pycom10g10710

CDP-diacylglycerol-inositol 3-phosphatidyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0005659
Physical Location & Seq
Forward (+)
14178220 .. 14181072
2853 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 690 bp
ATGGCTAAGAAATCTGTAGCTCAACGAAGGCTAACGAAACTCGATGTCTATCTTTACATACCCAACATTATTGGGTACATAAGGGTTCTTATGAACTGTTTTGCCTTTGCCCAATGCTTCTCCAACAAGAAGCTTTTCTCTGTTCTCTATTTCGTCAGCTTTGTCTGCGATGGGGTAGATGGTTGGTGTGCTCGCAAATTCAACCAAGTTTCTACCTTTGGAGCTGTTTTGGACATGGTAACAGACAGGATAAGCACTGCGTGTCTCTTGGTAGTACTTTCACAAGTTTACCGGCCTGGCTTGGTTTTCCTCTCATTACTTGCCTTAGATATTGGTAGCCACTGGCTGCAGATGTACAGTACTTTTCTGTTAGGCAAGGCTAGTCACAAAGATGTGAAAGACAGCACTAATTGGCTTTTTAAAGCATACTACGGAAATCGGATGTTTATGGCTTACTGTTGTGTGGCATGTGAGGTCCTTTATATAATTCTATTTCTTCTTGCAAAGAACGAAACTGAGAACTTGATTGATGTTTTAACGGGGCTTGCAAAGCATATTACACCACTTTCTGTTCTAGTTGGTTTGAGTTTATTTGGATGGGCAGTCAAACAAGCAGTGAATCTTATACAGTTGAAGACAGCAGCAGATGTGTGTGTGCTTCATGATATCAACAAAAAGCAAAGCCCCTAA

Protein Analysis

230

Amino Acids

25.77

Weight (kDa)

9.11

Isoelectric Point (pI)

