pycom09g19830

Glycosyl hydrolases family 28

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0007246
Physical Location & Seq
Reverse (-)
20690899 .. 20693884
2986 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1314 bp
ATGATGAGGACGACGACATCTTCTTCTTCTTCTTCTTCCACTCTCACTGTCACTGTGGTGGACGTGCTTCTGCTACTTGCATTGCTTGGCGCTGCTGCTCCGTCGTCTGTCAAAGGCAGCCCACAGTGCAGCAATCAGGCCAACTCAGGGGAAATTCGACCTCACAGTGTCGCCATCACTGAATTTGGCGCTGTAGGAGATGGGGTCACTCTCAACACAAAAGCCTTTCAGAATGCCGTCTTCTATCTCAACTCTTTCACACGCAAGGGTGGGGCCAAGCTTTTTGTCCCTGCTGGCCGCTGGTTGACAGGAAGCTTCCGTCTCATCAGTCATCTAACCCTCTGGTTGGATAAGGACGCTGTCATTCTTGGATCAACGAAATCTGATGATTGGCCAATTGTTGAACCATTACCATCATACGGTCGAGGAAGGGAGTTGGCAGGAGGAAGACATCGAAGTCTCATCTATGGATGCGATTTGACAGACATTATCATAACAGGTGATAATGGTACCATTGATGGCCAAGGTAGCATCTGGTGGGATTGGTTCCATAGCAAAACCCTGAATTATACGAGGCCCCATTTGTTGGAGTTGATAAACTCAACTGGGGTTGTCATCTCAAACTTGACCTTCCTAAATTCACCATTTTGGACCATTCACCCTGTATATTGCAGCCATGTAACTATCCAGAATCTGACAATCCTTGCTCCTCTCAAGTCTCCAAACACAGACGGGATTGATCCAGATTCTTCAGATAATGTGTGCATTGAAGACTGTTATATTAGCACTGGTGATGATCTGATTTCCATCAAAAGTGGGTGGGATGAGTATGGAATTTCATATGGTCGACCCAGTAGAAACATCATCATTCACAGGATTACCGGTAAAACTGAAACAAGCGCTGGAATTGCAATTGGAAGTGAGATGTCAGGAGGCATAGCTTTAAGGTTCACCAGTCAGTATGGGGAACACCCAGATGAGTTTTACGACCCAAATGCCCTCCCTAATGTAGAGCGAATTACTTTTAAAGATATCATAGGAGAGAATATCAAAACTGCTGGTCTCCTAGAGGGTTTAGAAGGTGAAAATTTTCTGAACATTTGCCTATCTAATATTACCCTTAACGTGACCTCAAAATCTCCATGGAAATGCTCTTCTGTTCAAGGATATTCCAACCTAGTTTCTCCAGAAACTTGTGCGCCTCTCAAGGAGAATCTCTCCCCACGGCATTATTCAGATTGTTACTCTCTATCTGATGACATATGGATTTCTTCCAGTAATCAAAACAGAGGTGCTCGGTGGCTATCTTGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

438

Amino Acids

47.68

Weight (kDa)

5.48

Isoelectric Point (pI)

