pycom10g04780

Protein BASIC PENTACYSTEINE4-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0005753
Physical Location & Seq
Reverse (-)
5031790 .. 5034691
2902 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1014 bp
ATGGTATACGGTCAGCAGATAGCAGGAGAGGAGGAAAGGTGGTTAAAATTTTCAGCACTTGCTGCTAATATTTCGAAAATGGATGATGGTCGGCAACATGAAAATGGGAGACACAAGATGGACTATTACAGAGGGGGGGTGGCATCGCCGTGGAATATGATGACCCAACAGCAAGCGAAGGAACCAAATGCCTTAGTCATGAACAAGAAGATCATGTCCATTATTGCTGACAGGGATGCTGCTATTCGAGAACGGAATGCAGCACTTGCTGAAAAGAATGAAGCCTTGGCTGCAAGAGATGAGGCACTCCGTCAGCGAGATGAGGCACTAGCTCAGCGTGATAGTGCCCTGATGGAACGGGACAACGCCTATGCAGCCCTTCATTCGCGGGATAATGCTGTCAACTTCCCGTTGGGTGGTGGAGCTCAACGTGGATCTAAACGCTTGCAGCGCCCCTCAAACCATTCAGTTACCCTGGCTGATGTTCATTACAGCACAAAAGATGTGCATGTAACTGAGGCCTACCCTATTTCAGTTATATCTCCTGAAGCTGTCAAGTCACGTCAGACAAAGCGAGCAAAGGAGAACAAGGCATCCAGGGCAAAGCAGTCACGGAAAAAAGTAGGCGAAGATTTGAATAGGCAGGCTTCCTCGGATGGGATTAAGTATAAATCAGAGTGGGATACTCATGATTTGGGTTTGAATCTTGTCTCTTTCGACGAGTCTACAATGCCAGTGCCAGTTTGTTCATGCACTGGAATTCCGCGGCAGTGCTACAAATGGGGGAATGGCGGGTGGCAGTCATCTTGTTGCACCACCCATATGTCTATGTATCCACTACCTCAGATGCCAAATAAGCGCCATGCCCGGATGGGTGGGCGGAAGATGAGCGGAAGTGTTTTTACTAGATTGCTTAGTCGGCTAGGAGCAGATGGTCATGATTTGTCAATACCGCTGGATTTGAAAGAATACTGGGCCAGACATGGGACAAATCGCTACATAACGATCAAGTAG

Protein Analysis

338

Amino Acids

37.85

Weight (kDa)

9.56

Isoelectric Point (pI)

