pycom10g12460

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0012429
Physical Location & Seq
Forward (+)
15941096 .. 15942676
1581 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1188 bp
ATGCCATCGTTTCAGGGTCAGGTTTCCAAGCACATATTGTCCACCATTTCTAACCCTCTTCGCCAATCAACGAGCTTGTCGATGAAGCTACGTCGTTTTAGCACCACCGCCGATCGTGGCGGTGAAATGGAAGCACCACTGGAGCAACAGCCGCCGGAGCCCATACCCAATAGGCCCTTGAGAGGCCAAAGACCATCAAATCCTCAAACTTCCAATCTTCAGTTTCTCAACAAAAACTCGCCCATTTCAGAGAAAAGGAGAGAGAGGCCTTCTTCTCCGCCATTGCAGGACAGCAGCTTTCTCGAAAAGCTGAAGATGGGTCTCGACAAGTCGAAGAGGGAGGAGCCTCAGGAAGTTCCCGAGCCGCCACAGCCGCCCGAAGAAGCGGACCAGATATTCAAGAAGATGAAGGAGACTGGTCTCATTCCCAATGCCGTGGCAATGCTTGACGGGCTCTGCAAAGATGGGCTGGTCCAGGAGGCTATGAAGCTTTTCGGGTCGATGCGTGAAAAGGGCACGATTCCTGAGATTGTTATATACACTGCGGTGGTTGATGGGTTTTGCAAAGCACAGAAACTTGAAGATGCTAAGAGAATTTTCAGGAAGATGCAGAGCAATGGGATTGTTCCCAATGCTTTTAGTTACACTGTGTTGATACAGGGGTTGTATCGGGCTAATATGTTGGAGGATGCTGTTGAATTTTGTAGCGAAATGTTGGAAGCCGGTCACTCGCCCAATGTAACTACTTTTGTGGGGCTGATTGATATGGTTTGTAAGGAGAAGGATATGGAAGAAGCTGAGAGTGTTATTGGGAAGTTGAAGCAGAAGGGGTATTTGGTTAATGAGAAGGCTGTTAAAGAGTTTTTGGACAAGAAGGCACCATTTTCGCCGAGGATTTGGGAAGCTATCTTTGGGAAGAATAAATCGCAGAGACGTGTTGCCCCACTGTATGAGTGGCGGCATGAGAAGAACATAAATGTCACCCTTGGAAAATACTCTGTTAATAATGGTCACGATTCCTTGTGCTTTGTATGGTTTTCCAGGATCTTTAAGCATAAGCTTTTTTTATCATATTTTCTTACAGTTCACATTTGTCAATTCAATCATTCCGACAAAATCGAAAATGCTTATTGGCTCTTTGAAGAAAACATAGTTGAAAAGGCTAAGGAGGGGAGATGCTGGTGCTAG

Protein Analysis

396

Amino Acids

45.17

Weight (kDa)

8.83

Isoelectric Point (pI)

53.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR PF01535 147 - 172 1.7e-06 PPR repeat
PPR_3 PF13812 164 - 220 3.8e-08 Pentatricopeptide repeat domain
PPR_1 PF12854 174 - 204 2.4e-07 PPR repeat
PPR_2 PF13041 175 - 223 8.6e-15 PPR repeat family
PPR PF01535 178 - 208 6.3e-07 PPR repeat
PPR_1 PF12854 206 - 238 5.5e-07 PPR repeat
PPR_2 PF13041 213 - 259 1.4e-10 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012429)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G38150 AT4G38150 AT4G38150
fragaria_vesca FvH4_2g17180
malus_domestica MD05G1145100.v1.1
prunus_persica Prupe.8G183200_v2.0.a1
pyrus_communis pycom05g14030 pycom07g10800 pycom10g12460
rosa_chinensis RchiOBHm_Chr6g0281671
rosa_laevigata RLG00000012952
rosa_multiflora Rmu_co8238379.1_g000001 Rmu_sc0019758.1_g000001
rosa_rugosa Rorug06G0142300
rosa_samantha Rh6AG255000 Rh6BG258400 Rh6CG257200 Rh6DG249400
rosa_wichuraiana Rw6G022130

