pycom10g23000

Acetolactate synthase small subunit 1, chloroplastic-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0008290
Physical Location & Seq
Forward (+)
25251883 .. 25257137
5255 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1434 bp
ATGGCGGCTGTGTCATCACCATTCTCCACTTCACTAGTGGCCCAGAAACCGAGTTTCCCAGAGGATTTCCGGCATGGGTTTTCCACAAGCTTCGATCTTGGCCGCCAGCGGCCGCTCGTACGGAAGAGTTCCGAGCTGAAGAATCTGGTGGTCTCTGCTAGCACTGTCAATGCTGTTTCTGTCAGTGATTCTGTGCCTTCATTGACTTCTAAGGTGAAACGCCATACTATTTCAGTGTTTGTTGGGGATGAGAGTGGAATCATAAACCGAATAGCAGGGGTTTTCGCCAGGAGAGGTTACAATATCGAGTCTCTTGCTGTTGGTTTGAACAAGGACAAGGCTCTCTTTACCATAGTTGTGTCTGGAACTGAAAAGGTGTTGCGGCAGGTTGTTGAACAGCTCAACAAGTTGGTGAACGTGATAAACGTCGAAGACATCTCAAGGGAGCCACAAGTAGAACGTGAACTGATGCTTATCAAACTTAATGCAGATCCAAGTACCCGTGCAGAGATAATGTGGTTAGTGGACATCTTCAGAGCAAAAATTGTTGATATCTCTGAACAGTCACTGACAGTTGAGGTTACTGGGGACCCTGGGAAGTTGGTTGCTGTTCAAAGAAACTTAAGGAAGTTTGGAATCAAAGAACTTGCTAGAACTGGAAAGATTGCTTTGAGACGAGAAAAGATGGGTGCAACAGCTCCTTTCTGGAGATTTTCTGCAGATTCTTATCCAGATCTTAAGAAGTCCACATCTGATGGTGCTCTTGCAGTGAAAGCAAATCGTACGCTCAATGGTGATGCTGGTACTGCCTCAAGGGGTGACGTTTATCCTGTGGAGCCCTATGATGACTTCCGTTTGAATAAAGTTCTTGATGCGCATTGGGGTGTTCTCTACGATGAAGATTCAAGTGGCGTTCGATCACACACTTTGTCTATGATTGTAAATGACTATCCTGGAGTTCTAAACACAGTTACTGGAGTCCTGTCCCGAAGAGGTTATAACATTCAGAGCCTAGCTGTGGGGCCCGCTGAAATGGAGGGGCTTTCTCGCATTACAACAGTTGTTCCCGGTACAGATGATTCAATATCCAAGTTGGTTCAGCAACTCCACAAGTTGATAGATCTTCATGAGGTTCGGGATATTAGTCACTTACCATTTGCTGAGCGAGAGTTGATGTTGATAAAGATTGCTGTGAACACTACTGCTAGAAGGGATGTTCTTGATATTGCCAGCATTTTCCGTGCCAAAGCTGTTGATGTATCCGGTCATACAATTACCCTTGAGCTGACTGGTGATTTAAATAAGATGGTTGCACTGCAGAAGTTACTAGAGCCCTATGGGATTTGCGAGGTTGCGCGGACTGGGAGGGTGGCGTTGCAGCGGGAGTCTGGTGTGGATTCCACATATCTCCGCGGATACCCCCTTCAGTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00290 ko00650 ko00660 ko00770 ko01100 ko01110 ko01130 ko01210 ko01230 map00290 map00650 map00660 map00770 map01100 map01110 map01130 map01210 map01230
Pfam Domains
Protein Families
ACT ACT_5 ALS_ss_C

Protein Analysis

478

Amino Acids

52.44

Weight (kDa)

9.03

Isoelectric Point (pI)

38.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ACT PF01842 75 - 138 5.6e-11 ACT domain
ACT_AHAS_ss PF22629 77 - 141 9e-17 AHAS small subunit-like ACT domain
ACT_5 PF13710 85 - 143 2.2e-08 ACT domain
ALS_ss_C PF10369 154 - 227 9.8e-29 Small subunit of acetolactate synthase
