pycom10g12250

At2g17340-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0011219
Physical Location & Seq
Reverse (-)
15801766 .. 15804324
2559 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g12250.3

Sequence Viewer

Length: 1107 bp
ATGGAGAGCGCATCAGAAATGGTGCCATTTCCACTGCTGATGACGCCGATCGAATCCAACTACAGAGCCTGCACCATCCCCTACAGATTCCCCTCCGACAACCCCCGCAAGCCCACGCCGACTGAGCTCTCCTGGATCGACCTCTTCTACAACTCCACCCCCTCCTTCAAGAAACGAGCTGAGTCCGACGCTTCGGTCCCTGATGCTACGGCCAGAGCTGAGAAATTTGCTCAAAGGTACACTGAAATACTTGAGGACTTGAAGAAGGATCCTGAAAGTCACGGTGGCCCGCCGGATTGCATTCTTCTTTGCAGACTTCGTGAGCAAGTTCTTAGGGAATTGGGATTCCAAGACATTTTCAAGAAAGTCAAGGATGAAGAGAATGCCAAGGCCATATCCCTGTTTGAGAATGTAGTGGATCTTAATGATGCTATTGAAGATGAAGTCAAGCGGGTAGAGAATCTGGTTAGAGGAATATTTGCCGGAAACATATTCGATCTTGGTTCTGCACAGCTTGCAGAAGTTTTCTCAAAGGATGGGATGTCCTTTTTAGCTAGTTGTCAAAACCTCGTCCCTCGACCTTGGGTTATCGATGACTTGGACGCCTTCAAATTAAAATGGACCAAGAAATCTTGGAAAAAGGCTGTTATATTTGTCGACAATTCTGGTGCTGATATTATTTTGGGTATTTTACCATTTGCAAGAGAATTACTCCGGCGTGGGACACAGGTTGTGTTGGCAGCTAATGACTTGCCTTCTATCAATGATGTAACTTACACTGAACTGATTGAGATTATATCAAAGTTGAAGGACAAGAACGGAACTCTCAAAGGTGTTGATACTTCAAAGCTTCTGATTGCTAATTCAGGCAATGATTTGCCAGTTATTGATCTCTCAAGAGTGCCACAAGAGCTAGCCTACTTGGCAAGTGATGCAGACCTCGTTATTTTGGAAGGAATGGGACGTGGGATAGAGACAAATCTTTATGCGCAATTTAAGTGTGATTCCCTAAAGATTGGGATGGTGAAACATTCAGAGGTCGCCCAGTTTCTAGGAGGAAGGCTTTATGATTGCGTATTCAAATACAACGAAGTTTTGTTGAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00770 ko01100 map00770 map01100
Pfam Domains
Protein Families
DUF89

Protein Analysis

369

Amino Acids

41.21

Weight (kDa)

5.03

Isoelectric Point (pI)

35.52

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011219)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G17320 AT2G17320 AT2G17340 AT4G35360 AT4G35360
fragaria_vesca FvH4_2g16941 FvH4_2g16941
malus_domestica MD05G1141200.v1.1 MD10G1142600.v1.1
prunus_persica Prupe.8G181200_v2.0.a1
pyrus_communis pycom10g12250
rosa_chinensis RchiOBHm_Chr6g0281141
rosa_laevigata RLG00000012991
rosa_multiflora Rmu_sc0001705.1_g000019
rosa_roxburghii Rroxscaffold_7G00187260
rosa_rugosa Rorug06G0139200
rosa_samantha Rh6BG254200 Rh6CG253600 Rh6DG245600
rosa_wichuraiana Rw6G021790

