Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr10

Orthogroup ID

OG0016785

Physical Location & Seq

Genomic Coordinates Forward (+)

22970772 .. 22974245

Gene Structure 3474 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2505 bp

ATGGCAGGATCGTCTGAAAGAACAAAGAGGAAGATAAGGTGGAGCAAACTGTATTCTTTTGCATGCTTCAGACCTGTCACAACTGAGAATGATCCAAGCCAGCAGCTCCTTGGCCAGCCAGGGTTTTCGCGGGTGGTTTTCTGCAACGAGCCTCAGCTGCACAAGGCCAAGCCTTACAAGTACCCCAAGAACTATGTCTCCACAACCAAGTACAATGTTATGACTTTTCTTCCAAAAGCGCTTTTCGAGCAGTTCCGAAGAGTTGCCAATCTGTATTTTCTGTTGGCAGCTGTGTTGTCCGTCACTTCTTTGGCTCCTTTTCAACCAATAAGTTTGATTGCTCCTTTGGTCTTTGTGGTGGGGGTTAGCATGATCAAGGAGGCAGTGGAGGACTGGCACAGATTCTTGCAGGATTTGAATGTGAACTCAAGAATTGTAAAGGTTCACGTTGGAGACGGCGAATTTATTGAAAAACCATGGCAAGGACTGTGTGTAGGAGATGTTGTGAAGGTTAACAAGAATGAATATTTTCCTAGTGATCTCCTCTTACTATCTTCCAGCTATGAGGATGGCATATGTTATGTAGAGACCATGAACCTTGATGGAGAAACTAATCTCAAGGTTAAACGATGCTCGGAAGCCACACTTGGCCTTGTCAATGATCAATCATTTGGTTTATTCAGGGCTACCGTCTGTTGTGAGGACCCGAATCCTCATCTCTACACCTTTGTGGGGAACTTAGAGTTGAAAAATGCATCATTTCCCTTGTGCCCTGCCTCACTTCTCCTAAGAGATTCAAAGCTTCGGAACACAGATTATATTTATGGGGTTGTGATTTTTAGTGGACCAGACACAAAAGCGGTGAGGAACTCAACAAGATCACCATCTAAGCGCAGCCGGATTGAAAGAAAGATGGATCTTGTCATCTACCTTCTCTTTTCGATGCTTCTGTTGATTTCACTGGTCACTTCGATTGGTTTTGCAGAGTTTTTGAAAACGGAGATGATTAAGTGGTGGTATCTTTCTCTAGATGATAATGATCCATTTTTTCAACCACCAAGGCCTGAGGTATCTGGTTTCCTACAATTCATAAGGGCTCTTATTTTGTATGGTTACTTGATCCCCATTTCTCTCTATGTTTCCATTGAGGTTGTAAAAGTTTTACAAGCCATGCTCATTAACAAGGACATCAAATTGTATGATGAAGTTACATGTAAATCAGTTCAAACGCGAACGTCAAATTTAAATGAAGAGCTTGGTCAGGTGGAGATGATTCTGTCTGATAAAACGGGGACTTTGACGTGTAACCAGATGGAATTCAGGAAATGCTGTATTGCTGGAATTTCGTATGGGGGTGATGTAAATGAAATCGATCGTGCAGCATCTAAACGGATGAATGTTGATGTTGAGTCATATTGTTTTAGTATTGATGAGTTTGAAACAGCAAGCCAAAGCTGCGAAATGTTTGAGTTCTCAGTTGGTGACATTAGCACCGAAAGGGCTATTCTAGGTGGACAAAGACATGAGCAGAATTATTCAAGTGCAGAAAACTCGAGAATCTCTAATGTGGAGGAAGAATTTGCCATCAAGGGTTTCAACTTTAGGGATGGCAGGCTTATGAACAAAAAGTGGATGTACAGATCCAACTTATCCGATGTAACAATGTTCTTTCGAGTCATGGCTCTATGTCACACAGGCATACCTGTTGAGGAGGATGAGCTAACTCATAAGCTAAAGTATGAGGCAGAGTCACCAGAAGAAGTATCTTTTCTAGTTGCTGCACAAGAGTTTGGATTTCAATTTTTTCGAAGAAGTCAGTCCAGTATGTTTCTTAAGGAATTTGATCCTCCCATGGGAAAGGAGGTTGAAAGGAAGTACAAACTTTTGAACCTTTTAGAGTTCTGTAGCGCTCGGAAGAGGATGTCTGTTATAGTAAGCGACGAAGATGGTCAAATCATTCTACTCTGCAAAGGTGCAGACAACATTATCTTTGACAGGCTTGCAGAAAATGGAAGGTCATACCAACAAGCAACGACTCTACATCTTTCCAATTATGCAGAAGATGGATTCCGCACTCTGGCATTTGCTTACCGGAAACTTGAGGCTGCTGAGTATGAACAATGGAACTCAATATTCAAGGTGGCCAAGACAACAATAGGTCCCGAAAGGGAAGAAGTGCTAGAGAAAGCATCTGAAATGATCGAAAAGGATTTGATTTTATTAGGGGTTGCTGCTGTTGAAGACAAGCTACAAAAGGGGGTTCCAGAATGCATAGATAAACTTGCACAAGCCGGGATAAAAATATGGCTGCTCACCGGTGACAAGAAGGAAACTGCAATAAATATAGGATTTGCTTGCAGCTTACTCCGGCAGGATATGAAGCAATTTCATCTGAGTTTGGGTAGAGAAACTGCAACTATTCCAACTAATAACCAACCGAAGTTTCCAGGTGATGAAAGACGACATCTTAAACCAACTCGAAAGTTTTCACAAACTGAAGTCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004012 GO:0005215 GO:0005319 GO:0005548 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005794 GO:0005802 GO:0005886 GO:0006810 GO:0006811 GO:0006820 GO:0006869 GO:0006900 GO:0006996 GO:0008150 GO:0009987 GO:0010876 GO:0012505 GO:0015711 GO:0015748 GO:0015914 GO:0016020 GO:0016043 GO:0016050 GO:0016192 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0031984 GO:0033036 GO:0034204 GO:0042623 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044422 GO:0044424 GO:0044431 GO:0044444 GO:0044446 GO:0044464 GO:0045332 GO:0048193 GO:0048194 GO:0051179 GO:0051234 GO:0061024 GO:0065007 GO:0065008 GO:0071702 GO:0071840 GO:0071944 GO:0097035 GO:0098791

