pycom10g24840

Tetraspanin family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0014685
Physical Location & Seq
Forward (+)
26580027 .. 26582175
2149 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 768 bp
ATGCTTGCAAGTTTTCTTACTTTGAAGCGCTTTTATTTGTCGGAAAACATTTTCAGAAGCCGAGATTTGATTGGTGGGATTAATCTTGCTTTGTCCAAGTTGAAGAAGCTTTGGAAGTCACTAGCTACTACAATGGCGGCAACAGTTGCAAGAATCTGCCTGCAGTCAGTGCTGAAAGCAGTGAACTCACTTTTGGGAATGGTTGGAATTGCAATGATTTTGTATGGTCTTTGGATGGTTAGGGTTTGGCTGAGGGACTTTGACGGTTCACCCTTTGATCATCATAATGTTTTTCCTCCATGGTTTATATGCACTTCTCTTGGCGCTGGATTTACCTTATGCATAGTAACATGCATAGGTCTTGTTGCTGCAAAGTATGCAAATGGTTGTTGCCTCTCTTTTTACATGTTGATCATCTTTTTGGTGATTCTATTGGAGTTTGCAGTGGCTGCAGATATACTTTTAAATTCAGAATGGGAGAAGGATTTACCATATGACCCGACGGGAAAGTTCAATGATTTCAAAGATTTTCTCGAGTCAAACTTAGACATATTTCAATGGATAGGTTTGTTCATCATTTTAGCACAGGGGTTGTCAATCGTACTAGCCACAGCTCTAAGGTCTGCAGGGCCAAACCGAAGATCCAGCTACGACGGCGATGAAGAGTGTCCCCCGGAAAGACTCCCGCTCATAAACCCTAATGGTCACCGGCCGGAATACATTATCGCTGCCAAAAATGGTAATTGGAATGTAAGAATTCACTCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Tetraspannin

Protein Analysis

256

Amino Acids

28.57

Weight (kDa)

8.14

Isoelectric Point (pI)

