pycom09g17370

S-adenosyl-L-methionine-dependent protein-lysine N- methyltransferase that methylates elongation factor 1-alpha

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0014371
Physical Location & Seq
Forward (+)
17864680 .. 17869166
4487 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 990 bp
ATGGCTGGATTCCGGTTGGGGTTAGATGACTCGGAGATATCGCAGCAGAGCCGAGCAGCACCGCCTGATCTCGTCTCCGACGACGACCGCTCCATCGCCGCCGACTCATGGTCCATCAAGAGTGACTACGGAAGCACTCTCGACGACGACCAGCGCCATGCCGATGCCGCCGAGGACCTTTCTGCTGCTAACCTTCGCGCCCCCTCCGAGTACAGTTCTGACAAGGAGGAACCAGATGCTGAAGGAGTGACATCAATGCTAGGTCGTCAGAGTTACTGGGATGCTGCATATGCCGATGAGTTGGCGAATTTTCGGGAACATGGTCATACCGGTGAAGTTTGGTTTGGAACTAATGTCATGGAAGTTGTCTCCTCTTGGACCAAAAGCTTATGCATTGATATTTCTCAAGGTCGCACACCAAATCATGATAATAATAAGTCTGATCCTGTGGAAAAAGGTGATAAGTATCTTGCAGGCTGGAGTGTACTTGACATCGGGACTGGCAATGGATTGCTTCTTCAAGCACTTTCCAAGCAGGGGTTTTCTGATTTAACAGGAACTGATTATAGTGAAGGGGCAATTGATCTTGCTCGCAGCCTTGCTGATCGTGATGGATTGCCAAACATTAATTTTTTGGATGTTCTAGAGACCAAGTTGGACAGGCAGTTCAAACTAGTTGTGGATAAAGGAACCTTGGATGCCATTGGATTGCATCCAGATGGTTCTATCAAAAGGGTAATGTATTGGGGTTCCGTGTCAAAATTGGTGGCTCCTGGTGGATTAGCAGTGATCACATCATGTAACAACACAAAGGATGAATTGGTTCAAGAAGTGGAAAGTTTCAATCAAAGAAGTGTCATCCAGGAACACGAAACAAGCGAAGATCCTCCTAAGTTTCAATACCTTAGTCATGTCCGCACATATCCGACATTCGCGTTTGGTGGATCTGTTGGATCGCGCGTTGCCACTGTGGCATTTCTCCGGAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_31

Protein Analysis

330

Amino Acids

35.92

Weight (kDa)

4.57

Isoelectric Point (pI)

32.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_31 PF13847 159 - 283 1.6e-13 Methyltransferase domain
Methyltransf_23 PF13489 161 - 283 2.7e-08 Methyltransferase domain
Methyltransf_25 PF13649 162 - 230 5.9e-09 Methyltransferase domain
Methyltransf_12 PF08242 163 - 261 4.9e-08 Methyltransferase domain
Methyltransf_11 PF08241 163 - 235 1.5e-06 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014371)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G66680
fragaria_vesca FvH4_1g29040 FvH4_1g29040 FvH4_1g29040
malus_domestica MD09G1258900.v1.1 MD17G1253400.v1.1
prunus_persica Prupe.3G150900_v2.0.a1
pyrus_communis pycom09g17370
rosa_chinensis RchiOBHm_Chr3g0493661
rosa_laevigata RLG00000022698
rosa_multiflora Rmu_sc0000568.1_g000014
rosa_roxburghii Rroxscaffold_6G00391390
rosa_rugosa Rorug03G0263300
rosa_samantha Rh3AG312200 Rh3BG348100 Rh3CG345800
rosa_wichuraiana Rw3G027390

