pycom10g01440

Pleckstrin homology domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0009215
Physical Location & Seq
Forward (+)
1483934 .. 1489162
5229 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2634 bp
ATGGCAGATCTTGTTAGCTACGGGAATGCCAACCGTGACATCGACCAGGCAATAATTGCTCTAAAGAAGGGTGCTCAACTACTGAAATATGGTCGTAAGGGAAAGCCTAAGTTTTGTCCATTTAGACTTTCTACTGACGAATCATCTTTGATCTGGATTTCAAGTAGTGAAAGAAGTTTGAAGTTGGCTTCTGTCACAAGAATTGTTCCTGGACAAAGAACTGCTGTCTTTCAACGATATTTGCGTCCTGAAAAGGATTATTTATCCTTTTCTCTTATATACAATAACGGAAAGCGGTCTCTTGATCTGATTTGCAAGGACAAAGTTGAGGCAGAAGTGTGGATTGCAGGCCTCAAAGCACTAATTTCCTCTGGTAGTGGTGGGCGTTCCAAAATTGACGGATGGAGTGATGGAGGCCTCTACCTTGATGATGGCAGGGACTTGACATCAAATAGTCAAAGTGATAGTTCTGCTAGCGGTGCACGAGATAGTGGCTCTCCAGAGATTTCTGTTAGTTTCAAAACAAATTCACCCAAGAGTTTTCCGCCTGAAAATTCCCCGGTTTCTGAGAGGTCACATGCTGCATCAGACCAGACAAAGATGCAAGTAAAAGGATCGGGTTCAGATGCATTTCGAGTTAGTGTTTCAAGTGCTCCTAGCACTTCAAGTCATGGTTCAACACCGGATGATTGTGAAACTCTAGGTGATGTGTACATATGGGGTGAAGCTATTTGTGATAGTGTTGTTAAGATTGGGGCTGATAAAAATGCGAATTATTTGAGCCCGAGATCAGATGTGCTTGTCCCTAGGCCATTAGAGTCCAATCTTGTTTTGGATGTCCATCATATAGCCTGTGGGGTCAAACATGCAGCCTTGGTCACGAGACAAGGTGAAGTGTTTACATGGGGAGAAGAATCTGGAGGACGACTAGGGCATGGTGCTGGGAAGGATGTTGCTCAGCCTCGTCTAGTTGAATCTCTGGCAGCTACTGGTGTTGATTTTGTGGCATGTGGAGAATTTCATACTTGTGCTGTTACAATGGCTGGGGAACTTTATACATGGGGAGATGGCACGCATAATGCTGGACTTCTTGGTCATGGCACTGATGTCAGCCATTGGATACCAAAGAGAATTTCGGGTCCTCTTGAGGAACTTCAAGTTGCTTCTGTAACTTGTGGTCCATGGCATACAGCCTTGGTAACATCAACAGGGAAACTCTTCACATTTGGCGATGGCACATTTGGTGTCTTGGGCCATGGAGACAGAGAAAACATTCCATATCCGAGAGAAGTAGAATCCCTATCAGGGTTGAGGACTATATCTGTTGCATGTGGGGTGTGGCACACTGCTGCTGTGGTGGAGGTTATTGCAACACAATCTAGTGCAAGTAGTTCATCAGGTAAGTTGTTTACTTGGGGTGATGGAGATAAGAATCGACTTGGGCATGGAGACAAGGAAGCCCGGCTTGAACCGACATGTGTTCCTGCCCTTATCGACTACAATTTTCACAAAATTGCTTGTGGGCACAGTTTAACTGTAGGCTTGACAACGTCAGGACATGTTTTTACAATGGGCAGTACTGTTTATGGTCAGCTTGGAAATCCCAATTCTGATGGAAAGCTTCCTTGCTTGGTAGAAGACAAGCTTGCTGGGGATTGCGTTGAAGAAATTGCGTGTGGTGCGTATCATGTAGCAGTATTAACATCCAGGAATGAAGTTTATACTTGGGGAAAGGGAGCTAATGGGAGGTTGGGCCACGGGGATATTGAAGATCGGAAAACACCGACTCTCGTTGAAGCTTTAAAGGATAGACATGTGAAATATATTGGCTGTGGTCAAAACTATACAGCAGCTATTTGTCTTCACAAATGGGTATCTGGTGCTGAGCAGTCACAGTGCTCTGCTTGTAGACAGGCTTTTGGGTTCACAAAGAAGAGGCACAACTGCTATAATTGTGGACTTGTCCACTGCCATTCATGCAGTTCAAGAAAAGCAACAAGAGCAGCACTGGCTCCTAATCCTGGAAAGCCATATCGAGTTTGTGATGCTTGTTATGTGAAATTGAATAAGGTGTTAGAAACCGGTGGTAATAACAGAAGGAATGCGATACCTCGCTTGTCAGGTGAGAACAAGGACAGGTTGGACAAGGCTGACATAAGATTGTATAAGTCTGCTGTACTATCTAATATGGATTTGATAAAGCAATTAGATACTAAAGCAGCCAAGCAGGGAAAGAAAGCCGACACATTTTCACTTGTCCGCTCCGCTCAAGCACCTTCATTGTTGCAATTAAAGGATGTTGTTATGTCAACTGCTGTTGATTTGCGGCGAACAGTTCCCAAACCAGTTCTTACACCATCTGGAGCGAGTTCAAGGTCCGTGTCACCTTTCTCGAGGAGACCAAGCCCTCCTCGTTTTGCAACTCCTGTTCCTACTACATCAGGACTTTCCTTCTCAAAAAGTATTGCTGATAGTCTGAAGAAAACAAATGAACTTTTGAATCAGGAAGTGCTAAAATTACGTTCACAGGTTGAGAGCCTTAGACAGAGATGTGATCTATCAAGAATTAGAGCTTCAGAATTCTTCGAAGAAAGTACAAGAAGCAATGGCATTGGCCGCAGAGGAATCTGCTAA

Protein Analysis

878

Amino Acids

94.44

Weight (kDa)

8.9

Isoelectric Point (pI)

39.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PH_12 PF16457 16 - 122 3.3e-09 Pleckstrin homology domain
WD40_RLD PF25390 237 - 618 1.1e-78 RCC1-like domain
RCC1 PF00415 296 - 345 1.1e-10 Regulator of chromosome condensation (RCC1) repeat
Alsin_RLD PF25384 297 - 405 6.2e-07 Alsin RCC1-like domain
RCC1_2 PF13540 334 - 359 3.7e-06 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 349 - 400 7.8e-10 Regulator of chromosome condensation (RCC1) repeat
RCC1_2 PF13540 387 - 415 7.2e-06 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 404 - 452 2.5e-14 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 466 - 514 1.3e-11 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 518 - 567 4.8e-08 Regulator of chromosome condensation (RCC1) repeat
RCC1_2 PF13540 555 - 582 4.1e-07 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 572 - 619 2.1e-14 Regulator of chromosome condensation (RCC1) repeat
FYVE PF01363 623 - 689 5.1e-15 FYVE zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1092
AccBSI CCGCTC 2 cut(s) 2262, 2267
AccI GTMKAC 1 cut(s) 1909
AciI CCGC 7 cut(s) 295, 477, 545, 2260, 2265, 2326, 2617
AclWI GGATC 1 cut(s) 622
AcoI YGGCCR 1 cut(s) 2614
AcsI RAATTY 5 cut(s) 526, 553, 1016, 1131, 2579
AcuI CTGAAG 2 cut(s) 2498, 2559
AfaI GTAC 4 cut(s) 713, 1579, 2178, 2596