ACT PF01842 308 - 373 4.2e-10 ACT domain
ACT_AHAS_ss PF22629 310 - 375 6.5e-22 AHAS small subunit-like ACT domain
ACT_5 PF13710 318 - 378 3.5e-10 ACT domain
ALS_ss_C PF10369 389 - 462 1.8e-27 Small subunit of acetolactate synthase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 999
Acc16I TGCGCA 1 cut(s) 876
Acc36I ACCTGC 1 cut(s) 376
AccBSI CCGCTC 1 cut(s) 115
AccII CGCG 2 cut(s) 1357, 1413
AclWI GGATC 1 cut(s) 485
AcoI YGGCCR 2 cut(s) 100, 110
AcuI CTGAAG 3 cut(s) 158, 517, 1409
AfaI GTAC 5 cut(s) 120, 499, 784, 805, 1072
AfiI CCNNNNNNNGG 1 cut(s) 1018
AflII CTTAAG 2 cut(s) 622, 737
AgsI TTSAA 6 cut(s) 328, 395, 614, 859, 906, 1083
AhlI ACTAGT 1 cut(s) 34
AjnI CCWGG 3 cut(s) 287, 592, 952
AluBI AGCT 7 cut(s) 90, 136, 400, 698, 1016, 1250, 1285
AluI AGCT 7 cut(s) 90, 136, 400, 698, 1016, 1250, 1285
Alw21I GWGCWC 1 cut(s) 763
Alw26I GTCTC 3 cut(s) 157, 315, 667
AlwI GGATC 1 cut(s) 485
AlwNI CAGNNNCTG 2 cut(s) 568, 974
AoxI GGCC 4 cut(s) 39, 100, 110, 1022
ApaI GGGCCC 1 cut(s) 1026
ApeKI GCWGC 1 cut(s) 1378
AspLEI GCGC 2 cut(s) 877, 1357
AspS9I GGNCC 4 cut(s) 40, 589, 1022, 1023
AsuC2I CCSGG 1 cut(s) 1068
AsuHPI GGTGA 6 cut(s) 9, 226, 424, 806, 830, 1304
AsuNHI GCTAGC 1 cut(s) 158
AvaII GGWCC 1 cut(s) 589
BaeGI GKGCMC 1 cut(s) 1026
BanII GRGCYC 3 cut(s) 840, 1026, 1335
BbsI GAAGAC 1 cut(s) 438
Bbv12I GWGCWC 1 cut(s) 763
BbvI GCAGC 1 cut(s) 1390
BccI CCATC 3 cut(s) 679, 749, 1300
BcgI CGANNNNNNTGC 2 cut(s) 296, 330
BciT130I CCWGG 3 cut(s) 289, 594, 954
BciVI GTATCC 2 cut(s) 1270, 1409
BcnI CCSGG 1 cut(s) 1068
BcoDI GTCTC 3 cut(s) 157, 315, 667
BcuI ACTAGT 1 cut(s) 34
BfaI CTAG 6 cut(s) 35, 159, 651, 1013, 1206, 1328
BfmI CTRYAG 2 cut(s) 717, 1316
BfrI CTTAAG 2 cut(s) 622, 737
BfuAI ACCTGC 1 cut(s) 376
BfuI GTATCC 2 cut(s) 1270, 1409
BglII AGATCT 2 cut(s) 733, 1120
BisI GCNGC 6 cut(s) 6, 103, 110, 113, 383, 1379
BlpI GCTNAGC 1 cut(s) 1161
BlsI GCNGC 6 cut(s) 7, 104, 111, 114, 384, 1380
Bme1390I CCNGG 4 cut(s) 289, 594, 954, 1068
Bme18I GGWCC 1 cut(s) 589
BmgT120I GGNCC 4 cut(s) 40, 589, 1022, 1023
BmiI GGNNCC 6 cut(s) 447, 590, 591, 837, 1023, 1024
BmrFI CCNGG 4 cut(s) 289, 594, 954, 1068
BmrI ACTGGG 2 cut(s) 594, 1371
BmsI GCATC 3 cut(s) 459, 787, 862
BmtI GCTAGC 1 cut(s) 162
BmuI ACTGGG 2 cut(s) 594, 1371
BpiI GAAGAC 1 cut(s) 438
