Rorug06G0139800

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0012981
Physical Location & Seq
Forward (+)
19784617 .. 19789628
5012 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0139800.1

Sequence Viewer

Length: 1524 bp
ATGGCGAAGAAAAATGGCGGTGGCGATCCACCAGCTGAGGAGGAAGAGGAGGAAGAGGAGAACCACGGTGGCGCTGACGGCGGTGCTTCACCGCAGAAAGAAACAGACGACGACGGCGAGAACAAGAAGAAGAAGAAGCATTCAGATCCGGAGCTGCTGATGTGCTTGCTCCAACCTTGTCCCGCAGACTCCGATCCCGAATACATCGGCATTCGTCGCCTCCTTCTCTCTCGCAAGTCTCAGTCCTCCATTCTTCGCCGCCTAGATTGGAGATGCAATGGTAAGGGCTACGTGGCGTTCCGGAATTACATTCGGAGGCCGAGGGATTGGGAGAAGGATAGTCTGCGGACTCCGAGCCTCCAGACCACTCCGGGAAACAGTGGGCGATGGATTCAACCTCCGAGTCCACTCTCACTTTTGTGTGACGTCGAGAGCTGGACTCCTGGCAGGGATGTGCATAATGGCAGTCAACCTTCATCTCGTAGAAGTTTTGGCTCGAGTACTAGTGATAATGACCGCCTGCGTCGACAGGGTGAACAACCTGCATACTCTTTTGTAGGAATGCATTGCATCTTTGATCAGTGCAGAGCCTCGGTTACTGTTTTGAAGTTTGGGCACATGAGCTCAGATCTGCTTGCGTATGGAGCATCAGATGGAACCTTGACAGTATGCACTGTCTCTGATCCTCCTTCAATTCTCAAGCATCTGAATGGTCACTCCAAAGATGTTACAGACTTTGATTTCTCGTCAAACAATCAGTACATTGCATCCTCATCGTTGGATAAAACTGTGAGAGTATGGGAGATTTCAAAAGGCCTATGCATAAGAGTAATATATGGAGTTTCTCCACAACTGTGTATTCGTTTTCACCCTGTAAATAACAACTTCCTTTCAGTTGGCAATGCAGACAAAGAAATTACGGTTTTCAATTTCAGTACTGGGAGGACCATTCATAAAACATCATTTGACAGTCAAGTTACCGCTATGGACCATGCTCACACTGGACAGATCATTTTCTGTGGAGATGGGACGGGGTGTATATATTCTGTCAGTATGAATTCTCACACAGGAGTATTATCCCGCTCTCATCGGCATCGAAGTAGCAGCAGGCAGAAATCTCCAGTCACAACTGTGGAGTACCGAAGTTTTTCTATGTTGGCTCGGGGCCCTGTGTTGCTGACATGTACTCAAGATGGAAGTTTGTCTTTCTTCAGTGTTGCGCTGGAAATACAAGGTTATTTGACTCTTCGTTGCTCACTCAAATTAACTCCACGAATATACAATATCCGGGCTTCCTTTTGTCCTCTGCTTTCCCTTGAAAAAGGAGAATATATAGTTGCCGGAAGTGAAGATTCAAATGTCTACTTCTACGACTTAACTCGGCCAAAGCATACATGTGTAAACAAGCTACAGGGTCATCGATTTCCTGTTATTGGGGTTGCTTGGAACCATGGAGAGAATTTATTAGCATCATCTGATTTCTATGGTACGGTTATTGTATGGAAGAGATCAAAGGCAGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR_2 PPR_3

Protein Analysis

507

Amino Acids

56.34

Weight (kDa)

8.53

Isoelectric Point (pI)

55.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_WDR3_1st PF25173 189 - 280 1.3e-13 WDR3 first beta-propeller domain
WD40_MABP1-WDR62_2nd PF24782 192 - 348 3.4e-06 MABP1/WDR62 second WD40 domain
Beta-prop_EML_2 PF23414 193 - 272 1.3e-08 Echinoderm microtubule-associated protein second beta-propeller
WD40_WDHD1_1st PF24817 194 - 365 2.8e-14 WDHD1 first WD40 domain
