pycom10g15160

Photosystem II reaction center PSB28 protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0010122
Physical Location & Seq
Reverse (-)
18484487 .. 18485777
1291 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 693 bp
ATGAATCGATATAATTCACGAATCATCAAGTTCTCAGTGAATGGCTGTCCTTGTTACACACCACTCTATCCCCAAACCACAACTCCTCTCTCCTCTTTAACACACACACATATACTCTCCCCCTCTCTCTCTCCACCCTCTACTTCTGCAATGGCAACCCTGCAGTCACTTGCGTTCTCTTCTCCACTCTCACAATGTCTGCAGCAACCACGCCTTCATTCGGGCTTGTTATGTTGGGGTGTTCATAGAAGTGCAGAATCCACATTCAATGGCAAGTCTTTGCGCGTGTCTCGTCCCCAATGGGCGCCATTAAGACGGTGTACTCAAGCATCCAGGTCTATTAAAATGATGGTCAAACCGAAAATTCAGTTCATCCAAGGGACCGATGAACAGACAATACCAGATGTGAGGCTGACCAAATCAAAAGACGGTACAAATGGTGTGGCAATTTTCAAGTTTGAGCAGCCTTCTGTGTTTGACTCCTCTGGTGAGTTTGGTGACATCACTGGCTTTTACATGATTGACGAAGAAGGGGTCCTTTCATCGGTTGATGTTAACGCCAAATTTGTCAACGGAAAGCCTGCAGGAATAGAAGCAAAGTACATAATGCGAACTCCGAGAGATTGGGACAGATTCATGAGATTCATGGAACGCTATGCAAATGACAATGGCTTGAGTTTCATAAAAAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00195 ko01100 map00195 map01100
Pfam Domains
Protein Families
Psb28

Protein Analysis

231

Amino Acids

25.79

Weight (kDa)

9.48

Isoelectric Point (pI)

