Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr10

Orthogroup ID

OG0012217

Physical Location & Seq

Genomic Coordinates Reverse (-)

25602889 .. 25607641

Gene Structure 4753 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2430 bp

ATGTCATTGAGAAGGAACTCCAAACCAACACAACGAGGTGTCTTCTTCTTCTTCTTCTTATTATATTCGAACTTGATCTCTTCACTCTTTCTGACAAACAATTTATATACTTTTACCCTTTGTACTCTTCAATTCTACGAAATCTTACCCCCCAAAAAAGAAGAGGGCAAGTTCGCAGCTAATCGCAGCAGAAAAATCATGGAGGGAGGCAGCGGGAAATCCATACTCCAATTCGCGCCGTTTCAGAGCTCCGTCGACGAGGGTTTCTGGCACAGACTCTCTTCCTTGAAGCTCAACAAGTTCGGCATCGACGACTCGCCTGTTCCCATCACCGGTTTCTACGCACCGTGCTCGCATTCTCAAGTGTCAAATCATTTAACTCTTTTAGCCGAGTCTTTGCCTTCGGATTCGAGTGAGGAATCATCGGTACCAGGAGTAAGTCGGGGCAATAGGAACAGATGCTCCGTTCCAGGAATCATTTACAACACCAATACGGTAGAGGGCTTTCATGCGCTTGATAAACAGGGCTTGCTTAAGGCAGAAGCGCAAAAGATTTGGGAGGACATCCAGAGTGGAAGAGCATTGGAGGATAGTTCAGTACTCTCGAGGTTCCTCGTCATCTCTTTTGCAGACCTGAAAAAATGGAGCTTTAATTACTGGTTTGCTTTCCCCGCTCTGGTGCTTGATCCTCCAGCAACTTTGGTAAATTTGAGGCCAGCTTCAGAGTGTTTTAGCTTGGAGGAGGCAGAATCTGTTTCAGCAGCATTTAACGAGTGGCGTAACTCAAGCCTAACAGCAGACGTGCCATTCTTCTTGGTTCATATTGATTCAAATTCACACTCTACTATTAAGCATCTAAAGGATTGGGAAACCTCCCAAAGTGCTGATGATGGTCACAAGTTACTCTTTGGTTTTTACGATCCATGCCATCTTCCAAACCATCCCGGTTGGCCTCTTCGCAACCTCCTTGCTCTTATTTGCTCAAGATGGGATCTAAAGTCTGTTCACTTTTTATGCTATAGAGAGAGTCGGGGTTTCGCAGATCTGGGACTGTCCCTTGTTGGTGAAGCTCTGATTACAGTTCCGCCAGGATGGAGAGATCATGGTCATGTACCAAATGCAGTCGGGTGGGAACTTAACAAAGGGAGAAAAGTACCCAGGATTATTAGCCTTGCTAAATCGATCGATCCAACTAGGTTGGCCATATCTGCTGCAGATTTGAATTTGAAACTAATGAGATGGCGTGCTTTGCCTTCTTTGAACTTAAATAGCTTGTCTTCTCTCAAGTGTCTCCTCCTTGGAGCTGGTACACTTGGATGCCAGGTCGCTCGGACGCTTATGGCTTGGGGTGTCCGGAACATTACGTTAGTTGACAATGGCAGGGTGGCTATGTCTAATCCGCTGAGGCAATCACTGTATACATTAGATGACTGCCTAAATGGTGGTGAATATAAAGTGACAGCTGCAGTTAATAGTCTCAAGAAAATATTTCCAGCTGTGGAAGCAGAGGGTGTCATCATGGCTATACCGATGCCTGGACATCCTATTCCGAGTCAAGAAGAGCAGAGCGTGCTCGATGATTGTAGACGACTACATGATTTAATTGATTCTCACGATGTAGTTTTCCTGCTGACTGATACAAGAGAAAGTCGGTGGCTACCATCTCTCCTTTGTGCAAATACTAACAAGATTACTATAACTGCGGCTTTAGGGTTTGATAGCTTCTTGGTTATGCGCCATGGAGCAGGTCCTTGTAGCTCTAGCCATTACTCAAAAACTGAAGCTGAAAATGCTTTATCTGCTTATATGGGCAATCTTGGCCTTACTGATAGAGACGGGGGGAAGAGATTGGGCTGTTACTTCTGCAATGATGTTGTTGCGCCTATTGATTCAACTTCAAACCGCACATTGGACCAGCAATGCACTGTTACACGCCCAGGCCTTGCTCCTATTGCCTCAGCTCTTGCAGTTGAGCTCTTAGTGGGGATCCTGCATCACCCTCATGGGATATTTGCGGAAGGTGAGGTGGTAAACTCCAGTAATAGCGGAAGCAGTGAGCAGCCTCTTGGTATTTTACCCCATCAAATTCGCGGTTCCCTAGCACAGTTTTCGCAGATGACACTTGTGGGCCACTCCTCGGACAGTTGCACGGCTTGCTGCAGCACTGTGGTGTCCGAATATCGGAAAAGGGGAATGGAATTTATTTTGCAAGCAATCAACCATCCTACATATCTGGAGGACCTAACTGGACTGACAGAGTTGATGAAATCAGCTAGTAAATTCGAGTTGGACTGGGACGACGGGACAGATGAAGACGATGATGATTTGGTTGAGTTTGAACGGGGCGACGACTATGAACCTGAGAACCAGCCTTCCCGTGTCCTTGAAGCACGTTGTGCCGCCGCTGCCGCCTTGAATCACAAACCTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000045 GO:0000407 GO:0000422 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006464 GO:0006497 GO:0006501 GO:0006807 GO:0006914 GO:0006950 GO:0006952 GO:0006995 GO:0006996 GO:0007005 GO:0007033 GO:0007154 GO:0007275 GO:0007568 GO:0008150 GO:0008152 GO:0008641 GO:0009056 GO:0009058 GO:0009059 GO:0009267 GO:0009605 GO:0009607 GO:0009620 GO:0009987 GO:0009991 GO:0010150 GO:0016043 GO:0016236 GO:0016237 GO:0016740 GO:0016874 GO:0016877 GO:0018410 GO:0019538 GO:0019778 GO:0019779 GO:0022411 GO:0022607 GO:0031667 GO:0031668 GO:0031669 GO:0032501 GO:0032502 GO:0033554 GO:0034645 GO:0034727 GO:0036211 GO:0042157 GO:0042158 GO:0042594 GO:0043170 GO:0043207 GO:0043412 GO:0043562 GO:0043687 GO:0044085 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0044804 GO:0044805 GO:0048366 GO:0048367 GO:0048731 GO:0048827 GO:0048856 GO:0050832 GO:0050896 GO:0051704 GO:0051707 GO:0051716 GO:0061726 GO:0061919 GO:0070925 GO:0071496 GO:0071704 GO:0071840 GO:0090693 GO:0098542 GO:0099402 GO:1901564 GO:1901566 GO:1901576 GO:1903008 GO:1905037