Beta-prop_WDR5 PF25175 195 - 502 2.3e-33 WDR5 beta-propeller domain
WDR55 PF24796 195 - 405 4.3e-11 WDR55
Beta-prop_WDR36-Utp21_2nd PF25168 198 - 312 1.2e-11 WDR36/Utp21 second beta-propeller domain
WD40_Prp19 PF24814 198 - 354 7.3e-15 Prp19 WD40 domain
WD40_CDC20-Fz PF24807 201 - 374 1.2e-15 CDC20/Fizzy WD40 domain
Beta-prop_THOC3 PF25174 208 - 365 1.1e-21 THOC3 beta-propeller domain
Beta-prop_CAF1B_HIR1 PF24105 232 - 327 3.9e-09 CAF1B/HIR1 beta-propeller domain
WD40 PF00400 233 - 267 3.8e-10 WD domain, G-beta repeat
Beta-prop_EML PF23409 234 - 489 1.4e-06 Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_WDR3_2nd PF25172 242 - 348 1.2e-07 WDR3 second beta-propeller domain
Beta-prop_EML_2 PF23414 288 - 411 1e-05 Echinoderm microtubule-associated protein second beta-propeller
WD40_CDC20-Fz PF24807 439 - 505 2.8e-09 CDC20/Fizzy WD40 domain
WD40_WDHD1_1st PF24817 440 - 504 6.6e-07 WDHD1 first WD40 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0012981)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G09315
fragaria_vesca FvH4_2g17040
malus_domestica MD10G1143100.v1.1
prunus_persica Prupe.8G182100_v2.0.a1 Prupe.8G182100_v2.0.a1
pyrus_communis pycom05g13780 pycom10g12300
rosa_chinensis RchiOBHm_Chr6g0281221
rosa_laevigata RLG00000012983
rosa_multiflora Rmu_sc0000847.1_g000051 Rmu_sc0027531.1_g000002
rosa_roxburghii Rroxscaffold_7G00187210
rosa_rugosa Rorug06G0139800
rosa_samantha Rh6AG251700 Rh6BG255000 Rh6CG254500 Rh6DG246300
rosa_wichuraiana Rw6G021860

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 429
Acc36I ACCTGC 2 cut(s) 550, 1508
AccBSI CCGCTC 1 cut(s) 1083
AccI GTMKAC 2 cut(s) 526, 1362
AccIII TCCGGA 2 cut(s) 148, 300
AciI CCGC 9 cut(s) 18, 81, 92, 183, 259, 346, 517, 981, 1081
AclWI GGATC 4 cut(s) 20, 140, 188, 677
AcoI YGGCCR 1 cut(s) 1382
AcsI RAATTY 2 cut(s) 1057, 1459
AcuI CTGAAG 1 cut(s) 1195
AcyI GRCGYC 1 cut(s) 426
AfaI GTAC 6 cut(s) 502, 761, 937, 1139, 1186, 1489
AfiI CCNNNNNNNGG 1 cut(s) 1433
AflIII ACRYGT 2 cut(s) 1181, 1394
AgsI TTSAA 7 cut(s) 395, 607, 693, 810, 928, 1319, 1356
AhlI ACTAGT 1 cut(s) 503
AjnI CCWGG 1 cut(s) 442
AleI CACNNNNGTG 3 cut(s) 418, 853, 1130
AluBI AGCT 5 cut(s) 35, 154, 435, 624, 1408
AluI AGCT 5 cut(s) 35, 154, 435, 624, 1408
