pycom10g22150

UDP-N-acetylglucosamine transferase subunit ALG14

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0006662
Physical Location & Seq
Forward (+)
24607662 .. 24608393
732 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 528 bp
ATGGGATGGGCCGTTGCAGTCACTCTTCTGATCGCCGCCGCAACGCTGATCGTTACTCGCCTGCTCTACGTATTGCACCGAACCGGCAAACCCCACAAGCCCAGCAGCTCAAAAGGCTCAGCTCCCCAACCCCTCAGCACCCTAATCGTTTTGGGCTCTGGAGGCCACACGGCGGAGATGATCAACCTCTTGTCAGTCCTTCAAAGAGACAGATTTGCCCCTAGGTTCTACATCGCCGCCGCCACTGATAACATGAGCCTCCAGAAAGCTCGCCTGCTGGAAGAGAACGCCGATGAGAGTTCGAGCTCGCAGTTCATGCAGATCGACCGTAGTAGGGAAGTGGGTCAGTCTTATTTCACCTCTGTTTTGACCACTTTGATTGCTTTGGCTCATGGGCTCTGGCTAATCGATCAGAATCCGACCCCAAGTGATTCTGTGCAATGGGCCTGGGACTTGTGTTCCACTTTGTGTAATTGCATTCATATTCAAGGTTGTGGGGATTCGATGGTCGTCTGTGTTTTATGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00510 ko00513 ko01100 map00510 map00513 map01100
Pfam Domains
Protein Families
Alg14

Protein Analysis

176

Amino Acids

19.03

Weight (kDa)

6.49

Isoelectric Point (pI)