KEGG Pathways

Metabolic & Signaling

ko04136 ko04138 ko04140 ko04216 map04136 map04138 map04140 map04216

Pfam Domains

Protein Families

ATG7_N ThiF

Protein Analysis

810

Amino Acids

89.26

Weight (kDa)

5.83

Isoelectric Point (pI)

47.65

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ATG7_N	PF16420	76 - 394	3.7e-101	Ubiquitin-like modifier-activating enzyme ATG7 N-terminus
ThiF	PF00899	412 - 667	1.4e-37	ThiF family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0012217)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G45900
fragaria_vesca	FvH4_3g07910
malus_domestica	MD05G1302300.v1.1 MD10G1281000.v1.1
prunus_persica	Prupe.4G060100_v2.0.a1 Prupe.4G060100_v2.0.a1 Prupe.4G060100_v2.0.a1
pyrus_communis	pycom05g27990 pycom10g23460
rosa_chinensis	RchiOBHm_Chr5g0010641
rosa_laevigata	RLG00000031768
rosa_multiflora	Rmu_sc0006103.1_g000002 Rmu_sc0014635.1_g000003
rosa_roxburghii	Rroxscaffold_1G00065730
rosa_rugosa	Rorug04G0451400.1
rosa_samantha	Rh5AG083000 Rh5CG091000
rosa_wichuraiana	Rw0G000680 Rw0G021850