AfiI CCNNNNNNNGG 2 cut(s) 1305, 2021
AflIII ACRYGT 3 cut(s) 1475, 1558, 1813
AgeI ACCGGT 1 cut(s) 2081
AjnI CCWGG 4 cut(s) 45, 208, 1706, 2020
AjuI GAANNNNNNNTTGG 2 cut(s) 1117, 1149
Alw21I GWGCWC 4 cut(s) 76, 484, 655, 1901
Alw26I GTCTC 5 cut(s) 303, 877, 1254, 1443, 2392
Alw44I GTGCAC 1 cut(s) 480
AlwI GGATC 1 cut(s) 622
AlwNI CAGNNNCTG 1 cut(s) 989
Ama87I CYCGRG 2 cut(s) 784, 2392
AoxI GGCC 6 cut(s) 349, 415, 809, 1252, 1753, 2614
ApaLI GTGCAC 1 cut(s) 480
ApeKI GCWGC 7 cut(s) 581, 869, 983, 1349, 1850, 2003, 2219
ApoI RAATTY 5 cut(s) 526, 553, 1016, 1131, 2579
AsiGI ACCGGT 1 cut(s) 2081
Asp700I GAANNNNTTC 2 cut(s) 1217, 1272
AspA2I CCTAGG 1 cut(s) 806
AspS9I GGNCC 5 cut(s) 1139, 1178, 1252, 1753, 2376
AsuC2I CCSGG 2 cut(s) 560, 1462
AsuHPI GGTGA 7 cut(s) 522, 716, 734, 902, 1430, 2135, 2376
AsuII TTCGAA 1 cut(s) 2586
AsuNHI GCTAGC 1 cut(s) 473
AvaI CYCGRG 2 cut(s) 784, 2392
AvaII GGWCC 3 cut(s) 1139, 1178, 2376
AvrII CCTAGG 1 cut(s) 806
BaeGI GKGCMC 2 cut(s) 484, 1527
BanII GRGCYC 1 cut(s) 785
BauI CACGAG 2 cut(s) 483, 880
BbsI GAAGAC 2 cut(s) 1644, 1853
Bbv12I GWGCWC 4 cut(s) 76, 484, 655, 1901
BbvI GCAGC 7 cut(s) 568, 881, 995, 1336, 1862, 2015, 2231
BccI CCATC 9 cut(s) 396, 404, 425, 849, 1061, 1226, 1415, 1607, 2365
BciT130I CCWGG 4 cut(s) 47, 210, 1708, 2022
BciVI GTATCC 1 cut(s) 1113
BcnI CCSGG 2 cut(s) 560, 1462
BcoDI GTCTC 5 cut(s) 303, 877, 1254, 1443, 2392
BfaI CTAG 7 cut(s) 474, 657, 701, 807, 929, 968, 1380
BfmI CTRYAG 1 cut(s) 1536
BfuI GTATCC 1 cut(s) 1113
BglII AGATCT 1 cut(s) 7
BisI GCNGC 9 cut(s) 582, 870, 984, 1350, 1851, 2004, 2220, 2327, 2617
BlnI CCTAGG 1 cut(s) 806
BlpI GCTNAGC 2 cut(s) 957, 1884
BlsI GCNGC 9 cut(s) 583, 871, 985, 1351, 1852, 2005, 2221, 2328, 2618
BmcAI AGTACT 1 cut(s) 1579
Bme1390I CCNGG 6 cut(s) 47, 210, 560, 1462, 1708, 2022
Bme18I GGWCC 3 cut(s) 1139, 1178, 2376
BmeT110I CYCGRG 2 cut(s) 784, 2392
BmgT120I GGNCC 5 cut(s) 1139, 1178, 1252, 1753, 2376
BmiI GGNNCC 2 cut(s) 1140, 2013
BmrFI CCNGG 6 cut(s) 47, 210, 560, 1462, 1708, 2022
BmsI GCATC 4 cut(s) 591, 593, 616, 2035
BmtI GCTAGC 1 cut(s) 477
BpiI GAAGAC 2 cut(s) 1644, 1853
