pycom10g17880

Belongs to the phosphoglycerate kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0008242
Physical Location & Seq
Forward (+)
21049811 .. 21051019
1209 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 681 bp
ATGCAGAAGGAACTCGACTATCTTGTTGGTGCTGTGGCAAATCCTAAGAGGCCATTTGCTGCTATTGTTGGCGGTTCAAAGGTGTCAACCAAGATTGGAGTGATAGAATCCTTGTTGGCGAAGGTTAACGTTCTCTTGCTTGGTGGAGGAATGATCTTTACTTTCTACAAGGCCCAAGGCCATTCAGTTGGATCTTCCCTTGTGGAGGAGGACAAGCTTGATCTTGCAAAGTCGCTTCTTGAGAAGGCCAAGTCTAAGGGGGTTTCTATTCTCCTCCCAACTGATGTGGTTATTGCAGACAAATTTGCAGCTGATGCAAACTGCAAGGTTGTGCCAGCGTCTGCTATTCCAGATGGTTGGATGGGATTGGATATTGGACCGGACTCGATCAAAACTTTCAGTGAAGCTCTCGATACCACTCAGACTATTATCTGGAACGGACCTATGGGTGTTTTCGAATTTGAGAAGTTTGCTGCTGGGACTGAGGCAATAGCTAAGAAGCTTGCAGAGCTGAGCGGCAAGGGCGTGACAACAATCATCGGAGGTGGTGACTCAGTTGCCGCCGTTGAGAAGGCTGGGCTTGCTGAGAAGATGAGCCACATCTCCACCGGAGGCGGTGCAAGCTTAGAGCTCCTCGAAGGGAAAACACTACCCGGAGTCCTTGCTCTGGACGATGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0002237 GO:0003674 GO:0003824 GO:0004618 GO:0004672 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005773 GO:0005774 GO:0005829 GO:0005886 GO:0005911 GO:0005975 GO:0006082 GO:0006090 GO:0006091 GO:0006096 GO:0006139 GO:0006163 GO:0006164 GO:0006165 GO:0006464 GO:0006468 GO:0006725 GO:0006732 GO:0006733 GO:0006753 GO:0006754 GO:0006757 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0008150 GO:0008152 GO:0009056 GO:0009058 GO:0009108 GO:0009117 GO:0009123 GO:0009124 GO:0009126 GO:0009127 GO:0009132 GO:0009135 GO:0009141 GO:0009142 GO:0009144 GO:0009145 GO:0009150 GO:0009152 GO:0009156 GO:0009161 GO:0009165 GO:0009166 GO:0009167 GO:0009168 GO:0009179 GO:0009185 GO:0009199 GO:0009201 GO:0009205 GO:0009206 GO:0009259 GO:0009260 GO:0009266 GO:0009314 GO:0009408 GO:0009416 GO:0009506 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009605 GO:0009607 GO:0009617 GO:0009628 GO:0009743 GO:0009746 GO:0009749 GO:0009987 GO:0010033 GO:0016020 GO:0016052 GO:0016053 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0016774 GO:0017144 GO:0018130 GO:0019359 GO:0019362 GO:0019363 GO:0019438 GO:0019439 GO:0019538 GO:0019637 GO:0019693 GO:0019752 GO:0030054 GO:0031090 GO:0032787 GO:0034284 GO:0034404 GO:0034641 GO:0034654 GO:0034655 GO:0036211 GO:0042221 GO:0042866 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043436 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044260 GO:0044267 GO:0044270 GO:0044271 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0046031 GO:0046034 GO:0046390 GO:0046394 GO:0046434 GO:0046483 GO:0046496 GO:0046700 GO:0046939 GO:0048046 GO:0050896 GO:0051186 GO:0051188 GO:0051704 GO:0051707 GO:0055044 GO:0055086 GO:0071704 GO:0071944 GO:0072330 GO:0072521 GO:0072522 GO:0072524 GO:0072525 GO:0090407 GO:0098588 GO:0098805 GO:0140096 GO:1901135 GO:1901137 GO:1901292 GO:1901293 GO:1901360 GO:1901361 GO:1901362 GO:1901564 GO:1901566 GO:1901575 GO:1901576 GO:1901700
KEGG Pathways
Metabolic & Signaling
ko00010 ko00710 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 map00010 map00710 map01100 map01110 map01120 map01130 map01200 map01230
Pfam Domains
Protein Families
PGK