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Cation_ATPase E1-E2_ATPase Hydrolase PhoLip_ATPase_C PhoLip_ATPase_N

Protein Analysis

835

Amino Acids

95.1

Weight (kDa)

6.2

Isoelectric Point (pI)

43.32

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PhoLip_ATPase_N	PF16209	46 - 112	2e-23	Phospholipid-translocating ATPase N-terminal
Cation_ATPase	PF13246	555 - 752	1.3e-18	P-type ATPase, cytoplasmic domain N

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0016785)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_3g11830
malus_domestica	MD10G1238400.v1.1
prunus_persica	Prupe.4G105900_v2.0.a1
pyrus_communis	pycom10g19940
rosa_chinensis	RchiOBHm_Chr5g0019261
rosa_laevigata	RLG00000032432
rosa_multiflora	Rmu_co8517133.1_g000001
rosa_roxburghii	Rroxscaffold_1G00058740
rosa_rugosa	Rorug05G0049000
rosa_samantha	Rh5AG140100 Rh5BG139100 Rh5CG150500 Rh5DG139700
rosa_wichuraiana	Rw5G012460

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccII	CGCG	2 cut(s)	130, 1231
AciI	CCGC	3 cut(s)	130, 860, 2071
AclWI	GGATC	7 cut(s)	16, 86, 924, 1034, 1114, 1635, 1838
AcoI	YGGCCR	2 cut(s)	112, 2142
AcsI	RAATTY	6 cut(s)	461, 1240, 1316, 1341, 1577, 1838
AcuI	CTGAAG	1 cut(s)	52
AfaI	GTAC	4 cut(s)	182, 212, 1637, 1877
AfeI	AGCGCT	2 cut(s)	240, 1909
AfiI	CCNNNNNNNGG	4 cut(s)	482, 732, 1853, 2077
AflII	CTTAAG	1 cut(s)	1832
AflIII	ACRYGT	2 cut(s)	1211, 1301
AgeI	ACCGGT	1 cut(s)	2315
AjiI	CACGTC	1 cut(s)	1302
AjnI	CCWGG	2 cut(s)	118, 2446
AjuI	GAANNNNNNNTTGG	4 cut(s)	630, 662, 2427, 2459
AleI	CACNNNNGTG	1 cut(s)	728
AloI	GAACNNNNNNTCC	2 cut(s)	182, 214
Alw26I	GTCTC	3 cut(s)	202, 447, 581
AlwI	GGATC	7 cut(s)	16, 86, 924, 1034, 1114, 1635, 1838
Ama87I	CYCGRG	1 cut(s)	1552
Aor51HI	AGCGCT	2 cut(s)	240, 1909
AoxI	GGCC	5 cut(s)	112, 165, 649, 1061, 2142
ApoI	RAATTY	6 cut(s)	461, 1240, 1316, 1341, 1577, 1838
AsiGI	ACCGGT	1 cut(s)	2315
Asp700I	GAANNNNTTC	2 cut(s)	528, 2485
AspLEI	GCGC	3 cut(s)	241, 894, 1910
AspS9I	GGNCC	3 cut(s)	703, 845, 2159
AsuC2I	CCSGG	1 cut(s)	2293
AsuHPI	GGTGA	8 cut(s)	873, 874, 1367, 1493, 1743, 2305, 2330, 2462
AsuII	TTCGAA	1 cut(s)	1807
AvaI	CYCGRG	1 cut(s)	1552
AvaII	GGWCC	3 cut(s)	703, 845, 2159
AxyI	CCTNAGG	1 cut(s)	1065
BaeGI	GKGCMC	1 cut(s)	773
