pycom10g14750

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0012747
Physical Location & Seq
Reverse (-)
18146769 .. 18149597
2829 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1107 bp
ATGTCAGGTACAACTCCCAGCTGCAAGGTCTTTGCTGCCGTCGCTCTGATAGTGGCAATGGCGGCAATTGCACCTCAAGCCGAGGCAGCTCGTGCTTTCTTTGTGTTTGGGGACTCACTCGTCGACAGTGGCAACAACAACTACTTAGCGACCACTGCACGTGCCGACTCTCCTCCCTACGGCATTGATTTTCCGACTCACCGCCCCACCGGCCGCTTCTCCAATGGCTTAAACCTCCCCGACATTATCAGTGAGCAAATTGGATCTGAACGCGTGTTGCCCTATTTGAGTAGTGAGCTCATAGGAGAAAAGCTGCTTATTGGAGCCAACTTTGCTTCTGCAGGAATTGGAATCCTCAACGATACCGGGGTTCAATTTATTACCATAATAAGGATCTCCAGGCAGTTTGAATATTTCCAACAATACCAGCAACGGCTGACTGCACTTGTTGGAGCCGAGCAGGCAACACGGCTGGTGAACAATGCACTTGTGCTCATAACGCTTGGTGGCAATGACTTCGTCAACAACTATTTCCTCCAACCTTTCTCTTTACGATCACGGCAATACTCCATCCCCGATTTTTCCCGTTACCTCATCTCCGAGTACCGAAAAATTCTCCTGAGATTATACGAATTAGGAGCCCGGAGGGTATTGGTGACTGGCACAGGCCCATTGGGCTGTGTTCCATCTGAGCTAGCCCAGAGGAGTCAAAATGGTGAGTGCAACCCAGAACTACAAAATGCAGGAGCAATCTTCAACTCACAACTCAACCAGATGGCTCAAGGACTCAACAAGCAGCTCGGTTCAGATATTTTTATCACTGCTAATGCATTTGATATGCACACGGACTTCCTCTCCAACCCCCAAAAATTTGGTTTTGTGACATCAAAAGTGGCATGTTGTGGGCAAGGACCCTATAATGGGTTGGGACAGTGCAATCCATTGTCAAACCTGTGCCCAAACAGGGATCTCTATGCTTTCTGGGATGCTTTCCATCCAACTGAGAAAGCAACCAGGCTAATTGTGCAGCAGATTATGTCCGGGTCTAGCAAGTACATGAACCCAATGAACCTAAGCACCATAATGGCCTTGGACTCCATGACCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

369

Amino Acids

40.28

Weight (kDa)

6.82

Isoelectric Point (pI)

38.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 34 - 344 1.2e-30 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012747)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G28780
fragaria_vesca FvH4_2g19880
malus_domestica MD05G1183000.v1.1 MD10G1171100.v1.1
prunus_persica Prupe.8G209600_v2.0.a1
pyrus_communis pycom10g14750
rosa_chinensis RchiOBHm_Chr6g0285571
rosa_laevigata RLG00000012626
rosa_multiflora Rmu_co8252409.1_g000001 Rmu_sc0017770.1_g000005
rosa_roxburghii Rroxscaffold_7G00182950
rosa_rugosa Rorug06G0175600 Rorug06G0175700.1
rosa_samantha Rh6AG287200 Rh6BG288400 Rh6CG289600 Rh6DG282400
rosa_wichuraiana Rw6G024690