30.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CDP-OH_P_transf PF01066 19 - 116 4.8e-16 CDP-alcohol phosphatidyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcsI RAATTY 1 cut(s) 197
AdeI CACNNNGTG 1 cut(s) 261
AfaI GTAC 4 cut(s) 77, 276, 356, 361
AgsI TTSAA 2 cut(s) 202, 634
AjnI CCWGG 1 cut(s) 295
AluBI AGCT 4 cut(s) 20, 133, 159, 224
AluI AGCT 4 cut(s) 20, 133, 159, 224
Alw21I GWGCWC 1 cut(s) 193
Alw26I GTCTC 1 cut(s) 269
AoxI GGCC 1 cut(s) 293
ApeKI GCWGC 2 cut(s) 346, 641
ApoI RAATTY 1 cut(s) 197
Asp700I GAANNNNTTC 1 cut(s) 134
AspS9I GGNCC 1 cut(s) 475
AvaII GGWCC 1 cut(s) 475
BbsI GAAGAC 1 cut(s) 641
Bbv12I GWGCWC 1 cut(s) 193
BbvI GCAGC 2 cut(s) 333, 653
BccI CCATC 3 cut(s) 164, 173, 591
BciT130I CCWGG 1 cut(s) 297
BcoDI GTCTC 1 cut(s) 269
BfaI CTAG 2 cut(s) 381, 575
BfmI CTRYAG 2 cut(s) 15, 347
BisI GCNGC 2 cut(s) 347, 642
BlsI GCNGC 2 cut(s) 348, 643
BmcAI AGTACT 2 cut(s) 276, 361
Bme1390I CCNGG 1 cut(s) 297
Bme18I GGWCC 1 cut(s) 475
BmgT120I GGNCC 1 cut(s) 475
BmrFI CCNGG 1 cut(s) 297
BpiI GAAGAC 1 cut(s) 641
BsaBI GATNNNNATC 1 cut(s) 48
Bse118I RCCGGY 1 cut(s) 291
Bse1I ACTGG 1 cut(s) 347
Bse8I GATNNNNATC 1 cut(s) 48
BseBI CCWGG 1 cut(s) 297
BseGI GGATG 2 cut(s) 447, 602
BseJI GATNNNNATC 1 cut(s) 48
BseMII CTCAG 1 cut(s) 507
BseNI ACTGG 1 cut(s) 347
BseXI GCAGC 2 cut(s) 333, 653
BshFI GGCC 1 cut(s) 295
BsiHKAI GWGCWC 1 cut(s) 193
BsiSI CCGG 1 cut(s) 292
BsmAI GTCTC 1 cut(s) 269
BsnI GGCC 1 cut(s) 295
Bsp1286I GDGCHC 1 cut(s) 193
Bsp1407I TGTACA 1 cut(s) 354
BspANI GGCC 1 cut(s) 295
BspCNI CTCAG 1 cut(s) 508
BspHI TCATGA 1 cut(s) 661
BspMAI CTGCAG 1 cut(s) 351
BsrFI RCCGGY 1 cut(s) 291
BsrGI TGTACA 1 cut(s) 354
BsrI ACTGG 1 cut(s) 347
BssAI RCCGGY 1 cut(s) 291
Bst2UI CCWGG 1 cut(s) 297
Bst4CI ACNGT 4 cut(s) 98, 359, 458, 630
BstAUI TGTACA 1 cut(s) 354
BstC8I GCNNGC 2 cut(s) 193, 546
BstDEI CTNAG 3 cut(s) 6, 325, 516
BstF5I GGATG 2 cut(s) 447, 602
BstMAI GTCTC 1 cut(s) 269
BstMWI GCNNNNNNNGC 2 cut(s) 165, 550
BstNI CCWGG 1 cut(s) 297
BstNSI RCATGY 1 cut(s) 471
BstSCI CCNGG 1 cut(s) 295
BstSFI CTRYAG 2 cut(s) 15, 347
BstV1I GCAGC 2 cut(s) 333, 653
BstV2I GAAGAC 1 cut(s) 641
BsuRI GGCC 1 cut(s) 295
BtgZI GCGATG 1 cut(s) 183
BtsCI GGATG 2 cut(s) 447, 602
BtsI GCAGTG 2 cut(s) 255, 621
BtsIMutI CAGTG 3 cut(s) 255, 340, 621
Cac8I GCNNGC 2 cut(s) 193, 546
CciI TCATGA 1 cut(s) 661
Cfr10I RCCGGY 1 cut(s) 291
Cfr13I GGNCC 1 cut(s) 475
Csp6I GTAC 4 cut(s) 76, 275, 355, 360
CviAII CATG 3 cut(s) 235, 468, 662
CviQI GTAC 4 cut(s) 76, 275, 355, 360
DdeI CTNAG 3 cut(s) 6, 325, 516
DraI TTTAAA 1 cut(s) 421
DraIII CACNNNGTG 1 cut(s) 261
Eco32I GATATC 1 cut(s) 667
Eco47I GGWCC 1 cut(s) 475
EcoO109I RGGNCCY 1 cut(s) 475
EcoRII CCWGG 1 cut(s) 295