39.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 153 - 309 3.3e-27 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 456
Acc65I GGTACC 1 cut(s) 509
AccB1I GGYRCC 1 cut(s) 509
AccB7I CCANNNNNTGG 1 cut(s) 586
AccI GTMKAC 1 cut(s) 847
AciI CCGC 1 cut(s) 298
AclWI GGATC 2 cut(s) 379, 734
AcoI YGGCCR 3 cut(s) 295, 392, 520
AcsI RAATTY 5 cut(s) 153, 182, 637, 834, 1087
AcuI CTGAAG 1 cut(s) 735
AfaI GTAC 1 cut(s) 511
AfeI AGCGCT 1 cut(s) 901
AfiI CCNNNNNNNGG 4 cut(s) 147, 346, 419, 586
AgeI ACCGGT 1 cut(s) 881
AgsI TTSAA 3 cut(s) 404, 770, 1163
AjiI CACGTC 1 cut(s) 64
AleI CACNNNNGTG 1 cut(s) 56
AluBI AGCT 3 cut(s) 280, 315, 941
AluI AGCT 3 cut(s) 280, 315, 941
Alw21I GWGCWC 1 cut(s) 1297
Alw26I GTCTC 4 cut(s) 326, 464, 723, 1067
AlwI GGATC 2 cut(s) 379, 734
AlwNI CAGNNNCTG 1 cut(s) 694
Aor51HI AGCGCT 1 cut(s) 901
AoxI GGCC 6 cut(s) 138, 273, 295, 392, 520, 575
ApeKI GCWGC 5 cut(s) 92, 95, 117, 129, 672
ApoI RAATTY 5 cut(s) 153, 182, 637, 834, 1087
AsiGI ACCGGT 1 cut(s) 881
Asp700I GAANNNNTTC 1 cut(s) 1089
Asp718I GGTACC 1 cut(s) 509
AspLEI GCGC 4 cut(s) 92, 191, 902, 1201
AspS9I GGNCC 3 cut(s) 273, 576, 651
AsuHPI GGTGA 6 cut(s) 512, 633, 650, 803, 943, 1094
AvaII GGWCC 1 cut(s) 651
BalI TGGCCA 2 cut(s) 394, 522
BanI GGYRCC 1 cut(s) 509
BbsI GAAGAC 3 cut(s) 232, 454, 777
Bbv12I GWGCWC 1 cut(s) 1297
BbvI GCAGC 5 cut(s) 79, 82, 129, 141, 684
BccI CCATC 5 cut(s) 182, 194, 421, 512, 815
BceAI ACGGC 2 cut(s) 221, 1241
BcgI CGANNNNNNTGC 2 cut(s) 977, 1011
BcoDI GTCTC 4 cut(s) 326, 464, 723, 1067
BfaI CTAG 2 cut(s) 1067, 1178
BfmI CTRYAG 1 cut(s) 192
BfoI RGCGCY 3 cut(s) 93, 192, 903
BisI GCNGC 6 cut(s) 93, 96, 118, 130, 298, 673
BlsI GCNGC 6 cut(s) 94, 97, 119, 131, 299, 674
Bme18I GGWCC 1 cut(s) 651
BmgBI CACGTC 1 cut(s) 64
BmgT120I GGNCC 3 cut(s) 273, 576, 651
BmiI GGNNCC 4 cut(s) 274, 511, 548, 578
BmrI ACTGGG 2 cut(s) 615, 846
BmsI GCATC 2 cut(s) 461, 540
BmuI ACTGGG 2 cut(s) 615, 846
BpiI GAAGAC 3 cut(s) 232, 454, 777
BplI GAGNNNNNCTC 2 cut(s) 1202, 1234
BpmI CTGGAG 1 cut(s) 1170
BpuEI CTTGAG 2 cut(s) 698, 1190
BsaBI GATNNNNATC 1 cut(s) 806
BsaI GGTCTC 1 cut(s) 1067
BsaJI CCNNGG 3 cut(s) 523, 1142, 1223
BsaWI WCCGGW 1 cut(s) 881
BsaXI ACNNNNNCTCC 2 cut(s) 189, 219
Bsc4I CCNNNNNNNGG 4 cut(s) 147, 346, 419, 586
Bse118I RCCGGY 1 cut(s) 881
Bse1I ACTGG 5 cut(s) 610, 793, 852, 954, 1275
Bse3DI GCAATG 1 cut(s) 80
Bse8I GATNNNNATC 1 cut(s) 806
BseDI CCNNGG 3 cut(s) 523, 1142, 1223