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 90
AccII CGCG 4 cut(s) 198, 935, 958, 960
AccIII TCCGGA 1 cut(s) 981
AciI CCGC 5 cut(s) 62, 88, 99, 168, 916
AclWI GGATC 4 cut(s) 437, 878, 952, 961
AcsI RAATTY 1 cut(s) 307
AcuI CTGAAG 1 cut(s) 261
AfaI GTAC 2 cut(s) 212, 486
AfiI CCNNNNNNNGG 2 cut(s) 108, 537
AgeI ACCGGT 1 cut(s) 329
AgsI TTSAA 5 cut(s) 521, 670, 827, 844, 899
AhdI GACNNNNNGTC 1 cut(s) 109
AhlI ACTAGT 1 cut(s) 673
AjnI CCWGG 2 cut(s) 772, 861
AjuI GAANNNNNNNTTGG 2 cut(s) 327, 359
AloI GAACNNNNNNTCC 2 cut(s) 650, 682
AluBI AGCT 1 cut(s) 387
AluI AGCT 1 cut(s) 387
Alw26I GTCTC 3 cut(s) 79, 373, 641
AlwI GGATC 4 cut(s) 437, 878, 952, 961
AlwNI CAGNNNCTG 2 cut(s) 239, 560
Aor13HI TCCGGA 1 cut(s) 981
ApeKI GCWGC 5 cut(s) 43, 56, 185, 284, 594
ApoI RAATTY 1 cut(s) 307
AseI ATTAAT 1 cut(s) 627
AsiGI ACCGGT 1 cut(s) 329
Asp700I GAANNNNTTC 1 cut(s) 822
AspLEI GCGC 3 cut(s) 156, 200, 960
AspS9I GGNCC 3 cut(s) 111, 175, 378
AsuHPI GGTGA 2 cut(s) 344, 470
AvaII GGWCC 3 cut(s) 111, 175, 378
BbvI GCAGC 5 cut(s) 55, 68, 172, 271, 606
BccI CCATC 4 cut(s) 101, 122, 605, 713
BciT130I CCWGG 2 cut(s) 774, 863
BclI TGATCA 1 cut(s) 789
BcoDI GTCTC 3 cut(s) 79, 373, 641
BcuI ACTAGT 1 cut(s) 673
BfaI CTAG 3 cut(s) 260, 644, 674
BfoI RGCGCY 1 cut(s) 157
BglI GCCNNNNNGGC 1 cut(s) 971
BisI GCNGC 7 cut(s) 44, 57, 99, 168, 186, 285, 595
BlsI GCNGC 7 cut(s) 45, 58, 100, 169, 187, 286, 596
Bme1390I CCNGG 2 cut(s) 774, 863
Bme18I GGWCC 3 cut(s) 111, 175, 378
BmeRI GACNNNNNGTC 1 cut(s) 109
BmgT120I GGNCC 3 cut(s) 111, 175, 378
BmiI GGNNCC 4 cut(s) 231, 691, 751, 771
BmrFI CCNGG 2 cut(s) 774, 863
BmrI ACTGGG 1 cut(s) 286
BmsI GCATC 5 cut(s) 154, 226, 271, 688, 721
BmuI ACTGGG 1 cut(s) 286
BpmI CTGGAG 1 cut(s) 499
BpuEI CTTGAG 1 cut(s) 390
BsaBI GATNNNNATC 1 cut(s) 465
BsaI GGTCTC 1 cut(s) 641
BsaJI CCNNGG 2 cut(s) 171, 693
BsaWI WCCGGW 3 cut(s) 12, 329, 981
BsaXI ACNNNNNCTCC 2 cut(s) 74, 104
Bsc4I CCNNNNNNNGG 2 cut(s) 108, 537
Bse118I RCCGGY 1 cut(s) 329
Bse1I ACTGG 2 cut(s) 281, 505
Bse3DI GCAATG 1 cut(s) 511
Bse8I GATNNNNATC 1 cut(s) 465
BseAI TCCGGA 1 cut(s) 981
BseBI CCWGG 2 cut(s) 774, 863
BseDI CCNNGG 2 cut(s) 171, 693
BseGI GGATG 6 cut(s) 286, 643, 703, 712, 820, 858
BseJI GATNNNNATC 1 cut(s) 465
BseLI CCNNNNNNNGG 2 cut(s) 108, 537
BseMI GCAATG 1 cut(s) 511
BseNI ACTGG 2 cut(s) 281, 505
BseRI GAGGAG 1 cut(s) 361
BseXI GCAGC 5 cut(s) 55, 68, 172, 271, 606
Bsh1236I CGCG 4 cut(s) 198, 935, 958, 960