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 119
AccI GTMKAC 1 cut(s) 123
AccII CGCG 1 cut(s) 273
AciI CCGC 3 cut(s) 62, 202, 214
AclWI GGATC 3 cut(s) 271, 401, 975
AcoI YGGCCR 1 cut(s) 211
AcsI RAATTY 2 cut(s) 612, 869
AcvI CACGTG 1 cut(s) 161
AfaI GTAC 3 cut(s) 10, 605, 1055
AfiI CCNNNNNNNGG 5 cut(s) 179, 390, 920, 921, 964
AflIII ACRYGT 1 cut(s) 271
AgsI TTSAA 3 cut(s) 374, 410, 757
AjnI CCWGG 2 cut(s) 398, 1013
AluBI AGCT 6 cut(s) 21, 89, 298, 313, 694, 799
AluI AGCT 6 cut(s) 21, 89, 298, 313, 694, 799
Alw21I GWGCWC 2 cut(s) 300, 495
AlwI GGATC 3 cut(s) 271, 401, 975
AoxI GGCC 3 cut(s) 211, 667, 1086
ApeKI GCWGC 6 cut(s) 21, 35, 86, 313, 796, 1027
ApoI RAATTY 2 cut(s) 612, 869
AspS9I GGNCC 2 cut(s) 668, 911
AsuC2I CCSGG 3 cut(s) 367, 643, 1042
AsuHPI GGTGA 4 cut(s) 191, 487, 667, 728
AsuNHI GCTAGC 1 cut(s) 694
AvaII GGWCC 1 cut(s) 911
BaeGI GKGCMC 1 cut(s) 959
BaeI ACNNNNGTAYC 2 cut(s) 354, 387
BanII GRGCYC 2 cut(s) 300, 643
BauI CACGAG 1 cut(s) 90
BbrPI CACGTG 1 cut(s) 161
Bbv12I GWGCWC 2 cut(s) 300, 495
BbvI GCAGC 6 cut(s) 8, 22, 98, 300, 808, 1039
BccI CCATC 4 cut(s) 578, 694, 769, 1002
BceAI ACGGC 5 cut(s) 23, 196, 449, 485, 575
BcgI CGANNNNNNTGC 2 cut(s) 499, 533
BciT130I CCWGG 2 cut(s) 400, 1015
BcnI CCSGG 3 cut(s) 367, 643, 1042
BfaI CTAG 2 cut(s) 695, 1047
BfmI CTRYAG 1 cut(s) 339
BglI GCCNNNNNGGC 3 cut(s) 210, 461, 675
BisI GCNGC 8 cut(s) 22, 36, 63, 87, 214, 314, 797, 1028
BlsI GCNGC 8 cut(s) 23, 37, 64, 88, 215, 315, 798, 1029
Bme1390I CCNGG 5 cut(s) 367, 400, 643, 1015, 1042
Bme18I GGWCC 1 cut(s) 911
BmgT120I GGNCC 2 cut(s) 668, 911
BmiI GGNNCC 4 cut(s) 325, 454, 640, 913
BmrFI CCNGG 5 cut(s) 367, 400, 643, 1015, 1042
BmsI GCATC 1 cut(s) 976
BmtI GCTAGC 1 cut(s) 698
BpmI CTGGAG 1 cut(s) 382
Bpu10I CCTNAGC 1 cut(s) 1073
BpuEI CTTGAG 2 cut(s) 60, 765
BpuMI CCSGG 3 cut(s) 367, 643, 1042
BsaAI YACGTR 1 cut(s) 161
BsaJI CCNNGG 3 cut(s) 81, 366, 1089
BsaXI ACNNNNNCTCC 4 cut(s) 581, 611, 839, 869
Bsc4I CCNNNNNNNGG 5 cut(s) 179, 390, 920, 921, 964
Bse118I RCCGGY 1 cut(s) 209
Bse1I ACTGG 1 cut(s) 664
Bse3DI GCAATG 2 cut(s) 63, 517
BseBI CCWGG 2 cut(s) 400, 1015
BseDI CCNNGG 3 cut(s) 81, 366, 1089
BseGI GGATG 3 cut(s) 570, 991, 994
BseLI CCNNNNNNNGG 5 cut(s) 179, 390, 920, 921, 964
BseMI GCAATG 2 cut(s) 63, 517
BseMII CTCAG 3 cut(s) 611, 681, 993
BseNI ACTGG 1 cut(s) 664
BseRI GAGGAG 2 cut(s) 162, 718
BseSI GKGCMC 1 cut(s) 959
BseX3I CGGCCG 1 cut(s) 211
BseXI GCAGC 6 cut(s) 8, 22, 98, 300, 808, 1039