BseGI GGATG 2 cut(s) 476, 829
BseJI GATNNNNATC 1 cut(s) 806
BseLI CCNNNNNNNGG 4 cut(s) 147, 346, 419, 586
BseMI GCAATG 1 cut(s) 80
BseMII CTCAG 1 cut(s) 159
BseNI ACTGG 5 cut(s) 610, 793, 852, 954, 1275
BseRI GAGGAG 1 cut(s) 699
BseXI GCAGC 5 cut(s) 79, 82, 129, 141, 684
BsgI GTGCAG 1 cut(s) 148
Bsh1285I CGRYCG 1 cut(s) 424
BshFI GGCC 6 cut(s) 140, 275, 297, 394, 522, 577
BshNI GGYRCC 1 cut(s) 509
BshTI ACCGGT 1 cut(s) 881
BsiEI CGRYCG 1 cut(s) 424
BsiHKAI GWGCWC 1 cut(s) 1297
BsiSI CCGG 1 cut(s) 882
BslFI GGGAC 1 cut(s) 272
BslI CCNNNNNNNGG 4 cut(s) 147, 346, 419, 586
BsmAI GTCTC 4 cut(s) 326, 464, 723, 1067
BsmBI CGTCTC 1 cut(s) 326
BsmFI GGGAC 1 cut(s) 272
BsmI GAATGC 1 cut(s) 238
BsnI GGCC 6 cut(s) 140, 275, 297, 394, 522, 577
Bso31I GGTCTC 1 cut(s) 1067
Bsp1286I GDGCHC 1 cut(s) 1297
Bsp143I GATC 3 cut(s) 371, 739, 796
Bsp19I CCATGG 1 cut(s) 1142
BspACI CCGC 1 cut(s) 298
BspANI GGCC 6 cut(s) 140, 275, 297, 394, 522, 577
BspCNI CTCAG 1 cut(s) 158
BspLI GGNNCC 4 cut(s) 274, 511, 548, 578
BspPI GGATC 2 cut(s) 379, 734
BspQI GCTCTTC 1 cut(s) 1159
BspT107I GGYRCC 1 cut(s) 509
BspTNI GGTCTC 1 cut(s) 1067
BsrDI GCAATG 1 cut(s) 80
BsrFI RCCGGY 1 cut(s) 881
BsrI ACTGG 5 cut(s) 610, 793, 852, 954, 1275
BssAI RCCGGY 1 cut(s) 881
BssECI CCNNGG 3 cut(s) 523, 1142, 1223
BssMI GATC 3 cut(s) 371, 739, 796
BssT1I CCWWGG 2 cut(s) 523, 1142
Bst4CI ACNGT 6 cut(s) 49, 55, 126, 167, 422, 776
Bst6I CTCTTC 1 cut(s) 1159
BstC8I GCNNGC 1 cut(s) 295
BstDEI CTNAG 1 cut(s) 145
BstDSI CCRYGG 2 cut(s) 1142, 1223
BstF5I GGATG 2 cut(s) 476, 829
BstH2I RGCGCY 3 cut(s) 93, 192, 903
BstHHI GCGC 4 cut(s) 92, 191, 902, 1201
BstKTI GATC 3 cut(s) 374, 742, 799
BstMAI GTCTC 4 cut(s) 326, 464, 723, 1067
BstMBI GATC 3 cut(s) 371, 739, 796
BstMCI CGRYCG 1 cut(s) 424
BstMWI GCNNNNNNNGC 3 cut(s) 126, 528, 908
BstSFI CTRYAG 1 cut(s) 192
BstV1I GCAGC 5 cut(s) 79, 82, 129, 141, 684
BstV2I GAAGAC 3 cut(s) 232, 454, 777
BsuRI GGCC 6 cut(s) 140, 275, 297, 394, 522, 577
BtgI CCRYGG 2 cut(s) 1142, 1223
BtrI CACGTC 1 cut(s) 64
BtsCI GGATG 2 cut(s) 476, 829
BtsIMutI CAGTG 6 cut(s) 45, 51, 131, 172, 177, 786
Cac8I GCNNGC 1 cut(s) 295
CaiI CAGNNNCTG 1 cut(s) 694
CfoI GCGC 4 cut(s) 92, 191, 902, 1201
Cfr10I RCCGGY 1 cut(s) 881
Cfr13I GGNCC 3 cut(s) 273, 576, 651
CseI GACGC 1 cut(s) 365
Csp6I GTAC 1 cut(s) 510
CspAI ACCGGT 1 cut(s) 881
CviAII CATG 2 cut(s) 677, 1143
CviQI GTAC 1 cut(s) 510
DdeI CTNAG 1 cut(s) 145
DpnI GATC 3 cut(s) 373, 741, 798
