RLG00000031554

Heat Stress Transcription Factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0013636
Physical Location & Seq
Reverse (-)
6100685 .. 6102386
1702 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000031554

Sequence Viewer

Length: 618 bp
ATGTGTATGGATCAACATATCTCGGAGGTGAACCATGGCACAAGTCAGAGTTCGAGGACGAGAAGCCCTGCCCCGTTTTTGTCGAAAACGTATGATTTGCTAGAGGAAGAAGAAGAGGAAGACGAAGATGGCAGCGGAAAGAGATCAATTGTGTCATGGAATGGAGAGGGAAATGGGTTTATAGTATGGTCTCCTGCTGAGTTCTCAGAGCTCTTGTTGCCTAAATACTTCAAGCACAATAACTTCTCCAGCTTTATTCGCCAGCTTAATACCTATGGATTCAAGAAAACATCACCAAAACGATGGGAATTCAAACACGAGAAGTTCCAGAAGGGATGCCGGCATCTGCTACTGGAGATCAATCGGAAGAAATGCGAACCAAGCGCTTTTCCGGTGTATCTAAAGGCTTCGGAAGAGAGCACTAGTGCTACTGGCCTTAATCGAAGCATGAACAATGCAGCAGAACAAGAGAATCAGTCTTTGCTGCTAATGGAGGAGAACAAGAACCTAAGGAAGCAGAAAATGGAGCTTCAGATGCAACTTTCTCAGTTCAAAGCACTAGAAAGTAAGCTGTTGGATTGTGTGGGTCAGTATATGCAAGACCATCACAATCGATGA

Protein Analysis

206

Amino Acids

23.85

Weight (kDa)

6.67

Isoelectric Point (pI)

