pycom10g13640

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0010525
Physical Location & Seq
Forward (+)
17123783 .. 17124628
846 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 618 bp
ATGGATCCTCCACATGGAAGCAGGCCATGGAGGCCATGCTTACTCCACCATCCCCACCACCACCTCCACCATCATCACCACCACCACCCCTTATTCCTCTGCCCTCTCCATCACCGCCACCACCACCAGGTCCACCACTTCCACCATCTCCGCACCAATTTTGCGTCTTTTCCGCAGAACTCAGAACCCCCAGCTGCCGCCGCGACTCCTTTCCCAACCCATTTGAATTCAGAACCTTCTGAGCTCAAAGAAGAGTACTACGACGATCCTACCGCTCAGACACTGCCGGAGTACGGTGAGATCGATGATGGTGGGTTGGAAGAGGAAGAGGATGACGAGCCGGTTTTCGTCTTAACAGACGAGTGGAAGGAGTTTTTTGCAAAATCTGAAGCTAAGAGAAAACTGGGTACTCTCTTTTCTCTCTCTCTCTCTGCTCTCTACCCTCTCACCCTATTTTTGGAAAATTATTTCCGCGCAACCTTTTCTTGTATGCGGAAATCACTTCCCTTGCATCTCGAGAACACCGACTTCTATGATATGACTCTGTGCTTGATGCGTGTAATTACAGAGAAGCAGCAGGCCAAGAGGAAGGGAAAAGCTAGACAGAGTCAAGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

206

Amino Acids

24.08

Weight (kDa)

6.22

Isoelectric Point (pI)