Bsh1285I CGRYCG 1 cut(s) 88
BshTI ACCGGT 1 cut(s) 329
BsiEI CGRYCG 1 cut(s) 88
BsiSI CCGG 3 cut(s) 13, 330, 982
BslFI GGGAC 1 cut(s) 511
BslI CCNNNNNNNGG 2 cut(s) 108, 537
BsmAI GTCTC 3 cut(s) 79, 373, 641
BsmBI CGTCTC 1 cut(s) 79
BsmFI GGGAC 1 cut(s) 511
Bso31I GGTCTC 1 cut(s) 641
Bsp13I TCCGGA 1 cut(s) 981
Bsp143I GATC 8 cut(s) 67, 442, 583, 604, 789, 883, 944, 953
BspACI CCGC 5 cut(s) 62, 88, 99, 168, 916
BspEI TCCGGA 1 cut(s) 981
BspFNI CGCG 4 cut(s) 198, 935, 958, 960
BspHI TCATGA 1 cut(s) 424
BspLI GGNNCC 4 cut(s) 231, 691, 751, 771
BspPI GGATC 4 cut(s) 437, 878, 952, 961
BspTNI GGTCTC 1 cut(s) 641
BsrBI CCGCTC 1 cut(s) 90
BsrDI GCAATG 1 cut(s) 511
BsrFI RCCGGY 1 cut(s) 329
BsrI ACTGG 2 cut(s) 281, 505
BssAI RCCGGY 1 cut(s) 329
BssECI CCNNGG 2 cut(s) 171, 693
BssMI GATC 8 cut(s) 67, 442, 583, 604, 789, 883, 944, 953
BssT1I CCWWGG 1 cut(s) 693
Bst2UI CCWGG 2 cut(s) 774, 863
Bst4CI ACNGT 2 cut(s) 215, 970
BstC8I GCNNGC 2 cut(s) 475, 592
BstDEI CTNAG 2 cut(s) 891, 905
BstF5I GGATG 6 cut(s) 286, 643, 703, 712, 820, 858
BstFNI CGCG 4 cut(s) 198, 935, 958, 960
BstH2I RGCGCY 1 cut(s) 157
BstHHI GCGC 3 cut(s) 156, 200, 960
BstKTI GATC 8 cut(s) 70, 445, 586, 607, 792, 886, 947, 956
BstMAI GTCTC 3 cut(s) 79, 373, 641
BstMBI GATC 8 cut(s) 67, 442, 583, 604, 789, 883, 944, 953
BstMCI CGRYCG 1 cut(s) 88
BstMWI GCNNNNNNNGC 3 cut(s) 167, 290, 971
BstNI CCWGG 2 cut(s) 774, 863
BstSCI CCNGG 2 cut(s) 772, 861
BstUI CGCG 4 cut(s) 198, 935, 958, 960
BstV1I GCAGC 5 cut(s) 55, 68, 172, 271, 606
BstX2I RGATCY 2 cut(s) 883, 944
BstYI RGATCY 2 cut(s) 883, 944
BtgZI GCGATG 1 cut(s) 79
BtsCI GGATG 6 cut(s) 286, 643, 703, 712, 820, 858
BtsI GCAGTG 1 cut(s) 792
BtsIMutI CAGTG 2 cut(s) 792, 966
Cac8I GCNNGC 2 cut(s) 475, 592
CaiI CAGNNNCTG 2 cut(s) 239, 560
CciI TCATGA 1 cut(s) 424
CfoI GCGC 3 cut(s) 156, 200, 960
Cfr10I RCCGGY 1 cut(s) 329
Cfr13I GGNCC 3 cut(s) 111, 175, 378
Csp6I GTAC 2 cut(s) 211, 485
CspAI ACCGGT 1 cut(s) 329
CspCI CAANNNNNGTGG 2 cut(s) 747, 782
CviAII CATG 7 cut(s) 108, 158, 320, 358, 425, 798, 911
CviJI RGCY 6 cut(s) 5, 51, 387, 477, 597, 770
CviKI_1 RGCY 6 cut(s) 5, 51, 387, 477, 597, 770
CviQI GTAC 2 cut(s) 211, 485
DdeI CTNAG 2 cut(s) 891, 905
DpnI GATC 8 cut(s) 69, 444, 585, 606, 791, 885, 946, 955
DpnII GATC 8 cut(s) 67, 442, 583, 604, 789, 883, 944, 953
DriI GACNNNNNGTC 1 cut(s) 109
Eam1105I GACNNNNNGTC 1 cut(s) 109
Eco130I CCWWGG 1 cut(s) 693
Eco31I GGTCTC 1 cut(s) 641
