pycom10g15830

RING-type E3 ubiquitin transferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0005506
Physical Location & Seq
Reverse (-)
19039178 .. 19040887
1710 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1230 bp
ATGTCCAATGGATGCGATAGTAACAAGAAAGGCCACTCATCACCAAAACAGTCATATAGTCATAGCAGGGGTGAATCAGCCACTAGTGCTGTTTCCAGTATTGATTATGCACCTCCAGCATCGATTAAGGTGTCAGGGATATCTAATAAGCATGAAAATGTTCGTAGGTTGTCTGGAGAAATCACATCTGAGCATCCGGTTGCTTCTACTCCAAAGAAACAATCAGCAATTCCCCCCGGGTTATCAGGAAAATTATCTCACATTTCCAAGACAAAAGCAGAAGGGGCATGGACTGATAGCCCCAGTTATCCTGATAGACAGCTGCTTCCATTTTATAGTTCAGGATCTGATGAGTTGACAACAGCATCCCATGTCAGGAATTTAATTGAAGACCTCAAGAGCCAGTCAAATGAAGTGCAAACTATGGCAGCAGAAGAGTTGCGGTTTCTTGGAAAGCACAATATGGAGAATCGAACTATTATAGGCCAGTGTGGTGCTATTACACCATTAGTTTCACTTCTATATTCAGGAGAGTTGACAACACAAGAGCATGCTGTGACAGCTCTTTTGAATTTATCAATTAATGAAGACAACAAAGCGATGATTGCAGAAGCAGGGGCAATTGATCCCCTAATCCATGTTCTAAAAACAGGAAGTGACGGAGCGAAAGAAAATTCTGCTGCAACTTTGTTCAGCCTCTCTGTATTGGACGAATACAAGGCAAAAATTGGTCGGTCTGGTGCAGTTAAGGCCTTGGTCGATCTTCTAGCCTCAGGGACTCTTCGGGGGAAGAAAGATGCTGGTACTGCATTGTTTAATCTGTCAATTTTTCATGAAAATAAAGCTCGCCTAGTTCAAGCTGGGGTTGTGAAGCATCTTATTGATTTGATGGACCCTGATTCTGGGATGGTTGACAAGGCCGTTGCTCTCCTGGCAAATTTGTCCACCATTGCCGAGGGCCGCATAGCAATTACACGGGAAGGGGGCATTCCGTTACTAGTTGAGATTGTTGAATCAGGTTCCCATAGAGGCAAGGAGAATGGTGCTTCCATATTGTTGCAGTTGTGCCTTCATAGTCCAAAGTTTTGCACCCTTGTTCTTCAGGAAGGAGCTGTTCCTCCCCTGGTTGCCTTATCTCAGTCTGGCACACCAAGAGCAAAGGAAAAGGCACAGCAGCTTCTCAGTCACTTCAGGAATCAGCGAGAAGGGGCTATGGGAAAGGGAAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Arm U-box

Protein Analysis

410

Amino Acids

43.43

Weight (kDa)

8.51

Isoelectric Point (pI)

