pycom10g18890

Methyladenine glycosylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0012860
Physical Location & Seq
Reverse (-)
22137761 .. 22139712
1952 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1170 bp
ATGTGTTCCTCGAAACCGAAATTGCAACGAAGTGTTAGTGTCTCTGCAATGTCCGCGAAACTGGTCAACCAGCGTCCAGTTCTCCAGCCAACCGGCAACCAATTTCCAAGCTTGGAACAAAAAAAGTCTCCTAAGAAAAGCTCCCAAGAAACTAATGTTCCAACTCCTCTTCCTTCACCAGTTCCAACTACTAAAACTAAGGCCTCACTTTCTCCTCCTATCTCTCCCAAGTTGCCTTCACCTCGACAGCCTGCGTTTAAGCGAGGTAAGGATCACAATCAGCTGAATTCTAGTGCTGAGAAGGTTGTAATGACGCCTCGTTGTGCAACCAAATCGACAAATTTGGTGAAAAAGTCGAAGAAATCTAGTGGCGTTGCACCTTCTGCTGAAAGTGTGTTGAAGAATATATCGTCTTTGATAGTAGAGGCACCAGGAAGCATAGCAACTGCAAGGAGGGAGCAAGTAGCAACAATGCAAGAACAGCGAAAGATGCGAATTGCGCATTACGGAAGAACAAAGTCTGCCAAGTACGAAGGGAAAGTTGCTCCTCTTGATTCTTCACAACCGAACGATTTCAGTCAAGAGAAAAAGCGATGCAGTTTCATCACCCCTAATTCAGACCCAATATATGTTGCATACCATGATGAAGAATGGGGAGTTCCTGTCCATGATGACAATATGTTGCTTGAATTGCTACTGTTAACTGGTGCTCAAGTGGGATCAGATTGGACTTCAGTCCTGAGGAGAAGAGAAACGTTGAGGGAGGCTTTTTCAGGGTTTGATGCAGATGTTATGGCTAAATTTAGCGAAAAGAAGATAATATCAGTGTGTTCTGATTCTGGCATAGATATAAGCCTTGTTCGAGGAGCTGTTGACAACGCTAAGAGAATTCTTGAGATAAAGAGGGAAGTGGGATCATTTGACAAGTACTTGTGGGGATTTGTGAATCACAAACCAATATCTACCCAATACAAGTCATGCCACAAGATACCTGTGAAGAACTCCAAGTCAGAGGCCATCAGCAAAGACATGGTTAGAAGGGGTTTTAGGCACGTCGGTCCCACTGTGATTCACTCCTTCATGCAAGCAGCTGGCCTCACTAACGACCACTTGATCACCTGCCCAAGGCACCTCCACTGCATTCCTTCACCATCATCACCCATGATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03410 map03410
Pfam Domains
Protein Families
Adenine_glyco

Protein Analysis

390

Amino Acids

42.92

Weight (kDa)

9.85

Isoelectric Point (pI)

54.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Adenine_glyco PF03352 205 - 377 2e-62 Methyladenine glycosylase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012860)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G44680
fragaria_vesca FvH4_3g13300
malus_domestica MD05G1243000.v1.1 MD10G1225100.v1.1
prunus_persica Prupe.4G120400_v2.0.a1
pyrus_communis pycom05g21960 pycom10g18890
rosa_chinensis RchiOBHm_Chr5g0021771
rosa_laevigata RLG00000032626
rosa_multiflora Rmu_ssc0000233.1_g000002
rosa_roxburghii Rroxscaffold_1G00056940
rosa_rugosa Rorug05G0069300 Rorug05G0069400
rosa_samantha Rh5AG157900 Rh5BG157400 Rh5CG171600 Rh5DG157700
rosa_wichuraiana Rw5G014060

