pycom10g22230

Potassium channel

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0009345
Physical Location & Seq
Reverse (-)
24662747 .. 24666240
3494 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1293 bp
ATGGAGAAAGAGCCGTTGCTGCCATACGTAGGCAGTCCGAGAAGAAAGCTGCCATCTTTATCGAATCTCTGGCCACTTCCAGAAGACGATGAATTCACTATCTCTTTAACGCCTTCGGAGTTCAAAGACCGTATAATCTTTGGTCCTGCAACTGCTTCGCCTCGAGATTCTTCACCTCTCGTCGATGCATTGACTCTGCCTATCCATTCTCCAAGAACTTCAAGACCCCCTTCGTCTTCTTCTCTATTGGACTCCATAGCCACAACCCAACAGGACCCACAATCCCAACAGCCACCAACTCCGCAATCATGGCTCATTGACCCCAATTACTCGTGGACCAAAACCAACCTCCACCGCTCGAAAACAGCTCCTGCCATGGCCGTGATCAACGAGGTGGCCAACCGGCGCTCTGTTCCCAGACCCCAGTTCGGTTCGCAGTCGATTGTAAGACAAGGTGTTGTACTTCTTGTTATATATCTTGCATTGGGTGTGATTATATATTGGTTTAACCGTGATCATTTTGCGGCTCTGGAGACGCACCCTGTGGTTGATGCATTGTACTTCTGTATTGTGACAATGTGCACAATTGGGTATGGAGATATTACGCCTACGACTACTGCAACCAAGTTGTTTTCGATAGCGTTTGTGTTGGTGGGGTTCGGATTTGTAGACATTTTGCTGAGTGGGATGGTTAGTTATGTGCTTGATTTGCAAGAGAATTATTTTTTGAGGAGCCTTAAGGGTGTGGGTGAGAAAAAGGAGTCTTACATATTTGATGTTAAGAAGGGGAGGATGAGGATTAGGATGAAGGTGGGATTGGCATTGGGAGTTGTGGTTCTTTGCATCGGGATCGGTGTGGGTGTTATGCATTTTGTGGAAAAGCTTGGATGGTTGGATTCATTTTATCTTTCGGTTATGTCAGTTACCACAGTTGGGTATGGCGATCGGGCTTTTCAGTCTTTACCAGGCCGCATCTTTGCTTCAGTTTGGTTGCTTGTGTCGACGCTTGCAGTTGCTCGAGCATTTTTGTACTTGGCTGAGGCTAGGGTGGATAAACGGCATAGGAAGATGGCGAAGTGGGTTCTTGGTCAGGATATGACGGTTTCCGAGTTTCTTGCTGCTGACATTGACAATAACGGCTTTGTGAGTAAGTCGGAATATGTCGTATACAAACTCAAGGAGATGGGAAAGGTATCAGAGAAAGATGTAATGCAGATCTGCACCCAATTTGATAGGCTAGACACAGGAAACTGCGGAAAGATAAGCCTTGCTGATCTCTTGAGTCGACATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000325 GO:0003674 GO:0003824 GO:0004788 GO:0005215 GO:0005216 GO:0005261 GO:0005267 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0005829 GO:0005886 GO:0005887 GO:0006725 GO:0006732 GO:0006766 GO:0006767 GO:0006772 GO:0006790 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006811 GO:0006812 GO:0006813 GO:0006996 GO:0008150 GO:0008152 GO:0008324 GO:0009058 GO:0009108 GO:0009229 GO:0009314 GO:0009416 GO:0009507 GO:0009532 GO:0009533 GO:0009534 GO:0009536 GO:0009570 GO:0009579 GO:0009628 GO:0009657 GO:0009668 GO:0009705 GO:0009987 GO:0010027 GO:0010196 GO:0015075 GO:0015077 GO:0015079 GO:0015267 GO:0015318 GO:0015672 GO:0016020 GO:0016021 GO:0016043 GO:0016740 GO:0016772 