38.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ARM_ARMC5 PF24768 117 - 297 1.2e-11 ARMC5
ARM_PUB PF25598 125 - 399 1.3e-25 Plant U-box protein ARM repeat region
Arm PF00514 156 - 193 1.7e-08 Armadillo/beta-catenin-like repeat
ARM_ARMC5 PF24768 189 - 358 4e-12 ARMC5
Arm PF00514 195 - 234 4.5e-06 Armadillo/beta-catenin-like repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 442, 961
AclWI GGATC 2 cut(s) 352, 620
AcsI RAATTY 4 cut(s) 379, 571, 673, 937
AcuI CTGAAG 2 cut(s) 1085, 1174
AfaI GTAC 1 cut(s) 805
AfiI CCNNNNNNNGG 2 cut(s) 375, 902
AgsI TTSAA 4 cut(s) 389, 571, 857, 1013
AhlI ACTAGT 2 cut(s) 83, 997
AjnI CCWGG 2 cut(s) 930, 1122
AluBI AGCT 6 cut(s) 322, 563, 845, 860, 1112, 1177
AluI AGCT 6 cut(s) 322, 563, 845, 860, 1112, 1177
AlwI GGATC 2 cut(s) 352, 620
AlwNI CAGNNNCTG 1 cut(s) 347
Ama87I CYCGRG 1 cut(s) 236
AoxI GGCC 5 cut(s) 31, 484, 750, 918, 958
ApeKI GCWGC 4 cut(s) 322, 428, 680, 1174
ApoI RAATTY 4 cut(s) 379, 571, 673, 937
ArsI GACNNNNNNTTYG 2 cut(s) 550, 582
AseI ATTAAT 1 cut(s) 582
Asp700I GAANNNNTTC 1 cut(s) 159
AspS9I GGNCC 2 cut(s) 892, 958
AsuC2I CCSGG 2 cut(s) 237, 238
AsuHPI GGTGA 2 cut(s) 33, 83
AvaI CYCGRG 1 cut(s) 236
AvaII GGWCC 1 cut(s) 892
AxyI CCTNAGG 1 cut(s) 772
BbsI GAAGAC 2 cut(s) 396, 594
BbvI GCAGC 4 cut(s) 309, 440, 667, 1186
BccI CCATC 2 cut(s) 883, 901
BceAI ACGGC 1 cut(s) 905
BciT130I CCWGG 2 cut(s) 932, 1124
BcnI CCSGG 2 cut(s) 237, 238
BcuI ACTAGT 2 cut(s) 83, 997
BfaI CTAG 4 cut(s) 84, 767, 851, 998
BisI GCNGC 5 cut(s) 323, 429, 681, 961, 1175
BlsI GCNGC 5 cut(s) 324, 430, 682, 962, 1176
Bme1390I CCNGG 4 cut(s) 237, 238, 932, 1124
Bme18I GGWCC 1 cut(s) 892
BmeT110I CYCGRG 1 cut(s) 236
BmgT120I GGNCC 2 cut(s) 892, 958
BmiI GGNNCC 2 cut(s) 894, 1021
BmrFI CCNGG 4 cut(s) 237, 238, 932, 1124
BmrI ACTGGG 1 cut(s) 297
BmsI GCATC 6 cut(s) 2, 128, 202, 374, 787, 883
BmuI ACTGGG 1 cut(s) 297
BpiI GAAGAC 2 cut(s) 396, 594
BpmI CTGGAG 2 cut(s) 99, 195
BpuEI CTTGAG 1 cut(s) 380
BpuMI CCSGG 2 cut(s) 237, 238
Bsa29I ATCGAT 1 cut(s) 122
BsaJI CCNNGG 5 cut(s) 235, 236, 753, 954, 1122
BsaWI WCCGGW 1 cut(s) 196
Bsc4I CCNNNNNNNGG 2 cut(s) 375, 902
Bse1I ACTGG 4 cut(s) 96, 303, 403, 487
Bse21I CCTNAGG 1 cut(s) 772
Bse3DI GCAATG 1 cut(s) 948
BseBI CCWGG 2 cut(s) 932, 1124
BseCI ATCGAT 1 cut(s) 122