50.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Psb28 PF03912 122 - 227 2.8e-42 Psb28 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 304
AccII CGCG 1 cut(s) 285
AcsI RAATTY 2 cut(s) 363, 563
AcyI GRCGYC 1 cut(s) 305
AfaI GTAC 3 cut(s) 322, 433, 602
AfiI CCNNNNNNNGG 2 cut(s) 220, 544
AgsI TTSAA 2 cut(s) 268, 454
AjnI CCWGG 1 cut(s) 332
Alw26I GTCTC 1 cut(s) 294
ApeKI GCWGC 2 cut(s) 202, 463
ApoI RAATTY 2 cut(s) 363, 563
ArsI GACNNNNNNTTYG 2 cut(s) 519, 551
AspLEI GCGC 2 cut(s) 285, 307
AspS9I GGNCC 2 cut(s) 381, 535
AsuHPI GGTGA 2 cut(s) 500, 509
AvaII GGWCC 2 cut(s) 381, 535
BanI GGYRCC 1 cut(s) 304
BbvI GCAGC 2 cut(s) 214, 475
BccI CCATC 1 cut(s) 343
BciT130I CCWGG 1 cut(s) 334
BcoDI GTCTC 1 cut(s) 294
BfmI CTRYAG 3 cut(s) 161, 200, 582
BfoI RGCGCY 1 cut(s) 308
BisI GCNGC 2 cut(s) 203, 464
BlsI GCNGC 2 cut(s) 204, 465
Bme1390I CCNGG 1 cut(s) 334
Bme18I GGWCC 2 cut(s) 381, 535
BmgT120I GGNCC 2 cut(s) 381, 535
BmiI GGNNCC 3 cut(s) 306, 382, 536
BmrFI CCNGG 1 cut(s) 334
BmsI GCATC 1 cut(s) 338
BpuEI CTTGAG 1 cut(s) 309
Bsa29I ATCGAT 1 cut(s) 7
BsaHI GRCGYC 1 cut(s) 305
BsaJI CCNNGG 1 cut(s) 376
BsaXI ACNNNNNCTCC 2 cut(s) 67, 97
Bsc4I CCNNNNNNNGG 2 cut(s) 220, 544
Bse1I ACTGG 1 cut(s) 511
Bse3DI GCAATG 1 cut(s) 156
BseBI CCWGG 1 cut(s) 334
BseCI ATCGAT 1 cut(s) 7
BseDI CCNNGG 1 cut(s) 376
BseGI GGATG 2 cut(s) 329, 372
BseLI CCNNNNNNNGG 2 cut(s) 220, 544
BseMI GCAATG 1 cut(s) 156
BseMII CTCAG 1 cut(s) 48
BseNI ACTGG 1 cut(s) 511
BseRI GAGGAG 3 cut(s) 75, 82, 472
BseXI GCAGC 2 cut(s) 214, 475
BsgI GTGCAG 1 cut(s) 273
Bsh1236I CGCG 1 cut(s) 285
BshNI GGYRCC 1 cut(s) 304
BshVI ATCGAT 1 cut(s) 7
BslFI GGGAC 3 cut(s) 279, 394, 641
BslI CCNNNNNNNGG 2 cut(s) 220, 544
BsmAI GTCTC 1 cut(s) 294
BsmFI GGGAC 3 cut(s) 279, 394, 641
BspCNI CTCAG 1 cut(s) 47
BspDI ATCGAT 1 cut(s) 7
BspFNI CGCG 1 cut(s) 285
BspHI TCATGA 1 cut(s) 636
BspLI GGNNCC 3 cut(s) 306, 382, 536
BspMAI CTGCAG 3 cut(s) 165, 204, 586
BspT107I GGYRCC 1 cut(s) 304
BsrDI GCAATG 1 cut(s) 156
BsrI ACTGG 1 cut(s) 511
BssECI CCNNGG 1 cut(s) 376
BssNI GRCGYC 1 cut(s) 305
BssT1I CCWWGG 1 cut(s) 376
Bst2UI CCWGG 1 cut(s) 334
Bst4CI ACNGT 2 cut(s) 318, 431
Bst6I CTCTTC 1 cut(s) 184
BstACI GRCGYC 1 cut(s) 305
BstC8I GCNNGC 1 cut(s) 582
BstDEI CTNAG 1 cut(s) 34
BstF5I GGATG 2 cut(s) 329, 372
BstFNI CGCG 1 cut(s) 285
BstH2I RGCGCY 1 cut(s) 308
BstHHI GCGC 2 cut(s) 285, 307
BstMAI GTCTC 1 cut(s) 294
BstNI CCWGG 1 cut(s) 334
BstSCI CCNGG 1 cut(s) 332
BstSFI CTRYAG 3 cut(s) 161, 200, 582
BstUI CGCG 1 cut(s) 285
BstV1I GCAGC 2 cut(s) 214, 475
Bsu15I ATCGAT 1 cut(s) 7
BsuTUI ATCGAT 1 cut(s) 7
BtsCI GGATG 2 cut(s) 329, 372
BtsIMutI CAGTG 2 cut(s) 42, 504
Cac8I GCNNGC 1 cut(s) 582
CciI TCATGA 1 cut(s) 636
CfoI GCGC 2 cut(s) 285, 307
Cfr13I GGNCC 2 cut(s) 381, 535
ClaI ATCGAT 1 cut(s) 7
Csp6I GTAC 3 cut(s) 321, 432, 601
CspCI CAANNNNNGTGG 2 cut(s) 423, 458
CviAII CATG 3 cut(s) 517, 637, 646
CviJI RGCY 7 cut(s) 45, 225, 412, 466, 510, 580, 672
CviKI_1 RGCY 7 cut(s) 45, 225, 412, 466, 510, 580, 672
CviQI GTAC 3 cut(s) 321, 432, 601
DdeI CTNAG 1 cut(s) 34
DinI GGCGCC 1 cut(s) 306
Eam1104I CTCTTC 1 cut(s) 184
EarI CTCTTC 1 cut(s) 184
Eco130I CCWWGG 1 cut(s) 376
Eco47I GGWCC 2 cut(s) 381, 535
EcoO109I RGGNCCY 1 cut(s) 535
EcoRII CCWGG 1 cut(s) 332
EcoT14I CCWWGG 1 cut(s) 376
EgeI GGCGCC 1 cut(s) 306
EheI GGCGCC 1 cut(s) 306
ErhI CCWWGG 1 cut(s) 376