55.25

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 275
AccII CGCG 2 cut(s) 203, 474
AciI CCGC 8 cut(s) 115, 151, 173, 198, 201, 273, 472, 493
AclWI GGATC 2 cut(s) 12, 260
AcsI RAATTY 1 cut(s) 226
AcuI CTGAAG 1 cut(s) 408
AfaI GTAC 3 cut(s) 257, 293, 409
AfiI CCNNNNNNNGG 4 cut(s) 14, 127, 293, 457
AgsI TTSAA 1 cut(s) 226
AjnI CCWGG 1 cut(s) 126
AluBI AGCT 4 cut(s) 194, 244, 392, 599
AluI AGCT 4 cut(s) 194, 244, 392, 599
Alw21I GWGCWC 1 cut(s) 246
AlwI GGATC 2 cut(s) 12, 260
AlwNI CAGNNNCTG 1 cut(s) 283
Ama87I CYCGRG 1 cut(s) 515
AoxI GGCC 3 cut(s) 23, 32, 579
ApeKI GCWGC 2 cut(s) 194, 574
ApoI RAATTY 1 cut(s) 226
AspLEI GCGC 1 cut(s) 476
AspS9I GGNCC 1 cut(s) 130
AsuHPI GGTGA 4 cut(s) 68, 104, 308, 439
AvaI CYCGRG 1 cut(s) 515
AvaII GGWCC 1 cut(s) 130
BamHI GGATCC 1 cut(s) 4
BanII GRGCYC 1 cut(s) 246
Bbv12I GWGCWC 1 cut(s) 246
BbvI GCAGC 2 cut(s) 181, 586
BccI CCATC 5 cut(s) 57, 78, 117, 153, 302
BciT130I CCWGG 1 cut(s) 128
BfaI CTAG 1 cut(s) 600
BglI GCCNNNNNGGC 1 cut(s) 31
BisI GCNGC 4 cut(s) 195, 198, 201, 575
BlsI GCNGC 4 cut(s) 196, 199, 202, 576
BmcAI AGTACT 1 cut(s) 257
Bme1390I CCNGG 1 cut(s) 128
Bme18I GGWCC 1 cut(s) 130
BmeT110I CYCGRG 1 cut(s) 515
BmgT120I GGNCC 1 cut(s) 130
BmiI GGNNCC 1 cut(s) 6
BmrFI CCNGG 1 cut(s) 128
BmrI ACTGGG 1 cut(s) 413
BmsI GCATC 2 cut(s) 520, 543
BmuI ACTGGG 1 cut(s) 413
Bsa29I ATCGAT 1 cut(s) 303
BsaJI CCNNGG 1 cut(s) 26
Bsc4I CCNNNNNNNGG 4 cut(s) 14, 127, 293, 457
Bse118I RCCGGY 1 cut(s) 340
Bse1I ACTGG 1 cut(s) 408
BseBI CCWGG 1 cut(s) 128
BseCI ATCGAT 1 cut(s) 303
BseDI CCNNGG 1 cut(s) 26
BseGI GGATG 2 cut(s) 49, 337
BseLI CCNNNNNNNGG 4 cut(s) 14, 127, 293, 457
BseMII CTCAG 3 cut(s) 195, 231, 290
BseNI ACTGG 1 cut(s) 408
BseXI GCAGC 2 cut(s) 181, 586
BseYI CCCAGC 1 cut(s) 190
Bsh1236I CGCG 2 cut(s) 203, 474
BshFI GGCC 3 cut(s) 25, 34, 581
BshVI ATCGAT 1 cut(s) 303
BsiHKAI GWGCWC 1 cut(s) 246
BsiHKCI CYCGRG 1 cut(s) 515
BsiSI CCGG 2 cut(s) 287, 341
BslI CCNNNNNNNGG 4 cut(s) 14, 127, 293, 457
BsnI GGCC 3 cut(s) 25, 34, 581
BsoBI CYCGRG 1 cut(s) 515
Bsp1286I GDGCHC 1 cut(s) 246
Bsp143I GATC 3 cut(s) 4, 265, 300
Bsp19I CCATGG 1 cut(s) 26
BspACI CCGC 8 cut(s) 115, 151, 173, 198, 201, 273, 472, 493
BspANI GGCC 3 cut(s) 25, 34, 581
BspCNI CTCAG 3 cut(s) 194, 232, 289
BspDI ATCGAT 1 cut(s) 303
BspFNI CGCG 2 cut(s) 203, 474
BspLI GGNNCC 1 cut(s) 6
BspPI GGATC 2 cut(s) 12, 260
BsrBI CCGCTC 1 cut(s) 275
BsrFI RCCGGY 1 cut(s) 340
BsrI ACTGG 1 cut(s) 408
BssAI RCCGGY 1 cut(s) 340
BssECI CCNNGG 1 cut(s) 26
BssMI GATC 3 cut(s) 4, 265, 300
BssT1I CCWWGG 1 cut(s) 26
Bst2UI CCWGG 1 cut(s) 128
Bst4CI ACNGT 1 cut(s) 296