BseDI CCNNGG 5 cut(s) 235, 236, 753, 954, 1122
BseGI GGATG 4 cut(s) 17, 193, 365, 912
BseLI CCNNNNNNNGG 2 cut(s) 375, 902
BseMI GCAATG 1 cut(s) 948
BseMII CTCAG 4 cut(s) 180, 786, 1151, 1195
BseNI ACTGG 4 cut(s) 96, 303, 403, 487
BseXI GCAGC 4 cut(s) 309, 440, 667, 1186
BseYI CCCAGC 1 cut(s) 860
BsgI GTGCAG 1 cut(s) 762
BshFI GGCC 5 cut(s) 33, 486, 752, 920, 960
BshVI ATCGAT 1 cut(s) 122
BsiHKCI CYCGRG 1 cut(s) 236
BsiSI CCGG 2 cut(s) 197, 237
BslFI GGGAC 1 cut(s) 790
BslI CCNNNNNNNGG 2 cut(s) 375, 902
BsmFI GGGAC 1 cut(s) 790
BsmI GAATGC 1 cut(s) 987
BsnI GGCC 5 cut(s) 33, 486, 752, 920, 960
BsoBI CYCGRG 1 cut(s) 236
Bsp143I GATC 3 cut(s) 344, 625, 760
BspACI CCGC 2 cut(s) 442, 961
BspANI GGCC 5 cut(s) 33, 486, 752, 920, 960
BspCNI CTCAG 4 cut(s) 181, 785, 1150, 1194
BspDI ATCGAT 1 cut(s) 122
BspHI TCATGA 1 cut(s) 832
BspLI GGNNCC 2 cut(s) 894, 1021
BspPI GGATC 2 cut(s) 352, 620
BsrDI GCAATG 1 cut(s) 948
BsrI ACTGG 4 cut(s) 96, 303, 403, 487
BssECI CCNNGG 5 cut(s) 235, 236, 753, 954, 1122
BssMI GATC 3 cut(s) 344, 625, 760
BssT1I CCWWGG 1 cut(s) 753
Bst2UI CCWGG 2 cut(s) 932, 1124
Bst4CI ACNGT 1 cut(s) 51
Bst6I CTCTTC 2 cut(s) 429, 786
BstC8I GCNNGC 2 cut(s) 552, 847
BstDEI CTNAG 4 cut(s) 189, 772, 1137, 1181
BstF5I GGATG 4 cut(s) 17, 193, 365, 912
BstKTI GATC 3 cut(s) 347, 628, 763
BstMBI GATC 3 cut(s) 344, 625, 760
BstMWI GCNNNNNNNGC 8 cut(s) 86, 116, 284, 560, 605, 749, 806, 932
BstNI CCWGG 2 cut(s) 932, 1124
BstNSI RCATGY 1 cut(s) 554
BstSCI CCNGG 4 cut(s) 235, 236, 930, 1122
BstV1I GCAGC 4 cut(s) 309, 440, 667, 1186
BstV2I GAAGAC 2 cut(s) 396, 594
BstX2I RGATCY 1 cut(s) 344
BstYI RGATCY 1 cut(s) 344
Bsu15I ATCGAT 1 cut(s) 122
Bsu36I CCTNAGG 1 cut(s) 772
BsuRI GGCC 5 cut(s) 33, 486, 752, 920, 960
BsuTUI ATCGAT 1 cut(s) 122
BtgZI GCGATG 1 cut(s) 614
BtsCI GGATG 4 cut(s) 17, 193, 365, 912
BtsIMutI CAGTG 1 cut(s) 494
Cac8I GCNNGC 2 cut(s) 552, 847
CaiI CAGNNNCTG 1 cut(s) 347
CciI TCATGA 1 cut(s) 832
Cfr13I GGNCC 2 cut(s) 892, 958
Cfr9I CCCGGG 1 cut(s) 236
ClaI ATCGAT 1 cut(s) 122
Csp6I GTAC 1 cut(s) 804
CviAII CATG 6 cut(s) 152, 288, 371, 551, 638, 833
CviQI GTAC 1 cut(s) 804
DdeI CTNAG 4 cut(s) 189, 772, 1137, 1181
DpnI GATC 3 cut(s) 346, 627, 762
DpnII GATC 3 cut(s) 344, 625, 760
Eam1104I CTCTTC 2 cut(s) 429, 786