50.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tetraspanin PF00335 57 - 155 7.1e-10 Tetraspanin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 688
AciI CCGC 2 cut(s) 137, 686
AclWI GGATC 1 cut(s) 636
AcoI YGGCCR 1 cut(s) 710
AcsI RAATTY 2 cut(s) 466, 756
AfaI GTAC 1 cut(s) 603
AfeI AGCGCT 1 cut(s) 29
AflIII ACRYGT 1 cut(s) 405
AgsI TTSAA 5 cut(s) 25, 103, 514, 523, 557
AjuI GAANNNNNNNTTGG 2 cut(s) 176, 208
AluBI AGCT 4 cut(s) 109, 125, 614, 648
AluI AGCT 4 cut(s) 109, 125, 614, 648
AlwI GGATC 1 cut(s) 636
AlwNI CAGNNNCTG 1 cut(s) 449
Ama87I CYCGRG 1 cut(s) 533
Aor51HI AGCGCT 1 cut(s) 29
AoxI GGCC 2 cut(s) 629, 710
ApeKI GCWGC 3 cut(s) 368, 449, 728
ApoI RAATTY 2 cut(s) 466, 756
AseI ATTAAT 1 cut(s) 81
AspLEI GCGC 2 cut(s) 30, 326
AspS9I GGNCC 1 cut(s) 629
AsuC2I CCSGG 1 cut(s) 674
AsuHPI GGTGA 3 cut(s) 261, 436, 698
AvaI CYCGRG 1 cut(s) 533
BbvCI CCTCAGC 1 cut(s) 251
BbvI GCAGC 3 cut(s) 355, 436, 715
BccI CCATC 1 cut(s) 229
BceAI ACGGC 1 cut(s) 670
BclI TGATCA 2 cut(s) 277, 411
BcnI CCSGG 1 cut(s) 674
BfaI CTAG 2 cut(s) 122, 605
BfmI CTRYAG 3 cut(s) 161, 450, 624
BfoI RGCGCY 2 cut(s) 31, 327
BisI GCNGC 4 cut(s) 138, 369, 450, 729
BlsI GCNGC 4 cut(s) 139, 370, 451, 730
Bme1390I CCNGG 1 cut(s) 674
BmeT110I CYCGRG 1 cut(s) 533
BmgT120I GGNCC 1 cut(s) 629
BmrFI CCNGG 1 cut(s) 674
Bpu10I CCTNAGC 1 cut(s) 251
BpuMI CCSGG 1 cut(s) 674
BsaJI CCNNGG 2 cut(s) 299, 672
Bse118I RCCGGY 1 cut(s) 708
Bse3DI GCAATG 1 cut(s) 219
BseDI CCNNGG 2 cut(s) 299, 672
BseGI GGATG 1 cut(s) 240
BseMI GCAATG 1 cut(s) 219
BseMII CTCAG 1 cut(s) 242
BseX3I CGGCCG 1 cut(s) 710
BseXI GCAGC 3 cut(s) 355, 436, 715
Bsh1285I CGRYCG 1 cut(s) 713
BshFI GGCC 2 cut(s) 631, 712
BsiEI CGRYCG 1 cut(s) 713
BsiHKCI CYCGRG 1 cut(s) 533
BsiSI CCGG 3 cut(s) 674, 709, 713
BslFI GGGAC 2 cut(s) 269, 654
BsmFI GGGAC 2 cut(s) 269, 654
BsnI GGCC 2 cut(s) 631, 712
BsoBI CYCGRG 1 cut(s) 533
Bsp143I GATC 3 cut(s) 277, 411, 641
Bsp19I CCATGG 1 cut(s) 299
BspACI CCGC 2 cut(s) 137, 686
BspANI GGCC 2 cut(s) 631, 712
BspCNI CTCAG 1 cut(s) 243
BspMAI CTGCAG 3 cut(s) 165, 454, 628
BspPI GGATC 1 cut(s) 636
BsrBI CCGCTC 1 cut(s) 688
BsrDI GCAATG 1 cut(s) 219
BsrFI RCCGGY 1 cut(s) 708
BssAI RCCGGY 1 cut(s) 708
BssECI CCNNGG 2 cut(s) 299, 672
BssMI GATC 3 cut(s) 277, 411, 641
BssT1I CCWWGG 1 cut(s) 299
Bst4CI ACNGT 2 cut(s) 145, 266
Bst6I CTCTTC 1 cut(s) 657
BstAPI GCANNNNNTGC 4 cut(s) 146, 169, 377, 449
BstC8I GCNNGC 2 cut(s) 6, 161
BstDEI CTNAG 3 cut(s) 251, 544, 617
BstDSI CCRYGG 1 cut(s) 299
BstEII GGTNACC 1 cut(s) 704
BstF5I GGATG 1 cut(s) 240