Alw21I GWGCWC 1 cut(s) 626
Alw26I GTCTC 2 cut(s) 243, 682
AlwI GGATC 4 cut(s) 20, 140, 188, 677
Ama87I CYCGRG 2 cut(s) 496, 1161
Aor13HI TCCGGA 2 cut(s) 148, 300
AoxI GGCC 4 cut(s) 317, 814, 1165, 1382
ApaI GGGCCC 1 cut(s) 1169
ApeKI GCWGC 2 cut(s) 154, 1104
ApoI RAATTY 2 cut(s) 1057, 1459
Asp700I GAANNNNTTC 1 cut(s) 1147
AspLEI GCGC 2 cut(s) 74, 1222
AspS9I GGNCC 4 cut(s) 945, 988, 1165, 1166
AsuC2I CCSGG 2 cut(s) 372, 1289
AsuHPI GGTGA 3 cut(s) 81, 545, 860
AvaI CYCGRG 2 cut(s) 496, 1161
AvaII GGWCC 2 cut(s) 945, 988
BaeGI GKGCMC 2 cut(s) 618, 1169
BanII GRGCYC 2 cut(s) 626, 1169
Bbv12I GWGCWC 1 cut(s) 626
BbvCI CCTCAGC 1 cut(s) 36
BbvI GCAGC 2 cut(s) 141, 1116
BccI CCATC 4 cut(s) 381, 647, 1019, 1187
BceAI ACGGC 2 cut(s) 94, 130
BcgI CGANNNNNNTGC 2 cut(s) 756, 790
BciT130I CCWGG 1 cut(s) 444
BclI TGATCA 1 cut(s) 577
BcnI CCSGG 2 cut(s) 372, 1289
BcoDI GTCTC 2 cut(s) 243, 682
BcuI ACTAGT 1 cut(s) 503
BfaI CTAG 2 cut(s) 263, 504
BfmI CTRYAG 1 cut(s) 1409
BfoI RGCGCY 1 cut(s) 75
BfuAI ACCTGC 2 cut(s) 550, 1508
BglII AGATCT 1 cut(s) 628
BisI GCNGC 3 cut(s) 155, 259, 1105
BlsI GCNGC 3 cut(s) 156, 260, 1106
BmcAI AGTACT 2 cut(s) 502, 937
Bme1390I CCNGG 3 cut(s) 372, 444, 1289
Bme18I GGWCC 2 cut(s) 945, 988
BmeT110I CYCGRG 2 cut(s) 496, 1161
BmgT120I GGNCC 4 cut(s) 945, 988, 1165, 1166
BmiI GGNNCC 4 cut(s) 658, 1166, 1167, 1448
BmrFI CCNGG 3 cut(s) 372, 444, 1289
BmrI ACTGGG 1 cut(s) 948
BmsI GCATC 7 cut(s) 263, 579, 656, 712, 776, 1102, 1478
BmuI ACTGGG 1 cut(s) 948
BplI GAGNNNNNCTC 2 cut(s) 424, 456
BpmI CTGGAG 2 cut(s) 344, 1104
Bpu10I CCTNAGC 1 cut(s) 36
BpuEI CTTGAG 2 cut(s) 683, 1173
BpuMI CCSGG 2 cut(s) 372, 1289
Bsa29I ATCGAT 1 cut(s) 1420
BsaAI YACGTR 1 cut(s) 292
BsaHI GRCGYC 1 cut(s) 426
BsaJI CCNNGG 4 cut(s) 64, 320, 591, 1450
BsaWI WCCGGW 2 cut(s) 148, 300
Bsc4I CCNNNNNNNGG 1 cut(s) 1433
Bse1I ACTGG 3 cut(s) 943, 1006, 1121
Bse3DI GCAATG 4 cut(s) 283, 565, 762, 907
BseAI TCCGGA 2 cut(s) 148, 300
BseBI CCWGG 1 cut(s) 444
BseCI ATCGAT 1 cut(s) 1420
BseDI CCNNGG 4 cut(s) 64, 320, 591, 1450
BseGI GGATG 2 cut(s) 457, 767
BseLI CCNNNNNNNGG 1 cut(s) 1433
BseMI GCAATG 4 cut(s) 283, 565, 762, 907
BseMII CTCAG 3 cut(s) 27, 254, 639
BseNI ACTGG 3 cut(s) 943, 1006, 1121
BseRI GAGGAG 3 cut(s) 53, 62, 71
BseSI GKGCMC 2 cut(s) 618, 1169