BpmI CTGGAG 3 cut(s) 483, 939, 2382
Bpu1102I GCTNAGC 2 cut(s) 957, 1884
Bpu14I TTCGAA 1 cut(s) 2586
BpuEI CTTGAG 2 cut(s) 1166, 2253
BpuMI CCSGG 2 cut(s) 560, 1462
BsaBI GATNNNNATC 2 cut(s) 840, 1431
BsaI GGTCTC 2 cut(s) 303, 2392
BsaJI CCNNGG 7 cut(s) 558, 806, 873, 1181, 1194, 1255, 1756
BsaWI WCCGGW 2 cut(s) 682, 2081
Bsc4I CCNNNNNNNGG 2 cut(s) 1305, 2021
Bse118I RCCGGY 1 cut(s) 2081
Bse1I ACTGG 3 cut(s) 994, 2013, 2345
Bse3DI GCAATG 1 cut(s) 2611
Bse8I GATNNNNATC 2 cut(s) 840, 1431
BseBI CCWGG 4 cut(s) 47, 210, 1708, 2022
BseDI CCNNGG 7 cut(s) 558, 806, 873, 1181, 1194, 1255, 1756
BseGI GGATG 6 cut(s) 407, 691, 841, 955, 1703, 2302
BseJI GATNNNNATC 2 cut(s) 840, 1431
BseLI CCNNNNNNNGG 2 cut(s) 1305, 2021
BseMI GCAATG 1 cut(s) 2611
BseMII CTCAG 3 cut(s) 558, 971, 1875
BseNI ACTGG 3 cut(s) 994, 2013, 2345
BseRI GAGGAG 2 cut(s) 2400, 2410
BseSI GKGCMC 2 cut(s) 484, 1527
BseXI GCAGC 7 cut(s) 568, 881, 995, 1336, 1862, 2015, 2231
BseYI CCCAGC 3 cut(s) 941, 1043, 1649
BshFI GGCC 6 cut(s) 351, 417, 811, 1254, 1755, 2616
BshTI ACCGGT 1 cut(s) 2081
BsiHKAI GWGCWC 4 cut(s) 76, 484, 655, 1901
BsiHKCI CYCGRG 2 cut(s) 784, 2392
BsiSI CCGG 4 cut(s) 560, 683, 1462, 2082
BslFI GGGAC 2 cut(s) 452, 788
BslI CCNNNNNNNGG 2 cut(s) 1305, 2021
BsmAI GTCTC 5 cut(s) 303, 877, 1254, 1443, 2392
BsmFI GGGAC 2 cut(s) 452, 788
BsmI GAATGC 2 cut(s) 31, 2107
BsnI GGCC 6 cut(s) 351, 417, 811, 1254, 1755, 2616
Bso31I GGTCTC 2 cut(s) 303, 2392
BsoBI CYCGRG 2 cut(s) 784, 2392
Bsp119I TTCGAA 1 cut(s) 2586
Bsp1286I GDGCHC 6 cut(s) 76, 484, 655, 785, 1527, 1901
Bsp1407I TGTACA 1 cut(s) 711
Bsp143I GATC 7 cut(s) 7, 150, 304, 614, 788, 1771, 2554
Bsp1720I GCTNAGC 2 cut(s) 957, 1884
Bsp19I CCATGG 2 cut(s) 1181, 1255
BspACI CCGC 7 cut(s) 295, 477, 545, 2260, 2265, 2326, 2617
BspANI GGCC 6 cut(s) 351, 417, 811, 1254, 1755, 2616
BspCNI CTCAG 3 cut(s) 559, 970, 1876
BspLI GGNNCC 2 cut(s) 1140, 2013
BspOI GCTAGC 1 cut(s) 477
BspPI GGATC 1 cut(s) 622
BspT104I TTCGAA 1 cut(s) 2586
BspTNI GGTCTC 2 cut(s) 303, 2392
BsrBI CCGCTC 2 cut(s) 2262, 2267
BsrDI GCAATG 1 cut(s) 2611
BsrFI RCCGGY 1 cut(s) 2081
BsrGI TGTACA 1 cut(s) 711