DpnII GATC 3 cut(s) 371, 739, 796
DraI TTTAAA 1 cut(s) 1027
DrdI GACNNNNNNGTC 1 cut(s) 456
DseDI GACNNNNNNGTC 1 cut(s) 456
EaeI YGGCCR 3 cut(s) 295, 392, 520
Eam1104I CTCTTC 1 cut(s) 1159
EarI CTCTTC 1 cut(s) 1159
Eco130I CCWWGG 2 cut(s) 523, 1142
Eco31I GGTCTC 1 cut(s) 1067
Eco32I GATATC 1 cut(s) 1033
Eco47I GGWCC 1 cut(s) 651
Eco47III AGCGCT 1 cut(s) 901
Eco57I CTGAAG 1 cut(s) 735
EcoO109I RGGNCCY 1 cut(s) 576
EcoRV GATATC 1 cut(s) 1033
EcoT14I CCWWGG 2 cut(s) 523, 1142
ErhI CCWWGG 2 cut(s) 523, 1142
Esp3I CGTCTC 1 cut(s) 326
FaeI CATG 2 cut(s) 680, 1146
FaqI GGGAC 1 cut(s) 272
FatI CATG 2 cut(s) 676, 1142
FauNDI CATATG 2 cut(s) 841, 1262
FblI GTMKAC 1 cut(s) 847
Fnu4HI GCNGC 6 cut(s) 93, 96, 118, 130, 298, 673
FokI GGATG 2 cut(s) 483, 836
Fsp4HI GCNGC 6 cut(s) 93, 96, 118, 130, 298, 673
FspBI CTAG 2 cut(s) 1067, 1178
GlaI GCGC 4 cut(s) 91, 190, 901, 1200
GluI GCNGC 6 cut(s) 93, 96, 118, 130, 298, 673
GsuI CTGGAG 1 cut(s) 1170
HaeII RGCGCY 3 cut(s) 93, 192, 903
HaeIII GGCC 6 cut(s) 140, 275, 297, 394, 522, 577
HapII CCGG 1 cut(s) 882
HgaI GACGC 1 cut(s) 365
HhaI GCGC 4 cut(s) 92, 191, 902, 1201
Hin1II CATG 2 cut(s) 680, 1146
Hin6I GCGC 4 cut(s) 90, 189, 900, 1199
HinP1I GCGC 4 cut(s) 90, 189, 900, 1199
HincII GTYRAC 2 cut(s) 306, 848
HindII GTYRAC 2 cut(s) 306, 848
HindIII AAGCTT 2 cut(s) 278, 313
HinfI GANTC 3 cut(s) 691, 746, 1213
HpaII CCGG 1 cut(s) 882
HphI GGTGA 6 cut(s) 512, 633, 650, 803, 943, 1094
Hpy166II GTNNAC 4 cut(s) 61, 306, 848, 951
Hpy188I TCNGA 8 cut(s) 231, 385, 696, 754, 801, 1095, 1237, 1255
Hpy188III TCNNGA 4 cut(s) 688, 743, 930, 1187
Hpy8I GTNNAC 4 cut(s) 61, 306, 848, 951
Hpy99I CGWCG 2 cut(s) 16, 106
HpyAV CCTTC 3 cut(s) 423, 640, 1073
HpyCH4III ACNGT 6 cut(s) 49, 55, 126, 167, 422, 776
HpyCH4IV ACGT 2 cut(s) 63, 1125
HpyCH4V TGCA 5 cut(s) 80, 129, 672, 765, 911
HpyF10VI GCNNNNNNNGC 3 cut(s) 126, 528, 908
HpyF3I CTNAG 1 cut(s) 145
HpySE526I ACGT 2 cut(s) 63, 1125
Hsp92II CATG 2 cut(s) 680, 1146
HspAI GCGC 4 cut(s) 90, 189, 900, 1199
KpnI GGTACC 1 cut(s) 513
Kzo9I GATC 3 cut(s) 371, 739, 796
LguI GCTCTTC 1 cut(s) 1159
LmnI GCTCC 2 cut(s) 103, 712
Lsp1109I GCAGC 5 cut(s) 79, 82, 129, 141, 684
LweI GCATC 2 cut(s) 461, 540
MaeI CTAG 2 cut(s) 1067, 1178
MaeII ACGT 2 cut(s) 63, 1125
MaeIII GTNAC 5 cut(s) 49, 205, 679, 1126, 1241
MalI GATC 3 cut(s) 373, 741, 798
MboI GATC 3 cut(s) 371, 739, 796
MfeI CAATTG 2 cut(s) 396, 912
MhlI GDGCHC 1 cut(s) 1297
MlsI TGGCCA 2 cut(s) 394, 522
