Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr10

Orthogroup ID

OG0006699

Physical Location & Seq

Genomic Coordinates Reverse (-)

297948 .. 300103

Gene Structure 2156 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1524 bp

ATGACATTGAAGAAATGTCAAATAGTAGCTCGGTTGTTCTTACAACCGTGCTCAAGCTCATTGACAAGTATCGATCTGTATGGGCAAGTGGCCTATCCTAAGCATCACAAGCAATCAGATGTACCTGACATATATCGTCTAGCTGCAAAAACAAAGGGAGTTTTCGTCAACCCAGCTCTCGTGGAACCTTTCGGGCTCACAATTATAGAGGCAGCTGCTTACGGTTTACCAGTGGTCGCCACCAAAAATGGCGGACCTGTGGATATTCTGAAGGCACTAAACAACGGCCTCCTAGTTGACCCACATGACCAGAAAGCCATAGAAGAGGCACTGCTAAAGCTTGTAGGAGACAAAAACCTGTGGCTCGAATGCAGAAAGAACGGCCTAAAGAACATCCACCGCTTTTCGTGGCCGGAACACTGCCGGAACTACCTCTCCCATGTTGAACATTCCCGAAACCGTCACCCCACTACCCGCCTTCAGATCACGCCAATTCCTGAAGAACCATTGAGTGACTCTCTAAAAGATGTGGAAGACCTTTCTTTGAGATTCTCTGTAGAAGGAGACTTCAAGCATAATGGAGAGCTCGATGCAGCAACTAGACAGAGGGAACTGATTGAAGCCATAACTAGAATGTCTTCGTCCAGTAGCGATATCGGTGCTACTTATGGTCCTGGCAGAAGGCAGAGGCTATTTGTAATAGCCGTGGATTGTTATGACCAGAATGGAGACGGCACCCAAGTATTTCAAGAAGTTCTAGTGAATGTAAAGAAAGTAGCAAGCTTAGGCTATGGCCAGGGTCGAGTAGGCATTGTGTTGTTAACTGGTTCCAGCTTACAAGATATCGTCAAAGCGTTTAAAGGCTGCCAAATAAACATTGAAGATTTTGACGCGTTGGTCTGCAAAAGTGGGGGTGAAATGTACTACCCGTGGAGGGATTTGGCAGCTGACGCAGATTATGAAACCCATATTGAGTACAGATGGCCTGGCGAGAATGTGAGGTCCATGGTTCCAAGGCTTGCCAGGCTAGAAGTTGGGGATGATGACGATATCGTGGAGTATGCTGGATCAAGTAGCTCTAGGTGCTACTCTTATAGTGTGAGGCCAGGAGCCAAGACTCGACGGGTTGATGACCTACGCCAACGGCTGCGGATGAGAGGCTTCCGATGCAACCTTGTCTACACTCGTGCAGCATCAAGGTTAAATGTTGTACCCCTAGTTGCATCAAGAGTACAAGCACTAAGATATCTTTCCGTCAGGTGGGCCATTGATCTGTCAAAAGTGGTTGTATTTGTTGGGGAAAAAGGAGATACAGACATTGAAGACCTGCTGGCCGGCCTCCACAAGACCCTTGTTCTAAGAGGTTCTGTTGAGTATGGCAGCGAGAAGCTTATTCACGGTGAAGATAGTTTCAAAAGAGAAGATGTAGTCCCACAAGATAGCCCTAACATTGCCCTTGTGGAGAGCTATCAAGCCCATGACATCTCAGCGGCACTTGAAGCACTTGGGATCAAGGGATTTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005623 GO:0005886 GO:0008194 GO:0016020 GO:0016740 GO:0016757 GO:0016758 GO:0035251 GO:0044464 GO:0046524 GO:0046527 GO:0071944

KEGG Pathways

Metabolic & Signaling

ko00500 ko01100 map00500 map01100

Pfam Domains

Protein Families

Glyco_trans_4_4 Glycos_transf_1 S6PP Sucrose_synth

Protein Analysis

508

Amino Acids

56.5

Weight (kDa)

6.69

Isoelectric Point (pI)