Protein Analysis

227

Amino Acids

23.26

Weight (kDa)

5.16

Isoelectric Point (pI)

28.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PGK PF00162 1 - 215 5.4e-89 Phosphoglycerate kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 516
AciI CCGC 4 cut(s) 72, 516, 561, 615
AclI AACGTT 1 cut(s) 129
AclWI GGATC 1 cut(s) 199
AcsI RAATTY 2 cut(s) 302, 458
AfiI CCNNNNNNNGG 2 cut(s) 205, 667
AgsI TTSAA 1 cut(s) 78
AluBI AGCT 8 cut(s) 217, 311, 407, 494, 502, 511, 624, 631
AluI AGCT 8 cut(s) 217, 311, 407, 494, 502, 511, 624, 631
Alw21I GWGCWC 1 cut(s) 633
AlwI GGATC 1 cut(s) 199
AlwNI CAGNNNCTG 1 cut(s) 341
AoxI GGCC 4 cut(s) 50, 171, 178, 246
ApeKI GCWGC 3 cut(s) 59, 308, 473
ApoI RAATTY 2 cut(s) 302, 458
AspS9I GGNCC 3 cut(s) 172, 377, 440
AsuC2I CCSGG 1 cut(s) 654
AsuHPI GGTGA 1 cut(s) 560
AsuII TTCGAA 1 cut(s) 456
AvaII GGWCC 2 cut(s) 377, 440
BanII GRGCYC 1 cut(s) 633
Bbv12I GWGCWC 1 cut(s) 633
BbvI GCAGC 3 cut(s) 46, 320, 460
BccI CCATC 2 cut(s) 347, 355
BceAI ACGGC 1 cut(s) 548
BcnI CCSGG 1 cut(s) 654
BisI GCNGC 5 cut(s) 60, 309, 474, 517, 561
BlpI GCTNAGC 1 cut(s) 512
BlsI GCNGC 5 cut(s) 61, 310, 475, 518, 562
Bme1390I CCNGG 1 cut(s) 654
Bme18I GGWCC 2 cut(s) 377, 440
BmgT120I GGNCC 3 cut(s) 172, 377, 440
BmrFI CCNGG 1 cut(s) 654
BmsI GCATC 2 cut(s) 304, 664
Bpu1102I GCTNAGC 1 cut(s) 512
Bpu14I TTCGAA 1 cut(s) 456
BpuEI CTTGAG 1 cut(s) 260
BpuMI CCSGG 1 cut(s) 654
BsaJI CCNNGG 1 cut(s) 175
BsaWI WCCGGW 2 cut(s) 379, 608
Bsc4I CCNNNNNNNGG 2 cut(s) 205, 667
BseDI CCNNGG 1 cut(s) 175
BseGI GGATG 1 cut(s) 366
BseLI CCNNNNNNNGG 2 cut(s) 205, 667
BseMII CTCAG 5 cut(s) 434, 474, 503, 567, 576
BseRI GAGGAG 3 cut(s) 221, 263, 623
BseXI GCAGC 3 cut(s) 46, 320, 460
BseYI CCCAGC 2 cut(s) 476, 575
BshFI GGCC 4 cut(s) 52, 173, 180, 248
BsiHKAI GWGCWC 1 cut(s) 633
BsiSI CCGG 3 cut(s) 380, 609, 654
BslFI GGGAC 1 cut(s) 493
BslI CCNNNNNNNGG 2 cut(s) 205, 667
BsmFI GGGAC 1 cut(s) 493
BsnI GGCC 4 cut(s) 52, 173, 180, 248
Bsp119I TTCGAA 1 cut(s) 456
Bsp1286I GDGCHC 1 cut(s) 633
Bsp143I GATC 4 cut(s) 153, 191, 220, 387
Bsp1720I GCTNAGC 1 cut(s) 512
BspACI CCGC 4 cut(s) 72, 516, 561, 615
BspANI GGCC 4 cut(s) 52, 173, 180, 248
BspCNI CTCAG 5 cut(s) 433, 475, 504, 566, 577
BspPI GGATC 1 cut(s) 199
BspT104I TTCGAA 1 cut(s) 456
BsrBI CCGCTC 1 cut(s) 516
