pycom09g19220

Ribosomal protein S21

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0010416
Physical Location & Seq
Reverse (-)
19951305 .. 19952385
1081 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 333 bp
ATGAATGCGGTAGCAAGGAGTGCGTGGAATGTGTTGAAACGGTCCAGTCAGGGCATGGAGTCAGTGAGTCAGTTGGGGTACCATCGTCATGAGCAATTGAGAGGGATTCGGGTGAAGGTGATGAACGGGAACCTGGAGTGGGCCCTGACGGTTATGCAGAGGAAGATGACGTCGAGTGGGATTGAGAGGATGATAAAGCGGGAGCAAACCCACCACCTCAAAAACTCTGAGAAACGAATCTTGGCTCGCAAGAATCTCGAGCAGAAGCTCCGGTCTCAAGACCTTGCTCGCAAGCTCAAGGCCGTCCTCCTCAAGAAAGTCAGAGGCCTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_S21

Protein Analysis

111

Amino Acids

12.81

Weight (kDa)

11.54

Isoelectric Point (pI)

49.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_S21 PF01165 38 - 91 4.8e-10 Ribosomal protein S21
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 173
Acc65I GGTACC 1 cut(s) 78
AccB1I GGYRCC 1 cut(s) 78
AciI CCGC 2 cut(s) 8, 199
AcyI GRCGYC 1 cut(s) 170
AfaI GTAC 1 cut(s) 80
AfiI CCNNNNNNNGG 1 cut(s) 139
AgsI TTSAA 1 cut(s) 37
AjnI CCWGG 1 cut(s) 132
AjuI GAANNNNNNNTTGG 2 cut(s) 224, 256
AluBI AGCT 2 cut(s) 268, 295
AluI AGCT 2 cut(s) 268, 295
Alw26I GTCTC 1 cut(s) 279
Ama87I CYCGRG 1 cut(s) 257
AoxI GGCC 3 cut(s) 141, 300, 325
ApaI GGGCCC 1 cut(s) 145
Asp718I GGTACC 1 cut(s) 78
AspS9I GGNCC 3 cut(s) 42, 141, 142
AsuHPI GGTGA 2 cut(s) 124, 130
AvaI CYCGRG 1 cut(s) 257
AvaII GGWCC 1 cut(s) 42
BaeGI GKGCMC 1 cut(s) 145
BaeI ACNNNNGTAYC 2 cut(s) 70, 103
BanI GGYRCC 1 cut(s) 78
BanII GRGCYC 1 cut(s) 145
BccI CCATC 1 cut(s) 90
BceAI ACGGC 1 cut(s) 287
BcgI CGANNNNNNTGC 2 cut(s) 238, 272
BciT130I CCWGG 1 cut(s) 134
BcoDI GTCTC 1 cut(s) 279
Bme1390I CCNGG 1 cut(s) 134
Bme18I GGWCC 1 cut(s) 42
BmeT110I CYCGRG 1 cut(s) 257
BmgT120I GGNCC 3 cut(s) 42, 141, 142
BmiI GGNNCC 3 cut(s) 80, 131, 143
BmrFI CCNGG 1 cut(s) 134
BpmI CTGGAG 1 cut(s) 155
BpuEI CTTGAG 3 cut(s) 261, 281, 296
BsaHI GRCGYC 1 cut(s) 170
BsaI GGTCTC 1 cut(s) 279
BsaWI WCCGGW 1 cut(s) 270
Bsc4I CCNNNNNNNGG 1 cut(s) 139
Bse1I ACTGG 1 cut(s) 45
BseBI CCWGG 1 cut(s) 134
BseGI GGATG 1 cut(s) 195