42.42

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glycos_transf_1	PF00534	29 - 129	4.8e-19	Glycosyl transferases group 1
Glyco_trans_1_4	PF13692	39 - 117	2.3e-14	Glycosyl transferases group 1
S6PP	PF05116	266 - 439	9.1e-19	Sucrose-6F-phosphate phosphohydrolase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0006699)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G10120 AT4G10120 AT4G10120 AT4G10120 AT4G10120
fragaria_vesca	FvH4_2g00340 FvH4_2g00340 FvH4_2g00340
malus_domestica	MD05G1006400.v1.1 MD10G1002300.v1.1 MD10G1002500.v1.1
prunus_persica	Prupe.8G003700_v2.0.a1
pyrus_communis	pycom05g00160 pycom10g00210
rosa_chinensis	RchiOBHm_Chr6g0249631
rosa_laevigata	RLG00000015556
rosa_multiflora	Rmu_sc0006936.1_g000016 Rmu_sc0029595.1_g000001
rosa_roxburghii	Rroxscaffold_7G00217470
rosa_rugosa	Rorug05G0494200 Rorug05G0494200
rosa_samantha	Rh6AG004100 Rh6BG006100 Rh6CG003100 Rh6DG002900
rosa_wichuraiana	Rw6G000450

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	896
Acc36I	ACCTGC	1 cut(s)	1335
AccB1I	GGYRCC	1 cut(s)	734
AccI	GTMKAC	1 cut(s)	1179
AccII	CGCG	1 cut(s)	893
AciI	CCGC	5 cut(s)	252, 400, 475, 1150, 1490
AclWI	GGATC	2 cut(s)	1075, 1517
AcoI	YGGCCR	3 cut(s)	410, 793, 1332
AcuI	CTGAAG	3 cut(s)	290, 464, 519
AfaI	GTAC	5 cut(s)	123, 923, 977, 1212, 1233
AfiI	CCNNNNNNNGG	2 cut(s)	934, 1260
AflIII	ACRYGT	1 cut(s)	891
AgsI	TTSAA	9 cut(s)	10, 446, 571, 620, 749, 881, 1322, 1414, 1499
AjnI	CCWGG	5 cut(s)	673, 795, 985, 1022, 1105
AloI	GAACNNNNNNTCC	2 cut(s)	419, 451
Alw21I	GWGCWC	2 cut(s)	53, 588
Alw26I	GTCTC	3 cut(s)	342, 558, 723
AlwI	GGATC	2 cut(s)	1075, 1517
ApeKI	GCWGC	9 cut(s)	143, 212, 215, 593, 864, 944, 1147, 1190, 1380
Asp700I	GAANNNNTTC	1 cut(s)	637
AspS9I	GGNCC	4 cut(s)	254, 671, 1002, 1263
AsuHPI	GGTGA	3 cut(s)	455, 926, 1412
AvaII	GGWCC	3 cut(s)	254, 671, 1002
BalI	TGGCCA	1 cut(s)	795
BanI	GGYRCC	1 cut(s)	734
BanII	GRGCYC	2 cut(s)	198, 588
BauI	CACGAG	2 cut(s)	179, 1185
BbsI	GAAGAC	3 cut(s)	540, 630, 1329
Bbv12I	GWGCWC	2 cut(s)	53, 588
BbvI	GCAGC	9 cut(s)	130, 202, 224, 605, 851, 956, 1134, 1202, 1392
BccI	CCATC	1 cut(s)	975
BceAI	ACGGC	5 cut(s)	301, 397, 689, 748, 1160
BcgI	CGANNNNNNTGC	2 cut(s)	641, 675
BciT130I	CCWGG	5 cut(s)	675, 797, 987, 1024, 1107
