pycom10g19760

GDP-fucose protein O-fucosyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0006563
Physical Location & Seq
Reverse (-)
22823001 .. 22824353
1353 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1353 bp
ATGGACGTGCCTCCTTACTGTAAGAATTTCAAAGTCCTCGGTTGCCATTTGAATTCGTTCGCATGCAAATGTGTTGTTTTAGCTGTGACTGCTCTGGTAATTAGAGCTGTTCTGCTTCCTACATTCTCCATCTTTGCTGGAACTGAACAGGACAACTTGGTATTCATCGGTAATCTCTCGTTGTCATTCGGTCAGAAATCTGGGATCCGAAAAGATAAGTTTCTGGAGGTTCCTCAAATCGTTTGGGGACTAAACAACCAGAAAGTAGCGTTTGCAAGAGCTTGTCTGACCGCAAGGCTTCTGAACCGGACGCTTTTGATGCCTAGCTTGAGTGCTTCCCTGTTTTACAAGGAAGTTGAGCTCTTGGAGCCGATTTCCTTCGATAAGGTGTTCCAGTTCAAGAAGTTTAATTCTCTCTGTAATGGATTCGTTCAATTGGGTGAACTTTCAGATGTTAGAAACCGAACAGGAGCATTTGAGCTTCAGAAAGGAAGCGGAAGGAGGTGGACTCCCGAGAGGGACATGGATCAGTTGAAACAGCATAATGAGGATCCATACAATGAGTACGAGGTGATTCGAATCGTAGGAAAGAATCCGTTTCTGTGGCACGATCATTGGCCTGTGAAGGACTATGCGAAAATCCTTGAGTGCATGGTTTTGGTTGATGAGATAATGAATGAAGCGGATAAAGTTGTGTCACGGATTAGAGAGATAGGAGCAGCACAAGTAAGTAGTCAGAAAGAGTTTGCGCAAGTTGGCATTAGCCCGGCAGAGAATTCTCTCGTTCAGCCGGTGCCTTATGTGGCTGTACATATGAGGATAGAGATAGATTGGATGATTCATTGTAAGAAGCTAGAGCAAAGATCAATCATAACCCAAATTTGTAGTAGCAAGGAGGAGATCATGGAAAGAGTAGGCAACATTGTTGGCCTCAAAACGCCTGTCGTTGTTTATCTAGCTATAGCTGATAGTCTTCTTCATGATCCGTCTATTCTAAACGGCTGGAAAGAAGGCCTGATTCCTTTCGAGAAGAAGAAATTGGGCGTCGAAGGAAATTACAAGAAGTTCTCTTATCTCATTCAGTCTGCAATCGACTACGAAGTGTGTTTAAGGGCTGATGTCTTTGTGGGAAACAGTTTCTCAACATTTTCGAATCTTATAGTTCTGGATCGAACGCAGAAGCTCATTAGGACGGGCGTCACAAGCTCGTGTGGTGTGGATGTAAGGTGGCCTTCTTACGCATACAACATATTAGGGGAATCCAAAGGCCCTCGAAGATGGATGACAAATATGTCTGGCTCAAGTCTTCAAGCAATTAGCTATGGCTCCAACGACATCTCCTGTCGAGTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
O-FucT

Protein Analysis

451

Amino Acids

50.81

Weight (kDa)

8.6

Isoelectric Point (pI)