BpmI CTGGAG 3 cut(s) 727, 975, 996
Bpu1102I GCTNAGC 1 cut(s) 1161
BpuEI CTTGAG 3 cut(s) 424, 796, 1301
BpuMI CCSGG 1 cut(s) 1068
BsaBI GATNNNNATC 2 cut(s) 473, 726
BsaI GGTCTC 1 cut(s) 157
BsaJI CCNNGG 3 cut(s) 592, 593, 1411
BsaWI WCCGGW 1 cut(s) 1262
Bsc4I CCNNNNNNNGG 1 cut(s) 1018
Bse1I ACTGG 5 cut(s) 589, 661, 979, 1294, 1366
Bse8I GATNNNNATC 2 cut(s) 473, 726
BseBI CCWGG 3 cut(s) 289, 594, 954
BseDI CCNNGG 3 cut(s) 592, 593, 1411
BseGI GGATG 2 cut(s) 253, 1219
BseJI GATNNNNATC 2 cut(s) 473, 726
BseLI CCNNNNNNNGG 1 cut(s) 1018
BseMII CTCAG 1 cut(s) 1152
BseNI ACTGG 5 cut(s) 589, 661, 979, 1294, 1366
BseSI GKGCMC 1 cut(s) 1026
BseX3I CGGCCG 1 cut(s) 110
BseXI GCAGC 1 cut(s) 1390
BsgI GTGCAG 1 cut(s) 525
Bsh1236I CGCG 2 cut(s) 1357, 1413
Bsh1285I CGRYCG 1 cut(s) 113
BshFI GGCC 4 cut(s) 41, 102, 112, 1024
BsiEI CGRYCG 1 cut(s) 113
BsiHKAI GWGCWC 1 cut(s) 763
BsiSI CCGG 3 cut(s) 70, 1068, 1263
BsiWI CGTACG 2 cut(s) 118, 782
BslFI GGGAC 2 cut(s) 602, 970
BslI CCNNNNNNNGG 1 cut(s) 1018
BsmAI GTCTC 3 cut(s) 157, 315, 667
BsmBI CGTCTC 1 cut(s) 667
BsmFI GGGAC 2 cut(s) 602, 970
BsnI GGCC 4 cut(s) 41, 102, 112, 1024
Bso31I GGTCTC 1 cut(s) 157
Bsp120I GGGCCC 1 cut(s) 1022
Bsp1286I GDGCHC 4 cut(s) 763, 840, 1026, 1335
Bsp143I GATC 5 cut(s) 94, 490, 733, 917, 1120
Bsp1720I GCTNAGC 1 cut(s) 1161
BspANI GGCC 4 cut(s) 41, 102, 112, 1024
BspCNI CTCAG 1 cut(s) 1153
BspFNI CGCG 2 cut(s) 1357, 1413
BspHI TCATGA 1 cut(s) 1126
BspLI GGNNCC 6 cut(s) 447, 590, 591, 837, 1023, 1024
BspMAI CTGCAG 2 cut(s) 721, 1320
BspMI ACCTGC 1 cut(s) 376
BspOI GCTAGC 1 cut(s) 162
BspPI GGATC 1 cut(s) 485
BspTI CTTAAG 2 cut(s) 622, 737
BspTNI GGTCTC 1 cut(s) 157
BsrBI CCGCTC 1 cut(s) 115
BsrI ACTGG 5 cut(s) 589, 661, 979, 1294, 1366
BssECI CCNNGG 3 cut(s) 592, 593, 1411
BssMI GATC 5 cut(s) 94, 490, 733, 917, 1120
Bst2UI CCWGG 3 cut(s) 289, 594, 954
Bst4CI ACNGT 5 cut(s) 166, 564, 574, 970, 1060
Bst6I CTCTTC 2 cut(s) 119, 985
BstAFI CTTAAG 2 cut(s) 622, 737
BstC8I GCNNGC 4 cut(s) 107, 160, 1026, 1231
BstDEI CTNAG 2 cut(s) 210, 1161
BstDSI CCRYGG 1 cut(s) 1411
BstF5I GGATG 2 cut(s) 253, 1219
BstFNI CGCG 2 cut(s) 1357, 1413
BstHHI GCGC 2 cut(s) 877, 1357
BstKTI GATC 5 cut(s) 97, 493, 736, 920, 1123
BstMAI GTCTC 3 cut(s) 157, 315, 667
BstMBI GATC 5 cut(s) 94, 490, 733, 917, 1120