BseLI CCNNNNNNNGG 1 cut(s) 139
BseMII CTCAG 1 cut(s) 219
BseNI ACTGG 1 cut(s) 45
BseRI GAGGAG 1 cut(s) 299
BseSI GKGCMC 1 cut(s) 145
BshFI GGCC 3 cut(s) 143, 302, 327
BshNI GGYRCC 1 cut(s) 78
BsiHKCI CYCGRG 1 cut(s) 257
BsiSI CCGG 1 cut(s) 271
BslI CCNNNNNNNGG 1 cut(s) 139
BsmAI GTCTC 1 cut(s) 279
BsmI GAATGC 1 cut(s) 10
BsnI GGCC 3 cut(s) 143, 302, 327
Bso31I GGTCTC 1 cut(s) 279
BsoBI CYCGRG 1 cut(s) 257
Bsp120I GGGCCC 1 cut(s) 141
Bsp1286I GDGCHC 1 cut(s) 145
BspACI CCGC 2 cut(s) 8, 199
BspANI GGCC 3 cut(s) 143, 302, 327
BspCNI CTCAG 1 cut(s) 220
BspHI TCATGA 1 cut(s) 88
BspLI GGNNCC 3 cut(s) 80, 131, 143
BspT107I GGYRCC 1 cut(s) 78
BspTNI GGTCTC 1 cut(s) 279
BsrI ACTGG 1 cut(s) 45
BssNI GRCGYC 1 cut(s) 170
Bst2UI CCWGG 1 cut(s) 134
Bst4CI ACNGT 2 cut(s) 42, 151
BstACI GRCGYC 1 cut(s) 170
BstAPI GCANNNNNTGC 1 cut(s) 20
BstC8I GCNNGC 3 cut(s) 247, 289, 293
BstDEI CTNAG 1 cut(s) 228
BstF5I GGATG 1 cut(s) 195
BstMAI GTCTC 1 cut(s) 279
BstMWI GCNNNNNNNGC 1 cut(s) 20
BstNI CCWGG 1 cut(s) 134
BstSCI CCNGG 1 cut(s) 132
BstSLI GKGCMC 1 cut(s) 145
BsuRI GGCC 3 cut(s) 143, 302, 327
BtsCI GGATG 1 cut(s) 195
BtsIMutI CAGTG 1 cut(s) 69
Cac8I GCNNGC 3 cut(s) 247, 289, 293
CciI TCATGA 1 cut(s) 88
Cfr13I GGNCC 3 cut(s) 42, 141, 142
Csp6I GTAC 1 cut(s) 79
CviAII CATG 2 cut(s) 55, 89
CviJI RGCY 6 cut(s) 143, 245, 268, 295, 302, 327
CviKI_1 RGCY 6 cut(s) 143, 245, 268, 295, 302, 327
CviQI GTAC 1 cut(s) 79
DdeI CTNAG 1 cut(s) 228
Eco147I AGGCCT 1 cut(s) 327
Eco24I GRGCYC 1 cut(s) 145
Eco31I GGTCTC 1 cut(s) 279
Eco47I GGWCC 1 cut(s) 42
Eco88I CYCGRG 1 cut(s) 257
EcoO109I RGGNCCY 1 cut(s) 142
EcoRII CCWGG 1 cut(s) 132
EcoT38I GRGCYC 1 cut(s) 145
FaeI CATG 2 cut(s) 58, 92
FaiI YATR 3 cut(s) 56, 90, 155
FatI CATG 2 cut(s) 54, 88
FauI CCCGC 1 cut(s) 192
FokI GGATG 1 cut(s) 202
FriOI GRGCYC 1 cut(s) 145
GsuI CTGGAG 1 cut(s) 155
HaeIII GGCC 3 cut(s) 143, 302, 327
HapII CCGG 1 cut(s) 271
Hin1I GRCGYC 1 cut(s) 170
Hin1II CATG 2 cut(s) 58, 92
HinfI GANTC 5 cut(s) 59, 67, 106, 237, 253
HpaII CCGG 1 cut(s) 271
HphI GGTGA 2 cut(s) 124, 130
Hpy188I TCNGA 2 cut(s) 229, 323
Hpy188III TCNNGA 4 cut(s) 89, 257, 278, 313