GO:0016778 GO:0017144 GO:0018130 GO:0019438 GO:0019637 GO:0022607 GO:0022803 GO:0022838 GO:0022840 GO:0022841 GO:0022842 GO:0022857 GO:0022890 GO:0030001 GO:0030322 GO:0031090 GO:0031224 GO:0031226 GO:0031976 GO:0031984 GO:0034220 GO:0034641 GO:0042357 GO:0042391 GO:0042723 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043933 GO:0044085 GO:0044237 GO:0044249 GO:0044271 GO:0044272 GO:0044281 GO:0044422 GO:0044424 GO:0044425 GO:0044434 GO:0044435 GO:0044437 GO:0044444 GO:0044446 GO:0044459 GO:0044464 GO:0046483 GO:0046873 GO:0050896 GO:0051179 GO:0051186 GO:0051188 GO:0051234 GO:0051259 GO:0051260 GO:0055085 GO:0061024 GO:0065003 GO:0065007 GO:0065008 GO:0071704 GO:0071804 GO:0071805 GO:0071840 GO:0071944 GO:0072527 GO:0072528 GO:0090407 GO:0098588 GO:0098655 GO:0098660 GO:0098662 GO:0098805 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576 GO:1990066
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ion_trans_2

Protein Analysis

431

Amino Acids

47.92

Weight (kDa)

8.92

Isoelectric Point (pI)

42.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ion_trans PF00520 153 - 234 2.8e-06 Ion transport protein
Ion_trans_2 PF07885 155 - 234 5.2e-16 Ion channel
Ion_trans_2 PF07885 278 - 347 1.6e-11 Ion channel
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 357
AccI GTMKAC 4 cut(s) 669, 1001, 1167, 1285
AciI CCGC 5 cut(s) 302, 355, 524, 970, 1254
AclWI GGATC 1 cut(s) 857
AcoI YGGCCR 3 cut(s) 71, 378, 396
AcsI RAATTY 1 cut(s) 92
AcuI CTGAAG 1 cut(s) 966
AdeI CACNNNGTG 1 cut(s) 544
AfaI GTAC 3 cut(s) 462, 560, 1031
AfiI CCNNNNNNNGG 3 cut(s) 29, 428, 933
AflII CTTAAG 1 cut(s) 737
AgsI TTSAA 2 cut(s) 124, 222
AjnI CCWGG 1 cut(s) 964
AjuI GAANNNNNNNTTGG 2 cut(s) 800, 832
AloI GAACNNNNNNTCC 2 cut(s) 819, 851
AluBI AGCT 3 cut(s) 49, 368, 883
AluI AGCT 3 cut(s) 49, 368, 883
Alw21I GWGCWC 1 cut(s) 584
Alw26I GTCTC 1 cut(s) 527
Alw44I GTGCAC 1 cut(s) 580
AlwI GGATC 1 cut(s) 857
AlwNI CAGNNNCTG 1 cut(s) 371
Ama87I CYCGRG 2 cut(s) 162, 1017
AoxI GGCC 4 cut(s) 71, 378, 396, 967
ApaLI GTGCAC 1 cut(s) 580
ApeKI GCWGC 3 cut(s) 19, 49, 1118
ApoI RAATTY 1 cut(s) 92
AspLEI GCGC 1 cut(s) 408
AspS9I GGNCC 3 cut(s) 143, 274, 336
AsuHPI GGTGA 2 cut(s) 165, 761
AvaI CYCGRG 2 cut(s) 162, 1017
