 cut(s) 284
BssAI RCCGGY 1 cut(s) 284
BssECI CCNNGG 4 cut(s) 234, 624, 721, 924
BssMI GATC 6 cut(s) 62, 345, 433, 514, 535, 1029
BssT1I CCWWGG 3 cut(s) 624, 721, 924
Bst4CI ACNGT 5 cut(s) 176, 562, 679, 749, 896
Bst6I CTCTTC 2 cut(s) 187, 930
BstAPI GCANNNNNTGC 1 cut(s) 281
BstBI TTCGAA 1 cut(s) 57
BstC8I GCNNGC 3 cut(s) 159, 282, 354
BstDSI CCRYGG 2 cut(s) 234, 624
BstF5I GGATG 3 cut(s) 634, 884, 1121
BstFNI CGCG 1 cut(s) 112
BstHHI GCGC 1 cut(s) 114
BstKTI GATC 6 cut(s) 65, 348, 436, 517, 538, 1032
BstMAI GTCTC 2 cut(s) 847, 1071
BstMBI GATC 6 cut(s) 62, 345, 433, 514, 535, 1029
BstMWI GCNNNNNNNGC 5 cut(s) 248, 281, 592, 905, 981
BstSFI CTRYAG 4 cut(s) 155, 162, 240, 666
BstSLI GKGCMC 1 cut(s) 901
BstUI CGCG 1 cut(s) 112
BstV1I GCAGC 4 cut(s) 226, 254, 658, 745
BstV2I GAAGAC 1 cut(s) 541
BstX2I RGATCY 3 cut(s) 62, 433, 514
BstYI RGATCY 3 cut(s) 62, 433, 514
BsuRI GGCC 1 cut(s) 984
BtgI CCRYGG 2 cut(s) 234, 624
BtrI CACGTC 1 cut(s) 1014
BtsCI GGATG 3 cut(s) 634, 884, 1121
BtsIMutI CAGTG 3 cut(s) 181, 558, 901
BveI ACCTGC 1 cut(s) 932
Cac8I GCNNGC 3 cut(s) 159, 282, 354
CaiI CAGNNNCTG 1 cut(s) 518
CfoI GCGC 1 cut(s) 114
Cfr10I RCCGGY 1 cut(s) 284
Cfr13I GGNCC 1 cut(s) 983
Csp6I GTAC 3 cut(s) 391, 758, 1049
CviAII CATG 3 cut(s) 500, 625, 848
CviQI GTAC 3 cut(s) 391, 758, 1049
DpnI GATC 6 cut(s) 64, 347, 435, 516, 537, 1031
DpnII GATC 6 cut(s) 62, 345, 433, 514, 535, 1029
Eam1104I CTCTTC 2 cut(s) 187, 930
EarI CTCTTC 2 cut(s) 187, 930
Ecl136II GAGCTC 1 cut(s) 100
Eco130I CCWWGG 3 cut(s) 624, 721, 924
Eco24I GRGCYC 1 cut(s) 102
Eco53kI GAGCTC 1 cut(s) 100
EcoICRI GAGCTC 1 cut(s) 100
EcoRI GAATTC 1 cut(s) 1036
EcoT14I CCWWGG 3 cut(s) 624, 721, 924
EcoT38I GRGCYC 1 cut(s) 102
ErhI CCWWGG 3 cut(s) 624, 721, 924
Esp3I CGTCTC 1 cut(s) 847
FaeI CATG 3 cut(s) 503, 628, 851
FaqI GGGAC 1 cut(s) 152
FatI CATG 3 cut(s) 499, 624, 847
FauI CCCGC 1 cut(s) 177
FbaI TGATCA 1 cut(s) 1029
Fnu4HI GCNGC 4 cut(s) 240, 243, 672, 734
FokI GGATG 3 cut(s) 641, 891, 1108
FriOI GRGCYC 1 cut(s) 102
Fsp4HI GCNGC 4 cut(s) 240, 243, 672, 734
FspBI CTAG 2 cut(s) 804, 959
GlaI GCGC 1 cut(s) 113
GluI GCNGC 4 cut(s) 240, 243, 672, 734
GsuI CTGGAG 1 cut(s) 1119
HaeIII GGCC 1 cut(s) 984
HapII CCGG 2 cut(s) 285, 1052
HhaI GCGC 1 cut(s) 114
Hin1II CATG 3 cut(s) 503, 628, 851
Hin6I GCGC 1 cut(s) 112
HinP1I GCGC 1 cut(s) 112
HindIII AAGCTT 1 cut(s) 973
HinfI GANTC 2 cut(s) 260, 647
HpaII CCGG 2 cut(s) 285, 1052
HphI GGTGA 3 cut(s) 125, 158, 220
Hpy166II GTNNAC 2 cut(s) 149, 752
Hpy188I TCNGA 2 cut(s) 216, 1089
Hpy188III TCNNGA 9 cut(s) 140, 257, 268, 419, 518, 539, 604, 763, 1033
Hpy8I GTNNAC 2 cut(s) 149, 752
HpyAV CCTTC 5 cut(s) 53, 140, 146, 536, 653
HpyCH4III ACNGT 5 cut(s) 176, 562, 679, 749, 896
HpyCH4IV ACGT 3 cut(s) 856, 1013, 1047
HpyCH4V TGCA 8 cut(s) 27, 81, 157, 179, 242, 632, 674, 733
HpyF10VI GCNNNNNNNGC 5 cut(s) 248, 281, 592, 905, 981
HpySE526I ACGT 3 cut(s) 856, 1013, 1047
Hsp92II CATG 3 cut(s) 503, 628, 851
HspAI GCGC 1 cut(s) 112
Ksp22I TGATCA 1 cut(s) 1029
Kzo9I GATC 6 cut(s) 62, 345, 433, 514, 535, 1029