BsrI ACTGG 3 cut(s) 994, 2013, 2345
BssAI RCCGGY 1 cut(s) 2081
BssECI CCNNGG 7 cut(s) 558, 806, 873, 1181, 1194, 1255, 1756
BssMI GATC 7 cut(s) 7, 150, 304, 614, 788, 1771, 2554
BssSI CACGAG 2 cut(s) 483, 880
BssT1I CCWWGG 5 cut(s) 806, 873, 1181, 1194, 1255
Bst2BI CACGAG 2 cut(s) 483, 880
Bst2UI CCWGG 4 cut(s) 47, 210, 1708, 2022
Bst4CI ACNGT 6 cut(s) 35, 1529, 1537, 1582, 1896, 2335
Bst6I CTCTTC 2 cut(s) 1223, 1928
BstAPI GCANNNNNTGC 1 cut(s) 56
BstAUI TGTACA 1 cut(s) 711
BstBI TTCGAA 1 cut(s) 2586
BstC8I GCNNGC 4 cut(s) 349, 475, 1073, 1647
BstDEI CTNAG 5 cut(s) 108, 567, 957, 1884, 2540
BstDSI CCRYGG 3 cut(s) 1181, 1255, 1756
BstF5I GGATG 6 cut(s) 407, 691, 841, 955, 1703, 2302
BstKTI GATC 7 cut(s) 10, 153, 307, 617, 791, 1774, 2557
BstMAI GTCTC 5 cut(s) 303, 877, 1254, 1443, 2392
BstMBI GATC 7 cut(s) 7, 150, 304, 614, 788, 1771, 2554
BstMWI GCNNNNNNNGC 7 cut(s) 56, 479, 1679, 1977, 2000, 2009, 2616
BstNI CCWGG 4 cut(s) 47, 210, 1708, 2022
BstNSI RCATGY 7 cut(s) 581, 869, 1011, 1332, 1479, 1562, 1817
BstSCI CCNGG 6 cut(s) 45, 208, 558, 1460, 1706, 2020
BstSFI CTRYAG 1 cut(s) 1536
BstSLI GKGCMC 2 cut(s) 484, 1527
BstV1I GCAGC 7 cut(s) 568, 881, 995, 1336, 1862, 2015, 2231
BstV2I GAAGAC 2 cut(s) 1644, 1853
BstX2I RGATCY 1 cut(s) 7
BstYI RGATCY 1 cut(s) 7
BsuI GTATCC 1 cut(s) 1113
BsuRI GGCC 6 cut(s) 351, 417, 811, 1254, 1755, 2616
BtgI CCRYGG 3 cut(s) 1181, 1255, 1756
BtgZI GCGATG 1 cut(s) 1245
BtsCI GGATG 6 cut(s) 407, 691, 841, 955, 1703, 2302
BtsI GCAGTG 2 cut(s) 1344, 1966
BtsIMutI CAGTG 5 cut(s) 1101, 1344, 1901, 1966, 2006
Cac8I GCNNGC 4 cut(s) 349, 475, 1073, 1647
CaiI CAGNNNCTG 1 cut(s) 989
Cfr10I RCCGGY 1 cut(s) 2081
Cfr13I GGNCC 5 cut(s) 1139, 1178, 1252, 1753, 2376
CseI GACGC 1 cut(s) 233
Csp6I GTAC 4 cut(s) 712, 1578, 2177, 2595
CspAI ACCGGT 1 cut(s) 2081
CviQI GTAC 4 cut(s) 712, 1578, 2177, 2595
DdeI CTNAG 5 cut(s) 108, 567, 957, 1884, 2540
DpnI GATC 7 cut(s) 9, 152, 306, 616, 790, 1773, 2556
DpnII GATC 7 cut(s) 7, 150, 304, 614, 788, 1771, 2554
DraI TTTAAA 1 cut(s) 1803
DrdI GACNNNNNNGTC 1 cut(s) 1092
DseDI GACNNNNNNGTC 1 cut(s) 1092
EaeI YGGCCR 1 cut(s) 2614