39.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Alg14 PF08660 48 - 173 3.5e-28 Oligosaccharide biosynthesis protein Alg14 like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 5 cut(s) 36, 39, 173, 237, 240
AdeI CACNNNGTG 1 cut(s) 468
AfiI CCNNNNNNNGG 2 cut(s) 172, 334
AgsI TTSAA 2 cut(s) 203, 488
AjnI CCWGG 1 cut(s) 446
AluBI AGCT 4 cut(s) 108, 122, 269, 306
AluI AGCT 4 cut(s) 108, 122, 269, 306
Alw21I GWGCWC 1 cut(s) 308
Alw26I GTCTC 1 cut(s) 201
AoxI GGCC 3 cut(s) 9, 163, 444
ApeKI GCWGC 1 cut(s) 105
AspA2I CCTAGG 1 cut(s) 221
AspS9I GGNCC 2 cut(s) 9, 444
AsuHPI GGTGA 1 cut(s) 349
AvrII CCTAGG 1 cut(s) 221
BanII GRGCYC 3 cut(s) 158, 308, 399
Bbv12I GWGCWC 1 cut(s) 308
BbvCI CCTCAGC 1 cut(s) 134
BbvI GCAGC 1 cut(s) 117
BccI CCATC 1 cut(s) 499
BceAI ACGGC 1 cut(s) 186
BcgI CGANNNNNNTGC 2 cut(s) 127, 161
BciT130I CCWGG 1 cut(s) 448
BclI TGATCA 1 cut(s) 180
BcoDI GTCTC 1 cut(s) 201
BfaI CTAG 1 cut(s) 222
BisI GCNGC 5 cut(s) 36, 39, 106, 237, 240
BlnI CCTAGG 1 cut(s) 221
BlpI GCTNAGC 1 cut(s) 118
BlsI GCNGC 5 cut(s) 37, 40, 107, 238, 241
Bme1390I CCNGG 1 cut(s) 448
BmgT120I GGNCC 2 cut(s) 9, 444
BmrFI CCNGG 1 cut(s) 448
BpmI CTGGAG 2 cut(s) 180, 245
Bpu10I CCTNAGC 1 cut(s) 134
Bpu1102I GCTNAGC 1 cut(s) 118
Bsa29I ATCGAT 1 cut(s) 408
BsaAI YACGTR 1 cut(s) 70
BsaBI GATNNNNATC 1 cut(s) 414
BsaJI CCNNGG 2 cut(s) 221, 447
Bsc4I CCNNNNNNNGG 2 cut(s) 172, 334
Bse118I RCCGGY 1 cut(s) 83
Bse3DI GCAATG 1 cut(s) 446
Bse8I GATNNNNATC 1 cut(s) 414
BseBI CCWGG 1 cut(s) 448
BseCI ATCGAT 1 cut(s) 408
BseDI CCNNGG 2 cut(s) 221, 447
BseGI GGATG 1 cut(s) 11
BseJI GATNNNNATC 1 cut(s) 414
BseLI CCNNNNNNNGG 2 cut(s) 172, 334
BseMI GCAATG 1 cut(s) 446
BseMII CTCAG 2 cut(s) 132, 148
BseXI GCAGC 1 cut(s) 117
BseYI CCCAGC 1 cut(s) 101
Bsh1285I CGRYCG 1 cut(s) 328
BshFI GGCC 3 cut(s) 11, 165, 446
BshVI ATCGAT 1 cut(s) 408
BsiEI CGRYCG 1 cut(s) 328
BsiHKAI GWGCWC 1 cut(s) 308
BsiSI CCGG 1 cut(s) 84
BslFI GGGAC 1 cut(s) 464
BslI CCNNNNNNNGG 2 cut(s) 172, 334
BsmAI GTCTC 1 cut(s) 201
BsmFI GGGAC 1 cut(s) 464
BsmI GAATGC 1 cut(s) 477
BsnI GGCC 3 cut(s) 11, 165, 446
Bsp1286I GDGCHC 3 cut(s) 158, 308, 399
Bsp143I GATC 5 cut(s) 30, 48, 180, 321, 409
Bsp1720I GCTNAGC 1 cut(s) 118
BspACI CCGC 5 cut(s) 36, 39, 173, 237, 240
BspANI GGCC 3 cut(s) 11, 165, 446
BspCNI CTCAG 2 cut(s) 131, 147
BspDI ATCGAT 1 cut(s) 408
BsrDI GCAATG 1 cut(s) 446
BsrFI RCCGGY 1 cut(s) 83
BssAI RCCGGY 1 cut(s) 83
BssECI CCNNGG 2 cut(s) 221, 447
BssMI GATC 5 cut(s) 30, 48, 180, 321, 409
BssT1I CCWWGG 1 cut(s) 221
Bst2UI CCWGG 1 cut(s) 448
Bst4CI ACNGT 1 cut(s) 329
Bst6I CTCTTC 2 cut(s) 30, 276
BstAPI GCANNNNNTGC 1 cut(s) 316
BstBAI YACGTR 1 cut(s) 70
BstC8I GCNNGC 4 cut(s) 62, 271, 275, 308
BstDEI CTNAG 2 cut(s) 118, 134
BstF5I GGATG 1 cut(s) 11
BstKTI GATC 5 cut(s) 33, 51, 183, 324, 412
BstMAI GTCTC 1 cut(s) 201
BstMBI GATC 5 cut(s) 30, 48, 180, 321, 409
BstMCI CGRYCG 1 cut(s) 328
BstMWI GCNNNNNNNGC 3 cut(s) 114, 162, 316
BstNI CCWGG 1 cut(s) 448
BstSCI CCNGG 1 cut(s) 446
BstSNI TACGTA 1 cut(s) 70
BstV1I GCAGC 1 cut(s) 117
Bsu15I ATCGAT 1 cut(s) 408
BsuRI GGCC 3 cut(s) 11, 165, 446
BsuTUI ATCGAT 1 cut(s) 408
BtgZI GCGATG 1 cut(s) 217
BtsCI GGATG 1 cut(s) 11
BtsIMutI CAGTG 1 cut(s) 243
Cac8I GCNNGC 4 cut(s) 62, 271, 275, 308
Cfr10I RCCGGY 1 cut(s) 83
Cfr13I GGNCC 2 cut(s) 9, 444
ClaI ATCGAT 1 cut(s) 408
CspCI CAANNNNNGTGG 2 cut(s) 361, 396