MluNI TGGCCA 2 cut(s) 394, 522
MmeI TCCRAC 3 cut(s) 327, 567, 1197
Mox20I TGGCCA 2 cut(s) 394, 522
MroXI GAANNNNTTC 1 cut(s) 1089
MscI TGGCCA 2 cut(s) 394, 522
MseI TTAA 3 cut(s) 944, 1026, 1122
MslI CAYNNNNRTG 3 cut(s) 56, 975, 1147
Msp20I TGGCCA 2 cut(s) 394, 522
MspA1I CMGCKG 1 cut(s) 300
MspI CCGG 1 cut(s) 882
MunI CAATTG 2 cut(s) 396, 912
Mva1269I GAATGC 1 cut(s) 238
MwoI GCNNNNNNNGC 3 cut(s) 126, 528, 908
NcoI CCATGG 1 cut(s) 1142
NdeI CATATG 2 cut(s) 841, 1262
NdeII GATC 3 cut(s) 371, 739, 796
NlaIII CATG 2 cut(s) 680, 1146
NlaIV GGNNCC 4 cut(s) 274, 511, 548, 578
NmuCI GTSAC 3 cut(s) 49, 205, 1126
OliI CACNNNNGTG 1 cut(s) 56
PciSI GCTCTTC 1 cut(s) 1159
PctI GAATGC 1 cut(s) 238
PdmI GAANNNNTTC 1 cut(s) 1089
PfeI GAWTC 3 cut(s) 691, 746, 1213
PflFI GACNNNGTC 1 cut(s) 359
PflMI CCANNNNNTGG 1 cut(s) 586
PinAI ACCGGT 1 cut(s) 881
PkrI GCNGC 6 cut(s) 94, 97, 119, 131, 299, 674
PspN4I GGNNCC 4 cut(s) 274, 511, 548, 578
PspPI GGNCC 3 cut(s) 273, 576, 651
PstNI CAGNNNCTG 1 cut(s) 694
PsyI GACNNNGTC 1 cut(s) 359
RsaI GTAC 1 cut(s) 511
RsaNI GTAC 1 cut(s) 510
RseI CAYNNNNRTG 3 cut(s) 56, 975, 1147
SalI GTCGAC 1 cut(s) 846
SapI GCTCTTC 1 cut(s) 1159
SaqAI TTAA 3 cut(s) 944, 1026, 1122
SatI GCNGC 6 cut(s) 93, 96, 118, 130, 298, 673
Sau3AI GATC 3 cut(s) 371, 739, 796
Sau96I GGNCC 3 cut(s) 273, 576, 651
SduI GDGCHC 1 cut(s) 1297
SfaNI GCATC 2 cut(s) 461, 540
SfcI CTRYAG 1 cut(s) 192
SinI GGWCC 1 cut(s) 651
SmiMI CAYNNNNRTG 3 cut(s) 56, 975, 1147
SmlI CTYRAG 2 cut(s) 713, 1205
SmoI CTYRAG 2 cut(s) 713, 1205
SsiI CCGC 1 cut(s) 298
SspI AATATT 1 cut(s) 1114
SspMI CTAG 2 cut(s) 1067, 1178
StyI CCWWGG 2 cut(s) 523, 1142
TaaI ACNGT 6 cut(s) 49, 55, 126, 167, 422, 776
TaiI ACGT 2 cut(s) 66, 1128
TaqI TCGA 4 cut(s) 157, 424, 454, 847
TauI GCSGC 1 cut(s) 300
TfiI GAWTC 3 cut(s) 691, 746, 1213
Tru1I TTAA 3 cut(s) 944, 1026, 1122
Tru9I TTAA 3 cut(s) 944, 1026, 1122
TscAI CASTG 6 cut(s) 52, 58, 131, 172, 184, 793
TseFI GTSAC 3 cut(s) 49, 205, 1126
TseI GCWGC 5 cut(s) 92, 95, 117, 129, 672
Tsp45I GTSAC 3 cut(s) 49, 205, 1126
TspDTI ATGAA 1 cut(s) 828
TspGWI ACGGA 2 cut(s) 90, 308
TspRI CASTG 6 cut(s) 52, 58, 131, 172, 184, 793
Tth111I GACNNNGTC 1 cut(s) 359
Van91I CCANNNNNTGG 1 cut(s) 586
VpaK11BI GGWCC 1 cut(s) 651
XapI RAATTY 5 cut(s) 153, 182, 637, 834, 1087
XmiI GTMKAC 1 cut(s) 847
XmnI GAANNNNTTC 1 cut(s) 1089
XspI CTAG 2 cut(s) 1067, 1178
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.