Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr10

Orthogroup ID

OG0009295

Physical Location & Seq

Genomic Coordinates Reverse (-)

23828988 .. 23837631

Gene Structure 8644 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3207 bp

ATGAGCGTATGGAACTACGTCGTAACGGCTCACAAGCCCACCAACGTCACACACTCCTGCGTCGGCAACTTCACCGCCCCTCAGGAGCTCAATCTCATCACTGCGAAATGTACGCGAATTGAAATTCAGCTACTTACTCCTCTGGGACTGCAGCCTATCTTGGATGTTCCAATATATGGAAGAATAGCGACGCTTGAACTTTTCCGGCCTCATGGTGAAACACAGGATTTTCTATTTATTGCAACAGAAAGGTACAAATTTTGTGTTCTTCAGTGGGATGCCGAAGCATCTGAGCTCATCACAAGGGCAATGGGGGATGTCTCAGATCGCATAGGTCGTCCTACAGACAATGGTCAAGTTATTCCTTTTGATAATAAAGGGCAGCTCAAGGAAGCATTTAATATACGGCTTGAGGAACTTCAAGTTTTGGATATCAAGTTTCTATATGGTTGTTCAAAACCAACAATTGTAGTTCTATATCAGGATAATAAAGATGTTCGCCATGTCAAAACATATGAGGTTTCTCTGAAGGATAAGGATTTTGTCGAAGGTCCTTGGGCACAGAACAACGTTGAAAATGGGGCTGATTTGTTAATACCGGTTCCTCCGCCACTCTGTGGTGTTCTTATTATTGGAGAAGATAATATTGTTTACCGCAGCCCGAATACATTTAAAGCAATCCCAATAAGACATTCCATCACAAGAGCCTATGGGAGAGTAGATGCAGATGGTTCCAGGTATTTACTTGGTGATCATGCCGGGCTACTTCACCTACTTGTTATAACGCATGAGAAAGAAAAGGTGACTGGACTGAAAATTGAGCTCTTGGGGGAGACATCAATTGCATCTACTATTTCATACCTTGATAATGCATTTGTCTATATCGGATCAAGTTATGGGGATTCACAGCTTGTAAAGCTAAATCTACAGCCTGATGCAAAGGGTTCATATGTGGAAGTCTTAGAAAGGTATGTCAATTTGGGGCCGATCGTTGACTTTTGTGTGGTCGATCTTGAGAGGCAAGGCCAAGGTCAGGTTGTGACTTGTTCTGGAGCTTTCAAGGATGGTTCTCTTCGTGTAGTTCGGAATGGAATTGGAATCAATGAACAGGCATCAGTTGAGCTTCAAGGTATCAAGGGAATGTGGTCATTAAGATCCTCCACTGATGATCCTTTTGACACCTTCCTGGTTGTAAGCTTTATCAGTGAGACAAGGATTTTGGCAATGAATCTTGAGGATGAGTTGGAAGAAACTGAGATAGAAGGCTTCTGTTCTCAAGTTCAGACTTTATTTTGCCACGATGCCGTCTGTAATCAACTGGTACAGGTCACTTCAAGCTCTGTACGACTAGTCAGTTCTACAACCAGGGAGCTCAAGCATGAGTGGAATGCTCCAGCAGGCAATTCGATTAATGTTGCCACTGCTAATGCCACCCAGGTTTTATTGGCGACTGGTGGAAAGCATTTAGTTTATTTGGAAATAGGTGATGGGGTATTGACAGAGAAAAACCACGCACAGTTAGATTTTGAGATATCATGCCTTGACATAAACCCCATAGGCGACAATCCCAACTATAGCCAGCTGGCAGCTGTTGGAATGTGGACAGATATTAGTGTCCAGATATATTCACTTCCTGACCTGAGCCGCATCACAAAGGAACATTTGGCATGGGAGATCATACCTCGTTCTGTTCTTCTTTGTGCCTTTGAAGGGATATCCTACCTATTGTGTGCCCTTGGAGATGGGCACCTCTTAAACTTTATTTTGAACACAAGCACTGGTGAGTTGACGGACAGGAAAAAAGTCTCACTTGGGACGCAACCCATTACACTCCGTACCTTCTCATCCAAGAGTACTACGCATGTATTTGCTGCATCGGATAGGCCAACTGTCATCTATAGTAGCAACAAGAAATTGCTTTACAGCAACGTAAATCTGAAAGAAGTCAGCCATATGTGCCCATTCAACTCTGCTGCTTTTCCAGACAGCCTTGCAATTGCTAAGGAAGGTGAACTTACTATTGGCACAATTGATGACATCCAGAAGCTTCATATTCGCTCTATTCCCCTAGGCGAACATGCACGACGTATCTGTCATCAGGAGCAGTCCAGGACTTTCGCCATTTTGAGTGTGAAATATAACCAGTCTAGTGCAGAAGACTCAGAAACACACTTCATCCGATTGTTGGATGACCAGAGTTTTGACCTCATAACATTTTATCAACTGGACACTTTTGAGTATGCCTGCTCTATACTTAGCTGTTCTTTCTCAGATGACAGTAATTTATATTACTGTGTCGGGACTGCATATGTTCTCCCGGAAGAAAATGAGCCAACCAAGGGCCGCATTCTGGTTTTCATTGTTGAAGATGGGAAGCTGCAGCTCGTTGCTGAGAAGGAAACCAAGGGGTCTGTTTACTCGTTGAATGCGTTCAATGGCAAGTTACTTGCTGCTATTAATCAGAAGATACAGTTATACAAGTGGACTCTGCGGGACGATGGTACTCGTGAATTGCAATCGGAATGTGGACACCATGGCCACATACTTGCCCTTTATGTCCAGACTCGTGGAGATTTCATCATTGTTGGGGACTTAATGAAGTCAATTTCTCTGTTAATGTACAAGCATGAGGAAGGTGCCATTGAGGAGCGAGCCCGTGATTATAATGCAAATTGGATGTCTGCTGTAGAGATCCTCGACGACGATGTTTACATTGGAGCTGAAAATTTCGTCAACCTGTTCACTGTTCGTAAAAATAGTGAGGGTGCAACTGATGAGGAACGGGGCCGCCTTGAGGTGGTAGGTGAGTATCACCTAGGCGAGTTTGTCAATAGGTTCCGGCACGGCTCTCTTGTCATGCGGTTGCCGGACTCTGACGTGGGCCAGATTCCAACTGTCATCTTTGGCACCGTCAATGGTGTAATTGGAGTAATTGCCTCACTTCCTCAAGAACAATATGCATTTTTGGAGAAGCTCCAGTCAAACTTGAGGAAGGTGATAAAGGGTGTTGGAGGGTTGAACCATGAGCAGTGGAGATCATTCAGCAACGAGAAGAAAACTGTAGACGCGAAAAATTTCTTGGATGGCGATCTGATAGAATCGTTCCTTGATCTCAACCGTAGCAAGATGGACGAGATCTCAAAACAAATGCGCGTTTCAGTGGAGGAGCTCTGCAAGAGAGTAGAAGAAATGACAAGGCTGCATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000151 GO:0003006 GO:0003674 GO:0003676 GO:0003677 GO:0003684 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006139 GO:0006259 GO:0006281 GO:0006289 GO:0006508 GO:0006511 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0007275 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009790 GO:0009791 GO:0009793 GO:0009987 GO:0010154 GO:0010498 GO:0019538 GO:0019941 GO:0022414 GO:0030163 GO:0031461 GO:0032501 GO:0032502 GO:0032991 GO:0033554 GO:0034641 GO:0043161 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0050896 GO:0051603 GO:0051716 GO:0061458 GO:0071704 GO:0080008 GO:0090304 GO:0097159 GO:1901360 GO:1901363 GO:1901564 GO:1901565 GO:1901575 GO:1902494 GO:1990234