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 877
AciI CCGC 9 cut(s) 108, 120, 152, 278, 365, 374, 386, 545, 958
AclWI GGATC 1 cut(s) 1052
AcsI RAATTY 2 cut(s) 594, 698
AcuI CTGAAG 2 cut(s) 203, 332
AfiI CCNNNNNNNGG 1 cut(s) 182
AflIII ACRYGT 1 cut(s) 934
AgsI TTSAA 7 cut(s) 400, 581, 698, 820, 1102, 1142, 1157
AjiI CACGTC 1 cut(s) 935
AjnI CCWGG 2 cut(s) 474, 1040
AjuI GAANNNNNNNTTGG 4 cut(s) 818, 850, 894, 926
AleI CACNNNNGTG 1 cut(s) 545
AluBI AGCT 8 cut(s) 75, 88, 297, 310, 490, 797, 905, 1060
AluI AGCT 8 cut(s) 75, 88, 297, 310, 490, 797, 905, 1060
Alw26I GTCTC 4 cut(s) 326, 407, 425, 925
AlwI GGATC 1 cut(s) 1052
Ama87I CYCGRG 1 cut(s) 359
AoxI GGCC 3 cut(s) 173, 184, 266
ApeKI GCWGC 1 cut(s) 294
ApoI RAATTY 2 cut(s) 594, 698
Asp700I GAANNNNTTC 1 cut(s) 491
AspS9I GGNCC 3 cut(s) 174, 388, 472
AsuHPI GGTGA 2 cut(s) 134, 973
AvaI CYCGRG 1 cut(s) 359
AvaII GGWCC 2 cut(s) 388, 472
AxyI CCTNAGG 1 cut(s) 348
BaeGI GKGCMC 1 cut(s) 518
BaeI ACNNNNGTAYC 2 cut(s) 647, 680
BanI GGYRCC 1 cut(s) 877
BanII GRGCYC 2 cut(s) 162, 456
BbvI GCAGC 1 cut(s) 306
BccI CCATC 5 cut(s) 13, 202, 310, 458, 548
BceAI ACGGC 1 cut(s) 419
BcgI CGANNNNNNTGC 4 cut(s) 283, 317, 867, 901
BciT130I CCWGG 2 cut(s) 476, 1042
BcoDI GTCTC 4 cut(s) 326, 407, 425, 925
BfaI CTAG 1 cut(s) 1186
BisI GCNGC 5 cut(s) 152, 295, 365, 374, 959
BlsI GCNGC 5 cut(s) 153, 296, 366, 375, 960
Bme1390I CCNGG 2 cut(s) 476, 1042
Bme18I GGWCC 2 cut(s) 388, 472
BmeT110I CYCGRG 1 cut(s) 359
BmgBI CACGTC 1 cut(s) 935
BmgT120I GGNCC 3 cut(s) 174, 388, 472
BmiI GGNNCC 3 cut(s) 159, 345, 879
BmrFI CCNGG 2 cut(s) 476, 1042
BmsI GCATC 5 cut(s) 492, 574, 597, 679, 1166
BpmI CTGGAG 1 cut(s) 161
Bpu10I CCTNAGC 1 cut(s) 1164
BpuEI CTTGAG 1 cut(s) 199
BsaI GGTCTC 2 cut(s) 326, 425
BsaJI CCNNGG 3 cut(s) 435, 890, 985
Bsc4I CCNNNNNNNGG 1 cut(s) 182
Bse118I RCCGGY 1 cut(s) 722
Bse1I ACTGG 2 cut(s) 144, 421
Bse21I CCTNAGG 1 cut(s) 348
Bse3DI GCAATG 3 cut(s) 281, 447, 622
BseBI CCWGG 2 cut(s) 476, 1042
BseDI CCNNGG 3 cut(s) 435, 890, 985
BseGI GGATG 1 cut(s) 694
BseLI CCNNNNNNNGG 1 cut(s) 182