BalI	TGGCCA	2 cut(s)	114, 2144
BanII	GRGCYC	1 cut(s)	1099
BarI	GAAGNNNNNNTAC	2 cut(s)	2232, 2264
BbsI	GAAGAC	1 cut(s)	2247
BbvCI	CCTCAGC	1 cut(s)	153
BccI	CCATC	9 cut(s)	563, 596, 892, 907, 1306, 1592, 1601, 1940, 2057
BceAI	ACGGC	1 cut(s)	472
BcgI	CGANNNNNNTGC	2 cut(s)	1533, 1567
BciT130I	CCWGG	2 cut(s)	120, 2448
BclI	TGATCA	2 cut(s)	372, 661
BcnI	CCSGG	1 cut(s)	2293
BcoDI	GTCTC	3 cut(s)	202, 447, 581
BfaI	CTAG	5 cut(s)	534, 1028, 1508, 1772, 2180
BfmI	CTRYAG	1 cut(s)	1903
BfoI	RGCGCY	2 cut(s)	242, 1911
BfrI	CTTAAG	1 cut(s)	1832
Bme1390I	CCNGG	3 cut(s)	120, 2293, 2448
Bme18I	GGWCC	3 cut(s)	703, 845, 2159
BmeT110I	CYCGRG	1 cut(s)	1552
BmgBI	CACGTC	1 cut(s)	1302
BmgT120I	GGNCC	3 cut(s)	703, 845, 2159
BmiI	GGNNCC	4 cut(s)	315, 705, 2161, 2262
BmrFI	CCNGG	3 cut(s)	120, 2293, 2448
BmsI	GCATC	5 cut(s)	620, 764, 933, 1391, 2198
BpiI	GAAGAC	1 cut(s)	2247
Bpu10I	CCTNAGC	1 cut(s)	153
Bpu14I	TTCGAA	1 cut(s)	1807
BpuEI	CTTGAG	3 cut(s)	412, 602, 2120
BpuMI	CCSGG	1 cut(s)	2293
Bsa29I	ATCGAT	1 cut(s)	1371
BsaBI	GATNNNNATC	2 cut(s)	883, 1038
BsaI	GGTCTC	1 cut(s)	581
BsaJI	CCNNGG	5 cut(s)	109, 119, 476, 1058, 1851
BsaWI	WCCGGW	2 cut(s)	2091, 2315
BsaXI	ACNNNNNCTCC	4 cut(s)	182, 212, 489, 519
Bsc4I	CCNNNNNNNGG	4 cut(s)	482, 732, 1853, 2077
Bse118I	RCCGGY	1 cut(s)	2315
Bse1I	ACTGG	3 cut(s)	398, 966, 1821
Bse21I	CCTNAGG	1 cut(s)	1065
Bse8I	GATNNNNATC	2 cut(s)	883, 1038
BseBI	CCWGG	2 cut(s)	120, 2448
BseCI	ATCGAT	1 cut(s)	1371
BseDI	CCNNGG	5 cut(s)	109, 119, 476, 1058, 1851
BseGI	GGATG	6 cut(s)	574, 1398, 1612, 1638, 1720, 1926
BseJI	GATNNNNATC	2 cut(s)	883, 1038
BseLI	CCNNNNNNNGG	4 cut(s)	482, 732, 1853, 2077
BseMII	CTCAG	6 cut(s)	75, 167, 1056, 1488, 2100, 2384
BseNI	ACTGG	3 cut(s)	398, 966, 1821
BseRI	GAGGAG	2 cut(s)	533, 1724
BseSI	GKGCMC	1 cut(s)	773
BsgI	GTGCAG	5 cut(s)	143, 1398, 1563, 1764, 1995
Bsh1236I	CGCG	2 cut(s)	130, 1231
Bsh1285I	CGRYCG	1 cut(s)	1375
BshFI	GGCC	5 cut(s)	114, 167, 651, 1063, 2144
BshTI	ACCGGT	1 cut(s)	2315
BshVI	ATCGAT	1 cut(s)	1371
BsiEI	CGRYCG	1 cut(s)	1375
BsiHKCI	CYCGRG	1 cut(s)	1552
BsiSI	CCGG	5 cut(s)	898, 2092, 2292, 2316, 2368
BslFI	GGGAC	2 cut(s)	1306, 2145
BslI	CCNNNNNNNGG	4 cut(s)	482, 732, 1853, 2077
BsmAI	GTCTC	3 cut(s)	202, 447, 581
