RchiOBHm_Chr5g0008251

Heat Stress Transcription Factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0013636
Physical Location & Seq
Reverse (-)
5263280 .. 5263666
387 bp
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UTR
Exon/CDS
Intron
PRQ28923

Sequence Viewer

Length: 387 bp
ATGTGTATGGATCAACATATCTCGGAGGTGAACCATGGCACAAGTCAGAGTTCGAGGACGAGAAGCCCTGCCCCGTTTTTGTCGAAAACGTACGATTTGCTAGAGGAAGAAGAAGAGGAAGACAAAGATGGCAGCTGGAAGAGATCAATTGTGTCATGGAATGGAGAGGGAAATGGGTTTATAGTATGGTCTCCTGCTGAGTTCTCAGAGCTCTTGTTGCCTAAATACTTCAAGCACAATAACTTCTCCAGCTTTATTCGCCAGCTTAATACCTATGTGAGTAGTCTAAATTCCAGTCTTGCTAGCTCTATCTTCATTTATATACAGAATGAAATATCATACAGATCCATCAATGGCTTATATATAACTACTATTTCAATGATGTGA

Protein Analysis

128

Amino Acids

14.71

Weight (kDa)

5.1

Isoelectric Point (pI)

69.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSF_DNA-bind PF00447 26 - 92 6.5e-17 HSF-type DNA-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 2 cut(s) 18, 339
AcsI RAATTY 1 cut(s) 289
AfaI GTAC 1 cut(s) 92
AgsI TTSAA 2 cut(s) 232, 378
AluBI AGCT 5 cut(s) 135, 211, 252, 265, 306
AluI AGCT 5 cut(s) 135, 211, 252, 265, 306
Alw21I GWGCWC 1 cut(s) 213
Alw26I GTCTC 1 cut(s) 195
AlwI GGATC 2 cut(s) 18, 339
ApeKI GCWGC 1 cut(s) 132
ApoI RAATTY 1 cut(s) 289
AsuHPI GGTGA 1 cut(s) 40
AsuNHI GCTAGC 1 cut(s) 302
BanII GRGCYC 1 cut(s) 213
BbsI GAAGAC 1 cut(s) 126
Bbv12I GWGCWC 1 cut(s) 213
BbvI GCAGC 1 cut(s) 144
BccI CCATC 2 cut(s) 122, 356
BcoDI GTCTC 1 cut(s) 195
BfaI CTAG 2 cut(s) 101, 303
BisI GCNGC 1 cut(s) 133
BlsI GCNGC 1 cut(s) 134
BmtI GCTAGC 1 cut(s) 306
BpiI GAAGAC 1 cut(s) 126
BpmI CTGGAG 1 cut(s) 232
BsaI GGTCTC 1 cut(s) 195
BsaJI CCNNGG 1 cut(s) 34
Bse1I ACTGG 1 cut(s) 294
BseDI CCNNGG 1 cut(s) 34
BseMII CTCAG 2 cut(s) 189, 219
BseNI ACTGG 1 cut(s) 294
BseXI GCAGC 1 cut(s) 144
BsiHKAI GWGCWC 1 cut(s) 213
BsiWI CGTACG 1 cut(s) 90
BsmAI GTCTC 1 cut(s) 195
Bso31I GGTCTC 1 cut(s) 195
Bsp1286I GDGCHC 1 cut(s) 213
Bsp143I GATC 3 cut(s) 10, 143, 344
Bsp19I CCATGG 1 cut(s) 34
BspCNI CTCAG 2 cut(s) 190, 218
BspOI GCTAGC 1 cut(s) 306
BspPI GGATC 2 cut(s) 18, 339
BspTNI GGTCTC 1 cut(s) 195
BsrI ACTGG 1 cut(s) 294
BssECI CCNNGG 1 cut(s) 34
BssMI GATC 3 cut(s) 10, 143, 344
BssT1I CCWWGG 1 cut(s) 34
Bst6I CTCTTC 2 cut(s) 108, 134
BstC8I GCNNGC 2 cut(s) 263, 304
BstDEI CTNAG 2 cut(s) 198, 205
BstDSI CCRYGG 1 cut(s) 34
BstKTI GATC 3 cut(s) 13, 146, 347
BstMAI GTCTC 1 cut(s) 195
BstMBI GATC 3 cut(s) 10, 143, 344
BstMWI GCNNNNNNNGC 2 cut(s) 217, 258
BstV1I GCAGC 1 cut(s) 144
BstV2I GAAGAC 1 cut(s) 126
BstX2I RGATCY 1 cut(s) 344
BstYI RGATCY 1 cut(s) 344