BssECI CCNNGG 1 cut(s) 175
BssMI GATC 4 cut(s) 153, 191, 220, 387
BssT1I CCWWGG 1 cut(s) 175
BstAPI GCANNNNNTGC 1 cut(s) 314
BstBI TTCGAA 1 cut(s) 456
BstC8I GCNNGC 4 cut(s) 336, 504, 582, 622
BstDEI CTNAG 9 cut(s) 45, 255, 420, 483, 495, 512, 553, 585, 625
BstENI CCTNNNNNAGG 1 cut(s) 203
BstF5I GGATG 1 cut(s) 366
BstKTI GATC 4 cut(s) 156, 194, 223, 390
BstMBI GATC 4 cut(s) 153, 191, 220, 387
BstMWI GCNNNNNNNGC 5 cut(s) 314, 508, 522, 581, 621
BstSCI CCNGG 1 cut(s) 652
BstV1I GCAGC 3 cut(s) 46, 320, 460
BstX2I RGATCY 1 cut(s) 191
BstXI CCANNNNNNTGG 2 cut(s) 188, 357
BstYI RGATCY 1 cut(s) 191
BsuRI GGCC 4 cut(s) 52, 173, 180, 248
BtsCI GGATG 1 cut(s) 366
BtsIMutI CAGTG 1 cut(s) 406
Cac8I GCNNGC 4 cut(s) 336, 504, 582, 622
CaiI CAGNNNCTG 1 cut(s) 341
Cfr13I GGNCC 3 cut(s) 172, 377, 440
CseI GACGC 1 cut(s) 327
CspCI CAANNNNNGTGG 2 cut(s) 267, 302
DdeI CTNAG 9 cut(s) 45, 255, 420, 483, 495, 512, 553, 585, 625
DpnI GATC 4 cut(s) 155, 193, 222, 389
DpnII GATC 4 cut(s) 153, 191, 220, 387
Ecl136II GAGCTC 1 cut(s) 631
Eco130I CCWWGG 1 cut(s) 175
Eco24I GRGCYC 1 cut(s) 633
Eco47I GGWCC 2 cut(s) 377, 440
Eco53kI GAGCTC 1 cut(s) 631
EcoICRI GAGCTC 1 cut(s) 631
EcoNI CCTNNNNNAGG 1 cut(s) 203
EcoT14I CCWWGG 1 cut(s) 175
EcoT38I GRGCYC 1 cut(s) 633
ErhI CCWWGG 1 cut(s) 175
FaiI YATR 1 cut(s) 446
FaqI GGGAC 1 cut(s) 493
Fnu4HI GCNGC 5 cut(s) 60, 309, 474, 517, 561
FokI GGATG 1 cut(s) 373
FriOI GRGCYC 1 cut(s) 633
Fsp4HI GCNGC 5 cut(s) 60, 309, 474, 517, 561
GluI GCNGC 5 cut(s) 60, 309, 474, 517, 561
GsaI CCCAGC 2 cut(s) 480, 579
HaeIII GGCC 4 cut(s) 52, 173, 180, 248
HapII CCGG 3 cut(s) 380, 609, 654
HgaI GACGC 1 cut(s) 327
HincII GTYRAC 2 cut(s) 87, 127
HindII GTYRAC 2 cut(s) 87, 127
HindIII AAGCTT 3 cut(s) 215, 500, 622
HinfI GANTC 4 cut(s) 107, 383, 551, 657
HpaI GTTAAC 1 cut(s) 127
HpaII CCGG 3 cut(s) 380, 609, 654
HphI GGTGA 1 cut(s) 560
Hpy166II GTNNAC 2 cut(s) 87, 127
Hpy188I TCNGA 2 cut(s) 423, 542
Hpy188III TCNNGA 5 cut(s) 239, 350, 410, 433, 668
Hpy8I GTNNAC 2 cut(s) 87, 127
HpyAV CCTTC 4 cut(s) 115, 238, 565, 632
HpyCH4IV ACGT 1 cut(s) 129
HpyCH4V TGCA 8 cut(s) 4, 227, 296, 308, 317, 324, 506, 620
HpyF10VI GCNNNNNNNGC 5 cut(s) 314, 508, 522, 581, 621
HpyF3I CTNAG 9 cut(s) 45, 255, 420, 483, 495, 512, 553, 585, 625
HpySE526I ACGT 1 cut(s) 129