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 194
Acc16I TGCGCA 1 cut(s) 990
AccB1I GGYRCC 1 cut(s) 22
AccI GTMKAC 1 cut(s) 657
AciI CCGC 3 cut(s) 106, 290, 451
AclWI GGATC 4 cut(s) 143, 263, 276, 426
AcoI YGGCCR 1 cut(s) 210
AcsI RAATTY 1 cut(s) 224
AcyI GRCGYC 2 cut(s) 44, 603
AfaI GTAC 1 cut(s) 239
AgsI TTSAA 8 cut(s) 169, 262, 361, 437, 610, 808, 846, 1081
AjiI CACGTC 1 cut(s) 965
AjnI CCWGG 1 cut(s) 131
AluBI AGCT 8 cut(s) 127, 179, 218, 514, 554, 743, 850, 913
AluI AGCT 8 cut(s) 127, 179, 218, 514, 554, 743, 850, 913
Alw21I GWGCWC 1 cut(s) 129
Alw26I GTCTC 1 cut(s) 968
AlwI GGATC 4 cut(s) 143, 263, 276, 426
AlwNI CAGNNNCTG 1 cut(s) 69
AoxI GGCC 3 cut(s) 210, 286, 390
ApeKI GCWGC 1 cut(s) 740
ApoI RAATTY 1 cut(s) 224
AspLEI GCGC 2 cut(s) 11, 991
AspS9I GGNCC 3 cut(s) 196, 287, 621
AsuHPI GGTGA 1 cut(s) 1036
AsuNHI GCTAGC 1 cut(s) 913
AvaII GGWCC 2 cut(s) 196, 621
BamHI GGATCC 1 cut(s) 268
BanI GGYRCC 1 cut(s) 22
BanII GRGCYC 1 cut(s) 129
Bbv12I GWGCWC 1 cut(s) 129
BbvI GCAGC 1 cut(s) 752
BccI CCATC 3 cut(s) 83, 530, 1015
BceAI ACGGC 1 cut(s) 225
BciT130I CCWGG 1 cut(s) 133
BcoDI GTCTC 1 cut(s) 968
BfaI CTAG 3 cut(s) 555, 914, 1052
BfmI CTRYAG 2 cut(s) 61, 82
BglI GCCNNNNNGGC 1 cut(s) 923
BisI GCNGC 1 cut(s) 741
BlsI GCNGC 1 cut(s) 742
Bme1390I CCNGG 1 cut(s) 133
Bme18I GGWCC 2 cut(s) 196, 621
BmgBI CACGTC 1 cut(s) 965
BmgT120I GGNCC 3 cut(s) 196, 287, 621
BmiI GGNNCC 3 cut(s) 24, 198, 270
BmrFI CCNGG 1 cut(s) 133
BmrI ACTGGG 1 cut(s) 1039
BmsI GCATC 4 cut(s) 20, 193, 418, 922
BmtI GCTAGC 1 cut(s) 917
BmuI ACTGGG 1 cut(s) 1039
BplI GAGNNNNNCTC 2 cut(s) 696, 728
BpuEI CTTGAG 2 cut(s) 272, 880
Bsa29I ATCGAT 1 cut(s) 591
BsaHI GRCGYC 2 cut(s) 44, 603
BsaJI CCNNGG 2 cut(s) 387, 581
Bse1I ACTGG 2 cut(s) 881, 1045
Bse3DI GCAATG 1 cut(s) 877
BseBI CCWGG 1 cut(s) 133
BseCI ATCGAT 1 cut(s) 591
BseDI CCNNGG 2 cut(s) 387, 581
BseGI GGATG 5 cut(s) 75, 379, 541, 546, 1026
BseMI GCAATG 1 cut(s) 877
BseMII CTCAG 3 cut(s) 114, 171, 210
BseNI ACTGG 2 cut(s) 881, 1045
BseXI GCAGC 1 cut(s) 752
BsgI GTGCAG 2 cut(s) 55, 492
Bsh1285I CGRYCG 1 cut(s) 51
BshFI GGCC 3 cut(s) 212, 288, 392
BshNI GGYRCC 1 cut(s) 22
BshVI ATCGAT 1 cut(s) 591