KEGG Pathways

Metabolic & Signaling

ko03420 ko04120 ko05161 ko05203 map03420 map04120 map05161 map05203

Pfam Domains

Protein Families

CPSF_A MMS1_N

Protein Analysis

1069

Amino Acids

119.38

Weight (kDa)

5.2

Isoelectric Point (pI)

35.17

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Beta-prop_RSE1_1st	PF10433	16 - 128	2.3e-21	RSE1/DDB1/CPSF1 first beta-propeller
Beta-prop_RSE1_1st	PF10433	126 - 323	3.4e-41	RSE1/DDB1/CPSF1 first beta-propeller
Beta-prop_RSE1_2nd	PF23726	368 - 677	1.8e-67	RSE1/DDB1/CPSF1 second beta-propeller
CPSF_A	PF03178	724 - 1037	1.1e-81	CPSF A subunit region

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Publication-ready

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Orthologous Genes (Group: OG0009295)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G05420 AT4G05420 AT4G21100
fragaria_vesca	FvH4_3g09700 FvH4_3g09700
malus_domestica	MD00G1109600.v1.1 MD05G1276000.v1.1
prunus_persica	Prupe.4G088300_v2.0.a1 Prupe.4G088300_v2.0.a1
pyrus_communis	pycom05g25700 pycom10g21110
rosa_chinensis	RchiOBHm_Chr5g0015371
rosa_laevigata	RLG00000032196
rosa_multiflora	Rmu_sc0017755.1_g000009 Rmu_ssc0000085.1_g000001
rosa_roxburghii	Rroxscaffold_1G00061090
rosa_rugosa	Rorug05G0026900
rosa_samantha	Rh5AG120500 Rh5BG119300 Rh5CG129900 Rh5DG118500
rosa_wichuraiana	Rw5G010420