BseMI GCAATG 3 cut(s) 281, 447, 622
BseMII CTCAG 3 cut(s) 362, 516, 789
BseNI ACTGG 2 cut(s) 144, 421
BseRI GAGGAG 1 cut(s) 356
BseSI GKGCMC 1 cut(s) 518
BseXI GCAGC 1 cut(s) 306
Bsh1285I CGRYCG 1 cut(s) 115
BshFI GGCC 3 cut(s) 175, 186, 268
BshNI GGYRCC 1 cut(s) 877
BsiEI CGRYCG 1 cut(s) 115
BsiHKCI CYCGRG 1 cut(s) 359
BsiSI CCGG 2 cut(s) 155, 723
BslI CCNNNNNNNGG 1 cut(s) 182
BsmAI GTCTC 4 cut(s) 326, 407, 425, 925
BsmBI CGTCTC 1 cut(s) 925
BsnI GGCC 3 cut(s) 175, 186, 268
Bso31I GGTCTC 2 cut(s) 326, 425
BsoBI CYCGRG 1 cut(s) 359
Bsp1286I GDGCHC 3 cut(s) 162, 456, 518
Bsp143I GATC 2 cut(s) 112, 1044
BspACI CCGC 9 cut(s) 108, 120, 152, 278, 365, 374, 386, 545, 958
BspANI GGCC 3 cut(s) 175, 186, 268
BspCNI CTCAG 3 cut(s) 361, 517, 790
BspLI GGNNCC 3 cut(s) 159, 345, 879
BspPI GGATC 1 cut(s) 1052
BspT107I GGYRCC 1 cut(s) 877
BspTNI GGTCTC 2 cut(s) 326, 425
BsrDI GCAATG 3 cut(s) 281, 447, 622
BsrFI RCCGGY 1 cut(s) 722
BsrI ACTGG 2 cut(s) 144, 421
BssAI RCCGGY 1 cut(s) 722
BssECI CCNNGG 3 cut(s) 435, 890, 985
BssMI GATC 2 cut(s) 112, 1044
BssT1I CCWWGG 1 cut(s) 985
Bst2UI CCWGG 2 cut(s) 476, 1042
Bst4CI ACNGT 3 cut(s) 649, 948, 1084
Bst6I CTCTTC 2 cut(s) 63, 329
BstDEI CTNAG 5 cut(s) 348, 525, 588, 798, 1164
BstDSI CCRYGG 1 cut(s) 435
BstF5I GGATG 1 cut(s) 694
BstKTI GATC 2 cut(s) 115, 1047
BstMAI GTCTC 4 cut(s) 326, 407, 425, 925
BstMBI GATC 2 cut(s) 112, 1044
BstMCI CGRYCG 1 cut(s) 115
BstMWI GCNNNNNNNGC 5 cut(s) 151, 157, 370, 373, 451
BstNI CCWGG 2 cut(s) 476, 1042
BstSCI CCNGG 2 cut(s) 474, 1040
BstSLI GKGCMC 1 cut(s) 518
BstV1I GCAGC 1 cut(s) 306
BstX2I RGATCY 1 cut(s) 1044
BstXI CCANNNNNNTGG 1 cut(s) 436
BstYI RGATCY 1 cut(s) 1044
Bsu36I CCTNAGG 1 cut(s) 348
BsuRI GGCC 3 cut(s) 175, 186, 268
BtgI CCRYGG 1 cut(s) 435
BtrI CACGTC 1 cut(s) 935
BtsCI GGATG 1 cut(s) 694
BtsI GCAGTG 1 cut(s) 540
BtsIMutI CAGTG 4 cut(s) 137, 540, 645, 944
Cfr10I RCCGGY 1 cut(s) 722
Cfr13I GGNCC 3 cut(s) 174, 388, 472
CviAII CATG 1 cut(s) 962
DdeI CTNAG 5 cut(s) 348, 525, 588, 798, 1164
DpnI GATC 2 cut(s) 114, 1046
DpnII GATC 2 cut(s) 112, 1044