BsmBI	CGTCTC	1 cut(s)	447
BsmFI	GGGAC	2 cut(s)	1306, 2145
BsmI	GAATGC	1 cut(s)	2273
BsnI	GGCC	5 cut(s)	114, 167, 651, 1063, 2144
Bso31I	GGTCTC	1 cut(s)	581
BsoBI	CYCGRG	1 cut(s)	1552
Bsp119I	TTCGAA	1 cut(s)	1807
Bsp1286I	GDGCHC	2 cut(s)	773, 1099
Bsp1407I	TGTACA	1 cut(s)	1635
Bsp19I	CCATGG	2 cut(s)	476, 1851
BspACI	CCGC	3 cut(s)	130, 860, 2071
BspANI	GGCC	5 cut(s)	114, 167, 651, 1063, 2144
BspCNI	CTCAG	6 cut(s)	76, 166, 1057, 1487, 2101, 2385
BspDI	ATCGAT	1 cut(s)	1371
BspFNI	CGCG	2 cut(s)	130, 1231
BspLI	GGNNCC	4 cut(s)	315, 705, 2161, 2262
BspPI	GGATC	7 cut(s)	16, 86, 924, 1034, 1114, 1635, 1838
BspQI	GCTCTTC	1 cut(s)	1245
BspT104I	TTCGAA	1 cut(s)	1807
BspTI	CTTAAG	1 cut(s)	1832
BspTNI	GGTCTC	1 cut(s)	581
BsrFI	RCCGGY	1 cut(s)	2315
BsrGI	TGTACA	1 cut(s)	1635
BsrI	ACTGG	3 cut(s)	398, 966, 1821
BssAI	RCCGGY	1 cut(s)	2315
BssECI	CCNNGG	5 cut(s)	109, 119, 476, 1058, 1851
BssT1I	CCWWGG	4 cut(s)	109, 476, 1058, 1851
Bst2UI	CCWGG	2 cut(s)	120, 2448
Bst4CI	ACNGT	3 cut(s)	51, 489, 691
Bst6I	CTCTTC	3 cut(s)	253, 1245, 1910
BstAFI	CTTAAG	1 cut(s)	1832
BstAUI	TGTACA	1 cut(s)	1635
BstBI	TTCGAA	1 cut(s)	1807
BstC8I	GCNNGC	7 cut(s)	64, 101, 116, 1447, 1613, 2001, 2356
BstDEI	CTNAG	9 cut(s)	84, 153, 739, 788, 888, 1065, 1474, 2109, 2393
BstDSI	CCRYGG	2 cut(s)	476, 1851
BstF5I	GGATG	6 cut(s)	574, 1398, 1612, 1638, 1720, 1926
BstFNI	CGCG	2 cut(s)	130, 1231
BstH2I	RGCGCY	2 cut(s)	242, 1911
BstHHI	GCGC	3 cut(s)	241, 894, 1910
BstMAI	GTCTC	3 cut(s)	202, 447, 581
BstMCI	CGRYCG	1 cut(s)	1375
BstMWI	GCNNNNNNNGC	3 cut(s)	157, 247, 1455
BstNI	CCWGG	2 cut(s)	120, 2448
BstNSI	RCATGY	2 cut(s)	66, 1215
BstSCI	CCNGG	3 cut(s)	118, 2291, 2446
BstSFI	CTRYAG	1 cut(s)	1903
BstSLI	GKGCMC	1 cut(s)	773
BstUI	CGCG	2 cut(s)	130, 1231
BstV2I	GAAGAC	1 cut(s)	2247
BstX2I	RGATCY	2 cut(s)	916, 1640
BstYI	RGATCY	2 cut(s)	916, 1640
Bsu15I	ATCGAT	1 cut(s)	1371
Bsu36I	CCTNAGG	1 cut(s)	1065
BsuRI	GGCC	5 cut(s)	114, 167, 651, 1063, 2144
BsuTUI	ATCGAT	1 cut(s)	1371
BtgI	CCRYGG	2 cut(s)	476, 1851
BtrI	CACGTC	1 cut(s)	1302
BtsCI	GGATG	6 cut(s)	574, 1398, 1612, 1638, 1720, 1926
BtsI	GCAGTG	1 cut(s)	390
BtsIMutI	CAGTG	2 cut(s)	390, 959
Cac8I	GCNNGC	7 cut(s)	64, 101, 116, 1447, 1613, 2001, 2356
CfoI	GCGC	3 cut(s)	241, 894, 1910