Eam1104I CTCTTC 2 cut(s) 1223, 1928
EarI CTCTTC 2 cut(s) 1223, 1928
EciI GGCGGA 1 cut(s) 534
Eco130I CCWWGG 5 cut(s) 806, 873, 1181, 1194, 1255
Eco147I AGGCCT 2 cut(s) 351, 417
Eco24I GRGCYC 1 cut(s) 785
Eco31I GGTCTC 2 cut(s) 303, 2392
Eco47I GGWCC 3 cut(s) 1139, 1178, 2376
Eco57I CTGAAG 2 cut(s) 2498, 2559
Eco88I CYCGRG 2 cut(s) 784, 2392
EcoO109I RGGNCCY 1 cut(s) 1139
EcoRI GAATTC 1 cut(s) 2579
EcoRII CCWGG 4 cut(s) 45, 208, 1706, 2020
EcoT14I CCWWGG 5 cut(s) 806, 873, 1181, 1194, 1255
EcoT22I ATGCAT 1 cut(s) 631
EcoT38I GRGCYC 1 cut(s) 785
ErhI CCWWGG 5 cut(s) 806, 873, 1181, 1194, 1255
FalI AAGNNNNNCTT 4 cut(s) 1449, 1481, 1629, 1661
FaqI GGGAC 2 cut(s) 452, 788
FauNDI CATATG 1 cut(s) 716
FblI GTMKAC 1 cut(s) 1909
Fnu4HI GCNGC 9 cut(s) 582, 870, 984, 1350, 1851, 2004, 2220, 2327, 2617
FokI GGATG 6 cut(s) 414, 698, 848, 962, 1690, 2309
FriOI GRGCYC 1 cut(s) 785
Fsp4HI GCNGC 9 cut(s) 582, 870, 984, 1350, 1851, 2004, 2220, 2327, 2617
FspBI CTAG 7 cut(s) 474, 657, 701, 807, 929, 968, 1380
GluI GCNGC 9 cut(s) 582, 870, 984, 1350, 1851, 2004, 2220, 2327, 2617
GsaI CCCAGC 3 cut(s) 945, 1047, 1653
GsuI CTGGAG 3 cut(s) 483, 939, 2382
HaeIII GGCC 6 cut(s) 351, 417, 811, 1254, 1755, 2616
HapII CCGG 4 cut(s) 560, 683, 1462, 2082
HgaI GACGC 1 cut(s) 233
HincII GTYRAC 1 cut(s) 2310
HindII GTYRAC 1 cut(s) 2310
HindIII AAGCTT 3 cut(s) 1619, 1643, 1797
HinfI GANTC 9 cut(s) 140, 818, 914, 974, 1295, 1432, 1786, 2500, 2625
HpaII CCGG 4 cut(s) 560, 683, 1462, 2082
HphI GGTGA 7 cut(s) 522, 716, 734, 902, 1430, 2135, 2376
HpyAV CCTTC 5 cut(s) 61, 940, 2091, 2286, 2461
HpyCH4III ACNGT 6 cut(s) 35, 1529, 1537, 1582, 1896, 2335
HpyCH4IV ACGT 2 cut(s) 1550, 2521
HpyF10VI GCNNNNNNNGC 7 cut(s) 56, 479, 1679, 1977, 2000, 2009, 2616
HpyF3I CTNAG 5 cut(s) 108, 567, 957, 1884, 2540
HpySE526I ACGT 2 cut(s) 1550, 2521
Kzo9I GATC 7 cut(s) 7, 150, 304, 614, 788, 1771, 2554
LmnI GCTCC 5 cut(s) 658, 1736, 2017, 2267, 2363
Lsp1109I GCAGC 7 cut(s) 568, 881, 995, 1336, 1862, 2015, 2231
LweI GCATC 4 cut(s) 591, 593, 616, 2035
MaeI CTAG 7 cut(s) 474, 657, 701, 807, 929, 968, 1380
MaeII ACGT 2 cut(s) 1550, 2521
