Rh6CG257200

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6C
OG0012429
Physical Location & Seq
Forward (+)
42894053 .. 42894982
930 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6CG257200.1

Sequence Viewer

Length: 930 bp
ATGCCATCGTTTACGAAGCACATCTTCTCCACAATTTCCAACCCTATTGGCCATTCATCTCAGGCCTCCATTTCCGCTAGCTCGTTGATTAAACTACGTCGTTTTAGCTCCGGTACTGATCGTGGCCGTGAAATGGAAGCGCAGGAGAAACAACCGCCGGAGCCGATACCCAATAGGCCCTTGAGAGGCCAGAGGCCATCGAATCCCCAGACCACTGTTGATAGAAGGAGAGAGAGGAGGAGGGAGAACCCTAATCCGCCATTGCAGGACAGCAGTTTTCTTGAGAAGCTCAAGATGGGTCTTGACAAGTCGAAGAGAGAGAAGCCCCAGGAACCGGCGGAGGAGCCGCCGCAGCAGCAGCCACCGGAGGAGGCCAATGAGATATTCAAGAAGATGAAGGAGACGGGTCTGATTCCCAATGCAGTGGCCATGCTTGATGGGCTCTGCAAAGATGGGCTGGTCCAGGAGGCAATGAAGCTTTTCGGGTCGATGCGCGAAAAGGGTACTATTCCTGAGGTGGTGATATACACTGCTGTGGTGGAAGGGTTTTGCAAAGCCCAGAAGCCTGAAGATGCTAAGAGGATTTTCAGGAAGATGCAGAGCAATGGGATTGTTCCGAATGCGTTTAGTTACAATGTGTTGGTGCAGGGGTTGTGTAGGTGTGATAAGCTGGAGGATGCTGCTATGTTCTGTGGCGAAATGTTGGAAGCCGGTCACTCGCCGAATGTGACTACTTTTGTGGGGTTGGTTGATGGGGTATGCAAGGAAAGTGGCGTGGAAGGAGCTGAAAGTGTGATTGGGAAGTTGAAGCAGAAGGGGTATATGGTTAATGAGAAGGCTGTTAGAGAGTTTTTGGATAAAAGGGCAACGTTTTCGCCAGTGGTTTGGGAAGCTATCTTTGGGAAGAACCACCCAAAGAAACCTTTTTGA

Protein Analysis

309

Amino Acids

34.52

Weight (kDa)

9.12

Isoelectric Point (pI)

54.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR PF01535 143 - 168 1.3e-06 PPR repeat
PPR_3 PF13812 160 - 215 5.4e-09 Pentatricopeptide repeat domain
PPR_1 PF12854 170 - 200 2.3e-06 PPR repeat
PPR_2 PF13041 171 - 220 1.9e-15 PPR repeat family
PPR PF01535 174 - 204 1.8e-06 PPR repeat
PPR_1 PF12854 202 - 234 4.9e-08 PPR repeat
PPR_2 PF13041 206 - 255 6.2e-12 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012429)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G38150 AT4G38150 AT4G38150
fragaria_vesca FvH4_2g17180
malus_domestica MD05G1145100.v1.1
prunus_persica Prupe.8G183200_v2.0.a1
pyrus_communis pycom05g14030 pycom07g10800 pycom10g12460
rosa_chinensis RchiOBHm_Chr6g0281671
rosa_laevigata RLG00000012952
rosa_multiflora Rmu_co8238379.1_g000001 Rmu_sc0019758.1_g000001
rosa_rugosa Rorug06G0142300
rosa_samantha Rh6AG255000 Rh6BG258400 Rh6CG257200 Rh6DG249400
rosa_wichuraiana Rw6G022130