BstMCI CGRYCG 1 cut(s) 113
BstMWI GCNNNNNNNGC 2 cut(s) 773, 806
BstNI CCWGG 3 cut(s) 289, 594, 954
BstSCI CCNGG 4 cut(s) 287, 592, 952, 1066
BstSFI CTRYAG 2 cut(s) 717, 1316
BstSLI GKGCMC 1 cut(s) 1026
BstUI CGCG 2 cut(s) 1357, 1413
BstV1I GCAGC 1 cut(s) 1390
BstV2I GAAGAC 1 cut(s) 438
BstX2I RGATCY 3 cut(s) 490, 733, 1120
BstYI RGATCY 3 cut(s) 490, 733, 1120
BstZI CGGCCG 1 cut(s) 110
BsuI GTATCC 2 cut(s) 1270, 1409
BsuRI GGCC 4 cut(s) 41, 102, 112, 1024
BtgI CCRYGG 1 cut(s) 1411
BtsCI GGATG 2 cut(s) 253, 1219
BtsI GCAGTG 2 cut(s) 774, 1313
BtsIMutI CAGTG 6 cut(s) 162, 190, 240, 566, 774, 1313
BveI ACCTGC 1 cut(s) 376
Cac8I GCNNGC 4 cut(s) 107, 160, 1026, 1231
CaiI CAGNNNCTG 2 cut(s) 568, 974
CciI TCATGA 1 cut(s) 1126
CciNI GCGGCCGC 1 cut(s) 110
CfoI GCGC 2 cut(s) 877, 1357
Cfr13I GGNCC 4 cut(s) 40, 589, 1022, 1023
Cfr42I CCGCGG 1 cut(s) 1414
Csp6I GTAC 5 cut(s) 119, 498, 783, 804, 1071
CviAII CATG 2 cut(s) 74, 1127
CviQI GTAC 5 cut(s) 119, 498, 783, 804, 1071
DdeI CTNAG 2 cut(s) 210, 1161
DpnI GATC 5 cut(s) 96, 492, 735, 919, 1122
DpnII GATC 5 cut(s) 94, 490, 733, 917, 1120
DraI TTTAAA 1 cut(s) 1299
EaeI YGGCCR 2 cut(s) 100, 110
EagI CGGCCG 1 cut(s) 110
Eam1104I CTCTTC 2 cut(s) 119, 985
EarI CTCTTC 2 cut(s) 119, 985
EclXI CGGCCG 1 cut(s) 110
Eco24I GRGCYC 3 cut(s) 840, 1026, 1335
Eco31I GGTCTC 1 cut(s) 157
Eco32I GATATC 1 cut(s) 553
Eco47I GGWCC 1 cut(s) 589
Eco52I CGGCCG 1 cut(s) 110
Eco57I CTGAAG 3 cut(s) 158, 517, 1409
EcoO109I RGGNCCY 2 cut(s) 589, 1022
EcoRII CCWGG 3 cut(s) 287, 592, 952
EcoRV GATATC 1 cut(s) 553
EcoT38I GRGCYC 3 cut(s) 840, 1026, 1335
Esp3I CGTCTC 1 cut(s) 667
FaeI CATG 2 cut(s) 77, 1130
FalI AAGNNNNNCTT 2 cut(s) 329, 361
FaqI GGGAC 2 cut(s) 602, 970
FatI CATG 2 cut(s) 73, 1126
FauI CCCGC 2 cut(s) 1033, 1374
Fnu4HI GCNGC 6 cut(s) 6, 103, 110, 113, 383, 1379
FokI GGATG 2 cut(s) 260, 1226
FriOI GRGCYC 3 cut(s) 840, 1026, 1335
Fsp4HI GCNGC 6 cut(s) 6, 103, 110, 113, 383, 1379
FspAI RTGCGCAY 1 cut(s) 876
FspBI CTAG 6 cut(s) 35, 159, 651, 1013, 1206, 1328
FspI TGCGCA 1 cut(s) 876
GlaI GCGC 2 cut(s) 876, 1356
GluI GCNGC 6 cut(s) 6, 103, 110, 113, 383, 1379
GsuI CTGGAG 3 cut(s) 727, 975, 996
HaeIII GGCC 4 cut(s) 41, 102, 112, 1024
HapII CCGG 3 cut(s) 70, 1068, 1263
HhaI GCGC 2 cut(s) 877, 1357
Hin1II CATG 2 cut(s) 77, 1130