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1127
Acc16I TGCGCA 1 cut(s) 501
Acc36I ACCTGC 1 cut(s) 1127
AccB1I GGYRCC 2 cut(s) 427, 1128
AccII CGCG 1 cut(s) 56
AciI CCGC 1 cut(s) 54
AclI AACGTT 1 cut(s) 755
AclWI GGATC 3 cut(s) 279, 727, 922
AcsI RAATTY 4 cut(s) 286, 340, 800, 888
AcuI CTGAAG 1 cut(s) 717
AcyI GRCGYC 1 cut(s) 314
AfaI GTAC 2 cut(s) 530, 929
AfiI CCNNNNNNNGG 1 cut(s) 1125
AgsI TTSAA 2 cut(s) 400, 689
AjiI CACGTC 1 cut(s) 1054
AjnI CCWGG 1 cut(s) 430
AluBI AGCT 5 cut(s) 111, 141, 283, 869, 1091
AluI AGCT 5 cut(s) 111, 141, 283, 869, 1091
Alw21I GWGCWC 1 cut(s) 712
Alw26I GTCTC 2 cut(s) 46, 132
AlwI GGATC 3 cut(s) 279, 727, 922
AoxI GGCC 3 cut(s) 201, 1014, 1093
ApeKI GCWGC 1 cut(s) 1088
ApoI RAATTY 4 cut(s) 286, 340, 800, 888
Asp700I GAANNNNTTC 1 cut(s) 572
AspLEI GCGC 1 cut(s) 502
AspS9I GGNCC 1 cut(s) 1058
AsuHPI GGTGA 7 cut(s) 168, 231, 358, 598, 1108, 1140, 1149
AvaII GGWCC 1 cut(s) 1058
AxyI CCTNAGG 1 cut(s) 740
BanI GGYRCC 2 cut(s) 427, 1128
Bbv12I GWGCWC 1 cut(s) 712
BbvI GCAGC 1 cut(s) 1100
BccI CCATC 2 cut(s) 1025, 1159
BcgI CGANNNNNNTGC 2 cut(s) 315, 349
BciT130I CCWGG 1 cut(s) 432
BclI TGATCA 1 cut(s) 1113
BcoDI GTCTC 2 cut(s) 46, 132
BfaI CTAG 2 cut(s) 291, 366
BfuAI ACCTGC 1 cut(s) 1127
BisI GCNGC 1 cut(s) 1089
BlsI GCNGC 1 cut(s) 1090
BmcAI AGTACT 1 cut(s) 929
Bme1390I CCNGG 1 cut(s) 432
Bme18I GGWCC 1 cut(s) 1058
BmgBI CACGTC 1 cut(s) 1054
BmgT120I GGNCC 1 cut(s) 1058
BmiI GGNNCC 3 cut(s) 429, 1060, 1130
BmrFI CCNGG 1 cut(s) 432
BmsI GCATC 3 cut(s) 480, 584, 772
BoxI GACNNNNGTC 1 cut(s) 734
BpmI CTGGAG 1 cut(s) 68
BpuEI CTTGAG 2 cut(s) 696, 914
BsaBI GATNNNNATC 1 cut(s) 276
BsaHI GRCGYC 1 cut(s) 314
BsaJI CCNNGG 1 cut(s) 1124
BsaXI ACNNNNNCTCC 2 cut(s) 648, 678
Bsc4I CCNNNNNNNGG 1 cut(s) 1125
Bse118I RCCGGY 1 cut(s) 92
Bse1I ACTGG 4 cut(s) 66, 77, 179, 709
Bse21I CCTNAGG 1 cut(s) 740
Bse3DI GCAATG 1 cut(s) 54
Bse8I GATNNNNATC 1 cut(s) 276
BseBI CCWGG 1 cut(s) 432
BseDI CCNNGG 1 cut(s) 1124
BseJI GATNNNNATC 1 cut(s) 276
BseLI CCNNNNNNNGG 1 cut(s) 1125
BseMI GCAATG 1 cut(s) 54
BseMII CTCAG 2 cut(s) 288, 731
BseNI ACTGG 4 cut(s) 66, 77, 179, 709
BseRI GAGGAG 5 cut(s) 156, 204, 537, 757, 879