42.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
O-FucT PF10250 83 - 391 1.3e-24 GDP-fucose protein O-fucosyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1291
Acc16I TGCGCA 1 cut(s) 750
AccB1I GGYRCC 1 cut(s) 793
AciI CCGC 3 cut(s) 291, 495, 683
AclWI GGATC 7 cut(s) 199, 212, 534, 545, 558, 977, 1176
AcsI RAATTY 4 cut(s) 25, 52, 775, 879
AcuI CTGAAG 1 cut(s) 467
AcyI GRCGYC 2 cut(s) 1044, 1197
AfaI GTAC 2 cut(s) 566, 810
AgsI TTSAA 6 cut(s) 31, 52, 400, 434, 535, 1310
AjiI CACGTC 1 cut(s) 7
AjuI GAANNNNNNNTTGG 2 cut(s) 1022, 1054
Alw21I GWGCWC 1 cut(s) 363
AlwI GGATC 7 cut(s) 199, 212, 534, 545, 558, 977, 1176
Ama87I CYCGRG 1 cut(s) 512
AoxI GGCC 5 cut(s) 617, 928, 1012, 1229, 1267
ApeKI GCWGC 1 cut(s) 719
ApoI RAATTY 4 cut(s) 25, 52, 775, 879
ArsI GACNNNNNNTTYG 2 cut(s) 927, 959
Asp700I GAANNNNTTC 1 cut(s) 56
AspLEI GCGC 1 cut(s) 751
AspS9I GGNCC 1 cut(s) 1268
AsuC2I CCSGG 1 cut(s) 767
AsuHPI GGTGA 2 cut(s) 452, 583
AsuII TTCGAA 2 cut(s) 577, 1151
AvaI CYCGRG 1 cut(s) 512
BamHI GGATCC 2 cut(s) 204, 550
BanI GGYRCC 1 cut(s) 793
BanII GRGCYC 1 cut(s) 363
BauI CACGAG 1 cut(s) 1207
BbsI GAAGAC 2 cut(s) 965, 1298
Bbv12I GWGCWC 1 cut(s) 363
BbvI GCAGC 1 cut(s) 731
BccI CCATC 2 cut(s) 137, 1272
BceAI ACGGC 1 cut(s) 1015
BcnI CCSGG 1 cut(s) 767
BfaI CTAG 3 cut(s) 324, 854, 956
BfmI CTRYAG 1 cut(s) 960
BisI GCNGC 1 cut(s) 720
BlsI GCNGC 1 cut(s) 721
Bme1390I CCNGG 1 cut(s) 767
BmeT110I CYCGRG 1 cut(s) 512
BmgBI CACGTC 1 cut(s) 7
BmgT120I GGNCC 1 cut(s) 1268
BmiI GGNNCC 6 cut(s) 206, 231, 369, 552, 795, 1327
BmrFI CCNGG 1 cut(s) 767
BmsI GCATC 1 cut(s) 309
BoxI GACNNNNGTC 1 cut(s) 1196
BpiI GAAGAC 2 cut(s) 965, 1298
BplI GAGNNNNNCTC 2 cut(s) 493, 525
BpmI CTGGAG 1 cut(s) 245
Bpu14I TTCGAA 2 cut(s) 577, 1151
BpuEI CTTGAG 3 cut(s) 349, 665, 1285
BpuMI CCSGG 1 cut(s) 767
BsaBI GATNNNNATC 1 cut(s) 578
BsaHI GRCGYC 2 cut(s) 1044, 1197
BsaJI CCNNGG 1 cut(s) 37
BsaWI WCCGGW 1 cut(s) 306
BsaXI ACNNNNNCTCC 2 cut(s) 1322, 1352
Bse118I RCCGGY 1 cut(s) 790
Bse1I ACTGG 1 cut(s) 394
Bse8I GATNNNNATC 1 cut(s) 578
BseDI CCNNGG 1 cut(s) 37
BseGI GGATG 3 cut(s) 840, 1225, 1287
BseJI GATNNNNATC 1 cut(s) 578
BseNI ACTGG 1 cut(s) 394
BseRI GAGGAG 1 cut(s) 911
BseXI GCAGC 1 cut(s) 731