MaeIII GTNAC 9 cut(s) 35, 193, 573, 877, 1033, 1168, 1198, 1890, 2382
MalI GATC 7 cut(s) 9, 152, 306, 616, 790, 1773, 2556
MbiI CCGCTC 2 cut(s) 2262, 2267
MboI GATC 7 cut(s) 7, 150, 304, 614, 788, 1771, 2554
MflI RGATCY 1 cut(s) 7
MhlI GDGCHC 6 cut(s) 76, 484, 655, 785, 1527, 1901
MlyI GAGTC 2 cut(s) 827, 1780
MmeI TCCRAC 1 cut(s) 2121
Mph1103I ATGCAT 1 cut(s) 631
MroXI GAANNNNTTC 2 cut(s) 1217, 1272
MseI TTAA 5 cut(s) 747, 1532, 1700, 1802, 2291
MslI CAYNNNNRTG 1 cut(s) 1026
MspI CCGG 4 cut(s) 560, 683, 1462, 2082
MspR9I CCNGG 6 cut(s) 47, 210, 560, 1462, 1708, 2022
Mva1269I GAATGC 2 cut(s) 31, 2107
MvaI CCWGG 4 cut(s) 47, 210, 1708, 2022
MwoI GCNNNNNNNGC 7 cut(s) 56, 479, 1679, 1977, 2000, 2009, 2616
NciI CCSGG 2 cut(s) 560, 1462
NcoI CCATGG 2 cut(s) 1181, 1255
NdeI CATATG 1 cut(s) 716
NdeII GATC 7 cut(s) 7, 150, 304, 614, 788, 1771, 2554
NheI GCTAGC 1 cut(s) 473
NlaIV GGNNCC 2 cut(s) 1140, 2013
NmuCI GTSAC 6 cut(s) 35, 193, 573, 877, 1890, 2382
NsiI ATGCAT 1 cut(s) 631
NspI RCATGY 7 cut(s) 581, 869, 1011, 1332, 1479, 1562, 1817
NspV TTCGAA 1 cut(s) 2586
PaeR7I CTCGAG 1 cut(s) 2392
PceI AGGCCT 2 cut(s) 351, 417
PciI ACATGT 3 cut(s) 1475, 1558, 1813
PcsI WCGNNNNNNNCGW 1 cut(s) 241
PctI GAATGC 2 cut(s) 31, 2107
PdmI GAANNNNTTC 2 cut(s) 1217, 1272
PfeI GAWTC 7 cut(s) 140, 914, 974, 1295, 1432, 2500, 2625
PflFI GACNNNGTC 1 cut(s) 1549
PfoI TCCNGGA 3 cut(s) 208, 1706, 2020
PinAI ACCGGT 1 cut(s) 2081
PkrI GCNGC 9 cut(s) 583, 871, 985, 1351, 1852, 2005, 2221, 2328, 2618
PleI GAGTC 2 cut(s) 826, 1780
PpsI GAGTC 2 cut(s) 826, 1780
PpuMI RGGWCCY 1 cut(s) 1139
PscI ACATGT 3 cut(s) 1475, 1558, 1813
Psp5II RGGWCCY 1 cut(s) 1139
Psp6I CCWGG 4 cut(s) 45, 208, 1706, 2020
PspFI CCCAGC 3 cut(s) 941, 1043, 1649
PspGI CCWGG 4 cut(s) 45, 208, 1706, 2020
PspN4I GGNNCC 2 cut(s) 1140, 2013
PspPI GGNCC 5 cut(s) 1139, 1178, 1252, 1753, 2376
PspPPI RGGWCCY 1 cut(s) 1139
PstNI CAGNNNCTG 1 cut(s) 989
PsuI RGATCY 1 cut(s) 7
PsyI GACNNNGTC 1 cut(s) 1549
RsaI GTAC 4 cut(s) 713, 1579, 2178, 2596
RsaNI GTAC 4 cut(s) 712, 1578, 2177, 2595
RseI CAYNNNNRTG 1 cut(s) 1026
SaqAI TTAA 5 cut(s) 747, 1532, 1700, 1802, 2291