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	1736
Acc65I	GGTACC	1 cut(s)	427
AccB1I	GGYRCC	1 cut(s)	427
AccBSI	CCGCTC	1 cut(s)	674
AccI	GTMKAC	3 cut(s)	255, 1418, 1585
AccII	CGCG	2 cut(s)	236, 2091
AccIII	TCCGGA	1 cut(s)	1353
AclWI	GGATC	6 cut(s)	680, 914, 999, 1181, 1981, 1994
AcoI	YGGCCR	1 cut(s)	1200
AcsI	RAATTY	6 cut(s)	706, 832, 1222, 2085, 2198, 2279
AcuI	CTGAAG	2 cut(s)	705, 1800
AdeI	CACNNNGTG	1 cut(s)	2396
AfaI	GTAC	6 cut(s)	124, 429, 600, 1113, 1155, 1309
AfiI	CCNNNNNNNGG	6 cut(s)	332, 676, 1535, 1909, 2137, 2181
AflII	CTTAAG	1 cut(s)	533
AgeI	ACCGGT	1 cut(s)	332
AjiI	CACGTC	1 cut(s)	802
AjnI	CCWGG	7 cut(s)	430, 469, 1087, 1157, 1320, 1534, 1936
AleI	CACNNNNGTG	1 cut(s)	2168
AloI	GAACNNNNNNTCC	4 cut(s)	446, 478, 2357, 2389
Alw21I	GWGCWC	4 cut(s)	251, 353, 1575, 1977
Alw26I	GTCTC	3 cut(s)	1295, 1481, 1827
AlwI	GGATC	6 cut(s)	680, 914, 999, 1181, 1981, 1994
AlwNI	CAGNNNCTG	1 cut(s)	752
Ama87I	CYCGRG	1 cut(s)	604
Aor13HI	TCCGGA	1 cut(s)	1353
AoxI	GGCC	6 cut(s)	713, 950, 1200, 1819, 1939, 2128
ApoI	RAATTY	6 cut(s)	706, 832, 1222, 2085, 2198, 2279
AsiGI	ACCGGT	1 cut(s)	332
Asp718I	GGTACC	1 cut(s)	427
AspLEI	GCGC	5 cut(s)	238, 514, 547, 1737, 1882
AspS9I	GGNCC	4 cut(s)	1748, 1912, 2128, 2239
AsuC2I	CCSGG	1 cut(s)	945
AsuHPI	GGTGA	5 cut(s)	322, 1076, 1457, 1988, 2033
AsuII	TTCGAA	1 cut(s)	68
AvaI	CYCGRG	1 cut(s)	604
AvaII	GGWCC	3 cut(s)	1748, 1912, 2239
BalI	TGGCCA	1 cut(s)	1202
BamHI	GGATCC	1 cut(s)	1986
BanI	GGYRCC	1 cut(s)	427
BanII	GRGCYC	2 cut(s)	251, 1977
BbsI	GAAGAC	3 cut(s)	34, 1269, 2319
Bbv12I	GWGCWC	4 cut(s)	251, 353, 1575, 1977
BbvCI	CCTCAGC	2 cut(s)	1403, 1957
BceAI	ACGGC	2 cut(s)	223, 2166
BcgI	CGANNNNNNTGC	4 cut(s)	333, 367, 2091, 2125
BciT130I	CCWGG	7 cut(s)	432, 471, 1089, 1159, 1322, 1536, 1938
BcnI	CCSGG	1 cut(s)	945
BcoDI	GTCTC	3 cut(s)	1295, 1481, 1827
BfaI	CTAG	4 cut(s)	1194, 1761, 2099, 2274
BfmI	CTRYAG	4 cut(s)	1018, 1212, 1464, 2158
BfrI	CTTAAG	1 cut(s)	533
BfuAI	ACCTGC	1 cut(s)	1736
BglII	AGATCT	1 cut(s)	1042
BmcAI	AGTACT	1 cut(s)	600
Bme1390I	CCNGG	8 cut(s)	432, 471, 945, 1089, 1159, 1322, 1536, 1938
Bme18I	GGWCC	3 cut(s)	1748, 1912, 2239
BmeT110I	CYCGRG	1 cut(s)	604
BmgBI	CACGTC	1 cut(s)	802
BmgT120I	GGNCC	4 cut(s)	1748, 1912, 2128, 2239
BmiI	GGNNCC	4 cut(s)	429, 611, 1988, 2095
BmrFI	CCNGG	8 cut(s)	432, 471, 945, 1089, 1159, 1322, 1536, 1938