FaeI CATG 3 cut(s) 520, 640, 649
FalI AAGNNNNNCTT 2 cut(s) 522, 554
FaqI GGGAC 3 cut(s) 279, 394, 641
FatI CATG 3 cut(s) 516, 636, 645
Fnu4HI GCNGC 2 cut(s) 203, 464
FokI GGATG 2 cut(s) 316, 359
Fsp4HI GCNGC 2 cut(s) 203, 464
GlaI GCGC 2 cut(s) 284, 306
GluI GCNGC 2 cut(s) 203, 464
HaeII RGCGCY 1 cut(s) 308
HhaI GCGC 2 cut(s) 285, 307
Hin1I GRCGYC 1 cut(s) 305
Hin1II CATG 3 cut(s) 520, 640, 649
Hin6I GCGC 2 cut(s) 283, 305
HinP1I GCGC 2 cut(s) 283, 305
HincII GTYRAC 2 cut(s) 556, 571
HindII GTYRAC 2 cut(s) 556, 571
HinfI GANTC 6 cut(s) 4, 21, 257, 479, 633, 642
HpaI GTTAAC 1 cut(s) 556
HphI GGTGA 2 cut(s) 500, 509
Hpy166II GTNNAC 3 cut(s) 321, 556, 571
Hpy188I TCNGA 1 cut(s) 618
Hpy188III TCNNGA 2 cut(s) 18, 637
Hpy8I GTNNAC 3 cut(s) 321, 556, 571
HpyAV CCTTC 3 cut(s) 224, 477, 524
HpyCH4III ACNGT 2 cut(s) 318, 431
HpyCH4V TGCA 6 cut(s) 149, 163, 202, 254, 584, 659
HpyF3I CTNAG 1 cut(s) 34
Hsp92I GRCGYC 1 cut(s) 305
Hsp92II CATG 3 cut(s) 520, 640, 649
HspAI GCGC 2 cut(s) 283, 305
KasI GGCGCC 1 cut(s) 304
KspAI GTTAAC 1 cut(s) 556
LpnPI CCDG 8 cut(s) 173, 319, 346, 414, 471, 492, 570, 594
Lsp1109I GCAGC 2 cut(s) 214, 475
LweI GCATC 1 cut(s) 338
MaeIII GTNAC 3 cut(s) 53, 165, 497
MboII GAAGA 2 cut(s) 171, 539
MluCI AATT 5 cut(s) 13, 363, 447, 563, 688
Mly113I GGCGCC 1 cut(s) 305
MlyI GAGTC 1 cut(s) 473
MnlI CCTC 6 cut(s) 96, 103, 133, 148, 402, 493
MseI TTAA 4 cut(s) 98, 311, 342, 555
MslI CAYNNNNRTG 1 cut(s) 249
MspR9I CCNGG 1 cut(s) 334
MvaI CCWGG 1 cut(s) 334
MvnI CGCG 1 cut(s) 285
NarI GGCGCC 1 cut(s) 305
NlaIII CATG 3 cut(s) 520, 640, 649
NlaIV GGNNCC 3 cut(s) 306, 382, 536
NmuCI GTSAC 2 cut(s) 165, 497
PagI TCATGA 1 cut(s) 636
PfeI GAWTC 5 cut(s) 4, 21, 257, 633, 642
PkrI GCNGC 2 cut(s) 204, 465
PleI GAGTC 1 cut(s) 473
PluTI GGCGCC 1 cut(s) 308
PpsI GAGTC 1 cut(s) 473
PpuMI RGGWCCY 1 cut(s) 535
Psp5II RGGWCCY 1 cut(s) 535
Psp6I CCWGG 1 cut(s) 332
PspGI CCWGG 1 cut(s) 332
PspN4I GGNNCC 3 cut(s) 306, 382, 536
PspPI GGNCC 2 cut(s) 381, 535
PspPPI RGGWCCY 1 cut(s) 535
PsrI GAACNNNNNNTAC 2 cut(s) 381, 413
PstI CTGCAG 3 cut(s) 165, 204, 586
RsaI GTAC 3 cut(s) 322, 433, 602
RsaNI GTAC 3 cut(s) 321, 432, 601
RseI CAYNNNNRTG 1 cut(s) 249
SaqAI TTAA 4 cut(s) 98, 311, 342, 555
SatI GCNGC 2 cut(s) 203, 464
Sau96I GGNCC 2 cut(s) 381, 535
SbfI CCTGCAGG 1 cut(s) 586
SchI GAGTC 1 cut(s) 473
ScrFI CCNGG 1 cut(s) 334
SdaI CCTGCAGG 1 cut(s) 586
SetI ASST 1 cut(s) 338
SfaNI GCATC 1 cut(s) 338
SfcI CTRYAG 3 cut(s) 161, 200, 582
SfoI GGCGCC 1 cut(s) 306
SinI GGWCC 2 cut(s) 381, 535
SmiMI CAYNNNNRTG 1 cut(s) 249
SmlI CTYRAG 2 cut(s) 324, 673
SmoI CTYRAG 2 cut(s) 324, 673
Sse8387I CCTGCAGG 1 cut(s) 586
Sse9I AATT 5 cut(s) 13, 363, 447, 563, 688
SspDI GGCGCC 1 cut(s) 304
StyD4I CCNGG 1 cut(s) 332
StyI CCWWGG 1 cut(s) 376
TaaI ACNGT 2 cut(s) 318, 431
TaqI TCGA 1 cut(s) 7
TaqII GACCGA 1 cut(s) 398
TasI AATT 5 cut(s) 13, 363, 447, 563, 688
TatI WGTACW 2 cut(s) 320, 600
TfiI GAWTC 5 cut(s) 4, 21, 257, 633, 642
Tru1I TTAA 4 cut(s) 98, 311, 342, 555
Tru9I TTAA 4 cut(s) 98, 311, 342, 555
TscAI CASTG 2 cut(s) 42, 511
TseFI GTSAC 2 cut(s) 165, 497
TseI GCWGC 2 cut(s) 202, 463
Tsp45I GTSAC 2 cut(s) 165, 497
TspDTI ATGAA 9 cut(s) 17, 206, 233, 361, 402, 531, 625, 634, 670
TspGWI ACGGA 1 cut(s) 588
TspRI CASTG 2 cut(s) 42, 511
VpaK11BI GGWCC 2 cut(s) 381, 535
XapI RAATTY 2 cut(s) 363, 563
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.