72.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSF_DNA-bind PF00447 26 - 123 8.3e-27 HSF-type DNA-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 135
AclWI GGATC 1 cut(s) 18
AcsI RAATTY 1 cut(s) 308
AcuI CTGAAG 1 cut(s) 515
AfeI AGCGCT 1 cut(s) 385
AgsI TTSAA 4 cut(s) 232, 283, 313, 553
AhlI ACTAGT 1 cut(s) 422
AluBI AGCT 5 cut(s) 211, 252, 265, 529, 571
AluI AGCT 5 cut(s) 211, 252, 265, 529, 571
Alw21I GWGCWC 2 cut(s) 213, 422
Alw26I GTCTC 1 cut(s) 195
AlwI GGATC 1 cut(s) 18
Aor51HI AGCGCT 1 cut(s) 385
AoxI GGCC 1 cut(s) 433
ApeKI GCWGC 3 cut(s) 132, 458, 484
ApoI RAATTY 1 cut(s) 308
AspLEI GCGC 1 cut(s) 386
AsuHPI GGTGA 2 cut(s) 40, 285
AxyI CCTNAGG 1 cut(s) 509
BanII GRGCYC 1 cut(s) 213
BauI CACGAG 1 cut(s) 317
BbsI GAAGAC 1 cut(s) 126
Bbv12I GWGCWC 2 cut(s) 213, 422
BbvI GCAGC 3 cut(s) 144, 470, 471
BccI CCATC 3 cut(s) 122, 297, 612
BcoDI GTCTC 1 cut(s) 195
BcuI ACTAGT 1 cut(s) 422
BfaI CTAG 3 cut(s) 101, 423, 560
BfoI RGCGCY 1 cut(s) 387
BisI GCNGC 3 cut(s) 133, 459, 485
BlsI GCNGC 3 cut(s) 134, 460, 486
BmsI GCATC 3 cut(s) 326, 352, 525
BpiI GAAGAC 1 cut(s) 126
BpmI CTGGAG 2 cut(s) 232, 374
Bsa29I ATCGAT 1 cut(s) 613
BsaI GGTCTC 1 cut(s) 195
BsaJI CCNNGG 1 cut(s) 34
BsaWI WCCGGW 1 cut(s) 391
Bse118I RCCGGY 1 cut(s) 339
Bse1I ACTGG 2 cut(s) 357, 436
Bse21I CCTNAGG 1 cut(s) 509
BseCI ATCGAT 1 cut(s) 613
BseDI CCNNGG 1 cut(s) 34
BseGI GGATG 1 cut(s) 341
BseMII CTCAG 3 cut(s) 189, 219, 560
BseNI ACTGG 2 cut(s) 357, 436
BseRI GAGGAG 1 cut(s) 509
BseXI GCAGC 3 cut(s) 144, 470, 471
BshFI GGCC 1 cut(s) 435
BshVI ATCGAT 1 cut(s) 613
BsiHKAI GWGCWC 2 cut(s) 213, 422
BsiSI CCGG 2 cut(s) 340, 392
BsmAI GTCTC 1 cut(s) 195
BsnI GGCC 1 cut(s) 435
Bso31I GGTCTC 1 cut(s) 195
Bsp1286I GDGCHC 2 cut(s) 213, 422
Bsp143I GATC 3 cut(s) 10, 143, 357
Bsp19I CCATGG 1 cut(s) 34
BspACI CCGC 1 cut(s) 135
BspANI GGCC 1 cut(s) 435
BspCNI CTCAG 3 cut(s) 190, 218, 559
BspDI ATCGAT 1 cut(s) 613
BspPI GGATC 1 cut(s) 18
BspTNI GGTCTC 1 cut(s) 195
BsrFI RCCGGY 1 cut(s) 339
BsrI ACTGG 2 cut(s) 357, 436
BssAI RCCGGY 1 cut(s) 339
BssECI CCNNGG 1 cut(s) 34
BssMI GATC 3 cut(s) 10, 143, 357
BssSI CACGAG 1 cut(s) 317
BssT1I CCWWGG 1 cut(s) 34
Bst2BI CACGAG 1 cut(s) 317
Bst6I CTCTTC 2 cut(s) 108, 408
BstC8I GCNNGC 2 cut(s) 263, 341
BstDEI CTNAG 4 cut(s) 198, 205, 509, 546
BstDSI CCRYGG 1 cut(s) 34
BstF5I GGATG 1 cut(s) 341
BstH2I RGCGCY 1 cut(s) 387
BstHHI GCGC 1 cut(s) 386
BstKTI GATC 3 cut(s) 13, 146, 360
BstMAI GTCTC 1 cut(s) 195
BstMBI GATC 3 cut(s) 10, 143, 357
BstMWI GCNNNNNNNGC 4 cut(s) 217, 258, 381, 535
BstV1I GCAGC 3 cut(s) 144, 470, 471
BstV2I GAAGAC 1 cut(s) 126
BstXI CCANNNNNNTGG 1 cut(s) 303
Bsu15I ATCGAT 1 cut(s) 613
Bsu36I CCTNAGG 1 cut(s) 509
BsuRI GGCC 1 cut(s) 435
BsuTUI ATCGAT 1 cut(s) 613
BtgI CCRYGG 1 cut(s) 34
BtsCI GGATG 1 cut(s) 341
Cac8I GCNNGC 2 cut(s) 263, 341
CfoI GCGC 1 cut(s) 386
Cfr10I RCCGGY 1 cut(s) 339
ClaI ATCGAT 1 cut(s) 613
CviAII CATG 3 cut(s) 35, 156, 448
CviJI RGCY 8 cut(s) 66, 211, 252, 265, 407, 435, 529, 571
CviKI_1 RGCY 8 cut(s) 66, 211, 252, 265, 407, 435, 529, 571
DdeI CTNAG 4 cut(s) 198, 205, 509, 546
DpnI GATC 3 cut(s) 12, 145, 359
DpnII GATC 3 cut(s) 10, 143, 357