BstH2I RGCGCY 2 cut(s) 31, 327
BstHHI GCGC 2 cut(s) 30, 326
BstKTI GATC 3 cut(s) 280, 414, 644
BstMBI GATC 3 cut(s) 277, 411, 641
BstMCI CGRYCG 1 cut(s) 713
BstMWI GCNNNNNNNGC 5 cut(s) 146, 169, 377, 449, 654
BstNSI RCATGY 2 cut(s) 354, 409
BstPI GGTNACC 1 cut(s) 704
BstSCI CCNGG 1 cut(s) 672
BstSFI CTRYAG 3 cut(s) 161, 450, 624
BstV1I GCAGC 3 cut(s) 355, 436, 715
BstX2I RGATCY 1 cut(s) 641
BstYI RGATCY 1 cut(s) 641
BstZI CGGCCG 1 cut(s) 710
BsuRI GGCC 2 cut(s) 631, 712
BtgI CCRYGG 1 cut(s) 299
BtgZI GCGATG 1 cut(s) 672
BtsCI GGATG 1 cut(s) 240
BtsI GCAGTG 2 cut(s) 186, 450
BtsIMutI CAGTG 3 cut(s) 174, 186, 450
Cac8I GCNNGC 2 cut(s) 6, 161
CaiI CAGNNNCTG 1 cut(s) 449
CfoI GCGC 2 cut(s) 30, 326
Cfr10I RCCGGY 1 cut(s) 708
Cfr13I GGNCC 1 cut(s) 629
Csp6I GTAC 1 cut(s) 602
CviAII CATG 3 cut(s) 300, 351, 406
CviQI GTAC 1 cut(s) 602
DdeI CTNAG 3 cut(s) 251, 544, 617
DpnI GATC 3 cut(s) 279, 413, 643
DpnII GATC 3 cut(s) 277, 411, 641
DraI TTTAAA 1 cut(s) 465
EaeI YGGCCR 1 cut(s) 710
EagI CGGCCG 1 cut(s) 710
Eam1104I CTCTTC 1 cut(s) 657
EarI CTCTTC 1 cut(s) 657
EclXI CGGCCG 1 cut(s) 710
Eco130I CCWWGG 1 cut(s) 299
Eco47III AGCGCT 1 cut(s) 29
Eco52I CGGCCG 1 cut(s) 710
Eco88I CYCGRG 1 cut(s) 533
Eco91I GGTNACC 1 cut(s) 704
EcoO65I GGTNACC 1 cut(s) 704
EcoRI GAATTC 1 cut(s) 756
EcoT14I CCWWGG 1 cut(s) 299
EcoT22I ATGCAT 2 cut(s) 344, 356
ErhI CCWWGG 1 cut(s) 299
FaeI CATG 3 cut(s) 303, 354, 409
FaqI GGGAC 2 cut(s) 269, 654
FatI CATG 3 cut(s) 299, 350, 405
FauI CCCGC 1 cut(s) 693
FauNDI CATATG 1 cut(s) 493
FbaI TGATCA 2 cut(s) 277, 411
Fnu4HI GCNGC 4 cut(s) 138, 369, 450, 729
FokI GGATG 1 cut(s) 247
Fsp4HI GCNGC 4 cut(s) 138, 369, 450, 729
FspBI CTAG 2 cut(s) 122, 605
GlaI GCGC 2 cut(s) 29, 325
GluI GCNGC 4 cut(s) 138, 369, 450, 729
HaeII RGCGCY 2 cut(s) 31, 327
HaeIII GGCC 2 cut(s) 631, 712
HapII CCGG 3 cut(s) 674, 709, 713
HhaI GCGC 2 cut(s) 30, 326
Hin1II CATG 3 cut(s) 303, 354, 409
Hin6I GCGC 2 cut(s) 28, 324
HinP1I GCGC 2 cut(s) 28, 324
HindIII AAGCTT 1 cut(s) 107
HinfI GANTC 4 cut(s) 153, 427, 536, 681
HpaII CCGG 3 cut(s) 674, 709, 713
HphI GGTGA 3 cut(s) 261, 436, 698
Hpy166II GTNNAC 2 cut(s) 184, 269
Hpy188I TCNGA 3 cut(s) 43, 56, 472
Hpy188III TCNNGA 1 cut(s) 533
Hpy8I GTNNAC 2 cut(s) 184, 269
Hpy99I CGWCG 2 cut(s) 505, 656
HpyAV CCTTC 1 cut(s) 475
HpyCH4III ACNGT 2 cut(s) 145, 266
HpyF10VI GCNNNNNNNGC 5 cut(s) 146, 169, 377, 449, 654
HpyF3I CTNAG 3 cut(s) 251, 544, 617
Hsp92II CATG 3 cut(s) 303, 354, 409
HspAI GCGC 2 cut(s) 28, 324
Ksp22I TGATCA 2 cut(s) 277, 411
Kzo9I GATC 3 cut(s) 277, 411, 641