BtgI CCRYGG 1 cut(s) 34
Cac8I GCNNGC 2 cut(s) 263, 304
Csp6I GTAC 1 cut(s) 91
CviAII CATG 2 cut(s) 35, 156
CviJI RGCY 7 cut(s) 66, 135, 211, 252, 265, 306, 357
CviKI_1 RGCY 7 cut(s) 66, 135, 211, 252, 265, 306, 357
CviQI GTAC 1 cut(s) 91
DdeI CTNAG 2 cut(s) 198, 205
DpnI GATC 3 cut(s) 12, 145, 346
DpnII GATC 3 cut(s) 10, 143, 344
Eam1104I CTCTTC 2 cut(s) 108, 134
EarI CTCTTC 2 cut(s) 108, 134
Ecl136II GAGCTC 1 cut(s) 211
Eco130I CCWWGG 1 cut(s) 34
Eco24I GRGCYC 1 cut(s) 213
Eco31I GGTCTC 1 cut(s) 195
Eco53kI GAGCTC 1 cut(s) 211
EcoICRI GAGCTC 1 cut(s) 211
EcoT14I CCWWGG 1 cut(s) 34
EcoT38I GRGCYC 1 cut(s) 213
ErhI CCWWGG 1 cut(s) 34
FaeI CATG 2 cut(s) 38, 159
FatI CATG 2 cut(s) 34, 155
Fnu4HI GCNGC 1 cut(s) 133
FriOI GRGCYC 1 cut(s) 213
Fsp4HI GCNGC 1 cut(s) 133
FspBI CTAG 2 cut(s) 101, 303
GluI GCNGC 1 cut(s) 133
GsuI CTGGAG 1 cut(s) 232
Hin1II CATG 2 cut(s) 38, 159
HphI GGTGA 1 cut(s) 40
Hpy166II GTNNAC 1 cut(s) 31
Hpy188I TCNGA 3 cut(s) 25, 48, 208
Hpy8I GTNNAC 1 cut(s) 31
HpyCH4IV ACGT 1 cut(s) 89
HpyF10VI GCNNNNNNNGC 2 cut(s) 217, 258
HpyF3I CTNAG 2 cut(s) 198, 205
HpySE526I ACGT 1 cut(s) 89
Hsp92II CATG 2 cut(s) 38, 159
Kzo9I GATC 3 cut(s) 10, 143, 344
LpnPI CCDG 6 cut(s) 81, 121, 207, 262, 275, 307
Lsp1109I GCAGC 1 cut(s) 144
MaeI CTAG 2 cut(s) 101, 303
MaeII ACGT 1 cut(s) 89
MalI GATC 3 cut(s) 12, 145, 346
MboI GATC 3 cut(s) 10, 143, 344
MboII GAAGA 6 cut(s) 119, 122, 125, 131, 151, 304
MfeI CAATTG 1 cut(s) 147
MflI RGATCY 1 cut(s) 344
MhlI GDGCHC 1 cut(s) 213
MluCI AATT 2 cut(s) 147, 289
MnlI CCTC 5 cut(s) 19, 48, 97, 109, 160
MseI TTAA 1 cut(s) 267
MspA1I CMGCKG 1 cut(s) 135
MunI CAATTG 1 cut(s) 147
MwoI GCNNNNNNNGC 2 cut(s) 217, 258
NcoI CCATGG 1 cut(s) 34
NdeII GATC 3 cut(s) 10, 143, 344
NheI GCTAGC 1 cut(s) 302
NlaIII CATG 2 cut(s) 38, 159
Pfl23II CGTACG 1 cut(s) 90
PkrI GCNGC 1 cut(s) 134
Psp124BI GAGCTC 1 cut(s) 213
PspLI CGTACG 1 cut(s) 90
PsuI RGATCY 1 cut(s) 344
PvuII CAGCTG 1 cut(s) 135
RsaI GTAC 1 cut(s) 92
RsaNI GTAC 1 cut(s) 91
SacI GAGCTC 1 cut(s) 213
SaqAI TTAA 1 cut(s) 267
SatI GCNGC 1 cut(s) 133
Sau3AI GATC 3 cut(s) 10, 143, 344
SduI GDGCHC 1 cut(s) 213
SetI ASST 8 cut(s) 30, 92, 137, 213, 254, 267, 275, 308
Sse9I AATT 2 cut(s) 147, 289
SspMI CTAG 2 cut(s) 101, 303
SstI GAGCTC 1 cut(s) 213
StyI CCWWGG 1 cut(s) 34
TaiI ACGT 1 cut(s) 92
TaqI TCGA 2 cut(s) 53, 83
TasI AATT 2 cut(s) 147, 289
Tru1I TTAA 1 cut(s) 267
Tru9I TTAA 1 cut(s) 267
TseI GCWGC 1 cut(s) 132
TspDTI ATGAA 2 cut(s) 304, 345
XapI RAATTY 1 cut(s) 289
XspI CTAG 2 cut(s) 101, 303
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.