EarI CTCTTC 2 cut(s) 429, 786
Eco130I CCWWGG 1 cut(s) 753
Eco147I AGGCCT 1 cut(s) 752
Eco32I GATATC 1 cut(s) 141
Eco47I GGWCC 1 cut(s) 892
Eco57I CTGAAG 2 cut(s) 1085, 1174
Eco81I CCTNAGG 1 cut(s) 772
Eco88I CYCGRG 1 cut(s) 236
EcoRII CCWGG 2 cut(s) 930, 1122
EcoRV GATATC 1 cut(s) 141
EcoT14I CCWWGG 1 cut(s) 753
ErhI CCWWGG 1 cut(s) 753
FaeI CATG 6 cut(s) 155, 291, 374, 554, 641, 836
FaqI GGGAC 1 cut(s) 790
FatI CATG 6 cut(s) 151, 287, 370, 550, 637, 832
Fnu4HI GCNGC 5 cut(s) 323, 429, 681, 961, 1175
FokI GGATG 4 cut(s) 24, 180, 352, 919
Fsp4HI GCNGC 5 cut(s) 323, 429, 681, 961, 1175
FspBI CTAG 4 cut(s) 84, 767, 851, 998
GluI GCNGC 5 cut(s) 323, 429, 681, 961, 1175
GsaI CCCAGC 1 cut(s) 864
GsuI CTGGAG 2 cut(s) 99, 195
HaeIII GGCC 5 cut(s) 33, 486, 752, 920, 960
HapII CCGG 2 cut(s) 197, 237
Hin1II CATG 6 cut(s) 155, 291, 374, 554, 641, 836
HincII GTYRAC 3 cut(s) 357, 537, 913
HindII GTYRAC 3 cut(s) 357, 537, 913
HinfI GANTC 6 cut(s) 74, 469, 778, 899, 1013, 1195
HpaII CCGG 2 cut(s) 197, 237
HphI GGTGA 2 cut(s) 33, 83
Hpy166II GTNNAC 4 cut(s) 357, 537, 913, 945
Hpy188I TCNGA 2 cut(s) 190, 349
Hpy8I GTNNAC 4 cut(s) 357, 537, 913, 945
HpyAV CCTTC 5 cut(s) 275, 974, 1079, 1100, 1199
HpyCH4III ACNGT 1 cut(s) 51
HpyCH4V TGCA 8 cut(s) 110, 418, 608, 683, 743, 809, 1060, 1089
HpyF10VI GCNNNNNNNGC 8 cut(s) 86, 116, 284, 560, 605, 749, 806, 932
HpyF3I CTNAG 4 cut(s) 189, 772, 1137, 1181
Hsp92II CATG 6 cut(s) 155, 291, 374, 554, 641, 836
Kzo9I GATC 3 cut(s) 344, 625, 760
LmnI GCTCC 2 cut(s) 662, 1109
Lsp1109I GCAGC 4 cut(s) 309, 440, 667, 1186
LweI GCATC 6 cut(s) 2, 128, 202, 374, 787, 883
MaeI CTAG 4 cut(s) 84, 767, 851, 998
MaeIII GTNAC 5 cut(s) 20, 556, 656, 993, 1184
MalI GATC 3 cut(s) 346, 627, 762
MboI GATC 3 cut(s) 344, 625, 760
MboII GAAGA 7 cut(s) 401, 446, 599, 755, 773, 802, 1091
MfeI CAATTG 1 cut(s) 621
MflI RGATCY 1 cut(s) 344
MlyI GAGTC 1 cut(s) 772
MnlI CCTC 7 cut(s) 123, 404, 707, 781, 949, 1022, 1128
MroXI GAANNNNTTC 1 cut(s) 159
MseI TTAA 5 cut(s) 126, 383, 582, 747, 816
MslI CAYNNNNRTG 1 cut(s) 156
MspA1I CMGCKG 1 cut(s) 322
MspI CCGG 2 cut(s) 197, 237
MspR9I CCNGG 4 cut(s) 237, 238, 932, 1124
MunI CAATTG 1 cut(s) 621
Mva1269I GAATGC 1 cut(s) 987
MvaI CCWGG 2 cut(s) 932, 1124
MwoI GCNNNNNNNGC 8 cut(s) 86, 116, 284, 560, 605, 749, 806, 932