BseXI GCAGC 2 cut(s) 141, 1116
BsgI GTGCAG 1 cut(s) 604
BshFI GGCC 4 cut(s) 319, 816, 1167, 1384
BshVI ATCGAT 1 cut(s) 1420
BsiHKAI GWGCWC 1 cut(s) 626
BsiHKCI CYCGRG 2 cut(s) 496, 1161
BsiSI CCGG 5 cut(s) 149, 301, 371, 1288, 1341
BslFI GGGAC 2 cut(s) 165, 1042
BslI CCNNNNNNNGG 1 cut(s) 1433
BsmAI GTCTC 2 cut(s) 243, 682
BsmFI GGGAC 2 cut(s) 165, 1042
BsmI GAATGC 3 cut(s) 139, 210, 567
BsnI GGCC 4 cut(s) 319, 816, 1167, 1384
BsoBI CYCGRG 2 cut(s) 496, 1161
Bsp120I GGGCCC 1 cut(s) 1165
Bsp1286I GDGCHC 3 cut(s) 618, 626, 1169
Bsp13I TCCGGA 2 cut(s) 148, 300
Bsp143I GATC 8 cut(s) 25, 145, 193, 577, 628, 682, 1008, 1508
Bsp19I CCATGG 1 cut(s) 1450
BspACI CCGC 9 cut(s) 18, 81, 92, 183, 259, 346, 517, 981, 1081
BspANI GGCC 4 cut(s) 319, 816, 1167, 1384
BspCNI CTCAG 3 cut(s) 28, 253, 638
BspDI ATCGAT 1 cut(s) 1420
BspEI TCCGGA 2 cut(s) 148, 300
BspLI GGNNCC 4 cut(s) 658, 1166, 1167, 1448
BspMI ACCTGC 2 cut(s) 550, 1508
BspPI GGATC 4 cut(s) 20, 140, 188, 677
BsrBI CCGCTC 1 cut(s) 1083
BsrDI GCAATG 4 cut(s) 283, 565, 762, 907
BsrI ACTGG 3 cut(s) 943, 1006, 1121
BssECI CCNNGG 4 cut(s) 64, 320, 591, 1450
BssMI GATC 8 cut(s) 25, 145, 193, 577, 628, 682, 1008, 1508
BssNI GRCGYC 1 cut(s) 426
BssT1I CCWWGG 1 cut(s) 1450
Bst2UI CCWGG 1 cut(s) 444
Bst6I CTCTTC 4 cut(s) 39, 48, 1251, 1499
BstACI GRCGYC 1 cut(s) 426
BstBAI YACGTR 1 cut(s) 292
BstC8I GCNNGC 4 cut(s) 167, 521, 636, 1109
BstDEI CTNAG 3 cut(s) 36, 240, 625
BstDSI CCRYGG 2 cut(s) 64, 1450
BstF5I GGATG 2 cut(s) 457, 767
BstH2I RGCGCY 1 cut(s) 75
BstHHI GCGC 2 cut(s) 74, 1222
BstKTI GATC 8 cut(s) 28, 148, 196, 580, 631, 685, 1011, 1511
BstMAI GTCTC 2 cut(s) 243, 682
BstMBI GATC 8 cut(s) 25, 145, 193, 577, 628, 682, 1008, 1508
BstMWI GCNNNNNNNGC 3 cut(s) 78, 216, 644
BstNI CCWGG 1 cut(s) 444
BstNSI RCATGY 2 cut(s) 1185, 1398
BstSCI CCNGG 3 cut(s) 370, 442, 1287
BstSFI CTRYAG 1 cut(s) 1409
BstSLI GKGCMC 2 cut(s) 618, 1169
BstV1I GCAGC 2 cut(s) 141, 1116
BstX2I RGATCY 2 cut(s) 145, 628
BstYI RGATCY 2 cut(s) 145, 628
Bsu15I ATCGAT 1 cut(s) 1420
BsuRI GGCC 4 cut(s) 319, 816, 1167, 1384
BsuTUI ATCGAT 1 cut(s) 1420
BtgI CCRYGG 2 cut(s) 64, 1450
BtgZI GCGATG 1 cut(s) 400
BtsCI GGATG 2 cut(s) 457, 767
BtsIMutI CAGTG 5 cut(s) 385, 587, 672, 999, 1219
BveI ACCTGC 2 cut(s) 550, 1508