55.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GAGA_bind PF06217 27 - 337 1e-114 GAGA binding protein-like family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 891
AccI GTMKAC 2 cut(s) 6, 725
AccII CGCG 2 cut(s) 388, 766
AciI CCGC 7 cut(s) 388, 764, 766, 792, 880, 891, 953
AclWI GGATC 1 cut(s) 442
AcsI RAATTY 2 cut(s) 47, 759
AcuI CTGAAG 1 cut(s) 567
AfiI CCNNNNNNNGG 3 cut(s) 416, 657, 984
AgsI TTSAA 3 cut(s) 637, 703, 964
AjiI CACGTC 1 cut(s) 563
AjnI CCWGG 2 cut(s) 474, 596
AjuI GAANNNNNNNTTGG 2 cut(s) 269, 301
AloI GAACNNNNNNTCC 2 cut(s) 578, 610
AluBI AGCT 3 cut(s) 332, 425, 551
AluI AGCT 3 cut(s) 332, 425, 551
Alw21I GWGCWC 1 cut(s) 427
Alw26I GTCTC 2 cut(s) 103, 715
AlwI GGATC 1 cut(s) 442
AoxI GGCC 2 cut(s) 519, 975
ApeKI GCWGC 6 cut(s) 62, 239, 260, 290, 374, 448
ApoI RAATTY 2 cut(s) 47, 759
AspLEI GCGC 2 cut(s) 453, 861
AspS9I GGNCC 1 cut(s) 975
AsuC2I CCSGG 1 cut(s) 868
AsuII TTCGAA 1 cut(s) 74
BaeGI GKGCMC 1 cut(s) 349
BanII GRGCYC 1 cut(s) 427
BarI GAAGNNNNNNTAC 2 cut(s) 886, 918
Bbv12I GWGCWC 1 cut(s) 427
BbvI GCAGC 6 cut(s) 49, 226, 272, 277, 386, 460
BccI CCATC 6 cut(s) 80, 112, 346, 650, 865, 926
BceAI ACGGC 1 cut(s) 133
BciT130I CCWGG 2 cut(s) 476, 598
BciVI GTATCC 2 cut(s) 676, 843
BcnI CCSGG 1 cut(s) 868
BcoDI GTCTC 2 cut(s) 103, 715
BfaI CTAG 3 cut(s) 329, 906, 923
BfoI RGCGCY 2 cut(s) 454, 862
BfuI GTATCC 2 cut(s) 676, 843
BisI GCNGC 7 cut(s) 63, 240, 261, 291, 375, 449, 767
BlpI GCTNAGC 1 cut(s) 333
BlsI GCNGC 7 cut(s) 64, 241, 262, 292, 376, 450, 768
Bme1390I CCNGG 3 cut(s) 476, 598, 868
BmgBI CACGTC 1 cut(s) 563
BmgT120I GGNCC 1 cut(s) 975
BmiI GGNNCC 1 cut(s) 183
BmrFI CCNGG 3 cut(s) 476, 598, 868
BmrI ACTGGG 1 cut(s) 982
BmsI GCATC 4 cut(s) 152, 226, 602, 837
BmuI ACTGGG 1 cut(s) 982
Bpu1102I GCTNAGC 1 cut(s) 333
Bpu14I TTCGAA 1 cut(s) 74
BpuMI CCSGG 1 cut(s) 868
BsaJI CCNNGG 6 cut(s) 149, 285, 474, 597, 651, 764
BsaXI ACNNNNNCTCC 2 cut(s) 23, 53
Bsc4I CCNNNNNNNGG 3 cut(s) 416, 657, 984
Bse1I ACTGG 4 cut(s) 734, 740, 760, 977
BseBI CCWGG 2 cut(s) 476, 598
BseDI CCNNGG 6 cut(s) 149, 285, 474, 597, 651, 764
BseGI GGATG 5 cut(s) 88, 241, 593, 661, 876
BseLI CCNNNNNNNGG 3 cut(s) 416, 657, 984
BseMII CTCAG 3 cut(s) 347, 507, 857
BseNI ACTGG 4 cut(s) 734, 740, 760, 977
BseRI GAGGAG 1 cut(s) 44
BseSI GKGCMC 1 cut(s) 349
BseXI GCAGC 6 cut(s) 49, 226, 272, 277, 386, 460
Bsh1236I CGCG 2 cut(s) 388, 766