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	782, 2664
AasI	GACNNNNNNGTC	1 cut(s)	2091
AccB1I	GGYRCC	3 cut(s)	1746, 2636, 2906
AccB7I	CCANNNNNTGG	2 cut(s)	176, 617
AccI	GTMKAC	1 cut(s)	3063
AccII	CGCG	3 cut(s)	115, 3068, 3153
AciI	CCGC	8 cut(s)	75, 608, 655, 1645, 2344, 2491, 2788, 2860
AclI	AACGTT	1 cut(s)	570
AclWI	GGATC	4 cut(s)	895, 1149, 1163, 2686
AcoI	YGGCCR	1 cut(s)	2536
AcsI	RAATTY	4 cut(s)	123, 257, 2725, 3073
AcuI	CTGAAG	2 cut(s)	254, 548
AdeI	CACNNNGTG	1 cut(s)	617
AfaI	GTAC	8 cut(s)	112, 254, 1323, 1344, 1837, 1855, 2503, 2621
AfiI	CCNNNNNNNGG	9 cut(s)	176, 617, 1033, 1710, 2185, 2339, 2350, 2794, 2797
AgeI	ACCGGT	1 cut(s)	598
AhlI	ACTAGT	1 cut(s)	1348
AjiI	CACGTC	1 cut(s)	2878
AjnI	CCWGG	5 cut(s)	734, 1185, 1364, 1434, 2108
AjuI	GAANNNNNNNTTGG	6 cut(s)	676, 708, 2004, 2036, 3062, 3094
Alw21I	GWGCWC	5 cut(s)	90, 297, 825, 1374, 3171
Alw26I	GTCTC	4 cut(s)	325, 827, 1202, 1810
AlwI	GGATC	4 cut(s)	895, 1149, 1163, 2686
AoxI	GGCC	8 cut(s)	206, 983, 1024, 1883, 2341, 2536, 2785, 2881
ApoI	RAATTY	4 cut(s)	123, 257, 2725, 3073
ArsI	GACNNNNNNTTYG	6 cut(s)	1201, 1233, 2183, 2215, 2663, 2695
AseI	ATTAAT	2 cut(s)	1410, 2457
AsiGI	ACCGGT	1 cut(s)	598
Asp700I	GAANNNNTTC	2 cut(s)	2429, 3074
AspA2I	CCTAGG	2 cut(s)	2068, 2815
AspLEI	GCGC	1 cut(s)	3153
AspS9I	GGNCC	5 cut(s)	551, 983, 2341, 2785, 2881
AsuC2I	CCSGG	2 cut(s)	760, 2318
AvaII	GGWCC	1 cut(s)	551
AvrII	CCTAGG	2 cut(s)	2068, 2815
AxyI	CCTNAGG	1 cut(s)	81
BaeGI	GKGCMC	4 cut(s)	562, 1735, 1749, 1961
BalI	TGGCCA	1 cut(s)	2538
BanI	GGYRCC	3 cut(s)	1746, 2636, 2906
BanII	GRGCYC	6 cut(s)	90, 297, 825, 1374, 2656, 3171
BauI	CACGAG	2 cut(s)	2505, 2565
BbsI	GAAGAC	1 cut(s)	2163
Bbv12I	GWGCWC	5 cut(s)	90, 297, 825, 1374, 3171
BccI	CCATC	9 cut(s)	704, 722, 1058, 1481, 1736, 2363, 2492, 3077, 3121
BceAI	ACGGC	4 cut(s)	42, 422, 1289, 2860
BcgI	CGANNNNNNTGC	2 cut(s)	1386, 1420
BciT130I	CCWGG	5 cut(s)	736, 1187, 1366, 1436, 2110
BclI	TGATCA	1 cut(s)	751
BcnI	CCSGG	2 cut(s)	760, 2318
BcoDI	GTCTC	4 cut(s)	325, 827, 1202, 1810
BcuI	ACTAGT	1 cut(s)	1348
BfaI	CTAG	4 cut(s)	1349, 2069, 2148, 2816
BfmI	CTRYAG	8 cut(s)	149, 342, 926, 1573, 1897, 2378, 2685, 3060
BglII	AGATCT	1 cut(s)	3135
BlnI	CCTAGG	2 cut(s)	2068, 2815
BmcAI	AGTACT	1 cut(s)	1855
Bme1390I	CCNGG	7 cut(s)	736, 760, 1187, 1366, 1436, 2110, 2318
Bme18I	GGWCC	1 cut(s)	551
BmgBI	CACGTC	1 cut(s)	2878
BmgT120I	GGNCC	5 cut(s)	551, 983, 2341, 2785, 2881
BmiI	GGNNCC	8 cut(s)	603, 733, 984, 1748, 2638, 2786, 2837, 2908
BmrFI	CCNGG	7 cut(s)	736, 760, 1187, 1366, 1436, 2110, 2318
BmsI	GCATC	9 cut(s)	268, 296, 712, 854, 925, 1121, 1291, 1656, 1883