KspAI GTTAAC 1 cut(s) 127
Kzo9I GATC 4 cut(s) 153, 191, 220, 387
LmnI GCTCC 1 cut(s) 636
LpnPI CCDG 9 cut(s) 348, 363, 393, 418, 462, 561, 622, 653, 667
Lsp1109I GCAGC 3 cut(s) 46, 320, 460
LweI GCATC 2 cut(s) 304, 664
MaeII ACGT 1 cut(s) 129
MaeIII GTNAC 2 cut(s) 526, 548
MalI GATC 4 cut(s) 155, 193, 222, 389
MbiI CCGCTC 1 cut(s) 516
MboI GATC 4 cut(s) 153, 191, 220, 387
MboII GAAGA 2 cut(s) 186, 601
MflI RGATCY 1 cut(s) 191
MhlI GDGCHC 1 cut(s) 633
MluCI AATT 2 cut(s) 302, 458
MlyI GAGTC 3 cut(s) 377, 545, 666
MmeI TCCRAC 2 cut(s) 169, 338
MnlI CCTC 9 cut(s) 42, 140, 199, 202, 284, 478, 536, 605, 644
MseI TTAA 2 cut(s) 126, 679
MspA1I CMGCKG 1 cut(s) 311
MspI CCGG 3 cut(s) 380, 609, 654
MspR9I CCNGG 1 cut(s) 654
MwoI GCNNNNNNNGC 5 cut(s) 314, 508, 522, 581, 621
NciI CCSGG 1 cut(s) 654
NdeII GATC 4 cut(s) 153, 191, 220, 387
NmuCI GTSAC 2 cut(s) 526, 548
NspV TTCGAA 1 cut(s) 456
PfeI GAWTC 1 cut(s) 107
PkrI GCNGC 5 cut(s) 61, 310, 475, 518, 562
PleI GAGTC 3 cut(s) 377, 545, 665
PpsI GAGTC 3 cut(s) 377, 545, 665
Psp124BI GAGCTC 1 cut(s) 633
Psp1406I AACGTT 1 cut(s) 129
PspFI CCCAGC 2 cut(s) 476, 575
PspPI GGNCC 3 cut(s) 172, 377, 440
PstNI CAGNNNCTG 1 cut(s) 341
PsuI RGATCY 1 cut(s) 191
PvuII CAGCTG 1 cut(s) 311
SacI GAGCTC 1 cut(s) 633
SaqAI TTAA 2 cut(s) 126, 679
SatI GCNGC 5 cut(s) 60, 309, 474, 517, 561
Sau3AI GATC 4 cut(s) 153, 191, 220, 387
Sau96I GGNCC 3 cut(s) 172, 377, 440
SchI GAGTC 3 cut(s) 377, 545, 666
ScrFI CCNGG 1 cut(s) 654
SduI GDGCHC 1 cut(s) 633
SfaNI GCATC 2 cut(s) 304, 664
SfuI TTCGAA 1 cut(s) 456
SinI GGWCC 2 cut(s) 377, 440
SmlI CTYRAG 1 cut(s) 239
SmoI CTYRAG 1 cut(s) 239
Sse9I AATT 2 cut(s) 302, 458
SsiI CCGC 4 cut(s) 72, 516, 561, 615
SstI GAGCTC 1 cut(s) 633
StyD4I CCNGG 1 cut(s) 652
StyI CCWWGG 1 cut(s) 175
TaiI ACGT 1 cut(s) 132
TaqI TCGA 5 cut(s) 15, 386, 411, 456, 636
TasI AATT 2 cut(s) 302, 458
TauI GCSGC 2 cut(s) 519, 563
TfiI GAWTC 1 cut(s) 107
Tru1I TTAA 2 cut(s) 126, 679
Tru9I TTAA 2 cut(s) 126, 679
TscAI CASTG 1 cut(s) 406
TseFI GTSAC 2 cut(s) 526, 548
TseI GCWGC 3 cut(s) 59, 308, 473
Tsp45I GTSAC 2 cut(s) 526, 548
TspGWI ACGGA 1 cut(s) 453
TspRI CASTG 1 cut(s) 406
VpaK11BI GGWCC 2 cut(s) 377, 440
XagI CCTNNNNNAGG 1 cut(s) 203
XapI RAATTY 2 cut(s) 302, 458
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.