pycom10g24740

Heat Stress Transcription Factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0013636
Physical Location & Seq
Forward (+)
26527751 .. 26529740
1990 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 570 bp
ATGGCTACAAAATCTTCCCGGACAAGAAGCACCGCTCCGTTTCTGTCGAAGACGTTCGATTTGCTAGAAGAAAATGACGGAGGAGATGGTGGTGGGAAGAGAAATATTGTGTCATGGAATGTAGAGGGCACTGGATTTATAGTATGGTCTCCTGATGATTTCTCAGAGCTCTTGTTGCCTAAATATTTCAAGCACAGCAATTTCTCCAGCTTCATTCGCCAGCTTAATACCTATGGATTCAAAAAAACATCACGAAAAGAGTGGGAATTTAAGCATGAGAAATTCCAGAAGGGCTGCAGGGAGATGCTAGTGGAGATCACCAGGAAGAAATGCGAACCAAGTGCTTTTCCAGTGTATCTAAAGGCTTCAGAAGAGAGTGGGAGCACAAGCACTGCAGTGGCAGCAGCAGAAGAAAACAACTGCCTCCTATTAATGGAGGAGAATAAGAACCTGAGAAAGCAGAAGTTGGAGCTGCAGGTGCAAATAGCTCAGTTCAAAACACTTGAAAGTAAGCTGTTGGACTGCGTTGCTCAGTGCATGGACGACCATCAGAACAATGTACGATGCTGA

Protein Analysis

190

Amino Acids

21.61

Weight (kDa)

8.17

Isoelectric Point (pI)