Cfr10I	RCCGGY	1 cut(s)	2315
Cfr13I	GGNCC	3 cut(s)	703, 845, 2159
ClaI	ATCGAT	1 cut(s)	1371
Csp6I	GTAC	4 cut(s)	181, 211, 1636, 1876
CspAI	ACCGGT	1 cut(s)	2315
CviAII	CATG	9 cut(s)	63, 370, 477, 592, 1171, 1212, 1523, 1678, 1852
CviQI	GTAC	4 cut(s)	181, 211, 1636, 1876
DdeI	CTNAG	9 cut(s)	84, 153, 739, 788, 888, 1065, 1474, 2109, 2393
DraI	TTTAAA	1 cut(s)	1245
EaeI	YGGCCR	2 cut(s)	112, 2142
Eam1104I	CTCTTC	3 cut(s)	253, 1245, 1910
EarI	CTCTTC	3 cut(s)	253, 1245, 1910
Eco130I	CCWWGG	4 cut(s)	109, 476, 1058, 1851
Eco147I	AGGCCT	1 cut(s)	1063
Eco24I	GRGCYC	1 cut(s)	1099
Eco31I	GGTCTC	1 cut(s)	581
Eco47I	GGWCC	3 cut(s)	703, 845, 2159
Eco47III	AGCGCT	2 cut(s)	240, 1909
Eco57I	CTGAAG	1 cut(s)	52
Eco81I	CCTNAGG	1 cut(s)	1065
Eco88I	CYCGRG	1 cut(s)	1552
EcoO109I	RGGNCCY	2 cut(s)	703, 2159
EcoRI	GAATTC	1 cut(s)	1316
EcoRII	CCWGG	2 cut(s)	118, 2446
EcoT14I	CCWWGG	4 cut(s)	109, 476, 1058, 1851
EcoT22I	ATGCAT	2 cut(s)	757, 2273
EcoT38I	GRGCYC	1 cut(s)	1099
ErhI	CCWWGG	4 cut(s)	109, 476, 1058, 1851
Esp3I	CGTCTC	1 cut(s)	447
FaeI	CATG	9 cut(s)	66, 373, 480, 595, 1174, 1215, 1526, 1681, 1855
FalI	AAGNNNNNCTT	2 cut(s)	630, 662
FaqI	GGGAC	2 cut(s)	1306, 2145
FatI	CATG	9 cut(s)	62, 369, 476, 591, 1170, 1211, 1522, 1677, 1851
FauI	CCCGC	1 cut(s)	123
FauNDI	CATATG	1 cut(s)	575
FbaI	TGATCA	2 cut(s)	372, 661
FokI	GGATG	6 cut(s)	581, 1405, 1619, 1645, 1727, 1933
FriOI	GRGCYC	1 cut(s)	1099
FspBI	CTAG	5 cut(s)	534, 1028, 1508, 1772, 2180
GlaI	GCGC	3 cut(s)	240, 893, 1909
HaeII	RGCGCY	2 cut(s)	242, 1911
HaeIII	GGCC	5 cut(s)	114, 167, 651, 1063, 2144
HapII	CCGG	5 cut(s)	898, 2092, 2292, 2316, 2368
HhaI	GCGC	3 cut(s)	241, 894, 1910
Hin1II	CATG	9 cut(s)	66, 373, 480, 595, 1174, 1215, 1526, 1681, 1855
Hin6I	GCGC	3 cut(s)	239, 892, 1908
HinP1I	GCGC	3 cut(s)	239, 892, 1908
HincII	GTYRAC	1 cut(s)	514
HindII	GTYRAC	1 cut(s)	514
HindIII	AAGCTT	1 cut(s)	800
HpaI	GTTAAC	1 cut(s)	514
HpaII	CCGG	5 cut(s)	898, 2092, 2292, 2316, 2368
HphI	GGTGA	8 cut(s)	873, 874, 1367, 1493, 1743, 2305, 2330, 2462
Hpy166II	GTNNAC	5 cut(s)	424, 445, 514, 845, 1514
Hpy188III	TCNNGA	6 cut(s)	429, 1028, 1321, 1554, 2162, 2264
Hpy8I	GTNNAC	5 cut(s)	424, 445, 514, 845, 1514
Hpy99I	CGWCG	1 cut(s)	1943
HpyAV	CCTTC	4 cut(s)	502, 941, 2007, 2320
