pycom10g12300

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0012981
Physical Location & Seq
Reverse (-)
15830451 .. 15831897
1447 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g12300.1

Sequence Viewer

Length: 753 bp
ATGGGACCTTCTTCCACAACTCAATCCCTCACTCACTTCACCTCCCTCCCCAAAACCCTAATCCTCTCCCCAGAGTCCCACTCTTCATCCCTGCAACTCTCAAATCCCAAGAGACTGAAGGCCACCATTACAATGAGAGACAGAAGCAACAACCCTCGGCCGTTGCAGAAGGGGAGGTTCCTCAGCATCGAAGCCATCCAGACCGTGCAGGCGTTGAAGAGGGCGCAGAAAGATCAGAGCATTTTGAGCCAAGTTTTCGATTCCAAGTTCCGGCGGTTGTTGAAGCTCGATATGATGGCTGTCCTCCGCGACCTCCTCCGCCAGAACGAGTGCATCTTGGCCCTCAAGGTCTTTGAAGACATTCGAAAGGAACATTGGTACAGGCCACAGGTTTCATTGTATTCCGATATGATTAGAGTAATGGCTAGCAATGGATTGTTTGAACAAGTTGAACTTCTTTGCTTGTGCTTGAAAAAGGAAACTAATTTACAGTCTGAAATCGAGGCATTTAATGCTCTAATGACAACATTGATAAGTTTTAACCTCCCTAAACTTGCAATCGAGTGTTATTACTTGATGAAAGAGGTGGGGTGTGAACCTGATAGGTCATCCTTTAGAATACTTGTTAATGGTCTAGAGTCGATGGGAGAGACAGGTCGTTCGGGTATTGTAAGGCAGGATGCTCAAAAGATTTACAGGGAATCTCTGGAGTTTCTAGAGGAGAATGAAGAAATGGAAGTGAGCCAGCAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR_2 PPR_3

Protein Analysis

251

Amino Acids

28.76

Weight (kDa)

8.24

Isoelectric Point (pI)

58.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 169 - 212 8.8e-07 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012981)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G09315
fragaria_vesca FvH4_2g17040
malus_domestica MD10G1143100.v1.1
prunus_persica Prupe.8G182100_v2.0.a1 Prupe.8G182100_v2.0.a1
pyrus_communis pycom05g13780 pycom10g12300
rosa_chinensis RchiOBHm_Chr6g0281221
rosa_laevigata RLG00000012983
rosa_multiflora Rmu_sc0000847.1_g000051 Rmu_sc0027531.1_g000002
rosa_roxburghii Rroxscaffold_7G00187210
rosa_rugosa Rorug06G0139800
rosa_samantha Rh6AG251700 Rh6BG255000 Rh6CG254500 Rh6DG246300
rosa_wichuraiana Rw6G021860