BshFI GGCC 5 cut(s) 619, 930, 1014, 1231, 1269
BshNI GGYRCC 1 cut(s) 793
BsiHKAI GWGCWC 1 cut(s) 363
BsiHKCI CYCGRG 1 cut(s) 512
BsiSI CCGG 3 cut(s) 307, 767, 791
BslFI GGGAC 2 cut(s) 261, 533
BsmFI GGGAC 2 cut(s) 261, 533
BsnI GGCC 5 cut(s) 619, 930, 1014, 1231, 1269
BsoBI CYCGRG 1 cut(s) 512
Bsp119I TTCGAA 2 cut(s) 577, 1151
Bsp1286I GDGCHC 1 cut(s) 363
Bsp1407I TGTACA 1 cut(s) 808
Bsp143I GATC 8 cut(s) 204, 526, 550, 610, 863, 900, 982, 1168
BspACI CCGC 3 cut(s) 291, 495, 683
BspANI GGCC 5 cut(s) 619, 930, 1014, 1231, 1269
BspHI TCATGA 1 cut(s) 979
BspLI GGNNCC 6 cut(s) 206, 231, 369, 552, 795, 1327
BspPI GGATC 7 cut(s) 199, 212, 534, 545, 558, 977, 1176
BspT104I TTCGAA 2 cut(s) 577, 1151
BspT107I GGYRCC 1 cut(s) 793
BsrFI RCCGGY 1 cut(s) 790
BsrGI TGTACA 1 cut(s) 808
BsrI ACTGG 1 cut(s) 394
BssAI RCCGGY 1 cut(s) 790
BssECI CCNNGG 1 cut(s) 37
BssMI GATC 8 cut(s) 204, 526, 550, 610, 863, 900, 982, 1168
BssNI GRCGYC 2 cut(s) 1044, 1197
BssSI CACGAG 1 cut(s) 1207
Bst2BI CACGAG 1 cut(s) 1207
Bst4CI ACNGT 2 cut(s) 20, 1136
BstACI GRCGYC 2 cut(s) 1044, 1197
BstAUI TGTACA 1 cut(s) 808
BstBI TTCGAA 2 cut(s) 577, 1151
BstC8I GCNNGC 1 cut(s) 64
BstF5I GGATG 3 cut(s) 840, 1225, 1287
BstHHI GCGC 1 cut(s) 751
BstKTI GATC 8 cut(s) 207, 529, 553, 613, 866, 903, 985, 1171
BstMBI GATC 8 cut(s) 204, 526, 550, 610, 863, 900, 982, 1168
BstMWI GCNNNNNNNGC 4 cut(s) 89, 319, 367, 1203
BstNSI RCATGY 1 cut(s) 66
BstPAI GACNNNNGTC 1 cut(s) 1196
BstSCI CCNGG 1 cut(s) 765
BstSFI CTRYAG 1 cut(s) 960
BstV1I GCAGC 1 cut(s) 731
BstV2I GAAGAC 2 cut(s) 965, 1298
BstX2I RGATCY 2 cut(s) 204, 550
BstYI RGATCY 2 cut(s) 204, 550
BsuRI GGCC 5 cut(s) 619, 930, 1014, 1231, 1269
BtrI CACGTC 1 cut(s) 7
BtsCI GGATG 3 cut(s) 840, 1225, 1287
Cac8I GCNNGC 1 cut(s) 64
CciI TCATGA 1 cut(s) 979
CfoI GCGC 1 cut(s) 751
Cfr10I RCCGGY 1 cut(s) 790
Cfr13I GGNCC 1 cut(s) 1268
CseI GACGC 3 cut(s) 319, 1033, 1186
Csp6I GTAC 2 cut(s) 565, 809
CviAII CATG 5 cut(s) 63, 523, 652, 904, 980
CviQI GTAC 2 cut(s) 565, 809
DpnI GATC 8 cut(s) 206, 528, 552, 612, 865, 902, 984, 1170
DpnII GATC 8 cut(s) 204, 526, 550, 610, 863, 900, 982, 1168
DrdI GACNNNNNNGTC 1 cut(s) 1291
DseDI GACNNNNNNGTC 1 cut(s) 1291
Ecl136II GAGCTC 1 cut(s) 361