BoxI	GACNNNNGTC	1 cut(s)	351
BpiI	GAAGAC	1 cut(s)	2163
BpmI	CTGGAG	3 cut(s)	1071, 1377, 2960
Bpu10I	CCTNAGC	2 cut(s)	1640, 2001
BpuEI	CTTGAG	9 cut(s)	371, 431, 1034, 1253, 1260, 1358, 2813, 2931, 3007
BpuMI	CCSGG	2 cut(s)	760, 2318
BsaBI	GATNNNNATC	1 cut(s)	3087
BsaWI	WCCGGW	1 cut(s)	598
Bsc4I	CCNNNNNNNGG	9 cut(s)	176, 617, 1033, 1710, 2185, 2339, 2350, 2794, 2797
Bse118I	RCCGGY	1 cut(s)	598
Bse1I	ACTGG	7 cut(s)	811, 1323, 1456, 1783, 2143, 2229, 2977
Bse21I	CCTNAGG	1 cut(s)	81
Bse3DI	GCAATG	2 cut(s)	315, 1230
Bse8I	GATNNNNATC	1 cut(s)	3087
BseBI	CCWGG	5 cut(s)	736, 1187, 1366, 1436, 2110
BseJI	GATNNNNATC	1 cut(s)	3087
BseLI	CCNNNNNNNGG	9 cut(s)	176, 617, 1033, 1710, 2185, 2339, 2350, 2794, 2797
BseMI	GCAATG	2 cut(s)	315, 1230
BseMII	CTCAG	8 cut(s)	95, 282, 336, 1245, 1631, 2175, 2283, 2382
BseNI	ACTGG	7 cut(s)	811, 1323, 1456, 1783, 2143, 2229, 2977
BseRI	GAGGAG	3 cut(s)	129, 2660, 3179
BseSI	GKGCMC	4 cut(s)	562, 1735, 1749, 1961
BsgI	GTGCAG	1 cut(s)	2172
Bsh1236I	CGCG	3 cut(s)	115, 3068, 3153
Bsh1285I	CGRYCG	1 cut(s)	990
BshFI	GGCC	8 cut(s)	208, 985, 1026, 1885, 2343, 2538, 2787, 2883
BshNI	GGYRCC	3 cut(s)	1746, 2636, 2906
BshTI	ACCGGT	1 cut(s)	598
BsiEI	CGRYCG	1 cut(s)	990
BsiHKAI	GWGCWC	5 cut(s)	90, 297, 825, 1374, 3171
BsiSI	CCGG	6 cut(s)	205, 599, 759, 2318, 2839, 2867
BslFI	GGGAC	5 cut(s)	159, 1828, 2314, 2507, 2603
BslI	CCNNNNNNNGG	9 cut(s)	176, 617, 1033, 1710, 2185, 2339, 2350, 2794, 2797
BsmAI	GTCTC	4 cut(s)	325, 827, 1202, 1810
BsmFI	GGGAC	5 cut(s)	159, 1828, 2314, 2507, 2603
BsmI	GAATGC	3 cut(s)	1393, 2346, 2431
BsnI	GGCC	8 cut(s)	208, 985, 1026, 1885, 2343, 2538, 2787, 2883
Bsp1407I	TGTACA	1 cut(s)	2619
Bsp19I	CCATGG	1 cut(s)	2533
BspACI	CCGC	8 cut(s)	75, 608, 655, 1645, 2344, 2491, 2788, 2860
BspANI	GGCC	8 cut(s)	208, 985, 1026, 1885, 2343, 2538, 2787, 2883
BspCNI	CTCAG	8 cut(s)	94, 283, 335, 1246, 1632, 2174, 2282, 2383
BspFNI	CGCG	3 cut(s)	115, 3068, 3153
BspLI	GGNNCC	8 cut(s)	603, 733, 984, 1748, 2638, 2786, 2837, 2908
BspMAI	CTGCAG	2 cut(s)	153, 2382
BspPI	GGATC	4 cut(s)	895, 1149, 1163, 2686
BspT107I	GGYRCC	3 cut(s)	1746, 2636, 2906
BsrDI	GCAATG	2 cut(s)	315, 1230
BsrFI	RCCGGY	1 cut(s)	598
BsrGI	TGTACA	1 cut(s)	2619
BsrI	ACTGG	7 cut(s)	811, 1323, 1456, 1783, 2143, 2229, 2977
BssAI	RCCGGY	1 cut(s)	598
BssSI	CACGAG	2 cut(s)	2505, 2565
BssT1I	CCWWGG	8 cut(s)	554, 1027, 1735, 2068, 2337, 2403, 2533, 2815
Bst2BI	CACGAG	2 cut(s)	2505, 2565
Bst2UI	CCWGG	5 cut(s)	736, 1187, 1366, 1436, 2110
Bst6I	CTCTTC	1 cut(s)	1077
BstAUI	TGTACA	1 cut(s)	2619
BstC8I	GCNNGC	5 cut(s)	1399, 1580, 1584, 2245, 2652
BstDSI	CCRYGG	1 cut(s)	2533