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 309
AciI CCGC 3 cut(s) 274, 307, 319
AcoI YGGCCR 1 cut(s) 158
AcuI CTGAAG 1 cut(s) 137
AfaI GTAC 1 cut(s) 380
AfiI CCNNNNNNNGG 1 cut(s) 270
AgsI TTSAA 6 cut(s) 217, 283, 356, 443, 452, 472
AjuI GAANNNNNNNTTGG 2 cut(s) 358, 390
AluBI AGCT 1 cut(s) 286
AluI AGCT 1 cut(s) 286
Alw26I GTCTC 3 cut(s) 106, 132, 644
AoxI GGCC 4 cut(s) 120, 158, 339, 383
ArsI GACNNNNNNTTYG 2 cut(s) 643, 675
Asp700I GAANNNNTTC 1 cut(s) 360
AspLEI GCGC 1 cut(s) 226
AspS9I GGNCC 2 cut(s) 5, 340
AsuHPI GGTGA 1 cut(s) 31
AsuII TTCGAA 1 cut(s) 364
AsuNHI GCTAGC 1 cut(s) 425
AvaII GGWCC 1 cut(s) 5
BbsI GAAGAC 1 cut(s) 363
BbvCI CCTCAGC 1 cut(s) 182
BccI CCATC 3 cut(s) 203, 289, 637
BceAI ACGGC 1 cut(s) 145
BcoDI GTCTC 3 cut(s) 106, 132, 644
BfaI CTAG 3 cut(s) 426, 635, 716
Bme18I GGWCC 1 cut(s) 5
BmgT120I GGNCC 2 cut(s) 5, 340
BmiI GGNNCC 2 cut(s) 6, 179
BmsI GCATC 3 cut(s) 195, 342, 670
BmtI GCTAGC 1 cut(s) 429
BpiI GAAGAC 1 cut(s) 363
BplI GAGNNNNNCTC 2 cut(s) 65, 97
BpmI CTGGAG 1 cut(s) 728
Bpu10I CCTNAGC 1 cut(s) 182
Bpu14I TTCGAA 1 cut(s) 364
BpuEI CTTGAG 1 cut(s) 329
BsaJI CCNNGG 1 cut(s) 155
BsaXI ACNNNNNCTCC 2 cut(s) 26, 56
Bsc4I CCNNNNNNNGG 1 cut(s) 270
Bse3DI GCAATG 1 cut(s) 436
BseDI CCNNGG 1 cut(s) 155
BseGI GGATG 4 cut(s) 86, 195, 608, 685
BseLI CCNNNNNNNGG 1 cut(s) 270
BseMI GCAATG 1 cut(s) 436
BseMII CTCAG 1 cut(s) 196
BseRI GAGGAG 2 cut(s) 305, 734
BseX3I CGGCCG 1 cut(s) 158
BsgI GTGCAG 1 cut(s) 227
Bsh1236I CGCG 1 cut(s) 309
Bsh1285I CGRYCG 1 cut(s) 161
BshFI GGCC 4 cut(s) 122, 160, 341, 385
BsiEI CGRYCG 1 cut(s) 161
BsiSI CCGG 1 cut(s) 271
BslFI GGGAC 2 cut(s) 18, 61
BslI CCNNNNNNNGG 1 cut(s) 270
BsmAI GTCTC 3 cut(s) 106, 132, 644
BsmFI GGGAC 2 cut(s) 18, 61
BsnI GGCC 4 cut(s) 122, 160, 341, 385
Bsp119I TTCGAA 1 cut(s) 364
Bsp143I GATC 1 cut(s) 232
BspACI CCGC 3 cut(s) 274, 307, 319
BspANI GGCC 4 cut(s) 122, 160, 341, 385
BspCNI CTCAG 1 cut(s) 195
BspFNI CGCG 1 cut(s) 309
BspLI GGNNCC 2 cut(s) 6, 179
BspOI GCTAGC 1 cut(s) 429
BspT104I TTCGAA 1 cut(s) 364
BsrDI GCAATG 1 cut(s) 436
BssECI CCNNGG 1 cut(s) 155
BssMI GATC 1 cut(s) 232
Bst4CI ACNGT 2 cut(s) 205, 492
Bst6I CTCTTC 2 cut(s) 88, 212
BstAPI GCANNNNNTGC 1 cut(s) 512
BstBI TTCGAA 1 cut(s) 364
BstC8I GCNNGC 3 cut(s) 210, 427, 746
BstDEI CTNAG 1 cut(s) 182
BstF5I GGATG 4 cut(s) 86, 195, 608, 685
BstFNI CGCG 1 cut(s) 309
BstHHI GCGC 1 cut(s) 226
BstKTI GATC 1 cut(s) 235
BstMAI GTCTC 3 cut(s) 106, 132, 644
BstMBI GATC 1 cut(s) 232
BstMCI CGRYCG 1 cut(s) 161
BstMWI GCNNNNNNNGC 2 cut(s) 246, 512
BstUI CGCG 1 cut(s) 309
BstV2I GAAGAC 1 cut(s) 363
BstZI CGGCCG 1 cut(s) 158
BsuRI GGCC 4 cut(s) 122, 160, 341, 385
BtsCI GGATG 4 cut(s) 86, 195, 608, 685
Cac8I GCNNGC 3 cut(s) 210, 427, 746
CfoI GCGC 1 cut(s) 226
Cfr13I GGNCC 2 cut(s) 5, 340
Csp6I GTAC 1 cut(s) 379