BseXI GCAGC 1 cut(s) 1100
Bsh1236I CGCG 1 cut(s) 56
BshFI GGCC 3 cut(s) 203, 1016, 1095
BshNI GGYRCC 2 cut(s) 427, 1128
BsiHKAI GWGCWC 1 cut(s) 712
BsiSI CCGG 1 cut(s) 93
BslFI GGGAC 1 cut(s) 1044
BslI CCNNNNNNNGG 1 cut(s) 1125
BsmAI GTCTC 2 cut(s) 46, 132
BsmFI GGGAC 1 cut(s) 1044
BsmI GAATGC 1 cut(s) 1140
BsnI GGCC 3 cut(s) 203, 1016, 1095
Bsp1286I GDGCHC 1 cut(s) 712
Bsp143I GATC 4 cut(s) 271, 719, 914, 1113
BspACI CCGC 1 cut(s) 54
BspANI GGCC 3 cut(s) 203, 1016, 1095
BspCNI CTCAG 2 cut(s) 289, 732
BspFNI CGCG 1 cut(s) 56
BspLI GGNNCC 3 cut(s) 429, 1060, 1130
BspMI ACCTGC 1 cut(s) 1127
BspPI GGATC 3 cut(s) 279, 727, 922
BspT107I GGYRCC 2 cut(s) 427, 1128
BsrDI GCAATG 1 cut(s) 54
BsrFI RCCGGY 1 cut(s) 92
BsrI ACTGG 4 cut(s) 66, 77, 179, 709
BssAI RCCGGY 1 cut(s) 92
BssECI CCNNGG 1 cut(s) 1124
BssMI GATC 4 cut(s) 271, 719, 914, 1113
BssNI GRCGYC 1 cut(s) 314
BssT1I CCWWGG 1 cut(s) 1124
Bst2UI CCWGG 1 cut(s) 432
Bst4CI ACNGT 2 cut(s) 699, 1066
Bst6I CTCTTC 2 cut(s) 174, 742
BstACI GRCGYC 1 cut(s) 314
BstAPI GCANNNNNTGC 1 cut(s) 383
BstC8I GCNNGC 3 cut(s) 252, 1086, 1093
BstDEI CTNAG 5 cut(s) 132, 198, 297, 740, 882
BstENI CCTNNNNNAGG 1 cut(s) 1123
BstFNI CGCG 1 cut(s) 56
BstHHI GCGC 1 cut(s) 502
BstKTI GATC 4 cut(s) 274, 722, 917, 1116
BstMAI GTCTC 2 cut(s) 46, 132
BstMBI GATC 4 cut(s) 271, 719, 914, 1113
BstMWI GCNNNNNNNGC 6 cut(s) 53, 383, 481, 490, 499, 691
BstNI CCWGG 1 cut(s) 432
BstPAI GACNNNNGTC 1 cut(s) 734
BstSCI CCNGG 1 cut(s) 430
BstUI CGCG 1 cut(s) 56
BstV1I GCAGC 1 cut(s) 1100
Bsu36I CCTNAGG 1 cut(s) 740
BsuRI GGCC 3 cut(s) 203, 1016, 1095
BtgZI GCGATG 1 cut(s) 607
BtrI CACGTC 1 cut(s) 1054
BtsI GCAGTG 1 cut(s) 1135
BtsIMutI CAGTG 3 cut(s) 831, 1062, 1135
BveI ACCTGC 1 cut(s) 1127
Cac8I GCNNGC 3 cut(s) 252, 1086, 1093
CfoI GCGC 1 cut(s) 502
Cfr10I RCCGGY 1 cut(s) 92
Cfr13I GGNCC 1 cut(s) 1058
CseI GACGC 2 cut(s) 62, 322
Csp6I GTAC 2 cut(s) 529, 928
CviAII CATG 6 cut(s) 641, 668, 978, 1030, 1081, 1162
CviQI GTAC 2 cut(s) 529, 928
DdeI CTNAG 5 cut(s) 132, 198, 297, 740, 882
DpnI GATC 4 cut(s) 273, 721, 916, 1115
DpnII GATC 4 cut(s) 271, 719, 914, 1113
Eam1104I CTCTTC 2 cut(s) 174, 742