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 495
AciI CCGC 6 cut(s) 75, 155, 257, 338, 347, 350
AclI AACGTT 1 cut(s) 869
AcoI YGGCCR 3 cut(s) 49, 124, 426
AcuI CTGAAG 1 cut(s) 588
AfaI GTAC 2 cut(s) 115, 505
AfiI CCNNNNNNNGG 3 cut(s) 133, 185, 334
AgsI TTSAA 2 cut(s) 388, 808
AjnI CCWGG 2 cut(s) 327, 462
AjuI GAANNNNNNNTTGG 6 cut(s) 368, 400, 780, 812, 882, 914
AleI CACNNNNGTG 1 cut(s) 533
AluBI AGCT 7 cut(s) 81, 108, 289, 478, 670, 785, 893
AluI AGCT 7 cut(s) 81, 108, 289, 478, 670, 785, 893
Alw26I GTCTC 1 cut(s) 395
AoxI GGCC 8 cut(s) 49, 63, 124, 176, 187, 194, 372, 426
ApeKI GCWGC 4 cut(s) 352, 355, 358, 680
Asp700I GAANNNNTTC 1 cut(s) 479
AspLEI GCGC 2 cut(s) 142, 495
AspS9I GGNCC 2 cut(s) 177, 460
AsuHPI GGTGA 1 cut(s) 532
AsuNHI GCTAGC 1 cut(s) 77
AvaII GGWCC 1 cut(s) 460
AxyI CCTNAGG 1 cut(s) 513
BalI TGGCCA 2 cut(s) 51, 428
BanII GRGCYC 1 cut(s) 444
BbvI GCAGC 4 cut(s) 364, 367, 370, 667
BccI CCATC 6 cut(s) 13, 205, 289, 431, 446, 746
BceAI ACGGC 1 cut(s) 111
BcgI CGANNNNNNTGC 2 cut(s) 855, 889
BciT130I CCWGG 2 cut(s) 329, 464
BcoDI GTCTC 1 cut(s) 395
BfaI CTAG 1 cut(s) 78
BisI GCNGC 6 cut(s) 347, 350, 353, 356, 359, 681
BlsI GCNGC 6 cut(s) 348, 351, 354, 357, 360, 682
Bme1390I CCNGG 2 cut(s) 329, 464
Bme18I GGWCC 1 cut(s) 460
BmgT120I GGNCC 2 cut(s) 177, 460
BmiI GGNNCC 3 cut(s) 162, 333, 345
BmrFI CCNGG 2 cut(s) 329, 464
BmsI GCATC 4 cut(s) 480, 562, 585, 667
BmtI GCTAGC 1 cut(s) 81
BpmI CTGGAG 1 cut(s) 692
BpuEI CTTGAG 3 cut(s) 202, 275, 302
BsaJI CCNNGG 1 cut(s) 327
BsaWI WCCGGW 2 cut(s) 110, 364
BsaXI ACNNNNNCTCC 2 cut(s) 11, 41
Bsc4I CCNNNNNNNGG 3 cut(s) 133, 185, 334
Bse118I RCCGGY 2 cut(s) 334, 710
Bse1I ACTGG 1 cut(s) 878
Bse21I CCTNAGG 1 cut(s) 513
Bse3DI GCAATG 3 cut(s) 260, 477, 610
BseBI CCWGG 2 cut(s) 329, 464
BseDI CCNNGG 1 cut(s) 327
BseGI GGATG 1 cut(s) 682
BseLI CCNNNNNNNGG 3 cut(s) 133, 185, 334
BseMI GCAATG 3 cut(s) 260, 477, 610
BseMII CTCAG 2 cut(s) 74, 504
BseNI ACTGG 1 cut(s) 878
BseRI GAGGAG 4 cut(s) 250, 253, 356, 383
BseXI GCAGC 4 cut(s) 364, 367, 370, 667
BsgI GTGCAG 1 cut(s) 665
Bsh1236I CGCG 1 cut(s) 495
BshFI GGCC 8 cut(s) 51, 65, 126, 178, 189, 196, 374, 428
BsiSI CCGG 5 cut(s) 111, 158, 335, 365, 711
BslI CCNNNNNNNGG 3 cut(s) 133, 185, 334
BsmAI GTCTC 1 cut(s) 395
BsmBI CGTCTC 1 cut(s) 395
BsmI GAATGC 1 cut(s) 625
BsnI GGCC 8 cut(s) 51, 65, 126, 178, 189, 196, 374, 428
Bsp1286I GDGCHC 1 cut(s) 444
Bsp143I GATC 1 cut(s) 118
BspACI CCGC 6 cut(s) 75, 155, 257, 338, 347, 350
BspANI GGCC 8 cut(s) 51, 65, 126, 178, 189, 196, 374, 428
BspCNI CTCAG 2 cut(s) 73, 505
BspFNI CGCG 1 cut(s) 495
BspLI GGNNCC 3 cut(s) 162, 333, 345
BspOI GCTAGC 1 cut(s) 81