LguI GCTCTTC 1 cut(s) 187
LmnI GCTCC 4 cut(s) 86, 97, 610, 847
Lsp1109I GCAGC 4 cut(s) 226, 254, 658, 745
LweI GCATC 4 cut(s) 195, 437, 619, 700
MaeI CTAG 2 cut(s) 804, 959
MaeII ACGT 3 cut(s) 856, 1013, 1047
MaeIII GTNAC 5 cut(s) 290, 427, 508, 679, 799
MalI GATC 6 cut(s) 64, 347, 435, 516, 537, 1031
MboI GATC 6 cut(s) 62, 345, 433, 514, 535, 1029
MboII GAAGA 8 cut(s) 204, 372, 456, 541, 668, 686, 689, 947
MfeI CAATTG 2 cut(s) 198, 786
MflI RGATCY 3 cut(s) 62, 433, 514
MhlI GDGCHC 3 cut(s) 102, 844, 901
MluCI AATT 5 cut(s) 52, 198, 742, 786, 1036
MlyI GAGTC 1 cut(s) 656
MmeI TCCRAC 1 cut(s) 90
MroXI GAANNNNTTC 1 cut(s) 1040
MseI TTAA 3 cut(s) 575, 636, 971
MslI CAYNNNNRTG 3 cut(s) 138, 623, 1111
MspA1I CMGCKG 1 cut(s) 245
MspI CCGG 2 cut(s) 285, 1052
MunI CAATTG 2 cut(s) 198, 786
MvnI CGCG 1 cut(s) 112
MwoI GCNNNNNNNGC 5 cut(s) 248, 281, 592, 905, 981
NcoI CCATGG 1 cut(s) 624
NdeII GATC 6 cut(s) 62, 345, 433, 514, 535, 1029
NlaIII CATG 3 cut(s) 503, 628, 851
NlaIV GGNNCC 4 cut(s) 64, 88, 128, 403
NmuCI GTSAC 1 cut(s) 290
NspV TTCGAA 1 cut(s) 57
OliI CACNNNNGTG 1 cut(s) 138
PaqCI CACCTGC 1 cut(s) 932
PciSI GCTCTTC 1 cut(s) 187
PdmI GAANNNNTTC 1 cut(s) 1040
PfeI GAWTC 1 cut(s) 260
PkrI GCNGC 4 cut(s) 241, 244, 673, 735
PleI GAGTC 1 cut(s) 655
PpsI GAGTC 1 cut(s) 655
PshBI ATTAAT 1 cut(s) 575
Psp124BI GAGCTC 1 cut(s) 102
PspN4I GGNNCC 4 cut(s) 64, 88, 128, 403
PspPI GGNCC 1 cut(s) 983
PsrI GAACNNNNNNTAC 2 cut(s) 468, 500
PstI CTGCAG 2 cut(s) 159, 244
PstNI CAGNNNCTG 1 cut(s) 518
PsuI RGATCY 3 cut(s) 62, 433, 514
PvuII CAGCTG 1 cut(s) 245
RsaI GTAC 3 cut(s) 392, 759, 1050
RsaNI GTAC 3 cut(s) 391, 758, 1049
RseI CAYNNNNRTG 3 cut(s) 138, 623, 1111
SacI GAGCTC 1 cut(s) 102
SapI GCTCTTC 1 cut(s) 187
SaqAI TTAA 3 cut(s) 575, 636, 971
SatI GCNGC 4 cut(s) 240, 243, 672, 734
Sau3AI GATC 6 cut(s) 62, 345, 433, 514, 535, 1029
Sau96I GGNCC 1 cut(s) 983
SchI GAGTC 1 cut(s) 656
SduI GDGCHC 3 cut(s) 102, 844, 901
SfaNI GCATC 4 cut(s) 195, 437, 619, 700
SfcI CTRYAG 4 cut(s) 155, 162, 240, 666
SfuI TTCGAA 1 cut(s) 57
SmiMI CAYNNNNRTG 3 cut(s) 138, 623, 1111
SmlI CTYRAG 3 cut(s) 417, 461, 761
SmoI CTYRAG 3 cut(s) 417, 461, 761
SpeI ACTAGT 1 cut(s) 803
Sse9I AATT 5 cut(s) 52, 198, 742, 786, 1036
SsiI CCGC 1 cut(s) 170
SspMI CTAG 2 cut(s) 804, 959
SstI GAGCTC 1 cut(s) 102
StyI CCWWGG 3 cut(s) 624, 721, 924
TaaI ACNGT 5 cut(s) 176, 562, 679, 749, 896
TaiI ACGT 3 cut(s) 859, 1016, 1050
TaqI TCGA 3 cut(s) 57, 256, 1006
TasI AATT 5 cut(s) 52, 198, 742, 786, 1036
TatI WGTACW 1 cut(s) 757
TfiI GAWTC 1 cut(s) 260
Tru1I TTAA 3 cut(s) 575, 636, 971
Tru9I TTAA 3 cut(s) 575, 636, 971
TscAI CASTG 3 cut(s) 181, 565, 901
TseFI GTSAC 1 cut(s) 290
TseI GCWGC 4 cut(s) 239, 242, 671, 733
Tsp45I GTSAC 1 cut(s) 290
TspDTI ATGAA 4 cut(s) 488, 541, 814, 1029
TspGWI ACGGA 1 cut(s) 1059
TspRI CASTG 3 cut(s) 181, 565, 901
VspI ATTAAT 1 cut(s) 575
XapI RAATTY 2 cut(s) 52, 1036
XmnI GAANNNNTTC 1 cut(s) 1040
XspI CTAG 2 cut(s) 804, 959
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.