Hin6I GCGC 2 cut(s) 875, 1355
HinP1I GCGC 2 cut(s) 875, 1355
HindIII AAGCTT 1 cut(s) 88
HpaII CCGG 3 cut(s) 70, 1068, 1263
HphI GGTGA 6 cut(s) 9, 226, 424, 806, 830, 1304
Hpy166II GTNNAC 5 cut(s) 415, 464, 526, 747, 1195
Hpy188I TCNGA 5 cut(s) 133, 536, 559, 754, 1008
Hpy188III TCNNGA 8 cut(s) 363, 706, 731, 869, 987, 1127, 1136, 1220
Hpy8I GTNNAC 5 cut(s) 415, 464, 526, 747, 1195
Hpy99I CGWCG 1 cut(s) 431
HpyAV CCTTC 3 cut(s) 207, 1203, 1433
HpyCH4III ACNGT 5 cut(s) 166, 564, 574, 970, 1060
HpyCH4IV ACGT 4 cut(s) 417, 426, 460, 822
HpyCH4V TGCA 8 cut(s) 488, 506, 692, 719, 767, 1313, 1318, 1378
HpyF10VI GCNNNNNNNGC 2 cut(s) 773, 806
HpyF3I CTNAG 2 cut(s) 210, 1161
HpySE526I ACGT 4 cut(s) 417, 426, 460, 822
Hsp92II CATG 2 cut(s) 77, 1130
HspAI GCGC 2 cut(s) 875, 1355
KflI GGGWCCC 1 cut(s) 589
KspI CCGCGG 1 cut(s) 1414
Kzo9I GATC 5 cut(s) 94, 490, 733, 917, 1120
LmnI GCTCC 3 cut(s) 445, 703, 835
Lsp1109I GCAGC 1 cut(s) 1390
LweI GCATC 3 cut(s) 459, 787, 862
MaeI CTAG 6 cut(s) 35, 159, 651, 1013, 1206, 1328
MaeII ACGT 4 cut(s) 417, 426, 460, 822
MaeIII GTNAC 7 cut(s) 296, 564, 580, 818, 970, 1145, 1323
MalI GATC 5 cut(s) 96, 492, 735, 919, 1122
MbiI CCGCTC 1 cut(s) 115
MboI GATC 5 cut(s) 94, 490, 733, 917, 1120
MboII GAAGA 7 cut(s) 136, 151, 443, 523, 911, 1002, 1115
MflI RGATCY 3 cut(s) 490, 733, 1120
MhlI GDGCHC 4 cut(s) 763, 840, 1026, 1335
MluCI AATT 2 cut(s) 543, 1272
MlyI GAGTC 3 cut(s) 317, 987, 1394
MnlI CCTC 9 cut(s) 55, 287, 571, 820, 986, 1030, 1123, 1342, 1359
MseI TTAA 4 cut(s) 483, 623, 738, 1298
MslI CAYNNNNRTG 2 cut(s) 356, 882
MspA1I CMGCKG 4 cut(s) 109, 1028, 1381, 1413
MspCI CTTAAG 2 cut(s) 622, 737
MspI CCGG 3 cut(s) 70, 1068, 1263
MspR9I CCNGG 4 cut(s) 289, 594, 954, 1068
MvaI CCWGG 3 cut(s) 289, 594, 954
MvnI CGCG 2 cut(s) 1357, 1413
MwoI GCNNNNNNNGC 2 cut(s) 773, 806
NciI CCSGG 1 cut(s) 1068
NdeII GATC 5 cut(s) 94, 490, 733, 917, 1120
NheI GCTAGC 1 cut(s) 158
NlaIII CATG 2 cut(s) 77, 1130
NlaIV GGNNCC 6 cut(s) 447, 590, 591, 837, 1023, 1024
NmuCI GTSAC 3 cut(s) 564, 818, 1145
NotI GCGGCCGC 1 cut(s) 110
NsbI TGCGCA 1 cut(s) 876
PagI TCATGA 1 cut(s) 1126
PasI CCCWGGG 1 cut(s) 593
PcsI WCGNNNNNNNCGW 1 cut(s) 423
PfeI GAWTC 8 cut(s) 142, 188, 258, 636, 722, 902, 1079, 1397
Pfl23II CGTACG 2 cut(s) 118, 782
PfoI TCCNGGA 1 cut(s) 952