BcoDI	GTCTC	3 cut(s)	342, 558, 723
BfaI	CTAG	8 cut(s)	140, 293, 600, 630, 758, 1028, 1080, 1217
BfmI	CTRYAG	1 cut(s)	555
BfuAI	ACCTGC	1 cut(s)	1335
Bme1390I	CCNGG	5 cut(s)	675, 797, 987, 1024, 1107
Bme18I	GGWCC	3 cut(s)	254, 671, 1002
BmgT120I	GGNCC	4 cut(s)	254, 671, 1002, 1263
BmiI	GGNNCC	5 cut(s)	186, 736, 829, 1011, 1111
BmrFI	CCNGG	5 cut(s)	675, 797, 987, 1024, 1107
BmsI	GCATC	5 cut(s)	112, 580, 1157, 1202, 1232
BpiI	GAAGAC	3 cut(s)	540, 630, 1329
BplI	GAGNNNNNCTC	2 cut(s)	502, 534
Bpu10I	CCTNAGC	2 cut(s)	99, 784
BpuEI	CTTGAG	1 cut(s)	37
Bsa29I	ATCGAT	1 cut(s)	72
BsaJI	CCNNGG	5 cut(s)	705, 796, 929, 1005, 1013
BsaXI	ACNNNNNCTCC	4 cut(s)	150, 180, 419, 449
Bsc4I	CCNNNNNNNGG	2 cut(s)	934, 1260
Bse118I	RCCGGY	1 cut(s)	1334
Bse1I	ACTGG	3 cut(s)	230, 645, 829
Bse3DI	GCAATG	1 cut(s)	1449
BseBI	CCWGG	5 cut(s)	675, 797, 987, 1024, 1107
BseCI	ATCGAT	1 cut(s)	72
BseDI	CCNNGG	5 cut(s)	705, 796, 929, 1005, 1013
BseGI	GGATG	3 cut(s)	393, 1045, 1158
BseLI	CCNNNNNNNGG	2 cut(s)	934, 1260
BseMI	GCAATG	1 cut(s)	1449
BseMII	CTCAG	1 cut(s)	1500
BseNI	ACTGG	3 cut(s)	230, 645, 829
BseXI	GCAGC	9 cut(s)	130, 202, 224, 605, 851, 956, 1134, 1202, 1392
BseYI	CCCAGC	1 cut(s)	172
BsgI	GTGCAG	1 cut(s)	1209
Bsh1236I	CGCG	1 cut(s)	893
BshNI	GGYRCC	1 cut(s)	734
BshVI	ATCGAT	1 cut(s)	72
BsiHKAI	GWGCWC	2 cut(s)	53, 588
BsiSI	CCGG	3 cut(s)	413, 424, 1335
BslFI	GGGAC	1 cut(s)	1415
BslI	CCNNNNNNNGG	2 cut(s)	934, 1260
BsmAI	GTCTC	3 cut(s)	342, 558, 723
BsmBI	CGTCTC	1 cut(s)	723
BsmFI	GGGAC	1 cut(s)	1415
BsmI	GAATGC	1 cut(s)	374
Bsp1286I	GDGCHC	3 cut(s)	53, 198, 588
Bsp143I	GATC	5 cut(s)	73, 483, 1067, 1270, 1509
Bsp19I	CCATGG	1 cut(s)	1005
BspACI	CCGC	5 cut(s)	252, 400, 475, 1150, 1490
BspCNI	CTCAG	1 cut(s)	1499
BspDI	ATCGAT	1 cut(s)	72
BspFNI	CGCG	1 cut(s)	893
BspLI	GGNNCC	5 cut(s)	186, 736, 829, 1011, 1111
BspMI	ACCTGC	1 cut(s)	1335
BspPI	GGATC	2 cut(s)	1075, 1517
BspT107I	GGYRCC	1 cut(s)	734
BsrDI	GCAATG	1 cut(s)	1449
BsrFI	RCCGGY	1 cut(s)	1334
BsrI	ACTGG	3 cut(s)	230, 645, 829
BssAI	RCCGGY	1 cut(s)	1334
BssECI	CCNNGG	5 cut(s)	705, 796, 929, 1005, 1013
BssMI	GATC	5 cut(s)	73, 483, 1067, 1270, 1509
BssSI	CACGAG	2 cut(s)	179, 1185
BssT1I	CCWWGG	2 cut(s)	1005, 1013
Bst2BI	CACGAG	2 cut(s)	179, 1185