Eco32I GATATC 1 cut(s) 39
Eco47I GGWCC 3 cut(s) 111, 175, 378
Eco57I CTGAAG 1 cut(s) 261
EcoO109I RGGNCCY 1 cut(s) 175
EcoRII CCWGG 2 cut(s) 772, 861
EcoRV GATATC 1 cut(s) 39
EcoT14I CCWWGG 1 cut(s) 693
EcoT22I ATGCAT 1 cut(s) 395
ErhI CCWWGG 1 cut(s) 693
Esp3I CGTCTC 1 cut(s) 79
FaeI CATG 7 cut(s) 111, 161, 323, 361, 428, 801, 914
FaqI GGGAC 1 cut(s) 511
FatI CATG 7 cut(s) 107, 157, 319, 357, 424, 797, 910
FauNDI CATATG 1 cut(s) 289
FbaI TGATCA 1 cut(s) 789
Fnu4HI GCNGC 7 cut(s) 44, 57, 99, 168, 186, 285, 595
FokI GGATG 6 cut(s) 293, 650, 699, 710, 827, 845
Fsp4HI GCNGC 7 cut(s) 44, 57, 99, 168, 186, 285, 595
FspBI CTAG 3 cut(s) 260, 644, 674
GlaI GCGC 3 cut(s) 155, 199, 959
GluI GCNGC 7 cut(s) 44, 57, 99, 168, 186, 285, 595
GsuI CTGGAG 1 cut(s) 499
HaeII RGCGCY 1 cut(s) 157
HapII CCGG 3 cut(s) 13, 330, 982
HhaI GCGC 3 cut(s) 156, 200, 960
Hin1II CATG 7 cut(s) 111, 161, 323, 361, 428, 801, 914
Hin6I GCGC 3 cut(s) 154, 198, 958
HinP1I GCGC 3 cut(s) 154, 198, 958
HindIII AAGCTT 1 cut(s) 385
HinfI GANTC 3 cut(s) 9, 29, 104
HpaII CCGG 3 cut(s) 13, 330, 982
HphI GGTGA 2 cut(s) 344, 470
Hpy166II GTNNAC 1 cut(s) 485
Hpy188I TCNGA 8 cut(s) 34, 79, 208, 220, 270, 442, 547, 927
Hpy8I GTNNAC 1 cut(s) 485
Hpy99I CGWCG 4 cut(s) 83, 86, 146, 149
HpyAV CCTTC 3 cut(s) 203, 236, 566
HpyCH4III ACNGT 2 cut(s) 215, 970
HpyCH4V TGCA 4 cut(s) 287, 393, 473, 712
HpyF10VI GCNNNNNNNGC 3 cut(s) 167, 290, 971
HpyF3I CTNAG 2 cut(s) 891, 905
Hsp92II CATG 7 cut(s) 111, 161, 323, 361, 428, 801, 914
HspAI GCGC 3 cut(s) 154, 198, 958
Kpn2I TCCGGA 1 cut(s) 981
Ksp22I TGATCA 1 cut(s) 789
Kzo9I GATC 8 cut(s) 67, 442, 583, 604, 789, 883, 944, 953
LmnI GCTCC 2 cut(s) 95, 775
Lsp1109I GCAGC 5 cut(s) 55, 68, 172, 271, 606
LweI GCATC 5 cut(s) 154, 226, 271, 688, 721
MaeI CTAG 3 cut(s) 260, 644, 674
MaeIII GTNAC 4 cut(s) 122, 247, 272, 800
MalI GATC 8 cut(s) 69, 444, 585, 606, 791, 885, 946, 955
MbiI CCGCTC 1 cut(s) 90
MboI GATC 8 cut(s) 67, 442, 583, 604, 789, 883, 944, 953
MboII GAAGA 2 cut(s) 509, 893
MfeI CAATTG 1 cut(s) 579
MflI RGATCY 2 cut(s) 883, 944
MluCI AATT 5 cut(s) 307, 579, 628, 761, 818
MlyI GAGTC 2 cut(s) 23, 98
MmeI TCCRAC 4 cut(s) 102, 636, 931, 950
MnlI CCTC 5 cut(s) 166, 214, 220, 382, 897
Mph1103I ATGCAT 1 cut(s) 395
MroI TCCGGA 1 cut(s) 981
MroXI GAANNNNTTC 1 cut(s) 822
MseI TTAA 2 cut(s) 551, 627
MslI CAYNNNNRTG 3 cut(s) 162, 330, 717
MspI CCGG 3 cut(s) 13, 330, 982
MspR9I CCNGG 2 cut(s) 774, 863