BmrI	ACTGGG	1 cut(s)	2302
BmsI	GCATC	6 cut(s)	315, 449, 862, 1307, 1521, 2002
BmuI	ACTGGG	1 cut(s)	2302
BpiI	GAAGAC	3 cut(s)	34, 1269, 2319
BpmI	CTGGAG	3 cut(s)	675, 2020, 2255
Bpu10I	CCTNAGC	2 cut(s)	1403, 1957
Bpu14I	TTCGAA	1 cut(s)	68
BpuEI	CTTGAG	5 cut(s)	345, 769, 967, 1268, 1463
BpuMI	CCSGG	1 cut(s)	945
Bsa29I	ATCGAT	2 cut(s)	1181, 1185
BsaJI	CCNNGG	5 cut(s)	1157, 1297, 1738, 1936, 2136
BsaWI	WCCGGW	2 cut(s)	332, 1353
BsaXI	ACNNNNNCTCC	4 cut(s)	446, 476, 1650, 1680
Bsc4I	CCNNNNNNNGG	6 cut(s)	332, 676, 1535, 1909, 2137, 2181
Bse118I	RCCGGY	1 cut(s)	332
Bse1I	ACTGG	4 cut(s)	662, 2037, 2251, 2297
Bse3DI	GCAATG	2 cut(s)	1873, 1925
BseAI	TCCGGA	1 cut(s)	1353
BseBI	CCWGG	7 cut(s)	432, 471, 1089, 1159, 1322, 1536, 1938
BseCI	ATCGAT	2 cut(s)	1181, 1185
BseDI	CCNNGG	5 cut(s)	1157, 1297, 1738, 1936, 2136
BseGI	GGATG	6 cut(s)	564, 940, 1097, 1322, 1540, 2221
BseLI	CCNNNNNNNGG	6 cut(s)	332, 676, 1535, 1909, 2137, 2181
BseMI	GCAATG	2 cut(s)	1873, 1925
BseMII	CTCAG	3 cut(s)	1394, 1971, 2352
BseNI	ACTGG	4 cut(s)	662, 2037, 2251, 2297
BseRI	GAGGAG	3 cut(s)	755, 1283, 2125
Bsh1236I	CGCG	2 cut(s)	236, 2091
Bsh1285I	CGRYCG	1 cut(s)	1185
BshFI	GGCC	6 cut(s)	715, 952, 1202, 1821, 1941, 2130
BshNI	GGYRCC	1 cut(s)	427
BshTI	ACCGGT	1 cut(s)	332
BshVI	ATCGAT	2 cut(s)	1181, 1185
BsiEI	CGRYCG	1 cut(s)	1185
BsiHKAI	GWGCWC	4 cut(s)	251, 353, 1575, 1977
BsiHKCI	CYCGRG	1 cut(s)	604
BsiSI	CCGG	3 cut(s)	333, 945, 1354
BslFI	GGGAC	4 cut(s)	1039, 1062, 2309, 2317
BslI	CCNNNNNNNGG	6 cut(s)	332, 676, 1535, 1909, 2137, 2181
BsmAI	GTCTC	3 cut(s)	1295, 1481, 1827
BsmBI	CGTCTC	1 cut(s)	1827
BsmFI	GGGAC	4 cut(s)	1039, 1062, 2309, 2317
BsmI	GAATGC	1 cut(s)	355
BsnI	GGCC	6 cut(s)	715, 952, 1202, 1821, 1941, 2130
BsoBI	CYCGRG	1 cut(s)	604
Bsp119I	TTCGAA	1 cut(s)	68
Bsp1286I	GDGCHC	4 cut(s)	251, 353, 1575, 1977
Bsp13I	TCCGGA	1 cut(s)	1353
Bsp143I	GATC	9 cut(s)	75, 685, 919, 991, 1042, 1099, 1182, 1186, 1986
Bsp19I	CCATGG	1 cut(s)	1738
BspANI	GGCC	6 cut(s)	715, 952, 1202, 1821, 1941, 2130
BspCNI	CTCAG	3 cut(s)	1395, 1970, 2353
BspDI	ATCGAT	2 cut(s)	1181, 1185
BspEI	TCCGGA	1 cut(s)	1353
BspFNI	CGCG	2 cut(s)	236, 2091
BspLI	GGNNCC	4 cut(s)	429, 611, 1988, 2095
BspMAI	CTGCAG	3 cut(s)	1216, 1468, 2162
BspMI	ACCTGC	1 cut(s)	1736
BspPI	GGATC	6 cut(s)	680, 914, 999, 1181, 1981, 1994
BspQI	GCTCTTC	2 cut(s)	571, 1554