Bst6I CTCTTC 3 cut(s) 246, 315, 321
BstC8I GCNNGC 2 cut(s) 23, 579
BstDEI CTNAG 4 cut(s) 181, 240, 276, 393
BstDSI CCRYGG 1 cut(s) 26
BstF5I GGATG 2 cut(s) 49, 337
BstFNI CGCG 2 cut(s) 203, 474
BstHHI GCGC 1 cut(s) 476
BstKTI GATC 3 cut(s) 7, 268, 303
BstMBI GATC 3 cut(s) 4, 265, 300
BstMWI GCNNNNNNNGC 2 cut(s) 31, 200
BstNI CCWGG 1 cut(s) 128
BstSCI CCNGG 1 cut(s) 126
BstUI CGCG 2 cut(s) 203, 474
BstV1I GCAGC 2 cut(s) 181, 586
BstX2I RGATCY 1 cut(s) 4
BstYI RGATCY 1 cut(s) 4
Bsu15I ATCGAT 1 cut(s) 303
BsuRI GGCC 3 cut(s) 25, 34, 581
BsuTUI ATCGAT 1 cut(s) 303
BtgI CCRYGG 1 cut(s) 26
BtsCI GGATG 2 cut(s) 49, 337
BtsI GCAGTG 1 cut(s) 281
BtsIMutI CAGTG 1 cut(s) 281
Cac8I GCNNGC 2 cut(s) 23, 579
CaiI CAGNNNCTG 1 cut(s) 283
CfoI GCGC 1 cut(s) 476
Cfr10I RCCGGY 1 cut(s) 340
Cfr13I GGNCC 1 cut(s) 130
ClaI ATCGAT 1 cut(s) 303
CseI GACGC 1 cut(s) 153
CsiI ACCWGGT 1 cut(s) 126
Csp6I GTAC 3 cut(s) 256, 292, 408
CviAII CATG 3 cut(s) 14, 27, 36
CviJI RGCY 8 cut(s) 25, 34, 194, 244, 340, 392, 581, 599
CviKI_1 RGCY 8 cut(s) 25, 34, 194, 244, 340, 392, 581, 599
CviQI GTAC 3 cut(s) 256, 292, 408
DdeI CTNAG 4 cut(s) 181, 240, 276, 393
DpnI GATC 3 cut(s) 6, 267, 302
DpnII GATC 3 cut(s) 4, 265, 300
Eam1104I CTCTTC 3 cut(s) 246, 315, 321
EarI CTCTTC 3 cut(s) 246, 315, 321
Ecl136II GAGCTC 1 cut(s) 244
Eco130I CCWWGG 1 cut(s) 26
Eco24I GRGCYC 1 cut(s) 246
Eco47I GGWCC 1 cut(s) 130
Eco53kI GAGCTC 1 cut(s) 244
Eco57I CTGAAG 1 cut(s) 408
Eco88I CYCGRG 1 cut(s) 515
EcoICRI GAGCTC 1 cut(s) 244
EcoRI GAATTC 1 cut(s) 226
EcoRII CCWGG 1 cut(s) 126
EcoT14I CCWWGG 1 cut(s) 26
EcoT38I GRGCYC 1 cut(s) 246
ErhI CCWWGG 1 cut(s) 26
FaeI CATG 3 cut(s) 17, 30, 39
FaiI YATR 6 cut(s) 15, 28, 37, 491, 534, 539
FatI CATG 3 cut(s) 13, 26, 35
Fnu4HI GCNGC 4 cut(s) 195, 198, 201, 575
FokI GGATG 2 cut(s) 36, 344
FriOI GRGCYC 1 cut(s) 246
Fsp4HI GCNGC 4 cut(s) 195, 198, 201, 575
FspBI CTAG 1 cut(s) 600
GlaI GCGC 1 cut(s) 475
GluI GCNGC 4 cut(s) 195, 198, 201, 575
GsaI CCCAGC 1 cut(s) 194
HaeIII GGCC 3 cut(s) 25, 34, 581
HapII CCGG 2 cut(s) 287, 341
HgaI GACGC 1 cut(s) 153
HhaI GCGC 1 cut(s) 476
Hin1II CATG 3 cut(s) 17, 30, 39
Hin6I GCGC 1 cut(s) 474
HinP1I GCGC 1 cut(s) 474
HinfI GANTC 3 cut(s) 205, 541, 607
HpaII CCGG 2 cut(s) 287, 341
HphI GGTGA 4 cut(s) 68, 104, 308, 439
Hpy166II GTNNAC 1 cut(s) 133
Hpy188I TCNGA 5 cut(s) 184, 232, 241, 279, 388
Hpy188III TCNNGA 3 cut(s) 515, 517, 611
Hpy8I GTNNAC 1 cut(s) 133
Hpy99I CGWCG 1 cut(s) 266
HpyAV CCTTC 3 cut(s) 246, 361, 583
HpyCH4III ACNGT 1 cut(s) 296
HpyCH4V TGCA 2 cut(s) 380, 511
HpyF10VI GCNNNNNNNGC 2 cut(s) 31, 200
HpyF3I CTNAG 4 cut(s) 181, 240, 276, 393