AvaII GGWCC 3 cut(s) 143, 274, 336
BaeGI GKGCMC 1 cut(s) 584
BalI TGGCCA 2 cut(s) 73, 398
BauI CACGAG 1 cut(s) 331
BbsI GAAGAC 2 cut(s) 90, 228
Bbv12I GWGCWC 1 cut(s) 584
BbvCI CCTCAGC 1 cut(s) 1038
BbvI GCAGC 3 cut(s) 6, 36, 1105
BccI CCATC 5 cut(s) 61, 682, 882, 1063, 1177
BceAI ACGGC 3 cut(s) 365, 1073, 1153
BcgI CGANNNNNNTGC 6 cut(s) 138, 172, 832, 866, 1097, 1131
BciT130I CCWGG 1 cut(s) 966
BclI TGATCA 2 cut(s) 384, 514
BcoDI GTCTC 1 cut(s) 527
BfaI CTAG 2 cut(s) 1044, 1238
BfoI RGCGCY 1 cut(s) 409
BfrI CTTAAG 1 cut(s) 737
BglII AGATCT 1 cut(s) 1215
BisI GCNGC 5 cut(s) 20, 50, 525, 970, 1119
BlsI GCNGC 5 cut(s) 21, 51, 526, 971, 1120
Bme1390I CCNGG 1 cut(s) 966
Bme18I GGWCC 3 cut(s) 143, 274, 336
BmeT110I CYCGRG 2 cut(s) 162, 1017
BmgT120I GGNCC 3 cut(s) 143, 274, 336
BmiI GGNNCC 2 cut(s) 276, 734
BmrFI CCNGG 1 cut(s) 966
BmrI ACTGGG 1 cut(s) 418
BmsI GCATC 4 cut(s) 175, 541, 852, 981
BmuI ACTGGG 1 cut(s) 418
BpiI GAAGAC 2 cut(s) 90, 228
BpmI CTGGAG 1 cut(s) 551
Bpu10I CCTNAGC 1 cut(s) 1038
BpuEI CTTGAG 1 cut(s) 1160
BsaAI YACGTR 1 cut(s) 28
BsaJI CCNNGG 1 cut(s) 375
BsaXI ACNNNNNCTCC 2 cut(s) 819, 849
Bsc4I CCNNNNNNNGG 3 cut(s) 29, 428, 933
Bse118I RCCGGY 1 cut(s) 402
Bse1I ACTGG 1 cut(s) 424
BseBI CCWGG 1 cut(s) 966
BseDI CCNNGG 1 cut(s) 375
BseGI GGATG 4 cut(s) 693, 798, 810, 893
BseLI CCNNNNNNNGG 3 cut(s) 29, 428, 933
BseMII CTCAG 2 cut(s) 671, 1029
BseNI ACTGG 1 cut(s) 424
BseRI GAGGAG 1 cut(s) 745
BseSI GKGCMC 1 cut(s) 584
BseXI GCAGC 3 cut(s) 6, 36, 1105
BsgI GTGCAG 1 cut(s) 1204
Bsh1285I CGRYCG 1 cut(s) 946
BshFI GGCC 4 cut(s) 73, 380, 398, 969
BsiEI CGRYCG 1 cut(s) 946
BsiHKAI GWGCWC 1 cut(s) 584
BsiHKCI CYCGRG 2 cut(s) 162, 1017
BsiSI CCGG 1 cut(s) 403
BslI CCNNNNNNNGG 3 cut(s) 29, 428, 933
BsmAI GTCTC 1 cut(s) 527
BsmBI CGTCTC 1 cut(s) 527
BsnI GGCC 4 cut(s) 73, 380, 398, 969
BsoBI CYCGRG 2 cut(s) 162, 1017
Bsp1286I GDGCHC 1 cut(s) 584
Bsp143I GATC 6 cut(s) 384, 514, 849, 943, 1215, 1273
Bsp19I CCATGG 1 cut(s) 375
BspACI CCGC 5 cut(s) 302, 355, 524, 970, 1254
BspANI GGCC 4 cut(s) 73, 380, 398, 969
BspCNI CTCAG 2 cut(s) 672, 1030
BspLI GGNNCC 2 cut(s) 276, 734
BspPI GGATC 1 cut(s) 857
BspTI CTTAAG 1 cut(s) 737
BsrBI CCGCTC 1 cut(s) 357
BsrFI RCCGGY 1 cut(s) 402
BsrI ACTGG 1 cut(s) 424
BssAI RCCGGY 1 cut(s) 402