BsrDI GCAATG 3 cut(s) 260, 477, 610
BsrFI RCCGGY 2 cut(s) 334, 710
BsrI ACTGG 1 cut(s) 878
BssAI RCCGGY 2 cut(s) 334, 710
BssECI CCNNGG 1 cut(s) 327
BssMI GATC 1 cut(s) 118
Bst2UI CCWGG 2 cut(s) 329, 464
Bst4CI ACNGT 1 cut(s) 217
Bst6I CTCTTC 1 cut(s) 308
BstC8I GCNNGC 1 cut(s) 79
BstDEI CTNAG 3 cut(s) 60, 513, 576
BstF5I GGATG 1 cut(s) 682
BstFNI CGCG 1 cut(s) 495
BstHHI GCGC 2 cut(s) 142, 495
BstKTI GATC 1 cut(s) 121
BstMAI GTCTC 1 cut(s) 395
BstMBI GATC 1 cut(s) 118
BstMWI GCNNNNNNNGC 4 cut(s) 352, 355, 358, 439
BstNI CCWGG 2 cut(s) 329, 464
BstSCI CCNGG 2 cut(s) 327, 462
BstUI CGCG 1 cut(s) 495
BstV1I GCAGC 4 cut(s) 364, 367, 370, 667
BstXI CCANNNNNNTGG 2 cut(s) 424, 885
Bsu36I CCTNAGG 1 cut(s) 513
BsuRI GGCC 8 cut(s) 51, 65, 126, 178, 189, 196, 374, 428
BtsCI GGATG 1 cut(s) 682
BtsI GCAGTG 2 cut(s) 429, 528
BtsIMutI CAGTG 4 cut(s) 213, 429, 528, 885
Cac8I GCNNGC 1 cut(s) 79
CfoI GCGC 2 cut(s) 142, 495
Cfr10I RCCGGY 2 cut(s) 334, 710
Cfr13I GGNCC 2 cut(s) 177, 460
Csp6I GTAC 2 cut(s) 114, 504
CspCI CAANNNNNGTGG 2 cut(s) 751, 786
CviAII CATG 1 cut(s) 430
CviQI GTAC 2 cut(s) 114, 504
DdeI CTNAG 3 cut(s) 60, 513, 576
DpnI GATC 1 cut(s) 120
DpnII GATC 1 cut(s) 118
EaeI YGGCCR 3 cut(s) 49, 124, 426
Eam1104I CTCTTC 1 cut(s) 308
EarI CTCTTC 1 cut(s) 308
EciI GGCGGA 2 cut(s) 246, 353
Eco147I AGGCCT 1 cut(s) 65
Eco24I GRGCYC 1 cut(s) 444
Eco47I GGWCC 1 cut(s) 460
Eco57I CTGAAG 1 cut(s) 588
Eco81I CCTNAGG 1 cut(s) 513
EcoO109I RGGNCCY 1 cut(s) 177
EcoRII CCWGG 2 cut(s) 327, 462
EcoT38I GRGCYC 1 cut(s) 444
Esp3I CGTCTC 1 cut(s) 395
FaeI CATG 1 cut(s) 433
FaiI YATR 6 cut(s) 431, 526, 686, 760, 822, 824
FalI AAGNNNNNCTT 2 cut(s) 8, 40
FatI CATG 1 cut(s) 429
Fnu4HI GCNGC 6 cut(s) 347, 350, 353, 356, 359, 681
FokI GGATG 1 cut(s) 689
FriOI GRGCYC 1 cut(s) 444
Fsp4HI GCNGC 6 cut(s) 347, 350, 353, 356, 359, 681
FspBI CTAG 1 cut(s) 78
GlaI GCGC 2 cut(s) 141, 494
GluI GCNGC 6 cut(s) 347, 350, 353, 356, 359, 681
GsuI CTGGAG 1 cut(s) 692
HaeIII GGCC 8 cut(s) 51, 65, 126, 178, 189, 196, 374, 428
HapII CCGG 5 cut(s) 111, 158, 335, 365, 711
HhaI GCGC 2 cut(s) 142, 495
Hin1II CATG 1 cut(s) 433
Hin6I GCGC 2 cut(s) 140, 493
HinP1I GCGC 2 cut(s) 140, 493
HindIII AAGCTT 1 cut(s) 476
HinfI GANTC 2 cut(s) 202, 412
HpaII CCGG 5 cut(s) 111, 158, 335, 365, 711
HphI GGTGA 1 cut(s) 532
Hpy166II GTNNAC 1 cut(s) 12
Hpy188I TCNGA 2 cut(s) 411, 618
Hpy188III TCNNGA 6 cut(s) 281, 292, 302, 388, 512, 589
Hpy8I GTNNAC 1 cut(s) 12
Hpy99I CGWCG 1 cut(s) 102
HpyAV CCTTC 6 cut(s) 219, 391, 536, 773, 808, 829
HpyCH4III ACNGT 1 cut(s) 217
HpyCH4IV ACGT 2 cut(s) 97, 869
HpyCH4V TGCA 7 cut(s) 265, 422, 447, 552, 598, 646, 762
HpyF10VI GCNNNNNNNGC 4 cut(s) 352, 355, 358, 439