Hsp92II CATG 3 cut(s) 17, 30, 39
HspAI GCGC 1 cut(s) 474
Kzo9I GATC 3 cut(s) 4, 265, 300
LpnPI CCDG 8 cut(s) 7, 113, 140, 204, 300, 354, 389, 563
Lsp1109I GCAGC 2 cut(s) 181, 586
LweI GCATC 2 cut(s) 520, 543
MabI ACCWGGT 1 cut(s) 126
MaeI CTAG 1 cut(s) 600
MalI GATC 3 cut(s) 6, 267, 302
MbiI CCGCTC 1 cut(s) 275
MboI GATC 3 cut(s) 4, 265, 300
MboII GAAGA 3 cut(s) 263, 332, 338
MflI RGATCY 1 cut(s) 4
MhlI GDGCHC 1 cut(s) 246
MluCI AATT 4 cut(s) 157, 226, 463, 561
MlyI GAGTC 3 cut(s) 199, 535, 616
MmeI TCCRAC 1 cut(s) 297
MnlI CCTC 9 cut(s) 18, 24, 74, 107, 114, 316, 322, 453, 579
MseI TTAA 1 cut(s) 353
MspA1I CMGCKG 1 cut(s) 194
MspI CCGG 2 cut(s) 287, 341
MspR9I CCNGG 1 cut(s) 128
MvaI CCWGG 1 cut(s) 128
MvnI CGCG 2 cut(s) 203, 474
MwoI GCNNNNNNNGC 2 cut(s) 31, 200
NcoI CCATGG 1 cut(s) 26
NdeII GATC 3 cut(s) 4, 265, 300
NlaIII CATG 3 cut(s) 17, 30, 39
NlaIV GGNNCC 1 cut(s) 6
PaeR7I CTCGAG 1 cut(s) 515
PcsI WCGNNNNNNNCGW 2 cut(s) 300, 522
PflFI GACNNNGTC 1 cut(s) 606
PkrI GCNGC 4 cut(s) 196, 199, 202, 576
PleI GAGTC 3 cut(s) 199, 535, 615
PpsI GAGTC 3 cut(s) 199, 535, 615
Psp124BI GAGCTC 1 cut(s) 246
Psp6I CCWGG 1 cut(s) 126
PspFI CCCAGC 1 cut(s) 190
PspGI CCWGG 1 cut(s) 126
PspN4I GGNNCC 1 cut(s) 6
PspPI GGNCC 1 cut(s) 130
PstNI CAGNNNCTG 1 cut(s) 283
PsuI RGATCY 1 cut(s) 4
PsyI GACNNNGTC 1 cut(s) 606
PvuII CAGCTG 1 cut(s) 194
RsaI GTAC 3 cut(s) 257, 293, 409
RsaNI GTAC 3 cut(s) 256, 292, 408
SacI GAGCTC 1 cut(s) 246
SaqAI TTAA 1 cut(s) 353
SatI GCNGC 4 cut(s) 195, 198, 201, 575
Sau3AI GATC 3 cut(s) 4, 265, 300
Sau96I GGNCC 1 cut(s) 130
ScaI AGTACT 1 cut(s) 257
SchI GAGTC 3 cut(s) 199, 535, 616
ScrFI CCNGG 1 cut(s) 128
SduI GDGCHC 1 cut(s) 246
SetI ASST 8 cut(s) 66, 132, 196, 238, 246, 394, 482, 601
SexAI ACCWGGT 1 cut(s) 126
SfaNI GCATC 2 cut(s) 520, 543
SfiI GGCCNNNNNGGCC 1 cut(s) 31
Sfr274I CTCGAG 1 cut(s) 515
SinI GGWCC 1 cut(s) 130
SlaI CTCGAG 1 cut(s) 515
SmlI CTYRAG 1 cut(s) 515
SmoI CTYRAG 1 cut(s) 515
Sse9I AATT 4 cut(s) 157, 226, 463, 561
SsiI CCGC 8 cut(s) 115, 151, 173, 198, 201, 273, 472, 493
SspMI CTAG 1 cut(s) 600
SstI GAGCTC 1 cut(s) 246
StyD4I CCNGG 1 cut(s) 126
StyI CCWWGG 1 cut(s) 26
TaaI ACNGT 1 cut(s) 296
TaqI TCGA 2 cut(s) 303, 516
TasI AATT 4 cut(s) 157, 226, 463, 561
TatI WGTACW 1 cut(s) 255
TauI GCSGC 2 cut(s) 200, 203
Tru1I TTAA 1 cut(s) 353
Tru9I TTAA 1 cut(s) 353
TscAI CASTG 1 cut(s) 288
TseI GCWGC 2 cut(s) 194, 574
TspRI CASTG 1 cut(s) 288
Tth111I GACNNNGTC 1 cut(s) 606
VpaK11BI GGWCC 1 cut(s) 130
XapI RAATTY 1 cut(s) 226
XhoI CTCGAG 1 cut(s) 515
XspI CTAG 1 cut(s) 600
ZrmI AGTACT 1 cut(s) 257
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.