Eam1104I CTCTTC 2 cut(s) 108, 408
EarI CTCTTC 2 cut(s) 108, 408
Ecl136II GAGCTC 1 cut(s) 211
Eco130I CCWWGG 1 cut(s) 34
Eco24I GRGCYC 1 cut(s) 213
Eco31I GGTCTC 1 cut(s) 195
Eco47III AGCGCT 1 cut(s) 385
Eco53kI GAGCTC 1 cut(s) 211
Eco57I CTGAAG 1 cut(s) 515
Eco81I CCTNAGG 1 cut(s) 509
EcoICRI GAGCTC 1 cut(s) 211
EcoRI GAATTC 1 cut(s) 308
EcoT14I CCWWGG 1 cut(s) 34
EcoT38I GRGCYC 1 cut(s) 213
ErhI CCWWGG 1 cut(s) 34
FaeI CATG 3 cut(s) 38, 159, 451
FatI CATG 3 cut(s) 34, 155, 447
Fnu4HI GCNGC 3 cut(s) 133, 459, 485
FokI GGATG 1 cut(s) 348
FriOI GRGCYC 1 cut(s) 213
Fsp4HI GCNGC 3 cut(s) 133, 459, 485
FspBI CTAG 3 cut(s) 101, 423, 560
GlaI GCGC 1 cut(s) 385
GluI GCNGC 3 cut(s) 133, 459, 485
GsuI CTGGAG 2 cut(s) 232, 374
HaeII RGCGCY 1 cut(s) 387
HaeIII GGCC 1 cut(s) 435
HapII CCGG 2 cut(s) 340, 392
HhaI GCGC 1 cut(s) 386
Hin1II CATG 3 cut(s) 38, 159, 451
Hin6I GCGC 1 cut(s) 384
HinP1I GCGC 1 cut(s) 384
HinfI GANTC 2 cut(s) 279, 472
HpaII CCGG 2 cut(s) 340, 392
HphI GGTGA 2 cut(s) 40, 285
Hpy166II GTNNAC 1 cut(s) 31
Hpy188I TCNGA 6 cut(s) 25, 48, 208, 366, 412, 534
Hpy188III TCNNGA 2 cut(s) 283, 328
Hpy8I GTNNAC 1 cut(s) 31
HpyAV CCTTC 1 cut(s) 325
HpyCH4IV ACGT 1 cut(s) 89
HpyCH4V TGCA 3 cut(s) 458, 538, 598
HpyF10VI GCNNNNNNNGC 4 cut(s) 217, 258, 381, 535
HpyF3I CTNAG 4 cut(s) 198, 205, 509, 546
HpySE526I ACGT 1 cut(s) 89
Hsp92II CATG 3 cut(s) 38, 159, 451
HspAI GCGC 1 cut(s) 384
KroI GCCGGC 1 cut(s) 339
KroNI GCCGGC 1 cut(s) 341
Kzo9I GATC 3 cut(s) 10, 143, 357
LmnI GCTCC 1 cut(s) 526
LpnPI CCDG 9 cut(s) 81, 207, 262, 275, 338, 341, 353, 405, 417
Lsp1109I GCAGC 3 cut(s) 144, 470, 471
LweI GCATC 3 cut(s) 326, 352, 525
MaeI CTAG 3 cut(s) 101, 423, 560
MaeII ACGT 1 cut(s) 89
MalI GATC 3 cut(s) 12, 145, 359
MboI GATC 3 cut(s) 10, 143, 357
MboII GAAGA 7 cut(s) 119, 122, 125, 131, 137, 379, 425
MfeI CAATTG 1 cut(s) 147
MhlI GDGCHC 2 cut(s) 213, 422
MluCI AATT 2 cut(s) 147, 308
MmeI TCCRAC 1 cut(s) 555
MnlI CCTC 6 cut(s) 19, 48, 97, 109, 160, 487
MroNI GCCGGC 1 cut(s) 339
MseI TTAA 2 cut(s) 267, 438
MspA1I CMGCKG 1 cut(s) 135
MspI CCGG 2 cut(s) 340, 392
MunI CAATTG 1 cut(s) 147
MwoI GCNNNNNNNGC 4 cut(s) 217, 258, 381, 535
NaeI GCCGGC 1 cut(s) 341
NcoI CCATGG 1 cut(s) 34
NdeII GATC 3 cut(s) 10, 143, 357
NgoMIV GCCGGC 1 cut(s) 339
NlaIII CATG 3 cut(s) 38, 159, 451
PdiI GCCGGC 1 cut(s) 341
PfeI GAWTC 2 cut(s) 279, 472
PkrI GCNGC 3 cut(s) 134, 460, 486
Psp124BI GAGCTC 1 cut(s) 213
SacI GAGCTC 1 cut(s) 213
SaqAI TTAA 2 cut(s) 267, 438
SatI GCNGC 3 cut(s) 133, 459, 485
Sau3AI GATC 3 cut(s) 10, 143, 357
SduI GDGCHC 2 cut(s) 213, 422
SetI ASST 9 cut(s) 30, 92, 213, 254, 267, 275, 510, 531, 573
SfaNI GCATC 3 cut(s) 326, 352, 525
SpeI ACTAGT 1 cut(s) 422
Sse9I AATT 2 cut(s) 147, 308
SsiI CCGC 1 cut(s) 135
SspMI CTAG 3 cut(s) 101, 423, 560
SstI GAGCTC 1 cut(s) 213
StyI CCWWGG 1 cut(s) 34
TaiI ACGT 1 cut(s) 92
TaqI TCGA 4 cut(s) 53, 83, 442, 613
TasI AATT 2 cut(s) 147, 308
TfiI GAWTC 2 cut(s) 279, 472
Tru1I TTAA 2 cut(s) 267, 438
Tru9I TTAA 2 cut(s) 267, 438
TseI GCWGC 3 cut(s) 132, 458, 484
TspDTI ATGAA 1 cut(s) 464
XapI RAATTY 1 cut(s) 308
XspI CTAG 3 cut(s) 101, 423, 560
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.