Hpy99I CGWCG 1 cut(s) 175
HpyAV CCTTC 1 cut(s) 109
HpyCH4III ACNGT 2 cut(s) 42, 151
HpyCH4IV ACGT 1 cut(s) 170
HpyCH4V TGCA 1 cut(s) 157
HpyF10VI GCNNNNNNNGC 1 cut(s) 20
HpyF3I CTNAG 1 cut(s) 228
HpySE526I ACGT 1 cut(s) 170
Hsp92I GRCGYC 1 cut(s) 170
Hsp92II CATG 2 cut(s) 58, 92
KpnI GGTACC 1 cut(s) 82
LmnI GCTCC 2 cut(s) 202, 273
LpnPI CCDG 6 cut(s) 35, 58, 119, 146, 158, 284
MaeII ACGT 1 cut(s) 170
MboII GAAGA 1 cut(s) 175
MfeI CAATTG 1 cut(s) 95
MhlI GDGCHC 1 cut(s) 145
MluCI AATT 1 cut(s) 95
MlyI GAGTC 2 cut(s) 68, 76
MnlI CCTC 7 cut(s) 95, 153, 180, 227, 317, 317, 320
MslI CAYNNNNRTG 1 cut(s) 87
MspI CCGG 1 cut(s) 271
MspR9I CCNGG 1 cut(s) 134
MunI CAATTG 1 cut(s) 95
Mva1269I GAATGC 1 cut(s) 10
MvaI CCWGG 1 cut(s) 134
MwoI GCNNNNNNNGC 1 cut(s) 20
NlaIII CATG 2 cut(s) 58, 92
NlaIV GGNNCC 3 cut(s) 80, 131, 143
PaeR7I CTCGAG 1 cut(s) 257
PagI TCATGA 1 cut(s) 88
PceI AGGCCT 1 cut(s) 327
PctI GAATGC 1 cut(s) 10
PfeI GAWTC 3 cut(s) 106, 237, 253
PleI GAGTC 2 cut(s) 67, 75
PpsI GAGTC 2 cut(s) 67, 75
Psp6I CCWGG 1 cut(s) 132
PspGI CCWGG 1 cut(s) 132
PspN4I GGNNCC 3 cut(s) 80, 131, 143
PspOMI GGGCCC 1 cut(s) 141
PspPI GGNCC 3 cut(s) 42, 141, 142
RsaI GTAC 1 cut(s) 80
RsaNI GTAC 1 cut(s) 79
RseI CAYNNNNRTG 1 cut(s) 87
Sau96I GGNCC 3 cut(s) 42, 141, 142
SchI GAGTC 2 cut(s) 68, 76
ScrFI CCNGG 1 cut(s) 134
SduI GDGCHC 1 cut(s) 145
SetI ASST 7 cut(s) 120, 135, 173, 219, 270, 285, 297
Sfr274I CTCGAG 1 cut(s) 257
SinI GGWCC 1 cut(s) 42
SlaI CTCGAG 1 cut(s) 257
SmiMI CAYNNNNRTG 1 cut(s) 87
SmlI CTYRAG 4 cut(s) 257, 276, 296, 311
SmoI CTYRAG 4 cut(s) 257, 276, 296, 311
Sse9I AATT 1 cut(s) 95
SseBI AGGCCT 1 cut(s) 327
SsiI CCGC 2 cut(s) 8, 199
StuI AGGCCT 1 cut(s) 327
StyD4I CCNGG 1 cut(s) 132
TaaI ACNGT 2 cut(s) 42, 151
TaiI ACGT 1 cut(s) 173
TaqI TCGA 2 cut(s) 173, 258
TasI AATT 1 cut(s) 95
TfiI GAWTC 3 cut(s) 106, 237, 253
TscAI CASTG 1 cut(s) 69
TspDTI ATGAA 2 cut(s) 17, 137
TspRI CASTG 1 cut(s) 69
VpaK11BI GGWCC 1 cut(s) 42
XcmI CCANNNNNNNNNTGG 1 cut(s) 52
XhoI CTCGAG 1 cut(s) 257
ZraI GACGTC 1 cut(s) 171
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.