PkrI GCNGC 6 cut(s) 7, 104, 111, 114, 384, 1380
PleI GAGTC 3 cut(s) 316, 986, 1393
PpsI GAGTC 3 cut(s) 316, 986, 1393
PpuMI RGGWCCY 1 cut(s) 589
PsiI TTATAA 1 cut(s) 999
Psp5II RGGWCCY 1 cut(s) 589
Psp6I CCWGG 3 cut(s) 287, 592, 952
PspGI CCWGG 3 cut(s) 287, 592, 952
PspLI CGTACG 2 cut(s) 118, 782
PspN4I GGNNCC 6 cut(s) 447, 590, 591, 837, 1023, 1024
PspOMI GGGCCC 1 cut(s) 1022
PspPI GGNCC 4 cut(s) 40, 589, 1022, 1023
PspPPI RGGWCCY 1 cut(s) 589
PstI CTGCAG 2 cut(s) 721, 1320
PstNI CAGNNNCTG 2 cut(s) 568, 974
PsuI RGATCY 3 cut(s) 490, 733, 1120
RsaI GTAC 5 cut(s) 120, 499, 784, 805, 1072
RsaNI GTAC 5 cut(s) 119, 498, 783, 804, 1071
RseI CAYNNNNRTG 2 cut(s) 356, 882
SacII CCGCGG 1 cut(s) 1414
SaqAI TTAA 4 cut(s) 483, 623, 738, 1298
SatI GCNGC 6 cut(s) 6, 103, 110, 113, 383, 1379
Sau3AI GATC 5 cut(s) 94, 490, 733, 917, 1120
Sau96I GGNCC 4 cut(s) 40, 589, 1022, 1023
SchI GAGTC 3 cut(s) 317, 987, 1394
ScrFI CCNGG 4 cut(s) 289, 594, 954, 1068
SduI GDGCHC 4 cut(s) 763, 840, 1026, 1335
SfaNI GCATC 3 cut(s) 459, 787, 862
SfcI CTRYAG 2 cut(s) 717, 1316
Sfr303I CCGCGG 1 cut(s) 1414
SgrBI CCGCGG 1 cut(s) 1414
SinI GGWCC 1 cut(s) 589
SmiI ATTTAAAT 1 cut(s) 1299
SmiMI CAYNNNNRTG 2 cut(s) 356, 882
SmlI CTYRAG 5 cut(s) 439, 622, 737, 811, 1280
SmoI CTYRAG 5 cut(s) 439, 622, 737, 811, 1280
SpeI ACTAGT 1 cut(s) 34
Sse9I AATT 2 cut(s) 543, 1272
SspMI CTAG 6 cut(s) 35, 159, 651, 1013, 1206, 1328
StyD4I CCNGG 4 cut(s) 287, 592, 952, 1066
SwaI ATTTAAAT 1 cut(s) 1299
TaaI ACNGT 5 cut(s) 166, 564, 574, 970, 1060
TaiI ACGT 4 cut(s) 420, 429, 463, 825
TaqI TCGA 4 cut(s) 93, 306, 429, 916
TasI AATT 2 cut(s) 543, 1272
TauI GCSGC 5 cut(s) 8, 105, 112, 115, 385
TfiI GAWTC 8 cut(s) 142, 188, 258, 636, 722, 902, 1079, 1397
Tru1I TTAA 4 cut(s) 483, 623, 738, 1298
Tru9I TTAA 4 cut(s) 483, 623, 738, 1298
TscAI CASTG 6 cut(s) 169, 190, 240, 573, 774, 1320
TseFI GTSAC 3 cut(s) 564, 818, 1145
TseI GCWGC 1 cut(s) 1378
Tsp45I GTSAC 3 cut(s) 564, 818, 1145
TspDTI ATGAA 3 cut(s) 189, 912, 1115
TspGWI ACGGA 3 cut(s) 136, 842, 1229
TspRI CASTG 6 cut(s) 169, 190, 240, 573, 774, 1320
Vha464I CTTAAG 2 cut(s) 622, 737
VpaK11BI GGWCC 1 cut(s) 589
XcmI CCANNNNNNNNNTGG 1 cut(s) 34
XspI CTAG 6 cut(s) 35, 159, 651, 1013, 1206, 1328
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.