BstFNI	CGCG	3 cut(s)	115, 3068, 3153
BstHHI	GCGC	1 cut(s)	3153
BstMAI	GTCTC	4 cut(s)	325, 827, 1202, 1810
BstMCI	CGRYCG	1 cut(s)	990
BstMWI	GCNNNNNNNGC	2 cut(s)	916, 1956
BstNI	CCWGG	5 cut(s)	736, 1187, 1366, 1436, 2110
BstNSI	RCATGY	2 cut(s)	1865, 2081
BstPAI	GACNNNNGTC	1 cut(s)	351
BstSCI	CCNGG	7 cut(s)	734, 758, 1185, 1364, 1434, 2108, 2316
BstSFI	CTRYAG	8 cut(s)	149, 342, 926, 1573, 1897, 2378, 2685, 3060
BstSLI	GKGCMC	4 cut(s)	562, 1735, 1749, 1961
BstUI	CGCG	3 cut(s)	115, 3068, 3153
BstV2I	GAAGAC	1 cut(s)	2163
BstX2I	RGATCY	3 cut(s)	1154, 2691, 3135
BstXI	CCANNNNNNTGG	1 cut(s)	2567
BstYI	RGATCY	3 cut(s)	1154, 2691, 3135
Bsu36I	CCTNAGG	1 cut(s)	81
BsuRI	GGCC	8 cut(s)	208, 985, 1026, 1885, 2343, 2538, 2787, 2883
BtgI	CCRYGG	1 cut(s)	2533
BtrI	CACGTC	1 cut(s)	2878
BtsI	GCAGTG	3 cut(s)	99, 1419, 3035
BtsIMutI	CAGTG	9 cut(s)	99, 278, 1161, 1210, 1419, 1776, 2742, 3035, 3165
Cac8I	GCNNGC	5 cut(s)	1399, 1580, 1584, 2245, 2652
CfoI	GCGC	1 cut(s)	3153
Cfr10I	RCCGGY	1 cut(s)	598
Cfr13I	GGNCC	5 cut(s)	551, 983, 2341, 2785, 2881
CseI	GACGC	4 cut(s)	49, 199, 1825, 3074
Csp6I	GTAC	8 cut(s)	111, 253, 1322, 1343, 1836, 1854, 2502, 2620
CspAI	ACCGGT	1 cut(s)	598
CviQI	GTAC	8 cut(s)	111, 253, 1322, 1343, 1836, 1854, 2502, 2620
DraI	TTTAAA	1 cut(s)	673
DraIII	CACNNNGTG	1 cut(s)	617
DrdI	GACNNNNNNGTC	1 cut(s)	2091
DseDI	GACNNNNNNGTC	1 cut(s)	2091
EaeI	YGGCCR	1 cut(s)	2536
Eam1104I	CTCTTC	1 cut(s)	1077
EarI	CTCTTC	1 cut(s)	1077
EciI	GGCGGA	1 cut(s)	597
Ecl136II	GAGCTC	5 cut(s)	88, 295, 823, 1372, 3169
Eco130I	CCWWGG	8 cut(s)	554, 1027, 1735, 2068, 2337, 2403, 2533, 2815
Eco24I	GRGCYC	6 cut(s)	90, 297, 825, 1374, 2656, 3171
Eco32I	GATATC	3 cut(s)	433, 1533, 1716
Eco47I	GGWCC	1 cut(s)	551
Eco53kI	GAGCTC	5 cut(s)	88, 295, 823, 1372, 3169
Eco57I	CTGAAG	2 cut(s)	254, 548
Eco81I	CCTNAGG	1 cut(s)	81
EcoICRI	GAGCTC	5 cut(s)	88, 295, 823, 1372, 3169
EcoO109I	RGGNCCY	1 cut(s)	551
EcoRII	CCWGG	5 cut(s)	734, 1185, 1364, 1434, 2108
EcoRV	GATATC	3 cut(s)	433, 1533, 1716
EcoT14I	CCWWGG	8 cut(s)	554, 1027, 1735, 2068, 2337, 2403, 2533, 2815
EcoT22I	ATGCAT	2 cut(s)	874, 2962
EcoT38I	GRGCYC	6 cut(s)	90, 297, 825, 1374, 2656, 3171
ErhI	CCWWGG	8 cut(s)	554, 1027, 1735, 2068, 2337, 2403, 2533, 2815
FalI	AAGNNNNNCTT	4 cut(s)	1794, 1826, 1998, 2030
FaqI	GGGAC	5 cut(s)	159, 1828, 2314, 2507, 2603
FauI	CCCGC	1 cut(s)	2484
FauNDI	CATATG	4 cut(s)	514, 949, 1953, 2308
FbaI	TGATCA	1 cut(s)	751
FblI	GTMKAC	1 cut(s)	3063
FriOI	GRGCYC	6 cut(s)	90, 297, 825, 1374, 2656, 3171
FspBI	CTAG	4 cut(s)	1349, 2069, 2148, 2816
GlaI	GCGC	1 cut(s)	3152
GsuI	CTGGAG	3 cut(s)	1071, 1377, 2960