MunI CAATTG 1 cut(s) 579
MvaI CCWGG 2 cut(s) 774, 863
MvnI CGCG 4 cut(s) 198, 935, 958, 960
MwoI GCNNNNNNNGC 3 cut(s) 167, 290, 971
NdeI CATATG 1 cut(s) 289
NdeII GATC 8 cut(s) 67, 442, 583, 604, 789, 883, 944, 953
NlaIII CATG 7 cut(s) 111, 161, 323, 361, 428, 801, 914
NlaIV GGNNCC 4 cut(s) 231, 691, 751, 771
NmeAIII GCCGAG 2 cut(s) 77, 196
NmuCI GTSAC 2 cut(s) 122, 247
NsiI ATGCAT 1 cut(s) 395
PagI TCATGA 1 cut(s) 424
PcsI WCGNNNNNNNCGW 2 cut(s) 78, 876
PdmI GAANNNNTTC 1 cut(s) 822
PfeI GAWTC 1 cut(s) 9
PfoI TCCNGGA 1 cut(s) 861
PinAI ACCGGT 1 cut(s) 329
PkrI GCNGC 7 cut(s) 45, 58, 100, 169, 187, 286, 596
PleI GAGTC 2 cut(s) 23, 98
PpsI GAGTC 2 cut(s) 23, 98
PpuMI RGGWCCY 1 cut(s) 175
PshBI ATTAAT 1 cut(s) 627
Psp5II RGGWCCY 1 cut(s) 175
Psp6I CCWGG 2 cut(s) 772, 861
PspGI CCWGG 2 cut(s) 772, 861
PspN4I GGNNCC 4 cut(s) 231, 691, 751, 771
PspPI GGNCC 3 cut(s) 111, 175, 378
PspPPI RGGWCCY 1 cut(s) 175
PstNI CAGNNNCTG 2 cut(s) 239, 560
PsuI RGATCY 2 cut(s) 883, 944
RsaI GTAC 2 cut(s) 212, 486
RsaNI GTAC 2 cut(s) 211, 485
RseI CAYNNNNRTG 3 cut(s) 162, 330, 717
SaqAI TTAA 2 cut(s) 551, 627
SatI GCNGC 7 cut(s) 44, 57, 99, 168, 186, 285, 595
Sau3AI GATC 8 cut(s) 67, 442, 583, 604, 789, 883, 944, 953
Sau96I GGNCC 3 cut(s) 111, 175, 378
SchI GAGTC 2 cut(s) 23, 98
ScrFI CCNGG 2 cut(s) 774, 863
SetI ASST 8 cut(s) 180, 195, 265, 389, 412, 460, 695, 906
SfaNI GCATC 5 cut(s) 154, 226, 271, 688, 721
SinI GGWCC 3 cut(s) 111, 175, 378
SmiMI CAYNNNNRTG 3 cut(s) 162, 330, 717
SmlI CTYRAG 1 cut(s) 405
SmoI CTYRAG 1 cut(s) 405
SpeI ACTAGT 1 cut(s) 673
Sse9I AATT 5 cut(s) 307, 579, 628, 761, 818
SsiI CCGC 5 cut(s) 62, 88, 99, 168, 916
SspMI CTAG 3 cut(s) 260, 644, 674
StyD4I CCNGG 2 cut(s) 772, 861
StyI CCWWGG 1 cut(s) 693
TaaI ACNGT 2 cut(s) 215, 970
TaqI TCGA 1 cut(s) 141
TasI AATT 5 cut(s) 307, 579, 628, 761, 818
TatI WGTACW 2 cut(s) 210, 484
TauI GCSGC 2 cut(s) 101, 170
TfiI GAWTC 1 cut(s) 9
Tru1I TTAA 2 cut(s) 551, 627
Tru9I TTAA 2 cut(s) 551, 627
TscAI CASTG 2 cut(s) 792, 973
TseFI GTSAC 2 cut(s) 122, 247
TseI GCWGC 5 cut(s) 43, 56, 185, 284, 594
Tsp45I GTSAC 2 cut(s) 122, 247
TspDTI ATGAA 1 cut(s) 831
TspGWI ACGGA 2 cut(s) 144, 742
TspRI CASTG 2 cut(s) 792, 973
VpaK11BI GGWCC 3 cut(s) 111, 175, 378
VspI ATTAAT 1 cut(s) 627
XapI RAATTY 1 cut(s) 307
XbaI TCTAGA 1 cut(s) 643
XmnI GAANNNNTTC 1 cut(s) 822
XspI CTAG 3 cut(s) 260, 644, 674
Zsp2I ATGCAT 1 cut(s) 395
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.