BssECI CCNNGG 1 cut(s) 375
BssMI GATC 6 cut(s) 384, 514, 849, 943, 1215, 1273
BssNAI GTATAC 1 cut(s) 1168
BssSI CACGAG 1 cut(s) 331
BssT1I CCWWGG 1 cut(s) 375
Bst1107I GTATAC 1 cut(s) 1168
Bst2BI CACGAG 1 cut(s) 331
Bst2UI CCWGG 1 cut(s) 966
Bst4CI ACNGT 4 cut(s) 131, 512, 931, 1102
BstAFI CTTAAG 1 cut(s) 737
BstBAI YACGTR 1 cut(s) 28
BstC8I GCNNGC 1 cut(s) 1008
BstDEI CTNAG 2 cut(s) 680, 1038
BstDSI CCRYGG 1 cut(s) 375
BstF5I GGATG 4 cut(s) 693, 798, 810, 893
BstH2I RGCGCY 1 cut(s) 409
BstHHI GCGC 1 cut(s) 408
BstKTI GATC 6 cut(s) 387, 517, 852, 946, 1218, 1276
BstMAI GTCTC 1 cut(s) 527
BstMBI GATC 6 cut(s) 384, 514, 849, 943, 1215, 1273
BstMCI CGRYCG 1 cut(s) 946
BstMWI GCNNNNNNNGC 3 cut(s) 19, 310, 709
BstNI CCWGG 1 cut(s) 966
BstSCI CCNGG 1 cut(s) 964
BstSLI GKGCMC 1 cut(s) 584
BstSNI TACGTA 1 cut(s) 28
BstV1I GCAGC 3 cut(s) 6, 36, 1105
BstV2I GAAGAC 2 cut(s) 90, 228
BstX2I RGATCY 1 cut(s) 1215
BstYI RGATCY 1 cut(s) 1215
BstZ17I GTATAC 1 cut(s) 1168
BsuRI GGCC 4 cut(s) 73, 380, 398, 969
BtgI CCRYGG 1 cut(s) 375
BtsCI GGATG 4 cut(s) 693, 798, 810, 893
Cac8I GCNNGC 1 cut(s) 1008
CaiI CAGNNNCTG 1 cut(s) 371
CfoI GCGC 1 cut(s) 408
Cfr10I RCCGGY 1 cut(s) 402
Cfr13I GGNCC 3 cut(s) 143, 274, 336
CseI GACGC 2 cut(s) 544, 1012
Csp6I GTAC 3 cut(s) 461, 559, 1030
CviAII CATG 2 cut(s) 309, 376
CviQI GTAC 3 cut(s) 461, 559, 1030
DdeI CTNAG 2 cut(s) 680, 1038
DpnI GATC 6 cut(s) 386, 516, 851, 945, 1217, 1275
DpnII GATC 6 cut(s) 384, 514, 849, 943, 1215, 1273
DraIII CACNNNGTG 1 cut(s) 544
EaeI YGGCCR 3 cut(s) 71, 378, 396
Eco105I TACGTA 1 cut(s) 28
Eco130I CCWWGG 1 cut(s) 375
Eco47I GGWCC 3 cut(s) 143, 274, 336
Eco57I CTGAAG 1 cut(s) 966
Eco88I CYCGRG 2 cut(s) 162, 1017
EcoO109I RGGNCCY 1 cut(s) 274
EcoRI GAATTC 1 cut(s) 92
EcoRII CCWGG 1 cut(s) 964
EcoT14I CCWWGG 1 cut(s) 375
EcoT22I ATGCAT 3 cut(s) 190, 556, 870
ErhI CCWWGG 1 cut(s) 375
Esp3I CGTCTC 1 cut(s) 527
FaeI CATG 2 cut(s) 312, 379
FalI AAGNNNNNCTT 2 cut(s) 214, 246
FatI CATG 2 cut(s) 308, 375
FbaI TGATCA 2 cut(s) 384, 514
FblI GTMKAC 4 cut(s) 669, 1001, 1167, 1285
Fnu4HI GCNGC 5 cut(s) 20, 50, 525, 970, 1119
FokI GGATG 4 cut(s) 700, 805, 817, 900
Fsp4HI GCNGC 5 cut(s) 20, 50, 525, 970, 1119
FspBI CTAG 2 cut(s) 1044, 1238
GlaI GCGC 1 cut(s) 407