EcoRV GATATC 1 cut(s) 667
FaeI CATG 3 cut(s) 238, 471, 665
FatI CATG 3 cut(s) 234, 467, 661
Fnu4HI GCNGC 2 cut(s) 347, 642
FokI GGATG 2 cut(s) 454, 609
Fsp4HI GCNGC 2 cut(s) 347, 642
FspBI CTAG 2 cut(s) 381, 575
GluI GCNGC 2 cut(s) 347, 642
HaeIII GGCC 1 cut(s) 295
HapII CCGG 1 cut(s) 292
Hin1II CATG 3 cut(s) 238, 471, 665
HindIII AAGCTT 1 cut(s) 131
HinfI GANTC 1 cut(s) 619
HpaII CCGG 1 cut(s) 292
Hpy166II GTNNAC 1 cut(s) 289
Hpy188I TCNGA 1 cut(s) 441
Hpy188III TCNNGA 1 cut(s) 662
Hpy8I GTNNAC 1 cut(s) 289
HpyAV CCTTC 1 cut(s) 21
HpyCH4III ACNGT 4 cut(s) 98, 359, 458, 630
HpyCH4V TGCA 3 cut(s) 349, 503, 548
HpyF10VI GCNNNNNNNGC 2 cut(s) 165, 550
HpyF3I CTNAG 3 cut(s) 6, 325, 516
Hsp92II CATG 3 cut(s) 238, 471, 665
LmnI GCTCC 1 cut(s) 221
LpnPI CCDG 5 cut(s) 232, 282, 305, 309, 328
Lsp1109I GCAGC 2 cut(s) 333, 653
MaeI CTAG 2 cut(s) 381, 575
MaeIII GTNAC 2 cut(s) 238, 383
MboII GAAGA 2 cut(s) 488, 646
MhlI GDGCHC 1 cut(s) 193
MluCI AATT 3 cut(s) 197, 409, 486
MmeI TCCRAC 1 cut(s) 147
MnlI CCTC 2 cut(s) 320, 466
MroXI GAANNNNTTC 1 cut(s) 134
MseI TTAA 2 cut(s) 420, 536
MslI CAYNNNNRTG 1 cut(s) 390
MspI CCGG 1 cut(s) 292
MspR9I CCNGG 1 cut(s) 297
MvaI CCWGG 1 cut(s) 297
MwoI GCNNNNNNNGC 2 cut(s) 165, 550
NlaIII CATG 3 cut(s) 238, 471, 665
NmuCI GTSAC 1 cut(s) 383
NspI RCATGY 1 cut(s) 471
PagI TCATGA 1 cut(s) 661
PdmI GAANNNNTTC 1 cut(s) 134
PfeI GAWTC 1 cut(s) 619
PkrI GCNGC 2 cut(s) 348, 643
PpuMI RGGWCCY 1 cut(s) 475
Psp5II RGGWCCY 1 cut(s) 475
Psp6I CCWGG 1 cut(s) 295
PspGI CCWGG 1 cut(s) 295
PspPI GGNCC 1 cut(s) 475
PspPPI RGGWCCY 1 cut(s) 475
PstI CTGCAG 1 cut(s) 351
RsaI GTAC 4 cut(s) 77, 276, 356, 361
RsaNI GTAC 4 cut(s) 76, 275, 355, 360
RseI CAYNNNNRTG 1 cut(s) 390
SaqAI TTAA 2 cut(s) 420, 536
SatI GCNGC 2 cut(s) 347, 642
Sau96I GGNCC 1 cut(s) 475
ScaI AGTACT 2 cut(s) 276, 361
ScrFI CCNGG 1 cut(s) 297
SduI GDGCHC 1 cut(s) 193
SetI ASST 6 cut(s) 22, 135, 161, 218, 226, 477
SfcI CTRYAG 2 cut(s) 15, 347
SinI GGWCC 1 cut(s) 475
SmiMI CAYNNNNRTG 1 cut(s) 390
Sse9I AATT 3 cut(s) 197, 409, 486
SspMI CTAG 2 cut(s) 381, 575
StyD4I CCNGG 1 cut(s) 295
TaaI ACNGT 4 cut(s) 98, 359, 458, 630
TaqI TCGA 1 cut(s) 42
TasI AATT 3 cut(s) 197, 409, 486
TatI WGTACW 3 cut(s) 274, 354, 359
TfiI GAWTC 1 cut(s) 619
Tru1I TTAA 2 cut(s) 420, 536
Tru9I TTAA 2 cut(s) 420, 536
TscAI CASTG 3 cut(s) 262, 347, 621
TseFI GTSAC 1 cut(s) 383
TseI GCWGC 2 cut(s) 346, 641
Tsp45I GTSAC 1 cut(s) 383
TspDTI ATGAA 2 cut(s) 107, 650
TspGWI ACGGA 1 cut(s) 447
TspRI CASTG 3 cut(s) 262, 347, 621
VpaK11BI GGWCC 1 cut(s) 475
XapI RAATTY 1 cut(s) 197
XceI RCATGY 1 cut(s) 471
XmnI GAANNNNTTC 1 cut(s) 134
XspI CTAG 2 cut(s) 381, 575
ZrmI AGTACT 2 cut(s) 276, 361
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.