EarI CTCTTC 2 cut(s) 174, 742
Eco130I CCWWGG 1 cut(s) 1124
Eco147I AGGCCT 1 cut(s) 203
Eco47I GGWCC 1 cut(s) 1058
Eco57I CTGAAG 1 cut(s) 717
Eco81I CCTNAGG 1 cut(s) 740
EcoNI CCTNNNNNAGG 1 cut(s) 1123
EcoRI GAATTC 2 cut(s) 286, 888
EcoRII CCWGG 1 cut(s) 430
EcoT14I CCWWGG 1 cut(s) 1124
ErhI CCWWGG 1 cut(s) 1124
FaeI CATG 6 cut(s) 644, 671, 981, 1033, 1084, 1165
FaqI GGGAC 1 cut(s) 1044
FatI CATG 6 cut(s) 640, 667, 977, 1029, 1080, 1161
FbaI TGATCA 1 cut(s) 1113
Fnu4HI GCNGC 1 cut(s) 1089
Fsp4HI GCNGC 1 cut(s) 1089
FspBI CTAG 2 cut(s) 291, 366
FspI TGCGCA 1 cut(s) 501
GlaI GCGC 1 cut(s) 501
GluI GCNGC 1 cut(s) 1089
GsuI CTGGAG 1 cut(s) 68
HaeIII GGCC 3 cut(s) 203, 1016, 1095
HapII CCGG 1 cut(s) 93
HgaI GACGC 2 cut(s) 62, 322
HhaI GCGC 1 cut(s) 502
Hin1I GRCGYC 1 cut(s) 314
Hin1II CATG 6 cut(s) 644, 671, 981, 1033, 1084, 1165
Hin6I GCGC 1 cut(s) 500
HinP1I GCGC 1 cut(s) 500
HincII GTYRAC 3 cut(s) 67, 702, 874
HindII GTYRAC 3 cut(s) 67, 702, 874
HindIII AAGCTT 1 cut(s) 109
HinfI GANTC 4 cut(s) 554, 836, 946, 1069
HpaI GTTAAC 1 cut(s) 702
HpaII CCGG 1 cut(s) 93
HphI GGTGA 7 cut(s) 168, 231, 358, 598, 1108, 1140, 1149
Hpy166II GTNNAC 3 cut(s) 67, 702, 874
Hpy188I TCNGA 4 cut(s) 619, 724, 835, 1012
Hpy188III TCNNGA 4 cut(s) 551, 581, 739, 893
Hpy8I GTNNAC 3 cut(s) 67, 702, 874
Hpy99I CGWCG 1 cut(s) 1058
HpyAV CCTTC 8 cut(s) 183, 246, 295, 390, 527, 1032, 1087, 1155
HpyCH4III ACNGT 2 cut(s) 699, 1066
HpyCH4IV ACGT 2 cut(s) 755, 1053
HpyF10VI GCNNNNNNNGC 6 cut(s) 53, 383, 481, 490, 499, 691
HpyF3I CTNAG 5 cut(s) 132, 198, 297, 740, 882
HpySE526I ACGT 2 cut(s) 755, 1053
Hsp92I GRCGYC 1 cut(s) 314
Hsp92II CATG 6 cut(s) 644, 671, 981, 1033, 1084, 1165
HspAI GCGC 1 cut(s) 500
Ksp22I TGATCA 1 cut(s) 1113
KspAI GTTAAC 1 cut(s) 702
Kzo9I GATC 4 cut(s) 271, 719, 914, 1113
LmnI GCTCC 4 cut(s) 146, 457, 550, 866
Lsp1109I GCAGC 1 cut(s) 1100
LweI GCATC 3 cut(s) 480, 584, 772
MaeI CTAG 2 cut(s) 291, 366
MaeII ACGT 2 cut(s) 755, 1053
MalI GATC 4 cut(s) 273, 721, 916, 1115
MboI GATC 4 cut(s) 271, 719, 914, 1113
MboII GAAGA 9 cut(s) 161, 370, 412, 522, 549, 659, 759, 826, 1009
MhlI GDGCHC 1 cut(s) 712
MluCI AATT 9 cut(s) 20, 101, 286, 340, 495, 613, 689, 800, 888