BseYI CCCAGC 1 cut(s) 17
BsgI GTGCAG 3 cut(s) 141, 426, 1046
Bsh1236I CGCG 1 cut(s) 273
Bsh1285I CGRYCG 1 cut(s) 214
BshFI GGCC 3 cut(s) 213, 669, 1088
BsiEI CGRYCG 1 cut(s) 214
BsiHKAI GWGCWC 2 cut(s) 300, 495
BsiSI CCGG 4 cut(s) 210, 366, 643, 1041
BslFI GGGAC 2 cut(s) 125, 942
BslI CCNNNNNNNGG 5 cut(s) 179, 390, 920, 921, 964
BsmFI GGGAC 2 cut(s) 125, 942
BsnI GGCC 3 cut(s) 213, 669, 1088
Bsp1286I GDGCHC 4 cut(s) 300, 495, 643, 959
Bsp143I GATC 4 cut(s) 263, 393, 554, 967
BspACI CCGC 3 cut(s) 62, 202, 214
BspANI GGCC 3 cut(s) 213, 669, 1088
BspCNI CTCAG 3 cut(s) 612, 682, 994
BspFNI CGCG 1 cut(s) 273
BspLI GGNNCC 4 cut(s) 325, 454, 640, 913
BspMAI CTGCAG 1 cut(s) 343
BspOI GCTAGC 1 cut(s) 698
BspPI GGATC 3 cut(s) 271, 401, 975
BsrDI GCAATG 2 cut(s) 63, 517
BsrFI RCCGGY 1 cut(s) 209
BsrI ACTGG 1 cut(s) 664
BssAI RCCGGY 1 cut(s) 209
BssECI CCNNGG 3 cut(s) 81, 366, 1089
BssMI GATC 4 cut(s) 263, 393, 554, 967
BssSI CACGAG 1 cut(s) 90
BssT1I CCWWGG 1 cut(s) 1089
Bst2BI CACGAG 1 cut(s) 90
Bst2UI CCWGG 2 cut(s) 400, 1015
Bst4CI ACNGT 2 cut(s) 128, 933
BstAPI GCANNNNNTGC 1 cut(s) 92
BstBAI YACGTR 1 cut(s) 161
BstC8I GCNNGC 2 cut(s) 462, 696
BstDEI CTNAG 5 cut(s) 145, 620, 690, 1002, 1073
BstF5I GGATG 3 cut(s) 570, 991, 994
BstFNI CGCG 1 cut(s) 273
BstKTI GATC 4 cut(s) 266, 396, 557, 970
BstMBI GATC 4 cut(s) 263, 393, 554, 967
BstMCI CGRYCG 1 cut(s) 214
BstNI CCWGG 2 cut(s) 400, 1015
BstNSI RCATGY 1 cut(s) 900
BstSCI CCNGG 5 cut(s) 365, 398, 641, 1013, 1040
BstSFI CTRYAG 1 cut(s) 339
BstSLI GKGCMC 1 cut(s) 959
BstUI CGCG 1 cut(s) 273
BstV1I GCAGC 6 cut(s) 8, 22, 98, 300, 808, 1039
BstX2I RGATCY 3 cut(s) 263, 393, 967
BstXI CCANNNNNNTGG 1 cut(s) 872
BstYI RGATCY 3 cut(s) 263, 393, 967
BstZI CGGCCG 1 cut(s) 211
BsuRI GGCC 3 cut(s) 213, 669, 1088
BtsCI GGATG 3 cut(s) 570, 991, 994
BtsI GCAGTG 2 cut(s) 153, 819
BtsIMutI CAGTG 5 cut(s) 133, 153, 256, 819, 938
Cac8I GCNNGC 2 cut(s) 462, 696
Cfr10I RCCGGY 1 cut(s) 209
Cfr13I GGNCC 2 cut(s) 668, 911
Csp6I GTAC 3 cut(s) 9, 604, 1054
CviAII CATG 3 cut(s) 897, 1057, 1099
CviQI GTAC 3 cut(s) 9, 604, 1054
DdeI CTNAG 5 cut(s) 145, 620, 690, 1002, 1073
DpnI GATC 4 cut(s) 265, 395, 556, 969
DpnII GATC 4 cut(s) 263, 393, 554, 967
DrdI GACNNNNNNGTC 1 cut(s) 119
DseDI GACNNNNNNGTC 1 cut(s) 119
EaeI YGGCCR 1 cut(s) 211
EagI CGGCCG 1 cut(s) 211
Ecl136II GAGCTC 1 cut(s) 298
EclXI CGGCCG 1 cut(s) 211
Eco130I CCWWGG 1 cut(s) 1089
Eco24I GRGCYC 2 cut(s) 300, 643
Eco47I GGWCC 1 cut(s) 911