CviAII CATG 3 cut(s) 253, 316, 392
DdeI CTNAG 2 cut(s) 118, 134
DpnI GATC 5 cut(s) 32, 50, 182, 323, 411
DpnII GATC 5 cut(s) 30, 48, 180, 321, 409
DraIII CACNNNGTG 1 cut(s) 468
Eam1104I CTCTTC 2 cut(s) 30, 276
EarI CTCTTC 2 cut(s) 30, 276
EciI GGCGGA 1 cut(s) 188
Ecl136II GAGCTC 1 cut(s) 306
Eco105I TACGTA 1 cut(s) 70
Eco130I CCWWGG 1 cut(s) 221
Eco24I GRGCYC 3 cut(s) 158, 308, 399
Eco53kI GAGCTC 1 cut(s) 306
EcoICRI GAGCTC 1 cut(s) 306
EcoRII CCWGG 1 cut(s) 446
EcoT14I CCWWGG 1 cut(s) 221
EcoT38I GRGCYC 3 cut(s) 158, 308, 399
ErhI CCWWGG 1 cut(s) 221
FaeI CATG 3 cut(s) 256, 319, 395
FaiI YATR 5 cut(s) 254, 317, 393, 483, 523
FaqI GGGAC 1 cut(s) 464
FatI CATG 3 cut(s) 252, 315, 391
FbaI TGATCA 1 cut(s) 180
Fnu4HI GCNGC 5 cut(s) 36, 39, 106, 237, 240
FokI GGATG 1 cut(s) 18
FriOI GRGCYC 3 cut(s) 158, 308, 399
Fsp4HI GCNGC 5 cut(s) 36, 39, 106, 237, 240
FspBI CTAG 1 cut(s) 222
GluI GCNGC 5 cut(s) 36, 39, 106, 237, 240
GsaI CCCAGC 1 cut(s) 105
GsuI CTGGAG 2 cut(s) 180, 245
HaeIII GGCC 3 cut(s) 11, 165, 446
HapII CCGG 1 cut(s) 84
Hin1II CATG 3 cut(s) 256, 319, 395
HinfI GANTC 3 cut(s) 415, 431, 500
HpaII CCGG 1 cut(s) 84
HphI GGTGA 1 cut(s) 349
Hpy188I TCNGA 3 cut(s) 30, 414, 420
Hpy188III TCNNGA 2 cut(s) 159, 262
HpyAV CCTTC 1 cut(s) 209
HpyCH4III ACNGT 1 cut(s) 329
HpyCH4IV ACGT 1 cut(s) 69
HpyCH4V TGCA 5 cut(s) 17, 76, 319, 439, 477
HpyF10VI GCNNNNNNNGC 3 cut(s) 114, 162, 316
HpyF3I CTNAG 2 cut(s) 118, 134
HpySE526I ACGT 1 cut(s) 69
Hsp92II CATG 3 cut(s) 256, 319, 395
Ksp22I TGATCA 1 cut(s) 180
Kzo9I GATC 5 cut(s) 30, 48, 180, 321, 409
LmnI GCTCC 1 cut(s) 127
Lsp1109I GCAGC 1 cut(s) 117
MaeI CTAG 1 cut(s) 222
MaeII ACGT 1 cut(s) 69
MaeIII GTNAC 2 cut(s) 19, 52
MalI GATC 5 cut(s) 32, 50, 182, 323, 411
MboI GATC 5 cut(s) 30, 48, 180, 321, 409
MboII GAAGA 2 cut(s) 17, 293
MhlI GDGCHC 3 cut(s) 158, 308, 399
MluCI AATT 1 cut(s) 472
MmeI TCCRAC 1 cut(s) 443
MnlI CCTC 5 cut(s) 143, 155, 197, 269, 370
MspI CCGG 1 cut(s) 84
MspR9I CCNGG 1 cut(s) 448
Mva1269I GAATGC 1 cut(s) 477
MvaI CCWGG 1 cut(s) 448
MwoI GCNNNNNNNGC 3 cut(s) 114, 162, 316
NdeII GATC 5 cut(s) 30, 48, 180, 321, 409
NlaIII CATG 3 cut(s) 256, 319, 395
NmuCI GTSAC 1 cut(s) 19
PctI GAATGC 1 cut(s) 477
PfeI GAWTC 3 cut(s) 415, 431, 500
PkrI GCNGC 5 cut(s) 37, 40, 107, 238, 241
Ppu21I YACGTR 1 cut(s) 70
Psp124BI GAGCTC 1 cut(s) 308
Psp6I CCWGG 1 cut(s) 446
PspFI CCCAGC 1 cut(s) 101
PspGI CCWGG 1 cut(s) 446
PspPI GGNCC 2 cut(s) 9, 444
SacI GAGCTC 1 cut(s) 308
SatI GCNGC 5 cut(s) 36, 39, 106, 237, 240
Sau3AI GATC 5 cut(s) 30, 48, 180, 321, 409
Sau96I GGNCC 2 cut(s) 9, 444
ScrFI CCNGG 1 cut(s) 448
SduI GDGCHC 3 cut(s) 158, 308, 399
SetI ASST 9 cut(s) 72, 110, 124, 189, 227, 271, 308, 362, 493
SnaBI TACGTA 1 cut(s) 70
Sse9I AATT 1 cut(s) 472
SsiI CCGC 5 cut(s) 36, 39, 173, 237, 240
SspMI CTAG 1 cut(s) 222
SstI GAGCTC 1 cut(s) 308
StyD4I CCNGG 1 cut(s) 446
StyI CCWWGG 1 cut(s) 221
TaaI ACNGT 1 cut(s) 329
TaiI ACGT 1 cut(s) 72
TaqI TCGA 4 cut(s) 302, 324, 408, 503
TasI AATT 1 cut(s) 472
TauI GCSGC 4 cut(s) 38, 41, 239, 242
TfiI GAWTC 3 cut(s) 415, 431, 500
TscAI CASTG 1 cut(s) 250
TseFI GTSAC 1 cut(s) 19
TseI GCWGC 1 cut(s) 105
Tsp45I GTSAC 1 cut(s) 19
TspDTI ATGAA 2 cut(s) 304, 470
TspRI CASTG 1 cut(s) 250
XmaJI CCTAGG 1 cut(s) 221
XspI CTAG 1 cut(s) 222
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.