BspT104I	TTCGAA	1 cut(s)	68
BspT107I	GGYRCC	1 cut(s)	427
BspTI	CTTAAG	1 cut(s)	533
BsrBI	CCGCTC	1 cut(s)	674
BsrDI	GCAATG	2 cut(s)	1873, 1925
BsrFI	RCCGGY	1 cut(s)	332
BsrI	ACTGG	4 cut(s)	662, 2037, 2251, 2297
BssAI	RCCGGY	1 cut(s)	332
BssECI	CCNNGG	5 cut(s)	1157, 1297, 1738, 1936, 2136
BssMI	GATC	9 cut(s)	75, 685, 919, 991, 1042, 1099, 1182, 1186, 1986
BssNAI	GTATAC	1 cut(s)	1419
BssT1I	CCWWGG	2 cut(s)	1297, 1738
Bst1107I	GTATAC	1 cut(s)	1419
Bst2UI	CCWGG	7 cut(s)	432, 471, 1089, 1159, 1322, 1536, 1938
Bst4CI	ACNGT	9 cut(s)	348, 496, 1053, 1081, 1416, 1927, 2106, 2144, 2167
Bst6I	CTCTTC	8 cut(s)	85, 132, 156, 286, 571, 960, 1554, 1838
BstAFI	CTTAAG	1 cut(s)	533
BstAPI	GCANNNNNTGC	3 cut(s)	1570, 2154, 2396
BstBI	TTCGAA	1 cut(s)	68
BstC8I	GCNNGC	7 cut(s)	353, 530, 717, 1245, 1571, 2155, 2211
BstDEI	CTNAG	4 cut(s)	1403, 1957, 1978, 2361
BstDSI	CCRYGG	1 cut(s)	1738
BstF5I	GGATG	6 cut(s)	564, 940, 1097, 1322, 1540, 2221
BstFNI	CGCG	2 cut(s)	236, 2091
BstHHI	GCGC	5 cut(s)	238, 514, 547, 1737, 1882
BstKTI	GATC	9 cut(s)	78, 688, 922, 994, 1045, 1102, 1185, 1189, 1989
BstMAI	GTCTC	3 cut(s)	1295, 1481, 1827
BstMBI	GATC	9 cut(s)	75, 685, 919, 991, 1042, 1099, 1182, 1186, 1986
BstMCI	CGRYCG	1 cut(s)	1185
BstNI	CCWGG	7 cut(s)	432, 471, 1089, 1159, 1322, 1536, 1938
BstSCI	CCNGG	8 cut(s)	430, 469, 943, 1087, 1157, 1320, 1534, 1936
BstSFI	CTRYAG	4 cut(s)	1018, 1212, 1464, 2158
BstUI	CGCG	2 cut(s)	236, 2091
BstV2I	GAAGAC	3 cut(s)	34, 1269, 2319
BstX2I	RGATCY	3 cut(s)	991, 1042, 1986
BstYI	RGATCY	3 cut(s)	991, 1042, 1986
BstZ17I	GTATAC	1 cut(s)	1419
Bsu15I	ATCGAT	2 cut(s)	1181, 1185
BsuRI	GGCC	6 cut(s)	715, 952, 1202, 1821, 1941, 2130
BsuTUI	ATCGAT	2 cut(s)	1181, 1185
BtgI	CCRYGG	1 cut(s)	1738
BtrI	CACGTC	1 cut(s)	802
BtsCI	GGATG	6 cut(s)	564, 940, 1097, 1322, 1540, 2221
BtsI	GCAGTG	1 cut(s)	2059
BtsIMutI	CAGTG	4 cut(s)	1412, 1923, 2059, 2163
BveI	ACCTGC	1 cut(s)	1736
Cac8I	GCNNGC	7 cut(s)	353, 530, 717, 1245, 1571, 2155, 2211
CaiI	CAGNNNCTG	1 cut(s)	752
CfoI	GCGC	5 cut(s)	238, 514, 547, 1737, 1882
Cfr10I	RCCGGY	1 cut(s)	332
Cfr13I	GGNCC	4 cut(s)	1748, 1912, 2128, 2239
ClaI	ATCGAT	2 cut(s)	1181, 1185
CseI	GACGC	1 cut(s)	1342
Csp6I	GTAC	6 cut(s)	123, 428, 599, 1112, 1154, 1308
CspAI	ACCGGT	1 cut(s)	332
CviAII	CATG	9 cut(s)	199, 509, 924, 1103, 1109, 1519, 1595, 1739, 2003
CviQI	GTAC	6 cut(s)	123, 428, 599, 1112, 1154, 1308
