pycom10g23570

Conserved hypothetical protein (Lin0512_fam)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0007945
Physical Location & Seq
Reverse (-)
25669676 .. 25671632
1957 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 750 bp
ATGACAAAAAAAAAAAAAAAAAGTAAAAAACTCAAAAAACTCAAAAAATTAAAATTAAATTTAAAAAAAAAAAAAAAGGCCAGCTTTTACCCATCGAGAGGTGGTGCGAATCATGTCGGGGCAACAAATACAAAATTCAATTTTTTCTGGGCTGAAAAAATGACGGCCTGCTTCTTCCTAAATCTGCCACTCTCTTCTTCTTCATCTACTGCGTTTGCTTCTCACAGTAACTACCAATCACCATCTTCCTTCCCTCCACTTCTCACTCTAATCTCAACCCAATTCTCCCATCTTCGAACCCCTAGATTCTCTCGCCAAATCAGAGCCGCATCAGCTGCCATGGAAGCCACCCAGCAGGGCGAGACCACCGCCTCGGCGTCGCCCATGAAGCTCTTGTTCGTGGAGATGGGCGTCGGCTACGACCAACACGGGCAGGACGTTACGTCTGCGGCAATGCGGGCTTGCAGAGATGCCATCACTTCCAATTCAATCCCTGCGTTTCGAAGAGGGTCAATTCCCGGTGTGACATTTGAGCAGATGAAGCTAGAGATCAAGCTGGGGGTGCCTCGTGTACTCCAACCATCGCTGGATGTCGAGAAGGTCAAATCAGTCTTTCCTTATGGAGAAATTGTGAAAGTTGAAGTTGTGGATGGTGGACTGATATGCTCAAGTGGCGTGTTGGTGGAAGAAATGGGAGATAAGACTGACGACTGTTACATAGTGAATGCAGCAGTTTACATTGGTTACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lin0512_fam

Protein Analysis

250

Amino Acids

27.34

Weight (kDa)

9.79

Isoelectric Point (pI)