Bst2UI	CCWGG	5 cut(s)	675, 797, 987, 1024, 1107
Bst4CI	ACNGT	4 cut(s)	48, 224, 461, 1400
Bst6I	CTCTTC	1 cut(s)	318
BstC8I	GCNNGC	4 cut(s)	781, 1020, 1332, 1336
BstDEI	CTNAG	5 cut(s)	99, 784, 1241, 1358, 1486
BstDSI	CCRYGG	3 cut(s)	705, 929, 1005
BstF5I	GGATG	3 cut(s)	393, 1045, 1158
BstFNI	CGCG	1 cut(s)	893
BstKTI	GATC	5 cut(s)	76, 486, 1070, 1273, 1512
BstMAI	GTCTC	3 cut(s)	342, 558, 723
BstMBI	GATC	5 cut(s)	73, 483, 1067, 1270, 1509
BstMWI	GCNNNNNNNGC	6 cut(s)	109, 950, 1024, 1083, 1167, 1499
BstNI	CCWGG	5 cut(s)	675, 797, 987, 1024, 1107
BstSCI	CCNGG	5 cut(s)	673, 795, 985, 1022, 1105
BstSFI	CTRYAG	1 cut(s)	555
BstUI	CGCG	1 cut(s)	893
BstV1I	GCAGC	9 cut(s)	130, 202, 224, 605, 851, 956, 1134, 1202, 1392
BstV2I	GAAGAC	3 cut(s)	540, 630, 1329
Bsu15I	ATCGAT	1 cut(s)	72
BsuTUI	ATCGAT	1 cut(s)	72
BtgI	CCRYGG	3 cut(s)	705, 929, 1005
BtsCI	GGATG	3 cut(s)	393, 1045, 1158
BtsI	GCAGTG	2 cut(s)	329, 418
BtsIMutI	CAGTG	3 cut(s)	237, 329, 418
BveI	ACCTGC	1 cut(s)	1335
Cac8I	GCNNGC	4 cut(s)	781, 1020, 1332, 1336
Cfr10I	RCCGGY	1 cut(s)	1334
Cfr13I	GGNCC	4 cut(s)	254, 671, 1002, 1263
ClaI	ATCGAT	1 cut(s)	72
CseI	GACGC	2 cut(s)	899, 959
Csp6I	GTAC	5 cut(s)	122, 922, 976, 1211, 1232
CviAII	CATG	4 cut(s)	305, 440, 1006, 1478
CviQI	GTAC	5 cut(s)	122, 922, 976, 1211, 1232
DdeI	CTNAG	5 cut(s)	99, 784, 1241, 1358, 1486
DpnI	GATC	5 cut(s)	75, 485, 1069, 1272, 1511
DpnII	GATC	5 cut(s)	73, 483, 1067, 1270, 1509
DraI	TTTAAA	1 cut(s)	859
DrdI	GACNNNNNNGTC	1 cut(s)	896
DseDI	GACNNNNNNGTC	1 cut(s)	896
EaeI	YGGCCR	3 cut(s)	410, 793, 1332
Eam1104I	CTCTTC	1 cut(s)	318
EarI	CTCTTC	1 cut(s)	318
EciI	GGCGGA	1 cut(s)	267
Ecl136II	GAGCTC	1 cut(s)	586
Eco130I	CCWWGG	2 cut(s)	1005, 1013
Eco24I	GRGCYC	2 cut(s)	198, 588
Eco32I	GATATC	4 cut(s)	655, 844, 1051, 1247
Eco47I	GGWCC	3 cut(s)	254, 671, 1002
Eco53kI	GAGCTC	1 cut(s)	586
Eco57I	CTGAAG	3 cut(s)	290, 464, 519
EcoICRI	GAGCTC	1 cut(s)	586
EcoRII	CCWGG	5 cut(s)	673, 795, 985, 1022, 1105
EcoRV	GATATC	4 cut(s)	655, 844, 1051, 1247
EcoT14I	CCWWGG	2 cut(s)	1005, 1013
EcoT38I	GRGCYC	2 cut(s)	198, 588
ErhI	CCWWGG	2 cut(s)	1005, 1013
Esp3I	CGTCTC	1 cut(s)	723
FaeI	CATG	4 cut(s)	308, 443, 1009, 1481
FaqI	GGGAC	1 cut(s)	1415
FatI	CATG	4 cut(s)	304, 439, 1005, 1477