Eam1104I CTCTTC 2 cut(s) 63, 329
EarI CTCTTC 2 cut(s) 63, 329
EciI GGCGGA 1 cut(s) 267
Eco130I CCWWGG 1 cut(s) 985
Eco147I AGGCCT 1 cut(s) 268
Eco24I GRGCYC 2 cut(s) 162, 456
Eco31I GGTCTC 2 cut(s) 326, 425
Eco47I GGWCC 2 cut(s) 388, 472
Eco57I CTGAAG 2 cut(s) 203, 332
Eco81I CCTNAGG 1 cut(s) 348
Eco88I CYCGRG 1 cut(s) 359
EcoO109I RGGNCCY 1 cut(s) 174
EcoRII CCWGG 2 cut(s) 474, 1040
EcoT14I CCWWGG 1 cut(s) 985
EcoT38I GRGCYC 2 cut(s) 162, 456
ErhI CCWWGG 1 cut(s) 985
Esp3I CGTCTC 1 cut(s) 925
FaeI CATG 1 cut(s) 965
FatI CATG 1 cut(s) 961
Fnu4HI GCNGC 5 cut(s) 152, 295, 365, 374, 959
FokI GGATG 1 cut(s) 701
FriOI GRGCYC 2 cut(s) 162, 456
Fsp4HI GCNGC 5 cut(s) 152, 295, 365, 374, 959
FspBI CTAG 1 cut(s) 1186
GluI GCNGC 5 cut(s) 152, 295, 365, 374, 959
GsuI CTGGAG 1 cut(s) 161
HaeIII GGCC 3 cut(s) 175, 186, 268
HapII CCGG 2 cut(s) 155, 723
Hin1II CATG 1 cut(s) 965
HindIII AAGCTT 2 cut(s) 488, 1058
HinfI GANTC 2 cut(s) 520, 1016
HpaII CCGG 2 cut(s) 155, 723
HphI GGTGA 2 cut(s) 134, 973
Hpy166II GTNNAC 2 cut(s) 42, 1087
Hpy188I TCNGA 2 cut(s) 250, 1111
Hpy188III TCNNGA 8 cut(s) 302, 323, 350, 359, 400, 524, 601, 1013
Hpy8I GTNNAC 2 cut(s) 42, 1087
Hpy99I CGWCG 1 cut(s) 96
HpyAV CCTTC 6 cut(s) 279, 403, 775, 820, 841, 868
HpyCH4III ACNGT 3 cut(s) 649, 948, 1084
HpyCH4IV ACGT 2 cut(s) 91, 934
HpyCH4V TGCA 4 cut(s) 286, 459, 564, 610
HpyF10VI GCNNNNNNNGC 5 cut(s) 151, 157, 370, 373, 451
HpyF3I CTNAG 5 cut(s) 348, 525, 588, 798, 1164
HpySE526I ACGT 2 cut(s) 91, 934
Hsp92II CATG 1 cut(s) 965
Kzo9I GATC 2 cut(s) 112, 1044
LmnI GCTCC 3 cut(s) 142, 157, 343
Lsp1109I GCAGC 1 cut(s) 306
LweI GCATC 5 cut(s) 492, 574, 597, 679, 1166
MaeI CTAG 1 cut(s) 1186
MaeII ACGT 2 cut(s) 91, 934
MaeIII GTNAC 5 cut(s) 641, 725, 739, 979, 1010
MalI GATC 2 cut(s) 114, 1046
MboI GATC 2 cut(s) 112, 1044
MflI RGATCY 1 cut(s) 1044
MhlI GDGCHC 3 cut(s) 162, 456, 518
MluCI AATT 3 cut(s) 594, 698, 1097
MmeI TCCRAC 3 cut(s) 663, 696, 1134
MroXI GAANNNNTTC 1 cut(s) 491
MseI TTAA 4 cut(s) 840, 855, 1002, 1050
MslI CAYNNNNRTG 1 cut(s) 545
MspI CCGG 2 cut(s) 155, 723