Cac8I GCNNGC 4 cut(s) 167, 521, 636, 1109
CfoI GCGC 2 cut(s) 74, 1222
Cfr13I GGNCC 4 cut(s) 945, 988, 1165, 1166
ClaI ATCGAT 1 cut(s) 1420
CseI GACGC 1 cut(s) 512
Csp6I GTAC 6 cut(s) 501, 760, 936, 1138, 1185, 1488
CviAII CATG 5 cut(s) 619, 992, 1182, 1395, 1451
CviQI GTAC 6 cut(s) 501, 760, 936, 1138, 1185, 1488
DdeI CTNAG 3 cut(s) 36, 240, 625
DpnI GATC 8 cut(s) 27, 147, 195, 579, 630, 684, 1010, 1510
DpnII GATC 8 cut(s) 25, 145, 193, 577, 628, 682, 1008, 1508
EaeI YGGCCR 1 cut(s) 1382
Eam1104I CTCTTC 4 cut(s) 39, 48, 1251, 1499
EarI CTCTTC 4 cut(s) 39, 48, 1251, 1499
Ecl136II GAGCTC 1 cut(s) 624
Eco130I CCWWGG 1 cut(s) 1450
Eco147I AGGCCT 1 cut(s) 816
Eco24I GRGCYC 2 cut(s) 626, 1169
Eco47I GGWCC 2 cut(s) 945, 988
Eco53kI GAGCTC 1 cut(s) 624
Eco57I CTGAAG 1 cut(s) 1195
Eco88I CYCGRG 2 cut(s) 496, 1161
EcoICRI GAGCTC 1 cut(s) 624
EcoO109I RGGNCCY 2 cut(s) 1165, 1166
EcoRI GAATTC 1 cut(s) 1057
EcoRII CCWGG 1 cut(s) 442
EcoT14I CCWWGG 1 cut(s) 1450
EcoT22I ATGCAT 2 cut(s) 567, 824
EcoT38I GRGCYC 2 cut(s) 626, 1169
ErhI CCWWGG 1 cut(s) 1450
FaeI CATG 5 cut(s) 622, 995, 1185, 1398, 1454
FalI AAGNNNNNCTT 2 cut(s) 1189, 1221
FaqI GGGAC 2 cut(s) 165, 1042
FatI CATG 5 cut(s) 618, 991, 1181, 1394, 1450
FauI CCCGC 2 cut(s) 190, 1088
FbaI TGATCA 1 cut(s) 577
FblI GTMKAC 2 cut(s) 526, 1362
Fnu4HI GCNGC 3 cut(s) 155, 259, 1105
FokI GGATG 2 cut(s) 464, 754
FriOI GRGCYC 2 cut(s) 626, 1169
Fsp4HI GCNGC 3 cut(s) 155, 259, 1105
FspBI CTAG 2 cut(s) 263, 504
GlaI GCGC 2 cut(s) 73, 1221
GluI GCNGC 3 cut(s) 155, 259, 1105
GsuI CTGGAG 2 cut(s) 344, 1104
HaeII RGCGCY 1 cut(s) 75
HaeIII GGCC 4 cut(s) 319, 816, 1167, 1384
HapII CCGG 5 cut(s) 149, 301, 371, 1288, 1341
HgaI GACGC 1 cut(s) 512
HhaI GCGC 2 cut(s) 74, 1222
Hin1I GRCGYC 1 cut(s) 426
Hin1II CATG 5 cut(s) 622, 995, 1185, 1398, 1454
Hin6I GCGC 2 cut(s) 72, 1220
HinP1I GCGC 2 cut(s) 72, 1220
HincII GTYRAC 2 cut(s) 470, 527
HindII GTYRAC 2 cut(s) 470, 527
HinfI GANTC 7 cut(s) 188, 349, 391, 403, 439, 1243, 1352
HpaII CCGG 5 cut(s) 149, 301, 371, 1288, 1341
HphI GGTGA 3 cut(s) 81, 545, 860
Hpy166II GTNNAC 6 cut(s) 407, 470, 527, 536, 1363, 1402
Hpy188III TCNNGA 6 cut(s) 149, 197, 301, 361, 430, 1190
Hpy8I GTNNAC 6 cut(s) 407, 470, 527, 536, 1363, 1402
Hpy99I CGWCG 5 cut(s) 113, 116, 219, 431, 528