HaeIII	GGCC	8 cut(s)	208, 985, 1026, 1885, 2343, 2538, 2787, 2883
HapII	CCGG	6 cut(s)	205, 599, 759, 2318, 2839, 2867
HgaI	GACGC	4 cut(s)	49, 199, 1825, 3074
HhaI	GCGC	1 cut(s)	3153
Hin6I	GCGC	1 cut(s)	3151
HinP1I	GCGC	1 cut(s)	3151
HincII	GTYRAC	3 cut(s)	994, 1788, 2734
HindII	GTYRAC	3 cut(s)	994, 1788, 2734
HindIII	AAGCTT	2 cut(s)	1195, 2045
HinfI	GANTC	9 cut(s)	902, 1098, 1228, 2159, 2485, 2563, 2870, 2887, 3098
HpaII	CCGG	6 cut(s)	205, 599, 759, 2318, 2839, 2867
Hpy99I	CGWCG	6 cut(s)	23, 65, 193, 2088, 2702, 2705
HpyCH4IV	ACGT	6 cut(s)	18, 45, 570, 1929, 2086, 2877
HpyF10VI	GCNNNNNNNGC	2 cut(s)	916, 1956
HpySE526I	ACGT	6 cut(s)	18, 45, 570, 1929, 2086, 2877
HspAI	GCGC	1 cut(s)	3151
Ksp22I	TGATCA	1 cut(s)	751
LmnI	GCTCC	9 cut(s)	85, 1052, 1369, 1396, 2101, 2647, 2717, 2979, 3166
LweI	GCATC	9 cut(s)	268, 296, 712, 854, 925, 1121, 1291, 1656, 1883
MaeI	CTAG	4 cut(s)	1349, 2069, 2148, 2816
MaeII	ACGT	6 cut(s)	18, 45, 570, 1929, 2086, 2877
MaeIII	GTNAC	6 cut(s)	22, 46, 802, 1039, 1327, 2442
MfeI	CAATTG	4 cut(s)	465, 840, 1995, 2028
MflI	RGATCY	3 cut(s)	1154, 2691, 3135
MlsI	TGGCCA	1 cut(s)	2538
MluNI	TGGCCA	1 cut(s)	2538
MlyI	GAGTC	4 cut(s)	2153, 2479, 2557, 2864
MmeI	TCCRAC	5 cut(s)	1224, 1573, 2166, 2915, 2989
Mox20I	TGGCCA	1 cut(s)	2538
Mph1103I	ATGCAT	2 cut(s)	874, 2962
MroXI	GAANNNNTTC	2 cut(s)	2429, 3074
MscI	TGGCCA	1 cut(s)	2538
MseI	TTAA	9 cut(s)	399, 593, 672, 1151, 1410, 1754, 2457, 2594, 2615
Msp20I	TGGCCA	1 cut(s)	2538
MspA1I	CMGCKG	2 cut(s)	1582, 1589
MspI	CCGG	6 cut(s)	205, 599, 759, 2318, 2839, 2867
MspR9I	CCNGG	7 cut(s)	736, 760, 1187, 1366, 1436, 2110, 2318
MunI	CAATTG	4 cut(s)	465, 840, 1995, 2028
Mva1269I	GAATGC	3 cut(s)	1393, 2346, 2431
MvaI	CCWGG	5 cut(s)	736, 1187, 1366, 1436, 2110
MvnI	CGCG	3 cut(s)	115, 3068, 3153
MwoI	GCNNNNNNNGC	2 cut(s)	916, 1956
NciI	CCSGG	2 cut(s)	760, 2318
NcoI	CCATGG	1 cut(s)	2533
NdeI	CATATG	4 cut(s)	514, 949, 1953, 2308
NlaIV	GGNNCC	8 cut(s)	603, 733, 984, 1748, 2638, 2786, 2837, 2908
NmuCI	GTSAC	4 cut(s)	46, 802, 1039, 1327
NsiI	ATGCAT	2 cut(s)	874, 2962
NspI	RCATGY	2 cut(s)	1865, 2081
PcsI	WCGNNNNNNNCGW	2 cut(s)	334, 2426
PctI	GAATGC	3 cut(s)	1393, 2346, 2431
PdmI	GAANNNNTTC	2 cut(s)	2429, 3074
PfeI	GAWTC	5 cut(s)	902, 1098, 1228, 2887, 3098
PflMI	CCANNNNNTGG	2 cut(s)	176, 617
PfoI	TCCNGGA	2 cut(s)	2108, 2316
PinAI	ACCGGT	1 cut(s)	598
Ple19I	CGATCG	1 cut(s)	990
PleI	GAGTC	4 cut(s)	2153, 2479, 2557, 2864
PpsI	GAGTC	4 cut(s)	2153, 2479, 2557, 2864
PpuMI	RGGWCCY	1 cut(s)	551
PshAI	GACNNNNGTC	1 cut(s)	351
PshBI	ATTAAT	2 cut(s)	1410, 2457
PsiI	TTATAA	2 cut(s)	782, 2664
Psp124BI	GAGCTC	5 cut(s)	90, 297, 825, 1374, 3171