Eco52I CGGCCG 1 cut(s) 211
Eco53kI GAGCTC 1 cut(s) 298
Eco72I CACGTG 1 cut(s) 161
EcoICRI GAGCTC 1 cut(s) 298
EcoO109I RGGNCCY 1 cut(s) 911
EcoRII CCWGG 2 cut(s) 398, 1013
EcoT14I CCWWGG 1 cut(s) 1089
EcoT22I ATGCAT 1 cut(s) 832
EcoT38I GRGCYC 2 cut(s) 300, 643
ErhI CCWWGG 1 cut(s) 1089
FaeI CATG 3 cut(s) 900, 1060, 1102
FaqI GGGAC 2 cut(s) 125, 942
FatI CATG 3 cut(s) 896, 1056, 1098
FblI GTMKAC 1 cut(s) 123
Fnu4HI GCNGC 8 cut(s) 22, 36, 63, 87, 214, 314, 797, 1028
FokI GGATG 3 cut(s) 557, 981, 998
FriOI GRGCYC 2 cut(s) 300, 643
Fsp4HI GCNGC 8 cut(s) 22, 36, 63, 87, 214, 314, 797, 1028
FspBI CTAG 2 cut(s) 695, 1047
GluI GCNGC 8 cut(s) 22, 36, 63, 87, 214, 314, 797, 1028
GsaI CCCAGC 1 cut(s) 21
GsuI CTGGAG 1 cut(s) 382
HaeIII GGCC 3 cut(s) 213, 669, 1088
HapII CCGG 4 cut(s) 210, 366, 643, 1041
Hin1II CATG 3 cut(s) 900, 1060, 1102
HincII GTYRAC 2 cut(s) 124, 523
HindII GTYRAC 2 cut(s) 124, 523
HinfI GANTC 7 cut(s) 113, 167, 196, 351, 706, 786, 1094
HpaII CCGG 4 cut(s) 210, 366, 643, 1041
HphI GGTGA 4 cut(s) 191, 487, 667, 728
Hpy166II GTNNAC 3 cut(s) 124, 478, 523
Hpy188I TCNGA 6 cut(s) 48, 195, 268, 601, 691, 808
Hpy188III TCNNGA 1 cut(s) 619
Hpy8I GTNNAC 3 cut(s) 124, 478, 523
Hpy99I CGWCG 2 cut(s) 44, 125
HpyCH4III ACNGT 2 cut(s) 128, 933
HpyCH4IV ACGT 1 cut(s) 160
HpyF3I CTNAG 5 cut(s) 145, 620, 690, 1002, 1073
HpySE526I ACGT 1 cut(s) 160
Hsp92II CATG 3 cut(s) 900, 1060, 1102
Kzo9I GATC 4 cut(s) 263, 393, 554, 967
LmnI GCTCC 4 cut(s) 323, 452, 638, 746
Lsp1109I GCAGC 6 cut(s) 8, 22, 98, 300, 808, 1039
LweI GCATC 1 cut(s) 976
MaeI CTAG 2 cut(s) 695, 1047
MaeII ACGT 1 cut(s) 160
MaeIII GTNAC 3 cut(s) 587, 655, 880
MalI GATC 4 cut(s) 265, 395, 556, 969
MboI GATC 4 cut(s) 263, 393, 554, 967
MboII GAAGA 1 cut(s) 745
MfeI CAATTG 1 cut(s) 66
MflI RGATCY 3 cut(s) 263, 393, 967
MhlI GDGCHC 4 cut(s) 300, 495, 643, 959
MluCI AATT 8 cut(s) 66, 258, 345, 374, 612, 632, 869, 1020
MluI ACGCGT 1 cut(s) 271
MlyI GAGTC 6 cut(s) 107, 161, 190, 715, 780, 1088
MmeI TCCRAC 6 cut(s) 218, 430, 442, 562, 882, 1022
Mph1103I ATGCAT 1 cut(s) 832
MseI TTAA 1 cut(s) 230
MslI CAYNNNNRTG 1 cut(s) 1082
MspA1I CMGCKG 1 cut(s) 21
MspI CCGG 4 cut(s) 210, 366, 643, 1041
MspR9I CCNGG 5 cut(s) 367, 400, 643, 1015, 1042
MunI CAATTG 1 cut(s) 66
MvaI CCWGG 2 cut(s) 400, 1015
MvnI CGCG 1 cut(s) 273
NciI CCSGG 3 cut(s) 367, 643, 1042
NdeII GATC 4 cut(s) 263, 393, 554, 967
NheI GCTAGC 1 cut(s) 694
NlaIII CATG 3 cut(s) 900, 1060, 1102
NlaIV GGNNCC 4 cut(s) 325, 454, 640, 913