HpyCH4III	ACNGT	3 cut(s)	51, 489, 691
HpyCH4IV	ACGT	3 cut(s)	447, 1235, 1301
HpyF10VI	GCNNNNNNNGC	3 cut(s)	157, 247, 1455
HpyF3I	CTNAG	9 cut(s)	84, 153, 739, 788, 888, 1065, 1474, 2109, 2393
HpySE526I	ACGT	3 cut(s)	447, 1235, 1301
Hsp92II	CATG	9 cut(s)	66, 373, 480, 595, 1174, 1215, 1526, 1681, 1855
HspAI	GCGC	3 cut(s)	239, 892, 1908
Ksp22I	TGATCA	2 cut(s)	372, 661
KspAI	GTTAAC	1 cut(s)	514
LguI	GCTCTTC	1 cut(s)	1245
LmnI	GCTCC	4 cut(s)	42, 111, 319, 346
LweI	GCATC	5 cut(s)	620, 764, 933, 1391, 2198
MaeI	CTAG	5 cut(s)	534, 1028, 1508, 1772, 2180
MaeII	ACGT	3 cut(s)	447, 1235, 1301
MflI	RGATCY	2 cut(s)	916, 1640
MhlI	GDGCHC	2 cut(s)	773, 1099
MlsI	TGGCCA	2 cut(s)	114, 2144
MluNI	TGGCCA	2 cut(s)	114, 2144
MlyI	GAGTC	4 cut(s)	1418, 1683, 1757, 2029
MmeI	TCCRAC	3 cut(s)	430, 1668, 2447
Mox20I	TGGCCA	2 cut(s)	114, 2144
Mph1103I	ATGCAT	2 cut(s)	757, 2273
MroXI	GAANNNNTTC	2 cut(s)	528, 2485
MscI	TGGCCA	2 cut(s)	114, 2144
MseI	TTAA	7 cut(s)	513, 624, 1008, 1179, 1244, 1833, 2469
MslI	CAYNNNNRTG	1 cut(s)	728
Msp20I	TGGCCA	2 cut(s)	114, 2144
MspA1I	CMGCKG	2 cut(s)	157, 290
MspCI	CTTAAG	1 cut(s)	1832
MspI	CCGG	5 cut(s)	898, 2092, 2292, 2316, 2368
MspR9I	CCNGG	3 cut(s)	120, 2293, 2448
Mva1269I	GAATGC	1 cut(s)	2273
MvaI	CCWGG	2 cut(s)	120, 2448
MvnI	CGCG	2 cut(s)	130, 1231
MwoI	GCNNNNNNNGC	3 cut(s)	157, 247, 1455
NciI	CCSGG	1 cut(s)	2293
NcoI	CCATGG	2 cut(s)	476, 1851
NdeI	CATATG	1 cut(s)	575
NlaIII	CATG	9 cut(s)	66, 373, 480, 595, 1174, 1215, 1526, 1681, 1855
NlaIV	GGNNCC	4 cut(s)	315, 705, 2161, 2262
NmuCI	GTSAC	7 cut(s)	76, 301, 964, 1481, 1688, 1749, 2318
NsiI	ATGCAT	2 cut(s)	757, 2273
NspI	RCATGY	2 cut(s)	66, 1215
NspV	TTCGAA	1 cut(s)	1807
OliI	CACNNNNGTG	1 cut(s)	728
PaeI	GCATGC	1 cut(s)	66
PaeR7I	CTCGAG	1 cut(s)	1552
PceI	AGGCCT	1 cut(s)	1063
PciI	ACATGT	1 cut(s)	1211
PciSI	GCTCTTC	1 cut(s)	1245
PctI	GAATGC	1 cut(s)	2273
PdmI	GAANNNNTTC	2 cut(s)	528, 2485
PfeI	GAWTC	6 cut(s)	402, 709, 794, 1273, 1557, 2067
PinAI	ACCGGT	1 cut(s)	2315
Ple19I	CGATCG	1 cut(s)	1375
PleI	GAGTC	4 cut(s)	1417, 1682, 1756, 2029
PpsI	GAGTC	4 cut(s)	1417, 1682, 1756, 2029
PpuMI	RGGWCCY	2 cut(s)	703, 2159
PscI	ACATGT	1 cut(s)	1211
Psp5II	RGGWCCY	2 cut(s)	703, 2159
Psp6I	CCWGG	2 cut(s)	118, 2446