MspR9I CCNGG 2 cut(s) 476, 1042
MvaI CCWGG 2 cut(s) 476, 1042
MwoI GCNNNNNNNGC 5 cut(s) 151, 157, 370, 373, 451
NdeII GATC 2 cut(s) 112, 1044
NlaIII CATG 1 cut(s) 965
NlaIV GGNNCC 3 cut(s) 159, 345, 879
NmeAIII GCCGAG 1 cut(s) 915
NmuCI GTSAC 3 cut(s) 725, 979, 1010
OliI CACNNNNGTG 1 cut(s) 545
PceI AGGCCT 1 cut(s) 268
PcsI WCGNNNNNNNCGW 1 cut(s) 77
PdmI GAANNNNTTC 1 cut(s) 491
PfeI GAWTC 2 cut(s) 520, 1016
PfoI TCCNGGA 2 cut(s) 474, 1040
PkrI GCNGC 5 cut(s) 153, 296, 366, 375, 960
Ple19I CGATCG 1 cut(s) 115
Psp6I CCWGG 2 cut(s) 474, 1040
PspGI CCWGG 2 cut(s) 474, 1040
PspN4I GGNNCC 3 cut(s) 159, 345, 879
PspPI GGNCC 3 cut(s) 174, 388, 472
PsuI RGATCY 1 cut(s) 1044
PvuI CGATCG 1 cut(s) 115
RseI CAYNNNNRTG 1 cut(s) 545
SaqAI TTAA 4 cut(s) 840, 855, 1002, 1050
SatI GCNGC 5 cut(s) 152, 295, 365, 374, 959
Sau3AI GATC 2 cut(s) 112, 1044
Sau96I GGNCC 3 cut(s) 174, 388, 472
ScrFI CCNGG 2 cut(s) 476, 1042
SduI GDGCHC 3 cut(s) 162, 456, 518
SfaNI GCATC 5 cut(s) 492, 574, 597, 679, 1166
SinI GGWCC 2 cut(s) 388, 472
SmiMI CAYNNNNRTG 1 cut(s) 545
SmlI CTYRAG 1 cut(s) 178
SmoI CTYRAG 1 cut(s) 178
Sse9I AATT 3 cut(s) 594, 698, 1097
SseBI AGGCCT 1 cut(s) 268
SsiI CCGC 9 cut(s) 108, 120, 152, 278, 365, 374, 386, 545, 958
SspMI CTAG 1 cut(s) 1186
StuI AGGCCT 1 cut(s) 268
StyD4I CCNGG 2 cut(s) 474, 1040
StyI CCWWGG 1 cut(s) 985
TaaI ACNGT 3 cut(s) 649, 948, 1084
TaiI ACGT 2 cut(s) 94, 937
TaqI TCGA 6 cut(s) 80, 303, 324, 332, 500, 1119
TasI AATT 3 cut(s) 594, 698, 1097
TauI GCSGC 4 cut(s) 154, 367, 376, 961
TfiI GAWTC 2 cut(s) 520, 1016
Tru1I TTAA 4 cut(s) 840, 855, 1002, 1050
Tru9I TTAA 4 cut(s) 840, 855, 1002, 1050
TscAI CASTG 4 cut(s) 144, 547, 652, 951
TseFI GTSAC 3 cut(s) 725, 979, 1010
TseI GCWGC 1 cut(s) 294
Tsp45I GTSAC 3 cut(s) 725, 979, 1010
TspDTI ATGAA 3 cut(s) 98, 422, 500
TspRI CASTG 4 cut(s) 144, 547, 652, 951
VpaK11BI GGWCC 2 cut(s) 388, 472
XapI RAATTY 2 cut(s) 594, 698
XcmI CCANNNNNNNNNTGG 1 cut(s) 951
XmnI GAANNNNTTC 1 cut(s) 491
XspI CTAG 1 cut(s) 1186
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.