MmeI TCCRAC 2 cut(s) 185, 209
MroXI GAANNNNTTC 1 cut(s) 572
MseI TTAA 2 cut(s) 258, 701
MspA1I CMGCKG 2 cut(s) 283, 1091
MspI CCGG 1 cut(s) 93
MspR9I CCNGG 1 cut(s) 432
Mva1269I GAATGC 1 cut(s) 1140
MvaI CCWGG 1 cut(s) 432
MvnI CGCG 1 cut(s) 56
MwoI GCNNNNNNNGC 6 cut(s) 53, 383, 481, 490, 499, 691
NdeII GATC 4 cut(s) 271, 719, 914, 1113
NlaIII CATG 6 cut(s) 644, 671, 981, 1033, 1084, 1165
NlaIV GGNNCC 3 cut(s) 429, 1060, 1130
NsbI TGCGCA 1 cut(s) 501
PaqCI CACCTGC 1 cut(s) 1127
PceI AGGCCT 1 cut(s) 203
PctI GAATGC 1 cut(s) 1140
PdmI GAANNNNTTC 1 cut(s) 572
PfeI GAWTC 4 cut(s) 554, 836, 946, 1069
PkrI GCNGC 1 cut(s) 1090
PshAI GACNNNNGTC 1 cut(s) 734
Psp1406I AACGTT 1 cut(s) 755
Psp6I CCWGG 1 cut(s) 430
PspGI CCWGG 1 cut(s) 430
PspN4I GGNNCC 3 cut(s) 429, 1060, 1130
PspPI GGNCC 1 cut(s) 1058
PvuII CAGCTG 2 cut(s) 283, 1091
RsaI GTAC 2 cut(s) 530, 929
RsaNI GTAC 2 cut(s) 529, 928
SaqAI TTAA 2 cut(s) 258, 701
SatI GCNGC 1 cut(s) 1089
Sau3AI GATC 4 cut(s) 271, 719, 914, 1113
Sau96I GGNCC 1 cut(s) 1058
ScaI AGTACT 1 cut(s) 929
ScrFI CCNGG 1 cut(s) 432
SduI GDGCHC 1 cut(s) 712
SfaNI GCATC 3 cut(s) 480, 584, 772
SinI GGWCC 1 cut(s) 1058
SmlI CTYRAG 2 cut(s) 711, 893
SmoI CTYRAG 2 cut(s) 711, 893
Sse9I AATT 9 cut(s) 20, 101, 286, 340, 495, 613, 689, 800, 888
SseBI AGGCCT 1 cut(s) 203
SsiI CCGC 1 cut(s) 54
SspMI CTAG 2 cut(s) 291, 366
StuI AGGCCT 1 cut(s) 203
StyD4I CCNGG 1 cut(s) 430
StyI CCWWGG 1 cut(s) 1124
TaaI ACNGT 2 cut(s) 699, 1066
TaiI ACGT 2 cut(s) 758, 1056
TaqI TCGA 5 cut(s) 11, 244, 335, 356, 862
TaqII GACCGA 1 cut(s) 1046
TasI AATT 9 cut(s) 20, 101, 286, 340, 495, 613, 689, 800, 888
TatI WGTACW 1 cut(s) 927
TfiI GAWTC 4 cut(s) 554, 836, 946, 1069
Tru1I TTAA 2 cut(s) 258, 701
Tru9I TTAA 2 cut(s) 258, 701
TscAI CASTG 3 cut(s) 831, 1069, 1142
TseI GCWGC 1 cut(s) 1088
TspDTI ATGAA 3 cut(s) 592, 660, 1069
TspGWI ACGGA 1 cut(s) 522
TspRI CASTG 3 cut(s) 831, 1069, 1142
VpaK11BI GGWCC 1 cut(s) 1058
XagI CCTNNNNNAGG 1 cut(s) 1123
XapI RAATTY 4 cut(s) 286, 340, 800, 888
XmnI GAANNNNTTC 1 cut(s) 572
XspI CTAG 2 cut(s) 291, 366
ZrmI AGTACT 1 cut(s) 929
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.