32.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lin0512_fam PF09585 132 - 246 1.3e-37 Conserved hypothetical protein (Lin0512_fam)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 562
AciI CCGC 4 cut(s) 327, 369, 449, 457
AcsI RAATTY 2 cut(s) 58, 134
AcyI GRCGYC 2 cut(s) 377, 411
AfaI GTAC 1 cut(s) 573
AfiI CCNNNNNNNGG 1 cut(s) 98
AgsI TTSAA 3 cut(s) 139, 489, 641
AhdI GACNNNNNGTC 1 cut(s) 442
AluBI AGCT 5 cut(s) 84, 335, 391, 544, 556
AluI AGCT 5 cut(s) 84, 335, 391, 544, 556
Alw26I GTCTC 1 cut(s) 356
AoxI GGCC 2 cut(s) 78, 165
ApeKI GCWGC 2 cut(s) 335, 728
ApoI RAATTY 2 cut(s) 58, 134
AsuC2I CCSGG 1 cut(s) 519
AsuHPI GGTGA 1 cut(s) 231
AsuII TTCGAA 2 cut(s) 295, 502
BanI GGYRCC 1 cut(s) 562
BauI CACGAG 1 cut(s) 567
BbvI GCAGC 2 cut(s) 322, 740
BccI CCATC 7 cut(s) 100, 250, 297, 400, 482, 589, 644
BceAI ACGGC 1 cut(s) 180
BcnI CCSGG 1 cut(s) 519
BcoDI GTCTC 1 cut(s) 356
BfaI CTAG 3 cut(s) 303, 545, 748
BisI GCNGC 4 cut(s) 327, 336, 450, 729
BlsI GCNGC 4 cut(s) 328, 337, 451, 730
Bme1390I CCNGG 1 cut(s) 519
BmeRI GACNNNNNGTC 1 cut(s) 442
BmiI GGNNCC 1 cut(s) 564
BmrFI CCNGG 1 cut(s) 519
BmsI GCATC 2 cut(s) 338, 460
Bpu14I TTCGAA 2 cut(s) 295, 502
BpuEI CTTGAG 1 cut(s) 652
BpuMI CCSGG 1 cut(s) 519
BsaHI GRCGYC 2 cut(s) 377, 411
BsaI GGTCTC 1 cut(s) 356
BsaJI CCNNGG 2 cut(s) 339, 372
BsaXI ACNNNNNCTCC 2 cut(s) 615, 645
Bsc4I CCNNNNNNNGG 1 cut(s) 98
Bse3DI GCAATG 1 cut(s) 459
BseDI CCNNGG 2 cut(s) 339, 372
BseGI GGATG 2 cut(s) 595, 655
BseLI CCNNNNNNNGG 1 cut(s) 98
BseMI GCAATG 1 cut(s) 459
BseXI GCAGC 2 cut(s) 322, 740
BseYI CCCAGC 2 cut(s) 351, 556
BshFI GGCC 2 cut(s) 80, 167
BshNI GGYRCC 1 cut(s) 562
BsiSI CCGG 1 cut(s) 519
BslI CCNNNNNNNGG 1 cut(s) 98
BsmAI GTCTC 1 cut(s) 356
BsmI GAATGC 1 cut(s) 730
BsnI GGCC 2 cut(s) 80, 167
Bso31I GGTCTC 1 cut(s) 356
Bsp119I TTCGAA 2 cut(s) 295, 502
Bsp143I GATC 1 cut(s) 549
Bsp19I CCATGG 1 cut(s) 339
BspACI CCGC 4 cut(s) 327, 369, 449, 457
BspANI GGCC 2 cut(s) 80, 167
BspLI GGNNCC 1 cut(s) 564
BspT104I TTCGAA 2 cut(s) 295, 502
BspT107I GGYRCC 1 cut(s) 562
BspTNI GGTCTC 1 cut(s) 356
BsrDI GCAATG 1 cut(s) 459
BssECI CCNNGG 2 cut(s) 339, 372
BssMI GATC 1 cut(s) 549
BssNI GRCGYC 2 cut(s) 377, 411
BssSI CACGAG 1 cut(s) 567
BssT1I CCWWGG 1 cut(s) 339
Bst2BI CACGAG 1 cut(s) 567
Bst4CI ACNGT 2 cut(s) 227, 713
Bst6I CTCTTC 2 cut(s) 199, 499
BstACI GRCGYC 2 cut(s) 377, 411
BstAPI GCANNNNNTGC 1 cut(s) 335
BstBI TTCGAA 2 cut(s) 295, 502
BstC8I GCNNGC 4 cut(s) 82, 169, 459, 463
BstDSI CCRYGG 1 cut(s) 339
BstF5I GGATG 2 cut(s) 595, 655
BstKTI GATC 1 cut(s) 552
BstMAI GTCTC 1 cut(s) 356
BstMBI GATC 1 cut(s) 549
BstMWI GCNNNNNNNGC 8 cut(s) 332, 335, 344, 388, 458, 541, 562, 672
BstSCI CCNGG 1 cut(s) 517
BstV1I GCAGC 2 cut(s) 322, 740
BsuRI GGCC 2 cut(s) 80, 167
BtgI CCRYGG 1 cut(s) 339
BtgZI GCGATG 1 cut(s) 567
BtsCI GGATG 2 cut(s) 595, 655
Cac8I GCNNGC 4 cut(s) 82, 169, 459, 463
CseI GACGC 2 cut(s) 366, 400
Csp6I GTAC 1 cut(s) 572
CviAII CATG 3 cut(s) 113, 340, 385
CviQI GTAC 1 cut(s) 572
DpnI GATC 1 cut(s) 551
DpnII GATC 1 cut(s) 549
DraI TTTAAA 1 cut(s) 63
DriI GACNNNNNGTC 1 cut(s) 442
Eam1104I CTCTTC 2 cut(s) 199, 499
Eam1105I GACNNNNNGTC 1 cut(s) 442
EarI CTCTTC 2 cut(s) 199, 499
Eco130I CCWWGG 1 cut(s) 339
Eco31I GGTCTC 1 cut(s) 356