HpyAV CCTTC 4 cut(s) 233, 328, 483, 699
HpyCH4IV ACGT 2 cut(s) 291, 426
HpyF10VI GCNNNNNNNGC 3 cut(s) 78, 216, 644
HpyF3I CTNAG 3 cut(s) 36, 240, 625
HpySE526I ACGT 2 cut(s) 291, 426
Hsp92I GRCGYC 1 cut(s) 426
Hsp92II CATG 5 cut(s) 622, 995, 1185, 1398, 1454
HspAI GCGC 2 cut(s) 72, 1220
Kpn2I TCCGGA 2 cut(s) 148, 300
Ksp22I TGATCA 1 cut(s) 577
Kzo9I GATC 8 cut(s) 25, 145, 193, 577, 628, 682, 1008, 1508
LmnI GCTCC 3 cut(s) 151, 174, 644
Lsp1109I GCAGC 2 cut(s) 141, 1116
LweI GCATC 7 cut(s) 263, 579, 656, 712, 776, 1102, 1478
MaeI CTAG 2 cut(s) 263, 504
MaeII ACGT 2 cut(s) 291, 426
MaeIII GTNAC 6 cut(s) 422, 595, 713, 727, 976, 1123
MalI GATC 8 cut(s) 27, 147, 195, 579, 630, 684, 1010, 1510
MbiI CCGCTC 1 cut(s) 1083
MboI GATC 8 cut(s) 25, 145, 193, 577, 628, 682, 1008, 1508
MflI RGATCY 2 cut(s) 145, 628
MhlI GDGCHC 3 cut(s) 618, 626, 1169
MluCI AATT 7 cut(s) 304, 693, 915, 928, 1057, 1262, 1459
MlyI GAGTC 5 cut(s) 182, 343, 412, 433, 1237
MmeI TCCRAC 2 cut(s) 196, 759
Mph1103I ATGCAT 2 cut(s) 567, 824
MroI TCCGGA 2 cut(s) 148, 300
MroXI GAANNNNTTC 1 cut(s) 1147
MseI TTAA 3 cut(s) 1265, 1376, 1522
MslI CAYNNNNRTG 5 cut(s) 418, 708, 853, 1130, 1395
MspA1I CMGCKG 1 cut(s) 35
MspI CCGG 5 cut(s) 149, 301, 371, 1288, 1341
MspR9I CCNGG 3 cut(s) 372, 444, 1289
Mva1269I GAATGC 3 cut(s) 139, 210, 567
MvaI CCWGG 1 cut(s) 444
MwoI GCNNNNNNNGC 3 cut(s) 78, 216, 644
NciI CCSGG 2 cut(s) 372, 1289
NcoI CCATGG 1 cut(s) 1450
NdeII GATC 8 cut(s) 25, 145, 193, 577, 628, 682, 1008, 1508
NlaIII CATG 5 cut(s) 622, 995, 1185, 1398, 1454
NlaIV GGNNCC 4 cut(s) 658, 1166, 1167, 1448
NmeAIII GCCGAG 2 cut(s) 345, 1360
NmuCI GTSAC 3 cut(s) 422, 713, 1123
NsiI ATGCAT 2 cut(s) 567, 824
NspI RCATGY 2 cut(s) 1185, 1398
OliI CACNNNNGTG 3 cut(s) 418, 853, 1130
PaeR7I CTCGAG 1 cut(s) 496
PceI AGGCCT 1 cut(s) 816
PciI ACATGT 2 cut(s) 1181, 1394
PcsI WCGNNNNNNNCGW 1 cut(s) 114
PctI GAATGC 3 cut(s) 139, 210, 567
PdmI GAANNNNTTC 1 cut(s) 1147
PfeI GAWTC 2 cut(s) 391, 1352
PfoI TCCNGGA 1 cut(s) 370
PkrI GCNGC 3 cut(s) 156, 260, 1106
PleI GAGTC 5 cut(s) 182, 343, 411, 433, 1237
PpsI GAGTC 5 cut(s) 182, 343, 411, 433, 1237
Ppu21I YACGTR 1 cut(s) 292
PscI ACATGT 2 cut(s) 1181, 1394
Psp124BI GAGCTC 1 cut(s) 626
Psp6I CCWGG 1 cut(s) 442