GluI GCNGC 5 cut(s) 20, 50, 525, 970, 1119
GsuI CTGGAG 1 cut(s) 551
HaeII RGCGCY 1 cut(s) 409
HaeIII GGCC 4 cut(s) 73, 380, 398, 969
HapII CCGG 1 cut(s) 403
HgaI GACGC 2 cut(s) 544, 1012
HhaI GCGC 1 cut(s) 408
Hin1II CATG 2 cut(s) 312, 379
Hin6I GCGC 1 cut(s) 406
HinP1I GCGC 1 cut(s) 406
HincII GTYRAC 2 cut(s) 1002, 1286
HindII GTYRAC 2 cut(s) 1002, 1286
HindIII AAGCTT 1 cut(s) 881
HinfI GANTC 7 cut(s) 64, 167, 193, 251, 761, 896, 1282
HpaII CCGG 1 cut(s) 403
HphI GGTGA 2 cut(s) 165, 761
Hpy166II GTNNAC 6 cut(s) 336, 582, 670, 1002, 1168, 1286
Hpy188I TCNGA 6 cut(s) 39, 118, 662, 1108, 1156, 1198
Hpy188III TCNNGA 7 cut(s) 80, 164, 222, 530, 847, 1091, 1279
Hpy8I GTNNAC 6 cut(s) 336, 582, 670, 1002, 1168, 1286
Hpy99I CGWCG 2 cut(s) 185, 1006
HpyAV CCTTC 4 cut(s) 123, 240, 778, 802
HpyCH4III ACNGT 4 cut(s) 131, 512, 931, 1102
HpyCH4IV ACGT 1 cut(s) 27
HpyF10VI GCNNNNNNNGC 3 cut(s) 19, 310, 709
HpyF3I CTNAG 2 cut(s) 680, 1038
HpySE526I ACGT 1 cut(s) 27
Hsp92II CATG 2 cut(s) 312, 379
HspAI GCGC 1 cut(s) 406
Ksp22I TGATCA 2 cut(s) 384, 514
Kzo9I GATC 6 cut(s) 384, 514, 849, 943, 1215, 1273
LmnI GCTCC 2 cut(s) 373, 732
Lsp1109I GCAGC 3 cut(s) 6, 36, 1105
LweI GCATC 4 cut(s) 175, 541, 852, 981
MaeI CTAG 2 cut(s) 1044, 1238
MaeII ACGT 1 cut(s) 27
MaeIII GTNAC 2 cut(s) 571, 922
MalI GATC 6 cut(s) 386, 516, 851, 945, 1217, 1275
MbiI CCGCTC 1 cut(s) 357
MboI GATC 6 cut(s) 384, 514, 849, 943, 1215, 1273
MboII GAAGA 6 cut(s) 54, 95, 162, 228, 231, 1078
MfeI CAATTG 1 cut(s) 585
MflI RGATCY 1 cut(s) 1215
MhlI GDGCHC 1 cut(s) 584
MlsI TGGCCA 2 cut(s) 73, 398
MluCI AATT 5 cut(s) 92, 325, 585, 718, 1226
MluNI TGGCCA 2 cut(s) 73, 398
MlyI GAGTC 4 cut(s) 187, 245, 770, 1291
MmeI TCCRAC 2 cut(s) 873, 1134
MnlI CCTC 8 cut(s) 171, 186, 359, 385, 723, 783, 789, 1033
Mox20I TGGCCA 2 cut(s) 73, 398
Mph1103I ATGCAT 3 cut(s) 190, 556, 870
MscI TGGCCA 2 cut(s) 73, 398
MseI TTAA 4 cut(s) 107, 507, 738, 780
MslI CAYNNNNRTG 1 cut(s) 380
Msp20I TGGCCA 2 cut(s) 73, 398
MspCI CTTAAG 1 cut(s) 737
MspI CCGG 1 cut(s) 403
MspR9I CCNGG 1 cut(s) 966
MunI CAATTG 1 cut(s) 585
MvaI CCWGG 1 cut(s) 966
MwoI GCNNNNNNNGC 3 cut(s) 19, 310, 709
NcoI CCATGG 1 cut(s) 375
NdeII GATC 6 cut(s) 384, 514, 849, 943, 1215, 1273
NlaIII CATG 2 cut(s) 312, 379
NlaIV GGNNCC 2 cut(s) 276, 734