BshFI GGCC 2 cut(s) 521, 977
BsiHKAI GWGCWC 1 cut(s) 427
BsiSI CCGG 1 cut(s) 868
BslFI GGGAC 2 cut(s) 374, 1000
BslI CCNNNNNNNGG 3 cut(s) 416, 657, 984
BsmAI GTCTC 2 cut(s) 103, 715
BsmFI GGGAC 2 cut(s) 374, 1000
BsmI GAATGC 1 cut(s) 262
BsnI GGCC 2 cut(s) 521, 977
Bsp119I TTCGAA 1 cut(s) 74
Bsp1286I GDGCHC 2 cut(s) 349, 427
Bsp143I GATC 3 cut(s) 210, 434, 1005
Bsp1720I GCTNAGC 1 cut(s) 333
BspACI CCGC 7 cut(s) 388, 764, 766, 792, 880, 891, 953
BspANI GGCC 2 cut(s) 521, 977
BspCNI CTCAG 3 cut(s) 346, 508, 856
BspFNI CGCG 2 cut(s) 388, 766
BspHI TCATGA 3 cut(s) 198, 688, 937
BspLI GGNNCC 1 cut(s) 183
BspPI GGATC 1 cut(s) 442
BspT104I TTCGAA 1 cut(s) 74
BsrBI CCGCTC 1 cut(s) 891
BsrI ACTGG 4 cut(s) 734, 740, 760, 977
BssECI CCNNGG 6 cut(s) 149, 285, 474, 597, 651, 764
BssMI GATC 3 cut(s) 210, 434, 1005
BssNAI GTATAC 1 cut(s) 7
BssT1I CCWWGG 1 cut(s) 285
Bst1107I GTATAC 1 cut(s) 7
Bst2UI CCWGG 2 cut(s) 476, 598
Bst4CI ACNGT 1 cut(s) 11
BstAPI GCANNNNNTGC 2 cut(s) 62, 266
BstBI TTCGAA 1 cut(s) 74
BstC8I GCNNGC 4 cut(s) 174, 446, 576, 645
BstDEI CTNAG 5 cut(s) 193, 333, 516, 843, 914
BstDSI CCRYGG 2 cut(s) 149, 764
BstF5I GGATG 5 cut(s) 88, 241, 593, 661, 876
BstFNI CGCG 2 cut(s) 388, 766
BstH2I RGCGCY 2 cut(s) 454, 862
BstHHI GCGC 2 cut(s) 453, 861
BstKTI GATC 3 cut(s) 213, 437, 1008
BstMAI GTCTC 2 cut(s) 103, 715
BstMBI GATC 3 cut(s) 210, 434, 1005
BstMWI GCNNNNNNNGC 8 cut(s) 62, 266, 290, 374, 450, 599, 856, 919
BstNI CCWGG 2 cut(s) 476, 598
BstNSI RCATGY 1 cut(s) 512
BstSCI CCNGG 3 cut(s) 474, 596, 866
BstSLI GKGCMC 1 cut(s) 349
BstUI CGCG 2 cut(s) 388, 766
BstV1I GCAGC 6 cut(s) 49, 226, 272, 277, 386, 460
BstX2I RGATCY 1 cut(s) 434
BstYI RGATCY 1 cut(s) 434
BstZ17I GTATAC 1 cut(s) 7
BsuI GTATCC 2 cut(s) 676, 843
BsuRI GGCC 2 cut(s) 521, 977
BtgI CCRYGG 2 cut(s) 149, 764
BtgZI GCGATG 1 cut(s) 129
BtrI CACGTC 1 cut(s) 563
BtsCI GGATG 5 cut(s) 88, 241, 593, 661, 876
BtsI GCAGTG 1 cut(s) 776
BtsIMutI CAGTG 3 cut(s) 741, 753, 776
Cac8I GCNNGC 4 cut(s) 174, 446, 576, 645
CciI TCATGA 3 cut(s) 198, 688, 937
CfoI GCGC 2 cut(s) 453, 861
Cfr13I GGNCC 1 cut(s) 975
Cfr42I CCGCGG 1 cut(s) 767
CviAII CATG 9 cut(s) 98, 199, 214, 509, 689, 750, 863, 938, 983
DdeI CTNAG 5 cut(s) 193, 333, 516, 843, 914
DpnI GATC 3 cut(s) 212, 436, 1007
DpnII GATC 3 cut(s) 210, 434, 1005
EciI GGCGGA 1 cut(s) 895
Ecl136II GAGCTC 1 cut(s) 425