DdeI	CTNAG	4 cut(s)	1403, 1957, 1978, 2361
DpnI	GATC	9 cut(s)	77, 687, 921, 993, 1044, 1101, 1184, 1188, 1988
DpnII	GATC	9 cut(s)	75, 685, 919, 991, 1042, 1099, 1182, 1186, 1986
DraIII	CACNNNGTG	1 cut(s)	2396
EaeI	YGGCCR	1 cut(s)	1200
Eam1104I	CTCTTC	8 cut(s)	85, 132, 156, 286, 571, 960, 1554, 1838
EarI	CTCTTC	8 cut(s)	85, 132, 156, 286, 571, 960, 1554, 1838
EciI	GGCGGA	1 cut(s)	1074
Ecl136II	GAGCTC	2 cut(s)	249, 1975
Eco130I	CCWWGG	2 cut(s)	1297, 1738
Eco147I	AGGCCT	1 cut(s)	1941
Eco24I	GRGCYC	2 cut(s)	251, 1977
Eco47I	GGWCC	3 cut(s)	1748, 1912, 2239
Eco53kI	GAGCTC	2 cut(s)	249, 1975
Eco57I	CTGAAG	2 cut(s)	705, 1800
Eco88I	CYCGRG	1 cut(s)	604
EcoICRI	GAGCTC	2 cut(s)	249, 1975
EcoO109I	RGGNCCY	2 cut(s)	1748, 2239
EcoRII	CCWGG	7 cut(s)	430, 469, 1087, 1157, 1320, 1534, 1936
EcoT14I	CCWWGG	2 cut(s)	1297, 1738
EcoT38I	GRGCYC	2 cut(s)	251, 1977
ErhI	CCWWGG	2 cut(s)	1297, 1738
Esp3I	CGTCTC	1 cut(s)	1827
FaeI	CATG	9 cut(s)	202, 512, 927, 1106, 1112, 1522, 1598, 1742, 2006
FalI	AAGNNNNNCTT	2 cut(s)	890, 922
FaqI	GGGAC	4 cut(s)	1039, 1062, 2309, 2317
FatI	CATG	9 cut(s)	198, 508, 923, 1102, 1108, 1518, 1594, 1738, 2002
FauI	CCCGC	2 cut(s)	206, 679
FblI	GTMKAC	3 cut(s)	255, 1418, 1585
FokI	GGATG	6 cut(s)	551, 927, 1104, 1329, 1527, 2208
FriOI	GRGCYC	2 cut(s)	251, 1977
FspBI	CTAG	4 cut(s)	1194, 1761, 2099, 2274
GlaI	GCGC	5 cut(s)	237, 513, 546, 1736, 1881
GsuI	CTGGAG	3 cut(s)	675, 2020, 2255
HaeIII	GGCC	6 cut(s)	715, 952, 1202, 1821, 1941, 2130
HapII	CCGG	3 cut(s)	333, 945, 1354
HgaI	GACGC	1 cut(s)	1342
HhaI	GCGC	5 cut(s)	238, 514, 547, 1737, 1882
Hin1II	CATG	9 cut(s)	202, 512, 927, 1106, 1112, 1522, 1598, 1742, 2006
Hin6I	GCGC	5 cut(s)	236, 512, 545, 1735, 1880
HinP1I	GCGC	5 cut(s)	236, 512, 545, 1735, 1880
HincII	GTYRAC	2 cut(s)	256, 1372
HindII	GTYRAC	2 cut(s)	256, 1372
HpaII	CCGG	3 cut(s)	333, 945, 1354
HphI	GGTGA	5 cut(s)	322, 1076, 1457, 1988, 2033
Hpy166II	GTNNAC	7 cut(s)	256, 1006, 1310, 1372, 1419, 1586, 2032
Hpy188III	TCNNGA	8 cut(s)	568, 604, 984, 1354, 1480, 1556, 1613, 2234
Hpy8I	GTNNAC	7 cut(s)	256, 1006, 1310, 1372, 1419, 1586, 2032
Hpy99I	CGWCG	5 cut(s)	257, 260, 314, 2303, 2351
HpyAV	CCTTC	5 cut(s)	6, 411, 1263, 2012, 2382
HpyCH4III	ACNGT	9 cut(s)	348, 496, 1053, 1081, 1416, 1927, 2106, 2144, 2167
HpyCH4IV	ACGT	3 cut(s)	801, 1364, 2392
HpyF3I	CTNAG	4 cut(s)	1403, 1957, 1978, 2361