LpnPI CCDG 8 cut(s) 173, 312, 572, 612, 658, 687, 722, 726
Lsp1109I GCAGC 3 cut(s) 355, 436, 715
MaeI CTAG 2 cut(s) 122, 605
MaeIII GTNAC 3 cut(s) 117, 346, 704
MalI GATC 3 cut(s) 279, 413, 643
MbiI CCGCTC 1 cut(s) 688
MboI GATC 3 cut(s) 277, 411, 641
MboII GAAGA 3 cut(s) 115, 651, 674
MflI RGATCY 1 cut(s) 641
MluCI AATT 4 cut(s) 207, 466, 742, 756
MlyI GAGTC 2 cut(s) 545, 675
MmeI TCCRAC 2 cut(s) 21, 184
MnlI CCTC 3 cut(s) 246, 306, 404
Mph1103I ATGCAT 2 cut(s) 344, 356
MseI TTAA 2 cut(s) 81, 464
MslI CAYNNNNRTG 1 cut(s) 285
MspI CCGG 3 cut(s) 674, 709, 713
MspR9I CCNGG 1 cut(s) 674
MwoI GCNNNNNNNGC 5 cut(s) 146, 169, 377, 449, 654
NciI CCSGG 1 cut(s) 674
NcoI CCATGG 1 cut(s) 299
NdeI CATATG 1 cut(s) 493
NdeII GATC 3 cut(s) 277, 411, 641
NlaIII CATG 3 cut(s) 303, 354, 409
NmeAIII GCCGAG 1 cut(s) 86
NmuCI GTSAC 2 cut(s) 117, 704
NsiI ATGCAT 2 cut(s) 344, 356
NspI RCATGY 2 cut(s) 354, 409
PaeR7I CTCGAG 1 cut(s) 533
PciI ACATGT 1 cut(s) 405
PfeI GAWTC 2 cut(s) 153, 427
PkrI GCNGC 4 cut(s) 139, 370, 451, 730
PleI GAGTC 2 cut(s) 544, 675
PpsI GAGTC 2 cut(s) 544, 675
PscI ACATGT 1 cut(s) 405
PshBI ATTAAT 1 cut(s) 81
PspEI GGTNACC 1 cut(s) 704
PspPI GGNCC 1 cut(s) 629
PstI CTGCAG 3 cut(s) 165, 454, 628
PstNI CAGNNNCTG 1 cut(s) 449
PsuI RGATCY 1 cut(s) 641
RsaI GTAC 1 cut(s) 603
RsaNI GTAC 1 cut(s) 602
RseI CAYNNNNRTG 1 cut(s) 285
SaqAI TTAA 2 cut(s) 81, 464
SatI GCNGC 4 cut(s) 138, 369, 450, 729
Sau3AI GATC 3 cut(s) 277, 411, 641
Sau96I GGNCC 1 cut(s) 629
SchI GAGTC 2 cut(s) 545, 675
ScrFI CCNGG 1 cut(s) 674
SetI ASST 8 cut(s) 111, 127, 338, 361, 568, 616, 623, 650
SfcI CTRYAG 3 cut(s) 161, 450, 624
Sfr274I CTCGAG 1 cut(s) 533
SlaI CTCGAG 1 cut(s) 533
SmiMI CAYNNNNRTG 1 cut(s) 285
SmlI CTYRAG 1 cut(s) 533
SmoI CTYRAG 1 cut(s) 533
Sse9I AATT 4 cut(s) 207, 466, 742, 756
SsiI CCGC 2 cut(s) 137, 686
SspMI CTAG 2 cut(s) 122, 605
StyD4I CCNGG 1 cut(s) 672
StyI CCWWGG 1 cut(s) 299
TaaI ACNGT 2 cut(s) 145, 266
TaqI TCGA 1 cut(s) 534
TasI AATT 4 cut(s) 207, 466, 742, 756
TauI GCSGC 1 cut(s) 140
TfiI GAWTC 2 cut(s) 153, 427
Tru1I TTAA 2 cut(s) 81, 464
Tru9I TTAA 2 cut(s) 81, 464
TscAI CASTG 3 cut(s) 174, 186, 450
TseFI GTSAC 2 cut(s) 117, 704
TseI GCWGC 3 cut(s) 368, 449, 728
Tsp45I GTSAC 2 cut(s) 117, 704
TspDTI ATGAA 2 cut(s) 562, 675
TspRI CASTG 3 cut(s) 174, 186, 450
VspI ATTAAT 1 cut(s) 81
XapI RAATTY 2 cut(s) 466, 756
XceI RCATGY 2 cut(s) 354, 409
XhoI CTCGAG 1 cut(s) 533
XspI CTAG 2 cut(s) 122, 605
Zsp2I ATGCAT 2 cut(s) 344, 356
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.