PspGI CCWGG 1 cut(s) 442
PspN4I GGNNCC 4 cut(s) 658, 1166, 1167, 1448
PspOMI GGGCCC 1 cut(s) 1165
PspPI GGNCC 4 cut(s) 945, 988, 1165, 1166
PspXI VCTCGAGB 1 cut(s) 496
PsuI RGATCY 2 cut(s) 145, 628
PvuII CAGCTG 1 cut(s) 35
RsaI GTAC 6 cut(s) 502, 761, 937, 1139, 1186, 1489
RsaNI GTAC 6 cut(s) 501, 760, 936, 1138, 1185, 1488
RseI CAYNNNNRTG 5 cut(s) 418, 708, 853, 1130, 1395
SacI GAGCTC 1 cut(s) 626
SalI GTCGAC 1 cut(s) 525
SaqAI TTAA 3 cut(s) 1265, 1376, 1522
SatI GCNGC 3 cut(s) 155, 259, 1105
Sau3AI GATC 8 cut(s) 25, 145, 193, 577, 628, 682, 1008, 1508
Sau96I GGNCC 4 cut(s) 945, 988, 1165, 1166
ScaI AGTACT 2 cut(s) 502, 937
SchI GAGTC 5 cut(s) 182, 343, 412, 433, 1237
ScrFI CCNGG 3 cut(s) 372, 444, 1289
SduI GDGCHC 3 cut(s) 618, 626, 1169
SfaNI GCATC 7 cut(s) 263, 579, 656, 712, 776, 1102, 1478
SfcI CTRYAG 1 cut(s) 1409
Sfr274I CTCGAG 1 cut(s) 496
SinI GGWCC 2 cut(s) 945, 988
SlaI CTCGAG 1 cut(s) 496
SmiMI CAYNNNNRTG 5 cut(s) 418, 708, 853, 1130, 1395
SmlI CTYRAG 3 cut(s) 496, 698, 1188
SmoI CTYRAG 3 cut(s) 496, 698, 1188
SpeI ACTAGT 1 cut(s) 503
Sse9I AATT 7 cut(s) 304, 693, 915, 928, 1057, 1262, 1459
SseBI AGGCCT 1 cut(s) 816
SsiI CCGC 9 cut(s) 18, 81, 92, 183, 259, 346, 517, 981, 1081
SspMI CTAG 2 cut(s) 263, 504
SstI GAGCTC 1 cut(s) 626
StuI AGGCCT 1 cut(s) 816
StyD4I CCNGG 3 cut(s) 370, 442, 1287
StyI CCWWGG 1 cut(s) 1450
TaiI ACGT 2 cut(s) 294, 429
TaqI TCGA 5 cut(s) 429, 497, 526, 1096, 1420
TasI AATT 7 cut(s) 304, 693, 915, 928, 1057, 1262, 1459
TatI WGTACW 4 cut(s) 500, 759, 935, 1184
TauI GCSGC 1 cut(s) 261
TfiI GAWTC 2 cut(s) 391, 1352
Tru1I TTAA 3 cut(s) 1265, 1376, 1522
Tru9I TTAA 3 cut(s) 1265, 1376, 1522
TscAI CASTG 5 cut(s) 385, 587, 679, 1006, 1219
TseFI GTSAC 3 cut(s) 422, 713, 1123
TseI GCWGC 2 cut(s) 154, 1104
Tsp45I GTSAC 3 cut(s) 422, 713, 1123
TspDTI ATGAA 3 cut(s) 465, 941, 1070
TspRI CASTG 5 cut(s) 385, 587, 679, 1006, 1219
VpaK11BI GGWCC 2 cut(s) 945, 988
XapI RAATTY 2 cut(s) 1057, 1459
XceI RCATGY 2 cut(s) 1185, 1398
XcmI CCANNNNNNNNNTGG 1 cut(s) 998
XhoI CTCGAG 1 cut(s) 496
XmiI GTMKAC 2 cut(s) 526, 1362
XmnI GAANNNNTTC 1 cut(s) 1147
XspI CTAG 2 cut(s) 263, 504
ZraI GACGTC 1 cut(s) 427
ZrmI AGTACT 2 cut(s) 502, 937
Zsp2I ATGCAT 2 cut(s) 567, 824
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.