EcoT14I CCWWGG 1 cut(s) 339
ErhI CCWWGG 1 cut(s) 339
FaeI CATG 3 cut(s) 116, 343, 388
FaiI YATR 6 cut(s) 114, 341, 386, 621, 664, 719
FalI AAGNNNNNCTT 2 cut(s) 68, 100
FatI CATG 3 cut(s) 112, 339, 384
FauI CCCGC 1 cut(s) 450
Fnu4HI GCNGC 4 cut(s) 327, 336, 450, 729
FokI GGATG 2 cut(s) 602, 662
Fsp4HI GCNGC 4 cut(s) 327, 336, 450, 729
FspBI CTAG 3 cut(s) 303, 545, 748
GluI GCNGC 4 cut(s) 327, 336, 450, 729
GsaI CCCAGC 2 cut(s) 355, 560
HaeIII GGCC 2 cut(s) 80, 167
HapII CCGG 1 cut(s) 519
HgaI GACGC 2 cut(s) 366, 400
Hin1I GRCGYC 2 cut(s) 377, 411
Hin1II CATG 3 cut(s) 116, 343, 388
HinfI GANTC 2 cut(s) 109, 306
HpaII CCGG 1 cut(s) 519
HphI GGTGA 1 cut(s) 231
Hpy166II GTNNAC 3 cut(s) 572, 656, 736
Hpy188I TCNGA 1 cut(s) 323
Hpy188III TCNNGA 2 cut(s) 96, 595
Hpy8I GTNNAC 3 cut(s) 572, 656, 736
Hpy99I CGWCG 2 cut(s) 382, 416
HpyAV CCTTC 2 cut(s) 259, 592
HpyCH4III ACNGT 2 cut(s) 227, 713
HpyCH4IV ACGT 2 cut(s) 438, 443
HpyCH4V TGCA 2 cut(s) 465, 728
HpyF10VI GCNNNNNNNGC 8 cut(s) 332, 335, 344, 388, 458, 541, 562, 672
HpySE526I ACGT 2 cut(s) 438, 443
Hsp92I GRCGYC 2 cut(s) 377, 411
Hsp92II CATG 3 cut(s) 116, 343, 388
Kzo9I GATC 1 cut(s) 549
Lsp1109I GCAGC 2 cut(s) 322, 740
LweI GCATC 2 cut(s) 338, 460
MaeI CTAG 3 cut(s) 303, 545, 748
MaeII ACGT 2 cut(s) 438, 443
MaeIII GTNAC 5 cut(s) 227, 439, 523, 713, 743
MalI GATC 1 cut(s) 551
MboI GATC 1 cut(s) 549
MboII GAAGA 8 cut(s) 166, 186, 189, 192, 237, 284, 516, 698
MluCI AATT 9 cut(s) 47, 53, 58, 134, 139, 281, 484, 513, 627
MmeI TCCRAC 1 cut(s) 601
MnlI CCTC 5 cut(s) 92, 264, 382, 500, 576
MseI TTAA 3 cut(s) 50, 56, 62
MspA1I CMGCKG 1 cut(s) 335
MspI CCGG 1 cut(s) 519
MspR9I CCNGG 1 cut(s) 519
Mva1269I GAATGC 1 cut(s) 730
MwoI GCNNNNNNNGC 8 cut(s) 332, 335, 344, 388, 458, 541, 562, 672
NciI CCSGG 1 cut(s) 519
NcoI CCATGG 1 cut(s) 339
NdeII GATC 1 cut(s) 549
NlaIII CATG 3 cut(s) 116, 343, 388
NlaIV GGNNCC 1 cut(s) 564
NmeAIII GCCGAG 1 cut(s) 353
NmuCI GTSAC 1 cut(s) 523
NspV TTCGAA 2 cut(s) 295, 502
PcsI WCGNNNNNNNCGW 1 cut(s) 435
PctI GAATGC 1 cut(s) 730
PfeI GAWTC 2 cut(s) 109, 306
PkrI GCNGC 4 cut(s) 328, 337, 451, 730
PspFI CCCAGC 2 cut(s) 351, 556
PspN4I GGNNCC 1 cut(s) 564
PvuII CAGCTG 1 cut(s) 335
RsaI GTAC 1 cut(s) 573
RsaNI GTAC 1 cut(s) 572
SaqAI TTAA 3 cut(s) 50, 56, 62
SatI GCNGC 4 cut(s) 327, 336, 450, 729
Sau3AI GATC 1 cut(s) 549
ScrFI CCNGG 1 cut(s) 519
SetI ASST 9 cut(s) 86, 103, 337, 393, 441, 446, 546, 558, 603
SfaNI GCATC 2 cut(s) 338, 460
SfuI TTCGAA 2 cut(s) 295, 502
SmlI CTYRAG 1 cut(s) 667
SmoI CTYRAG 1 cut(s) 667
Sse9I AATT 9 cut(s) 47, 53, 58, 134, 139, 281, 484, 513, 627
SsiI CCGC 4 cut(s) 327, 369, 449, 457
SspMI CTAG 3 cut(s) 303, 545, 748
StyD4I CCNGG 1 cut(s) 517
StyI CCWWGG 1 cut(s) 339
TaaI ACNGT 2 cut(s) 227, 713
TaiI ACGT 2 cut(s) 441, 446
TaqI TCGA 4 cut(s) 95, 295, 502, 594
TasI AATT 9 cut(s) 47, 53, 58, 134, 139, 281, 484, 513, 627
TatI WGTACW 1 cut(s) 571
TauI GCSGC 2 cut(s) 329, 452
TfiI GAWTC 2 cut(s) 109, 306
Tru1I TTAA 3 cut(s) 50, 56, 62
Tru9I TTAA 3 cut(s) 50, 56, 62
TseFI GTSAC 1 cut(s) 523
TseI GCWGC 2 cut(s) 335, 728
Tsp45I GTSAC 1 cut(s) 523
TspDTI ATGAA 3 cut(s) 192, 401, 554
XapI RAATTY 2 cut(s) 58, 134
XspI CTAG 3 cut(s) 303, 545, 748
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.