HpySE526I	ACGT	3 cut(s)	801, 1364, 2392
Hsp92II	CATG	9 cut(s)	202, 512, 927, 1106, 1112, 1522, 1598, 1742, 2006
HspAI	GCGC	5 cut(s)	236, 512, 545, 1735, 1880
Kpn2I	TCCGGA	1 cut(s)	1353
KpnI	GGTACC	1 cut(s)	431
Kzo9I	GATC	9 cut(s)	75, 685, 919, 991, 1042, 1099, 1182, 1186, 1986
LguI	GCTCTTC	2 cut(s)	571, 1554
LmnI	GCTCC	6 cut(s)	254, 467, 645, 1301, 1742, 1951
LweI	GCATC	6 cut(s)	315, 449, 862, 1307, 1521, 2002
MaeI	CTAG	4 cut(s)	1194, 1761, 2099, 2274
MaeII	ACGT	3 cut(s)	801, 1364, 2392
MaeIII	GTNAC	6 cut(s)	779, 893, 900, 1456, 1856, 1927
MalI	GATC	9 cut(s)	77, 687, 921, 993, 1044, 1101, 1184, 1188, 1988
MbiI	CCGCTC	1 cut(s)	674
MboI	GATC	9 cut(s)	75, 685, 919, 991, 1042, 1099, 1182, 1186, 1986
MflI	RGATCY	3 cut(s)	991, 1042, 1986
MhlI	GDGCHC	4 cut(s)	251, 353, 1575, 1977
MlsI	TGGCCA	1 cut(s)	1202
MluNI	TGGCCA	1 cut(s)	1202
MlyI	GAGTC	5 cut(s)	270, 308, 401, 1036, 1561
MmeI	TCCRAC	2 cut(s)	1214, 2268
Mox20I	TGGCCA	1 cut(s)	1202
MroI	TCCGGA	1 cut(s)	1353
MscI	TGGCCA	1 cut(s)	1202
MslI	CAYNNNNRTG	4 cut(s)	1107, 1315, 2001, 2168
Msp20I	TGGCCA	1 cut(s)	1202
MspA1I	CMGCKG	5 cut(s)	213, 1402, 1463, 1496, 2405
MspCI	CTTAAG	1 cut(s)	533
MspI	CCGG	3 cut(s)	333, 945, 1354
MspR9I	CCNGG	8 cut(s)	432, 471, 945, 1089, 1159, 1322, 1536, 1938
Mva1269I	GAATGC	1 cut(s)	355
MvaI	CCWGG	7 cut(s)	432, 471, 1089, 1159, 1322, 1536, 1938
MvnI	CGCG	2 cut(s)	236, 2091
NciI	CCSGG	1 cut(s)	945
NcoI	CCATGG	1 cut(s)	1738
NdeII	GATC	9 cut(s)	75, 685, 919, 991, 1042, 1099, 1182, 1186, 1986
NlaIII	CATG	9 cut(s)	202, 512, 927, 1106, 1112, 1522, 1598, 1742, 2006
NlaIV	GGNNCC	4 cut(s)	429, 611, 1988, 2095
NmeAIII	GCCGAG	1 cut(s)	415
NmuCI	GTSAC	2 cut(s)	893, 1456
NspV	TTCGAA	1 cut(s)	68
OliI	CACNNNNGTG	1 cut(s)	2168
PaeR7I	CTCGAG	1 cut(s)	604
PceI	AGGCCT	1 cut(s)	1941
PciSI	GCTCTTC	2 cut(s)	571, 1554
PcsI	WCGNNNNNNNCGW	1 cut(s)	309
PctI	GAATGC	1 cut(s)	355
PfeI	GAWTC	8 cut(s)	407, 419, 474, 749, 827, 1607, 1889, 2416
PfoI	TCCNGGA	1 cut(s)	469
PinAI	ACCGGT	1 cut(s)	332
Ple19I	CGATCG	1 cut(s)	1185
PleI	GAGTC	5 cut(s)	270, 308, 400, 1035, 1560
PpsI	GAGTC	5 cut(s)	270, 308, 400, 1035, 1560
PpuMI	RGGWCCY	2 cut(s)	1748, 2239
Psp124BI	GAGCTC	2 cut(s)	251, 1977
Psp5II	RGGWCCY	2 cut(s)	1748, 2239
Psp6I	CCWGG	7 cut(s)	430, 469, 1087, 1157, 1320, 1534, 1936
PspGI	CCWGG	7 cut(s)	430, 469, 1087, 1157, 1320, 1534, 1936