FauI	CCCGC	1 cut(s)	482
FblI	GTMKAC	1 cut(s)	1179
FokI	GGATG	3 cut(s)	380, 1052, 1165
FriOI	GRGCYC	2 cut(s)	198, 588
FseI	GGCCGGCC	1 cut(s)	1338
FspBI	CTAG	8 cut(s)	140, 293, 600, 630, 758, 1028, 1080, 1217
GsaI	CCCAGC	1 cut(s)	176
HapII	CCGG	3 cut(s)	413, 424, 1335
HgaI	GACGC	2 cut(s)	899, 959
Hin1II	CATG	4 cut(s)	308, 443, 1009, 1481
HincII	GTYRAC	3 cut(s)	169, 298, 822
HindII	GTYRAC	3 cut(s)	169, 298, 822
HindIII	AAGCTT	3 cut(s)	338, 781, 1388
HinfI	GANTC	3 cut(s)	515, 549, 1117
HpaI	GTTAAC	1 cut(s)	822
HpaII	CCGG	3 cut(s)	413, 424, 1335
HphI	GGTGA	3 cut(s)	455, 926, 1412
Hpy166II	GTNNAC	5 cut(s)	169, 227, 298, 822, 1180
Hpy188I	TCNGA	4 cut(s)	118, 270, 483, 1166
Hpy188III	TCNNGA	4 cut(s)	453, 497, 749, 1227
Hpy8I	GTNNAC	5 cut(s)	169, 227, 298, 822, 1180
Hpy99I	CGWCG	1 cut(s)	1125
HpyAV	CCTTC	4 cut(s)	265, 488, 554, 675
HpyCH4III	ACNGT	4 cut(s)	48, 224, 461, 1400
HpyCH4V	TGCA	7 cut(s)	146, 372, 593, 903, 1170, 1190, 1223
HpyF10VI	GCNNNNNNNGC	6 cut(s)	109, 950, 1024, 1083, 1167, 1499
HpyF3I	CTNAG	5 cut(s)	99, 784, 1241, 1358, 1486
Hsp92II	CATG	4 cut(s)	308, 443, 1009, 1481
KroI	GCCGGC	1 cut(s)	1334
KroNI	GCCGGC	1 cut(s)	1336
KspAI	GTTAAC	1 cut(s)	822
Kzo9I	GATC	5 cut(s)	73, 483, 1067, 1270, 1509
LmnI	GCTCC	1 cut(s)	1109
Lsp1109I	GCAGC	9 cut(s)	130, 202, 224, 605, 851, 956, 1134, 1202, 1392
LweI	GCATC	5 cut(s)	112, 580, 1157, 1202, 1232
MaeI	CTAG	8 cut(s)	140, 293, 600, 630, 758, 1028, 1080, 1217
MaeIII	GTNAC	2 cut(s)	461, 512
MalI	GATC	5 cut(s)	75, 485, 1069, 1272, 1511
MboI	GATC	5 cut(s)	73, 483, 1067, 1270, 1509
MboII	GAAGA	9 cut(s)	22, 335, 512, 545, 630, 893, 1334, 1415, 1433
MhlI	GDGCHC	3 cut(s)	53, 198, 588
MlsI	TGGCCA	1 cut(s)	795
MluCI	AATT	2 cut(s)	201, 492
MluI	ACGCGT	1 cut(s)	891
MluNI	TGGCCA	1 cut(s)	795
MlyI	GAGTC	2 cut(s)	509, 1111
Mox20I	TGGCCA	1 cut(s)	795
MroNI	GCCGGC	1 cut(s)	1334
MroXI	GAANNNNTTC	1 cut(s)	637
MscI	TGGCCA	1 cut(s)	795
MseI	TTAA	3 cut(s)	821, 858, 1202
Msp20I	TGGCCA	1 cut(s)	795
MspA1I	CMGCKG	3 cut(s)	215, 947, 1490
MspI	CCGG	3 cut(s)	413, 424, 1335
MspR9I	CCNGG	5 cut(s)	675, 797, 987, 1024, 1107
Mva1269I	GAATGC	1 cut(s)	374
MvaI	CCWGG	5 cut(s)	675, 797, 987, 1024, 1107
MvnI	CGCG	1 cut(s)	893
MwoI	GCNNNNNNNGC	6 cut(s)	109, 950, 1024, 1083, 1167, 1499