HpyF3I CTNAG 3 cut(s) 60, 513, 576
HpySE526I ACGT 2 cut(s) 97, 869
Hsp92II CATG 1 cut(s) 433
HspAI GCGC 2 cut(s) 140, 493
Kzo9I GATC 1 cut(s) 118
LmnI GCTCC 4 cut(s) 113, 160, 343, 782
Lsp1109I GCAGC 4 cut(s) 364, 367, 370, 667
LweI GCATC 4 cut(s) 480, 562, 585, 667
MaeI CTAG 1 cut(s) 78
MaeII ACGT 2 cut(s) 97, 869
MaeIII GTNAC 3 cut(s) 629, 713, 727
MalI GATC 1 cut(s) 120
MboI GATC 1 cut(s) 118
MboII GAAGA 6 cut(s) 16, 325, 403, 581, 604, 916
MhlI GDGCHC 1 cut(s) 444
MlsI TGGCCA 2 cut(s) 51, 428
MluCI AATT 1 cut(s) 33
MluNI TGGCCA 2 cut(s) 51, 428
MmeI TCCRAC 2 cut(s) 63, 684
Mox20I TGGCCA 2 cut(s) 51, 428
MroXI GAANNNNTTC 1 cut(s) 479
MscI TGGCCA 2 cut(s) 51, 428
MseI TTAA 2 cut(s) 90, 828
MslI CAYNNNNRTG 1 cut(s) 533
Msp20I TGGCCA 2 cut(s) 51, 428
MspI CCGG 5 cut(s) 111, 158, 335, 365, 711
MspR9I CCNGG 2 cut(s) 329, 464
Mva1269I GAATGC 1 cut(s) 625
MvaI CCWGG 2 cut(s) 329, 464
MvnI CGCG 1 cut(s) 495
MwoI GCNNNNNNNGC 4 cut(s) 352, 355, 358, 439
NdeII GATC 1 cut(s) 118
NheI GCTAGC 1 cut(s) 77
NlaIII CATG 1 cut(s) 433
NlaIV GGNNCC 3 cut(s) 162, 333, 345
NmuCI GTSAC 2 cut(s) 713, 727
OliI CACNNNNGTG 1 cut(s) 533
PceI AGGCCT 1 cut(s) 65
PctI GAATGC 1 cut(s) 625
PdmI GAANNNNTTC 1 cut(s) 479
PfeI GAWTC 2 cut(s) 202, 412
PfoI TCCNGGA 1 cut(s) 462
PkrI GCNGC 6 cut(s) 348, 351, 354, 357, 360, 682
Psp1406I AACGTT 1 cut(s) 869
Psp6I CCWGG 2 cut(s) 327, 462
PspGI CCWGG 2 cut(s) 327, 462
PspN4I GGNNCC 3 cut(s) 162, 333, 345
PspPI GGNCC 2 cut(s) 177, 460
RsaI GTAC 2 cut(s) 115, 505
RsaNI GTAC 2 cut(s) 114, 504
RseI CAYNNNNRTG 1 cut(s) 533
SaqAI TTAA 2 cut(s) 90, 828
SatI GCNGC 6 cut(s) 347, 350, 353, 356, 359, 681
Sau3AI GATC 1 cut(s) 118
Sau96I GGNCC 2 cut(s) 177, 460
ScrFI CCNGG 2 cut(s) 329, 464
SduI GDGCHC 1 cut(s) 444
SfaNI GCATC 4 cut(s) 480, 562, 585, 667
SinI GGWCC 1 cut(s) 460
SmiMI CAYNNNNRTG 1 cut(s) 533
SmlI CTYRAG 3 cut(s) 181, 281, 290
SmoI CTYRAG 3 cut(s) 181, 281, 290
Sse9I AATT 1 cut(s) 33
SseBI AGGCCT 1 cut(s) 65
SsiI CCGC 6 cut(s) 75, 155, 257, 338, 347, 350
SspMI CTAG 1 cut(s) 78
StuI AGGCCT 1 cut(s) 65
StyD4I CCNGG 2 cut(s) 327, 462
TaaI ACNGT 1 cut(s) 217
TaiI ACGT 2 cut(s) 100, 872
TaqI TCGA 3 cut(s) 200, 311, 488
TasI AATT 1 cut(s) 33
TauI GCSGC 2 cut(s) 349, 352
TfiI GAWTC 2 cut(s) 202, 412
Tru1I TTAA 2 cut(s) 90, 828
Tru9I TTAA 2 cut(s) 90, 828
TscAI CASTG 4 cut(s) 220, 429, 535, 885
TseFI GTSAC 2 cut(s) 713, 727
TseI GCWGC 4 cut(s) 352, 355, 358, 680
Tsp45I GTSAC 2 cut(s) 713, 727
TspDTI ATGAA 3 cut(s) 45, 410, 488
TspRI CASTG 4 cut(s) 220, 429, 535, 885
VpaK11BI GGWCC 1 cut(s) 460
XmnI GAANNNNTTC 1 cut(s) 479
XspI CTAG 1 cut(s) 78
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.