CviQI GTAC 1 cut(s) 379
DdeI CTNAG 1 cut(s) 182
DpnI GATC 1 cut(s) 234
DpnII GATC 1 cut(s) 232
EaeI YGGCCR 1 cut(s) 158
EagI CGGCCG 1 cut(s) 158
Eam1104I CTCTTC 2 cut(s) 88, 212
EarI CTCTTC 2 cut(s) 88, 212
EciI GGCGGA 1 cut(s) 308
EclXI CGGCCG 1 cut(s) 158
Eco47I GGWCC 1 cut(s) 5
Eco52I CGGCCG 1 cut(s) 158
Eco57I CTGAAG 1 cut(s) 137
EcoO109I RGGNCCY 1 cut(s) 5
FaiI YATR 2 cut(s) 293, 410
FalI AAGNNNNNCTT 2 cut(s) 438, 470
FaqI GGGAC 2 cut(s) 18, 61
FokI GGATG 4 cut(s) 73, 182, 595, 692
FspBI CTAG 3 cut(s) 426, 635, 716
GlaI GCGC 1 cut(s) 225
GsuI CTGGAG 1 cut(s) 728
HaeIII GGCC 4 cut(s) 122, 160, 341, 385
HapII CCGG 1 cut(s) 271
HhaI GCGC 1 cut(s) 226
Hin6I GCGC 1 cut(s) 224
HinP1I GCGC 1 cut(s) 224
HinfI GANTC 4 cut(s) 74, 260, 638, 701
HpaII CCGG 1 cut(s) 271
HphI GGTGA 1 cut(s) 31
Hpy166II GTNNAC 1 cut(s) 596
Hpy188I TCNGA 3 cut(s) 237, 406, 496
Hpy188III TCNNGA 4 cut(s) 199, 635, 707, 716
Hpy8I GTNNAC 1 cut(s) 596
HpyAV CCTTC 3 cut(s) 18, 112, 163
HpyCH4III ACNGT 2 cut(s) 205, 492
HpyCH4V TGCA 5 cut(s) 94, 166, 208, 333, 557
HpyF10VI GCNNNNNNNGC 2 cut(s) 246, 512
HpyF3I CTNAG 1 cut(s) 182
HspAI GCGC 1 cut(s) 224
Kzo9I GATC 1 cut(s) 232
LweI GCATC 3 cut(s) 195, 342, 670
MaeI CTAG 3 cut(s) 426, 635, 716
MalI GATC 1 cut(s) 234
MboI GATC 1 cut(s) 232
MboII GAAGA 5 cut(s) 3, 75, 229, 368, 740
MluCI AATT 1 cut(s) 484
MlyI GAGTC 2 cut(s) 83, 647
MroXI GAANNNNTTC 1 cut(s) 360
MseI TTAA 3 cut(s) 510, 540, 627
MslI CAYNNNNRTG 1 cut(s) 131
MspI CCGG 1 cut(s) 271
MvnI CGCG 1 cut(s) 309
MwoI GCNNNNNNNGC 2 cut(s) 246, 512
NdeII GATC 1 cut(s) 232
NheI GCTAGC 1 cut(s) 425
NlaIV GGNNCC 2 cut(s) 6, 179
NmeAIII GCCGAG 1 cut(s) 136
NspV TTCGAA 1 cut(s) 364
PdmI GAANNNNTTC 1 cut(s) 360
PfeI GAWTC 2 cut(s) 260, 701
PleI GAGTC 2 cut(s) 82, 646
PpsI GAGTC 2 cut(s) 82, 646
PpuMI RGGWCCY 1 cut(s) 5
Psp5II RGGWCCY 1 cut(s) 5
PspN4I GGNNCC 2 cut(s) 6, 179
PspPI GGNCC 2 cut(s) 5, 340
PspPPI RGGWCCY 1 cut(s) 5
RsaI GTAC 1 cut(s) 380
RsaNI GTAC 1 cut(s) 379
RseI CAYNNNNRTG 1 cut(s) 131
SaqAI TTAA 3 cut(s) 510, 540, 627
Sau3AI GATC 1 cut(s) 232
Sau96I GGNCC 2 cut(s) 5, 340
SchI GAGTC 2 cut(s) 83, 647
SfaNI GCATC 3 cut(s) 195, 342, 670
SfuI TTCGAA 1 cut(s) 364
SinI GGWCC 1 cut(s) 5
SmiMI CAYNNNNRTG 1 cut(s) 131
SmlI CTYRAG 1 cut(s) 344
SmoI CTYRAG 1 cut(s) 344
Sse9I AATT 1 cut(s) 484
SsiI CCGC 3 cut(s) 274, 307, 319
SspMI CTAG 3 cut(s) 426, 635, 716
TaaI ACNGT 2 cut(s) 205, 492
TaqI TCGA 7 cut(s) 189, 258, 288, 364, 501, 561, 641
TasI AATT 1 cut(s) 484
TfiI GAWTC 2 cut(s) 260, 701
Tru1I TTAA 3 cut(s) 510, 540, 627
Tru9I TTAA 3 cut(s) 510, 540, 627
TspDTI ATGAA 4 cut(s) 75, 384, 593, 741
VpaK11BI GGWCC 1 cut(s) 5
XbaI TCTAGA 2 cut(s) 634, 715
XmnI GAANNNNTTC 1 cut(s) 360
XspI CTAG 3 cut(s) 426, 635, 716
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.