Eco147I AGGCCT 1 cut(s) 1014
Eco24I GRGCYC 1 cut(s) 363
Eco53kI GAGCTC 1 cut(s) 361
Eco57I CTGAAG 1 cut(s) 467
Eco88I CYCGRG 1 cut(s) 512
EcoICRI GAGCTC 1 cut(s) 361
EcoO109I RGGNCCY 1 cut(s) 1268
EcoRI GAATTC 2 cut(s) 52, 775
EcoT38I GRGCYC 1 cut(s) 363
FaeI CATG 5 cut(s) 66, 526, 655, 907, 983
FalI AAGNNNNNCTT 2 cut(s) 1216, 1248
FaqI GGGAC 2 cut(s) 261, 533
FatI CATG 5 cut(s) 62, 522, 651, 903, 979
FauNDI CATATG 1 cut(s) 813
Fnu4HI GCNGC 1 cut(s) 720
FokI GGATG 3 cut(s) 847, 1232, 1294
FriOI GRGCYC 1 cut(s) 363
Fsp4HI GCNGC 1 cut(s) 720
FspBI CTAG 3 cut(s) 324, 854, 956
FspI TGCGCA 1 cut(s) 750
GlaI GCGC 1 cut(s) 750
GluI GCNGC 1 cut(s) 720
GsuI CTGGAG 1 cut(s) 245
HaeIII GGCC 5 cut(s) 619, 930, 1014, 1231, 1269
HapII CCGG 3 cut(s) 307, 767, 791
HgaI GACGC 3 cut(s) 319, 1033, 1186
HhaI GCGC 1 cut(s) 751
Hin1I GRCGYC 2 cut(s) 1044, 1197
Hin1II CATG 5 cut(s) 66, 526, 655, 907, 983
Hin6I GCGC 1 cut(s) 749
HinP1I GCGC 1 cut(s) 749
HpaII CCGG 3 cut(s) 307, 767, 791
HphI GGTGA 2 cut(s) 452, 583
Hpy166II GTNNAC 2 cut(s) 443, 507
Hpy188I TCNGA 8 cut(s) 195, 209, 288, 303, 451, 486, 738, 1352
Hpy188III TCNNGA 6 cut(s) 224, 400, 512, 980, 1027, 1166
Hpy8I GTNNAC 2 cut(s) 443, 507
Hpy99I CGWCG 1 cut(s) 1049
HpyAV CCTTC 6 cut(s) 388, 492, 619, 1004, 1043, 1242
HpyCH4III ACNGT 2 cut(s) 20, 1136
HpyCH4IV ACGT 1 cut(s) 6
HpyCH4V TGCA 4 cut(s) 66, 275, 651, 1088
HpyF10VI GCNNNNNNNGC 4 cut(s) 89, 319, 367, 1203
HpySE526I ACGT 1 cut(s) 6
Hsp92I GRCGYC 2 cut(s) 1044, 1197
Hsp92II CATG 5 cut(s) 66, 526, 655, 907, 983
HspAI GCGC 1 cut(s) 749
Kzo9I GATC 8 cut(s) 204, 526, 550, 610, 863, 900, 982, 1168
LmnI GCTCC 4 cut(s) 367, 470, 716, 1331
Lsp1109I GCAGC 1 cut(s) 731
LweI GCATC 1 cut(s) 309
MaeI CTAG 3 cut(s) 324, 854, 956
MaeII ACGT 1 cut(s) 6
MaeIII GTNAC 3 cut(s) 85, 696, 1198
MalI GATC 8 cut(s) 206, 528, 552, 612, 865, 902, 984, 1170
MboI GATC 8 cut(s) 204, 526, 550, 610, 863, 900, 982, 1168
MboII GAAGA 6 cut(s) 965, 968, 1042, 1045, 1287, 1298
MfeI CAATTG 1 cut(s) 434
MflI RGATCY 2 cut(s) 204, 550
MhlI GDGCHC 1 cut(s) 363
MlyI GAGTC 1 cut(s) 502
MmeI TCCRAC 1 cut(s) 1353
MroXI GAANNNNTTC 1 cut(s) 56
MseI TTAA 2 cut(s) 408, 1109
MslI CAYNNNNRTG 1 cut(s) 67