BsiEI CGRYCG 1 cut(s) 51
BsiHKAI GWGCWC 1 cut(s) 129
BsiSI CCGG 3 cut(s) 293, 483, 715
BslFI GGGAC 4 cut(s) 182, 557, 736, 975
BsmAI GTCTC 1 cut(s) 968
BsmFI GGGAC 4 cut(s) 182, 557, 736, 975
BsmI GAATGC 2 cut(s) 300, 388
BsnI GGCC 3 cut(s) 212, 288, 392
Bsp1286I GDGCHC 1 cut(s) 129
Bsp143I GATC 6 cut(s) 48, 135, 268, 418, 496, 889
BspACI CCGC 3 cut(s) 106, 290, 451
BspANI GGCC 3 cut(s) 212, 288, 392
BspCNI CTCAG 3 cut(s) 115, 172, 211
BspDI ATCGAT 1 cut(s) 591
BspLI GGNNCC 3 cut(s) 24, 198, 270
BspOI GCTAGC 1 cut(s) 917
BspPI GGATC 4 cut(s) 143, 263, 276, 426
BspT107I GGYRCC 1 cut(s) 22
BsrDI GCAATG 1 cut(s) 877
BsrI ACTGG 2 cut(s) 881, 1045
BssECI CCNNGG 2 cut(s) 387, 581
BssMI GATC 6 cut(s) 48, 135, 268, 418, 496, 889
BssNI GRCGYC 2 cut(s) 44, 603
BssT1I CCWWGG 2 cut(s) 387, 581
Bst2UI CCWGG 1 cut(s) 133
Bst4CI ACNGT 1 cut(s) 284
Bst6I CTCTTC 2 cut(s) 149, 372
BstACI GRCGYC 2 cut(s) 44, 603
BstAPI GCANNNNNTGC 2 cut(s) 515, 932
BstC8I GCNNGC 5 cut(s) 70, 110, 290, 516, 915
BstDEI CTNAG 4 cut(s) 123, 180, 219, 332
BstF5I GGATG 5 cut(s) 75, 379, 541, 546, 1026
BstHHI GCGC 2 cut(s) 11, 991
BstKTI GATC 6 cut(s) 51, 138, 271, 421, 499, 892
BstMAI GTCTC 1 cut(s) 968
BstMBI GATC 6 cut(s) 48, 135, 268, 418, 496, 889
BstMCI CGRYCG 1 cut(s) 51
BstMWI GCNNNNNNNGC 6 cut(s) 43, 124, 515, 910, 923, 932
BstNI CCWGG 1 cut(s) 133
BstSCI CCNGG 1 cut(s) 131
BstSFI CTRYAG 2 cut(s) 61, 82
BstV1I GCAGC 1 cut(s) 752
BstX2I RGATCY 2 cut(s) 268, 418
BstYI RGATCY 2 cut(s) 268, 418
Bsu15I ATCGAT 1 cut(s) 591
BsuRI GGCC 3 cut(s) 212, 288, 392
BsuTUI ATCGAT 1 cut(s) 591
BtrI CACGTC 1 cut(s) 965
BtsCI GGATG 5 cut(s) 75, 379, 541, 546, 1026
BtsI GCAGTG 1 cut(s) 32
BtsIMutI CAGTG 3 cut(s) 32, 240, 777
Cac8I GCNNGC 5 cut(s) 70, 110, 290, 516, 915
CaiI CAGNNNCTG 1 cut(s) 69
CfoI GCGC 2 cut(s) 11, 991
Cfr13I GGNCC 3 cut(s) 196, 287, 621
ClaI ATCGAT 1 cut(s) 591
CseI GACGC 3 cut(s) 52, 197, 611
Csp6I GTAC 1 cut(s) 238
CviQI GTAC 1 cut(s) 238
DdeI CTNAG 4 cut(s) 123, 180, 219, 332
DpnI GATC 6 cut(s) 50, 137, 270, 420, 498, 891
DpnII GATC 6 cut(s) 48, 135, 268, 418, 496, 889
DrdI GACNNNNNNGTC 1 cut(s) 194
DseDI GACNNNNNNGTC 1 cut(s) 194
EaeI YGGCCR 1 cut(s) 210