Psp1406I	AACGTT	1 cut(s)	570
Psp5II	RGGWCCY	1 cut(s)	551
Psp6I	CCWGG	5 cut(s)	734, 1185, 1364, 1434, 2108
PspGI	CCWGG	5 cut(s)	734, 1185, 1364, 1434, 2108
PspN4I	GGNNCC	8 cut(s)	603, 733, 984, 1748, 2638, 2786, 2837, 2908
PspPI	GGNCC	5 cut(s)	551, 983, 2341, 2785, 2881
PspPPI	RGGWCCY	1 cut(s)	551
PstI	CTGCAG	2 cut(s)	153, 2382
PsuI	RGATCY	3 cut(s)	1154, 2691, 3135
PvuI	CGATCG	1 cut(s)	990
PvuII	CAGCTG	2 cut(s)	1582, 1589
RsaI	GTAC	8 cut(s)	112, 254, 1323, 1344, 1837, 1855, 2503, 2621
RsaNI	GTAC	8 cut(s)	111, 253, 1322, 1343, 1836, 1854, 2502, 2620
SacI	GAGCTC	5 cut(s)	90, 297, 825, 1374, 3171
SaqAI	TTAA	9 cut(s)	399, 593, 672, 1151, 1410, 1754, 2457, 2594, 2615
Sau96I	GGNCC	5 cut(s)	551, 983, 2341, 2785, 2881
ScaI	AGTACT	1 cut(s)	1855
SchI	GAGTC	4 cut(s)	2153, 2479, 2557, 2864
ScrFI	CCNGG	7 cut(s)	736, 760, 1187, 1366, 1436, 2110, 2318
SfaNI	GCATC	9 cut(s)	268, 296, 712, 854, 925, 1121, 1291, 1656, 1883
SfcI	CTRYAG	8 cut(s)	149, 342, 926, 1573, 1897, 2378, 2685, 3060
SinI	GGWCC	1 cut(s)	551
SmlI	CTYRAG	9 cut(s)	386, 410, 1013, 1232, 1275, 1373, 2792, 2946, 2986
SmoI	CTYRAG	9 cut(s)	386, 410, 1013, 1232, 1275, 1373, 2792, 2946, 2986
SpeI	ACTAGT	1 cut(s)	1348
SsiI	CCGC	8 cut(s)	75, 608, 655, 1645, 2344, 2491, 2788, 2860
SspI	AATATT	1 cut(s)	646
SspMI	CTAG	4 cut(s)	1349, 2069, 2148, 2816
SstI	GAGCTC	5 cut(s)	90, 297, 825, 1374, 3171
StyD4I	CCNGG	7 cut(s)	734, 758, 1185, 1364, 1434, 2108, 2316
StyI	CCWWGG	8 cut(s)	554, 1027, 1735, 2068, 2337, 2403, 2533, 2815
TaiI	ACGT	6 cut(s)	21, 48, 573, 1932, 2089, 2880
TaqI	TCGA	4 cut(s)	546, 1008, 1406, 2697
TatI	WGTACW	2 cut(s)	1853, 2619
TauI	GCSGC	3 cut(s)	1647, 2346, 2790
TfiI	GAWTC	5 cut(s)	902, 1098, 1228, 2887, 3098
Tru1I	TTAA	9 cut(s)	399, 593, 672, 1151, 1410, 1754, 2457, 2594, 2615
Tru9I	TTAA	9 cut(s)	399, 593, 672, 1151, 1410, 1754, 2457, 2594, 2615
TscAI	CASTG	9 cut(s)	106, 278, 1168, 1210, 1426, 1783, 2749, 3035, 3165
TseFI	GTSAC	4 cut(s)	46, 802, 1039, 1327
Tsp45I	GTSAC	4 cut(s)	46, 802, 1039, 1327
TspDTI	ATGAA	9 cut(s)	846, 936, 1119, 1241, 2039, 2164, 2347, 2566, 2612
TspGWI	ACGGA	2 cut(s)	1805, 1823
TspRI	CASTG	9 cut(s)	106, 278, 1168, 1210, 1426, 1783, 2749, 3035, 3165
Van91I	CCANNNNNTGG	2 cut(s)	176, 617
VpaK11BI	GGWCC	1 cut(s)	551
VspI	ATTAAT	2 cut(s)	1410, 2457
XapI	RAATTY	4 cut(s)	123, 257, 2725, 3073
XceI	RCATGY	2 cut(s)	1865, 2081
XmaJI	CCTAGG	2 cut(s)	2068, 2815
XmiI	GTMKAC	1 cut(s)	3063
XmnI	GAANNNNTTC	2 cut(s)	2429, 3074
XspI	CTAG	4 cut(s)	1349, 2069, 2148, 2816
ZrmI	AGTACT	1 cut(s)	1855
Zsp2I	ATGCAT	2 cut(s)	874, 2962

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.