NaeI	GCCGGC	1 cut(s)	1336
NcoI	CCATGG	1 cut(s)	1005
NdeII	GATC	5 cut(s)	73, 483, 1067, 1270, 1509
NgoMIV	GCCGGC	1 cut(s)	1334
NlaIII	CATG	4 cut(s)	308, 443, 1009, 1481
NlaIV	GGNNCC	5 cut(s)	186, 736, 829, 1011, 1111
NmuCI	GTSAC	2 cut(s)	461, 512
PctI	GAATGC	1 cut(s)	374
PdiI	GCCGGC	1 cut(s)	1336
PdmI	GAANNNNTTC	1 cut(s)	637
PfeI	GAWTC	1 cut(s)	549
PleI	GAGTC	2 cut(s)	509, 1111
PpsI	GAGTC	2 cut(s)	509, 1111
Psp124BI	GAGCTC	1 cut(s)	588
Psp6I	CCWGG	5 cut(s)	673, 795, 985, 1022, 1105
PspFI	CCCAGC	1 cut(s)	172
PspGI	CCWGG	5 cut(s)	673, 795, 985, 1022, 1105
PspN4I	GGNNCC	5 cut(s)	186, 736, 829, 1011, 1111
PspPI	GGNCC	4 cut(s)	254, 671, 1002, 1263
PvuII	CAGCTG	2 cut(s)	215, 947
RigI	GGCCGGCC	1 cut(s)	1338
RsaI	GTAC	5 cut(s)	123, 923, 977, 1212, 1233
RsaNI	GTAC	5 cut(s)	122, 922, 976, 1211, 1232
SacI	GAGCTC	1 cut(s)	588
SaqAI	TTAA	3 cut(s)	821, 858, 1202
Sau3AI	GATC	5 cut(s)	73, 483, 1067, 1270, 1509
Sau96I	GGNCC	4 cut(s)	254, 671, 1002, 1263
SchI	GAGTC	2 cut(s)	509, 1111
ScrFI	CCNGG	5 cut(s)	675, 797, 987, 1024, 1107
SduI	GDGCHC	3 cut(s)	53, 198, 588
SfaNI	GCATC	5 cut(s)	112, 580, 1157, 1202, 1232
SfcI	CTRYAG	1 cut(s)	555
SinI	GGWCC	3 cut(s)	254, 671, 1002
SmlI	CTYRAG	1 cut(s)	52
SmoI	CTYRAG	1 cut(s)	52
Sse9I	AATT	2 cut(s)	201, 492
SsiI	CCGC	5 cut(s)	252, 400, 475, 1150, 1490
SspMI	CTAG	8 cut(s)	140, 293, 600, 630, 758, 1028, 1080, 1217
SstI	GAGCTC	1 cut(s)	588
StyD4I	CCNGG	5 cut(s)	673, 795, 985, 1022, 1105
StyI	CCWWGG	2 cut(s)	1005, 1013
TaaI	ACNGT	4 cut(s)	48, 224, 461, 1400
TaqI	TCGA	5 cut(s)	72, 366, 588, 802, 1120
TasI	AATT	2 cut(s)	201, 492
TatI	WGTACW	3 cut(s)	921, 975, 1231
TauI	GCSGC	1 cut(s)	1493
TfiI	GAWTC	1 cut(s)	549
Tru1I	TTAA	3 cut(s)	821, 858, 1202
Tru9I	TTAA	3 cut(s)	821, 858, 1202
TscAI	CASTG	3 cut(s)	237, 336, 425
TseFI	GTSAC	2 cut(s)	461, 512
TseI	GCWGC	9 cut(s)	143, 212, 215, 593, 864, 944, 1147, 1190, 1380
Tsp45I	GTSAC	2 cut(s)	461, 512
TspDTI	ATGAA	1 cut(s)	975
TspGWI	ACGGA	1 cut(s)	1243
TspRI	CASTG	3 cut(s)	237, 336, 425
VpaK11BI	GGWCC	3 cut(s)	254, 671, 1002
XmiI	GTMKAC	1 cut(s)	1179
XmnI	GAANNNNTTC	1 cut(s)	637
XspI	CTAG	8 cut(s)	140, 293, 600, 630, 758, 1028, 1080, 1217

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.