43.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSF_DNA-bind PF00447 14 - 109 2.9e-26 HSF-type DNA-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 466
Acc36I ACCTGC 1 cut(s) 466
AccBSI CCGCTC 1 cut(s) 35
AciI CCGC 1 cut(s) 33
AcsI RAATTY 2 cut(s) 266, 281
AcuI CTGAAG 1 cut(s) 351
AfaI GTAC 1 cut(s) 561
AfiI CCNNNNNNNGG 1 cut(s) 433
AgsI TTSAA 4 cut(s) 190, 241, 496, 506
AjnI CCWGG 1 cut(s) 320
AleI CACNNNNGTG 1 cut(s) 395
AluBI AGCT 6 cut(s) 169, 210, 223, 472, 488, 514
AluI AGCT 6 cut(s) 169, 210, 223, 472, 488, 514
Alw21I GWGCWC 2 cut(s) 171, 386
Alw26I GTCTC 1 cut(s) 153
ApeKI GCWGC 4 cut(s) 294, 401, 404, 472
ApoI RAATTY 2 cut(s) 266, 281
ArsI GACNNNNNNTTYG 2 cut(s) 43, 75
AseI ATTAAT 1 cut(s) 431
Asp700I GAANNNNTTC 1 cut(s) 53
AsuC2I CCSGG 1 cut(s) 19
AsuHPI GGTGA 1 cut(s) 310
BaeGI GKGCMC 1 cut(s) 131
BanII GRGCYC 1 cut(s) 171
BbsI GAAGAC 1 cut(s) 56
Bbv12I GWGCWC 2 cut(s) 171, 386
BbvI GCAGC 4 cut(s) 281, 413, 416, 459
BccI CCATC 2 cut(s) 80, 555
BcgI CGANNNNNNTGC 2 cut(s) 323, 357
BciT130I CCWGG 1 cut(s) 322
BcnI CCSGG 1 cut(s) 19
BcoDI GTCTC 1 cut(s) 153
BfaI CTAG 2 cut(s) 65, 308
BfmI CTRYAG 3 cut(s) 295, 393, 473
BfuAI ACCTGC 1 cut(s) 466
BisI GCNGC 4 cut(s) 295, 402, 405, 473
BlsI GCNGC 4 cut(s) 296, 403, 406, 474
Bme1390I CCNGG 2 cut(s) 19, 322
BmrFI CCNGG 2 cut(s) 19, 322
BmsI GCATC 2 cut(s) 294, 554
BpiI GAAGAC 1 cut(s) 56
BpmI CTGGAG 1 cut(s) 190
BpuMI CCSGG 1 cut(s) 19
BsaI GGTCTC 1 cut(s) 153
Bsc4I CCNNNNNNNGG 1 cut(s) 433
Bse1I ACTGG 2 cut(s) 136, 350
BseBI CCWGG 1 cut(s) 322
BseLI CCNNNNNNNGG 1 cut(s) 433
BseMII CTCAG 4 cut(s) 177, 443, 503, 545
BseNI ACTGG 2 cut(s) 136, 350
BseRI GAGGAG 2 cut(s) 96, 452
BseSI GKGCMC 1 cut(s) 131
BseXI GCAGC 4 cut(s) 281, 413, 416, 459
BsiHKAI GWGCWC 2 cut(s) 171, 386
BsiSI CCGG 1 cut(s) 19
BslI CCNNNNNNNGG 1 cut(s) 433
BsmAI GTCTC 1 cut(s) 153
Bso31I GGTCTC 1 cut(s) 153
Bsp1286I GDGCHC 3 cut(s) 131, 171, 386
Bsp143I GATC 1 cut(s) 315
BspACI CCGC 1 cut(s) 33
BspCNI CTCAG 4 cut(s) 176, 444, 502, 544
BspMAI CTGCAG 3 cut(s) 299, 397, 477
BspMI ACCTGC 1 cut(s) 466
BspTNI GGTCTC 1 cut(s) 153
BsrBI CCGCTC 1 cut(s) 35
BsrI ACTGG 2 cut(s) 136, 350
BssMI GATC 1 cut(s) 315
Bst2UI CCWGG 1 cut(s) 322
Bst6I CTCTTC 2 cut(s) 92, 366
BstC8I GCNNGC 1 cut(s) 221
BstDEI CTNAG 4 cut(s) 163, 452, 489, 531
BstKTI GATC 1 cut(s) 318
BstMAI GTCTC 1 cut(s) 153
BstMBI GATC 1 cut(s) 315
BstMWI GCNNNNNNNGC 4 cut(s) 175, 216, 401, 478
BstNI CCWGG 1 cut(s) 322
BstSCI CCNGG 2 cut(s) 17, 320
BstSFI CTRYAG 3 cut(s) 295, 393, 473
BstSLI GKGCMC 1 cut(s) 131
BstV1I GCAGC 4 cut(s) 281, 413, 416, 459
BstV2I GAAGAC 1 cut(s) 56
BtsI GCAGTG 2 cut(s) 390, 402
BtsIMutI CAGTG 5 cut(s) 129, 357, 390, 402, 539
BveI ACCTGC 1 cut(s) 466
Cac8I GCNNGC 1 cut(s) 221
Csp6I GTAC 1 cut(s) 560
CviAII CATG 3 cut(s) 114, 275, 538
CviJI RGCY 9 cut(s) 5, 169, 210, 223, 294, 365, 472, 488, 514
CviKI_1 RGCY 9 cut(s) 5, 169, 210, 223, 294, 365, 472, 488, 514
CviQI GTAC 1 cut(s) 560
DdeI CTNAG 4 cut(s) 163, 452, 489, 531
DpnI GATC 1 cut(s) 317
DpnII GATC 1 cut(s) 315
Eam1104I CTCTTC 2 cut(s) 92, 366
EarI CTCTTC 2 cut(s) 92, 366