Eam1104I CTCTTC 2 cut(s) 149, 372
EarI CTCTTC 2 cut(s) 149, 372
Ecl136II GAGCTC 1 cut(s) 127
Eco130I CCWWGG 2 cut(s) 387, 581
Eco24I GRGCYC 1 cut(s) 129
Eco47I GGWCC 2 cut(s) 196, 621
Eco53kI GAGCTC 1 cut(s) 127
EcoICRI GAGCTC 1 cut(s) 127
EcoRII CCWGG 1 cut(s) 131
EcoT14I CCWWGG 2 cut(s) 387, 581
EcoT38I GRGCYC 1 cut(s) 129
ErhI CCWWGG 2 cut(s) 387, 581
FaiI YATR 6 cut(s) 395, 491, 650, 797, 987, 1068
FaqI GGGAC 4 cut(s) 182, 557, 736, 975
FauI CCCGC 3 cut(s) 113, 297, 444
FblI GTMKAC 1 cut(s) 657
Fnu4HI GCNGC 1 cut(s) 741
FokI GGATG 5 cut(s) 62, 386, 548, 553, 1033
FriOI GRGCYC 1 cut(s) 129
Fsp4HI GCNGC 1 cut(s) 741
FspBI CTAG 3 cut(s) 555, 914, 1052
FspI TGCGCA 1 cut(s) 990
GlaI GCGC 2 cut(s) 10, 990
GluI GCNGC 1 cut(s) 741
HaeIII GGCC 3 cut(s) 212, 288, 392
HapII CCGG 3 cut(s) 293, 483, 715
HgaI GACGC 3 cut(s) 52, 197, 611
HhaI GCGC 2 cut(s) 11, 991
Hin1I GRCGYC 2 cut(s) 44, 603
Hin6I GCGC 2 cut(s) 9, 989
HinP1I GCGC 2 cut(s) 9, 989
HincII GTYRAC 1 cut(s) 658
HindII GTYRAC 1 cut(s) 658
HindIII AAGCTT 1 cut(s) 848
HinfI GANTC 6 cut(s) 53, 87, 182, 345, 460, 1004
HpaII CCGG 3 cut(s) 293, 483, 715
HphI GGTGA 1 cut(s) 1036
Hpy166II GTNNAC 2 cut(s) 240, 658
Hpy188I TCNGA 5 cut(s) 16, 97, 187, 855, 1036
Hpy188III TCNNGA 5 cut(s) 169, 272, 320, 361, 897
Hpy8I GTNNAC 2 cut(s) 240, 658
Hpy99I CGWCG 1 cut(s) 191
HpyAV CCTTC 7 cut(s) 175, 259, 616, 765, 802, 947, 1053
HpyCH4III ACNGT 1 cut(s) 284
HpyCH4IV ACGT 1 cut(s) 964
HpyCH4V TGCA 7 cut(s) 72, 300, 312, 509, 518, 701, 935
HpyF10VI GCNNNNNNNGC 6 cut(s) 43, 124, 515, 910, 923, 932
HpyF3I CTNAG 4 cut(s) 123, 180, 219, 332
HpySE526I ACGT 1 cut(s) 964
Hsp92I GRCGYC 2 cut(s) 44, 603
HspAI GCGC 2 cut(s) 9, 989
Kzo9I GATC 6 cut(s) 48, 135, 268, 418, 496, 889
Lsp1109I GCAGC 1 cut(s) 752
LweI GCATC 4 cut(s) 20, 193, 418, 922
MaeI CTAG 3 cut(s) 555, 914, 1052
MaeII ACGT 1 cut(s) 964
MaeIII GTNAC 2 cut(s) 278, 769
MalI GATC 6 cut(s) 50, 137, 270, 420, 498, 891
MboI GATC 6 cut(s) 48, 135, 268, 418, 496, 889
MboII GAAGA 5 cut(s) 136, 274, 296, 389, 449
MflI RGATCY 2 cut(s) 268, 418
MhlI GDGCHC 1 cut(s) 129
MluCI AATT 7 cut(s) 224, 338, 611, 661, 707, 862, 992
MlyI GAGTC 1 cut(s) 191