PspGI	CCWGG	2 cut(s)	118, 2446
PspN4I	GGNNCC	4 cut(s)	315, 705, 2161, 2262
PspPI	GGNCC	3 cut(s)	703, 845, 2159
PspPPI	RGGWCCY	2 cut(s)	703, 2159
PsuI	RGATCY	2 cut(s)	916, 1640
PvuI	CGATCG	1 cut(s)	1375
PvuII	CAGCTG	2 cut(s)	157, 290
RsaI	GTAC	4 cut(s)	182, 212, 1637, 1877
RsaNI	GTAC	4 cut(s)	181, 211, 1636, 1876
RseI	CAYNNNNRTG	1 cut(s)	728
SapI	GCTCTTC	1 cut(s)	1245
SaqAI	TTAA	7 cut(s)	513, 624, 1008, 1179, 1244, 1833, 2469
Sau96I	GGNCC	3 cut(s)	703, 845, 2159
SchI	GAGTC	4 cut(s)	1418, 1683, 1757, 2029
ScrFI	CCNGG	3 cut(s)	120, 2293, 2448
SduI	GDGCHC	2 cut(s)	773, 1099
SfaNI	GCATC	5 cut(s)	620, 764, 933, 1391, 2198
SfcI	CTRYAG	1 cut(s)	1903
Sfr274I	CTCGAG	1 cut(s)	1552
SfuI	TTCGAA	1 cut(s)	1807
SgrAI	CRCCGGYG	1 cut(s)	2315
SinI	GGWCC	3 cut(s)	703, 845, 2159
SlaI	CTCGAG	1 cut(s)	1552
SmiI	ATTTAAAT	1 cut(s)	1245
SmiMI	CAYNNNNRTG	1 cut(s)	728
SmlI	CTYRAG	5 cut(s)	427, 617, 1552, 1832, 2099
SmoI	CTYRAG	5 cut(s)	427, 617, 1552, 1832, 2099
SphI	GCATGC	1 cut(s)	66
SseBI	AGGCCT	1 cut(s)	1063
SsiI	CCGC	3 cut(s)	130, 860, 2071
SspI	AATATT	2 cut(s)	527, 2133
SspMI	CTAG	5 cut(s)	534, 1028, 1508, 1772, 2180
StuI	AGGCCT	1 cut(s)	1063
StyD4I	CCNGG	3 cut(s)	118, 2291, 2446
StyI	CCWWGG	4 cut(s)	109, 476, 1058, 1851
SwaI	ATTTAAAT	1 cut(s)	1245
TaaI	ACNGT	3 cut(s)	51, 489, 691
TaiI	ACGT	3 cut(s)	450, 1238, 1304
TaqI	TCGA	9 cut(s)	246, 941, 971, 1371, 1553, 1672, 1807, 2202, 2479
TatI	WGTACW	3 cut(s)	210, 1635, 1875
TfiI	GAWTC	6 cut(s)	402, 709, 794, 1273, 1557, 2067
Tru1I	TTAA	7 cut(s)	513, 624, 1008, 1179, 1244, 1833, 2469
Tru9I	TTAA	7 cut(s)	513, 624, 1008, 1179, 1244, 1833, 2469
TscAI	CASTG	2 cut(s)	390, 966
TseFI	GTSAC	7 cut(s)	76, 301, 964, 1481, 1688, 1749, 2318
Tsp45I	GTSAC	7 cut(s)	76, 301, 964, 1481, 1688, 1749, 2318
TspGWI	ACGGA	3 cut(s)	289, 1013, 1405
TspRI	CASTG	2 cut(s)	390, 966
Vha464I	CTTAAG	1 cut(s)	1832
VpaK11BI	GGWCC	3 cut(s)	703, 845, 2159
XapI	RAATTY	6 cut(s)	461, 1240, 1316, 1341, 1577, 1838
XbaI	TCTAGA	1 cut(s)	1027
XceI	RCATGY	2 cut(s)	66, 1215
XcmI	CCANNNNNNNNNTGG	1 cut(s)	107
XhoI	CTCGAG	1 cut(s)	1552
XmnI	GAANNNNTTC	2 cut(s)	528, 2485
XspI	CTAG	5 cut(s)	534, 1028, 1508, 1772, 2180
Zsp2I	ATGCAT	2 cut(s)	757, 2273

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.