NciI CCSGG 2 cut(s) 237, 238
NdeII GATC 3 cut(s) 344, 625, 760
NlaIII CATG 6 cut(s) 155, 291, 374, 554, 641, 836
NlaIV GGNNCC 2 cut(s) 894, 1021
NmeAIII GCCGAG 1 cut(s) 979
NmuCI GTSAC 3 cut(s) 556, 656, 1184
NspI RCATGY 1 cut(s) 554
PaeI GCATGC 1 cut(s) 554
PagI TCATGA 1 cut(s) 832
PceI AGGCCT 1 cut(s) 752
PctI GAATGC 1 cut(s) 987
PdmI GAANNNNTTC 1 cut(s) 159
PfeI GAWTC 5 cut(s) 74, 469, 899, 1013, 1195
PkrI GCNGC 5 cut(s) 324, 430, 682, 962, 1176
PleI GAGTC 1 cut(s) 772
PpsI GAGTC 1 cut(s) 772
PshBI ATTAAT 1 cut(s) 582
Psp6I CCWGG 2 cut(s) 930, 1122
PspFI CCCAGC 1 cut(s) 860
PspGI CCWGG 2 cut(s) 930, 1122
PspN4I GGNNCC 2 cut(s) 894, 1021
PspPI GGNCC 2 cut(s) 892, 958
PstNI CAGNNNCTG 1 cut(s) 347
PsuI RGATCY 1 cut(s) 344
PvuII CAGCTG 1 cut(s) 322
RsaI GTAC 1 cut(s) 805
RsaNI GTAC 1 cut(s) 804
RseI CAYNNNNRTG 1 cut(s) 156
SaqAI TTAA 5 cut(s) 126, 383, 582, 747, 816
SatI GCNGC 5 cut(s) 323, 429, 681, 961, 1175
Sau3AI GATC 3 cut(s) 344, 625, 760
Sau96I GGNCC 2 cut(s) 892, 958
SchI GAGTC 1 cut(s) 772
ScrFI CCNGG 4 cut(s) 237, 238, 932, 1124
SfaNI GCATC 6 cut(s) 2, 128, 202, 374, 787, 883
SinI GGWCC 1 cut(s) 892
SmaI CCCGGG 1 cut(s) 238
SmiMI CAYNNNNRTG 1 cut(s) 156
SmlI CTYRAG 1 cut(s) 395
SmoI CTYRAG 1 cut(s) 395
SpeI ACTAGT 2 cut(s) 83, 997
SphI GCATGC 1 cut(s) 554
SseBI AGGCCT 1 cut(s) 752
SsiI CCGC 2 cut(s) 442, 961
SspMI CTAG 4 cut(s) 84, 767, 851, 998
StuI AGGCCT 1 cut(s) 752
StyD4I CCNGG 4 cut(s) 235, 236, 930, 1122
StyI CCWWGG 1 cut(s) 753
TaaI ACNGT 1 cut(s) 51
TaqI TCGA 3 cut(s) 122, 472, 759
TaqII GACCGA 1 cut(s) 723
TauI GCSGC 1 cut(s) 963
TfiI GAWTC 5 cut(s) 74, 469, 899, 1013, 1195
Tru1I TTAA 5 cut(s) 126, 383, 582, 747, 816
Tru9I TTAA 5 cut(s) 126, 383, 582, 747, 816
TscAI CASTG 1 cut(s) 494
TseFI GTSAC 3 cut(s) 556, 656, 1184
TseI GCWGC 4 cut(s) 322, 428, 680, 1174
Tsp45I GTSAC 3 cut(s) 556, 656, 1184
TspDTI ATGAA 6 cut(s) 168, 426, 600, 821, 849, 1061
TspGWI ACGGA 2 cut(s) 675, 981
TspMI CCCGGG 1 cut(s) 236
TspRI CASTG 1 cut(s) 494
VpaK11BI GGWCC 1 cut(s) 892
VspI ATTAAT 1 cut(s) 582
XapI RAATTY 4 cut(s) 379, 571, 673, 937
XceI RCATGY 1 cut(s) 554
XmaI CCCGGG 1 cut(s) 236
XmnI GAANNNNTTC 1 cut(s) 159
XspI CTAG 4 cut(s) 84, 767, 851, 998
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.