MmeI TCCRAC 3 cut(s) 81, 120, 210
MseI TTAA 3 cut(s) 423, 614, 996
MspI CCGG 3 cut(s) 293, 483, 715
MspR9I CCNGG 1 cut(s) 133
Mva1269I GAATGC 2 cut(s) 300, 388
MvaI CCWGG 1 cut(s) 133
MwoI GCNNNNNNNGC 6 cut(s) 43, 124, 515, 910, 923, 932
NdeII GATC 6 cut(s) 48, 135, 268, 418, 496, 889
NheI GCTAGC 1 cut(s) 913
NlaIV GGNNCC 3 cut(s) 24, 198, 270
NmuCI GTSAC 1 cut(s) 278
NsbI TGCGCA 1 cut(s) 990
PctI GAATGC 2 cut(s) 300, 388
PfeI GAWTC 5 cut(s) 53, 87, 345, 460, 1004
PfoI TCCNGGA 1 cut(s) 131
PkrI GCNGC 1 cut(s) 742
Ple19I CGATCG 1 cut(s) 51
PleI GAGTC 1 cut(s) 190
PpsI GAGTC 1 cut(s) 190
Psp124BI GAGCTC 1 cut(s) 129
Psp6I CCWGG 1 cut(s) 131
PspGI CCWGG 1 cut(s) 131
PspN4I GGNNCC 3 cut(s) 24, 198, 270
PspPI GGNCC 3 cut(s) 196, 287, 621
PstNI CAGNNNCTG 1 cut(s) 69
PsuI RGATCY 2 cut(s) 268, 418
PvuI CGATCG 1 cut(s) 51
RsaI GTAC 1 cut(s) 239
RsaNI GTAC 1 cut(s) 238
SacI GAGCTC 1 cut(s) 129
SalI GTCGAC 1 cut(s) 656
SaqAI TTAA 3 cut(s) 423, 614, 996
SatI GCNGC 1 cut(s) 741
Sau3AI GATC 6 cut(s) 48, 135, 268, 418, 496, 889
Sau96I GGNCC 3 cut(s) 196, 287, 621
SchI GAGTC 1 cut(s) 191
ScrFI CCNGG 1 cut(s) 133
SduI GDGCHC 1 cut(s) 129
SfaNI GCATC 4 cut(s) 20, 193, 418, 922
SfcI CTRYAG 2 cut(s) 61, 82
SinI GGWCC 2 cut(s) 196, 621
SmlI CTYRAG 2 cut(s) 251, 895
SmoI CTYRAG 2 cut(s) 251, 895
Sse9I AATT 7 cut(s) 224, 338, 611, 661, 707, 862, 992
SsiI CCGC 3 cut(s) 106, 290, 451
SspI AATATT 1 cut(s) 477
SspMI CTAG 3 cut(s) 555, 914, 1052
SstI GAGCTC 1 cut(s) 129
StyD4I CCNGG 1 cut(s) 131
StyI CCWWGG 2 cut(s) 387, 581
TaaI ACNGT 1 cut(s) 284
TaiI ACGT 1 cut(s) 967
TaqI TCGA 6 cut(s) 51, 138, 495, 577, 591, 657
TaqII GACCGA 1 cut(s) 184
TasI AATT 7 cut(s) 224, 338, 611, 661, 707, 862, 992
TfiI GAWTC 5 cut(s) 53, 87, 345, 460, 1004
Tru1I TTAA 3 cut(s) 423, 614, 996
Tru9I TTAA 3 cut(s) 423, 614, 996
TscAI CASTG 3 cut(s) 39, 247, 784
TseFI GTSAC 1 cut(s) 278
TseI GCWGC 1 cut(s) 740
Tsp45I GTSAC 1 cut(s) 278
TspDTI ATGAA 2 cut(s) 390, 456
TspGWI ACGGA 1 cut(s) 834
TspRI CASTG 3 cut(s) 39, 247, 784
VpaK11BI GGWCC 2 cut(s) 196, 621
XapI RAATTY 1 cut(s) 224
XmiI GTMKAC 1 cut(s) 657
XspI CTAG 3 cut(s) 555, 914, 1052
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.