NmuCI GTSAC 1 cut(s) 571
NsiI ATGCAT 3 cut(s) 190, 556, 870
PaeR7I CTCGAG 2 cut(s) 162, 1017
PfeI GAWTC 3 cut(s) 64, 167, 896
PkrI GCNGC 5 cut(s) 21, 51, 526, 971, 1120
Ple19I CGATCG 1 cut(s) 946
PleI GAGTC 4 cut(s) 187, 245, 769, 1290
PpsI GAGTC 4 cut(s) 187, 245, 769, 1290
Ppu21I YACGTR 1 cut(s) 28
PpuMI RGGWCCY 1 cut(s) 274
Psp5II RGGWCCY 1 cut(s) 274
Psp6I CCWGG 1 cut(s) 964
PspGI CCWGG 1 cut(s) 964
PspN4I GGNNCC 2 cut(s) 276, 734
PspPI GGNCC 3 cut(s) 143, 274, 336
PspPPI RGGWCCY 1 cut(s) 274
PspXI VCTCGAGB 1 cut(s) 1017
PstNI CAGNNNCTG 1 cut(s) 371
PsuI RGATCY 1 cut(s) 1215
PvuI CGATCG 1 cut(s) 946
RsaI GTAC 3 cut(s) 462, 560, 1031
RsaNI GTAC 3 cut(s) 461, 559, 1030
RseI CAYNNNNRTG 1 cut(s) 380
SalI GTCGAC 2 cut(s) 1000, 1284
SaqAI TTAA 4 cut(s) 107, 507, 738, 780
SatI GCNGC 5 cut(s) 20, 50, 525, 970, 1119
Sau3AI GATC 6 cut(s) 384, 514, 849, 943, 1215, 1273
Sau96I GGNCC 3 cut(s) 143, 274, 336
SchI GAGTC 4 cut(s) 187, 245, 770, 1291
ScrFI CCNGG 1 cut(s) 966
SduI GDGCHC 1 cut(s) 584
SfaNI GCATC 4 cut(s) 175, 541, 852, 981
Sfr274I CTCGAG 2 cut(s) 162, 1017
SinI GGWCC 3 cut(s) 143, 274, 336
SlaI CTCGAG 2 cut(s) 162, 1017
SmiMI CAYNNNNRTG 1 cut(s) 380
SmlI CTYRAG 5 cut(s) 162, 737, 1017, 1175, 1279
SmoI CTYRAG 5 cut(s) 162, 737, 1017, 1175, 1279
SnaBI TACGTA 1 cut(s) 28
Sse9I AATT 5 cut(s) 92, 325, 585, 718, 1226
SsiI CCGC 5 cut(s) 302, 355, 524, 970, 1254
SspMI CTAG 2 cut(s) 1044, 1238
StyD4I CCNGG 1 cut(s) 964
StyI CCWWGG 1 cut(s) 375
TaaI ACNGT 4 cut(s) 131, 512, 931, 1102
TaiI ACGT 1 cut(s) 30
TaqI TCGA 9 cut(s) 62, 163, 183, 359, 440, 635, 1001, 1018, 1285
TasI AATT 5 cut(s) 92, 325, 585, 718, 1226
TatI WGTACW 3 cut(s) 460, 558, 1029
TauI GCSGC 2 cut(s) 527, 972
TfiI GAWTC 3 cut(s) 64, 167, 896
Tru1I TTAA 4 cut(s) 107, 507, 738, 780
Tru9I TTAA 4 cut(s) 107, 507, 738, 780
TseFI GTSAC 1 cut(s) 571
TseI GCWGC 3 cut(s) 19, 49, 1118
Tsp45I GTSAC 1 cut(s) 571
TspDTI ATGAA 3 cut(s) 105, 821, 888
Vha464I CTTAAG 1 cut(s) 737
VneI GTGCAC 1 cut(s) 580
VpaK11BI GGWCC 3 cut(s) 143, 274, 336
XapI RAATTY 1 cut(s) 92
XhoI CTCGAG 2 cut(s) 162, 1017
XmiI GTMKAC 4 cut(s) 669, 1001, 1167, 1285
XspI CTAG 2 cut(s) 1044, 1238
Zsp2I ATGCAT 3 cut(s) 190, 556, 870
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.