Eco130I CCWWGG 1 cut(s) 285
Eco147I AGGCCT 1 cut(s) 521
Eco24I GRGCYC 1 cut(s) 427
Eco53kI GAGCTC 1 cut(s) 425
Eco57I CTGAAG 1 cut(s) 567
EcoICRI GAGCTC 1 cut(s) 425
EcoRI GAATTC 1 cut(s) 759
EcoRII CCWGG 2 cut(s) 474, 596
EcoT14I CCWWGG 1 cut(s) 285
EcoT38I GRGCYC 1 cut(s) 427
ErhI CCWWGG 1 cut(s) 285
FaeI CATG 9 cut(s) 101, 202, 217, 512, 692, 753, 866, 941, 986
FaqI GGGAC 2 cut(s) 374, 1000
FatI CATG 9 cut(s) 97, 198, 213, 508, 688, 749, 862, 937, 982
FauI CCCGC 2 cut(s) 381, 785
FauNDI CATATG 1 cut(s) 822
FblI GTMKAC 2 cut(s) 6, 725
Fnu4HI GCNGC 7 cut(s) 63, 240, 261, 291, 375, 449, 767
FokI GGATG 5 cut(s) 95, 248, 580, 668, 883
FriOI GRGCYC 1 cut(s) 427
Fsp4HI GCNGC 7 cut(s) 63, 240, 261, 291, 375, 449, 767
FspBI CTAG 3 cut(s) 329, 906, 923
GlaI GCGC 2 cut(s) 452, 860
GluI GCNGC 7 cut(s) 63, 240, 261, 291, 375, 449, 767
HaeII RGCGCY 2 cut(s) 454, 862
HaeIII GGCC 2 cut(s) 521, 977
HapII CCGG 1 cut(s) 868
HhaI GCGC 2 cut(s) 453, 861
Hin1II CATG 9 cut(s) 101, 202, 217, 512, 692, 753, 866, 941, 986
Hin6I GCGC 2 cut(s) 451, 859
HinP1I GCGC 2 cut(s) 451, 859
HincII GTYRAC 1 cut(s) 403
HindII GTYRAC 1 cut(s) 403
HinfI GANTC 2 cut(s) 703, 722
HpaII CCGG 1 cut(s) 868
Hpy166II GTNNAC 3 cut(s) 7, 403, 726
Hpy188I TCNGA 4 cut(s) 567, 655, 676, 846
Hpy188III TCNNGA 5 cut(s) 199, 248, 545, 689, 938
Hpy8I GTNNAC 3 cut(s) 7, 403, 726
Hpy99I CGWCG 1 cut(s) 722
HpyAV CCTTC 2 cut(s) 172, 389
HpyCH4III ACNGT 1 cut(s) 11
HpyCH4IV ACGT 2 cut(s) 430, 562
HpyCH4V TGCA 7 cut(s) 260, 293, 374, 448, 508, 753, 813
HpyF10VI GCNNNNNNNGC 8 cut(s) 62, 266, 290, 374, 450, 599, 856, 919
HpyF3I CTNAG 5 cut(s) 193, 333, 516, 843, 914
HpySE526I ACGT 2 cut(s) 430, 562
Hsp92II CATG 9 cut(s) 101, 202, 217, 512, 692, 753, 866, 941, 986
HspAI GCGC 2 cut(s) 451, 859
KspI CCGCGG 1 cut(s) 767
Kzo9I GATC 3 cut(s) 210, 434, 1005
LmnI GCTCC 2 cut(s) 422, 926
Lsp1109I GCAGC 6 cut(s) 49, 226, 272, 277, 386, 460
LweI GCATC 4 cut(s) 152, 226, 602, 837
MaeI CTAG 3 cut(s) 329, 906, 923
MaeII ACGT 2 cut(s) 430, 562
MaeIII GTNAC 4 cut(s) 469, 511, 558, 609
MalI GATC 3 cut(s) 212, 436, 1007
MbiI CCGCTC 1 cut(s) 891
MboI GATC 3 cut(s) 210, 434, 1005
MboII GAAGA 3 cut(s) 220, 641, 895
MflI RGATCY 1 cut(s) 434
MhlI GDGCHC 2 cut(s) 349, 427
MluCI AATT 2 cut(s) 47, 759
MlyI GAGTC 1 cut(s) 731
MnlI CCTC 9 cut(s) 22, 25, 125, 295, 316, 466, 511, 661, 852
MseI TTAA 2 cut(s) 44, 663