PspN4I	GGNNCC	4 cut(s)	429, 611, 1988, 2095
PspPI	GGNCC	4 cut(s)	1748, 1912, 2128, 2239
PspPPI	RGGWCCY	2 cut(s)	1748, 2239
PstI	CTGCAG	3 cut(s)	1216, 1468, 2162
PstNI	CAGNNNCTG	1 cut(s)	752
PsuI	RGATCY	3 cut(s)	991, 1042, 1986
PvuI	CGATCG	1 cut(s)	1185
PvuII	CAGCTG	2 cut(s)	1463, 1496
RsaI	GTAC	6 cut(s)	124, 429, 600, 1113, 1155, 1309
RsaNI	GTAC	6 cut(s)	123, 428, 599, 1112, 1154, 1308
RseI	CAYNNNNRTG	4 cut(s)	1107, 1315, 2001, 2168
SacI	GAGCTC	2 cut(s)	251, 1977
SalI	GTCGAC	1 cut(s)	254
SapI	GCTCTTC	2 cut(s)	571, 1554
Sau3AI	GATC	9 cut(s)	75, 685, 919, 991, 1042, 1099, 1182, 1186, 1986
Sau96I	GGNCC	4 cut(s)	1748, 1912, 2128, 2239
ScaI	AGTACT	1 cut(s)	600
SchI	GAGTC	5 cut(s)	270, 308, 401, 1036, 1561
ScrFI	CCNGG	8 cut(s)	432, 471, 945, 1089, 1159, 1322, 1536, 1938
SduI	GDGCHC	4 cut(s)	251, 353, 1575, 1977
SfaNI	GCATC	6 cut(s)	315, 449, 862, 1307, 1521, 2002
SfcI	CTRYAG	4 cut(s)	1018, 1212, 1464, 2158
Sfr274I	CTCGAG	1 cut(s)	604
SfuI	TTCGAA	1 cut(s)	68
SgrDI	CGTCGACG	1 cut(s)	254
SinI	GGWCC	3 cut(s)	1748, 1912, 2239
SlaI	CTCGAG	1 cut(s)	604
SmiMI	CAYNNNNRTG	4 cut(s)	1107, 1315, 2001, 2168
SmlI	CTYRAG	7 cut(s)	360, 533, 604, 784, 982, 1283, 1478
SmoI	CTYRAG	7 cut(s)	360, 533, 604, 784, 982, 1283, 1478
SseBI	AGGCCT	1 cut(s)	1941
SspI	AATATT	1 cut(s)	1488
SspMI	CTAG	4 cut(s)	1194, 1761, 2099, 2274
SstI	GAGCTC	2 cut(s)	251, 1977
StuI	AGGCCT	1 cut(s)	1941
StyD4I	CCNGG	8 cut(s)	430, 469, 943, 1087, 1157, 1320, 1534, 1936
StyI	CCWWGG	2 cut(s)	1297, 1738
TaaI	ACNGT	9 cut(s)	348, 496, 1053, 1081, 1416, 1927, 2106, 2144, 2167
TaiI	ACGT	3 cut(s)	804, 1367, 2395
TaqI	TCGA	9 cut(s)	68, 255, 309, 410, 605, 1181, 1185, 1575, 2283
TatI	WGTACW	2 cut(s)	122, 598
TauI	GCSGC	4 cut(s)	1706, 2402, 2405, 2411
TfiI	GAWTC	8 cut(s)	407, 419, 474, 749, 827, 1607, 1889, 2416
TscAI	CASTG	4 cut(s)	1419, 1930, 2059, 2170
TseFI	GTSAC	2 cut(s)	893, 1456
Tsp45I	GTSAC	2 cut(s)	893, 1456
TspDTI	ATGAA	5 cut(s)	497, 809, 2279, 2325, 2370
TspGWI	ACGGA	2 cut(s)	241, 454
TspRI	CASTG	4 cut(s)	1419, 1930, 2059, 2170
Vha464I	CTTAAG	1 cut(s)	533
VpaK11BI	GGWCC	3 cut(s)	1748, 1912, 2239
XapI	RAATTY	6 cut(s)	706, 832, 1222, 2085, 2198, 2279
XhoI	CTCGAG	1 cut(s)	604
XmiI	GTMKAC	3 cut(s)	255, 1418, 1585
XspI	CTAG	4 cut(s)	1194, 1761, 2099, 2274
ZrmI	AGTACT	1 cut(s)	600

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.