Ecl136II GAGCTC 1 cut(s) 169
Eco24I GRGCYC 1 cut(s) 171
Eco31I GGTCTC 1 cut(s) 153
Eco53kI GAGCTC 1 cut(s) 169
Eco57I CTGAAG 1 cut(s) 351
EcoICRI GAGCTC 1 cut(s) 169
EcoRII CCWGG 1 cut(s) 320
EcoT38I GRGCYC 1 cut(s) 171
FaeI CATG 3 cut(s) 117, 278, 541
FaiI YATR 6 cut(s) 115, 140, 145, 234, 276, 539
FatI CATG 3 cut(s) 113, 274, 537
Fnu4HI GCNGC 4 cut(s) 295, 402, 405, 473
FriOI GRGCYC 1 cut(s) 171
Fsp4HI GCNGC 4 cut(s) 295, 402, 405, 473
FspBI CTAG 2 cut(s) 65, 308
GluI GCNGC 4 cut(s) 295, 402, 405, 473
GsuI CTGGAG 1 cut(s) 190
HapII CCGG 1 cut(s) 19
Hin1II CATG 3 cut(s) 117, 278, 541
HinfI GANTC 1 cut(s) 237
HpaII CCGG 1 cut(s) 19
HphI GGTGA 1 cut(s) 310
Hpy188I TCNGA 3 cut(s) 166, 370, 552
Hpy188III TCNNGA 3 cut(s) 152, 252, 286
HpyAV CCTTC 1 cut(s) 283
HpyCH4IV ACGT 1 cut(s) 53
HpyCH4V TGCA 5 cut(s) 297, 395, 475, 481, 537
HpyF10VI GCNNNNNNNGC 4 cut(s) 175, 216, 401, 478
HpyF3I CTNAG 4 cut(s) 163, 452, 489, 531
HpySE526I ACGT 1 cut(s) 53
Hsp92II CATG 3 cut(s) 117, 278, 541
Kzo9I GATC 1 cut(s) 315
LmnI GCTCC 3 cut(s) 40, 381, 469
Lsp1109I GCAGC 4 cut(s) 281, 413, 416, 459
LweI GCATC 2 cut(s) 294, 554
MaeI CTAG 2 cut(s) 65, 308
MaeII ACGT 1 cut(s) 53
MalI GATC 1 cut(s) 317
MbiI CCGCTC 1 cut(s) 35
MboI GATC 1 cut(s) 315
MboII GAAGA 7 cut(s) 6, 61, 80, 109, 337, 383, 422
MhlI GDGCHC 3 cut(s) 131, 171, 386
MluCI AATT 3 cut(s) 199, 266, 281
MmeI TCCRAC 2 cut(s) 447, 498
MnlI CCTC 4 cut(s) 74, 118, 430, 434
MroXI GAANNNNTTC 1 cut(s) 53
MseI TTAA 3 cut(s) 225, 270, 431
MslI CAYNNNNRTG 1 cut(s) 395
MspI CCGG 1 cut(s) 19
MspR9I CCNGG 2 cut(s) 19, 322
MvaI CCWGG 1 cut(s) 322
MwoI GCNNNNNNNGC 4 cut(s) 175, 216, 401, 478
NciI CCSGG 1 cut(s) 19
NdeII GATC 1 cut(s) 315
NlaIII CATG 3 cut(s) 117, 278, 541
OliI CACNNNNGTG 1 cut(s) 395
PaqCI CACCTGC 1 cut(s) 466
PdmI GAANNNNTTC 1 cut(s) 53
PfeI GAWTC 1 cut(s) 237
PfoI TCCNGGA 1 cut(s) 17
PkrI GCNGC 4 cut(s) 296, 403, 406, 474
PshBI ATTAAT 1 cut(s) 431
Psp124BI GAGCTC 1 cut(s) 171
Psp6I CCWGG 1 cut(s) 320
PspGI CCWGG 1 cut(s) 320
PstI CTGCAG 3 cut(s) 299, 397, 477
RsaI GTAC 1 cut(s) 561
RsaNI GTAC 1 cut(s) 560
RseI CAYNNNNRTG 1 cut(s) 395
SacI GAGCTC 1 cut(s) 171
SaqAI TTAA 3 cut(s) 225, 270, 431
SatI GCNGC 4 cut(s) 295, 402, 405, 473
Sau3AI GATC 1 cut(s) 315
ScrFI CCNGG 2 cut(s) 19, 322
SduI GDGCHC 3 cut(s) 131, 171, 386
SfaNI GCATC 2 cut(s) 294, 554
SfcI CTRYAG 3 cut(s) 295, 393, 473
SmiMI CAYNNNNRTG 1 cut(s) 395
Sse9I AATT 3 cut(s) 199, 266, 281
SsiI CCGC 1 cut(s) 33
SspI AATATT 2 cut(s) 106, 185
SspMI CTAG 2 cut(s) 65, 308
SstI GAGCTC 1 cut(s) 171
StyD4I CCNGG 2 cut(s) 17, 320
TaiI ACGT 1 cut(s) 56
TaqI TCGA 2 cut(s) 47, 57
TasI AATT 3 cut(s) 199, 266, 281
TfiI GAWTC 1 cut(s) 237
Tru1I TTAA 3 cut(s) 225, 270, 431
Tru9I TTAA 3 cut(s) 225, 270, 431
TscAI CASTG 5 cut(s) 136, 357, 397, 402, 539
TseI GCWGC 4 cut(s) 294, 401, 404, 472
TspDTI ATGAA 1 cut(s) 202
TspGWI ACGGA 2 cut(s) 27, 93
TspRI CASTG 5 cut(s) 136, 357, 397, 402, 539
VspI ATTAAT 1 cut(s) 431
XapI RAATTY 2 cut(s) 266, 281
XmnI GAANNNNTTC 1 cut(s) 53
XspI CTAG 2 cut(s) 65, 308
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.