MslI CAYNNNNRTG 3 cut(s) 102, 148, 821
MspA1I CMGCKG 2 cut(s) 766, 955
MspI CCGG 1 cut(s) 868
MspR9I CCNGG 3 cut(s) 476, 598, 868
Mva1269I GAATGC 1 cut(s) 262
MvaI CCWGG 2 cut(s) 476, 598
MvnI CGCG 2 cut(s) 388, 766
MwoI GCNNNNNNNGC 8 cut(s) 62, 266, 290, 374, 450, 599, 856, 919
NciI CCSGG 1 cut(s) 868
NdeI CATATG 1 cut(s) 822
NdeII GATC 3 cut(s) 210, 434, 1005
NlaIII CATG 9 cut(s) 101, 202, 217, 512, 692, 753, 866, 941, 986
NlaIV GGNNCC 1 cut(s) 183
NmuCI GTSAC 2 cut(s) 558, 609
NspI RCATGY 1 cut(s) 512
NspV TTCGAA 1 cut(s) 74
PagI TCATGA 3 cut(s) 198, 688, 937
PceI AGGCCT 1 cut(s) 521
PctI GAATGC 1 cut(s) 262
PfeI GAWTC 1 cut(s) 703
PkrI GCNGC 7 cut(s) 64, 241, 262, 292, 376, 450, 768
PleI GAGTC 1 cut(s) 730
PpsI GAGTC 1 cut(s) 730
Psp124BI GAGCTC 1 cut(s) 427
Psp6I CCWGG 2 cut(s) 474, 596
PspGI CCWGG 2 cut(s) 474, 596
PspN4I GGNNCC 1 cut(s) 183
PspPI GGNCC 1 cut(s) 975
PsuI RGATCY 1 cut(s) 434
RseI CAYNNNNRTG 3 cut(s) 102, 148, 821
SacI GAGCTC 1 cut(s) 427
SacII CCGCGG 1 cut(s) 767
SaqAI TTAA 2 cut(s) 44, 663
SatI GCNGC 7 cut(s) 63, 240, 261, 291, 375, 449, 767
Sau3AI GATC 3 cut(s) 210, 434, 1005
Sau96I GGNCC 1 cut(s) 975
SchI GAGTC 1 cut(s) 731
ScrFI CCNGG 3 cut(s) 476, 598, 868
SduI GDGCHC 2 cut(s) 349, 427
SetI ASST 7 cut(s) 41, 334, 427, 433, 553, 565, 844
SfaNI GCATC 4 cut(s) 152, 226, 602, 837
Sfr303I CCGCGG 1 cut(s) 767
SfuI TTCGAA 1 cut(s) 74
SgrBI CCGCGG 1 cut(s) 767
SmiMI CAYNNNNRTG 3 cut(s) 102, 148, 821
Sse9I AATT 2 cut(s) 47, 759
SseBI AGGCCT 1 cut(s) 521
SsiI CCGC 7 cut(s) 388, 764, 766, 792, 880, 891, 953
SspI AATATT 1 cut(s) 70
SspMI CTAG 3 cut(s) 329, 906, 923
SstI GAGCTC 1 cut(s) 427
StuI AGGCCT 1 cut(s) 521
StyD4I CCNGG 3 cut(s) 474, 596, 866
StyI CCWWGG 1 cut(s) 285
TaaI ACNGT 1 cut(s) 11
TaiI ACGT 2 cut(s) 433, 565
TaqI TCGA 3 cut(s) 74, 247, 717
TasI AATT 2 cut(s) 47, 759
TauI GCSGC 1 cut(s) 769
TfiI GAWTC 1 cut(s) 703
Tru1I TTAA 2 cut(s) 44, 663
Tru9I TTAA 2 cut(s) 44, 663
TscAI CASTG 3 cut(s) 741, 760, 776
TseFI GTSAC 2 cut(s) 558, 609
TseI GCWGC 6 cut(s) 62, 239, 260, 290, 374, 448
Tsp45I GTSAC 2 cut(s) 558, 609
TspDTI ATGAA 6 cut(s) 114, 215, 294, 371, 476, 738
TspGWI ACGGA 3 cut(s) 268, 299, 628
TspRI CASTG 3 cut(s) 741, 760, 776
XapI RAATTY 2 cut(s) 47, 759
XceI RCATGY 1 cut(s) 512
XmiI GTMKAC 2 cut(s) 6, 725
XspI CTAG 3 cut(s) 329, 906, 923
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.