MspI CCGG 3 cut(s) 307, 767, 791
MspR9I CCNGG 1 cut(s) 767
MunI CAATTG 1 cut(s) 434
MwoI GCNNNNNNNGC 4 cut(s) 89, 319, 367, 1203
NciI CCSGG 1 cut(s) 767
NdeI CATATG 1 cut(s) 813
NdeII GATC 8 cut(s) 204, 526, 550, 610, 863, 900, 982, 1168
NlaIII CATG 5 cut(s) 66, 526, 655, 907, 983
NlaIV GGNNCC 6 cut(s) 206, 231, 369, 552, 795, 1327
NmuCI GTSAC 3 cut(s) 85, 696, 1198
NsbI TGCGCA 1 cut(s) 750
NspI RCATGY 1 cut(s) 66
NspV TTCGAA 2 cut(s) 577, 1151
PaeI GCATGC 1 cut(s) 66
PagI TCATGA 1 cut(s) 979
PceI AGGCCT 1 cut(s) 1014
PdmI GAANNNNTTC 1 cut(s) 56
PfeI GAWTC 8 cut(s) 426, 574, 579, 592, 838, 1018, 1153, 1259
PkrI GCNGC 1 cut(s) 721
PleI GAGTC 1 cut(s) 502
PpsI GAGTC 1 cut(s) 502
PshAI GACNNNNGTC 1 cut(s) 1196
Psp124BI GAGCTC 1 cut(s) 363
PspN4I GGNNCC 6 cut(s) 206, 231, 369, 552, 795, 1327
PspPI GGNCC 1 cut(s) 1268
PsuI RGATCY 2 cut(s) 204, 550
RsaI GTAC 2 cut(s) 566, 810
RsaNI GTAC 2 cut(s) 565, 809
RseI CAYNNNNRTG 1 cut(s) 67
SacI GAGCTC 1 cut(s) 363
SaqAI TTAA 2 cut(s) 408, 1109
SatI GCNGC 1 cut(s) 720
Sau3AI GATC 8 cut(s) 204, 526, 550, 610, 863, 900, 982, 1168
Sau96I GGNCC 1 cut(s) 1268
SchI GAGTC 1 cut(s) 502
ScrFI CCNGG 1 cut(s) 767
SduI GDGCHC 1 cut(s) 363
SfaNI GCATC 1 cut(s) 309
SfcI CTRYAG 1 cut(s) 960
SfuI TTCGAA 2 cut(s) 577, 1151
SmiMI CAYNNNNRTG 1 cut(s) 67
SmlI CTYRAG 3 cut(s) 328, 644, 1300
SmoI CTYRAG 3 cut(s) 328, 644, 1300
SphI GCATGC 1 cut(s) 66
SseBI AGGCCT 1 cut(s) 1014
SsiI CCGC 3 cut(s) 291, 495, 683
SspMI CTAG 3 cut(s) 324, 854, 956
SstI GAGCTC 1 cut(s) 363
StuI AGGCCT 1 cut(s) 1014
StyD4I CCNGG 1 cut(s) 765
TaaI ACNGT 2 cut(s) 20, 1136
TaiI ACGT 1 cut(s) 9
TaqI TCGA 9 cut(s) 381, 577, 1026, 1047, 1092, 1151, 1171, 1273, 1345
TaqII GACCGA 1 cut(s) 179
TatI WGTACW 1 cut(s) 808
TfiI GAWTC 8 cut(s) 426, 574, 579, 592, 838, 1018, 1153, 1259
Tru1I TTAA 2 cut(s) 408, 1109
Tru9I TTAA 2 cut(s) 408, 1109
TseFI GTSAC 3 cut(s) 85, 696, 1198
TseI GCWGC 1 cut(s) 719
Tsp45I GTSAC 3 cut(s) 85, 696, 1198
TspDTI ATGAA 5 cut(s) 154, 689, 693, 830, 968
TspGWI ACGGA 3 cut(s) 585, 715, 975
XapI RAATTY 4 cut(s) 25, 52, 775, 879
XceI RCATGY 1 cut(s) 66
XmnI GAANNNNTTC 1 cut(s) 56
XspI CTAG 3 cut(s) 324, 854, 956
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.