NmeAIII GCCGAG 2 cut(s) 106, 481
NmuCI GTSAC 2 cut(s) 655, 880
NsiI ATGCAT 1 cut(s) 832
NspI RCATGY 1 cut(s) 900
PfeI GAWTC 1 cut(s) 351
PflFI GACNNNGTC 1 cut(s) 518
PkrI GCNGC 8 cut(s) 23, 37, 64, 88, 215, 315, 798, 1029
PleI GAGTC 6 cut(s) 107, 161, 190, 714, 780, 1088
PmaCI CACGTG 1 cut(s) 161
PmlI CACGTG 1 cut(s) 161
PpsI GAGTC 6 cut(s) 107, 161, 190, 714, 780, 1088
Ppu21I YACGTR 1 cut(s) 161
PpuMI RGGWCCY 1 cut(s) 911
Psp124BI GAGCTC 1 cut(s) 300
Psp5II RGGWCCY 1 cut(s) 911
Psp6I CCWGG 2 cut(s) 398, 1013
PspCI CACGTG 1 cut(s) 161
PspFI CCCAGC 1 cut(s) 17
PspGI CCWGG 2 cut(s) 398, 1013
PspN4I GGNNCC 4 cut(s) 325, 454, 640, 913
PspPI GGNCC 2 cut(s) 668, 911
PspPPI RGGWCCY 1 cut(s) 911
PstI CTGCAG 1 cut(s) 343
PsuI RGATCY 3 cut(s) 263, 393, 967
PsyI GACNNNGTC 1 cut(s) 518
PvuII CAGCTG 1 cut(s) 21
RsaI GTAC 3 cut(s) 10, 605, 1055
RsaNI GTAC 3 cut(s) 9, 604, 1054
RseI CAYNNNNRTG 1 cut(s) 1082
SacI GAGCTC 1 cut(s) 300
SalI GTCGAC 1 cut(s) 122
SaqAI TTAA 1 cut(s) 230
SatI GCNGC 8 cut(s) 22, 36, 63, 87, 214, 314, 797, 1028
Sau3AI GATC 4 cut(s) 263, 393, 554, 967
Sau96I GGNCC 2 cut(s) 668, 911
SchI GAGTC 6 cut(s) 107, 161, 190, 715, 780, 1088
ScrFI CCNGG 5 cut(s) 367, 400, 643, 1015, 1042
SduI GDGCHC 4 cut(s) 300, 495, 643, 959
SfaNI GCATC 1 cut(s) 976
SfcI CTRYAG 1 cut(s) 339
SinI GGWCC 1 cut(s) 911
SmiMI CAYNNNNRTG 1 cut(s) 1082
SmlI CTYRAG 2 cut(s) 75, 780
SmoI CTYRAG 2 cut(s) 75, 780
Sse9I AATT 8 cut(s) 66, 258, 345, 374, 612, 632, 869, 1020
SsiI CCGC 3 cut(s) 62, 202, 214
SspI AATATT 1 cut(s) 413
SspMI CTAG 2 cut(s) 695, 1047
SstI GAGCTC 1 cut(s) 300
StyD4I CCNGG 5 cut(s) 365, 398, 641, 1013, 1040
StyI CCWWGG 1 cut(s) 1089
TaaI ACNGT 2 cut(s) 128, 933
TaiI ACGT 1 cut(s) 163
TaqI TCGA 1 cut(s) 123
TasI AATT 8 cut(s) 66, 258, 345, 374, 612, 632, 869, 1020
TatI WGTACW 1 cut(s) 1053
TauI GCSGC 2 cut(s) 65, 216
TfiI GAWTC 1 cut(s) 351
Tru1I TTAA 1 cut(s) 230
Tru9I TTAA 1 cut(s) 230
TscAI CASTG 5 cut(s) 133, 160, 256, 826, 938
TseFI GTSAC 2 cut(s) 655, 880
TseI GCWGC 6 cut(s) 21, 35, 86, 313, 796, 1027
Tsp45I GTSAC 2 cut(s) 655, 880
TspDTI ATGAA 2 cut(s) 1073, 1082
TspGWI ACGGA 1 cut(s) 860
TspRI CASTG 5 cut(s) 133, 160, 256, 826, 938
Tth111I GACNNNGTC 1 cut(s) 518
VpaK11BI GGWCC 1 cut(s) 911
XapI RAATTY 2 cut(s) 612, 869
XceI RCATGY 1 cut(s) 900
XcmI CCANNNNNNNNNTGG 1 cut(s) 1087
XmiI GTMKAC 1 cut(s) 123
XspI CTAG 2 cut(s) 695, 1047
Zsp2I ATGCAT 1 cut(s) 832
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.