SatI GCNGC 9 cut(s) 582, 870, 984, 1350, 1851, 2004, 2220, 2327, 2617
Sau3AI GATC 7 cut(s) 7, 150, 304, 614, 788, 1771, 2554
Sau96I GGNCC 5 cut(s) 1139, 1178, 1252, 1753, 2376
ScaI AGTACT 1 cut(s) 1579
SchI GAGTC 2 cut(s) 827, 1780
ScrFI CCNGG 6 cut(s) 47, 210, 560, 1462, 1708, 2022
SduI GDGCHC 6 cut(s) 76, 484, 655, 785, 1527, 1901
SfaNI GCATC 4 cut(s) 591, 593, 616, 2035
SfcI CTRYAG 1 cut(s) 1536
Sfr274I CTCGAG 1 cut(s) 2392
SfuI TTCGAA 1 cut(s) 2586
SinI GGWCC 3 cut(s) 1139, 1178, 2376
SlaI CTCGAG 1 cut(s) 2392
SmiMI CAYNNNNRTG 1 cut(s) 1026
SmlI CTYRAG 3 cut(s) 1145, 2268, 2392
SmoI CTYRAG 3 cut(s) 1145, 2268, 2392
SseBI AGGCCT 2 cut(s) 351, 417
SsiI CCGC 7 cut(s) 295, 477, 545, 2260, 2265, 2326, 2617
SspMI CTAG 7 cut(s) 474, 657, 701, 807, 929, 968, 1380
StuI AGGCCT 2 cut(s) 351, 417
StyD4I CCNGG 6 cut(s) 45, 208, 558, 1460, 1706, 2020
StyI CCWWGG 5 cut(s) 806, 873, 1181, 1194, 1255
TaaI ACNGT 6 cut(s) 35, 1529, 1537, 1582, 1896, 2335
TaiI ACGT 2 cut(s) 1553, 2524
TaqI TCGA 7 cut(s) 42, 634, 1435, 1494, 2035, 2393, 2586
TatI WGTACW 4 cut(s) 711, 1577, 2176, 2594
TauI GCSGC 2 cut(s) 2329, 2619
TfiI GAWTC 7 cut(s) 140, 914, 974, 1295, 1432, 2500, 2625
Tru1I TTAA 5 cut(s) 747, 1532, 1700, 1802, 2291
Tru9I TTAA 5 cut(s) 747, 1532, 1700, 1802, 2291
TscAI CASTG 5 cut(s) 1108, 1351, 1901, 1973, 2013
TseFI GTSAC 6 cut(s) 35, 193, 573, 877, 1890, 2382
TseI GCWGC 7 cut(s) 581, 869, 983, 1349, 1850, 2003, 2219
Tsp45I GTSAC 6 cut(s) 35, 193, 573, 877, 1890, 2382
TspDTI ATGAA 6 cut(s) 1010, 1383, 1728, 1965, 2268, 2505
TspGWI ACGGA 3 cut(s) 303, 414, 2368
TspRI CASTG 5 cut(s) 1108, 1351, 1901, 1973, 2013
Tth111I GACNNNGTC 1 cut(s) 1549
VneI GTGCAC 1 cut(s) 480
VpaK11BI GGWCC 3 cut(s) 1139, 1178, 2376
XapI RAATTY 5 cut(s) 526, 553, 1016, 1131, 2579
XceI RCATGY 7 cut(s) 581, 869, 1011, 1332, 1479, 1562, 1817
XcmI CCANNNNNNNNNTGG 1 cut(s) 829
XhoI CTCGAG 1 cut(s) 2392
XmaJI CCTAGG 1 cut(s) 806
XmiI GTMKAC 1 cut(s) 1909
XmnI GAANNNNTTC 2 cut(s) 1217, 1272
XspI CTAG 7 cut(s) 474, 657, 701, 807, 929, 968, 1380
ZrmI AGTACT 1 cut(s) 1579
Zsp2I ATGCAT 1 cut(s) 631
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.